CN114343581A - Quantitative monitoring system for transient dyskinesia - Google Patents
Quantitative monitoring system for transient dyskinesia Download PDFInfo
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- CN114343581A CN114343581A CN202210018076.1A CN202210018076A CN114343581A CN 114343581 A CN114343581 A CN 114343581A CN 202210018076 A CN202210018076 A CN 202210018076A CN 114343581 A CN114343581 A CN 114343581A
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Abstract
The invention discloses a quantitative monitoring system for transient dyskinesia, and relates to the technical field of sensing measurement. The system comprises: the device comprises a balance testing module, a gating control module and a balance processing module. The balance testing module comprises a balance beam, a balance motion experiment assembly and a flexible pressure sensor array; the gating control module comprises a row-column gating switch and an analog-digital conversion chip; the balance processing module comprises a main control chip and a visual terminal. Aiming at the problems that the existing animal balance test depends on manual realization, the balance criterion is insufficient and the transient dyskinesia of the animal cannot be judged, a brand new balance test module and a balance processing module are designed, the force criterion for introducing animal balance judgment and the animal balance posture visualization are realized, the mechanism that the original animal balance test only depends on manual scoring is optimized, the efficiency of animal balance analysis is improved, the judgment on the transient dyskinesia of the animal is completed, and the reliability of animal balance test data is improved.
Description
Technical Field
The invention belongs to the technical field of sensing measurement, and particularly relates to a quantitative monitoring system for monitoring transient dyskinesia of animal equilibrium/liveness.
Background
The vestibular organ and cerebellum are mainly used for regulating the movement of trunk muscles, maintaining the muscle tension and the body balance, and belong to the important movement regulation center in the central nervous system. The vestibular and cerebellum studies are often performed medically using animal balance experiments to study the balance ability of animals (e.g., mice).
In the animal balance experiment in the prior art, such as a balance beam experiment and a rotating rod experiment, the motion process of an animal is recorded by a camera, and the balance capability of the animal is determined only by manual observation and scoring, so that the analysis efficiency is reduced, and the accuracy of data obtained by the animal balance beam experiment can be reduced by simply depending on a manual mode to realize animal balance test along with the increase of experiment times and experiment time. Animal balance experiments based on manual observation and scoring also cannot accurately judge the state that the animal is reluctantly balanced on the balance beam and falls from the balance beam, and cannot quantify the scoring standard. Meanwhile, the existing balance beam experiment can not accurately reflect the participation degree of animals for keeping balance muscles, namely, the validity judgment of animal balance keeping behaviors based on force change can not be introduced, so that the experimental data lack certain persuasion.
In addition, brain damage, gene manipulation and drug therapy are major factors that currently lead to a decrease in the balance of the animal and even the development of dyskinesias. This impairment of motor ability is generally long-lasting or even permanent, so the balancing ability of the mice is constant at the time of the balancing experiment. For example, the influence of the influence factors represented by microwave irradiation on the animal balance capacity is transient, when the irradiation intensity level is low, the time for the animal to generate dyskinesia is greatly shortened, the irradiated animal can even be recovered to be normal in the balance experiment, and the traditional balance experiment cannot accurately judge the balance.
Therefore, the problem that the judgment function is incomplete due to the fact that the animal balance test depends on manual implementation and balance criteria are not enough needs to be solved.
Disclosure of Invention
In order to solve the problems, the invention discloses a quantitative monitoring system for transient dyskinesia, which optimizes the defect that the original animal balance test only depends on a manual scoring mechanism, designs a brand-new balance test module and a balance processing module, introduces a force criterion for judging the animal balance and visualizes the animal balance posture, improves the efficiency of animal balance analysis, finishes the judgment on the animal transient dyskinesia, and increases the reliability of animal balance test data.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the utility model provides a quantitative monitoring system of transient state dyskinesia, includes balanced test module, gating control module, balanced processing module, balanced test module includes balancing pole, balanced motion experiment subassembly, flexible pressure sensor array is connected in gating control module and the balanced test module, gating control module includes a plurality of ranks gating switch and a plurality of analog-to-digital conversion chips (ADC), balanced processing module includes main control chip and visual terminal, main control chip and gating control module among the balanced processing module are connected.
According to a preferred embodiment of the invention, the balance exercise experiment assembly comprises a darkroom and support frames for supporting balance rods, the balance rods are horizontally arranged, the number of the support frames is two, the support frames are respectively vertically arranged below two ends of the balance rods, the darkroom is arranged at the upper end of one of the support frames, and the darkroom is provided with an opening for the animal to go in and out.
