CN114394678B - Sewage treatment material, preparation method and application - Google Patents

Sewage treatment material, preparation method and application Download PDF

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CN114394678B
CN114394678B CN202210054316.3A CN202210054316A CN114394678B CN 114394678 B CN114394678 B CN 114394678B CN 202210054316 A CN202210054316 A CN 202210054316A CN 114394678 B CN114394678 B CN 114394678B
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spinning
strain
sewage treatment
liquid
treatment material
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CN114394678A (en
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常兴华
张斌
金胜明
白泽惠
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Central South University
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Central South University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/20—Treatment of water, waste water, or sewage by degassing, i.e. liberation of dissolved gases
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C02F3/2806—Anaerobic processes using solid supports for microorganisms
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
    • D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/163—Nitrates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Textile Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biological Treatment Of Waste Water (AREA)
  • Water Treatment By Electricity Or Magnetism (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a sewage treatment material, a preparation method and application. The preparation method of the sewage treatment material comprises the following steps: uniformly mixing the spinning solution, a carbon source substance and strains of functional bacteria, wherein the functional bacteria are denitrifying bacteria; the carbon source substance is a substance which can activate and provide energy for the activity of the strain; and separating the liquid in the uniformly mixed liquid, and performing electrostatic spinning on the liquid to obtain the sewage treatment material. The strain of the functional bacteria is immobilized in the spinning nano-fiber formed by the spinning solution, the combination degree of the functional bacteria formed by the strain germination and the spinning nano-fiber is high, secondary pollution caused by leakage is not easy to occur, and therefore, the post-treatment is simple. Meanwhile, the strains are uniformly dispersed, the active area of the functional bacteria is large, the functional bacteria can be in contact with nitrate in sewage sufficiently, the sewage treatment effect is good, the activity of the strains can be ensured, and the experimental period is shortened.

Description

Sewage treatment material, preparation method and application
Technical Field
The invention relates to the field of sewage treatment, in particular to a sewage treatment material, a preparation method and application.
Background
In recent years, with the acceleration of urbanization and industrialization processes in China, water pollution is increasingly serious, and the quality of water resources is seriously reduced. Due to the increase of sewage discharge amount, the sewage cannot be completely and properly treated before being discharged, and the shortage of water resources is further increased. The types of pollutants in the water body are numerous and complicated, and comprise solid pollutants, organic pollutants, toxic pollutants, inorganic pollutants and the like. A very common contaminant is nitrate. Nitrate is a great hazard to both nature and human health.
The chemical precipitation method, the electrolysis method, the physical adsorption method and the like which are widely applied at present are difficult to deal with the nitrate impact problem from domestic sewage or industrial wastewater in the sewage treatment industry due to the problems of high cost, narrow treatment range and the like. The traditional biological removal method is the most widely used means at present, and the technology is mature. However, the required conditions are relatively severe, and the post-treatment process is relatively troublesome.
Disclosure of Invention
Therefore, it is necessary to provide a sewage treatment material, a preparation method and an application aiming at the problems of relatively harsh reaction conditions and troublesome post-treatment process of the traditional biological removal method.
In a first aspect of the present application, a method for preparing a sewage treatment material is provided, comprising the steps of:
uniformly mixing the spinning solution, a carbon source substance and strains of functional bacteria, wherein the functional bacteria are denitrifying bacteria; the carbon source substance is a substance which can be activated and can provide energy for the strain activity;
and separating the liquid in the uniformly mixed liquid, and performing electrostatic spinning on the liquid to obtain the sewage treatment material.
Optionally, the mass ratio of the carbon source substance to the strain is (2-6): 5, the carbon source substance is one or more of glucose, sucrose, sodium acetate, methanol and ethanol.
Optionally, the spinning solution comprises a spinning solute and an organic solvent, the mass percentage concentration of the spinning solution is 10-15%, and the mass ratio of the spinning solute to the strain is (2-3): 1.
optionally, the spinning solute is polyacrylonitrile or polyethylene, and the organic solvent is N, N-dimethylformamide.
