CN114317346B - Klebsiella strain capable of generating object surfactant and degradable benzene series and application thereof - Google Patents
Klebsiella strain capable of generating object surfactant and degradable benzene series and application thereof Download PDFInfo
- Publication number
- CN114317346B CN114317346B CN202111628837.7A CN202111628837A CN114317346B CN 114317346 B CN114317346 B CN 114317346B CN 202111628837 A CN202111628837 A CN 202111628837A CN 114317346 B CN114317346 B CN 114317346B
- Authority
- CN
- China
- Prior art keywords
- klebsiella
- paraxylene
- surfactant
- strain
- degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention discloses a self-produced surfactant and a Klebsiella strain of degradable benzene series and application thereof. The strain is named Klebsiella sp.HN02 and is deposited in the Guangdong institute of microbiology (GDMCC) with the deposit number of GDMCC No:62011, the date of preservation is 2021, 11 and 4. The strain is Klebsiella bacteria, is gram negative, has a short rod shape and is partially approximately spherical. Culturing on NA plate for 2 days, and has circular colony, raised surface, smooth surface and regular edge. The klebsiella HN02 can be used for environmental remediation, and the strain can take paraxylene as carbon to be derived from glycolipid-producing biosurfactant, so that the gas-liquid two-phase mass transfer rate in a system is improved, the paraxylene which is an environmental pollutant is degraded, the degradation efficiency is very high, 100% degradation of 21mg/L of paraxylene is realized in 72 hours, and 100% degradation of 26mg/L of paraxylene is realized in 120 hours.
Description
Technical Field
The invention relates to the technical field of microorganisms and environmental remediation, in particular to a Klebsiella strain capable of generating a biological surfactant and a degradable benzene series and application thereof.
Background
Para-xylene (PX) is a typical Volatile Organic Compound (VOC). It is an isomer of xylene. Mainly for the production of terephthalic acid (TPA) and as a main precursor for the production of polyethylene terephthalate, widely used in the production of plastics, films and synthetic fibers. Taking china as an example, even if a large amount of paraxylene is produced annually, tens of millions of tons of paraxylene still need to be imported to meet the industrial production needs. Paraxylene is easily vaporized and thus often enters the air, rarely exists in high concentrations in surface water or earth, and also causes air pollution problems to some extent. Paraxylene is toxic, volatile, insoluble in water, and easy to directly contact with human body, thereby causing damage to skin, eyes, respiratory system and organs of human body. In addition, xylene has extremely low odor threshold, has strong odor at a low concentration, and can have adverse effects on physiological and psychological health of people. Therefore, efficient removal of paraxylene from the atmosphere is a very interesting topic. But paraxylene is poorly soluble in water and therefore its utilization is also generally low, so it is very necessary and urgent to increase the solubility of paraxylene and to effectively remove paraxylene from the environment. The surfactant has amphipathy, so that the surfactant can improve the solubility of certain insoluble or indissolvable substances in water, is often used for improving the solubility of indissolvable substances to assist in use, but the traditional chemical surfactant has a complex structure and certain secondary toxicity, while the method for degrading organic pollutants in the environment by utilizing microorganisms is an environment-friendly method, and certain microorganisms can also produce environment-friendly and biodegradable natural biosurfactants, so that the separation and screening of bacteria capable of degrading paraxylene and simultaneously producing biosurfactants is a very good method for solving the problem of degrading paraxylene, and therefore, the screening of bacteria capable of degrading paraxylene at low cost and high efficiency and simultaneously producing the biosurfactants from has very important significance in the aspect of purifying malodorous organic waste gas.
Klebsiella is a gram-negative bacterium, which is widely distributed in nature and can be detected in water, soil, food, animal respiratory tract and intestinal tract. Li Tao et al (2009) in "screening of biosurfactant strains and preliminary optimization of fermentation conditions" indicate: a biosurfactant-producing strain was isolated from soil samples collected from the canteen of university of Hainan (Methoff) and designated S423. The strain was determined to belong to the genus Klebsiella by 16S rDNA phylogenetic analysis, and the active substance was initially identified as glycolipid by qualitative analysis, thin Layer Chromatography (TLC) and infrared spectrophotometry (IR) analysis. It can be seen that the Klebsiella isolated from the resultant surfactant has been studied. However, so far, no related study or report has been made on the fact that klebsiella can degrade gaseous paraxylene while self-generating a surfactant to again improve the degradation efficiency of gaseous hydrophobic paraxylene.
