CN114316224A - Preparation method and application of artificial purulent melanin nano material - Google Patents
Preparation method and application of artificial purulent melanin nano material Download PDFInfo
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Abstract
The invention discloses a preparation method and application of an artificial purplish melanin nano material, wherein the method comprises the following steps: preparing a sodium periodate solution and a gentisic acid solution, dropwise adding the sodium periodate solution into the gentisic acid solution, reacting for 8-24 hours, and sequentially performing centrifugal treatment and deionized water washing on the reaction solution to obtain an artificial pustulan nano material; wherein the dosage of the sodium periodate and the dosage of the gentisic acid are respectively 6 parts by weight and 2-20 parts by weight. The invention adopts a chemical method to synthesize the artificial purplish melanin nano material for the first time, and compared with a biosynthesis method, the method is more efficient, adjustable and controllable, has good repeatability and is suitable for industrial production. The prepared artificial melanin nano material has similar physicochemical properties to natural melanin, including regular particle morphology, good oxidation resistance, good ultraviolet light absorption capacity, good free radical scavenging capacity and the like, and can be used as an oxidation resistance material, a light absorption material, a photothermal conversion material and a photothermal sterilization material.
Description
Technical Field
The invention belongs to the technical field of artificial melanin, and particularly relates to a preparation method and application of an artificial purulent melanin nano material.
Background
Melanin is widely found in plants, animals, bacteria and fungi in nature, and mainly comprises eumelanin, pheomelanin, neuromelanin, melanoidin and pustulan. The loss of melanin can have great negative effects on the daily life and physical health of human beings. However, there are many difficulties in obtaining melanin from nature and further applying it.
Starting from precursor micromolecules of biomass, by reasonably designing a macromolecular physical and chemical method, artificial biomacromolecules with similar structures, properties and functions to natural biomacromolecules can be constructed. For example, the artificial eumelanin material can be constructed by starting from dopamine precursor small molecules and carrying out automatic oxidation under alkaline conditions. The artificial eumelanin material is similar to natural melanin and has good biological activity, so the artificial eumelanin material has received extensive attention. At present, artificial eumelanin materials are produced in batches and are applied to a plurality of fields. In recent years, researchers have also synthesized artificial melanoidin materials from dihydroxynaphthalene precursor molecules, and the artificial melanoidin materials have attracted much attention in the fields of biological treatment, energy application, radiation protection and the like.
Purplish melanin is nitrogen-free and water-soluble melanin in nature, mainly derived from various fungi and various bacteria, especially gamma proteobacteria, and is a catabolite of tyrosine or phenylalanine. Tyrosine or phenylalanine forms precursor small molecule gentisic acid under the action of a series of biological enzymes, which has a characteristic hydroquinone structure and a phenylacetic acid structure, and is automatically oxidized in a microorganism to form benzoquinone acetic acid, and then form a pustulan polymer. The pustulan also has good biological activity, plays an important physiological role in organisms, has proved to have a protective effect on microorganisms, and plays a positive role mainly through the photoprotection effect brought by spectral absorption and the antioxidation brought by a phenolic hydroxyl structure.
However, no mature chemical method is available for synthesizing artificial purplish materials at present. This is because on the one hand, the purulent melanin is relatively low in content in nature and is difficult to extract; on the other hand, compared with the reaction activities of dopamine, dihydroxynaphthalene and the like, the structure of the precursor micromolecule of the pustulan is limited. At present, the synthesis method aiming at the purulent melanin is mainly started from a biological path and is cultured and separated in bacteria or fungi. For example, in chinese patent application publication No. CN 107794263a, example 2 discloses a method for culturing and isolating melanoidins in strains. However, the biological methods generally have the problems of long production period, unstable process, large product difference, low yield and the like.
Disclosure of Invention
The invention aims to overcome the defects of the existing biological method for synthesizing artificial purplish melanin, and provides a preparation method and application of an artificial purplish melanin nano material. The method is realized based on a chemical method, and is more efficient and controllable.