Optionally, the step of uniformly mixing the spinning solution, the carbon source substance and the strains of the functional bacteria comprises:
and uniformly stirring the spinning solution and the carbon source substance, adding the strain, and stirring for 2-3 hours.
Optionally, the step of separating the liquid in the uniformly mixed solution includes:
and standing the uniformly mixed solution for 20-40 minutes to separate the liquid from the lower-layer substances.
Optionally, the step of performing electrostatic spinning on the liquid to obtain the sewage treatment material includes:
adding the separated liquid in the mixed liquid into the injector, and discharging air bubbles;
coating a collecting film on a collecting cylinder;
assembling a conduit at a liquid outlet of a syringe, wherein one end of the conduit far away from the syringe is provided with a needle which is aligned with the collecting membrane;
and applying high-voltage direct current between the needle head and the collecting cylinder to propel the injector, so that the spinning nano fibers are formed on the collecting membrane.
In a second aspect of the application, a sewage treatment material prepared by the preparation method is provided.
In a third aspect of the present application, there is provided a use of the above sewage treatment material in sewage treatment, comprising the following steps:
and putting the sewage treatment material into sewage, wherein the sewage is sewage containing nitrate.
Optionally, the method further comprises deoxidizing the sewage.
The preparation method of the sewage treatment material takes the spinning solution, the carbon source substance and the strains of the functional bacteria as raw materials to prepare the sewage treatment material. The strain of the functional bacteria is immobilized in the spinning nano-fiber formed by the spinning solution, the combination degree of the functional bacteria formed by the strain germination and the spinning nano-fiber is high, secondary pollution caused by leakage is not easy to occur, and therefore, the post-treatment is simple. Meanwhile, the strain is uniformly dispersed, the active area of the functional strain is large, the functional strain can be in contact with nitrate in sewage sufficiently, the sewage treatment effect is good, the activity of the strain can be ensured, and the experimental period is shortened.
Drawings
FIG. 1a is a graph showing the time dependence of the nitrate concentration in example 1; FIG. 1b is a graph of bacterial density as a function of time for example 1; FIG. 1c is a plot of nitrate concentration as a function of bacteria density for example 1;
FIG. 2a is a graph showing the time dependence of the nitrate concentration in example 2; FIG. 2b is a graph of bacterial density as a function of time for example 2; FIG. 2c is a plot of nitrate concentration as a function of bacteria density for example 2;
FIG. 3a is a graph showing the time dependence of nitrate concentration in example 3; FIG. 3b is a graph of bacterial density as a function of time for example 3; FIG. 3c is a plot of nitrate concentration as a function of bacteria density for example 3;
FIG. 4a is a plot of nitrate concentration versus time for example 4; FIG. 4b is a graph of bacterial density as a function of time for example 4; FIG. 4c is a plot of nitrate concentration as a function of bacteria density for example 4;
FIG. 5a is a graph showing the time-dependent change in nitrate concentration of example 5; FIG. 5b is a graph of bacterial density as a function of time for example 5; FIG. 5c is a plot of nitrate concentration as a function of bacteria density for example 5;
FIG. 6 is a standard curve of the nitrate-absorbance curve of the present application.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.
In a first aspect of the present application, a method for preparing a sewage treatment material is provided, which comprises the following steps:
uniformly mixing the spinning solution, a carbon source substance and strains of functional bacteria, wherein the functional bacteria are denitrifying bacteria; the carbon source substance is a substance which can activate and provide energy for the activity of the strain;
and separating the liquid in the uniformly mixed liquid, and performing electrostatic spinning on the liquid to obtain the sewage treatment material.
In the above case, the spinning solution may be prepared first. The spinning solution is formed by mixing an organic polymer and an organic solvent, so that the spinning nanofiber is directly formed in the electrostatic spinning step. The spinning solution can also be formed by mixing a monomer, an initiator and an organic solvent, in the electrostatic spinning step, the monomer is firstly subjected to polymerization reaction to generate a polymer, and the polymer forms the spinning nanofiber.
