CN114323900A - Quick preparation facilities of biological sample tissue free water tritium - Google Patents
Quick preparation facilities of biological sample tissue free water tritium Download PDFInfo
- Publication number
- CN114323900A CN114323900A CN202011056094.6A CN202011056094A CN114323900A CN 114323900 A CN114323900 A CN 114323900A CN 202011056094 A CN202011056094 A CN 202011056094A CN 114323900 A CN114323900 A CN 114323900A
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- Prior art keywords
- free water
- tritium
- condensing
- water tritium
- constant volume
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 42
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 title claims abstract description 35
- 229910052722 tritium Inorganic materials 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000012472 biological sample Substances 0.000 title claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 21
- 238000007664 blowing Methods 0.000 claims abstract description 18
- 239000000523 sample Substances 0.000 claims abstract description 17
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 238000005265 energy consumption Methods 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000004108 freeze drying Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 239000010705 motor oil Substances 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 4
- 238000009833 condensation Methods 0.000 description 3
- 230000005494 condensation Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 240000008100 Brassica rapa Species 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 230000005653 Brownian motion process Effects 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000010533 azeotropic distillation Methods 0.000 description 1
- 238000005537 brownian motion Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Measurement Of Radiation (AREA)
Abstract
The invention discloses a device for quickly preparing free water tritium of a biological sample tissue, which comprises a heating device, a constant volume device, a rotating device, a condensing device, a vacuum device and an air blowing device, wherein the rotating device, the heating device and the condensing device are connected, and the constant volume device, the air blowing device and the vacuum device are connected with the condensing device. The invention has the beneficial effects that: the results of the preparation of tissue free water tritium by adopting a freeze dryer method and the preparation of tissue free water tritium by adopting the device show that the sample prepared by the device and the sample prepared by the freeze drying method have good parallelism, the data are reliable, and the most important is that the device has low cost and is convenient to popularize. The freeze dryer needs to be started for a long time, the energy consumption is high, and engine oil needs to be replaced regularly. The device does not have consumptive material expense usually, has saved experiment operating personnel's operating time, greatly reduced the energy consumption, and can avoid the emergence of burning and scalding the risk.
Description
Technical Field
The invention belongs to a preparation device, and particularly relates to a device for rapidly preparing free water tritium of a biological sample tissue.
Background
In China, places such as operating nuclear power stations, researching reactors, tritium target production and the like all relate to tritium operation. Tritium enters an ecological system through transpiration, molecular brownian motion and surface runoff falling on plant leaves and being absorbed fundamentally to generate internal irradiation on organisms, so that the organisms are damaged. At present, when a tissue free water tritium sample is prepared in domestic power stations and scientific research institutions, the tissue free water tritium sample is mainly prepared by adopting a freeze dryer, vacuum freeze drying and azeotropic distillation. The methods have the disadvantages of expensive equipment, high energy consumption, long time consumption and burning risk, and a safe and reliable device with high cost performance and low energy consumption is urgently needed to be developed to supplement the original device.
Disclosure of Invention
The invention aims to provide a device for quickly preparing free water tritium in tissues of a biological sample, which can realize quick and efficient preparation of the free water tritium in the tissues of the biological sample, save time and achieve intrinsic safety.
The technical scheme of the invention is as follows: biological sample organizes quick preparation facilities of free water tritium, it includes heating device, constant volume device, rotary device, condensing equipment, vacuum device and air-blowing device, rotary device be connected with condensing equipment, heating device heats rotary device, constant volume device, air-blowing device and vacuum device be connected with condensing equipment. When the sample is normally prepared, the air blowing device stops working, and the rotating device, the condensing device, the vacuum device and the constant volume device form a closed system; when the sample is not prepared, the system is opened, and an air blowing device is utilized to blow the adherent tritium sample.
The heating device is controllable in temperature, and the temperature can be adjusted (0-200 ℃) as required.
The sample adding port of the heating device adopts a frosted port with the diameter of 8 cm.
The constant volume device is provided with scales (0-20 mL, 0-50 mL, 0-100 mL and 3 constant volume ranges).
The rotating device is controllable in rotating speed, and the rotating speed (0-300 rpm) can be adjusted according to needs.
The temperature of the condensing device is controllable, and the temperature can be adjusted to (-26-30 ℃) as required.
The air blowing device is controllable in temperature, distilled water can be sucked in according to needs to clean the wall of the condensing pipe, and the air speed (0-10 m/s) and the temperature (0-60 ℃) can be adjusted.
The invention has the beneficial effects that: a comparative experiment of the freeze drying method and the tissue free water tritium prepared by the device is carried out in a laboratory. Tissue free water tritium of 6 media commonly used in laboratories is prepared by utilizing a freeze drying method and the device, and the determination results are as follows:
the specific test conditions are as follows:
BRADFORD is a qualitative detection kit for proteins. Through qualitative test of a sample prepared from milk, the result shows that the BRADFORD developer can not be subjected to color reaction with the sample prepared by a freeze dryer, which indicates that the rotary heating of the device can not carry out protein carrying and can not interfere with the experiment.