According to a preferred embodiment of the present invention, the balanced exercise experimental assembly further comprises a carton disposed directly below the stabilizer bar for collecting animals dropped from the stabilizer bar.
According to a preferred embodiment of the present invention, the balance bar is cylindrical, the flexible pressure sensor array has a length equal to that of the balance bar and a width equal to the perimeter of the balance bar, and the flexible pressure sensor array is laid on the entire surface of the balance bar.
According to a preferred embodiment of the present invention, the flexible pressure sensor array is composed of n sets of square flexible pressure sensorsThe array is spliced, n groups of square flexible pressure sensor arrays are all arranged in p rows and p columns, the distance between the rows/columns is smaller than the distance between the animal claws, and the square flexible pressure sensor arrays are all provided with p2A plurality of sensitive points, the flexible pressure sensor array has np2Each sensitive point has an area not more than twice of the area of the animal claw, the gravity of the animal is q, the number of the claws is r, the maximum force applied by each claw is s, the measuring range of each sensitive point is 0-t, wherein t is t>(q/r)+s。
According to a preferred embodiment of the present invention, the row-column gating switches include row gating switches and column gating switches, which are m groups, each group of k row/column gating switches is a p-way 1-gating switch, and each group of row-column gating switches is respectively connected to each group of square flexible pressure sensor arrays to implement row-column gating of the sensing arrays.
According to a preferred embodiment of the present invention, the number of the ADC chips is m, the ADC chips are connected to the m groups of row and column gating switches, the ADC chip has q inputs, the ADC chip allows q analog inputs, and outputs a digital signal after analog-to-digital conversion, the resolution of the ADC chip is greater than or equal to 10 bits, so as to ensure the accuracy of the analog-to-digital conversion, and the sampling rate of the ADC chip is greater than 5 times of the maximum speed of animal movement, so as to ensure the integrity of sampling.
According to a preferred embodiment of the present invention, the main control chip controls the channel selection of the row-column gating switch, receives the sensor digital signal output by the ADC, and after processing, packages and transmits the measured pressure data to the visualization terminal.
According to a preferred embodiment of the invention, the visualization terminal visualizes the array pressure distribution according to the received data transmitted by the main control chip, and the posture of the animal on the balance beam and the participation degree of the animal for keeping balance muscles can be obtained through the array pressure distribution, so that the judgment of the animal balance based on the force criterion is completed.
The invention has the beneficial effects that:
the invention designs a brand new balance testing module and a balance processing module, realizes the visualization of the force criterion for introducing animal balance judgment and the animal balance posture, optimizes the mechanism that the original animal balance test only depends on manual scoring, improves the efficiency of animal balance analysis, and increases the reliability of animal balance test data.
The invention realizes the function of judging the state of the animal between reluctant balance keeping on the balance beam and falling off from the balance beam, and the pressure distribution diagram displayed by the visual terminal can reflect whether the animal is balanced on the balance beam and tries to keep balance, can reflect that the animal normally runs, the animal presses the belly on the balance beam for keeping balance, the animal surrounds the balance beam for keeping balance, the animal winds the balance beam for keeping balance tail in different postures, and the like, thereby refining the original scoring standard.
The invention realizes the judgment of the transient dyskinesia of the animal, and aims at the reduction of the animal balance caused by microwave irradiation, the traditional balance experiment misjudges the dyskinesia of the animal by dropping a balance rod of the animal, and the invention accurately judges whether the dyskinesia of the animal exists by combining the current pressure distribution and the historical data call, thereby correcting the misjudgment mechanism in the original balance experiment.
The flexible pressure sensor array designed by the invention is a splicing array, the size of the array and the number of sensitive points can be adjusted according to different balance experiment modes, different balance rods and different test animals, in addition, the number of input channels of the selected row-column gating switch can be changed according to different numbers of the rows and columns of the array, and the flexible pressure sensor array has good system structure flexibility.
Drawings
FIG. 1 is a block diagram of the present invention;
FIG. 2 is a schematic structural diagram of a balance test module according to the present invention;
FIG. 3 is a block diagram of a gating control module according to the present invention;
FIG. 4 is a block diagram of a balancing module according to the present invention;
fig. 5 is a graph of a pressure distribution map in a visualization terminal and a corresponding typical mouse posture in an embodiment of the invention.
List of reference symbols:
1. a support frame; 2. a balancing pole; 3. a flexible pressure sensor array; 4. a darkroom; 5. a carton.
Detailed Description
The present invention will be further illustrated with reference to the accompanying drawings and specific embodiments, which are to be understood as merely illustrative of the invention and not as limiting the scope of the invention.