The invention adopts the following technical scheme:
the preparation method of the artificial purulent melanin nano material comprises the following steps:
preparing a sodium periodate solution and a gentisic acid solution, dropwise adding the sodium periodate solution into the gentisic acid solution, reacting for 8-24 hours, and sequentially performing centrifugal treatment and deionized water washing on the reaction solution to obtain an artificial pustulan nano material; wherein the dosage of the sodium periodate and the dosage of the gentisic acid are respectively 6 parts by weight and 2-20 parts by weight.
In the invention, sodium periodate is used as an oxidant, and the pergentic acid is used as a precursor molecule of the pustulan, and nano particles are formed by polymerization and assembly in a solution, so that the artificial pustulan nano material is obtained.
In some embodiments, the sodium periodate and the gentisic acid are used in an amount of 6 parts by weight and 7-15.5 parts by weight, respectively.
In some embodiments, the sodium periodate and the gentisic acid are used in an amount of 6 parts by weight and 7-11.5 parts by weight, respectively.
In some embodiments, the sodium periodate and the gentisic acid are used in an amount of 6 parts by weight and 7 parts by weight, respectively.
The particle size of the artificial purplish nanometer material can be regulated and controlled by regulating and controlling the dosage ratio of the sodium periodate to the gentisic acid. When the using amounts of the sodium periodate and the pergentic acid are respectively 6 parts by weight and 7 parts by weight, the artificial purplish nanometer material with the particle size distribution of 147-165 nm can be obtained; when the using amounts of the sodium periodate and the pergentic acid are respectively 6 parts by weight and 11.5 parts by weight, the artificial melanin nano material with the particle size distribution of 205-289 nm can be obtained; when the using amounts of the sodium periodate and the pergentic acid are respectively 6 parts by weight and 15.5 parts by weight, the artificial purometanine nano material with the particle size distribution of 219-313 nm can be obtained.
In some embodiments, the reaction time is 16 to 24 hours.
In some embodiments, the method for preparing the artificial purplish nanometer material comprises the following specific steps:
(1) taking an ethanol-containing water solution as a solvent, and preparing a 2-4 mg/mL high gentisic acid solution, wherein the weight of ethanol in the water solution accounts for 10% -40%; carrying out ultrasonic dispersion treatment on the high gentisic acid solution for 3-10 minutes, and then stirring at room temperature to obtain a transparent colorless high gentisic acid solution;
(2) deionized water is used as a solvent, and a sodium periodate aqueous solution with the concentration of 100-300 mg/mL is prepared;
(3) slowly adding sodium periodate aqueous solution into the gentisic acid solution, maintaining stirring at room temperature, and completing the reaction when stable brown-black turbid solution is obtained; in some embodiments, the reaction time is 8 to 24 hours;
(4) and (4) sequentially centrifuging and washing the reaction solution to obtain the artificial purplish melanin nano material.
In some embodiments, the method further comprises: the photo-thermal reagent indocyanine green is loaded on the artificial purplish melanin nano material, and the loading capacity is 10-30 mug/mg.
The specific steps of the loaded photo-thermal reagent indocyanine green are as follows:
mixing the artificial pustulan nano material aqueous solution and the indocyanine green aqueous solution, oscillating at constant speed for 20-30 h in a constant temperature shaking table at room temperature, and then sequentially centrifuging and washing.
The artificial melanin nano material prepared by the method has good in-vitro free radical scavenging capacity and cell level oxidation resistance, and can be used as an oxidation resistant material.
The artificial purplish nanometer material prepared by the method has good ultraviolet absorption capacity and can be used as a light absorption material.
The artificial purulent melanin nano material prepared by the method has excellent photo-thermal conversion capacity and can be used as a photo-thermal conversion material.
The artificial purulent melanin nano material prepared by the method has good photo-thermal sterilization capability and can be used as a photo-thermal sterilization material.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the method starts from the precursor micromolecule high gentisic acid, and synthesizes the artificial purplish melanin nano material by a chemical method for the first time.
