CN114324555A - Isotope detection method - Google Patents
Isotope detection method Download PDFInfo
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- CN114324555A CN114324555A CN202111660012.3A CN202111660012A CN114324555A CN 114324555 A CN114324555 A CN 114324555A CN 202111660012 A CN202111660012 A CN 202111660012A CN 114324555 A CN114324555 A CN 114324555A
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- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000013507 mapping Methods 0.000 claims abstract description 14
- 238000012545 processing Methods 0.000 claims abstract description 5
- 239000003085 diluting agent Substances 0.000 claims description 10
- 238000005086 pumping Methods 0.000 claims description 7
- 238000004458 analytical method Methods 0.000 claims description 2
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 claims 1
- 238000000034 method Methods 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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Abstract
The invention provides an isotope detection method, which comprises the following steps: after the sample is quantified, the sample is sent to an analyzer for detection to obtain the first isotope MxCorresponding first signal AxAnd with a second isotope MyCorresponding second signal Ay(ii) a Processing the first signal by using a mapping relation to obtain the concentration C of the first isotope in the samplex(ii) a Preparing standard liquid by utilizing isotope marking liquid, wherein the content of a first isotope in the standard liquid is Cx(ii) a The standard liquid is sent to the analyzer to obtain a first isotope MxCorresponding third signal A'xAnd with a second isotope MyCorresponding fourth signal A'y(ii) a According to signal Ax、Ay、A′x、A′yAnd obtaining the isotope ratio in the sample. The invention has the advantages of self-adaptation and the like.
Description
Technical Field
The present invention relates to isotopes, and in particular to methods of isotope detection.
Background
In isotope ratio determination, the instrument used is an inductively coupled plasma mass spectrometer (ICP-MS), and the measurement is usually performed by a sample-standard intersection method, i.e., the measurement of a standard is performed after the sample test to correct the mass discrimination of the instrument, and the isotope ratio determination result is expressed in the thousandth deviation of the sample from the standard. Therefore, the concentration of the standard sample and the concentration of the sample must be kept close to each other during the experiment, so as to eliminate the isotope fractionation phenomenon caused by the concentration difference.
The current method comprises the following steps: the accurate concentration of the M element is measured firstly, and then the M element is diluted to the same level according to different sample concentrations.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides an isotope detection method.
The purpose of the invention is realized by the following technical scheme:
an isotope detection method, comprising:
after the sample is quantified, the sample is sent to an analyzer for detection to obtain the first isotope MxCorresponding first signal AxAnd with a second isotope MyCorresponding second signal Ay；
Processing the first signal by using a mapping relation to obtain the concentration C of the first isotope in the samplex；
Preparing standard liquid by utilizing isotope marking liquid, wherein the content of a first isotope in the standard liquid is Cx；
The standard liquid is sent to the analyzer to obtain a first isotope MxCorresponding third signal A'xAnd with a second isotope MyCorresponding fourth signal A'y；
According to signal Ax、Ay、A′x、A′yAnd obtaining the isotope ratio in the sample.
Compared with the prior art, the invention has the beneficial effects that:
1. self-adapting;
obtaining the isotope concentration of the sample according to the established mapping relation and the analysis of the sample; the standard liquid with the same isotope concentration as the sample is automatically prepared by utilizing the standard liquid, and the self-adaptive function is realized;
2. the detection result is accurate;
the invention adopts the combination of the pipeline, the multi-way valve and the injection pump, not only accurately quantifies the sample and the standard solution, but also can clean the pipeline and the quantifying ring and prevent the cross contamination among the samples.
Drawings
The disclosure of the present invention will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only for illustrating the technical solutions of the present invention and are not intended to limit the scope of the present invention. In the figure:
fig. 1 is a schematic flow diagram of an isotope detection method according to an embodiment of the present invention.
Detailed Description
Fig. 1 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. Some conventional aspects have been simplified or omitted for the purpose of explaining the technical solution of the present invention. Those skilled in the art will appreciate that variations or substitutions from these embodiments will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
as shown in fig. 1, the isotope detection method according to the embodiment of the present invention includes:
after the sample is quantified, the sample is sent to an analyzer for detection to obtain the first isotope MxCorresponding to the first signal AxAnd with a second isotope MyCorresponding second signal Ay；
Processing the first signal by using a mapping relation to obtain the concentration C of the first isotope in the samplex；
Preparing standard liquid by utilizing isotope marking liquid, wherein the content of a first isotope in the standard liquid is Cx；
The labelThe quasi-liquid is sent to the analyzer to obtain the first isotope MxCorresponding third signal A'xAnd with a second isotope MyCorresponding fourth signal A'y；
According to signal Ax、Ay、A′x、A′yAnd obtaining the isotope ratio in the sample.
