CN114350487A - Operating instrument for unicellular sampling and quick replacement of spray needle - Google Patents
Operating instrument for unicellular sampling and quick replacement of spray needle Download PDFInfo
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- CN114350487A CN114350487A CN202011092966.4A CN202011092966A CN114350487A CN 114350487 A CN114350487 A CN 114350487A CN 202011092966 A CN202011092966 A CN 202011092966A CN 114350487 A CN114350487 A CN 114350487A
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- connecting rod
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- needle
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- 238000005070 sampling Methods 0.000 title claims abstract description 64
- 239000007921 spray Substances 0.000 title claims abstract description 22
- 238000006073 displacement reaction Methods 0.000 claims abstract description 16
- 230000007246 mechanism Effects 0.000 claims abstract description 13
- 230000008859 change Effects 0.000 claims description 4
- 239000000523 sample Substances 0.000 abstract description 16
- 238000001514 detection method Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- 230000008569 process Effects 0.000 description 4
- 238000001819 mass spectrum Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The invention provides an operation instrument for single cell sampling and quick needle replacement. The sampling device comprises a workbench, a supporting structure and a single cell sampling device fixed on the supporting structure, wherein the single cell sampling device and the supporting structure have a 45-degree angle inclined downwards, the single cell sampling device comprises a movable slide rail and a movable sampling mechanism connected to the movable slide rail in a sliding manner, the movable sampling mechanism comprises a movable slide block and a hollow connecting rod fixedly connected above the movable slide block, the front end of the hollow connecting rod is detachably connected with a sampling spray needle through a needle clamping locking piece, two ends of the movable slide rail are provided with limiting blocks used for limiting the displacement of the movable sampling mechanism, the limiting block positioned below is a sampling state positioning block, and the limiting block positioned above is a sampling state positioning block. The single cell sampling needle point is convenient to replace, the sampling probe can be quickly replaced after one-time sampling, and meanwhile, the movable sliding block is locked and fixed at any position, so that the single cell high-throughput detection is facilitated.
Description
Technical Field
The invention relates to the technical field of single cell sampling, in particular to an operation instrument for quickly replacing a single cell sampling and a spray needle.
Background
The single cell analysis technology is a technology for detecting trace metabolites in single cells by a high-sensitivity and high-specificity method. The existing single cell sampling method applied to fresh animal and plant tissues mainly extracts cell solution by inserting a very fine capillary probe into a single cell. For example, CN105713829B discloses a single cell sampling device and method based on porous metal enrichment probe, which comprises a base, a support rod, a clamping device, a differential head and a fixed seat, and the sampling method is simple, rapid and cheap. The publication No. CN105575752B discloses a dual-function equal-inner-diameter skin flow mass spectrum spray needle and a preparation method thereof, discloses a preparation method of the mass spectrum spray needle, and also discloses a sampling method of the mass spectrum spray needle. The prior art lacks the high accuracy control to sampling needle point position, lacks the maneuverability at changing ionization spray needle in-process, only gives and receives liter electric spray needle position application mode, the change that is not convenient for receive liter spray needle many times relapse, and it is not convenient enough to change. This is not conducive to rapid, high-throughput detection of single cells.
Disclosure of Invention
In accordance with the above-mentioned technical problem, a manipulator for single cell sampling and rapid needle replacement is provided. The technical means adopted by the invention are as follows:
the utility model provides an operation appearance for unicellular sample and spray needle quick replacement, includes workstation, bearing structure and fixes the unicellular sampling device on bearing structure, unicellular sampling device has the angle of downward sloping with bearing structure, unicellular sampling device includes removal slide rail and sliding connection and advances the sampling mechanism at the removal slide rail removal, it includes the hollow connecting rod of removal slider and fixed connection in its top to remove the sampling mechanism, the front end of hollow connecting rod is connected with a kind spray needle through double-layered needle retaining member detachably, the both ends of removal slide rail are equipped with the stopper that is used for the restriction to remove the displacement of sampling mechanism, and the stopper that is located the below is the sampling state locating piece, and the stopper that is located the top is the sampling state locating piece.
