CN114392355A - Fe3O4@MoS2Preparation method and application of @ SDS composite nano material - Google Patents

Fe3O4@MoS2Preparation method and application of @ SDS composite nano material Download PDF

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CN114392355A
CN114392355A CN202210075726.6A CN202210075726A CN114392355A CN 114392355 A CN114392355 A CN 114392355A CN 202210075726 A CN202210075726 A CN 202210075726A CN 114392355 A CN114392355 A CN 114392355A
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王宏归
王丽婕
张娅
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Abstract

The invention relates to Fe3O4@MoS2Preparation method and application of @ SDS composite nano material, Fe3O4@MoS2The @ SDS composite nano material has a good bacteriostatic effect under infrared induction, has a function of inhibiting drug-resistant gene transmission under the condition of no induction, and has good effects of sterilizing and promoting healing of wound infection. The harm of antibiotics is increasingThe more the field limits or reduces the use of antibiotics, the Fe of the present invention3O4@MoS2The @ SDS composite nano material not only has the functions of inhibiting bacteria and promoting wound healing, but also has the effect of preventing the propagation of drug-resistant genes, so that the @ SDS composite nano material has wide application prospect when being used for treating wound infection, preventing and controlling drug-resistant genes or killing environment.

Description

Fe3O4@MoS2Preparation method and application of @ SDS composite nano material
Technical Field
The invention relates to Fe3O4@MoS2Preparation method and application of @ SDS composite nano material, specifically Fe3O4@MoS2An application technology of a @ SDS composite nano material in infrared induction bacteriostasis and drug-resistant gene propagation inhibition belongs to the field of nano biology.
Background
Infections caused by drug-resistant bacteria pose a serious threat to public health, and therefore, development of novel antibacterial agents having various functions is required. In particular, nanomaterials are one of the promising candidates against the growing crisis of antibiotic resistance. Herein by the reaction of Fe3O4@MoS2The SDS coating thereon synthesizes different Fe3O4@MoS2@ SDS nanocomposite. Photothermal studies show that Fe3O4@MoS2The @ SDS has excellent and stable photothermal properties and can be used as an NIR-induced photothermal reagent. For Escherichia coli (E.coli)E. Coli) Methicillin-resistant staphylococcus aureus and pseudomonas aeruginosa have good disinfection capacity and wound healing capacity in vivo under NIR irradiation. Based on the results of Electron Paramagnetic Resonance (EPR) and free radical trapping experiments, a large amount of superoxide, hydroxyl free radicals, singlet oxygen and living cell reactive oxygen species can be observed under NIR irradiation.
Further, Fe3O4@MoS2The synergistic effect of @ SDS and NIR irradiation almost eliminates MRSA biofilm, a function that enhances Fe3O4@MoS2@ SDS Disinfection Capacity under NIR irradiation. And the inhibitory effect thereof on the spread of antibiotic resistance genes was investigated. Fe as expected3O4@MoS2The @ SDS can effectively and widely block the drug resistance transmission mediated by the sex plasmids, and the blocking effect of the @ SDS is superior to that of Fe3O4@ MoS2 reported by people. In summary, our findings show that Fe3O4@MoS2@ SDS may be a potential candidate for photothermal-photodynamic therapy and inhibition of drug resistance gene transmission.
Disclosure of Invention
The present invention has been made in view of the above problems, and an object of the present invention is to provide Fe3O4@MoS2A preparation method of a @ SDS composite nano material and an application thereof, in particular to an application technology of the composite nano material in photothermal-photodynamic therapy and drug resistance gene propagation inhibition.
The purpose of the invention is realized as follows: fe3O4@MoS2The preparation method of the @ SDS composite nano material is characterized by comprising the following steps of:
(1) 1.50g of FeCl3·6H2O, 2.17g NaAc and 1.00g PEG4000 polyethylene glycol are magnetically stirred in 40mL EG glycol for 1 h; then, the prepared solution was transferred to a 100mL teflon-lined autoclave; continuously reacting for 8 hours at 200 ℃, centrifuging and collecting a product, washing the product with ultrapure water and ethanol until the product is clean, and drying the product in vacuum for 8 hours at 60 ℃ to collect a brown product ferroferric oxide;
(2) for the synthesis of Fe3O4@MoS2A composite material prepared by mixing 0-5.0g of Fe3O460mL of the solution containing 83.3mM TAA thioacetamide and 16.7mM Na was added2MoO4·2H2O, obtaining a mixed solution; subsequently, the mixed solution was transferred into a 100mL autoclave lined with polytetrafluoroethylene and held at 200 ℃ for 20 hours; after naturally cooling to room temperature, the precipitate was collected by centrifugation and washed with ultrapure water and ethanol at least three times; drying the black product at 60 ℃ to obtain Fe-containing product3O4Fe (b) of3O4@MoS2A composite material;
(3) SDS-modified Fe3O4@MoS2Is obtained by the following process:
mixing Fe3O4@MoS2Mixing with SDS in different mass proportions in water, performing ultrasonic treatment for 6h, and washing and drying to obtain Fe in different proportions3O4@MoS2@ SDS (1: n) (n is less than or equal to 10), namely Fe3O4@MoS2@ SDS composite nanomaterial.