Disclosure of Invention
In view of the shortcomings of the prior art, it is an object of the present invention to provide a Klebsiella strain that produces a surfactant and a degradable benzene-based material. The Klebsiella HN02 belongs to a new variety of Klebsiella, has excellent paraxylene degradation capability, simultaneously generates a self-generated surfactant, can degrade paraxylene with higher concentration in the environment, and has high degradation rate.
The homology of the strain 16SrDNA sequence of the invention with Klebsiella pneumoniae strain MGH83 was found to be 99.93% by 16S: rDNA sequence comparison analysis. The novel variety of the Klebsiella (Klebsiella) HN02 belongs to the genus Klebsiella by combining the characteristics of the bacterial form, the growth condition and the physiological and biochemical identification result.
The Klebsiella (Klebsiella) HN02 is currently deposited in the Guangdong institute of microbiology (GDMCC) with a deposition address of No. 59 building 5 of the university of Hirschwan No. 100 in Guangzhou City, guangdong, named Klebsiella sp.HN02 with a deposition number of GDMCC No:62011, the date of preservation is 2021, 11 and 4.
Furthermore, the 16S rRNA sequence of the Klebsiella is shown as SEQ ID NO. 3.
Further, the benzene series includes paraxylene.
Further, the benzene series includes gaseous para-xylene.
Further, the biosurfactant comprises a glycolipid biosurfactant.
A second object of the present invention is to propose a specific method for screening and identifying bacteria of the genus Klebsiella having a self-generating surfactant while improving the degradation capacity of gaseous organic pollutants.
Further, the screening method comprises the following steps:
injecting the sludge of the aerobic tank of the papermaking sewage treatment plant into a container filled with inorganic salt culture aqueous solution for culture, adding paraxylene, and sealing the container; measuring the concentration of paraxylene in the container in the culture process, and obtaining the bacterial liquid subjected to preliminary screening when the concentration of paraxylene is reduced to 0; carrying out streak separation and culture on the bacterial liquid subjected to preliminary screening on a blood agar plate, then picking a bacterial colony from the blood agar plate, and inoculating the bacterial colony on a methylene blue plate, wherein the bacterial strain capable of generating a color change reaction is regarded as bacteria which have the capability of degrading paraxylene and can self-produce a surfactant; and finally, repeating the above process on the strains obtained by screening to obtain the bacteria which have the capability of degrading the paraxylene and can self-produce the surfactant.
Wherein the sludge in the aerobic tank is obtained by mixing the sludge discharged from the aerobic tank with papermaking black liquor and culturing for at least 3 days.
In the invention, the papermaking black liquor refers to wastewater generated in an alkaline (caustic soda and sulfate) pulping process of a paper mill. The wastewater (black liquor) produced by the kraft pulping process is used in the examples of the present invention.
Further, the addition amount of the papermaking black liquor can be 1-3 times of the volume of the sludge, the pH can be controlled between 8-9, and the culture time can be controlled to be about 3-5 d.
Further, the aqueous solution for inorganic salt culture per 1000mL contains: 1.2g K 2 HPO 4 ·3H 2 O，1.2g KH 2 PO 4 ，0.2g MgSO 4 ·7H 2 O，0.4g NH 4 Cl，0.01g FeSO 4 ·7H 2 O and 1mL of trace element aqueous solution. Wherein, each 1000mL of microelement aqueous solution contains: 0.2g CaCl 2 ，0.2g MnSO 4 ·4H 2 O，0.1g CuSO 4 ·5H 2 O，0.2g ZnSO 4 ·7H 2 O，0.09g CoCl 2 ·6H 2 O，0.12g Na 2 MoO 4 ·2H 2 O and 0.006g H 3 BO 3 。
The invention also provides application of the self-generating surfactant and the klebsiella of the degradable benzene series in environmental remediation.