Further, the supporting structure is a three-dimensional adjusting frame, and the bottom of the three-dimensional adjusting frame is connected with the bottom supporting connecting plate through a bottom supporting connecting rod.
Further, the hollow connecting rod is fixed on the hollow connecting rod bottom fixing piece through the hollow connecting rod front end locking piece and the hollow connecting rod rear end locking piece, and the hollow connecting rod bottom fixing piece is arranged on the movable sliding block.
Furthermore, one side wall of the movable sliding block is provided with a hole channel, and the sliding block stopping locking piece is screwed at the hole channel and used for fixing the movable sliding block at a preset position of the sliding rail.
According to the invention, the sampling spray needle after sampling can be conveniently and rapidly moved to the preset position through the manual or electric control three-dimensional adjusting frame, the sampling needle point is connected with the device with rapid extraction and self-locking functions, the rapid replacement of the single-cell sampling needle point can be realized, the effective ionization of the single-cell sample can be realized, and the single-cell high-throughput detection is facilitated. Meanwhile, the movable sliding rail with the sliding block moves to a specific position when the spray needle moves in the moving process, if the needle point is required to be positioned and maintained, the sliding block can be stopped to fix the sliding block at the specific position by rotating the sliding block, the sampling spray needle can be replaced at the moment, and the operation is convenient.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of the main structure of the present invention.
FIG. 2 is a front view showing the state of replacing the needle according to the present invention.
FIG. 3 is a front view of the cell sampling state of the present invention.
Fig. 4 is a side view of the present invention.
In the figure: 1. a bottom support connection plate; 2. a bottom support connecting rod; 3. an X-axis displacement platform connection; 11. x-axis displacement orbit determination; 12. a Y-axis drive motor; 13. y-axis displacement orbit determination; 14. a Y-axis movable slide rail; 15. the Y shaft is connected with the Z shaft through a supporting plate; 16. z-axis displacement orbit determination; 17. an X-axis movable slide rail; 18. a Z-axis transmission motor; 21. a single cell sampling device; 22. moving the sample injection mechanism; 23. the Z-axis moves the slide rail.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 4, the invention discloses an operation instrument for single cell sampling and rapid nozzle needle replacement, which comprises a workbench, a support structure and a single cell sampling device 21 fixed on the support structure, wherein the single cell sampling device and the support structure have a downward inclination angle which is 45 degrees in the embodiment, the single cell sampling device comprises a Z-axis movable slide rail and a movable sampling mechanism 22 connected on the Z-axis movable slide rail 23 in a sliding manner, the movable sampling mechanism comprises a movable slide block and a hollow connecting rod fixedly connected above the movable slide block, the front end of the hollow connecting rod is detachably connected with a sampling nozzle needle through a needle clamping locking piece, the single cell sampling device has two general positions, and the first position is that the slide block provided with a needle point moves to the bottom end of the slide rail, so that the single cell sampling process is realized. The second position is to move the slide block with the needle tip to the top end of the slide rail, and at the moment, the position is used for taking away the ionization spray needle and replacing the sampling spray needle, so that next single cell sampling is realized. Therefore, two ends of the Z-axis movable slide rail are provided with limiting blocks for limiting the displacement of the movable sampling mechanism, the limiting block positioned below is a sampling state positioning block, and the limiting block positioned above is a sampling state positioning block.
The supporting structure is a three-dimensional adjusting frame, and the bottom of the three-dimensional adjusting frame is connected with a bottom supporting and connecting plate 1 through a bottom supporting and connecting rod 2. Specifically, in this embodiment, the X-axis displacement fixed rail 11 is connected to the upper surface of the bottom support connection plate through the X-axis displacement platform connection member 3, the X-axis movable slide rail 17 can slide on the X-axis displacement fixed rail 11, the Y-axis displacement fixed rail 13 is connected to the upper surface of the X-axis movable slide rail 17, the Y-axis movable slide rail 14 can slide on the Y-axis displacement fixed rail 13, the Y-axis movable slide rail 14 and the Z-axis displacement fixed rail 16 are connected to the Z-axis connection support plate 15 through a 90-degree Y-axis, and the Z-axis movable slide rail can slide on the Z-axis displacement fixed rail 16.