Step (3)) In (1), mixing Fe3O4@MoS2Mixed with SDS in water and sonicated for 6 hours, rinsed at least three times with ultrapure water and ethanol, then dried at 60 ℃.
Fe3O4@MoS2The method for applying the @ SDS composite nano material to infrared-induced photothermal effect and bacteriostasis of the material comprises the following specific processes:
a) 0.6 mL of Fe-containing solution was added to the tube3O4@MoS2@ SDS composite nanomaterial solution, then irradiated with infrared, experimental results show: fe3O4@MoS2The @ SDS (1:1) has better thermal effect under the concentration of 120 ug/mL, the photothermal conversion efficiency is as high as 38.65%, and the photothermal conversion efficiency is almost unchanged in the process of repeated use;
b) respectively culturing Escherichia coli, methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa at 37 deg.C overnight with TSB liquid culture medium, respectively collecting thallus, washing with 0.03M PBS for 2-3 times, and suspending with PBS to make thallus concentration to 106CFU/mL; respectively putting 0.6 mL of the suspension of the 3 bacteria into a test tube, and simultaneously adding Fe3O4@MoS2The @ SDS composite nano material is induced and irradiated for 10 minutes by far-red light, and then the Fe under the infrared induction is analyzed by a coating counting method3O4@MoS2The inhibition effect of the @ SDS composite nano material on bacteria.
In the step b), the far-red light induction time is 10 minutes, the photo-thermal conversion efficiency is 38.65%, the repeatability is good, and the bacteriostasis efficiency is high.
Fe3O4@MoS2The method for inhibiting the propagation of drug-resistant genes by using the @ SDS composite nano material comprises the following specific processes:
a) preparation before experiment: a plurality of 15ml glass test tubes, a plurality of 5ml glass tubes, a plurality of 1.5ml centrifuge tubes, a tryptone soy peptone liquid culture medium, a tryptone soy peptone solid culture medium, 0.2 mol PBS and 0.85% normal saline, wherein the whole is sterilized by high pressure steam at 121 ℃ for 15-20 minutes for later use;
b) shaking the bacteria: taking two 15ml glass test tubes, respectively adding 10-12ml tryptone soy peptone liquid culture medium culture solution, respectively inoculating donor bacteria and acceptor bacteria, and culturing at 37 ℃ at 160r/min for 14 hours;
c) collecting donor bacteria and acceptor bacteria in the step b), washing with PBS for 2-3 times to remove residual substances, wherein the residual substances comprise a culture medium and antibiotics, and diluting the bacteria to 5 x 10 by using PBS8 CFU/mL；
d) 1.5ml of the bacterial suspension from step c) are respectively placed in 5ml glass tubes and mixed, and Fe is respectively added into the 5ml glass tubes3O4@MoS2@ SDS composite nanomaterial with no addition of Fe3O4@MoS2The composition of the @ SDS composite was used as a control;
e) the components are placed at 30 ℃ for joint culture, after the culture is finished, a certain volume of bacterial liquid is taken and coated on a screening culture medium, and then the culture is inverted overnight; counting colonies on each plate, calculating the adaptor transformants and the adaptor transfer frequency, and analyzing different doses of Fe3O4@MoS2The effect of the @ SDS composite nanomaterial on the conjugation and transfer of drug-resistant genes.
Fe3O4@MoS2The @ SDS composite nano material has good inhibition effect on conjugative transfer propagation of broad host plasmids and narrow host plasmids.
