CN114317232A - Concentric liquid path mechanism applied to PCR instrument - Google Patents
Concentric liquid path mechanism applied to PCR instrument Download PDFInfo
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- CN114317232A CN114317232A CN202210021076.7A CN202210021076A CN114317232A CN 114317232 A CN114317232 A CN 114317232A CN 202210021076 A CN202210021076 A CN 202210021076A CN 114317232 A CN114317232 A CN 114317232A
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Abstract
The invention discloses a concentric liquid path mechanism applied to a PCR instrument, which comprises a main body, a flow guide mechanism, a sealing mechanism and a detection mechanism, wherein the flow guide mechanism is arranged on the main body, and a separation cavity is formed between the flow guide mechanism and the main body; the diversion mechanism is provided with a liquid drop outlet and a drainage groove, and the drainage groove is communicated with the separation cavity; the sealing mechanism is arranged between the flow guide mechanism and the main body; the detection mechanism comprises a detection tube and a mounting seat, the detection tube is fixedly mounted on the mounting seat in a sealing mode, the mounting seat is fixedly connected with the main body, and the detection tube is located at the liquid drop outlet and coaxial with the liquid drop outlet. The scheme of the invention can realize the full package of the liquid drops in the detection passage, so that the liquid drops are stressed more evenly, and the straightness in the detection passage is higher.
Description
Technical Field
The invention relates to a biomedical detection device, in particular to a concentric liquid path mechanism applied to a PCR instrument.
Background
The digital PCR technology is an absolute quantitative technology of nucleic acid molecules developed in the late 90 s of the 20 th century, and distributes a fluorescent quantitative PCR reaction system into a large number of micro reactors, wherein each micro reactor contains or does not contain one or more copies of target nucleic acid molecules, and the single molecular template PCR amplification is carried out. And calculating the copy number of the target gene in the original sample by the number of positive reaction units and a statistical method after the amplification is finished.
The micro-drop digital PCR instrument is to make sample containing nucleic acid molecule into several tens of thousands of drops and make them in another continuous phase insoluble in the drops to make emulsion containing "water-in-oil" or "oil-in-water" drops.
Drop-based digital assays typically rely on statistical analysis of the total number of drops from an emulsion to obtain results. The measurement is more accurate when a larger number of droplets are detected. The droplets sequentially pass through the detection channel in a linear arrangement, so that the loss of the number of detected droplets due to the overlapping of a plurality of droplets can be reduced. For example, deflection of light by a droplet may produce a waveform in the deflection signal detected from the detection region, where the width of the waveform corresponds to the size of the droplet. When a plurality of droplets overlap, the waveform width of the detection signal at the detection point is not consistent with the normal waveform and is screened out. The uniformity with which the droplets are sequentially passed through the detection channel at certain intervals determines the confidence level of the collected light deflection signal.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a concentric liquid path mechanism applied to a PCR instrument, so that the liquid drops are completely wrapped in a detection path, the stress of the detection path is more balanced, and the straightness in the detection path is higher.
In order to achieve the above object, the present invention adopts a technical solution of a concentric fluid path mechanism for a PCR instrument, comprising:
a main body;
the flow guide mechanism is arranged on the main body, and a separation cavity is formed between the flow guide mechanism and the main body; the diversion mechanism is provided with a liquid drop outlet and a drainage groove, and the drainage groove is communicated with the separation cavity;
the sealing mechanism is arranged between the flow guide mechanism and the main body; and
the detection mechanism comprises a detection tube and a mounting seat, the detection tube is fixedly mounted on the mounting seat in a sealing mode, the mounting seat is fixedly connected with the main body, and the detection tube is located at the liquid drop outlet and coaxial with the liquid drop outlet.
In an embodiment of the invention, the main body is provided with an inner groove, the flow guide mechanism is provided with a lower flange matched with the inner groove, the lower flange is provided with an external thread, and the flow guide mechanism is in threaded connection with the main body.
In an embodiment of the present invention, the drainage groove is a plurality of notches formed at the external thread.
In an embodiment of the invention, the sealing mechanism is disposed around the lower flange of the flow guide mechanism.
In an embodiment of the present invention, the flow guiding mechanism is provided with a plurality of flow spacing mechanisms, the flow spacing mechanisms are communicated with the drainage grooves, and the plurality of flow spacing mechanisms are uniformly arranged.
In an embodiment of the present invention, the spacer flow mechanism includes a mounting head and a spacer tube, the mounting head is fixedly connected to the spacer tube in a sealing manner, the mounting head is fixedly mounted on the flow guide mechanism, and the spacer tube extends to the drainage groove and is communicated with the drainage groove.
In an embodiment of the present invention, the flow guiding mechanism is provided with an emulsion tube and a connector, the emulsion tube and the connector are fixedly connected in a sealing manner, the connector is fixedly connected with the flow guiding mechanism, and the emulsion tube is communicated with the droplet outlet.
In an embodiment of the present invention, the emulsion tube is coaxial with the detection tube.
In an embodiment of the invention, the separation chamber is a conical cavity.