Further, the environment includes the atmosphere, a body of water, or soil.
The invention has the following effects:
1. the klebsiella HN02 strain is screened from the aerobic tank activated sludge of a sewage treatment plant in Hainan province for the first time, has the capability of degrading paraxylene and can self-produce a biological surfactant.
2. The klebsiella HN02 disclosed by the invention has the capability of degrading the paraxylene, and the emulsifying effect of the biosurfactant in a product obtained by culturing the klebsiella HN02 by taking the paraxylene as a carbon source is better.
3. The klebsiella HN02 disclosed by the invention has 100% degradation to paraxylene with the concentration of 5.2-26.0 mg/L, and has higher degradation efficiency to high-concentration (15.5-26.0 mg/L) paraxylene dissolution.
4. The degradation efficiency of the klebsiella HN02 of the invention to the substrate concentration of 26mg/L in the range of 20-40 ℃ is 100%.
5. The degradation efficiency of the klebsiella HN02 of the invention on the substrate concentration of 26mg/L in the pH range of 3-9 reaches 100%.
6. The papermaking wastewater is treated to generate a large amount of activated sludge, and the activated sludge contains zoogloea formed by various microorganisms, organic matters and inorganic matters. No report on screening and separating strains capable of generating a surfactant and degrading benzene series from an aerobic tank of a papermaking sewage treatment plant is found at present. Therefore, the proposal of the proposal can fill the technical blank in the field.
7. The invention provides a green and environment-friendly candidate scheme for the treatment of paraxylene in the environment.
Drawings
FIG. 1 is a diagram showing the morphology of Klebsiella sp HN02 of the present invention under an electron microscope.
FIG. 2 is a graph showing the effect of emulsification of the surfactant produced by Klebsiella HN02 of the present invention.
FIG. 3 is a species identification map of Klebsiella HN02 self-produced surfactant of the present invention.
Detailed Description
The invention will be further described with reference to specific examples, wherein the screening methods used in the present invention are specific methods, and bacteria having both paraxylene degrading and self-generating surfactant capabilities can be screened only by the methods described in the present invention, and blood agar plates and methylene blue plates are essential.
EXAMPLE 1 isolation screening of Klebsiella HN02
The inorganic salt culture aqueous solution used: every 1000mL of inorganic salt culture water solution contains 1.2. 1.2g K 2 HPO 4 ·3H 2 O，1.2g KH 2 PO 4 ，0.2g MgSO 4 ·7H 2 O，0.4g NH 4 Cl，0.01g FeSO 4 ·7H 2 O and 1mL of trace element aqueous solution. Wherein each 1000mL of trace element water solution contains 0.2g of CaCl 2 ，0.2g MnSO 4 ·4H 2 O,0.1g CuSO 4 ·5H 2 O，0.2g ZnSO 4 ·7H 2 O，0.09g CoCl 2 ·6H 2 O，0.12g Na 2 MoO 4 ·2H 2 O and 0.006g H 3 BO 3 。
The klebsiella HN02 of the embodiment is used for separating and screening the aerobic tank activated sludge from a papermaking sewage treatment plant in Hainan province. Placing sludge discharged from an aerobic tank of a papermaking sewage treatment plant into a 500mL bottle, adding papermaking black liquor with the volume being 2 times that of the sludge, adjusting the pH value to 8.3, sealing the bottle, performing shake culture at the temperature of 37 ℃ for 3 days at 200rpm, and leaching the black liquor to obtain sludge in the aerobic tank; 10mL of the aerobic tank sludge was poured into a 500mL headspace bottle containing 90mL of an aqueous inorganic salt culture solution, and cultured at 200 rpm. Filter sterilized paraxylene (1 μl) was added with a 10 μl syringe and the bottle was sealed with a stopper. The culture was incubated at 37℃for several days, and the concentration of paraxylene in the headspace bottle was measured by gas chromatography every 24 hours until the concentration of paraxylene was 0. When the concentration of the paraxylene is reduced to 0, the paraxylene degrading bacteria can be considered as being screened out preliminarily. The screening of the surfactant is carried out according to the characteristic that bacteria capable of producing the surfactant have hemolysis commonly, and the screening is further carried out by matching with the color-changing effect indicated by a methylene blue flat plate. The method comprises the steps of marking and separating bacterial liquid which is screened preliminarily and can degrade paraxylene on a blood agar plate, culturing in a constant temperature incubator at 37 ℃, picking bacterial colonies from the blood plate, inoculating the bacterial liquid on a methylene blue plate, and enabling the bacterial liquid capable of producing color change reaction to be preliminarily regarded as bacteria with paraxylene degradation capability and capable of producing surfactant. And finally, repeating the above process for 3 times on the obtained strain to obtain the bacteria which have the capability of degrading the paraxylene and can self-produce the surfactant.