(2) The prepared artificial melanin nano material has similar physicochemical properties with natural melanin, including regular particle morphology, good oxidation resistance, good ultraviolet light absorption capacity, good free radical scavenging capacity and the like.
(3) The artificial melanin nano material prepared by the invention has good in-vitro free radical scavenging capacity and cell level oxidation resistance, and can be used as an oxidation resistant material.
(4) The artificial purplish nanometer material prepared by the invention has good ultraviolet light absorption capacity and can be used as a light absorption material.
(5) The artificial melanin nano material prepared by the invention has excellent photo-thermal conversion performance and can be used as a photo-thermal conversion material.
(6) The activity of escherichia coli and staphylococcus aureus under near infrared light proves the good photo-thermal sterilization capability of the composite material, and the composite material can be used as a photo-thermal sterilization material.
(7) The artificial purulent melanin nano material prepared by the invention has lower cytotoxicity and good biocompatibility.
(8) The artificial purplish nanometer material prepared by the invention still has a part of active functional groups and can be post-modified. The photo-thermal reagent indocyanine green is loaded on the artificial purplish nanometer material, so that the light absorption capacity and the photo-thermal conversion performance of the artificial purplish nanometer material in a near infrared region can be further enhanced, and the problem of light stability of the photo-thermal reagent indocyanine green is solved; but also has good photo-thermal sterilization performance.
Drawings
FIG. 1 is a scanning electron micrograph of sample PHA;
FIG. 2 is a statistical value of particle size and yield of samples under different reaction times;
FIG. 3 shows the statistics of particle size for samples with different mass ratios of gentisic acid to sodium periodate;
FIG. 4 is a DPPH radical scavenging curve for sample PHA;
FIG. 5 is a UV-VIS absorption spectrum of a sample PHA;
FIG. 6 is a scanning electron micrograph of sample PHA-ICG;
FIG. 7 is a UV-VIS absorption spectrum of sample PHA and sample PHA-ICG;
FIG. 8 is a graph of the temperature increase and decrease of the PHA sample, the PHA-ICG sample, and the ICG sample;
FIG. 9 is the 24h cell compatibility of sample PHA and sample PHA-ICG;
FIG. 10 is a statistical plot of the cell-level antioxidant fluorescence intensity of sample PHA;
FIG. 11 is a graph of photothermocidal properties of samples PHA and PHA-ICG measured on E.coli;
FIG. 12 is a graph of photothermal bactericidal properties of sample PHA and sample PHA-ICG against Staphylococcus aureus.
Detailed Description
The technical solution and the technical effect of the present invention will be further explained with reference to the following embodiments. The examples are only for illustrative purposes and are not intended to limit the scope of the present invention. The technical solution of the present invention is further described below with reference to specific examples, but the present invention is not limited to the specific examples, and the present invention has many applications besides the specific examples.
The following examples and comparative examples used starting materials: the purity of the gentisic acid is 97.0 percent, and the gentisic acid is purchased from Shanghai Chiai chemical industry development limited company; sodium periodate with the purity of 99.8 percent, wherein the sodium periodate and the ethanol are purchased from Chengdu Konglong chemical products, Inc.; indocyanine green with a purity of 99.0% was purchased from Annaige chemical Co., Ltd.
Example 1
The specific steps for preparing the artificial purplish melanin nano material in the embodiment are as follows:
(1) and (2) taking deionized water containing ethanol as a solvent (wherein the weight of the ethanol accounts for 25%), preparing a 3mg/mL gentisic acid solution, and carrying out ultrasonic treatment on the gentisic acid solution for 5 minutes to uniformly disperse the gentisic acid. Then, the mixture was stirred at room temperature for 5 minutes to obtain a transparent colorless gentisic acid solution, and the stirring was maintained at room temperature for further use.
(2) Deionized water is used as a solvent to prepare a sodium periodate solution with the concentration of 214mg/mL, and the solution is kept stirring under the water bath condition of 50 ℃ for standby.
