CN114380292A - Preparation method and application of magnesium silicide two-dimensional nanosheet, and preparation method and application of hydrogen-releasing hydrogel wound dressing - Google Patents
Preparation method and application of magnesium silicide two-dimensional nanosheet, and preparation method and application of hydrogen-releasing hydrogel wound dressing Download PDFInfo
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- CN114380292A CN114380292A CN202210076555.9A CN202210076555A CN114380292A CN 114380292 A CN114380292 A CN 114380292A CN 202210076555 A CN202210076555 A CN 202210076555A CN 114380292 A CN114380292 A CN 114380292A
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- magnesium silicide
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- hydrogen
- hydrogel
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- YTHCQFKNFVSQBC-UHFFFAOYSA-N magnesium silicide Chemical compound [Mg]=[Si]=[Mg] YTHCQFKNFVSQBC-UHFFFAOYSA-N 0.000 title claims abstract description 213
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- 239000000017 hydrogel Substances 0.000 title claims abstract description 81
- 239000001257 hydrogen Substances 0.000 title claims abstract description 81
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- 238000002360 preparation method Methods 0.000 title claims abstract description 55
- 206010052428 Wound Diseases 0.000 claims abstract description 78
- 208000027418 Wounds and injury Diseases 0.000 claims abstract description 78
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- DGVMNQYBHPSIJS-UHFFFAOYSA-N dimagnesium;2,2,6,6-tetraoxido-1,3,5,7-tetraoxa-2,4,6-trisilaspiro[3.3]heptane;hydrate Chemical compound O.[Mg+2].[Mg+2].O1[Si]([O-])([O-])O[Si]21O[Si]([O-])([O-])O2 DGVMNQYBHPSIJS-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention discloses a preparation method and application of a magnesium silicide two-dimensional nanosheet, and a preparation method and application of a hydrogen release hydrogel wound dressing, wherein the preparation method of the magnesium silicide two-dimensional nanosheet comprises the following steps: adding magnesium silicide raw powder into an alcohol solvent, stirring and mixing to obtain a mixture A; and (3) carrying out ultrasonic stripping on the mixture A to obtain the magnesium silicide two-dimensional nanosheet. The synthesis process does not need strict control of environmental temperature and the like, is simple in preparation process, easily available in raw materials and low in cost. The prepared magnesium silicide two-dimensional nano-sheet has higher activity, can carry out long-acting hydrogen slow release under physiological conditions, can be used as a main raw material of a hydrogen release dressing, and can be used for repairing and regenerating large-area burn wounds.
Description
Technical Field
The invention relates to the technical field of preparation of magnesium silicide nanosheets and the technical field of medical treatment, in particular to a preparation method and application of a magnesium silicide two-dimensional nanosheet and a preparation method and application of a hydrogen-releasing hydrogel wound dressing.
Background
Due to magnesium silicide (molecular formula is Mg)2Si) is silicon and magnesium, which are among the most abundant elements on earth, and thus are non-toxic bio-safe materials. Meanwhile, the silicon nitride has the advantages of narrow band gap, good light absorption coefficient, good hot spot mechanical property, good photoelectric conversion performance and the like, so that the silicon nitride is generally used for preparing a semiconductor or a light solar cell.
Currently, in the market, silicon powder and magnesium powder are generally synthesized into fine magnesium silicide powder by ball milling or calcining under the protection of inert gas. However, these methods require extremely high temperatures (>500 ℃), are complicated to prepare and costly to produce, and most of the produced powders are micron-sized particles.
Disclosure of Invention
The invention mainly aims to provide a preparation method and application of a magnesium silicide two-dimensional nanosheet, and a preparation method and application of a hydrogen release hydrogel wound dressing, and aims to simplify the preparation of the magnesium silicide two-dimensional nanosheet and save the generation cost.
In order to achieve the purpose, the invention provides a preparation method of a magnesium silicide two-dimensional nanosheet, which comprises the following steps:
s10, adding the magnesium silicide raw powder into an alcohol solvent, stirring and mixing to obtain a mixture A;
s20, carrying out ultrasonic stripping on the mixture A to obtain the magnesium silicide two-dimensional nanosheet.
Optionally, in step S10, the purity of the magnesium silicide raw powder is greater than or equal to 99%; and/or the presence of a gas in the gas,
the alcohol solvent comprises at least one of acetone, glycol, ethanol and glycerol; and/or adding 1 mg-5 g of the magnesium silicide raw powder correspondingly to each 1mL of the alcohol solvent; and/or, in step S20, ultrasonic exfoliation is performed by a sonicator.
Optionally, the power of the ultrasonic crusher is 100-2The time of ultrasonic crushing is 5 to100min。
Optionally, after step S20, the method further includes:
and S30, adding a modifier into the magnesium silicide two-dimensional nanosheet, centrifuging and washing to obtain the modified magnesium silicide two-dimensional nanosheet.
Optionally, in step S30, the modifier has a purity of 99% or more and a molecular weight of 40000 to 70000 daltons.
Optionally, the modifier comprises one of polyvinylpyrrolidone, polylactic acid, and polyethylene glycol.
In order to realize the purpose, the invention provides a preparation method of a hydrogen-releasing hydrogel wound dressing, which comprises the following steps:
s100, preparing a hydrogel substrate;
s200, adding the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet into the hydrogel substrate, and mixing uniformly to prepare the hydrogel wound dressing.
Optionally, 1-500 mL of hydrogel substrate is correspondingly added to each 1 mug of the magnesium silicide two-dimensional nanosheet; and/or the presence of a gas in the gas,
the step S100 includes:
s1001, providing a chitosan solution with the mass concentration of 0.5-2%, a sodium glycerophosphate solution with the mass concentration of 7-12% and a sodium hyaluronate solution with the mass concentration of 0.2-1%;
s1002, mixing the chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution according to the weight ratio of 10-60: 30: and (3) uniformly mixing the components in a volume ratio of 10-60 to prepare the hydrogel substrate.
In order to achieve the purpose, the invention provides an application of the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet in preparing burn dressings.
Optionally, the application in preparing the material for promoting the expression of the vascular endothelial cell growth factor; or the like, or, alternatively,
the application in preparing the material for promoting the angiogenesis of deep burn wound surfaces.
In order to achieve the purpose, the invention provides an application of the hydrogel wound dressing prepared by the preparation method of the hydrogel wound dressing in preparation of a medical device for repairing deep burn wounds; or the like, or, alternatively,
the application in preparing medical device for promoting the vascular endothelial growth factor expression in burn wound; or the like, or, alternatively,
the application in preparing medical devices for promoting angiogenesis of deep burn wounds and/or constructing immune microenvironment for promoting tissue repair.