Determination of degradation Rate: the degradation rate was measured by gas chromatography with timed sampling during the biodegradation of paraxylene. Degradation rate= (initial concentration-final concentration)/initial concentration.
Gas chromatography to determine the concentration of para-xylene: the sample inlet temperature of the gas chromatograph was set at 180℃and the column temperature of the chromatograph column was set at 90℃and the detector temperature was set at 220℃using a Fuli 9790ii gas chromatograph-hydrogen flame (GC-FID). The volume of the gas phase in the headspace bottle was drawn up to 200 μl using a 500 μl headspace gas-tight needle for sampling determination. The peak area at the initial time was recorded as the initial concentration, and the peak area at the final time was the final concentration.
The colony obtained by purification is identified, and the identification result is as follows:
(1) Morphological characteristics of the cells:
a. adopting a conventional bacterial physiological and biochemical identification method and an electron microscope for observation, and staining the screened cells of the klebsiella into gram negative; the morphology was short rod-like when observed under an electron microscope, and the cell size was 1-3. Mu.m, as shown in FIG. 1.
b. The morphological characteristics of the colony, namely, the colony is round, convex, neat in edge and smooth in surface after being cultured on an NA (nutrient agar) plate for 2 days.
c. The main physiological and biochemical characteristics of klebsiella are shown in table 1:
TABLE 1 physiological and biochemical characteristics of Klebsiella HN02
Note that: +: positive; -: negative of
The above results indicate that the bacteria selected in the present invention are very similar to the physiological and biochemical characteristics of klebsiella.
(2) Bacterial genomic DNA was extracted using bacterial 16S rDNA universal primers:
an upstream primer: V4-515F (5'-GTGCCAGCAGCCGCGGTAA-3'), SEQ ID NO:1
A downstream primer: V4-806R (5'-GGACTACCAGGGTATCTAA-3'), SEQ ID NO:2
Amplifying all the genes of the 16S rDNA, and sequencing the genes to obtain the sequence shown in SEQ ID NO: 3.
Setting SEQ ID NO:3, and comparing the 16SrRNA gene sequence with the length of 1394bp shown in the step 3 with the registered gene sequence in Genbank, the homology of the strain with Klebsiella pneumoniae strain MGH83 is found to be 99.93 percent.
In view of the above physiological and biochemical characteristics and the 16S rRNA gene sequence results, the strain selected by the present invention should belong to a new variety of Klebsiella, and is named as Klebsiella (Klebsiella) HN02.
The strain is named as Klebsiella (Klebsiella) HN02, is currently deposited in the Guangdong institute of microbiology (GDMCC), and has a deposition number of 62011, a deposition address of Guangzhou, first, china, no. 100, no. 59, building 5, and a deposition date of 2021, 11, 4 days.
Example 2
This example is the use of Klebsiella (Klebsiella) HN02 in environmental remediation to degrade paraxylene in the environment. Where the environment includes the atmosphere, a body of water, or soil.