(3) And (3) dropwise adding a sodium periodate solution into the pergentisic acid solution, wherein the transparent and colorless solution is gradually changed into reddish brown and gradually turned deep in the dropwise adding process and is in a turbid state, and when a uniform and stable dark brown turbid solution is obtained, the reaction is finished.
(4) And (3) centrifuging the reaction solution, setting the rotating speed of a centrifuge to be 15000r/min and the centrifuging time to be 8 minutes to obtain a dark brown solid, and washing the dark brown solid with deionized water for three times to obtain an artificial purplish melanin nano material sample.
In this example, 7 groups of artificial purplish nanomaterial samples were prepared, and 7 groups of samples adopt different mass ratios of gentisic acid to sodium periodate and reaction time, as detailed in table 1, wherein sample 1 is named as sample PHA.
TABLE 17 Mass ratio of Gentianic acid to sodium periodate and reaction time for the samples in the group
| Numbering | Mass ratio of gentisic acid to sodium periodate | Reaction time |
| 1 | 6：7 | 24h |
| 2 | 6：11.5 | |
| 3 | 6：15.5 | |
| 4 | 6：7 | |
| 5 | 6：7 | |
| 6 | 6：7 | 16h |
| 7 | 6：7 | 48h |
Example 2
In this example, a desktop scanning electron microscope was used to observe the microstructure of PHA sample.
Preparing the sample PHA into a sample PHA solution with the concentration of 1mg/mL, spin-coating the sample PHA solution on the surface of a smooth mica sheet, and observing the sample PHA solution after drying and gold spraying treatment in sequence. The scanning electron micrograph is shown in FIG. 1. As can be seen from FIG. 1, the PHA sample is a nanoparticle with good morphology and uniform particle size, and the statistical particle size range is 147 nm-165 nm.
Example 3
This example performs particle size and yield statistics on the samples in table 1, and uses Image J software to measure the particle size of the samples from the sem pictures and make statistics on the average value.
The mass ratio of the PHA to the sodium periodate in the samples of 4-7 is 6: and 7, the reaction time is 24h, 8h, 12h, 16h and 48h respectively. The particle size and yield of PHA, 4-7 samples were tested and counted, see FIG. 2. As can be seen from FIG. 2, the particle size and yield of the sample both appeared to be stable after increasing with the reaction time, and the particle size and yield of the sample became stable after 24 h.
The reaction time of the sample PHA and the samples 2-3 is 24h, and the mass ratio of the gentisic acid to the sodium periodate is 6: 7. 6: 11.5, 6: 15.5. and testing and counting the particle sizes of the samples 1-3, wherein the statistic value of the particle sizes is shown in figure 3. As can be seen from fig. 3, the particle size of the sample tends to increase and then to become stable with the increase of the oxidizing agent sodium periodate. In FIGS. 2 to 3, the particle diameters are all average particle diameters.
Example 4
This example uses the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method to assess the in vitro DPPH free radical scavenging ability of sample PHA.
Respectively preparing a DPPH ethanol solution with the concentration of 0.1mmol/L and a sample ethanol phase solution with the concentration of 1 mg/mL. mu.L of the DPPH solution was diluted with an appropriate amount of ethanol, and 150. mu.L of the sample ethanol phase solution was added to maintain the final volume of the solution at 3mL, resulting in a sample solution with a concentration of 50. mu.g/mL. mu.L of DPPH solution was diluted with an appropriate amount of ethanol, and 300. mu.L of the sample ethanol phase solution was added to maintain the final volume of the solution at 3mL, resulting in a sample solution with a concentration of 100. mu.g/mL. The radical clearance rate is measured for two groups of sample solutions with different concentrations.
The absorbance at 517nm was used to evaluate the radical scavenging effect to evaluate the ethanol phase antioxidant capacity of the sample PHA. The absorbance was measured at various time points over 30 minutes to obtain a free radical scavenging curve, as shown in FIG. 4. The free radical scavenging curve can be used for representing the antioxidant capacity of the sample, and the higher the scavenging rate is, the stronger the antioxidant capacity of the sample is. As can be seen from FIG. 4, the samples showed enhanced increase in antioxidant gain with clearance rates up to approximately 80% as the sample concentration increased.