The invention provides a preparation method of a magnesium silicide two-dimensional nanosheet, which comprises the steps of adding raw magnesium silicide powder into an alcohol solvent, stirring and mixing to obtain a mixture A, and carrying out ultrasonic stripping on the mixture A to obtain the magnesium silicide two-dimensional nanosheet. The prepared magnesium silicide two-dimensional nano-sheet has higher activity, can carry out long-acting hydrogen slow release under physiological conditions, can be used as a main raw material of a hydrogen release dressing, and can be used for repairing and regenerating large-area burn wounds.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other related drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of an embodiment of a preparation method of a magnesium silicide two-dimensional nanosheet provided by the present invention;
FIG. 2 is a schematic structural diagram of one embodiment of a method for preparing a hydrogen-releasing hydrogel wound dressing provided by the present invention;
FIG. 3 is an FT-IR spectrum of magnesium silicide nanoplates prepared by the method of preparing the magnesium silicide two-dimensional nanoplates provided in FIG. 1;
FIG. 4 is an XRD spectrum of a magnesium silicide nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet provided in FIG. 1;
fig. 5 is a Transmission Electron Microscope (TEM) characterization map of the magnesium silicide nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet provided in fig. 1.
FIG. 6 is an Atomic Force Microscope (AFM) characterization map of a two-dimensional nanoplatelet of magnesium silicide;
FIG. 7 is a statistical analysis diagram corresponding to an Atomic Force Microscope (AFM) characterization spectrum of a two-dimensional magnesium silicide nanosheet;
FIG. 8 is a graph showing the change of hydrogen release of two-dimensional magnesium silicide nanosheets in PBS solutions at different pH values;
FIG. 9 is a graph of hydrogen evolution of two-dimensional nano-sheets of magnesium silicide in hydrogel at different pH values;
FIG. 10 is a schematic diagram showing the effect of magnesium silicide two-dimensional nanoplates on the proliferation capacity of HaCat cells;
fig. 11 is a schematic diagram of the effect of magnesium silicide two-dimensional nanoplatelets on HSF cell proliferation capacity;
FIG. 12 is a schematic illustration of the effect of magnesium silicide two-dimensional nanoplates on the proliferative capacity of HMEC-1 cells;
FIG. 13 is a schematic diagram showing the effect of magnesium silicide two-dimensional nanoplates on HaCat cell migration ability;
FIG. 14 is a graph of statistics of migration distances of HaCat cells of FIG. 13;
FIG. 15 is a schematic diagram showing the effect of magnesium silicide two-dimensional nanosheets on HaCat cell migration ability;
FIG. 16 is a graph of HSF cell migration distance statistics of FIG. 15;
FIG. 17 is a schematic diagram showing the effect of magnesium silicide two-dimensional nanoplates on HaCat cell migration ability;
FIG. 18 is a graph of the migration distance statistics for HMEC-1 cells of FIG. 17;
fig. 19 is an optical observation view of the formation of blood vessels under the condition of hydrogen gas released from the magnesium silicide two-dimensional nanosheets;
FIG. 20 is a statistical plot of the number of vessels corresponding to FIG. 19;
figure 21 is a digital photograph of the wound healing process on the back of mice on days 7 and 14 of wound treatment by hydrogen-releasing hydrogel dressing;
FIG. 22 is a graph of a statistical analysis of the dynamic changes in wound area on the back of the mouse in FIG. 21;
FIG. 23 is a pathological analysis of the back wound (HE staining) in mice of different experimental groups;
FIG. 24 is a pathological analysis of the back wound (Masson staining) of mice from different experimental groups;
FIG. 25 is a graph of statistical analysis of the thickness of the dermal layer of the dorsal wound of mice in different experimental groups;
FIG. 26 is a graph of statistical analysis of the thickness of the skin across the wound on the back of mice from different experimental groups;
fig. 27 is a schematic view of immunofluorescence staining effects of CD31 and α -SMA on a wound site of a hydrogen-releasing hydrogel wound dressing after 7 days of treatment of a deep ii-degree burn wound;
FIG. 28 is a graph of fluorescence intensity statistics for CD31 and α -SMA expression in FIG. 27;
FIG. 29 is a graph showing the immunofluorescence staining effect of CD31 and α -SMA on a wound site after 14 days of treatment;
FIG. 30 is a graph of the distribution of mean diameter and number of blood vessels at the wound site after 14 days of treatment;
FIG. 31 is a schematic view showing analysis of expression of VEGF and CD31 in burn wounds on day 7 of deep II degree burn wound repair by Western blot;
FIG. 32 is a schematic view showing analysis of expression of VEGF and CD31 in burn wounds at day 14 of deep II degree burn wound repair by Western blot;
FIG. 33 is a statistical chart of analysis of VEGF expression in burn wounds during deep II degree burn wound repair by Western blot;
FIG. 34 is a statistical chart of analysis of the expression of CD31 in burn wounds during deep II-degree burn wound repair by Western blot;
fig. 35 is a schematic diagram of a synthetic route of a hydrogen-releasing hydrogel wound dressing.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B" including either A or B or both A and B. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Since the main components of magnesium silicide are silicon and magnesium, which are one of the most abundant elements on earth, it is a nontoxic, biologically safe material. Meanwhile, the silicon nitride has the advantages of narrow band gap, good light absorption coefficient, good hot spot mechanical property, good photoelectric conversion performance and the like, so that the silicon nitride is generally used for preparing a semiconductor or a light solar cell.
Currently, in the market, silicon powder and magnesium powder are generally synthesized into fine magnesium silicide powder by ball milling or calcining under the protection of inert gas. However, these methods require extremely high temperatures (>500 ℃), are complicated to prepare and costly to produce, and most of the produced powders are micron-sized particles.
Based on the above concept, the present invention provides a method for preparing a magnesium silicide two-dimensional nanosheet, and fig. 1 shows an embodiment of the method for preparing a magnesium silicide two-dimensional nanosheet provided by the present invention. Referring to fig. 1, in the present embodiment, the preparation method of the magnesium silicide two-dimensional nanosheet includes the following steps:
s10, adding the magnesium silicide raw powder into an alcohol solvent, stirring and mixing to obtain a mixture A;
in step S10, the purity of the magnesium silicide raw powder is greater than or equal to 99%, which greatly increases the content of magnesium silicide in the magnesium silicide raw powder, so that a large amount of hydrogen can be generated when magnesium silicide is hydrolyzed.