The spinning solution, the carbon source substance and the strains of the functional bacteria are uniformly mixed to obtain a mixed solution, for example, the carbon source substance and the strains of the functional bacteria are simultaneously added into the spinning solution and uniformly mixed by stirring, or one of the carbon source substance and the strains of the functional bacteria is added into the spinning solution and uniformly mixed, and then the other substance is added. The mixed liquid contains strains of denitrifying bacteria. Strains of denitrifying bacteria are commercially available. The strain of denitrifying bacteria can be denitrifying bacteria produced by Henan Yuebao Biotechnology Limited. The carbon source substance can activate the strain and provide energy required by the life activity of the strain.
The mixed liquid can be allowed to stand or be centrifuged to separate the liquid and other substances. And (3) carrying out electrostatic spinning on the liquid component to obtain the sewage treatment material. The electrostatic spinning is a special form that high molecular fluid is subjected to electrostatic atomization in a strong electric field, and substances obtained by atomization and separation are polymer micro jet flows which can run for a long distance and are finally solidified into fibers. The electrostatic spinning can produce polymer fiber with nanometer diameter, so the polymer fiber has the advantages of large surface area and high void ratio.
The liquid components comprise spinning solution, carbon source substances and strains of functional bacteria. In this manner, the solute polymer in the spinning solution forms spun nanofibers. And carbon source substances and the functional bacteria are attached to the surface of the skeleton structure and/or embedded in the electrostatic spinning fiber. The spinning nano-fiber is used as a carrier or a medium, and the strains of the functional bacteria are immobilized in the spinning nano-fiber. When the sewage treatment material is integrally placed in sewage, the strains germinate and proliferate from the spinning nanofibers under the action of the carbon source substances. The strain and the electrostatic spinning fiber are tightly combined, so that the strain is not easy to fall off and leak to cause secondary pollution, and the post-treatment is simple. And the strain is uniformly dispersed, and the functional bacteria are uniformly dispersed in the electrostatic spinning fiber, so that the active area is large, the functional bacteria can be fully contacted with nitrate in sewage to perform denitration, the sewage treatment effect is good, the activity of the strain can be ensured, and the experimental period is shortened. The preparation method has the characteristics of convenient operation, low cost, controllable process, recyclable materials and the like.
The preparation method of the sewage treatment material takes the spinning solution, the carbon source substance and the strains of the functional bacteria as raw materials to prepare the sewage treatment material. The strain of the functional bacteria is immobilized in the spinning nano-fiber formed by the spinning solution, the combination degree of the functional bacteria formed by the strain germination and the spinning nano-fiber is high, secondary pollution caused by leakage is not easy to occur, and therefore, the post-treatment is simple. Meanwhile, the strains are uniformly dispersed, the active area of the functional bacteria is large, the functional bacteria can be in contact with nitrate in sewage sufficiently, the sewage treatment effect is good, the activity of the strains can be ensured, and the experimental period is shortened.
Optionally, the mass ratio of the carbon source substance to the strain is (2-6): 5, the carbon source substance is one or more of glucose, sucrose, sodium acetate, methanol and ethanol. Under the condition of the mass ratio, the carbon source substance can provide enough energy for the life activities of the strains, the multiplication speed of the strains is high, the activity is high, meanwhile, the waste of the carbon source substance can be avoided, and the cost is saved.
Optionally, the spinning solution comprises a spinning solute and an organic solvent, the mass percentage concentration of the spinning solution is 10-15%, and the mass ratio of the spinning solute to the strain is (2-3): 1.
under the mass percentage concentration of the spinning solution, the electrostatic spinning effect is better. The solute and the strain can be well dispersed in the solvent under the mass ratio, and the solvent waste can not be caused.
Optionally, the spinning solute is polyacrylonitrile or polyethylene, and the organic solvent is N, N-dimethylformamide.