The results of the preparation of tissue free water tritium by adopting a freeze dryer method and the preparation of tissue free water tritium by adopting the device show that the sample prepared by the device and the sample prepared by the freeze drying method have good parallelism, the data are reliable, and the most important is that the device has low cost and is convenient to popularize. The freeze dryer needs to be started for a long time, the energy consumption is high, and engine oil needs to be replaced regularly. The device does not have consumptive material expense usually, has saved experiment operating personnel's operating time, greatly reduced the energy consumption, and can avoid the emergence of burning and scalding the risk.
Drawings
FIG. 1 is a schematic diagram of a device for rapidly preparing free water tritium from a tissue of a biological sample provided by the invention;
FIG. 2 is a schematic diagram illustrating a normal operation state of the present invention;
FIG. 3 is a schematic diagram of the water washing and air blowing process to remove condensed water on the wall of the condenser tube after the preparation is completed.
In the figure, 1 a heating device, 2 a constant volume device, 3 a rotating device, 4 a condensing device, 5 a vacuum device and 6 an air blowing device.
Detailed Description
The invention is described in further detail below with reference to the figures and the embodiments.
The invention integrates the electric motor, the heating pot, the condenser pipe, the vacuum pump, the water washing air-blowing drying and the quantitative container into a whole, thereby realizing the rapid preparation of the free water tritium of the biological sample tissue.
The device of the invention has two states, one is a normal working state as shown in figure 2, and the other is a state that condensed water on the wall of the condensing tube is removed by water washing and air blowing after the preparation is finished as shown in figure 3.
As shown in figure 1, the device for rapidly preparing free water tritium of biological sample tissue comprises a heating device 1, a constant volume device 2, a rotating device 3, a condensing device 4, a vacuum device 5 and an air blowing device 6. Wherein, the rotating device 6, the heating device 1 are connected with the condensing device 4, the constant volume device 2, the air blowing device 6 and the vacuum device 5 are connected with the condensing device 4.
Wherein, the heating device 1: place biological appearance in heating device, this heating device temperature is controllable, can adjust the temperature as required (0 ~ 200 ℃), selects 70 ℃ during biological appearance tissue free water tritium preparation, and the sample addition mouth adopts the dull polish mouth of diameter 8cm, is convenient for install jumbo size solid sample (meat, turnip, green vegetable, tealeaves etc.) additional, and convenient the dismantling is conveniently washd simultaneously. A safety clamp is added on the frosted opening to prevent the frosted opening from falling;
a constant volume device 2: the volume fixing device is provided with scales (0-20 mL), the tissue free water tritium of about 8mL is needed for one-time sample determination, and the machine can be stopped when the determination requirement is met according to the actual detection requirement;
the rotating device 3: the rotating device is controllable in rotating speed, the rotating speed (0-300 rpm) can be adjusted as required, and the rotating speed is 150rpm/min during biological sample preparation;
the condensing device 4: the temperature of a condensing device is controllable, an antifreezing solution is added into a condensing tank, the temperature can be adjusted to be (-26-30 ℃) as required, and 4 ℃ is selected when the free water tritium of the biological sample tissue is prepared;
a vacuum device 5: in order to ensure the air tightness of the device, vaseline is coated on the joint at the beginning of use until the vacuum gauge shows that the pressure is-0.1 MPa. After all the samples are normal, about 20 minutes of tissue free water tritium flows into the constant volume device, about 1 hour of the samples can be prepared, 8mL of tissue free water tritium in the constant volume device is directly transferred, and scintillation liquid is added to carry out determination work on a detection instrument.
And (3) an air blowing device 6: the air blowing device is controllable in temperature, distilled water can be sucked in according to needs to clean the wall of the condensation pipe, the air speed (0-10 m/s) and the temperature (0-60 ℃) can be adjusted, and water vapor on the wall of the condensation pipe is blown until the wall of the condensation pipe is free of liquid and hung on the wall for the next use.
The device aims at saving the operating time of experimenters and avoiding the generation of scalding risks in the experimental process. The evaporation of whole device through heating reinforcing moisture can change the area of contact between heating utensil and the heating device through going up and down, through the contact specific surface area of rotatory increase sample, bleeds through the vacuum pump and changes the traffic direction of steam, condenses into water with steam through condensing equipment, collects water through the ration container, blows the device through washing gas and realizes that the wall washs and the drying.
The device is specially used for extracting free water tritium from biological sample tissue. The equipment also has the capacity of extracting volatile liquid through extension expansion, and can realize liquid-liquid separation and solid-liquid separation. The device has guiding significance for preparing total beta radioactive samples in nuclear power plant effluents.