As shown in fig. 1, the present invention discloses a quantitative monitoring system for transient dyskinesia, which comprises: the device comprises a balance testing module, a gating control module and a balance processing module.
As shown in fig. 2, the balance testing module comprises a balance bar 2, a balance motion experiment assembly, and a flexible pressure sensor array 3, wherein the balance motion experiment assembly, for example, a balance beam experiment assembly, comprises a dark room 4, a carton 5, and a support frame 1 for supporting the balance bar 2, the dark room 4 is disposed at the upper end of one of the support frames 1 and is provided with an opening for an animal to come in and go out, and the carton 5 is disposed directly below the balance bar 2. The flexible pressure sensor array 3 is laid on the whole surface of the balancing rod 2 and formed by splicing n groups of square flexible pressure sensor arrays, the n groups of square flexible pressure sensor arrays are all arranged in p rows and p columns, and the square flexible pressure sensor arrays are all provided with p2The flexible pressure sensor array formed by splicing the sensitive points has np2A sensitive point.
As shown in fig. 3, the gating module includes m × k row/column gating switches and m ADC chips. The row-column gating switch comprises m groups of row gating switches and column gating switches, each group of k row/column gating switches is a p-way 1-selecting switch and is respectively connected with p rows and p columns of the square flexible pressure sensor array, row-column gating of the sensing array is achieved, and sensing quantity is input into the ADC. The ADC chips are m in number and connected with m groups of row/column gating switches, the ADC chips allow q paths of analog quantity to be input, wherein q is larger than or equal to k, and digital signals are output after analog-to-digital conversion.
As shown in fig. 4, the balance processing module includes a main control chip and a visualization terminal. The main control chip controls the channel selection of the row-column gating switch, receives the sensor digital signals output by the ADC, and packages and transmits the measured pressure data to the visual terminal after processing.
The design of the embodiment verifies the feasibility of the self-adaptive system. In this embodiment, the balancing rod is a round stick with a length of 81cm and a diameter of 1.8 cm.
The square flexible pressure sensor array is a homemade flexible pressure sensor array based on CB/CNTs (carbon black-carbon nano tube composite material), the measuring range is 0-2N, the radius of a single sensitive point is 5mm, the distance between the sensitive points is 2mm, the number of rows/columns is 8, namely p is 8, the length/width of the array is 5.4cm, the fully-covered balancing pole is formed by splicing 15 square flexible pressure sensor arrays, namely N is 15.
The row/column gating switch adopts CD4051B chip of Texas instruments company, the chip realizes 8-channel analog multiplexing, the input analog quantity voltage range is-15V, and the output digital quantity voltage range is negative voltage VEEPower supply voltage VDDThe on-resistance is less than or equal to 125 omega, and the open circuit leakage current range is +/-100 pA.
The ADC chip is ADS5270 chip of Texas instruments, 8 input channels, 12-bit resolution and 40MSPS maximum sampling rate.
The main control chip selects an STM32F407 microcontroller of Italian semiconductor company, a kernel 32-bit ARM Cortex-M4 processor, provides 114 IO ports for controlling gating of a row-column switch and controlling work and data receiving of an ADC, and provides various interfaces such as USART and SPI which can communicate with a visual terminal.
The working flow of the embodiment is as follows: taking a mouse as an example, when the mouse crawls on a balancing pole, the sensing quantity output by sensitive points contacting with four claws, a body and a tail of the mouse changes, the sensing quantity is transmitted to an ADC chip ADS5270 after being conducted by a gate switch CD4051B which continuously traverses and scans, digital quantity is input to a main control chip STM32F407 after analog-to-digital conversion is completed, and the main control chip processes data and then packs the data to be sent to a visual terminal. The visualization terminal visualizes the array pressure distribution of the data, the posture of the animal on the balance beam and the participation degree of the animal for keeping balance muscles can be obtained through the array pressure distribution, and the judgment of the animal balance based on the force criterion is completed. The visualization terminal described in this embodiment is a desktop computer, which is convenient for programming to receive data and for data visualization. As shown in FIG. 5, the four images (a) (b) (c) (d) are respectively a typical posture and a corresponding visualized pressure distribution diagram of the mouse on the balance bar, and the direction of the dotted line in FIG. 5 is the same as the left-looking direction in FIG. 2. Fig. 5(a) is a pressure distribution diagram of a mouse normally running on a balancing pole, (b) is a pressure distribution diagram of a mouse abdomen pressed on the balancing pole, (c) is a pressure distribution diagram of the mouse encircling the balancing pole, and (d) is a pressure distribution diagram of a mouse tail wound on the balancing pole.