Example 5
This example measures the absorption capacity of a sample PHA for the UV-Vis absorption spectrum.
Preparing a sample PHA into an aqueous solution with the concentration of 50 mug/mL, and measuring the ultraviolet absorption of the sample PHA in the wavelength range of 200-900 nm by adopting an ultraviolet-visible spectrophotometer, wherein the width of a slit is 2 nm. The obtained ultraviolet-visible light absorption spectrum is shown in fig. 5, and as can be seen from fig. 5, the artificial melanin is similar to melanin in nature, and has the ultraviolet absorption capacity of the spectrum, which is also the characteristic of melanin materials.
Example 6
In this example, a photothermal agent indocyanine green is loaded on a sample PHA, and a specific method for loading indocyanine green is as follows:
preparing a sample water solution with the concentration of 1mg/mL, and preparing an indocyanine green water solution with the concentration of 1 mg/mL. And adding 100 mu L of indocyanine green aqueous solution into 1mL of sample aqueous solution, oscillating for 24h at constant temperature of 25 ℃ by a shaking table at constant speed, and loading the indocyanine green on the surface of the artificial purplish melanin through rich pi-pi interaction. And then centrifuging the reaction solution, setting the rotating speed of a centrifuge to 15000r/min and the centrifuging time to 8 minutes to obtain a dark brown solid, washing the dark brown solid with deionized water for three times to obtain an indocyanine green-loaded pustulosa nano material sample, and naming the sample as PHA-ICG. The loading of indocyanine green in sample PHA-ICG was 25 μ g/mg.
The PHA-ICG sample was characterized by a bench-top scanning electron microscope, the photograph of which is shown in FIG. 6. As can be seen from fig. 6, the loading of indocyanine green hardly affects the original morphology and particle size of the artificial puronone nanoparticles. The absorption capacity of the sample PHA-ICG in the wavelength range of 400nm-900nm is characterized by an ultraviolet-visible spectrophotometer, and the obtained ultraviolet-visible light absorption spectrum curve is shown in figure 7. As can be seen from fig. 7, in a near-infrared region, after indocyanine green is loaded, the light absorption capacity of the sample PHA-ICG is greatly improved, which provides a necessary condition for the application of the artificial pustulan nano material in infrared light.
In the invention, the photothermal conversion capability of the sample PHA-ICG is tested by a temperature rise and fall curve. The specific method comprises the following steps: preparing a sample PHA solution with the concentration of 100 mu g/mL, preparing a sample PHA-ICG solution with the concentration of 100 mu g/mL, and preparing an indocyanine green aqueous solution with the concentration of 2.5 mu g/mL, wherein the indocyanine green aqueous solution is recorded as the ICG solution. Placing 1mL of the sample in a test dish, irradiating for 600 seconds by using a near infrared light lamp with the power of 2.0W and the wavelength of 808nm to enable the temperature to rise to an extreme value, and then turning off a light source of the near infrared light lamp for 1200 seconds to enable the initial temperature to be recovered. This was repeated three times, with the real-time temperature recorded every 10 seconds using the UT320 software, to obtain the temperature ramp curve shown in fig. 8.
As can be seen from fig. 8, compared with the sample PHA, the photothermal conversion capability of the sample PHA-ICG is significantly improved, which indicates that the indocyanine green loaded can effectively improve the photothermal conversion capability of the artificial melanoidin nanomaterial. After three cycles, the photothermal conversion capacity of the indocyanine green is obviously attenuated, which shows the light instability of the indocyanine green, but the sample PHA-ICG has no attenuation after three cycles, which proves that the artificial pustulan nano material has a positive effect on improving the light stability of the indocyanine green.