In one embodiment, the particle size of the magnesium silicide raw powder is 5-150 μm, so that the magnesium silicide raw powder can be fully contacted with the alcohol solvent, and the quality of the prepared magnesium silicide two-dimensional nanosheet is improved.
In one embodiment, the particle size of the magnesium silicide two-dimensional nanosheet is about 700nm, and the thickness of the magnesium silicide two-dimensional nanosheet is about 0.8nm, so that the magnesium silicide two-dimensional nanosheet can be subjected to hydrolysis reaction conveniently, and the efficiency of hydrogen release through hydrolysis is improved.
In one embodiment, the alcoholic solvent includes at least one of acetone, ethylene glycol, ethanol, and glycerol.
In one embodiment, the purity of the alcohol solvent is greater than or equal to 99%, so that the hydrolysis reaction of the magnesium silicide raw powder is avoided.
In one embodiment, 1mg to 5g of the magnesium silicide raw powder is added to 1mL of the alcohol solvent, so that the magnesium silicide raw powder and the alcohol solvent can be uniformly mixed.
It should be noted that, when the above embodiments are satisfied at the same time, the impurities in the magnesium silicide two-dimensional nanosheets can be reduced, and the efficiency of releasing hydrogen can be improved.
S20, carrying out ultrasonic stripping on the mixture A to obtain the magnesium silicide two-dimensional nanosheet.
In step S20, ultrasonic stripping is performed by an ultrasonic crusher, and thus, the alcohol solvent generates cavitation by using the dispersion effect of ultrasonic waves in the alcohol solvent, so that the magnesium silicide raw powder in the alcohol solvent is crushed, thereby obtaining the magnesium silicide two-dimensional nanosheet.
Specifically, the power of the ultrasonic crusher is 100-1000, and the time of ultrasonic crushing is 5-100 min, so that the magnesium silicide raw powder can be completely converted into the magnesium silicide two-dimensional nanosheet. Further, the power of the ultrasonic crusher is 400-430W/cm2And the ultrasonic crusher works intermittently for 4-7 s and stops working for 1-3 s every time, so that the quality of the prepared magnesium silicide two-dimensional nanosheet is improved, and it should be noted that in the embodiment, the power of the ultrasonic crusher is preferably 425W/cm2。
Further, after step S20, the method further includes:
and S30, adding a modifier into the magnesium silicide two-dimensional nanosheet, centrifuging and washing to obtain the modified magnesium silicide two-dimensional nanosheet.
In step S30, the purity of the modifier is more than or equal to 99%, and the molecular weight is 40000-70000 daltons, so that the magnesium silicide two-dimensional nanosheets are prevented from being hydrolyzed in a large amount during modification, and the hydrogen amount of the subsequent hydrolysis reaction is greatly reduced.
Specifically, the modifier comprises one of polyvinylpyrrolidone, polylactic acid and polyethylene glycol.
In the above steps, the dispersibility of the magnesium silicide two-dimensional nanosheets is enhanced by the modifier, the magnesium silicide two-dimensional nanosheets are prevented from being aggregated, and the modifier in the modified magnesium silicide two-dimensional nanosheets can be washed away by the detergent, so that the use of the subsequently modified magnesium silicide two-dimensional nanosheets is prevented from being influenced by the modifier.
Specifically, in this embodiment, ethanol and acetone are selected as the detergent, and after washing with centrifugal water for 3 to 6 times, the excess polyvinylpyrrolidone in the modified magnesium silicide two-dimensional nanosheet is washed away. Of course, in other embodiments, the detergent may be selected as desired, and the invention is not limited thereto. When the modified magnesium silicide two-dimensional nanosheet prepared by the method is not used in time, the modified magnesium silicide two-dimensional nanosheet needs to be stored, and the specific storage mode is to disperse the modified magnesium silicide two-dimensional nanosheet in absolute ethyl alcohol for storage.
The invention provides a preparation method of a magnesium silicide two-dimensional nanosheet, which comprises the steps of adding raw magnesium silicide powder into an alcohol solvent, stirring and mixing to obtain a mixture A, and carrying out ultrasonic stripping on the mixture A to obtain the magnesium silicide two-dimensional nanosheet. The prepared magnesium silicide two-dimensional nano-sheet has higher activity, can carry out long-acting hydrogen slow release under physiological conditions, can be used as a main raw material of a hydrogen release dressing, and can be used for repairing and regenerating large-area burn wounds.
It should be noted that, with reference to fig. 3, an FT-IR spectrum of a magnesium silicide two-dimensional nanosheet, fig. 4, an XRD spectrum of the magnesium silicide two-dimensional nanosheet, fig. 5, a Transmission Electron Microscope (TEM) characterization spectrum of the magnesium silicide two-dimensional nanosheet, fig. 6, an Atomic Force Microscope (AFM) characterization spectrum of the magnesium silicide two-dimensional nanosheet, and fig. 7, a statistical analysis chart corresponding to the Atomic Force Microscope (AFM) characterization spectrum of the magnesium silicide two-dimensional nanosheet are shown, and by observing fig. 3 to fig. 7, the preparation process of the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet provided by the present invention does not require complicated equipment and procedures, does not require a chemical etching agent, and has high hydrogen production efficiency.
In addition, the embodiment also provides an application of the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet in preparing burn dressings; or the like, or, alternatively,
the application in preparing the material for promoting the expression of the vascular endothelial cell growth factor; or the like, or, alternatively,
the application in preparing the material for promoting the angiogenesis of deep burn wound surfaces.
Specifically, because the magnesium silicide two-dimensional nanosheet has high reactivity, the magnesium silicide two-dimensional nanosheet can be hydrolyzed in a solution environment with any pH value to generate basic magnesium silicate and hydrogen, and the reaction formula is as follows:
magnesium silicide +5H2O → magnesium silicide O3(OH)2+4H2↑
Compared with MgH, the magnesium silicide two-dimensional nanosheet hydrolysis hydrogen production material2、NaBH4The raw magnesium silicide powder is low in price and beneficial to reducing the material cost, and Mg and Si are light elements, so that the magnesium silicide II is preparedThe dimension nano-sheet has the advantages of low density, high cost performance and high unit hydrogen production rate, compared with the existing magnesium silicide raw powder, the magnesium silicide two-dimensional nano-sheet prepared by the preparation method has stronger activity, can be hydrolyzed at high concentration under acidic, neutral and alkaline conditions to produce hydrogen, and has a high-biocompatibility silicate as a product, simple preparation method and good biocompatibility. When the magnesium silicide two-dimensional nanosheet prepared by the method is used for repairing deep burn wounds, the performance is excellent, the hydrogen is released at the focus position in a long-acting and high-concentration manner, the proliferation and migration of cells at the wound position are promoted, the Vascular Endothelial Growth Factor (VEGF) expression at the wound position is up-regulated, and the generation of new blood vessels is promoted, so that the remodeling of the skin structure and the function of the wound is promoted.
