CN114341622A - 超分辨成像系统与方法、生物样品识别系统与方法、核酸测序成像系统与方法及核酸识别系统与方法 - Google Patents
超分辨成像系统与方法、生物样品识别系统与方法、核酸测序成像系统与方法及核酸识别系统与方法 Download PDFInfo
- Publication number
- CN114341622A CN114341622A CN202080062961.9A CN202080062961A CN114341622A CN 114341622 A CN114341622 A CN 114341622A CN 202080062961 A CN202080062961 A CN 202080062961A CN 114341622 A CN114341622 A CN 114341622A
- Authority
- CN
- China
- Prior art keywords
- light
- super
- structured light
- resolution imaging
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003384 imaging method Methods 0.000 title claims abstract description 181
- 239000012472 biological sample Substances 0.000 title claims abstract description 87
- 238000000034 method Methods 0.000 title claims abstract description 66
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 63
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 63
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 63
- 238000012163 sequencing technique Methods 0.000 title claims abstract description 36
- 238000005286 illumination Methods 0.000 claims abstract description 113
- 230000005284 excitation Effects 0.000 claims abstract description 81
- 239000000523 sample Substances 0.000 claims description 76
- 230000010287 polarization Effects 0.000 claims description 30
- 230000003287 optical effect Effects 0.000 claims description 21
- 230000000903 blocking effect Effects 0.000 claims description 8
- 230000003044 adaptive effect Effects 0.000 claims description 7
- 238000007493 shaping process Methods 0.000 claims description 3
- 102100029469 WD repeat and HMG-box DNA-binding protein 1 Human genes 0.000 claims 1
- 101710097421 WD repeat and HMG-box DNA-binding protein 1 Proteins 0.000 claims 1
- 230000000007 visual effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 11
- 238000010586 diagram Methods 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 9
- 238000000799 fluorescence microscopy Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 230000004907 flux Effects 0.000 description 3
- 238000012165 high-throughput sequencing Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/14—Condensers affording illumination for phase-contrast observation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
- G02B27/286—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising for controlling or changing the state of polarisation, e.g. transforming one polarisation state into another
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B2207/00—Coding scheme for general features or characteristics of optical elements and systems of subclass G02B, but not including elements and systems which would be classified in G02B6/00 and subgroups
- G02B2207/113—Fluorescence
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Multimedia (AREA)
- Biomedical Technology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Abstract
一种超分辨成像系统(1,41,51)、超分辨成像方法、生物样品识别系统(4,61)与方法、核酸测序成像系统(5)与方法及核酸识别系统(6)与方法，该超分辨成像系统(1,41,51)包括照明系统(A)与成像系统(B)，照明系统(A)输出激发光照射生物样品以生成受激光，成像系统(B)收集及记录受激光以生成受激光图像，照明系统(A)包括激发光源(10,10a)与结构光产生及调制装置(11,11a)，激发光源(10,10a)输出激发光，结构光产生及调制装置(11,11a)将激发光调制成结构光照射生物样品以产生受激光，结构光产生及调制装置(11,11a)包括结构光控制装置(113,113a)，结构光控制装置(113,113a)控制结构光产生及调制装置(11,11a)以改变其输出的结构光的相位与结构光投射于生物样品上的照明图案的方向，成像系统(B)配合结构光相位及/或照明图案的方向的改变拍摄至少一成像视场内至少一波长的激发光激发的受激光的图像，由此提升了成像效率。
Description
PCT国内申请，说明书已公开。
Claims (25)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2020/078444 WO2021179127A1 (zh) | 2020-03-09 | 2020-03-09 | 超分辨成像系统与方法、生物样品识别系统与方法、核酸测序成像系统与方法及核酸识别系统与方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114341622A true CN114341622A (zh) | 2022-04-12 |
Family
ID=77670357
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080062961.9A Pending CN114341622A (zh) | 2020-03-09 | 2020-03-09 | 超分辨成像系统与方法、生物样品识别系统与方法、核酸测序成像系统与方法及核酸识别系统与方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20240085717A1 (zh) |