The p-xylene degradation ability of Klebsiella (Klebsiella) HN02 obtained by screening according to the present invention was tested:
according to the degradation experiment requirement, preparing an inorganic salt culture medium: 100mL of an inorganic salt solution (1.2. 1.2g K per 1000mL of an inorganic salt culture aqueous solution) was added to a 500mL headspace bottle 2 HPO 4 ·3H 2 O，1.2g KH 2 PO 4 ，0.2g MgSO 4 ·7H 2 O，0.4g NH 4 Cl，0.01g FeSO 4 ·7H 2 O and 1mL of trace element aqueous solution. Wherein, each 1000mL of microelement aqueous solution contains: 0.2g CaCl 2 ，0.2g MnSO 4 ·4H 2 O，0.1g CuSO 4 ·5H 2 O，0.2g ZnSO 4 ·7H 2 O，0.09g CoCl 2 ·6H 2 O，0.12g Na 2 MoO 4 ·2H 2 O and 0.006g H 3 BO 3 ). Autoclaving at 121℃for 15min. Firstly, the Klebsiella HN02 with paraxylene degrading ability obtained by screening is activated for 48 hours in a shaking table with 37 ℃ and 200rprn in LB culture medium (yeast extract 5.0g/L, peptone 10.0g/L, naCl 5.0.0 g/L), bacterial liquid is centrifuged, bacterial cells are collected, and washed three times with inorganic salt culture aqueous solution, and resuspended with 100mL inorganic salt solution, 100mL bacterial liquid (4×10) 8 CFU/mL) was injected into headspace bottles with different contents of para-xylene added in advance using a 100mL syringe, and the para-xylene concentrations in the headspace bottles were respectively: 5.2 10.3, 15.5, 21.0, 26.0mg/L. Wherein the pH value of the inorganic salt water solution is 7, the reaction is carried out by a shaking table at 37 ℃ and 200rpm, the sample is taken at regular time, and the degradation rate is measured by gas chromatography. Degradation Rate determination As in example 1, knotThe results are shown in Table 2.
TABLE 2
As can be seen from Table 2, the Klebsiella (Klebsiella) HN02 obtained by screening of the invention can realize 100% degradation of paraxylene under the condition that the degradation capacity of paraxylene is between 5.2 and 26.0mg/L. Further, it is found that the degradation efficiency of Klebsiella (Klebsiella) HN02 against high concentration (15.5 to 26.0 mg/L) of paraxylene is higher in terms of degradation concentration per unit time.
Further experiments prove that the degradation efficiency of the klebsiella HN02 on the substrate concentration of 26mg/L reaches 100% in the range of 20-40 ℃. The degradation efficiency of the substrate concentration of 26mg/L in the pH range of 3-9 reaches 100%. Degradation time is within 120 h.
Example 3
This example is an demonstration of Klebsiella (Klebsiella) HN02 in terms of its ability to produce biosurfactants.
The biological surfactant ability produced by Klebsiella (Klebsiella) HN02 obtained by screening in the invention is tested:
klebsiella was cultured at a substrate concentration of 21mg/L, and the culture conditions were as described in example 2. And (5) taking the bacterial liquid supernatant after the paraxylene is completely degraded, and measuring the emulsifying capacity of the bacterial liquid supernatant. The specific method comprises the steps of adding 3mL of peanut oil into a test tube, adding the cell-free supernatant obtained after the same amount of centrifugal bacterial liquid into the same test tube, vigorously shaking the test tube for 2min, standing for 24h, and observing the height of an emulsion layer after 24 h. Emulsification index= (emulsion layer height/total height of liquid in test tube) ×100%.
According to the measurement, the emulsification index of the biosurfactant produced by the Klebsiella HN02 obtained by screening in the invention is 37.50%, which shows that the emulsification effect of the surfactant produced by the Klebsiella is obvious, and the biosurfactant is proved to be produced as shown in figure 2.
Comparative example 1
For comparative analysis of the effect of paraxylene on the surfactant production capacity of Klebsiella (Klebsiella) HN02. In this example, klebsiella (Klebsiella) HN02 was cultured using potato starch instead of paraxylene as a carbon source, and the emulsification index was measured.
The culture conditions were the same as in example 3. After the culture, the bacterial liquid supernatant was taken and subjected to emulsification capacity measurement. The specific method comprises the steps of adding 3mL of peanut oil into a test tube, adding the cell-free supernatant obtained after the same amount of centrifugal bacterial liquid into the same test tube, vigorously shaking the test tube for 2min, standing for 24h, and observing the height of an emulsion layer after 24 h. Emulsification index= (emulsion layer height/total height of liquid in test tube) ×100%.