Referring to fig. 2 and fig. 35, in this embodiment, the preparation method of the hydrogen-releasing hydrogel wound dressing includes the following steps:
s100, preparing a hydrogel substrate;
the step S100 includes:
s1001, providing a chitosan solution with the mass concentration of 0.5-2%, a sodium glycerophosphate solution with the mass concentration of 7-12% and a sodium hyaluronate solution with the mass concentration of 0.2-1%;
specifically, a chitosan solution with a mass concentration of 0.5-2%, a sodium glycerophosphate solution with a mass concentration of 7-12%, and a sodium hyaluronate solution with a mass concentration of 0.2-1% can be purchased directly or prepared by themselves. Specifically, in this embodiment, the preparation method of the chitosan solution with a mass concentration of 0.5-2% is to measure a proper amount of chitosan dry powder (deacetylation degree 95%) and dissolve the chitosan dry powder in 0.1M hydrochloric acid to prepare a chitosan hydrochloric acid solution with a concentration of 0.5-2%, stir until the solution is clear and transparent, and store the solution at 4 ℃ for later use. The preparation method of the sodium glycerophosphate solution with the mass concentration of 7-12% comprises the steps of weighing a proper amount of sodium hyaluronate (such as low molecular sodium hyaluronate HA-TLM 20-40) dry powder, dissolving the sodium hyaluronate dry powder in distilled water to prepare a 0.2-1% sodium hyaluronate solution, and storing the solution at 4 ℃ for later use. The preparation method of the sodium hyaluronate solution with the mass concentration of 0.2-1% comprises the steps of weighing a proper amount of sodium glycerophosphate dry powder, dissolving the sodium glycerophosphate dry powder in distilled water to prepare a 7-12% sodium glycerophosphate solution, and storing the solution at 4 ℃ for later use.
Specifically, in this embodiment, the concentration of the sodium glycerophosphate solution is 10%, and the sodium glycerophosphate solution functions as a cross-linking agent, and can generate chemical bonds between linear molecules, so that the linear molecules are connected to each other to form a network structure, thereby improving the strength and elasticity of the polymer material.
S1002, mixing the chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution according to the weight ratio of 10-60: 30: and (3) uniformly mixing the components in a volume ratio of 10-60 to prepare the hydrogel substrate.
It should be noted that the chitosan may be crab chitosan, the degree of deacetylation is 95%, and preferably, the mass concentration of the chitosan solution is 2%, so that the chitosan solution is convenient to store, and the problem that the deterioration of the chitosan solution affects subsequent use is avoided. The sodium hyaluronate can be cosmetic grade and has a molecular weight of 200-400 kDa, and preferably, the mass concentration of the sodium hyaluronate solution is 1%, so that the sodium hyaluronate solution is convenient to store and prevented from deteriorating.
In other embodiments, a 2% by mass chitosan solution, a 10% by mass sodium glycerophosphate solution and a 1% by mass sodium hyaluronate solution are mixed at room temperature and 30-40 ℃ for 5-20min to form a hydrogel substrate, wherein the volume ratio of the chitosan solution to the sodium glycerophosphate solution is 6:3:1, 5:3:2, 4:3:3, 3:3:4, 2:3:5 or 1:3: 6.
Specifically, the gel forming time is preferably 8 minutes. The optimal gel forming temperature is 37 ℃, so that the hydrogel substrate prepared at the time and temperature has the best quality.
S200, adding the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet into the hydrogel substrate, uniformly mixing, and preparing the hydrogen release hydrogel wound dressing at 30-40 ℃.
Further, mixing the prepared chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution in a volume ratio of 5:3:2, dispersing the magnesium silicide two-dimensional nanosheets into the mixed solution after uniformly mixing, and then gelatinizing in grinding tools of different shapes according to requirements at 37 ℃.
Specifically, 1-500 mL of hydrogel substrate is correspondingly added to each 1 mug of the magnesium silicide two-dimensional nanosheet, so that the magnesium silicide two-dimensional nanosheet and the hydrogel substrate are fully and uniformly mixed, and the hydrolysis efficiency of the magnesium silicide two-dimensional nanosheet is improved.
The embodiment also provides an application of the hydrogen-releasing hydrogel wound dressing prepared by the preparation method of the hydrogen-releasing hydrogel wound dressing in preparing a medical device for repairing deep burn wounds; or the like, or, alternatively,
the application in preparing medical device for promoting the vascular endothelial growth factor expression in burn wound; or the like, or, alternatively,
the application in preparing medical devices for promoting angiogenesis of deep burn wounds and/or constructing immune microenvironment for promoting tissue repair.
The prepared hydrogen release hydrogel wound dressing is dispersed with the magnesium silicide two-dimensional nanosheets, the magnesium silicide two-dimensional nanosheets have the advantages of low density, high cost performance and high unit hydrogen production rate, the activity of the magnesium silicide two-dimensional nanosheets is stronger, hydrogen can be produced through high-concentration hydrolysis under acidic, neutral and alkaline conditions, the product is a silicate with high biocompatibility, the preparation method is simple, and the biocompatibility is good. By mixing the magnesium silicide two-dimensional nano-sheet with the hydrogel substrate, the reaction of hydrolysis and hydrogen release of the magnesium silicide two-dimensional nano-sheet is mild, the process is simple, and the byproduct is alkalescent magnesium silicide O3(HO)2The biological safety is good, and the reaction process has no violent exothermic behavior. The preparation process and the used equipment of the hydrogen release gel dressing are simple and easy to implement, and no safety risk exists in the production process; the prepared hydrogen-releasing hydrogel dressing has excellent performance in application of repairing deep burn wounds, promotes the proliferation and migration of cells at the wound site by releasing hydrogen at the focus site in a long-acting and high-concentration manner, and simultaneously, up-regulates the Vascular Endothelial Growth Factor (VEGF) expression at the wound site to promote the generation of new blood vessels, thereby promoting the generation of new blood vesselsRemodeling the structure and function of the wound skin.
