CN114350486A - A spray needle interface arrangement for unicellular sample - Google Patents
A spray needle interface arrangement for unicellular sample Download PDFInfo
- Publication number
- CN114350486A CN114350486A CN202011092178.5A CN202011092178A CN114350486A CN 114350486 A CN114350486 A CN 114350486A CN 202011092178 A CN202011092178 A CN 202011092178A CN 114350486 A CN114350486 A CN 114350486A
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- Prior art keywords
- connecting rod
- hollow connecting
- movable
- slide rail
- needle
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- 239000007921 spray Substances 0.000 title claims description 14
- 238000005070 sampling Methods 0.000 claims abstract description 24
- 230000007246 mechanism Effects 0.000 claims abstract description 13
- 238000006073 displacement reaction Methods 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 abstract description 14
- 238000002347 injection Methods 0.000 abstract description 7
- 239000007924 injection Substances 0.000 abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 7
- 238000000605 extraction Methods 0.000 description 3
- 238000001819 mass spectrum Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a nozzle needle interface device for single cell sampling. The movable sample injection mechanism comprises a movable slide rail and a movable sample injection mechanism which is connected to the movable slide rail in a sliding mode, wherein the movable sample injection mechanism comprises a movable sliding block and a hollow connecting rod fixedly connected above the movable sliding block, the front end of the hollow connecting rod is detachably connected with a sample injection needle through a needle clamping locking piece, and two ends of the movable slide rail are provided with limiting blocks used for limiting the displacement of the movable sample injection mechanism. The single cell sampling needle point is convenient to replace, the sampling probe can be quickly replaced after one-time sampling, and meanwhile, the movable sliding block is locked and fixed at any position, so that the single cell high-throughput detection is facilitated.
Description
Technical Field
The invention relates to the technical field of single cell sampling, in particular to a nozzle needle interface device for single cell sampling.
Background
The single cell analysis technology is a technology for detecting trace metabolites in single cells by a high-sensitivity and high-specificity method. The existing single cell sampling method applied to fresh animal and plant tissues mainly extracts cell solution by inserting a very fine capillary probe into a single cell. For example, CN105713829B discloses a single cell sampling device and method based on porous metal enrichment probe, which comprises a base, a support rod, a clamping device, a differential head and a fixed seat, and the sampling method is simple, rapid and cheap. The publication No. CN105575752B discloses a dual-function equal-inner-diameter skin flow mass spectrum spray needle and a preparation method thereof, discloses a preparation method of the mass spectrum spray needle, and also discloses a sampling method of the mass spectrum spray needle. The prior art lacks the high accuracy control to sampling needle point position, lacks the maneuverability at changing ionization spray needle in-process, only gives and receives liter electric spray needle position application mode, the change that is not convenient for receive liter spray needle many times relapse, and it is not convenient enough to change. This is not conducive to rapid, high-throughput detection of single cells.
Disclosure of Invention
In accordance with the above-mentioned technical problem, a needle interface device for single cell sampling is provided. The technical means adopted by the invention are as follows:
the utility model provides a spray needle interface arrangement for unicellular sample, advances kind the mechanism including moving slide rail and the removal of sliding connection on moving slide rail, it advances kind the mechanism including moving slider and fixed connection at the hollow connecting rod of its top to move, the front end of hollow connecting rod is connected with a kind spray needle through double-layered needle retaining piece detachably, the both ends of moving slide rail are equipped with the stopper that is used for the restriction to remove and advances kind the mechanism displacement.
Further, the hollow connecting rod is fixed on the hollow connecting rod bottom fixing piece through the hollow connecting rod front end locking piece and the hollow connecting rod rear end locking piece, and the hollow connecting rod bottom fixing piece is arranged on the movable sliding block.
Furthermore, one side wall of the movable sliding block is provided with a hole channel, and the sliding block stopping locking piece is screwed at the hole channel and used for fixing the movable sliding block at a preset position of the sliding rail.
The invention has the following advantages:
1. the sampling needle point is connected with the device of auto-lock locking through having quick extraction, can realize the quick replacement to unicellular sampling needle point, realizes the effective ionization to the unicellular sample, helps unicellular high-throughput to detect.
2. Two fixing parts with positioning properties are placed at the front end and the rear end of the sliding rail, and the sliding block can be effectively positioned at a specific position in the process of moving the front position and the rear position, so that the effect of preventing the sliding block from being separated from the sliding rail is achieved.
3. The movable sliding rail with the sliding block moves to a specific position when the needle moves, if the needle tip is required to be positioned and maintained, the sliding block can be fixed at the specific position by rotating the sliding block to stop the locking piece, the sampling needle can be replaced at the moment, and the operation is convenient.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is an exploded view of the present invention.
Fig. 3 is a side view of the present invention.
FIG. 4 is a top view of the present invention.
In the figure: 1. a sample injection needle; 2. a needle clamping locking piece; 3. a hollow connecting rod; 4. a hollow connecting rod front end locking piece; 5. a hollow connecting rod rear end locking piece; 6. a hollow connecting rod bottom end fixing piece; 7. a slide rail blocking member; 8. the slide block stops locking; 9. moving the slide block; 10. and moving the slide rail.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in figures 1-4, this embodiment discloses a needle interface arrangement for unicellular sampling, including removal slide rail 10 and sliding connection move the sampling mechanism on removing the slide rail, it includes removal slider 9 and fixed connection at its top hollow connecting rod 3 to move the sampling mechanism, conveniently at stock rear end negative pressure, extracts the cell solution of needle point front end. The front end of hollow connecting rod is connected with a kind injection spray needle 1 through 2 detachably of double-layered needle locking piece, the both ends of moving slide rail are equipped with the stopper that is used for the restriction to remove the displacement of advancing kind mechanism, the elastic component of double-layered needle locking piece for having the locking function, above-mentioned structure can effectually be taken a sample the needle point, the convenient extraction, and the mode of extraction wherein is for connecting the joint of double-layered needle locking piece and unscrew, and the sample spray needle can easily take off.