From the embodiment, a certain amount of indocyanine green is loaded, so that the absorption capacity and the photo-thermal conversion capacity of the artificial puratin nano material in a near-infrared light region can be improved. Through adjusting the load capacity of the indocyanine green and then carrying out light absorption capacity characterization, the load capacity of the indocyanine green is better when 10 mu g/mg-30 mu g/mg and the best load capacity is 25 mu g/mg through the spectrum change of a near infrared region.
Example 7
The samples PHA and PHA-ICG were tested for biocompatibility. And (3) verifying the cytotoxicity of the sample PHA and the sample PHA-ICG by using an Alma blue test method by taking an NIH mouse embryo fibroblast 3T3 cell as a cell strain. The cells were cultured by adding 10% Fetal Bovine Serum (FBS) to DMEM medium and incubating the cells in an atmosphere containing 5% CO2The temperature was maintained at 37 ℃. Cultured NIH 3T3 cells were incubated in 96-well plates at a density of 2000 cells per well for 24h, further treated with different concentrations of sample PHA and sample PHA-ICG for 24h, and then tested for corresponding cell viability according to the Almarman blue test instructions, the results of which are shown in FIG. 9. As can be seen from fig. 9, the artificial pustulan nano material and the artificial pustulan nano material loaded with indocyanine green have good cell compatibility in a wide concentration range (50-500 μ g/mL), and a good foundation is laid for biological application of the artificial pustulan nano material and the artificial pustulan nano material.
Example 8
The samples PHA were tested for antioxidant capacity at the cell level.
The NIH 3T3 cells cultured in example 7 were seeded at 20 ten thousand per well in 6-well plates and incubated in the plates for 24 hours. After incubation, the culture medium is removed, 500 mu L of samples PHA with different concentrations prepared by DMEM culture medium are added, 1500 mu L of full-component culture solution and 200 mu L of diluted hydrogen peroxide are supplemented, and the concentration of the diluted hydrogen peroxide is 100 mu mol/L. The culture was then removed and 500. mu.L of trypsinized cells were added and 500. mu.L of medium was added to stop the trypsinization. Further centrifuging to remove supernatant, adding 1ml of PBS solution for resuspension, and adding 250 μ L of prepared probe; and finally, centrifuging the cell sample, then resuspending the cell sample in PBS, and carrying out quantitative analysis by using a flow cytometer for 24h to obtain a cell-level antioxidant fluorescence intensity statistical graph shown in FIG. 10.
In fig. 10, NC represents a negative control group to which hydrogen peroxide was not added; PC represents a positive control group which is only added with hydrogen peroxide and is not added with any material; PHA-200 represents an experimental group added with hydrogen peroxide and sample PHA, wherein the concentration of the sample PHA is 200 mug/mL; PHA-500 represents the experimental group with hydrogen peroxide added with sample PHA, wherein the concentration of the sample PHA is 500 mug/mL. As can be seen from FIG. 10, the fluorescence intensity of the positive control group stimulated by hydrogen peroxide is significantly increased, the fluorescence intensity is correspondingly decreased after the artificial melanin nano-material is added, and the decrease trend is more obvious along with the increase of the concentration, which proves that the artificial melanin nano-material has good oxidation resistance.
Example 9
And testing the photothermal sterilization performance of the sample PHA and the sample PHA-ICG.
Before the test, the reagent and the test tube required by the test are disinfected. The antibacterial activity of the bacteria was evaluated by the diffusion plate method. Coli and s.aureus were used as models, respectively. Taking 10 mu L of cell suspension as an exponential growth stage, adding the cell suspension into test tubes containing 2mL of sterile LB liquid culture medium, and adding different amounts of PHA and ICG samples into different test tubes respectively to ensure that the PHA and ICG samples have different sample concentrations in a bacterial solution. The bacteria solution was irradiated with 808nm near infrared light source with power of 2W for 10 min. And after the irradiation is finished, measuring the absorbance of the sample at the wavelength of 600nm by using an enzyme-labeling instrument, recording the OD value, further calculating the bacterial activity statistics according to the OD value, wherein the statistical value is shown in figures 11-12, wherein figure 11 corresponds to escherichia coli, and figure 12 corresponds to staphylococcus aureus. In FIG. 11, PHA NIR indicates that the sample PHA is added while being irradiated with a 808nm near-infrared light source with a power of 2W for 10 min; PHA-ICG NIR means that the sample PHA-ICG is added and simultaneously the sample PHA-ICG is irradiated for 10min by a near infrared light source with the power of 2W and the wavelength of 808 nm. As can be seen from FIGS. 11 to 12, the sample has a weak bacteria inhibition effect without an external infrared light source; under the irradiation of an infrared light source, the inhibition effect of bacteria is obviously enhanced, and the inhibition effect is more obvious along with the increase of the concentration of a sample.