| EP (1) | EP4024031A4 (zh) |
| CN (1) | CN114341622A (zh) |
| WO (1) | WO2021179127A1 (zh) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114460731B (zh) * | 2022-01-24 | 2023-03-17 | 浙江大学 | 一种基于dmd的多色结构光照明超分辨显微成像方法和装置 |
| CN114813673A (zh) * | 2022-04-12 | 2022-07-29 | 深圳赛陆医疗科技有限公司 | 多通道超分辨基因检测仪及其检测方法 |
| CN117368174A (zh) * | 2023-12-07 | 2024-01-09 | 深圳赛陆医疗科技有限公司 | 成像系统及成像方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10463256B2 (en) * | 2014-02-04 | 2019-11-05 | The Trustees Of Dartmouth College | Structured-light imaging systems and methods for determining sub-diffuse scattering parameters |
| CN104597590B (zh) * | 2014-12-30 | 2018-02-02 | 深圳先进技术研究院 | 一种超分辨荧光光谱成像显微镜 |
| WO2016125281A1 (ja) * | 2015-02-05 | 2016-08-11 | 株式会社ニコン | 構造化照明顕微鏡、観察方法、及び制御プログラム |
| WO2016199179A1 (ja) * | 2015-06-08 | 2016-12-15 | 株式会社ニコン | 構造化照明顕微鏡システム、方法及びプログラム |
| CN105589188B (zh) * | 2016-03-10 | 2018-01-16 | 清华大学 | 一种结构光照明显微镜的成像方法及装置 |
| US10330780B2 (en) * | 2017-03-20 | 2019-06-25 | Velodyne Lidar, Inc. | LIDAR based 3-D imaging with structured light and integrated illumination and detection |
| CN106979460A (zh) * | 2017-03-29 | 2017-07-25 | 中国科学院苏州生物医学工程技术研究所 | 一种荧光光源及荧光显微成像系统 |
| CN107389631B (zh) * | 2017-04-28 | 2020-07-07 | 中国科学院生物物理研究所 | 高速多色多模态结构光照明超分辨显微成像系统及其方法 |
| WO2020022721A1 (ko) * | 2018-07-24 | 2020-01-30 | 한국과학기술원 | 가변 구조광 생성 장치 및 3차원 이미징 시스템 |
| CN110141182A (zh) * | 2019-05-31 | 2019-08-20 | 苏州精观医疗科技有限公司 | 一种基于结构光照明的显微内窥成像方法及系统 |
| CN110515192B (zh) * | 2019-09-17 | 2024-04-30 | 苏州睿仟科技有限公司 | 基于水浸物镜的超分辨率快速自动扫描成像系统及方法 |
-
2020
- 2020-03-09 EP EP20924340.1A patent/EP4024031A4/en active Pending
- 2020-03-09 US US17/769,428 patent/US20240085717A1/en active Pending
- 2020-03-09 CN CN202080062961.9A patent/CN114341622A/zh active Pending
- 2020-03-09 WO PCT/CN2020/078444 patent/WO2021179127A1/zh active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| EP4024031A1 (en) | 2022-07-06 |
| EP4024031A4 (en) | 2023-05-31 |
| WO2021179127A1 (zh) | 2021-09-16 |
| US20240085717A1 (en) | 2024-03-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3145171B1 (en) | Chromatic aberration correction in imaging system including variable focal length lens | |
| CN114341622A (zh) | 超分辨成像系统与方法、生物样品识别系统与方法、核酸测序成像系统与方法及核酸识别系统与方法 | |
| CN108680544B (zh) | 一种结构化照明的光切片荧光显微成像方法和装置 | |
| US9930243B2 (en) | Variable focal length imaging system | |
| US10477097B2 (en) | Single-frame autofocusing using multi-LED illumination | |
| CN110262026B (zh) | 孔径扫描傅立叶重叠关联成像 | |
| CA2079882C (en) | Confocal microscope | |
| CN109445089B (zh) | 一种基于高速波前调制的多模光纤三维成像装置及方法 | |
| US20050146784A1 (en) | Confocal microscope having multiple independent excitation paths | |
| CN114813673A (zh) | 多通道超分辨基因检测仪及其检测方法 | |
| US8570625B2 (en) | Device and method for generating an image of an object | |
| CN105929560A (zh) | 一种宽带远场超分辨成像装置 | |
| CN109900671B (zh) | 基于dmd计算全息扫描的全自动化tcspc-flim系统和时间检测方法 | |
| CN107202780B (zh) | 一种基于散斑照明的超分辨显微方法和装置 | |
| CN111175259A (zh) | 具有结构化照明的三维显微术的加速的方法和设备 | |
| CN116507956A (zh) | 利用扫描镜和转移工作台的飞越光束图案扫描全息图显微镜装置 | |
| CN109870441B (zh) | 基于移频的三维超分辨光切片荧光显微成像方法和装置 | |
| US20130250088A1 (en) | Multi-color confocal microscope and imaging methods | |
| JP2005292538A (ja) | 走査型光学顕微鏡 | |
| WO2009066252A2 (en) | Multi-focal spot generator and multi-focal multi-spot scanning microscope | |
| US9772540B2 (en) | Autofocus for imaging system | |
| JP2007278849A (ja) | 光学測定装置及び光学測定方法 | |
| RU2579640C1 (ru) | Конфокальный спектроанализатор изображений | |
| JP2005164815A (ja) | 光学装置 | |
| KR20010034046A (ko) | 다수의 스캐닝 빔을 갖는 공초점 현미경 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 40065762 Country of ref document: HK |