The spinning solute has high yield of the prepared spinning nano-fiber and relatively few structural defects under the corresponding organic solvent. And polymer molecular chains are relatively stretched and are easy to form a spiral shape, so that the sewage treatment material has a better shape and is convenient to use. The spinning solute can also be a mixture of polyacrylonitrile or polyethylene, such as polyacrylonitrile: polyethylene =7:3 (mass ratio).
Optionally, the step of uniformly mixing the spinning solution, the carbon source substance and the strains of the functional bacteria comprises:
and after uniformly stirring the spinning solution and the carbon source substance, adding the strain and stirring for 2-3 hours.
The spinning solution and the carbon source substance are stirred uniformly, and then the strain is added, so that the strain can be better dispersed in the solution.
Optionally, the step of separating the liquid in the uniformly mixed liquid includes:
and standing the uniformly mixed solution for 20-40 minutes to separate the liquid from the lower-layer substances.
Because the nutrient is added in the production process of the strain, the nutrient has larger size and is easy to block during spinning, the nutrient is removed in a standing mode to ensure the smoothness and reliability of the electrostatic spinning process.
Optionally, the step of performing electrostatic spinning on the liquid to obtain the sewage treatment material includes:
the separated liquid in the mixed liquid is added into the syringe, and air bubbles are discharged.
The collecting film is coated on the collecting cylinder.
A conduit is arranged at the liquid outlet of the syringe, and the end of the conduit far away from the syringe is provided with a needle which is aligned with the collecting membrane.
And applying high-voltage direct current between the needle head and the collecting barrel to propel the injector, and forming spinning nano fibers on the collecting membrane.
The electrostatic spinning can be completed in an electrostatic spinning machine. The electrostatic spinning machine comprises an injector, a guide pipe, a needle head, a propulsion pump, a high-voltage direct-current power supply and a collecting barrel. For example, a completely dry syringe may be used to aspirate the supernatant separated liquid from the mixture and empty the syringe of air bubbles. The liquid outlet of the injector is connected with the needle head through a conduit. The syringe was placed in the propel pump with the needle aimed at the collection membrane covered by the collection canister. The collecting film is a thin paper, such as A4 paper. And connecting a high-voltage direct-current power supply, applying high voltage between the needle head and the collecting barrel, and pushing the injector by the propelling pump to enable the spinning solution sprayed by the needle head to form spinning nanofibers. The collecting drum rotates to collect the spinning nano-fibers of the collecting film, and when the spinning nano-fibers are more, the spinning nano-fibers are interwoven with one another to form a spinning nano-fiber film.
In a second aspect of the application, a sewage treatment material prepared by the preparation method is provided. The sewage treatment material has higher strain activity and strong nitrate treatment capacity in sewage, and the functional bacteria formed by strain germination and the spinning nano-fibers have high combination degree and are not easy to leak to cause secondary pollution, so the sewage treatment material is convenient to use and simple in post-treatment. Compared with the traditional biological denitrification technology, the material has the advantages of short treatment period, wide application range, strong adaptability, low cost, material recovery and the like.
In a third aspect of the present application, there is provided a use of the above-mentioned sewage treatment material in sewage treatment, comprising the following steps:
and putting the sewage treatment material into sewage, wherein the sewage is sewage containing nitrate.
The application of the sewage treatment material in sewage treatment is convenient to use and simple in post-treatment.
Optionally, the method further comprises removing oxygen from the sewage.
The technical solution of the present application is described below with reference to specific embodiments.
The nitrate standard curve is plotted as follows (see standard HJ-T346-2007 uv-vis spectrophotometry):
1. 0.50, 1.00, 2.00, 3.00, 4.00mL of nitrate nitrogen standard stock solution (0.100 mg of nitrate nitrogen per mL of standard stock solution) was added to each of 5 200mL volumetric flasks and diluted to the scale with fresh deionized water to concentrations of 0.25, 0.50, 1.00, 1.50, 2.00ppm of nitrate nitrogen.
2. The absorbance of the sample solution was measured at a wavelength of 210nm using a 20mm cuvette of optical path, with water as a reference.