In order to accurately obtain the mapping relationship, the obtaining method of the mapping relationship further includes:
and sending the standard liquid with different concentrations to the analyzer, obtaining the mapping relation between the concentration and the signal value according to the obtained signal value and the concentration of the standard liquid, and storing the mapping relation.
For automation and accurate quantification, further, the sample quantification mode is as follows:
and switching the multi-way valve, pumping by the second pump, and enabling the sample to sequentially pass through the port of the multi-way valve, the quantitative ring and the second pump.
In order to automatically and accurately prepare the standard liquid, further, the preparation method of the standard liquid is as follows:
switching the multi-way valve, pumping by a second pump, and allowing the standard liquid to enter a pipeline; simultaneously, the first pump pushes out the diluent and gets into the pipeline, and the mark liquid mixes with the diluent to pass through port, ration ring and the second pump of multi-way valve in proper order.
In order to automatically and accurately prepare the standard liquid, the working parameters of the first pump and the second pump meet the following conditions:
Example 2:
an application example of the isotope detection method according to example 1 of the present invention.
In the application example, one end of the pipeline is suitable for inputting a diluent or a sample, and the other end of the pipeline is communicated with a first port of a multi-way valve (a six-way valve); the first pump and the second pump both adopt injection pumps, and the capacity of the second pump is larger than that of the first pump; the output of the first pump is communicated with the pipeline, and the input of the second pump is communicated with the sixth port of the multi-way valve; the multi-way valve adopts a six-way valve, two ends of the quantitative ring are respectively communicated with the second port and the fifth port, the third port is communicated with the ICP-MS, and the fourth port is communicated with the carrier liquid.
As shown in fig. 1, the isotope detection method according to the embodiment of the present invention includes:
pumping by a second pump, switching by a multi-way valve, conveying the standard liquid with different concentrations to a quantitative ring, conveying the standard liquid to ICP-MS by using carrier liquid, outputting a signal value by the ICP-MS, and obtaining a mapping relation C between the isotope concentration of the (standard liquid) and the signal valuex＝f(Ax) And storing;
switching the multi-way valve, pumping by a second pump, and enabling the sample to sequentially pass through a port of the multi-way valve, the quantitative ring and the second pump; then, the multi-way valve is switched, the carrier liquid carries the sample in the quantitative ring to send to the analyzer, and a first signal A corresponding to the first isotope Mx is obtainedxAnd with a second isotope MyCorresponding second signal Ay；
Using the mapping relation Cx＝f(Ax) Processing said first signal to obtain a concentration C of a first isotope in said samplex；
Preparing standard liquid by utilizing isotope marking liquid, wherein the content of a first isotope in the standard liquid is Cx(ii) a The preparation method comprises the following steps: switching the multi-way valve, pumping by a second pump, and allowing the standard liquid to enter a pipeline; meanwhile, the first pump pushes out diluent to enter the pipeline, the standard solution and the diluent are mixed and sequentially pass through a port of the multi-way valve, a quantitative ring and the second pump; the working parameters of the first pump and the second pump meet the following conditions:
switching the multi-way valve, and sending the standard liquid carried by the carrier liquid to the analyzer to obtain the first same positionElement MxCorresponding third signal A'xAnd with a second isotope MyCorresponding fourth signal A'y；
According to signal Ax、Ay、A′x、A′yThe isotopic ratios in the samples are obtained and calculated in a manner known in the art.
Claims (7)
1. An isotope detection method, comprising:
after the sample is quantified, the sample is sent to an analyzer for detection to obtain the first isotope MxCorresponding first signal AxAnd with a second isotope MyCorresponding second signal Ay；
Processing the first signal by using a mapping relation to obtain the concentration C of the first isotope in the samplex；
Preparing standard liquid by utilizing isotope marking liquid, wherein the content of a first isotope in the standard liquid is Cx；
The standard liquid is sent to the analyzer to obtain a first isotope MxCorresponding third signal A'xAnd with a second isotope MyCorresponding fourth signal A'y；
According to signal Ax、Ay、A′x、A′yAnd obtaining the isotope ratio in the sample.