The method is advanced and scientific, and the Fe provided by the invention3O4@MoS2The preparation method and the application of the @ SDS composite nano material comprise the following steps:
（1）1.50gFeCl3·6H2o, 2.17g NaAc and 1.00g PEG4000 (polyethylene glycol) were magnetically stirred in 40mL EG (ethylene glycol) for 1 h. The prepared solution was then transferred to a 100mL teflon lined autoclave. Continuously reacting for 8 hours at 200 ℃, centrifuging and collecting a product, washing the product with ultrapure water and ethanol until the product is clean, and drying the product in vacuum for 8 hours at 60 ℃ to collect a brown product ferroferric oxide;
(2) for the synthesis of Fe3O4@MoS2Composite material, different amounts of Fe3O4(0-5.0g) 60mL of a solution containing 83.3mM of TAA (thioacetamide) was addedAnd 16.7mM Na2MoO4 ·2H2And (4) in O. Subsequently, each mixed solution was transferred into a 100mL autoclave (Teflon-lined) and maintained at 200 ℃ for 20 hours. After naturally cooling to room temperature, the precipitate was collected by centrifugation and washed with ultrapure water and ethanol at least three times. The black product was dried at 60 ℃. Fe containing n less than or equal to 5g of ferroferric oxide3O4@MoS2Composite material named Fe3O4(ng)@MoS2The experiment adopts Fe3O4(0.5g)@MoS2(Fe according to the invention unless otherwise specified3O4@MoS2Then represents Fe3O4(0.5g)@MoS2）；
(3) Surfactant SDS modified Fe3O4@MoS2Obtained by the following process. Briefly, Fe3O4@MoS2The mass ratio of the nano composite material to SDS is 1:1 mixed in water and then sonicated for 6 h. Washing, drying at 60 ℃ to obtain Fe3O4@MoS2@SDS（1：n）。
Compared with the prior art, the invention has the following beneficial effects:
Fe3O4@MoS2the mixed ultrasonic time with SDS is 6 hours, so that the SDS can be uniformly coated on Fe3O4@MoS2And (5) outside.
Fe3O4@MoS2The @ SDS has excellent photothermal conversion efficiency and infrared induction bacteriostasis efficiency.
Fe3O4@MoS2The @ SDS has a good inhibitory effect not only on broad host plasmids but also on the conjugative transfer spread of narrow host plasmids.
In summary, the invention relates to application of a ferroferric oxide @ molybdenum disulfide @ lauryl sodium sulfate composite nano material in infrared induction bacteriostasis and inhibition of drug-resistant gene transmission. The method comprises the following steps: using FeCl3·6H2O, NaAc and PEG4000 (polyethylene glycol) were magnetically stirred in 40mL EG (ethylene glycol) for 1h, and then the prepared solution was transferred to a Teflon liner at high pressureContinuously reacting for 8 hours at 200 ℃ in a sterilizer, centrifuging to collect the product, washing with ultrapure water and ethanol until the product is clean, vacuum drying for 8 hours at 60 ℃ and collecting brown product ferroferric oxide. For the synthesis of Fe3O4@MoS2Composite material, different amounts of Fe3O460mL of a solution containing 83.3mM TAA (thioacetamide) and 16.7mM Na, respectively, was added2MoO4·2H2And (4) in O. Subsequently, each mixed solution was transferred into a 100mL autoclave and left overnight at 200 ℃. After naturally cooling to room temperature, the precipitate was collected by centrifugation, washed at least three times with ultrapure water and ethanol, and then dried at 60 ℃. Mixing Fe3O4@MoS2Adding into SDS solution, ultrasonic treating for 6 hr, washing, drying, and waiting until Fe3O4@MoS2@ SDS composite nanomaterial.
Using Escherichia coli (E. coli) Drug-resistant Staphylococcus (1)MRSA) Pseudomonas aeruginosa and (B)P. aeruginosa) Testing Fe as a model Strain3O4@MoS2The @ SDS composite nano material has an antibacterial effect under infrared induction. Meanwhile, MRSA T144 is used as an infectious strain in the test, and a rat infected skin wound model is established. Fe was tested using the broad-spectrum host IncP-type plasmid RP4-7 and the narrow-host IncF-type plasmid F33: A-: B-and IncX-type plasmid IncX43O4@MoS2The condition that the @ SDS composite nano material inhibits the diffusion transfer of the drug-resistant gene. The test results show that: fe3O4@MoS2The @ SDS has good infrared induction bacteriostasis capacity, and the Fe3O4@MoS2The @ SDS composite nanomaterial is used for infrared induction bacteriostasis and inhibition of drug-resistant gene conjugation transfer, has the advantages of easiness in recovery, low cost, good performance, low biotoxicity and the like, and has a good application prospect in treatment of water polluted by drug-resistant bacteria and the like and wound infection in the future.