3. From the several sets of data obtained, fitting was performed to plot a nitrate-absorbance curve, see fig. 6.
Example 1
Weighing 1.3g of PAN, dissolving in 10ml of DMF, stirring until the PAN is colorless and transparent (the mass fraction of the PAN is 12%), adding a certain amount of glucose, fully stirring, and adding 0.6g of strain, wherein the strain is denitrifying strain, and the mass of the glucose is as follows: mass =2 for the strain. Stirring for 2-3 hours. Standing for 30min, and then taking the upper layer liquid for electrostatic spinning to obtain the sewage treatment material.
The electrostatic spinning process comprises the following steps: 10ml of the supernatant liquid was aspirated with a 10ml completely dry syringe, air bubbled, and charged to an electrospinning machine. Adjusting the distance between the needle head and the collecting plate to be 15cm, assembling a guide pipe, using a 19-gauge needle head, coating the collecting cylinder with A4 paper, connecting a high-voltage direct-current power supply, and adding 17kV high voltage between the needle head and the collecting cylinder. Setting parameters: a 10ml injector, the advancing speed is 0.03ml/min, and the spinning time is 5h.
Placing the obtained sewage treatment material in 500mLNaNO 3 Solution (c (NO) 3 - ) =450ppm, and Ar was introduced in advance for 30min to remove oxygen). Standing for 18h, sampling every 2 hr, diluting, measuring absorbance by ultraviolet-visible spectrophotometry, and calculating to obtain NO in the sample 3 - The contents are shown in FIG. 1 (a) and Table 1. FIG. 1 (a) shows NO in the sample over a period of 0 to 18h 3 - NO content reduction is not obvious, NO 3 - The content change speed is relatively slow. NO in the sample within the time period of 18-20 h 3 - The content is obviously reduced, NO 3 - The content changes rapidly. NO in the sample within the time period of 20-24 h 3 - The content of NO tends to decrease, but the decrease tends to be gentle 3 - The content decrease rate gradually decreases. NO in the sample in the time period of 24-26 h 3 - In a downward trend of content, NO 3 - The content reduction speed is accelerated. Meanwhile, the number of denitrifying bacteria in a water sample was measured by a blood cell plate counting method, as shown in FIG. 1 (b), and a graph of the change in nitrate concentration with the density of bacteria, as shown in FIG. 1 (c), was plotted.
Example 2
Weighing 1.3g of PAN, dissolving in 10ml of DMF, stirring until the PAN is colorless and transparent (the mass fraction of the PAN is 12%), adding a certain amount of sucrose, fully stirring, and adding 0.6g of strain, wherein the strain is a denitrifying strain, and the mass of the sucrose is as follows: mass =3 of the strain. Stirring for 2-3 hours. And (3) standing for 30min, and then taking the upper layer liquid for electrostatic spinning, wherein the electrostatic spinning process is the same as that of the example 1, so as to obtain the sewage treatment material.
Placing the obtained sewage treatment material in 500mLNaNO 3 Solution (c (NO) 3 - ) =450ppm, and Ar was introduced in advance for 30min to remove oxygen). Standing for 18h, sampling every 2 hr, diluting, measuring absorbance by ultraviolet-visible spectrophotometry, and calculating to obtain NO in the sample 3 - The contents are shown in FIG. 2 (a) and Table 1. FIG. 2 (a) shows NO in the sample over a period of 0 to 18h 3 - NO significant reduction in content, NO 3 - The content change speed is slow. NO in the sample within the time period of 18-20 h 3 - The content is obviously reduced, NO 3 - The content changes rapidly. NO in the sample within the time period of 20-24 h 3 - The content of NO tends to decrease, but the decrease tends to be gentle 3 - The content decrease rate gradually decreases. NO in the sample in the time period of 24-26 h 3 - In a downward trend of content, NO 3 - The content reduction speed is accelerated. Meanwhile, the number of denitrifying bacteria in a water sample was measured by a blood cell plate counting method, as shown in FIG. 2 (b), and a graph of the change in nitrate concentration with the density of bacteria, as shown in FIG. 2 (c), was plotted.