The technical scheme has the following beneficial effects:
according to the invention, through the concentric design of the liquid path, emulsion enters the separation cavity through a liquid drop outlet on the diversion mechanism, and meanwhile, interval flow enters the drainage groove through the diversion mechanism, the drainage groove drains the interval flow to the separation cavity, and the separation cavity gradually shrinks the interval flow to be the same as the diameter of the detection tube; the separation flow and the droplet flow enter the detection tube at the tail end of the separation cavity in a laminar flow with the cross section being a concentric ring, and the droplets are positioned in the core flow of the coaxial fluid under the action of the separation flow, so as to sequentially enter the detection channel in a linear arrangement mode.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is an axial cross-sectional view of a flow directing mechanism according to an embodiment of the present invention;
FIG. 3 is a vertical cross-sectional view of a deflector mechanism according to an embodiment of the invention;
FIG. 4 is a schematic diagram of a concentric fluid path mechanism in accordance with an embodiment of the present invention;
FIG. 5 is a schematic diagram of the formation of a linear arrangement of droplets according to an embodiment of the present invention.
Detailed Description
The invention will now be further described with reference to the following examples and figures 1 to 5.
A concentric liquid path mechanism applied to a PCR instrument is shown in figure 1 and comprises a main body 1, a flow guide mechanism 2, a sealing element 3, a first mounting head 4a, a second mounting head 4b, a mounting seat 5, a connector 6, a capillary tube 7, an emulsion tube 8, a first sheath tube 9a and a second sheath tube 9 b. The capillary 7 serves as a detection line, the emulsion tube 8 is used to provide liquid droplets, and the first sheath tube 9a and the second sheath tube 9b are used to provide a spacer flow. The main body 1 is used for being connected with the detection channel, the flow guide mechanism 2 is used for installing an emulsion inlet pipeline and a spacing fluid inlet pipeline, and the flow guide mechanism is matched with the main body through threads. The flow guide mechanism 2 and the main body structure form a separation cavity, and the axial section of the separation cavity is a circular ring concentric with the emulsion inlet. The flow guide mechanism 2 is provided with a liquid drop outlet and a drainage groove, and the drainage groove is communicated with the separation cavity; the detection mechanism comprises a detection tube and a mounting seat, the detection tube is fixedly mounted on the mounting seat in a sealing mode, the mounting seat is fixedly connected with the main body, and the detection tube is located at the liquid drop outlet and coaxial with the liquid drop outlet.
The novel water-saving device is characterized in that an inner groove is formed in the main body 1, the flow guide mechanism is provided with a lower flange matched with the inner groove, an external thread is arranged on the lower flange, and the flow guide mechanism is in threaded connection with the main body. And drainage grooves are formed in two sides of the thread and used for draining the spacer fluid to the separation cavity. As shown in fig. 2, which is an axial sectional view of the air guide mechanism 2. The thread of the diversion mechanism is provided with a notch which is a first diversion groove 10a and a second diversion groove 10b respectively. One end of the first drainage groove 10a communicates with the sheath fluid inlet 11a, and the second drainage groove 10b communicates with the sheath fluid inlet 11 b. The sealing element 3 is used for sealing the separation cavity, and the sealing element 3 is arranged around the lower flange of the flow guide mechanism.
In this example, the main body 1 is connected to the diversion mechanism 2 by a screw thread, the capillary tube 7 is mounted at the bottom of the main body 1 by the mounting seat 5, and the other three lotion tubes 8, the first sheath tube 9a and the second sheath tube 9b are respectively fixed on the diversion mechanism by the connecting head 6, the first mounting head 4a and the second mounting head 4 b. The emulsion tube 8, the first sheath liquid tube 9a and the second sheath liquid tube 9b are input ends, the capillary tube 7 is an output end, and the structure enables the multi-channel system to achieve one-step disassembly and assembly of the output end and the input end. The guide mechanism 2 is arranged on the main body 1, and the height of a flange at the bottom of the guide mechanism 2 relative to the limiting surface is lower than the depth of a groove at the corresponding position of the main body 1, so that a profiling cavity of the flange is formed. In addition, the diversion mechanism 2 forms three input ends which are all installed on one piece, and the separation input module only needs to be screwed off by one thread.
Fig. 4 is a schematic diagram of a concentric fluid path mechanism. Emulsion containing liquid drops enters a separation cavity through a flow channel 13, sheath liquid enters the separation cavity 14 through the flow channel 12a and the flow channel 12b under the action of a drainage groove 10a and a drainage groove 10b of a drainage mechanism respectively, the separation cavity is a conical cavity formed after the drainage mechanism and a main body are installed, so that the sheath liquid on two sides is combined into a beam by two beams of fluid, the beam is a circular ring which is coaxial with the flow channel 13 when viewed from an axial interface, and the width of the circular ring is reduced along the flowing direction of the sheath liquid, and the diameter of the outlet of the separation cavity is slightly larger than the aperture of a detection channel.