The resulting biosurfactant was determined to have an emulsification index of 28.59%. The results of comparative example 3 and comparative example 1 show that klebsiella HN02 can utilize paraxylene to increase the capacity of self-produced surfactant, and the emulsification effect of the produced surfactant is better.
Comparative example 2
The invention also compares the application effect of the strain obtained by adopting different screening methods.
Screening method (1): i.e. the screening method described in example 1.
Screening method (2): placing sludge discharged from an aerobic tank of a papermaking sewage treatment plant into a 500mL bottle, adding inorganic salt culture aqueous solution with the volume of 2 times into the sludge, adjusting the pH value to 8.3, sealing the bottle, performing shake culture at the temperature of 37 ℃ for 3d at 200rpm, and leaching black liquor to obtain sludge in the aerobic tank; 10mL of the aerobic tank sludge was poured into a 500mL headspace bottle containing 90mL of an aqueous inorganic salt culture solution, and cultured at 200 rpm. Filter sterilized paraxylene (1 μl) was added with a 10 μl syringe and the bottle was sealed with a stopper. The culture was incubated at 37℃for several days, and the concentration of paraxylene in the headspace bottle was measured by gas chromatography every 24 hours until the concentration of paraxylene was 0. And (3) carrying out streak separation on the preliminarily screened bacterial liquid capable of degrading paraxylene on a blood agar plate, culturing in a constant temperature incubator at 37 ℃, picking bacterial colonies from the blood plate, inoculating the bacterial colonies on a methylene blue plate, and selecting a strain capable of producing a color change reaction. Repeating the above process for the obtained strain to obtain purified bacteria.
Strains obtained by the different screening methods were cultured by the culture method of example 3, and the emulsifying capacity was measured.
The results showed that the strain obtained in the method (2) produced biosurfactant with an emulsification index of 12.34%, indicating that the strain obtained in the method (1) had a higher surfactant-producing ability.
Example 4
This example is a class-dependent identification of the biosurfactant produced by Klebsiella (Klebsiella) HN02.
The biological surfactant capability generated by Klebsiella (Klebsiella) HN02 obtained by screening of the invention is subjected to nuclear magnetic resonance hydrogen spectrum 1 HNMR) identification:
100mL of the bacterial liquid (4X 10) described in example 2 was used 8 CFU/mL) and 1mL of liquid paraxylene, shaking culture at 37 deg.c and 200rpm for 3 days, centrifuging the bacterial liquid to eliminate bacterial cells, extracting with ethyl acetate for 10 min repeatedly, separating to obtain organic phase, evaporating the organic phase in a rotary evaporator at 50 deg.c to obtain brown solid, dissolving in dimethyl sulfoxide (DMSO), and performing nuclear magnetic resonance hydrogen spectrum 1 HNMR) identification.
According to the measurement, the biological surfactant generated by Klebsiella (Klebsiella) HN02 obtained by screening belongs to glycolipid biological surfactants, key indication peaks are glycosidic bonds and related aliphatic groups existing in the presence of sugar molecules, and the related identification results are shown in figure 3.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (7)
1. A Klebsiella strain capable of generating a surfactant and a degradable benzene series is characterized in that the Klebsiella strain is deposited in the Guangdong institute of microbiology and is named as a strain collectionKlebsiella sp.HN02, accession No. GDMCC No:62011, the date of preservation is 2021, 11 and 4.
2. The self-producing surfactant and the klebsiella of a degradable benzene series according to claim 1, wherein the 16S rRNA sequence of the klebsiella is shown in SEQ ID No. 3.
3. The self-generating surfactant of claim 1 and a degradable benzene-based bacterium, wherein the benzene-based bacterium comprises paraxylene.
4. The self-generating surfactant of claim 1 and a degradable benzene-based bacterium, wherein the benzene-based bacterium comprises gaseous para-xylene.
5. The self-generating surfactant and the degradable benzene-based klebsiella of claim 1, wherein the biosurfactant comprises a glycolipid biosurfactant.
6. The use of the self-generating surfactant of any one of claims 1 to 5 and a klebsiella which is a degradable benzene series, wherein the benzene series is paraxylene, in environmental remediation.