Example 3
Weighing 1.3g of PAN, dissolving the PAN in 10ml of MF, stirring the PAN until the PAN is colorless and transparent (the mass fraction of the PAN is 12%), adding a certain amount of sodium acetate, fully stirring the mixture, and adding 0.6g of strain, wherein the strain is denitrifying strain, and the mass of the sodium acetate is as follows: mass = 4. Stirring for 2-3 hours. Standing for 30min, and then taking the upper layer liquid for electrostatic spinning, wherein the electrostatic spinning process is the same as that of example 1, so as to obtain the sewage treatment material.
Subjecting the obtained product toThe sewage treatment material is placed in 500mLNaNO 3 Solution (c (NO) 3 - ) =450ppm, remove oxygen by passing Ar for 30min in advance). Standing for 18h, sampling every 2 h, diluting, measuring absorbance by ultraviolet-visible spectrophotometry, and calculating to obtain NO from standard curve 3 - The contents are shown in FIG. 3 (a) and Table 1. FIG. 3 (a) shows NO in the sample over a period of 0 to 18h 3 - NO significant reduction in content, NO 3 - The content change speed is slow. NO in the sample within the time period of 18-20 h 3 - The content is obviously reduced, NO 3 - The content changes rapidly. NO in the sample within the time period of 20-24 h 3 - The content of NO tends to decrease, but the decrease tends to be gentle 3 - The content decrease rate gradually decreases. NO in the sample within the time period of 24-26 h 3 - The content of NO is in a descending trend 3 - The content reduction speed is accelerated. Meanwhile, the number of denitrifying bacteria in a water sample was measured by a blood cell plate counting method, as shown in FIG. 3 (b), and a graph of the change in nitrate concentration with the density of bacteria, as shown in FIG. 3 (c), was plotted.
Example 4
Weighing 1.3g of PAN, dissolving in 10ml of DMF, stirring until the PAN is colorless and transparent (the mass fraction of the PAN is 12%), adding a certain amount of methanol, fully stirring, and adding 0.6g of strain, wherein the strain is denitrifying strain, and the mass of the methanol is as follows: mass =5 of the strain, and stirring for 2-3 hours. Standing for 30min, and then taking the upper layer liquid for electrostatic spinning, wherein the electrostatic spinning process is the same as that of example 1, so as to obtain the sewage treatment material.
Placing the obtained sewage treatment material in 500mLNaNO 3 Solution (c (NO) 3 - ) =450ppm, remove oxygen by passing Ar for 30min in advance). Standing for 18h, sampling every 2 hr, diluting, measuring absorbance by ultraviolet-visible spectrophotometry, and calculating to obtain NO in the sample 3 - The contents are shown in FIG. 4 (a) and Table 1. FIG. 4 (a) shows NO in the sample over a period of 0 to 18h 3 - NO significant reduction in content, NO 3 - The content change speed is slow. NO in the sample within the time period of 18-20 h 3 - The content is obviously reduced, NO 3 - The content changes rapidly. NO in the sample within the time period of 20-24 h 3 - The content of NO tends to decrease, but the decrease tends to be gentle 3 - The content decrease rate gradually decreases. NO in the sample in the time period of 24-26 h 3 - The content of NO is in a descending trend 3 - The content reduction speed is accelerated. Meanwhile, the number of denitrifying bacteria in a water sample was measured by a blood cell plate counting method, as shown in FIG. 4 (b), and a graph of the change in nitrate concentration with the density of bacteria, as shown in FIG. 4 (c), was plotted.
Example 5
Weighing 1.3g of PAN, dissolving in 10ml of DMF, stirring until the PAN is colorless and transparent (the mass fraction of the PAN is 12%), adding a certain amount of ethanol, fully stirring, and adding 0.6g of strain, wherein the strain is a denitrifying strain, and the mass of the ethanol is as follows: mass =6 of the strain. Stirring for 2-3 hours. Standing for 30min, and then taking the upper layer liquid for electrostatic spinning, wherein the electrostatic spinning process is the same as that of example 1, so as to obtain the sewage treatment material.
