CN114392288A - Method for extracting effective components from chenopodium ambrosioides - Google Patents
Method for extracting effective components from chenopodium ambrosioides Download PDFInfo
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- CN114392288A CN114392288A CN202210132705.3A CN202210132705A CN114392288A CN 114392288 A CN114392288 A CN 114392288A CN 202210132705 A CN202210132705 A CN 202210132705A CN 114392288 A CN114392288 A CN 114392288A
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- chenopodium ambrosioides
- chenopodium
- ambrosioides
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- 241000309574 Dysphania ambrosioides Species 0.000 title claims abstract description 49
- 235000000509 Chenopodium ambrosioides Nutrition 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000000706 filtrate Substances 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 11
- 239000012535 impurity Substances 0.000 claims abstract description 6
- 229940020871 chenopodium ambrosioides extract Drugs 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000001694 spray drying Methods 0.000 claims abstract description 4
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 239000013566 allergen Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000001556 precipitation Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 17
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 201000002045 Ancylostomiasis Diseases 0.000 description 1
- 208000033211 Ankylostomiasis Diseases 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010020376 Hookworm infection Diseases 0.000 description 1
- 201000009361 ascariasis Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 210000001099 axilla Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 206010014881 enterobiasis Diseases 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses an extraction method for extracting effective components from chenopodium ambrosioides, which comprises the following steps: treating fresh chenopodium ambrosioides, and mixing the chenopodium ambrosioides obtained by pretreatment with an alcohol solution according to the proportion of 1: 20, extracting at 65 ℃ for 5 times, each time for 30-45 minutes, combining the filtrates filtered after the 5 times of extraction, and cooling at room temperature; coarsely filtering the cooled chenopodium ambrosioides filtrate, and then precisely separating to obtain the precision filtrate of chenopodium ambrosioides; adding the precise filtrate into an ultrasonic extractor to obtain an ultrasonic treatment solution; and (3) carrying out microwave vacuum low-temperature drying, vacuum freeze drying or spray drying on the ultrasonic treatment liquid to finally obtain the chenopodium ambrosioides extract. In the method for extracting the chenopodium ambrosioides, the impurities of the allergen are effectively reduced by coarse crushing, precipitation and filtration for multiple times, the filtration effect is improved by multiple filtration modes, the method has simple process, is beneficial to industrialized production, and has large extraction amount.
Description
Technical Field
The invention relates to the technical field of plant extraction, in particular to an extraction method for extracting effective components from chenopodium ambrosioides.
Background
Chenopodium ambrosioides is an annual or perennial herb, 50-80 cm high, and has strong fragrance. The stem is upright, and is multi-branched, and the stem has colored strips and blunt edges; branches are usually thin. The blade is rectangular and round, and is in the shape of a needle, the tip of the blade is sharp or tapered, and the edge of the blade is provided with sparse and irregular large saw teeth. Flowers are both sexually and female, usually 3-5 clusters, and originate in the upper axilla. The cygon is oblate and completely covered in the flower quilt. The seeds are transverse or oblique, black or dark red, smooth and glossy. The time of the flowering phase and the fruit phase is long. The whole herb is used as a medicine for treating ascariasis, ancylostomiasis and enterobiasis, and externally used for treating skin eczema and maggot.
Disclosure of Invention
Aiming at the problems, the invention provides an extraction method for extracting effective components from chenopodium ambrosioides, aims to solve the defects in the prior production technology, and provides a method for extracting the effective components from chenopodium ambrosioides.