In addition, the substrate of the hydrogen release hydrogel dressing is chitosan and hyaluronic acid with excellent biocompatibility, the hydrogel substrate not only provides a humid environment for hydrolysis of the magnesium silicide two-dimensional nanosheets, but also provides a good extracellular matrix-like microenvironment for the burn wound, protects the wound from secondary damage caused by exogenous foreign matters, and is beneficial to remodeling of the skin structure and functions of the wound.
It is noted that the humid environment is an environment containing water, such as an aqueous solution in vitro, or a physiological environment in vivo. The aqueous environment is an environment in which water is used as a solvent or a liquid containing water is used as a solvent, and the environment is not limited to an in vivo physiological environment such as an aqueous solution, an aqueous solution containing a buffer salt, and a body fluid, blood, or tissue fluid.
The technical solutions of the present invention are further described in detail below with reference to specific examples and drawings, it should be understood that the following examples are merely illustrative of the present invention and are not intended to limit the present invention.
An embodiment of the preparation method of the magnesium silicide two-dimensional nanosheet provided by the invention is given as follows:
(1) adding magnesium silicide raw powder into at least one of acetone, ethylene glycol, ethanol and glycerol, stirring and mixing to obtain a mixture A, wherein the purity of the magnesium silicide raw powder is more than or equal to 99%, the particle size of the magnesium silicide raw powder is 5-150 mu m, the particle size of a magnesium silicide nano sheet is about 700nm, the thickness of the magnesium silicide nano sheet is about 0.8nm, the purity of an alcohol solvent is more than or equal to 99%, and 1 mg-5 g of the magnesium silicide raw powder is correspondingly added into each 1mL of the alcohol solvent;
(2) ultrasonically stripping the mixture A through an ultrasonic crusher to prepare a magnesium silicide two-dimensional nanosheet, wherein the power of the ultrasonic crusher is 100-2Preferably 400-430W/cm2And the ultrasonic crusher works intermittently, the ultrasonic crusher stops working for 1-3 s every 4-7 s, and the ultrasonic crushing time is 5-100 min.
(3) And adding one of polyvinylpyrrolidone, polylactic acid and polyethylene glycol into the magnesium silicide two-dimensional nanosheet, centrifuging and washing to obtain the modified magnesium silicide two-dimensional nanosheet, wherein the purity of the modifier is more than or equal to 99%, the molecular weight is 40000-70000 daltons, the washing agent is at least one of ethanol or acetone, and washing for 3-6 times by centrifuging and water washing.
An embodiment of the preparation method of the hydrogen-releasing hydrogel wound dressing provided by the invention is given as follows:
(1) providing a chitosan solution with the mass concentration of 0.5-2%, a sodium glycerophosphate solution with the mass concentration of 7-12% and a sodium hyaluronate solution with the mass concentration of 0.2-1%, and mixing the chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution according to the ratio of 10-60: 30: and (2) uniformly mixing the chitosan solution and the water gel substrate according to the volume ratio of 10-60 to prepare the hydrogel substrate, wherein the chitosan can be crab chitosan, the deacetylation degree is 95%, and the optimal storage mass concentration of the chitosan solution is 2%. The sodium hyaluronate can be in cosmetic grade, the molecular weight is 200-400 kDa, the optimal storage mass concentration of the sodium hyaluronate solution is 1%, the optimal gelling time is 8 minutes, and the optimal gelling temperature is 37 ℃.
(2) Adding the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet into the hydrogel substrate, uniformly mixing, and preparing the hydrogen release hydrogel wound dressing at 30-40 ℃, wherein 1-500 mL of hydrogel substrate is correspondingly added into each 1 mu g of the magnesium silicide two-dimensional nanosheet.
Example 1
(1) Adding magnesium silicide raw powder into ethylene glycol, stirring and mixing to obtain a mixture A, wherein the purity of the magnesium silicide raw powder is more than or equal to 99%, the particle size of the magnesium silicide raw powder is 5 mu m, the particle size of a magnesium silicide nano sheet is about 700nm, the thickness of the magnesium silicide nano sheet is about 0.8nm, the purity of an alcohol solvent is more than or equal to 99%, and 1mg of the magnesium silicide raw powder is correspondingly added into every 1mL of the alcohol solvent;
(2) ultrasonically stripping the mixture A through an ultrasonic crusher to prepare a magnesium silicide two-dimensional nanosheet, wherein the power of the ultrasonic crusher is 100W/cm2And the ultrasonic crusher works intermittently, the ultrasonic crusher stops working for 1s every 4s, and the ultrasonic crushing time is 5 min.
(3) And adding polyvinylpyrrolidone into the magnesium silicide two-dimensional nanosheet, centrifuging and washing to obtain the modified magnesium silicide two-dimensional nanosheet, wherein the purity of the modifier is more than or equal to 99%, the molecular weight is 40000 daltons, the detergent is ethanol, and the washing is carried out for 3 times by centrifuging and washing.
Example 2
(1) Adding magnesium silicide raw powder into ethanol, stirring and mixing to obtain a mixture A, wherein the purity of the magnesium silicide raw powder is more than or equal to 99%, the particle size of the magnesium silicide raw powder is 150 mu m, the particle size of a magnesium silicide nano sheet is about 700nm, the thickness of the magnesium silicide nano sheet is about 0.8nm, the purity of an alcohol solvent is more than or equal to 99%, and 5mg of the magnesium silicide raw powder is correspondingly added into each 1mL of the alcohol solvent;
(2) ultrasonically stripping the mixture A through an ultrasonic crusher to prepare a magnesium silicide two-dimensional nanosheet, wherein the power of the ultrasonic crusher is 1000W/cm2And the ultrasonic crusher works intermittently, the ultrasonic crushing time is 100min and the ultrasonic crushing time is 3s per 7s of work.
(3) Adding polylactic acid into the magnesium silicide two-dimensional nanosheet, centrifuging and washing to obtain the modified magnesium silicide two-dimensional nanosheet, wherein the purity of the modifier is more than or equal to 99%, the molecular weight is 40000-70000 daltons, the detergent is acetone, and the washing is carried out for 6 times by centrifugal water.