It should be noted that the above-mentioned contents only illustrate the technical idea of the present invention, and the protection scope of the present invention is not limited thereby, and it is obvious to those skilled in the art that several modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations fall within the protection scope of the claims of the present invention.
Claims (10)
1. A quantitative monitoring system of transient dyskinesia, comprising: the balance testing module comprises a balance rod, a balance motion experiment assembly and a flexible pressure sensor array, the gating control module comprises a plurality of row-column gating switches and a plurality of ADC (analog to digital converter) chips, and the balance processing module comprises a main control chip and a visual terminal.
2. The system for quantitative monitoring of transient dyskinesia as defined in claim 1, wherein: the input end of a row-column gating switch in the gating control module is connected with the output end of a flexible pressure sensor array in the balance test module, and the corresponding sensing points on the selection array of the row-column gating switch transmit data to the ADC chip.
3. The system for quantitative monitoring of transient dyskinesia as defined in claim 1, wherein: the main control chip in the balance processing module is respectively connected with the column-row gating switch and the ADC chip in the gating control module, and the main control chip controls corresponding pins of the column-row gating switch to realize array gating, receives and processes the sensor digital signals output by the corresponding ADC.
4. The system for quantitative monitoring of transient dyskinesia as defined in claim 1, wherein: the balance motion experiment assembly comprises a darkroom, a carton and support frames used for supporting the balance rods, the number of the support frames is two, the support frames are respectively vertically arranged below two ends of each balance rod, the carton is arranged under the balance rods, the darkroom is arranged at the upper end of one of the support frames, and the darkroom is provided with an opening for the entrance and exit of animals.
5. The system for quantitative monitoring of transient dyskinesia as defined in claim 1, wherein: the balance rod in the balance test module is cylindrical and horizontally arranged.
6. A quantitative monitoring system for transient dyskinesia as defined in claim 1 or claim 5, wherein: the length of the flexible pressure sensor array is equal to that of the balancing rod, the width of the flexible pressure sensor array is equal to the circumference of the balancing rod, and the flexible pressure sensor array is paved on the whole surface of the balancing rod.
7. The system for quantitative monitoring of transient dyskinesia as defined in claim 6, wherein: the flexible pressure sensor array is formed by splicing n groups of square flexible pressure sensor arrays, the n groups of square flexible pressure sensor arrays are all arranged in p rows and p columns, the distance between the rows and the columns is smaller than the distance between the animal claws, and the square flexible pressure sensor arrays are all provided with p2Each sensitive point has an area not more than twice of the area of the animal claw, the gravity of the animal is q, the number of the claws is r, the maximum force applied by each claw is s, the measuring range of each sensitive point is 0-t, wherein t is t>(q/r)+s。
8. The system for quantitative monitoring of transient dyskinesia as defined in claim 1, wherein: the row-column gating switch comprises m groups of row gating switches and column gating switches, each group of k row/column gating switches is a p-way 1-selecting switch, and each group of row-column gating switches is respectively connected with each group of square flexible pressure sensor arrays to realize row-column gating of the sensing arrays.
9. The system for quantitative monitoring of transient dyskinesia as defined in claim 1, wherein:
the number of the ADC chips is m, the ADC chips are connected with the m groups of row and column gating switches, the ADC chips are provided with q paths of input, wherein q is larger than or equal to k, q paths of analog quantity input are allowed by the ADC chips, digital signals are output after analog-to-digital conversion, the resolution of the ADC chips is larger than or equal to 10 bits, the analog-to-digital conversion precision is ensured, the sampling rate of the ADC chips is larger than 5 times of the maximum speed of animal movement, and the sampling integrity is ensured.
10. The use method of the quantitative monitoring system for transient dyskinesia as claimed in claim 1, wherein: taking a mouse as an example, when the mouse crawls on a balancing pole, the sensing quantity output by sensitive points in contact with four claws, a body and a tail of the mouse changes, the sensing quantity is transmitted to an ADC (analog to digital converter) chip after being conducted by a gating switch, a digital quantity is input to a main control chip after analog-to-digital conversion is finished, and the main control chip processes data and then packages the data and transmits the data to a visual terminal; the visualization terminal visualizes the array pressure distribution of the data, the posture of the animal on the balance beam and the participation degree of the animal for keeping balance muscles can be obtained through the array pressure distribution, and the judgment of the animal balance based on the force criterion is completed.
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| CN202210018076.1A CN114343581A (en) | 2022-01-07 | 2022-01-07 | Quantitative monitoring system for transient dyskinesia |
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