The hollow connecting rod passes through hollow connecting rod front end retaining member 4 and hollow connecting rod rear end retaining member 5 to be fixed on hollow connecting rod bottom mounting 6, hollow connecting rod bottom mounting sets up on removing the slider. In this embodiment, the hollow connecting rod front end locking piece and the hollow connecting rod rear end locking piece are the same in specification, specifically are cube structures, and the recess that holds the hollow connecting rod external diameter is seted up to the lower terminal surface of the two, and it is vertical still to have seted up the installation screw.
One side wall of the movable sliding block is provided with a hole, and the sliding block stop locking piece 8 is screwed in the hole and used for fixing the movable sliding block at a preset position of the sliding rail. The slide rail in this embodiment is "C type" metal section bar, and the slider can be high-accuracy on the slide rail, gapless removal, through outside retaining member, can be with the effectual fixed slide rail optional position. When the locking piece is loosened, the sliding block and the sliding rail can realize the movement of lower friction force, and the sliding rail blocking piece 7 is respectively arranged at the front end and the rear end of the sliding rail in order to avoid the sliding block from separating from the sliding rail.
The device can realize the rapid and gapless sliding of the sampling needle point under the one-dimensional scale, and meanwhile, the accurate stopping of the movable sliding block can be realized through locking the jackscrew at a specific position.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (3)
1. The utility model provides a spray needle interface arrangement for unicellular sample, its characterized in that, including moving slide rail and the removal sampling mechanism of sliding connection on moving slide rail, remove sampling mechanism including moving slider and the hollow connecting rod of fixed connection in its top, the front end of hollow connecting rod is connected with the appearance spray needle through double-layered needle retaining member detachably, the both ends of moving slide rail are equipped with the stopper that is used for the restriction to remove sampling mechanism's displacement.
2. The needle interface of unicellular sampling of claim 1, wherein said hollow connecting rod is fixed to a hollow connecting rod bottom end fixture by a hollow connecting rod front end lock and a hollow connecting rod rear end lock, said hollow connecting rod bottom end fixture being disposed on a movable slider.
3. The needle interface device for single cell sampling according to claim 1 or 2, wherein one of the side walls of the movable slider is provided with a hole, and a slider stop locking member is screwed at the hole and used for fixing the movable slider at a preset position of the slide rail.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011092178.5A CN114350486A (en) | 2020-10-13 | 2020-10-13 | A spray needle interface arrangement for unicellular sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011092178.5A CN114350486A (en) | 2020-10-13 | 2020-10-13 | A spray needle interface arrangement for unicellular sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114350486A true CN114350486A (en) | 2022-04-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011092178.5A Pending CN114350486A (en) | 2020-10-13 | 2020-10-13 | A spray needle interface arrangement for unicellular sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114350486A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5494039A (en) * | 1993-07-16 | 1996-02-27 | Cryomedical Sciences, Inc. | Biopsy needle insertion guide and method of use in prostate cryosurgery |
| CN202562934U (en) * | 2012-05-18 | 2012-11-28 | 济南美医林电子仪器有限公司 | Full-automatic hemorrheology sample introduction system |
| CN104111344A (en) * | 2014-05-23 | 2014-10-22 | 苏州日和科技有限公司 | Fully automatic sample-injection mechanism of flow cytometer |
| CN206573356U (en) * | 2017-03-15 | 2017-10-20 | 中山众康医疗器械有限公司 | A kind of automatic Thinprep pap test pelleter sample-adding needle construction |
| CN208500977U (en) * | 2018-06-27 | 2019-02-15 | 武汉大学 | A kind of novel cell scoring devices |
| CN109799360A (en) * | 2019-01-09 | 2019-05-24 | 东软威特曼生物科技（沈阳）有限公司 | Automatic clinical chemistry analyzer and its sampling needle system |
-
2020
- 2020-10-13 CN CN202011092178.5A patent/CN114350486A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5494039A (en) * | 1993-07-16 | 1996-02-27 | Cryomedical Sciences, Inc. | Biopsy needle insertion guide and method of use in prostate cryosurgery |
| CN202562934U (en) * | 2012-05-18 | 2012-11-28 | 济南美医林电子仪器有限公司 | Full-automatic hemorrheology sample introduction system |
| CN104111344A (en) * | 2014-05-23 | 2014-10-22 | 苏州日和科技有限公司 | Fully automatic sample-injection mechanism of flow cytometer |
| CN206573356U (en) * | 2017-03-15 | 2017-10-20 | 中山众康医疗器械有限公司 | A kind of automatic Thinprep pap test pelleter sample-adding needle construction |
| CN208500977U (en) * | 2018-06-27 | 2019-02-15 | 武汉大学 | A kind of novel cell scoring devices |
| CN109799360A (en) * | 2019-01-09 | 2019-05-24 | 东软威特曼生物科技（沈阳）有限公司 | Automatic clinical chemistry analyzer and its sampling needle system |
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