Example 3
(1) Adding magnesium silicide raw powder into glycerol, stirring and mixing to obtain a mixture A, wherein the purity of the magnesium silicide raw powder is more than or equal to 99%, the particle size of the magnesium silicide raw powder is 77.5 mu m, the particle size of a magnesium silicide nano sheet is about 700nm, the thickness of the magnesium silicide nano sheet is about 0.8nm, the purity of an alcohol solvent is more than or equal to 99%, and 3mg of the magnesium silicide raw powder is correspondingly added into every 1mL of the alcohol solvent;
(2) ultrasonically stripping the mixture A through an ultrasonic crusher to prepare a magnesium silicide two-dimensional nanosheet, wherein the power of the ultrasonic crusher is 550W/cm2And the ultrasonic crusher works intermittently, and the stop time is 2 every 5.5sAnd s, the time of ultrasonication is 55 min.
(3) And adding one of polyvinylpyrrolidone, polylactic acid and polyethylene glycol into the magnesium silicide two-dimensional nanosheet, centrifuging and washing to obtain the modified magnesium silicide two-dimensional nanosheet, wherein the purity of the modifier is more than or equal to 99%, the molecular weight is 55000 daltons, and the washing agent is ethanol and acetone and is washed for 4 times by centrifuging and water.
Example 4
(1) Adding magnesium silicide raw powder into acetone, stirring and mixing to obtain a mixture A, wherein the purity of the magnesium silicide raw powder is more than or equal to 99%, the particle size of powder of the magnesium silicide raw powder is 60 mu m, the particle size of a magnesium silicide nano sheet is about 700nm, the thickness of the magnesium silicide nano sheet is about 0.8nm, the purity of an alcohol solvent is more than or equal to 99%, and 2mg of the magnesium silicide raw powder is correspondingly added into every 1mL of the alcohol solvent;
(2) ultrasonically stripping the mixture A through an ultrasonic crusher to prepare a magnesium silicide two-dimensional nanosheet, wherein the power of the ultrasonic crusher is 420W/cm2And the ultrasonic crusher works intermittently, the stop time is 1.5s every 6s, and the ultrasonic crushing time is 65 min.
(3) And adding one of polyvinylpyrrolidone, polylactic acid and polyethylene glycol into the magnesium silicide two-dimensional nanosheet, centrifuging and washing to obtain the modified magnesium silicide two-dimensional nanosheet, wherein the purity of the modifier is more than or equal to 99%, the molecular weight is 45000 daltons, the detergent is ethanol, and the washing is carried out for 5 times by centrifuging and water washing.
Example 5
The power of the ultrasonicator described in example 1 was set to 100W/cm2Instead, 400W/cm is added2Otherwise, the procedure was the same as in example 1.
Example 6
The power of the ultrasonicator described in example 1 was set to 100W/cm2Instead, 430W/cm is added2Otherwise, the procedure was the same as in example 1.
Example 7
(1) Providing a chitosan solution with the mass concentration of 0.5%, a sodium glycerophosphate solution with the mass concentration of 7% and a sodium hyaluronate solution with the mass concentration of 0.2%, and mixing the chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution according to the ratio of 1:3: 1 to prepare the hydrogel substrate, wherein the chitosan can be crab chitosan, the deacetylation degree is 95%, and the optimal storage mass concentration of the chitosan solution is 2%. The sodium hyaluronate can be in cosmetic grade, the molecular weight is 200-400 kDa, the optimal storage mass concentration of the sodium hyaluronate solution is 1%, the optimal gelling time is 8 minutes, and the optimal gelling temperature is 37 ℃.
(2) Adding the magnesium silicide prepared by the preparation method of the magnesium silicide two-dimensional nanosheet into the hydrogel substrate in a two-dimensional mode, uniformly mixing, and preparing the hydrogen release hydrogel wound dressing at 30 ℃, wherein 1mL of hydrogel substrate is correspondingly added into each 1 mu g of the magnesium silicide two-dimensional nanosheet.
Example 8
(1) Providing a chitosan solution with the mass concentration of 2%, a sodium glycerophosphate solution with the mass concentration of 12% and a sodium hyaluronate solution with the mass concentration of 1%, and mixing the chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution according to the ratio of 6:3:1 to prepare the hydrogel substrate, wherein the chitosan can be crab chitosan, the deacetylation degree is 95%, and the optimal storage mass concentration of the chitosan solution is 2%. The sodium hyaluronate can be in cosmetic grade, the molecular weight is 200-400 kDa, the optimal storage mass concentration of the sodium hyaluronate solution is 1%, the optimal gelling time is 8 minutes, and the optimal gelling temperature is 37 ℃.
(2) Adding the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet into the hydrogel substrate, uniformly mixing, and preparing the hydrogen release hydrogel wound dressing at 40 ℃, wherein 500mL of hydrogel substrate is correspondingly added into every 1 mu g of the magnesium silicide two-dimensional nanosheet.
Example 9
(1) Providing a chitosan solution with the mass concentration of 2.25%, a sodium glycerophosphate solution with the mass concentration of 9.5% and a sodium hyaluronate solution with the mass concentration of 0.6%, and mixing the chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution according to the ratio of 6:3: 6, and preparing the hydrogel substrate, wherein the chitosan can be crab chitosan, the deacetylation degree is 95%, and the optimal storage mass concentration of the chitosan solution is 2%. The sodium hyaluronate can be in cosmetic grade, the molecular weight is 200-400 kDa, the optimal storage mass concentration of the sodium hyaluronate solution is 1%, the optimal gelling time is 8 minutes, and the optimal gelling temperature is 37 ℃.
(2) Adding the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet into the hydrogel substrate, uniformly mixing, and preparing the hydrogen release hydrogel wound dressing at 35 ℃, wherein 250.5mL of hydrogel substrate is correspondingly added into each 1 mug of the magnesium silicide two-dimensional nanosheet.
Example 10
(1) Providing a chitosan solution with the mass concentration of 1.1%, a sodium glycerophosphate solution with the mass concentration of 8% and a sodium hyaluronate solution with the mass concentration of 0.8%, and mixing the chitosan solution, the sodium glycerophosphate solution and the sodium hyaluronate solution according to the ratio of 1:3:6, and preparing the hydrogel substrate, wherein the chitosan can be crab chitosan, the deacetylation degree is 95%, and the optimal storage mass concentration of the chitosan solution is 2%. The sodium hyaluronate can be in cosmetic grade, the molecular weight is 200-400 kDa, the optimal storage mass concentration of the sodium hyaluronate solution is 1%, the optimal gelling time is 8 minutes, and the optimal gelling temperature is 37 ℃.