The ferroferric oxide @ molybdenum disulfide @ lauryl sodium sulfate composite nano material has a good antibacterial effect under infrared induction; meanwhile, the compound has the function of inhibiting the transmission of drug-resistant genes under the condition of no induction; has good effects of sterilizing wound infection and promoting healing.
Due to the harm of antibiotics, the use of antibiotics is limited or reduced in more and more fields at present, and the ferroferric oxide @ molybdenum disulfide @ sodium dodecyl sulfate composite nanomaterial disclosed by the invention has the functions of inhibiting bacteria and promoting wound healing and also has the effect of preventing drug-resistant gene transmission, so that the ferroferric oxide @ molybdenum disulfide @ sodium dodecyl sulfate composite nanomaterial has a wide application prospect in wound infection treatment or environmental disinfection.
Drawings
FIG. 1 is a transmission electron microscope image of ferroferric oxide @ molybdenum disulfide in example 1 of the present invention;
FIG. 2 is a transmission electron microscope image of a ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of example 1 in the present invention;
FIG. 3 shows the photothermal conversion efficiency of the ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of example 2 of the present invention;
fig. 4 shows the bacteriostasis effect of the ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of embodiment 2 under infrared induction.
Fig. 5 shows the effect of the ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of embodiment 3 of the present invention on inhibiting the propagation of drug-resistant gene conjugation.
Detailed Description
The invention will be described in further detail with reference to the following drawings and detailed description, which are provided for reference and illustration purposes only and are not intended to limit the invention.
Example 1
（1）1.50gFeCl3·6H2O, 2.17g NaAc and 1.00g PEG4000 (polyethylene glycol) were magnetically stirred in 40mL EG (ethylene glycol) for 1 h. The prepared solution was then transferred to a 100mL teflon lined autoclave. Continuously reacting for 8 hours at 200 ℃, centrifuging and collecting a product, washing the product with ultrapure water and ethanol until the product is clean, and drying the product in vacuum for 8 hours at 60 ℃ to collect a brown product ferroferric oxide;
(2) for the synthesis of Fe3O4@MoS2Composite material, 0.5g Fe3O4Add 60mL of solutionSolutions containing 83.3mM TAA (thioacetamide) and 16.7mM Na, respectively2MoO4 ·2H2And (4) in O. Subsequently, each mixed solution was transferred into a 100mL autoclave (Teflon-lined) and maintained at 200 ℃ for 20 hours. Naturally cooling to room temperature, centrifuging to collect precipitate, washing with ultrapure water and ethanol for at least three times, and drying at 60 deg.C to obtain Fe of ferroferric oxide3O4@MoS2A composite material;
(3) surfactant SDS modified Fe3O4@MoS2Obtained by the following process. Briefly, Fe3O4@MoS2Mixing the nano composite material and SDS in water according to the mass ratio of 1:1, then carrying out ultrasonic treatment for 6h, washing, and drying at 60 ℃ for 12 h to obtain Fe3O4@MoS2@SDS(1:1)；
FIG. 1 is a transmission electron microscope image of ferroferric oxide @ molybdenum disulfide in example 1 of the present invention; FIG. 2 is a transmission electron microscope image of a ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of example 1 in the present invention;
example 2
The ferroferric oxide-molybdenum disulfide-lauryl sodium sulfate composite nano material obtained in the embodiment 1 is used for infrared induction photothermal effect and bacteriostatic effect thereof, and the specific process is as follows:
a) 0.6 mL of the nanomaterial-containing solution was added to the tube and then irradiated with infrared, and the experimental results showed that: fe3O4@MoS2The @ SDS (1:1) has better thermal effect under the concentration of 120 ug/mL, the photothermal conversion efficiency is as high as 38.65% (figure 3), and the photothermal conversion efficiency is almost unchanged in the process of repeated use;
b) respectively culturing Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa at 37 deg.C overnight with TSB liquid culture medium, respectively collecting thallus, washing with 0.03M PBS for 2-3 times, and suspending with PBS to obtain thallus concentration of 106CFU/mL. 0.6 mL of the suspension of the 3 bacteria is added into a test tube, the nano material is added into the test tube, and then the test tube is induced and irradiated by 0.7W of far-red light for 10 minutes, and the experimental result shows that: fe3O4@MoS2The bactericidal efficiency of @ SDS (1:1) on large intestine, MRSA and Pseudomonas aeruginosa under infrared induction was 99.6%, 99.8% and 98.8%, respectively (FIG. 4).
FIG. 3 shows the photothermal conversion efficiency of the ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of example 2 of the present invention; fig. 4 shows the bacteriostasis effect of the ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of embodiment 2 under infrared induction.
Example 3
The ferroferric oxide-molybdenum disulfide-lauryl sodium sulfate composite nano material obtained in the example 1 is used for inhibiting the gene junction transfer performance, and the specific process is as follows:
a) preparation before experiment: several 15ml glass test tubes, several 5ml glass tubes, several 1.5ml centrifuge tubes, a tryptone soy peptone liquid medium, a tryptone soy peptone solid medium, 0.2 mol PBS, 0.85% normal saline, all of which are sterilized by high pressure steam at 121 ℃ for 15-20 minutes for later use.
b) Shaking the bacteria: two 15ml glass test tubes are taken, 10-12ml tryptone soy peptone liquid medium culture solution is respectively added, then donor bacteria and acceptor bacteria are respectively inoculated, and the culture is carried out for 14 hours at 37 ℃ and at 160 r/min.
c) Collecting donor bacteria and acceptor bacteria in the step b), washing with PBS for 2-3 times to remove residual culture medium, antibiotics and the like, and diluting the bacteria to 5 x 10 by using PBS8 CFU/mL；
d) Respectively taking 1.5ml of the bacterial suspension in c) into a 5ml glass test tube and uniformly mixing, respectively adding 100 mg/L of nano material into the glass test tube, and taking the component without the material as a reference;
e) the components are placed at 30 ℃ for joint culture, after the culture is finished, a certain volume of bacterial liquid is taken and coated on a screening culture medium, and then the culture is carried out in an inverted mode overnight. The colony number on each plate is counted, and the joint transformant and the joint transfer frequency are calculated, and the results show that: fe3O4@MoS2The @ SDS (1:1) composite nano material has good inhibition effect on the drug-resistant gene conjugation transfer propagation mediated by broad host and narrow host plasmids, and can be used for inhibiting broad hostThe fold inhibition of conjugative transfer propagation of the sex plasmid RP4-7 and the narrow host plasmid IncF type plasmid F33: A-: B-and the IncX type plasmid IncX4 were: 66-, 8.5-, 56-fold (FIG. 5).
Fig. 5 shows the effect of the ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate composite nanomaterial of embodiment 3 of the present invention on inhibiting the propagation of drug-resistant gene conjugation.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention. In addition to the above embodiments, the present invention may have other embodiments, for example, the amount of the composite nanomaterial and the sampling and plating time may be appropriately enlarged. All technical solutions formed by adopting equivalent substitutions or equivalent transformations fall within the protection scope of the claims of the present invention. Technical features of the present invention which are not described may be implemented by or using the prior art, and will not be described herein.

Claims (6)

1. Fe3O4@MoS2The preparation method of the @ SDS composite nano material is characterized by comprising the following steps of:
(1) 1.50g of FeCl3·6H2O, 2.17g NaAc and 1.00g PEG4000 polyethylene glycol are magnetically stirred in 40mL EG glycol for 1 h; then, the prepared solution was transferred to a 100mL teflon-lined autoclave; continuously reacting for 8 hours at 200 ℃, centrifuging and collecting a product, washing the product with ultrapure water and ethanol until the product is clean, and drying the product in vacuum for 8 hours at 60 ℃ to collect a brown product ferroferric oxide;
(2) for the synthesis of Fe3O4@MoS2A composite material prepared by mixing 0-5.0g of Fe3O460mL of the solution containing 83.3mM TAA thioacetamide and 16.7mM Na was added2MoO4·2H2O, obtaining a mixed solution; subsequently, the mixed solution was transferred into a 100mL autoclave lined with polytetrafluoroethylene and held at 200 ℃ for 20 hours; after naturally cooling to room temperature, the precipitate was collected by centrifugation and washed with ultrapure water and ethanol at least three times; drying the black product at 60 ℃ to obtain Fe-containing product3O4Fe (b) of3O4@MoS2A composite material;
(3) SDS-modified Fe3O4@MoS2Is obtained by the following process:
mixing Fe3O4@MoS2Mixing with SDS in different mass proportions in water, performing ultrasonic treatment for 6h, and washing and drying to obtain Fe in different proportions3O4@MoS2@ SDS (1: n) (n is less than or equal to 10), namely Fe3O4@MoS2@ SDS composite nanomaterial.