As shown in fig. 5, the principle diagram of the formation of the linear arrangement of the droplets at the outlet of the separation chamber is shown. Under the action of the focusing flow channel of the separation cavity 14, the sheath liquid enables the liquid drops which are freely and disorderly arranged in the flow channel 13 to sequentially enter a detection channel which is slightly larger than the liquid drops, and because the sheath liquid forms a radial continuous coaxial fluid on the periphery of the emulsion under the action of the concentric annular flow channel, the straightness of the liquid drops is higher.
The above-described embodiments are intended to illustrate rather than to limit the invention, and any changes and alterations made without inventive step within the spirit and scope of the claims are intended to fall within the scope of the invention.
Claims (9)
1. A concentric liquid way mechanism applied to a PCR instrument is characterized by comprising:
a main body;
the flow guide mechanism is arranged on the main body, and a separation cavity is formed between the flow guide mechanism and the main body; the diversion mechanism is provided with a liquid drop outlet and a drainage groove, and the drainage groove is communicated with the separation cavity;
the sealing mechanism is arranged between the flow guide mechanism and the main body; and
the detection mechanism comprises a detection tube and a mounting seat, the detection tube is fixedly mounted on the mounting seat in a sealing mode, the mounting seat is fixedly connected with the main body, and the detection tube is located at the liquid drop outlet and coaxial with the liquid drop outlet.
2. The concentric fluid path mechanism as claimed in claim 1, wherein the main body has an inner groove, the fluid guiding mechanism has a lower flange engaged with the inner groove, the lower flange has an outer thread, and the fluid guiding mechanism is connected to the main body by a thread.
3. The concentric fluid path mechanism of claim 2, wherein the drainage groove is a plurality of notches cut in the external thread.
4. The concentric fluid circuit mechanism of claim 2 wherein the sealing mechanism is disposed around a lower flange of the flow directing mechanism.
5. The concentric fluid path mechanism as claimed in claim 1, wherein the flow guiding mechanism is provided with a plurality of flow spacing mechanisms, the flow spacing mechanisms are communicated with the drainage grooves, and the plurality of flow spacing mechanisms are uniformly arranged.
6. The concentric fluid path mechanism of claim 5 wherein the spacer means comprises a mounting head and a spacer tube, the mounting head is sealingly and fixedly connected to the spacer tube, the mounting head is fixedly mounted to the flow directing means, and the spacer tube extends to and communicates with the flow directing channel.
7. The concentric liquid path mechanism as claimed in claim 1, wherein the guiding mechanism is provided with an emulsion tube and a connector, the emulsion tube is fixedly connected with the connector in a sealing manner, the connector is fixedly connected with the guiding mechanism, and the emulsion tube is communicated with the liquid drop outlet.
8. The concentric fluid path mechanism of claim 7 wherein the emulsion tube is coaxial with the detection tube.
9. The concentric fluid circuit mechanism of any one of claims 1 to 8 wherein the separation chamber is a conical cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210021076.7A CN114317232A (en) | 2022-01-10 | 2022-01-10 | Concentric liquid path mechanism applied to PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210021076.7A CN114317232A (en) | 2022-01-10 | 2022-01-10 | Concentric liquid path mechanism applied to PCR instrument |
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| CN114317232A true CN114317232A (en) | 2022-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210021076.7A Pending CN114317232A (en) | 2022-01-10 | 2022-01-10 | Concentric liquid path mechanism applied to PCR instrument |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030137666A1 (en) * | 2001-12-18 | 2003-07-24 | Johnson Paul E. | Apparatus and methods for high throughput analysis of samples in a translucent flowing liquid |
| CN1876801A (en) * | 2006-04-29 | 2006-12-13 | 北京索通医疗技术有限公司 | Cell detection transducer |
| US20180334670A1 (en) * | 2017-05-18 | 2018-11-22 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US20190002956A1 (en) * | 2017-06-28 | 2019-01-03 | Bio-Rad Laboratories, Inc. | System and method for droplet detection |
| US20200086312A1 (en) * | 2008-09-23 | 2020-03-19 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
| CN113358573A (en) * | 2021-06-09 | 2021-09-07 | 安徽皖仪科技股份有限公司 | Optical detection flow cell |
-
2022
- 2022-01-10 CN CN202210021076.7A patent/CN114317232A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030137666A1 (en) * | 2001-12-18 | 2003-07-24 | Johnson Paul E. | Apparatus and methods for high throughput analysis of samples in a translucent flowing liquid |
| CN1876801A (en) * | 2006-04-29 | 2006-12-13 | 北京索通医疗技术有限公司 | Cell detection transducer |
| US20200086312A1 (en) * | 2008-09-23 | 2020-03-19 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
| US20180334670A1 (en) * | 2017-05-18 | 2018-11-22 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US20190002956A1 (en) * | 2017-06-28 | 2019-01-03 | Bio-Rad Laboratories, Inc. | System and method for droplet detection |
| CN113358573A (en) * | 2021-06-09 | 2021-09-07 | 安徽皖仪科技股份有限公司 | Optical detection flow cell |
Non-Patent Citations (1)
| Title |
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| 于虎;祝连庆;郭阳宽;孟晓辰;: "流式细胞仪流动室流场特性仿真" * |
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