According to one object of the invention, the invention provides an extraction method for extracting effective components from chenopodium ambrosioides, which comprises the following steps:
s1, processing fresh chenopodium ambrosioides
Removing impurities on the surface of fresh chenopodium ambrosioides, and pulverizing chenopodium ambrosioides at low temperature of-10 ℃ to obtain chenopodium ambrosioides with fineness below 8 meshes;
s2, mixing the pre-treated chenopodium ambrosioides with an alcohol solution according to the ratio of 1: 20, extracting at 65 ℃ for 5 times, each time for 30-45 minutes, combining the filtrates filtered after the 5 times of extraction, and cooling at room temperature;
s3, performing precision separation after coarse filtration of the cooled chenopodium ambrosioides filtrate, and filtering through a precision membrane with the aperture of 3-10um to obtain precision filtrate of chenopodium ambrosioides;
s4, adding the precision filtrate into an ultrasonic extractor to obtain an ultrasonic treatment solution;
s5, performing microwave vacuum low-temperature drying, vacuum freeze drying or spray drying on the ultrasonic treatment solution to obtain the chenopodium ambrosioides extract.
Further, the fresh chenopodium ambrosioides is chenopodium ambrosioides plants harvested within 3 days or chenopodium ambrosioides plants which are not putrefied after fresh-keeping treatment.
Further, the alcohol solution is an aqueous solution of methanol or ethanol.
Further, in S1, the rotational speed of the pulverizer in the low-temperature pulverization step is 3800 r/min.
Further, the pH value of the solution is adjusted by adding fruit acid before adding the fruit acid into the ultrasonic extractor, so that the pH value of the mixture solution is between 3 and 4.
Further, the ultrasonic extractor is subjected to ultrasonic treatment for 2.5 hours and 2.5 hours or more at the temperature of 50-60 ℃ under the condition of 60-80 kHZ.
The invention has the beneficial effects that:
in the method for extracting the effective components of the chenopodium ambrosioides, the impurities of the allergen are effectively reduced by coarse crushing, precipitation and filtration for multiple times, the filtration effect is improved by multiple filtration modes, the method has simple process, is beneficial to industrialized production, and has large extraction amount.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for extracting effective components from Chenopodium ambrosioides comprises the following steps:
s1, processing fresh chenopodium ambrosioides
The fresh chenopodium ambrosioides is chenopodium ambrosioides plants harvested within 3 days or chenopodium ambrosioides plants which are not rotten after fresh-keeping treatment. Removing impurities on the surface of fresh chenopodium ambrosioides, and pulverizing chenopodium ambrosioides at low temperature of-10 ℃ to obtain chenopodium ambrosioides with fineness below 8 meshes; the rotation speed of the pulverizer in the low-temperature pulverizing step is 3800 r/min.
S2, mixing the pre-treated chenopodium ambrosioides with an alcohol solution (the alcohol solution is methanol or ethanol aqueous solution) according to the ratio of 1: 20, extracting at 65 ℃ for 5 times, each time for 30-45 minutes, combining the filtrates filtered after the 5 times of extraction, and cooling at room temperature;
s3, performing precision separation after coarse filtration of the cooled chenopodium ambrosioides filtrate, and filtering through a precision membrane with the aperture of 3-10um to obtain precision filtrate of chenopodium ambrosioides;
s4, adding the precision filtrate into an ultrasonic extractor to obtain an ultrasonic treatment solution; the pH of the solution is adjusted by adding fruit acid before adding into the ultrasonic extractor, so that the pH of the mixture solution is between 3 and 4. Ultrasonic treatment is carried out for 2.5 hours and more than 2.5 hours under the conditions of 60-80kHZ and 50-60 ℃.
S5, performing microwave vacuum low-temperature drying, vacuum freeze drying or spray drying on the ultrasonic treatment solution to obtain the chenopodium ambrosioides extract.