The hollow connecting rod passes through hollow connecting rod front end retaining member and hollow connecting rod rear end retaining member to be fixed on hollow connecting rod bottom mounting, hollow connecting rod bottom mounting sets up on removing the slider.
One side wall of the movable sliding block is provided with a pore channel, and a sliding block stopping locking piece is screwed at the pore channel and is used for fixing the movable sliding block at a preset position of the sliding rail. During the moving process of the moving slide rail with the slide block, when the needle moves to a specific position, if the needle point is required to be positioned and kept uncertain, the slide block connected with the slide block stops the locking piece and rotates clockwise. At the moment, the sliding block connected with the spray needle is fixed at a specific position, the sampling spray needle can be replaced at the moment, and the operation is convenient.
The operation instrument of the invention is divided into two operation processes. One is the unicellular sample, and the removal slider that has put the sample needle point under this mode is placed in the slide rail bottom, stops through the slider and prevents the slider and drop, through manual or automatically controlled controlling for three-dimensional adjustment frame drives the sample needle point displacement, realizes the micro-manipulation sample of unicellular under microscope observation, can realize the sample to unicellular specific part, when arranging the sample location in, utilizes the micro-nano operation needle point to realize the micro-manipulation to the unicellular sample, extracts the intracellular sample through the negative pressure. The other mode is a spray needle quick replacement mode, firstly, the three-dimensional micro-operation slide rail is adjusted, at the moment, the slide rail is moved to a reference position, the sampling needle point is slightly lifted from the cell culture solution, the position of the sampling needle point is arranged at the top end of the slide rail by moving the slide block, and then the slide block is fixed at a certain position of the slide rail through a jackscrew. The slider with the needle point can not slide in the bottom because of the effect of gravity, conveniently takes away the taking of sample needle to change new needle, continue the operation of next round. The sampling needle point is connected with the device with quick extraction and self-locking functions, so that the single cell sampling needle point can be quickly replaced, and the effective ionization of the single cell sample can be realized.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (4)
1. The utility model provides an operation appearance for unicellular sample and spray needle quick replacement, a serial communication port, include workstation, bearing structure and fix the unicellular sampling device on bearing structure, unicellular sampling device has the angle of downward sloping with bearing structure, unicellular sampling device includes that moving slide rail and sliding connection move on moving slide rail and advance a kind mechanism, it includes the hollow connecting rod of removal slider and fixed connection in its top to move a kind mechanism, the front end of hollow connecting rod is connected with a kind spray needle through double-layered needle retaining member detachably, the both ends of moving slide rail are equipped with the stopper that is used for the restriction to remove a kind mechanism displacement, and the stopper that is located the below is the sampling state locating piece, and the stopper that is located the top is the sampling state locating piece.
2. The manipulator of claim 1, wherein the support structure is a three-dimensional adjusting frame, and the bottom of the three-dimensional adjusting frame is connected to the bottom support connection plate through a bottom support connection rod.
3. The manipulator according to claim 1 or 2, wherein said hollow connecting rod is fixed to a hollow connecting rod bottom end fixture by a hollow connecting rod front end fixture and a hollow connecting rod rear end fixture, said hollow connecting rod bottom end fixture being disposed on the movable slide.