(2) Adding the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet into the hydrogel substrate, uniformly mixing, and preparing the hydrogen release hydrogel wound dressing at 50 ℃, wherein 300mL of hydrogel substrate is correspondingly added into every 1 mu g of the magnesium silicide two-dimensional nanosheet.
Application example 1
Under normal temperature and normal pressure, the magnesium silicide two-dimensional nanosheets are uniformly distributed in PBS with different pH values, and hydrogen release behaviors of the magnesium silicide nanosheets in liquid environments with different pH values are observed by using a hydrogen electrode.
The magnesium silicide nanosheet is prepared through an ultrasonic stripping method, and then the surface of the magnesium silicide nanosheet is modified through polyvinylpyrrolidone to improve the dispersibility of the magnesium silicide nanosheet. Then, magnesium silicide nanosheets with the concentration of 200 mug/mL are dispersed in PBS solution with pH values of 5.0, 6.8, 7.4 and 8.0, and the hydrolytic hydrogen release behavior of the magnesium silicide nanosheets in liquid environments with different pH values is detected by using a hydrogen electrode.
The experimental results show (see fig. 8 to 9, and p <0.01, > p <0.001, > p <0.0001 in each figure), that magnesium silicide nanoplates can release hydrogen gas at high concentrations for a long period of time for nearly one week, whether acidic, alkaline or neutral, and maintain a high concentration of hydrogen release behavior over the first three days.
Application example 2
Under normal temperature and pressure, the magnesium silicide two-dimensional nano-sheets are uniformly distributed in the chitosan/hyaluronic acid gel, and PBS solution with volume fraction of 5% is added into the gel to simulate the pH value of the microenvironment in the wound repair process. The hydrogen release behavior of the hydrogen-releasing hydrogel dressing in liquid environments with different pH values was observed using a hydrogen electrode.
The experimental results showed that (see fig. 8-9, and p <0.01, # p <0.001, # p <0.0001) the hydrogel substrate did not affect the hydrogen evolution behavior of the magnesium silicide nanoplates. The hydrogen-releasing hydrogel dressing can release hydrogen gas at a high concentration for a long time and maintain a high-efficient hydrogen-releasing behavior for the first three days, whether in acidic, alkaline or neutral state.
Application example 3
The magnesium silicide two-dimensional nanosheets prepared in example 1 facilitate studies of cell proliferation and migration ability in vitro. And (3) selecting classical keratinocyte, fibroblast and vascular endothelial cell lines, and evaluating the influence of the magnesium silicide two-dimensional nanosheet on cell proliferation and migration in vitro. By using Na2SiO3NaOH and MgCl2Powder is prepared by mixing the following components in a molar ratio of 1: 2: 2 preparing a reaction product (MSO) buffer solution to remove Mg in the reaction product2+And SiO3 2-The influence of (c). By arranging a magnesium silicide two-dimensional nano-sheet containing 200 mug/mL and a pair of the two-dimensional nano-sheetsAnd (3) detecting the influence of hydrogen released by the magnesium silicide two-dimensional nanosheets on cell proliferation and migration capacity by a culture solution of a reaction product with a corresponding concentration through CCK-8 and a cell scratch experiment.
The experimental results showed (refer to fig. 10 to 18, and p in each figure)<0.01,***p<0.001,****p<0.0001), due to Mg2+And SiO3 2-The effect of (a), the proliferation and migration capacity of the cells in the MSO group are promoted to some extent. But compared with other experimental groups, the proliferation and migration capacity of cells in the magnesium silicide two-dimensional nano-chip group are obviously improved. This demonstrates the ability of the hydrogen generated by hydrolysis of the magnesium silicide two-dimensional nanoplates to significantly promote proliferation and migration of cells.
Application example 4
Study of the angiogenesis promoting ability of hydrogen gas released from the hydrogen-releasing hydrogel dressing in example 7 in vitro. The effect of hydrogen on the angiogenic capacity of vascular endothelial cells (HMEC-1) was explored in vitro by means of a hydrogen incubator. The experimental results showed (see fig. 19 to 20, and p in each figure)<0.01,***p<0.001,****p<0.0001), with CO2The vascular endothelial cells in the hydrogen incubator formed more vessels than the experimental group cultured in the incubator. This suggests that hydrogen can significantly promote vascularization of vascular endothelial cells.
Application example 5
Evaluation of the ability of the hydrogen-releasing hydrogel dressing in example 7 to promote the repair of a second-degree burn wound.
And evaluating the capability of the hydrogen-releasing hydrogel dressing on repairing the deep II-degree burn wound surface by establishing a mouse deep II-degree burn model. Reaction products (Mg) were excluded by dispersing MSO buffer solutions corresponding to the concentrations of magnesium silicide nanoplates in hydrogel base stock solution and after gelling for the MSO treatment group2+And SiO3 2-) Influence on burn wound repair.
The experimental results showed (referring to fig. 21 to 26, scale in fig. 21 is 1cm, and p <0.01, # p <0.001, # p <0.0001 in each figure) that there were a large number of viable keratinocytes and fibroblasts in the wound surface of the treatment group using the hydrogen-releasing hydrogel dressing, indicating that keratinocytes and fibroblasts successfully migrated from the surrounding normal tissue to the wound site, compared to the other experimental groups. In addition, the hydrogen-releasing hydrogel dressing also inhibits the infiltration of inflammatory cells into the dermis, probably because the released hydrogen molecules have an anti-inflammatory effect, which may be an important reason for reducing collagen distribution and enhancing fibroblast proliferation. In the later period, the thickness of the dermis layer and the whole skin layer of the hydrogen-releasing hydrogel dressing treatment group is remarkably improved compared with other groups.
Application example 6
Evaluation of the ability of the hydrogen-releasing hydrogel dressing in example 7 to promote regeneration of blood vessels within the wound surface of a degree II burn.
Immunohistochemical staining and Western Blot are adopted to detect the influence of the hydrogen release hydrogel dressing on the expression of Vascular Endothelial Growth Factor (VEGF), platelet-endothelial cell adhesion molecule (CD31), alpha-smooth muscle actin (alpha-SMA) and the like in the deep II-degree burn wound tissues.