According to the extraction method of the effective components of the chenopodium ambrosioides, the impurities of the allergen are effectively reduced through multiple times of coarse crushing, precipitation and filtration, the filtration effect is improved through multiple filtration modes, the method is simple in process, beneficial to industrial production, large in extraction amount and capable of meeting the requirements of the chenopodium ambrosioides.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (6)
1. The extraction method for extracting the effective components in the chenopodium ambrosioides is characterized by comprising the following steps:
s1, processing fresh chenopodium ambrosioides
Removing impurities on the surface of fresh chenopodium ambrosioides, and pulverizing chenopodium ambrosioides at low temperature of-10 ℃ to obtain chenopodium ambrosioides with fineness below 8 meshes;
s2, mixing the pre-treated chenopodium ambrosioides with an alcohol solution according to the ratio of 1: 20, extracting at 65 ℃ for 5 times, each time for 30-45 minutes, combining the filtrates filtered after the 5 times of extraction, and cooling at room temperature;
s3, performing precision separation after coarse filtration of the cooled chenopodium ambrosioides filtrate, and filtering through a precision membrane with the aperture of 3-10um to obtain precision filtrate of chenopodium ambrosioides;
s4, adding the precision filtrate into an ultrasonic extractor to obtain an ultrasonic treatment solution;
s5, performing microwave vacuum low-temperature drying, vacuum freeze drying or spray drying on the ultrasonic treatment solution to obtain the chenopodium ambrosioides extract.
2. The method for extracting effective components from Chenopodium ambrosioides according to claim 1, wherein the fresh Chenopodium ambrosioides is Chenopodium ambrosioides harvested within 3 days or Chenopodium ambrosioides without putrefaction after freshness retaining treatment.
3. The method of claim 1, wherein the alcohol solution is methanol or ethanol.
4. The method of claim 1, wherein the rotation speed of the pulverizer in the low-temperature pulverization step is 3800r/min in S1.
5. The method of claim 1, wherein the pH of the solution is adjusted by adding fruit acid to the solution to a pH of between 3 and 4 prior to the ultrasonic extractor.
6. The method of claim 1, wherein the ultrasonic extractor is used for ultrasonic treatment at 50-60 deg.C under 60-80kHZ for 2.5 hours or more and 2.5 hours or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210132705.3A CN114392288A (en) | 2022-02-14 | 2022-02-14 | Method for extracting effective components from chenopodium ambrosioides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210132705.3A CN114392288A (en) | 2022-02-14 | 2022-02-14 | Method for extracting effective components from chenopodium ambrosioides |
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| Publication Number | Publication Date |
|---|---|
| CN114392288A true CN114392288A (en) | 2022-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210132705.3A Pending CN114392288A (en) | 2022-02-14 | 2022-02-14 | Method for extracting effective components from chenopodium ambrosioides |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1990006A (en) * | 2005-12-31 | 2007-07-04 | 天津天士力制药股份有限公司 | Chenopodium ambrosioides extract, preparation thereof, preparation method and use thereof |
| CN102532155A (en) * | 2010-12-24 | 2012-07-04 | 苏州宝泽堂医药科技有限公司 | Method for extracting ascaridol and alpha-spinasterol from wormseed |
| CN104887725A (en) * | 2015-05-22 | 2015-09-09 | 昆明理工大学 | Novel application of chenopodium ambrosioides whole-plant extract |
-
2022
- 2022-02-14 CN CN202210132705.3A patent/CN114392288A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1990006A (en) * | 2005-12-31 | 2007-07-04 | 天津天士力制药股份有限公司 | Chenopodium ambrosioides extract, preparation thereof, preparation method and use thereof |
| CN102532155A (en) * | 2010-12-24 | 2012-07-04 | 苏州宝泽堂医药科技有限公司 | Method for extracting ascaridol and alpha-spinasterol from wormseed |
| CN104887725A (en) * | 2015-05-22 | 2015-09-09 | 昆明理工大学 | Novel application of chenopodium ambrosioides whole-plant extract |
Non-Patent Citations (2)
| Title |
|---|
| 朱晓换，等: "土荆芥醇提物对MCF-7细胞增殖的影响", 《四川师范大学学报(自然科学版)》 * |
| 黄九玲，等: "土荆芥提取物对谷蠹和赤拟谷盗的触杀与熏蒸活性研究", 《中国粮油学报》 * |
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Application publication date: 20220426 |
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