Placing the obtained sewage treatment material in 500mLNaNO 3 Solution (c (NO) 3 - ) =450ppm, and Ar was introduced in advance for 30min to remove oxygen). Standing for 18h, sampling every 2 hr, diluting, measuring absorbance by ultraviolet-visible spectrophotometry, and calculating to obtain NO in the sample 3 - The contents are shown in FIG. 5 (a) and Table 1. FIG. 4 (a) shows NO in the sample over a period of 0 to 18h 3 - NO significant reduction in content, NO 3 - The content change speed is slow. NO in the sample within the time period of 18-20 h 3 - The content is obviously reduced, NO 3 - The content changes rapidly. NO in the sample within the time period of 20-24 h 3 - The content of NO tends to decrease, but the decrease tends to be gentle 3 - The content decrease rate gradually decreases. NO in the sample in the time period of 24-26 h 3 - The content of NO is in a descending trend 3 - The content reduction speed is accelerated. Meanwhile, the number of denitrifying bacteria in a water sample was measured by a blood cell plate counting method, as shown in FIG. 5 (b), and a graph of the change in nitrate concentration with the density of bacteria, as shown in FIG. 5 (c), was plotted.
TABLE 1 tables of nitrate concentration for examples 1 to 5
Figure BDA0003475566490000101
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (9)

1. The preparation method of the sewage treatment material is characterized by comprising the following steps:
uniformly mixing the spinning solution, a carbon source substance and strains of functional bacteria, wherein the functional bacteria are denitrifying bacteria; the carbon source substance is a substance which activates and provides energy for the activity of the strain;
separating liquid in the uniformly mixed liquid, and performing electrostatic spinning on the liquid to obtain the sewage treatment material;
the carbon source substance is one or more of glucose, sucrose, sodium acetate, methanol and ethanol, and the spinning solution comprises a spinning solute and an organic solvent; the spinning solute is polyacrylonitrile or polyethylene, and the organic solvent is N, N-dimethylformamide; and the solute polymer in the spinning solution forms spinning nano-fibers, and the carbon source substance and the functional bacteria are attached to the surface of the skeleton structure and/or embedded in the electrostatic spinning fibers.
2. The method according to claim 1, wherein the mass ratio of the carbon source substance to the strain is (2 to 6): 5.
3. the preparation method according to claim 1, wherein the mass percentage concentration of the spinning solution is 10 to 15%, and the mass ratio of the spinning solute to the strain is (2 to 3): 1.
4. the method according to claim 1, wherein the step of uniformly mixing the spinning solution, the carbon source material and the strain of the functional bacteria comprises:
and after uniformly stirring the spinning solution and the carbon source substance, adding the strain and stirring for 2-3 hours.
5. The method according to claim 1, wherein the step of separating the liquid in the uniformly mixed liquid comprises:
and standing the uniformly mixed solution for 20-40 minutes to separate the liquid from the lower-layer substances.
6. The method according to claims 1 to 5, wherein the step of electrospinning the liquid to obtain the sewage treatment material comprises:
adding the separated liquid in the mixed liquid into the injector, and discharging air bubbles;
coating a collecting film on a collecting cylinder;
assembling a conduit at a liquid outlet of a syringe, wherein one end of the conduit far away from the syringe is provided with a needle which is aligned with the collecting membrane;
and applying high-voltage direct current between the needle head and the collecting barrel to propel the injector, and forming spinning nano fibers on the collecting membrane.
7. A sewage treatment material produced by the production method according to any one of claims 1 to 6.
8. Use of the wastewater treatment material according to claim 7 in wastewater treatment, comprising the steps of:
and putting the sewage treatment material into sewage, wherein the sewage is sewage containing nitrate.
9. The use of claim 8, further comprising deoxygenating the wastewater.
CN202210054316.3A 2022-01-18 2022-01-18 Sewage treatment material, preparation method and application Active CN114394678B (en)

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