2. Fe of claim 13O4@MoS2The preparation method of the @ SDS composite nano material is characterized in that in the step (3), Fe3O4@MoS2Mixed with SDS in water and sonicated for 6 hours, rinsed at least three times with ultrapure water and ethanol, then dried at 60 ℃.
3. An Fe element according to any one of claims 1-23O4@MoS2The method for applying the @ SDS composite nano material to infrared-induced photothermal effect and bacteriostasis is characterized by comprising the following specific processes:
a) 0.6 mL of Fe-containing solution was added to the tube3O4@MoS2@ SDS composite nanomaterial solution, then irradiated with infrared, experimental results show: fe3O4@MoS2The @ SDS (1:1) has better thermal effect under the concentration of 120 ug/mL, the photothermal conversion efficiency is as high as 38.65%, and the photothermal conversion efficiency is almost unchanged in the process of repeated use;
b) respectively culturing Escherichia coli, methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa at 37 deg.C overnight with TSB liquid culture medium, respectively collecting thallus, washing with 0.03M PBS for 2-3 times, and suspending with PBS to make thallus concentration to 106CFU/mL; respectively putting 0.6 mL of the suspension of the 3 bacteria into a test tube, and simultaneously adding Fe3O4@MoS2The @ SDS composite nanomaterial is induced to irradiate for 10 min by far-red light and then utilizedAnalysis of Fe under infrared induction by plate coating counting method3O4@MoS2The inhibition effect of the @ SDS composite nano material on bacteria.
4. The method as claimed in claim 3, wherein in the step b), the far-red light induction time is 10 minutes, the photothermal conversion efficiency is 38.65%, the repeatability is good, and the bacteriostatic efficiency is high.
5. An Fe alloy according to claim 13O4@MoS2A method for inhibiting drug-resistant gene transmission by using a @ SDS composite nano material is characterized by comprising the following specific steps of:
a) preparation before experiment: a plurality of 15ml glass test tubes, a plurality of 5ml glass tubes, a plurality of 1.5ml centrifuge tubes, a tryptone soy peptone liquid culture medium, a tryptone soy peptone solid culture medium, 0.2 mol PBS and 0.85% normal saline, wherein the whole is sterilized by high pressure steam at 121 ℃ for 15-20 minutes for later use;
b) shaking the bacteria: taking two 15ml glass test tubes, respectively adding 10-12ml tryptone soy peptone liquid culture medium culture solution, respectively inoculating donor bacteria and acceptor bacteria, and culturing at 37 ℃ at 160r/min for 14 hours;
c) collecting donor bacteria and acceptor bacteria in the step b), washing with PBS for 2-3 times to remove residual substances, wherein the residual substances comprise a culture medium and antibiotics, and diluting the bacteria to 5 x 10 by using PBS8 CFU/mL；
d) 1.5ml of the bacterial suspension from step c) are respectively placed in 5ml glass tubes and mixed, and Fe is respectively added into the 5ml glass tubes3O4@MoS2@ SDS composite nanomaterial with no addition of Fe3O4@MoS2The composition of the @ SDS composite was used as a control;
e) the components are placed at 30 ℃ for joint culture, after the culture is finished, a certain volume of bacterial liquid is taken and coated on a screening culture medium, and then the culture is inverted overnight; counting colonies on each plate, calculating the adaptor transformants and the adaptor transfer frequency, and analyzing different doses of Fe3O4@MoS2@ SDS complexationThe effect of the nano material on the conjugation and transfer of drug-resistant genes.
6. The method of claim 5, wherein the Fe is3O4@MoS2The @ SDS composite nano material has good inhibition effect on conjugative transfer propagation of broad host plasmids and narrow host plasmids.
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CN115709098A (en) * 2022-11-30 2023-02-24 扬州大学 Synthetic method and application of ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate nano composite

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HONGGUI WANG等: "Fe3O4 composited with MoS2 blocks horizontal gene transfer", COLLOIDS AND SURFACES B: BIOINTERFACES *

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115709098A (en) * 2022-11-30 2023-02-24 扬州大学 Synthetic method and application of ferroferric oxide-molybdenum disulfide-sodium dodecyl sulfate nano composite

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