2. The isotope detection method according to claim 1, wherein the mapping relationship is obtained by:
and sending the standard liquid with different concentrations to the analyzer, obtaining the mapping relation between the concentration and the signal value according to the obtained signal value and the concentration of the standard liquid, and storing the mapping relation.
3. The isotope detection method according to claim 1, wherein the sample is quantified by:
and switching the multi-way valve, pumping by the second pump, and enabling the sample to sequentially pass through the port of the multi-way valve, the quantitative ring and the second pump.
4. The isotope detection method according to claim 1, wherein the standard liquid is prepared in such a manner that:
switching the multi-way valve, pumping by a second pump, and allowing the standard liquid to enter a pipeline; simultaneously, the first pump pushes out the diluent and gets into the pipeline, and the mark liquid mixes with the diluent to pass through port, ration ring and the second pump of multi-way valve in proper order.
5. An isotope detection method in accordance with claim 4, wherein the operating parameters of the first and second pumps are such that:
6. The isotope detection method in accordance with claim 4, wherein the first pump and the second pump employ syringe pumps.
7. The isotope detection method in accordance with claim 1, wherein the analysis is ICP-MS.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111660012.3A CN114324555A (en) | 2021-12-30 | 2021-12-30 | Isotope detection method |
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| CN202111660012.3A CN114324555A (en) | 2021-12-30 | 2021-12-30 | Isotope detection method |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07306183A (en) * | 1994-05-12 | 1995-11-21 | Mitsubishi Heavy Ind Ltd | Hydrogen isotope ratio analyzing method |
| CN103983715A (en) * | 2014-04-08 | 2014-08-13 | 中国原子能科学研究院 | TIMS measuring method for oxygen isotope ratio in uranium oxide |
| US20190128860A1 (en) * | 2016-04-05 | 2019-05-02 | Perkinelmer Japan Co., Ltd. | Analysis method and analysis device for substance to be measured |
| CN110823989A (en) * | 2018-08-07 | 2020-02-21 | 塞莫费雪科学（不来梅）有限公司 | Isotope ratio measurement |
| CN111999373A (en) * | 2020-08-07 | 2020-11-27 | 中国科学院生态环境研究中心 | Method for detecting abundance ratio of copper isotopes in blood and application of method as bladder cancer marker |
| CN112730581A (en) * | 2021-01-18 | 2021-04-30 | 天津师范大学 | Sulfur monoxide ion method for detecting sulfur stable isotope in atmosphere |
| CN113295675A (en) * | 2021-05-10 | 2021-08-24 | 中国科学院合肥物质科学研究院 | Novel uranium isotope ratio measuring device and method thereof |
-
2021
- 2021-12-30 CN CN202111660012.3A patent/CN114324555A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07306183A (en) * | 1994-05-12 | 1995-11-21 | Mitsubishi Heavy Ind Ltd | Hydrogen isotope ratio analyzing method |
| CN103983715A (en) * | 2014-04-08 | 2014-08-13 | 中国原子能科学研究院 | TIMS measuring method for oxygen isotope ratio in uranium oxide |
| US20190128860A1 (en) * | 2016-04-05 | 2019-05-02 | Perkinelmer Japan Co., Ltd. | Analysis method and analysis device for substance to be measured |
| CN110823989A (en) * | 2018-08-07 | 2020-02-21 | 塞莫费雪科学（不来梅）有限公司 | Isotope ratio measurement |
| CN111999373A (en) * | 2020-08-07 | 2020-11-27 | 中国科学院生态环境研究中心 | Method for detecting abundance ratio of copper isotopes in blood and application of method as bladder cancer marker |
| CN112730581A (en) * | 2021-01-18 | 2021-04-30 | 天津师范大学 | Sulfur monoxide ion method for detecting sulfur stable isotope in atmosphere |
| CN113295675A (en) * | 2021-05-10 | 2021-08-24 | 中国科学院合肥物质科学研究院 | Novel uranium isotope ratio measuring device and method thereof |
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