The foregoing are merely exemplary embodiments of the present invention, which enable those skilled in the art to understand or practice the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A preparation method of an artificial purplish melanin nano material is characterized by comprising the following steps:
preparing a sodium periodate solution and a gentisic acid solution, dropwise adding the sodium periodate solution into the gentisic acid solution, reacting for 8-24 hours, and sequentially performing centrifugal treatment and deionized water washing on the reaction solution to obtain an artificial pustulan nano material; wherein the dosage of the sodium periodate and the dosage of the gentisic acid are respectively 6 parts by weight and 2-20 parts by weight.
2. The method for preparing artificial melanin nano-material according to claim 1, which is characterized in that:
the using amounts of the sodium periodate and the gentisic acid are respectively 6 parts by weight and 7-15.5 parts by weight.
3. The method for preparing artificial pustulan nano material according to claim 1, which is characterized in that:
the using amounts of the sodium periodate and the gentisic acid are respectively 6 parts by weight and 7-11.5 parts by weight.
4. The method for preparing artificial melanin nano-material according to claim 1, which is characterized in that:
the use amounts of the sodium periodate and the gentisic acid are respectively 6 parts by weight and 7 parts by weight.
5. The method for preparing artificial melanin nano-material according to claim 1, which is characterized in that:
the sodium periodate solution is prepared by taking an aqueous solution containing ethanol as a solvent, wherein the weight percentage of the ethanol in the aqueous solution is 10-40%, and the concentration of the prepared sodium periodate solution is 2-4 mg/mL; and the gentisic acid solution is prepared by taking deionized water as a solvent, and the concentration of the gentisic acid solution is 100-300 mg/mL.
6. The method for preparing artificial melanin nano-material according to claim 1, which is characterized in that:
further comprising: the photo-thermal reagent indocyanine green is loaded on the artificial purplish melanin nano material, and the loading capacity is 10-30 mug/mg.
7. Use of the artificial melanin nanomaterial prepared by the preparation method of any one of claims 1 to 6 as an antioxidant material.
8. Use of the artificial melanin nanomaterial prepared by the preparation method of any one of claims 1 to 6 as a light absorbing material.
9. Use of the artificial melanoidin nanomaterial produced by the production method according to any one of claims 1 to 6 as a photothermal conversion material.
10. The use of the artificial melanin nanomaterial prepared by the preparation method of any one of claims 1 to 6 as a photothermal sterilization material.
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| CN115120741A (en) * | 2022-07-08 | 2022-09-30 | 四川大学 | Preparation method and application of melanin-sanshool composite nano material |
| CN115969974A (en) * | 2022-12-29 | 2023-04-18 | 重庆医科大学附属第二医院 | Melanoid bionic nanoparticles, preparation method and application thereof in-situ glioblastoma photothermal immunotherapy |
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| CN115969974A (en) * | 2022-12-29 | 2023-04-18 | 重庆医科大学附属第二医院 | Melanoid bionic nanoparticles, preparation method and application thereof in-situ glioblastoma photothermal immunotherapy |
| CN115969974B (en) * | 2022-12-29 | 2024-05-28 | 重庆医科大学附属第二医院 | Isoalanin bionic nanoparticle, preparation method and application thereof in photothermal immunotherapy of glioblastoma in situ |
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