Claims (7)
1. Quick preparation facilities of biological sample tissue free water tritium which characterized in that: the device comprises a heating device, a constant volume device, a rotating device, a condensing device, a vacuum device and an air blowing device, wherein the rotating device, the heating device and the condensing device are connected, and the constant volume device, the air blowing device and the vacuum device are connected with the condensing device.
2. A device for the rapid preparation of free water tritium in biological samples according to claim 1, characterized in that: the heating device is controllable in temperature, and the temperature can be adjusted (0-200 ℃) as required.
3. A device for the rapid preparation of free water tritium in biological samples according to claim 1, characterized in that: the sample adding port of the heating device adopts a frosted port with the diameter of 8 cm.
4. A device for the rapid preparation of free water tritium in biological samples according to claim 1, characterized in that: the constant volume device is provided with scales (0-20 mL, 0-50 mL, 0-100 mL and 3 constant volume ranges).
5. A device for the rapid preparation of free water tritium in biological samples according to claim 1, characterized in that: the rotating device is controllable in rotating speed, and the rotating speed (0-300 rpm) can be adjusted according to needs.
6. A device for the rapid preparation of free water tritium in biological samples according to claim 1, characterized in that: the temperature of the condensing device is controllable.
7. A device for the rapid preparation of free water tritium in biological samples according to claim 1, characterized in that: the air blowing device is controllable in temperature, distilled water can be sucked in according to needs to clean the wall of the condensing pipe, and the air speed (0-10 m/s) and the temperature (0-60 ℃) can be adjusted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011056094.6A CN114323900A (en) | 2020-09-30 | 2020-09-30 | Quick preparation facilities of biological sample tissue free water tritium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011056094.6A CN114323900A (en) | 2020-09-30 | 2020-09-30 | Quick preparation facilities of biological sample tissue free water tritium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114323900A true CN114323900A (en) | 2022-04-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011056094.6A Pending CN114323900A (en) | 2020-09-30 | 2020-09-30 | Quick preparation facilities of biological sample tissue free water tritium |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4445037A (en) * | 1981-01-27 | 1984-04-24 | The United States Of America As Represented By The United States Department Of Energy | Apparatus for monitoring tritium in tritium contaminating environments using a modified Kanne chamber |
| JPH0552723A (en) * | 1991-08-23 | 1993-03-02 | Sci Service Kk | Apparatus and method for testing macro scopic structure of metallic material |
| JP2004271200A (en) * | 2003-03-05 | 2004-09-30 | Kawasaki Heavy Ind Ltd | Decontamination method and device for tritium-contaminated metal |
| US20090301228A1 (en) * | 2006-07-04 | 2009-12-10 | Yves Baron | Method for automatically sampling tritium in the water vapor in air |
| CN206523358U (en) * | 2017-03-14 | 2017-09-26 | 山西农业大学 | A kind of extraction element of free water tritium |
| CN110208042A (en) * | 2019-06-04 | 2019-09-06 | 中国工程物理研究院核物理与化学研究所 | It is a kind of biology sample in tissue free water tritium sampler |
| CN213985932U (en) * | 2020-09-30 | 2021-08-17 | 中核核电运行管理有限公司 | Quick preparation facilities of biological sample tissue free water tritium |
-
2020
- 2020-09-30 CN CN202011056094.6A patent/CN114323900A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4445037A (en) * | 1981-01-27 | 1984-04-24 | The United States Of America As Represented By The United States Department Of Energy | Apparatus for monitoring tritium in tritium contaminating environments using a modified Kanne chamber |
| JPH0552723A (en) * | 1991-08-23 | 1993-03-02 | Sci Service Kk | Apparatus and method for testing macro scopic structure of metallic material |
| JP2004271200A (en) * | 2003-03-05 | 2004-09-30 | Kawasaki Heavy Ind Ltd | Decontamination method and device for tritium-contaminated metal |
| US20090301228A1 (en) * | 2006-07-04 | 2009-12-10 | Yves Baron | Method for automatically sampling tritium in the water vapor in air |
| CN206523358U (en) * | 2017-03-14 | 2017-09-26 | 山西农业大学 | A kind of extraction element of free water tritium |
| CN110208042A (en) * | 2019-06-04 | 2019-09-06 | 中国工程物理研究院核物理与化学研究所 | It is a kind of biology sample in tissue free water tritium sampler |
| CN213985932U (en) * | 2020-09-30 | 2021-08-17 | 中核核电运行管理有限公司 | Quick preparation facilities of biological sample tissue free water tritium |
Non-Patent Citations (1)
| Title |
|---|
| 康厚军;张东;石正坤;: "植物中组织自由水氚的测量方法", 原子能科学技术, no. 03, 20 March 2009 (2009-03-20) * |
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