The morphology and size of wound new blood vessels in the middle (day 7) and later (day 14) stages of treatment are identified by immunohistochemical staining of platelet endothelial cell adhesion molecule (CD31) and alpha-smooth muscle actin (alpha-SMA). From the 7-day immunofluorescent-stained sections (see fig. 27 to 34, where p <0.01, p <0.001, p <0.0001), it can be seen that the hydrogen-releasing hydrogel dressing group induced significantly more expression of CD31 and α -SMA than the other three experimental groups, meaning that there was more neovascularization during the initial stages of wound healing. From the 14-day fluorescent stained sections (see fig. 27 to 34), it can be seen that the diameter of the blood vessels regenerated by the hydrogen-releasing hydrogel dressing group is larger than that of the other experimental groups, and is closer to the normal skin, because the hydrogen gas released by the hydrogen-releasing hydrogel dressing can promote the generation of the blood vessels.
The influence of the hydrogen-releasing hydrogel dressing on the expression of Vascular Endothelial Growth Factor (VEGF) and platelet-endothelial cell adhesion molecule (CD31) in the deep II-degree burn wound tissues is detected by Western blotting. The results show (see fig. 27 to 34) that the hydrogen-releasing hydrogel dressing significantly enhanced the expression of VEGF in the middle (day 7) and later (day 14) stages of wound treatment, promoted angiogenesis, and promoted the expression of CD 31. The hydrogen released by the hydrogen-releasing hydrogel dressing is helpful for the expression of VEGF and the activation of vascular endothelial cells, the repair of burn wound surfaces is promoted, and the remodeling of the skin structure and function of the burn wound surfaces is facilitated.
The above is only a preferred embodiment of the present invention, and it is not intended to limit the scope of the invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall be included in the scope of the present invention.
Claims (10)
1. A preparation method of a magnesium silicide two-dimensional nanosheet is characterized by comprising the following steps:
s10, adding the magnesium silicide raw powder into an alcohol solvent, stirring and mixing to obtain a mixture A;
s20, carrying out ultrasonic stripping on the mixture A to obtain the magnesium silicide two-dimensional nanosheet.
2. A method for preparing magnesium silicide two-dimensional nanoplatelets as in claim 1 wherein, in step S10,
the alcohol solvent comprises at least one of acetone, glycol, ethanol and glycerol.
3. The preparation method of the magnesium silicide two-dimensional nanosheets as defined in claim 1, wherein 1 mg-5 g of the magnesium silicide raw powder is added to 1mL of the alcohol solvent.
4. The method for producing magnesium silicide two-dimensional nanoplatelets of claim 1 wherein, in step S20, ultrasonic exfoliation is performed by an ultrasonicator.
5. The preparation method of the magnesium silicide two-dimensional nanosheets as defined in claim 4, wherein the power of the ultrasonication apparatus is 100-1000W/cm2Ultrasound (ultrasound)The crushing time is 5-100 min.
6. A preparation method of the hydrogen release hydrogel wound dressing is characterized by comprising the following steps:
s100, preparing a hydrogel substrate;
s200, adding the magnesium silicide two-dimensional nanosheet prepared by the preparation method of the magnesium silicide two-dimensional nanosheet as claimed in any one of claims 1 to 5 into the hydrogel substrate, and uniformly mixing to prepare the hydrogel wound dressing.
7. The preparation method of the hydrogen-releasing hydrogel wound dressing as claimed in claim 6, wherein 1-500 mL of hydrogel substrate is added for every 1 μ g of the magnesium silicide two-dimensional nanosheet.
8. Use of the magnesium silicide two-dimensional nanoplates prepared by the method of preparing magnesium silicide two-dimensional nanoplates as described in any one of claims 1 to 5 in the preparation of burn dressings.
9. Use of the magnesium silicide two-dimensional nanoplates prepared by the method of preparing magnesium silicide two-dimensional nanoplates of any of claims 1 to 5 in the preparation of a material that promotes the expression of vascular endothelial cell growth factor; or the like, or, alternatively,
the application in preparing the material for promoting the angiogenesis of deep burn wound surfaces.
10. Use of a hydrogel wound dressing prepared by the method of preparing a hydrogel wound dressing according to claim 6 or 7 in the preparation of a medical device for repairing a deep burn wound; or the like, or, alternatively,
the application in preparing medical device for promoting the vascular endothelial growth factor expression in burn wound; or the like, or, alternatively,
the application in preparing medical devices for promoting angiogenesis of deep burn wounds and/or constructing immune microenvironment for promoting tissue repair.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006321792A (en) * | 2005-04-18 | 2006-11-30 | Showa Denko Kk | Water-bearing gel and its production method |
| CN104261411A (en) * | 2014-09-22 | 2015-01-07 | 江苏大学 | Preparation method of graphene-like magnesium silicide nanosheets |
| CN106913582A (en) * | 2015-12-28 | 2017-07-04 | 中国科学院上海硅酸盐研究所 | A kind of magnesium silicide nano material and its preparation method and application |
| CN107585738A (en) * | 2017-07-21 | 2018-01-16 | 华南理工大学 | A kind of Mg Mg2Si composite hydrolysis hydrogen manufacturing materials and preparation method thereof and the method for hydrolytic hydrogen production |
| CN112263777A (en) * | 2020-08-04 | 2021-01-26 | 北京工业大学 | Hydrogen molecule slow-release composite dressing and preparation method thereof |
| CN112972773A (en) * | 2021-02-04 | 2021-06-18 | 深圳先进技术研究院 | Bioactive bone composite material and preparation method and application thereof |
-
2022
- 2022-01-21 CN CN202210076555.9A patent/CN114380292B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006321792A (en) * | 2005-04-18 | 2006-11-30 | Showa Denko Kk | Water-bearing gel and its production method |
| CN104261411A (en) * | 2014-09-22 | 2015-01-07 | 江苏大学 | Preparation method of graphene-like magnesium silicide nanosheets |
| CN106913582A (en) * | 2015-12-28 | 2017-07-04 | 中国科学院上海硅酸盐研究所 | A kind of magnesium silicide nano material and its preparation method and application |
| CN107585738A (en) * | 2017-07-21 | 2018-01-16 | 华南理工大学 | A kind of Mg Mg2Si composite hydrolysis hydrogen manufacturing materials and preparation method thereof and the method for hydrolytic hydrogen production |
| CN112263777A (en) * | 2020-08-04 | 2021-01-26 | 北京工业大学 | Hydrogen molecule slow-release composite dressing and preparation method thereof |
| CN112972773A (en) * | 2021-02-04 | 2021-06-18 | 深圳先进技术研究院 | Bioactive bone composite material and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| L. MARQUEZ-GARCIA ET AL.: "Preparation of Nanoparticles of Thermoelectric Materials by Ultrasonic Milling", 《JOURNAL OF ELECTRONIC MATERIALS》 * |
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