4. The manipulator for single cell sampling and rapid needle change according to claim 1 or 2, wherein one of the side walls of the movable slide is provided with a hole, and a slide stop lock is screwed at the hole and used for fixing the movable slide at a preset position of the slide rail.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011092966.4A CN114350487A (en) | 2020-10-13 | 2020-10-13 | Operating instrument for unicellular sampling and quick replacement of spray needle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011092966.4A CN114350487A (en) | 2020-10-13 | 2020-10-13 | Operating instrument for unicellular sampling and quick replacement of spray needle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114350487A true CN114350487A (en) | 2022-04-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011092966.4A Pending CN114350487A (en) | 2020-10-13 | 2020-10-13 | Operating instrument for unicellular sampling and quick replacement of spray needle |
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| CN (1) | CN114350487A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020064886A1 (en) * | 2000-11-24 | 2002-05-30 | Takao Nakagawa | Apparatus and method for sampling |
| US20040257561A1 (en) * | 2000-11-24 | 2004-12-23 | Takao Nakagawa | Apparatus and method for sampling |
| CN101067259A (en) * | 2007-03-16 | 2007-11-07 | 吴宇芯 | Knitting machine cam guide block |
| CN101587029A (en) * | 2008-05-21 | 2009-11-25 | 同方威视技术股份有限公司 | Sample processing system and method for trace quantity detector |
| CN104457486A (en) * | 2014-12-16 | 2015-03-25 | 江苏天宏自动化科技有限公司 | Adjustable detection contact |
| CN104901126A (en) * | 2015-06-10 | 2015-09-09 | 苏州市吴中区胥口广博模具加工厂 | Inserting needle three-needle feeding mechanism of parallel six-needle female plug needle inserter |
| CN205120452U (en) * | 2015-10-27 | 2016-03-30 | 山东省科学院海洋仪器仪表研究所 | Organic pollutant enrichment device in sea water |
| CN205710741U (en) * | 2016-03-18 | 2016-11-23 | 中国科学院长春应用化学研究所 | A kind of unicellular sampler based on porous metals enrichment probe |
| CN107513491A (en) * | 2017-09-29 | 2017-12-26 | 河北工程大学 | Streak inoculation device |
| CN207463557U (en) * | 2017-10-27 | 2018-06-08 | 孝感华工高理电子有限公司 | A kind of dispenser for preventing syringe needle from colliding |
| CN108220122A (en) * | 2017-12-30 | 2018-06-29 | 深圳市阿瑟医疗机器人有限公司 | Method, system and the laboratory of PCR reactions are carried out using robot |
| CN109799360A (en) * | 2019-01-09 | 2019-05-24 | 东软威特曼生物科技（沈阳）有限公司 | Automatic clinical chemistry analyzer and its sampling needle system |
-
2020
- 2020-10-13 CN CN202011092966.4A patent/CN114350487A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020064886A1 (en) * | 2000-11-24 | 2002-05-30 | Takao Nakagawa | Apparatus and method for sampling |
| US20040257561A1 (en) * | 2000-11-24 | 2004-12-23 | Takao Nakagawa | Apparatus and method for sampling |
| CN101067259A (en) * | 2007-03-16 | 2007-11-07 | 吴宇芯 | Knitting machine cam guide block |
| CN101587029A (en) * | 2008-05-21 | 2009-11-25 | 同方威视技术股份有限公司 | Sample processing system and method for trace quantity detector |
| CN104457486A (en) * | 2014-12-16 | 2015-03-25 | 江苏天宏自动化科技有限公司 | Adjustable detection contact |
| CN104901126A (en) * | 2015-06-10 | 2015-09-09 | 苏州市吴中区胥口广博模具加工厂 | Inserting needle three-needle feeding mechanism of parallel six-needle female plug needle inserter |
| CN205120452U (en) * | 2015-10-27 | 2016-03-30 | 山东省科学院海洋仪器仪表研究所 | Organic pollutant enrichment device in sea water |
| CN205710741U (en) * | 2016-03-18 | 2016-11-23 | 中国科学院长春应用化学研究所 | A kind of unicellular sampler based on porous metals enrichment probe |
| CN107513491A (en) * | 2017-09-29 | 2017-12-26 | 河北工程大学 | Streak inoculation device |
| CN207463557U (en) * | 2017-10-27 | 2018-06-08 | 孝感华工高理电子有限公司 | A kind of dispenser for preventing syringe needle from colliding |
| CN108220122A (en) * | 2017-12-30 | 2018-06-29 | 深圳市阿瑟医疗机器人有限公司 | Method, system and the laboratory of PCR reactions are carried out using robot |
| CN109799360A (en) * | 2019-01-09 | 2019-05-24 | 东软威特曼生物科技（沈阳）有限公司 | Automatic clinical chemistry analyzer and its sampling needle system |
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