7. The use of claim 6, wherein the environment comprises the atmosphere, a body of water, or soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111628837.7A CN114317346B (en) | 2021-12-28 | 2021-12-28 | Klebsiella strain capable of generating object surfactant and degradable benzene series and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111628837.7A CN114317346B (en) | 2021-12-28 | 2021-12-28 | Klebsiella strain capable of generating object surfactant and degradable benzene series and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114317346A CN114317346A (en) | 2022-04-12 |
| CN114317346B true CN114317346B (en) | 2023-09-08 |
Family
ID=81014109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111628837.7A Active CN114317346B (en) | 2021-12-28 | 2021-12-28 | Klebsiella strain capable of generating object surfactant and degradable benzene series and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114317346B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055282A (en) * | 2018-09-27 | 2018-12-21 | 陕西科技大学 | One Klebsiella pneumoniae new strains and its separation method and application |
-
2021
- 2021-12-28 CN CN202111628837.7A patent/CN114317346B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055282A (en) * | 2018-09-27 | 2018-12-21 | 陕西科技大学 | One Klebsiella pneumoniae new strains and its separation method and application |
Non-Patent Citations (1)
| Title |
|---|
| AHLs对不同菌株产生物胺的影响及菌株生物被膜特性分析;陆静 等;现代食品科技;第30卷(第10期);第88-95页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114317346A (en) | 2022-04-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110257310B (en) | A kind of vinyon efficient degrading bacteria and its separating screening method and application | |
| CN108753662B (en) | Low-temperature dimethyl phthalate high-efficiency degrading bacterium and fermentation method thereof | |
| CN106434470B (en) | A kind of polycyclic aromatic hydrocarbon-degrading bacteria and its application | |
| CN107904188A (en) | One plant of achromobacter with monomethyl amine degradation capability and its application | |
| US20200308535A1 (en) | Pseudomonas aeruginosa with monomethylamine degradability and application thereof | |
| CN114045239B (en) | Paracoccus ubiquitously YBH-7 with dimethylacetamide degradation capability and application thereof | |
| US6383797B1 (en) | Bacterial consortium EBC1000 and a method using the bacterial consortium EBC1000 for remedying biologically recalcitrant toxic chemicals contained in industrial wastewater, waste materials and soils | |
| CN113462622B (en) | Pseudomonas for efficiently degrading various aromatic pollutants and application thereof | |
| CN114107092B (en) | Endophyte Gordonia L191 for degrading phthalate and application thereof | |
| CN109706096A (en) | One plant of brevibacterium frigoritolerans and its application with denitrogenation and efficient flocculating ability | |
| CN114317346B (en) | Klebsiella strain capable of generating object surfactant and degradable benzene series and application thereof | |
| CN102766580B (en) | Yeast strain for producing biosurfactant and application thereof | |
| CN109337825B (en) | Paecilomyces beijing strain LYZ7 and application thereof | |
| CN115386520B (en) | Rhodococcus pyridine-philic RL-GZ01 strain and application thereof | |
| CN110229766A (en) | Aoxidize microbacterium and its application in degradable organic pollutant | |
| CN113980851B (en) | Paracoccus YBH-X with dimethylacetamide degradation capability and application thereof | |
| CN114045238B (en) | Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application thereof | |
| CN101624576B (en) | Mycobacterium cosmeticum with capacity of degrading benzene compound and application thereof | |
| CN111534458B (en) | Achromobacter TBC-1 and application thereof in degradation of 1,3,6,8-tetrabromocarbazole | |
| CN109055254B (en) | Microbacterium with monomethylamine degradation capability and application thereof | |
| CN113583896A (en) | Enterobacter huoshanense and application thereof | |
| CN113249276A (en) | Bacillus cereus and application thereof | |
| CN114250185B (en) | Enterobacter capable of degrading dimethylbenzene and generating surfactant automatically and application thereof | |
| CN105062911B (en) | One kind is for biodegradable binary composite flora construction method in waste water | |
| CN114395502B (en) | Nitro-reduction pseudomonas WX4-1 for efficiently degrading tetramethylene and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |