CN114292893B - Method for shortening fermentation period and improving acid yield by adding various microelements into VC mixed strain seed tank - Google Patents
Method for shortening fermentation period and improving acid yield by adding various microelements into VC mixed strain seed tank Download PDFInfo
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Abstract
The invention discloses a method for shortening a fermentation period and improving acid yield by adding various microelements into a VC mixed strain seed tank, belonging to the technical field of biological medicine, comprising seed tank fermentation and fermentation tank fermentation; fermenting in the seed tank, sterilizing the fermentation medium after preparing the fermentation medium, transferring the fermentation medium into the seed tank, inoculating with the inoculating liquid, culturing after inoculating, and obtaining the seed liquid after culturing; the content of microelements in the fermentation medium in the seed tank is 18-22mL/L; according to the invention, trace elements are added into the formula of the seed tank in the mixed bacteria fermentation system, so that the small bacteria can grow rapidly before the large bacteria produce spores, thereby shortening the period of the seed tank, and simultaneously adjusting the proportion of the large bacteria to the small bacteria, thereby being beneficial to shortening the fermentation period and improving the yield of gulonic acid; meanwhile, the formula and the sterilization mode of the seed tank are adjusted, so that the seed tank is more beneficial to the growth of thalli.
Description
Technical Field
The invention belongs to the technical field of biological medicine, and particularly relates to a method for shortening a fermentation period and improving acid yield by adding various microelements into a VC mixed strain seed tank.
Background
In the 70 s of the last century, yin Guanglin et al, beijing institute of microbiology, department of Chinese sciences, invented a two-step fermentation method for bio-oxidative substitution chemical synthesis. The mixed bacteria system is fermented to replace the multi-step chemical reaction in the Lei method, wherein the mixed bacteria system comprises an acidogenic bacteria and an associated bacteria, the acidogenic bacteria are common ketogulonic bacteria, commonly called microbacteria, which grow slowly during independent culture, can be converted into 2-keto-L gulonic acid by taking sorbose as a substrate, but have low independent acidogenic capacity, and the associated bacteria commonly called macrophytes do not produce acid, but can obviously promote the growth and acidogenesis of the microbacteria during the mixed culture with the acidogenic bacteria.
Compared with the Lei method, the two-step fermentation method omits the use of dangerous chemical reagents, reduces the production cost of raw materials, equipment and the like, shortens the process flow and the fermentation period, is mostly liquid reaction, and is favorable for the continuity and the automation of production.
However, the two-step fermentation method still has the following problems: the vitamin C two-step fermentation method adopts mixed bacteria of different sizes for fermentation, the two strains belong to different bacteria, have different physiological characteristics and nutritional requirements, have complex symbiotic and metabolic relationship between the two bacteria, are difficult to determine the collocation proportion, are difficult to control, and severely restrict the acid production potential of the VC two-step mixed bacteria fermentation; by adopting a method of single-strain fermentation of acid-producing bacteria, the single-strain culture of the small bacteria is slow in growth and weak in acid production capacity, and although a method of relieving oxidative stress by adding foreign substances is provided, the problem of oxidative stress suffered by the single-strain fermentation of the small bacteria is still not solved at present; low conversion rate, more byproducts, difficult industrialization and the like.
Disclosure of Invention
The invention provides a method for shortening fermentation period and improving acid yield by adding various trace elements into a VC mixed strain seed tank, which is characterized in that trace elements are added into a seed tank formula in a mixed strain fermentation system, so that small bacteria can grow rapidly before the large bacteria produce spores, thereby shortening the period of the seed tank, adjusting the ratio of the large bacteria to the small bacteria, and being beneficial to shortening the fermentation period and improving the yield of gulonic acid; meanwhile, the formula and the sterilization mode of the seed tank are adjusted, so that the seed tank is more beneficial to the growth of thalli.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method for shortening fermentation period and increasing acid yield by adding multiple microelements into VC mixed strain seed tank comprises seed tank fermentation and fermentation tank fermentation.
The fermentation medium is sterilized after the fermentation medium is prepared, then the fermentation medium is moved into a seed tank, inoculated by using an inoculating liquid after the seed tank is cooled to 30 ℃, the air flow rate of the introduced liquid is controlled to be 1.3-1.7L/min, the rotating speed is 130-170rpm, the tank pressure is 0.015-0.025MPa, the culture temperature is 28-32 ℃, the inoculum size of the inoculating liquid is 1.8-2.2%, sampling and detection are carried out every 1.8-2.2h, and the culture is ended to obtain the seed liquid after the acid value reaches 2.8-3.2 g/L.
The formula of the fermentation medium in the seed tank comprises 9-11% of sorbose, 1.8-2.2% of corn steep liquor, 0.018-0.022% of magnesium sulfate, 0.18-0.22% of monopotassium phosphate, 0.18-0.22% of urea, 0.08-0.12% of calcium carbonate, 18-22mL/L of trace elements and the balance of primary water.
The pH of the fermentation medium in the seed tank is 7.6-7.8.
The formula of the trace elements comprises the following components: 0.018-0.022g/L of reduced glutathione, 0.018-0.022g/L of threonine, 0.008-0.012g/L of vitamin B, 0.008-0.012g/L of dihydrofolate, 0.18-0.22g/L of lysine, 4.8-5.2g/L of methionine, 0.38-0.42g/L of methionine, 0.08-0.12g/L of lipoic acid, 0.023-0.027g/L of vitamin B and the balance of primary water.
When the fermentation medium in the seed tank is sterilized, a method of separately sterilizing the sorbose is adopted, and the sorbose is sterilized for 18-22min at 115-120 ℃; then placing the other components in the seed tank into a sterilizing tank, sterilizing at 118-122 deg.C for 18-22min, and mixing with sorbose.
The inoculation liquid formula comprises 0.018-0.022 part of magnesium sulfate, 0.08-0.12 part of monopotassium phosphate, 0.08-0.12 part of urea, 1.4-1.6 parts of corn dry powder, 2.8-3.2 parts of yeast extract,18-22 parts of sorbose, 100cfu/mL of bacillus megatherium and 3 x 10 of gluconobacter oxydans 6 cfu/mL。
The bacillus megaterium is classified and named as Bacillus megaterium, the gluconobacter oxydans is classified and named as Gluconobacter oxydans, and all are purchased from the market.
And (3) fermenting in the fermentation tank, transferring the seed liquid into the fermentation tank for continuous fermentation, controlling the initial inoculation amount to be 16-20%, controlling the PH at the time of fermentation to be 6.8-7.2, controlling the culture temperature to be 28-32 ℃, controlling the rotating speed to be 290-310rpm, feeding a sorbic sugar water solution, controlling the sugar content to be 13-17g/L in the process, and ending the fermentation when the acid value of gulonic acid reaches 120-140g/L and the residual sugar is reduced to be less than 0.2 g/L.
The formula of the fermentation medium in the fermentation tank comprises, by weight, 0.8-1.2 parts of urea, 0.18-0.22 parts of magnesium sulfate, 0.18-0.22 parts of monopotassium phosphate, 7-7.4 parts of corn dry powder, 18-22 parts of sorbose and the balance of tap water.
The initial concentration of the sorbose is 18-22g/L, and the sorbose is supplemented with aqueous solution of sorbose by adopting a fed-batch manner in fermentation.
The concentration of the aqueous solution of sorbose is 500g/L.
The sorbose in the fermentation medium in the fermentation tank is sterilized by an independent sterilizing pot, the sterilizing temperature is 116-120 ℃, and the sterilizing time is 18-22min; the other fermentation medium components in the fermentation tank are mixed after being prepared, and then steam sterilization is carried out in the fermentation tank, wherein the sterilization temperature is 113-117 ℃, and the sterilization time is 13-17min.
Compared with the prior art, the invention has the beneficial effects that:
(1) According to the method for improving the acid yield by adding various microelements into the VC mixed strain seed tank, the microelements are added to enable the small bacteria to enter the growing period in advance, so that the small bacteria amount of the seed tank is improved, and the fermentation period of the seed tank is shortened by 4-6 hours;
(2) According to the method for improving the acid yield by adding various microelements into the VC mixed strain seed tank, disclosed by the invention, the ratio of the big and small bacteria in the inoculation liquid is adjusted, so that the small bacteria can enter the fast growth period in advance, and meanwhile, the growth state of the small bacteria is good;
(3) The method for shortening the fermentation period and improving the acid yield by adding various microelements into the VC mixed strain seed tank can shorten the period of the fermentation tank by 2 hours;
(4) The method for improving the acid yield by adding various microelements into the VC mixed strain seed tank shortens the fermentation period, and can improve the molar yield of the fermentation tank by 1.05% -1.34%.
Detailed Description
Specific embodiments of the present invention will now be described in order to provide a clearer understanding of the technical features, objects and effects of the present invention.
Example 1
1. Four 5L seed tanks are selected to be tightly combined into a group, namely a 1# tank, a 2# tank, a 3# tank and a 4# tank, and the four seed tanks are not communicated. Preparing a culture medium in a seed tank according to a two-step seed tank formula, wherein the culture medium formula of a 3# seed tank is as follows:
10% of sorbose, 2% of corn steep liquor, 0.02% of magnesium sulfate, 0.2% of monopotassium phosphate, 0.2% of urea, 0.1% of calcium carbonate, 20mL/L of trace elements and the balance of primary water.
The seed tank is prepared by adopting primary water, the constant volume is 3.5L, and the PH is adjusted to 7.6-7.8.
The culture medium formulations of the four seed tanks are different in that: no trace elements are added in the No. 1 tank; adding 10ml/L trace elements into a No. 2 tank; adding 20ml/L trace elements into a 3# tank; the trace element was added in a 30ml/L tank 4.
The formula of the trace elements comprises the following components: 0.02g/L of reduced glutathione, 0.02g/L of threonine, 0.01g/L of vitamin B6, 0.01g/L of dihydrofolate, 0.2g/L of lysine, 5g/L of methionine, 0.4g/L of methionine, 0.1g/L of lipoic acid, 0.025g/L of vitamin B and the balance of primary water.
2. And (3) sterilization: the seed tank adopts a method of separately sterilizing sorbose in the preparation process, and the sorbose is sterilized for 20min at 118 ℃.
Placing the other components in the seed tank into a sterilizing tank, sterilizing at 120deg.C for 20min, and mixing with sorbose.
3. And (3) process control: and (3) inoculating after cooling to 30 ℃ in the seed tank, controlling the air flow rate of the introduced seed tank to be 1.5L/min, the rotating speed to be 150rpm, the tank pressure to be 0.02MPa, the culture temperature to be 30 ℃ and the inoculation amount to be 2%.
The formula of the inoculation liquid comprises the following steps: 0.02 part of magnesium sulfate, 0.1 part of monopotassium phosphate, 0.1 part of urea, 1.5 parts of corn dry powder, 3 parts of yeast extract, 20 parts of sorbose, 100cfu/mL of bacillus megaterium bacterial amount and 3 x 10 of gluconobacter oxydans bacterial amount 6 cfu/mL。
The bacillus megaterium is classified and named as Bacillus megaterium, the gluconobacter oxydans is classified and named as Gluconobacter oxydans, and all are purchased from the market.
4. Seed pot data detection: the culture temperature of the seed tank is controlled to be 30 ℃, the tank pressure is controlled to be 0.04MPa, the rotating speed is 150rpm, sampling and detection are carried out every 2 hours, and the culture is ended when the acid value reaches 3 g/L.
5. Seed pot results: the cycle of the acid value of the No. 1 seed tank reaches 3.0g/L, the cycle of the acid value of the No. 2 seed tank reaches 3.0g/L, the cycle of the acid value of the No. 3 seed tank reaches 32h, the cycle of the acid value of the No. 3 seed tank reaches 3.0g/L, the cycle of the acid value of the No. 3 seed tank reaches 28h, and the cycle of the acid value of the No. 4 seed tank reaches 3.0g/L, and the cycle of the acid value of the No. 4 seed tank reaches 28h.
Example 2
The 3# tank experiment condition was repeated with the same formulation and culture conditions, and the acid value of the seed tank reached 3.0g/L with a period of 26 hours.
Example 3
1. Two sets of 10L seed tanks and 50L fermentation tanks are selected, namely a 1# seed tank (10L), a 1# fermentation tank (50L) and a 2# seed tank (10L) and a 2# fermentation tank (50L), wherein the 1# seed tank and the 1# fermentation tank are tightly connected, the 2# seed tank and the 2# fermentation tank are tightly connected, and the two seed tanks are not communicated with each other and the two fermentation tanks are not communicated with each other.
20ml/L trace elements are added into the culture medium of a No. 1 seed tank, and no trace elements are added into a No. 2 seed tank;
wherein, the formula of the culture medium of the 1# tank and the 2# tank is the same as that of the example 1, and the composition of microelements is the same as that of the example 1.
2. Preparing an inoculation liquid, controlling the composition of the inoculation liquid to be the same as that of the example 1, respectively inoculating the inoculation liquid into a 1# seed tank and a 2# seed tank filled with a fermentation culture medium, controlling the flow rate of air to be 1.5L/min, the rotating speed to be 150rpm, the culture temperature to be 30 ℃, the inoculation amount to be 2%, and ending fermentation when the OD is more than 3 and the acid value of gulonic acid is more than 5 g/L;
3. and respectively transferring the seed liquid in the seed tank 1 into the fermentation tank 1 filled with the fermentation medium through a seed transfer pipeline, transferring the seed liquid in the seed tank 2 into the fermentation tank 2 filled with the fermentation medium, controlling the initial inoculation amount to be 18%, controlling the pH at 7.0 during fermentation, controlling the culture temperature to be 30 ℃, controlling the rotating speed to be 300rpm, feeding the aqueous solution of sorbose, controlling the sugar concentration during fermentation to be 13-17g/L, and ending the fermentation when the acid value of gulonic acid reaches 130g/L and the residual sugar is reduced to be less than 0.2 g/L.
The formula of the fermentation culture medium in the No. 1 fermentation tank and the No. 2 fermentation tank comprises, by weight, 1 part of urea, 0.2 part of magnesium sulfate, 0.2 part of monopotassium phosphate, 7.2 parts of corn dry powder, 20 parts of sorbose, and the balance of tap water, wherein the volume is fixed to 20L.
The initial concentration of the sorbose is 20g/L, and the sorbose is supplemented with an aqueous solution of sorbose by adopting a feeding way in fermentation.
The concentration of the aqueous solution of sorbose is 500g/L.
The sorbose and the aqueous solution of sorbose are sterilized by an independent sterilizing pot, the sterilizing temperature is 118 ℃, and the sterilizing time is 20min; the other fermentation medium components in the fermenter were mixed after preparation, and then steam sterilization was performed in a 50L fermenter at 115℃for 15 minutes.
2. Experimental results: the acid value of the No. 1 reaches 135.68g/L, the period is 50h, and the yield is 93.48%; the acid value of the No. 2 reaches 133.62g/L, the period is 52h, and the yield is 92.14%.
The yield is the molar yield, which is the ratio of the molar total amount of gulonic acid to the molar total amount of sorbose consumed.
Example 4
Two batches of the example three experimental conditions were repeated using the same formulation and culture conditions, with the final experimental results shown below:
seed pot culture conditions and period:
then transferring into a fermentation tank for culturing, wherein the culture conditions and period are as follows:
the percentages used in the present invention are mass percentages unless otherwise indicated.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A method for shortening fermentation period and improving acid yield by adding various microelements into a VC mixed strain seed tank is characterized by comprising seed tank fermentation and fermentation in a fermentation tank;
the bacteria used in the fermentation are bacillus megateriumBacillus megaterium) And Gluconobacter oxydans [ ]Gluconobacter oxydans）；
The formula of the trace elements comprises the following components: 0.018-0.022g/L of reduced glutathione, 0.018-0.022g/L of threonine, 0.008-0.012g/L of vitamin B, 0.008-0.012g/L of dihydrofolate, 0.18-0.22g/L of lysine, 4.8-5.2g/L of methionine, 0.38-0.42g/L of methionine, 0.08-0.12g/L of lipoic acid, 0.023-0.027g/L of vitamin B and the balance of primary water.
2. The method for shortening fermentation period and improving acid yield by adding various microelements into a VC mixed seed tank according to claim 1, wherein the fermentation of the seed tank is completed, the fermentation medium is sterilized after the fermentation medium is prepared, then the fermentation medium is transferred into the seed tank, the seed tank is cooled to 30 ℃, then the seed tank is inoculated with an inoculating liquid, the introduced air flow is controlled to be 1.3-1.7L/min, the rotating speed is 130-170rpm, the tank pressure is 0.015-0.025MPa, the culture temperature is 28-32 ℃, the inoculating amount of the inoculating liquid is 1.8-2.2%, sampling and detection are carried out every 1.8-2.2h, and the culture is finished to obtain the seed liquid after the acid value reaches 2.8-3.2 g/L.
3. The method for improving the yield of acid by adding a plurality of trace elements into a VC mixed seed tank to shorten the fermentation period according to claim 2, wherein the formula of a fermentation medium in the seed tank comprises 9-11% of sorbose, 1.8-2.2% of corn steep liquor, 0.018-0.022% of magnesium sulfate, 0.18-0.22% of potassium dihydrogen phosphate, 0.18-0.22% of urea, 0.08-0.12% of calcium carbonate, 18-22mL/L of trace elements and the balance of primary water.
4. The method for improving the yield of the acid by adding a plurality of microelements into a VC mixed seed tank to shorten the fermentation period, which is characterized in that when a fermentation medium in the seed tank is sterilized, a method of separately sterilizing the sorbose is adopted, and the sorbose is sterilized for 18-22min at 115-120 ℃; then placing the other components in the seed tank into a sterilizing tank, sterilizing at 118-122 deg.C for 18-22min, and mixing with sorbose.
5. The method for improving the yield of acid by adding multiple trace elements into a VC mixed strain seed tank to shorten the fermentation period as claimed in claim 2, wherein the inoculating liquid comprises 0.018-0.022 part of magnesium sulfate, 0.08-0.12 part of monopotassium phosphate, 0.08-0.12 part of urea, 1.4-1.6 parts of corn dry powder, 2.8-3.2 parts of yeast extract, 18-22 parts of sorbose, 100cfu/mL of bacillus megaterium and 3 x 10 of gluconobacter oxydans 6 cfu/mL。
6. The method for shortening fermentation period and improving acid yield by adding various microelements into a VC mixed seed tank according to claim 1, wherein the fermentation in the fermentation tank is carried out, seed liquid is transferred into the fermentation tank for continuous fermentation, the initial inoculation amount is controlled to be 16-20%, the PH during fermentation is controlled to be 6.8-7.2, the culture temperature is 28-32 ℃, the rotating speed is 290-310rpm, a sorbose water solution is fed, the process sugar is controlled to be 13-17g/L, and the fermentation is ended when the acid value of gulonic acid reaches 120-140g/L and the residual sugar is reduced to be less than 0.2 g/L.
7. The method for shortening fermentation period and improving acid yield by adding multiple microelements into a VC mixed seed tank as claimed in claim 6, wherein the formula of the fermentation medium in the fermentation tank comprises, by weight, 0.8-1.2 parts of urea, 0.18-0.22 parts of magnesium sulfate, 0.18-0.22 parts of potassium dihydrogen phosphate, 7-7.4 parts of corn dry powder, 18-22 parts of sorbose and the balance tap water.
8. The method for shortening fermentation period and improving acid yield by adding multiple microelements into a VC mixed seed tank according to claim 7, wherein the initial concentration of sorbose is 18-22g/L, and the sorbose is supplemented with aqueous solution of sorbose by feeding.
9. The method for shortening fermentation period and improving acid yield by adding multiple microelements into a VC mixed seed tank according to claim 7, wherein the sorbose in a fermentation medium in the fermentation tank is sterilized by a single sterilizing pot at 116-120 ℃ for 18-22min; the other fermentation medium components in the fermentation tank are mixed after being prepared, and then steam sterilization is carried out in the fermentation tank, wherein the sterilization temperature is 113-117 ℃, and the sterilization time is 13-17min.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012174978A1 (en) * | 2011-06-20 | 2012-12-27 | 天津大学 | Strain improvement and process optimization in two-step mixed fermentation for production of vitamin c |
| CN103614424A (en) * | 2013-11-21 | 2014-03-05 | 宁夏启元药业有限公司 | Method of improving fermentation unit of gulconic acid |
| CN106434830A (en) * | 2016-12-20 | 2017-02-22 | 宁夏启元药业有限公司 | Method for improving 2-keto-L-gluconic acid fermentation efficiency |
| CN107955828A (en) * | 2017-11-28 | 2018-04-24 | 宁夏启元药业有限公司 | A kind of Vitamin C Two-step Fermentation production method |
| CN110760468A (en) * | 2019-11-29 | 2020-02-07 | 宁夏启元药业有限公司 | Method for improving efficiency of vitamin C precursor 2-keto-L-gulonic acid |
| CN110938564A (en) * | 2019-12-05 | 2020-03-31 | 石药集团维生药业（石家庄）有限公司 | Method for promoting growth and metabolism of ketogenic gulonospora |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012174978A1 (en) * | 2011-06-20 | 2012-12-27 | 天津大学 | Strain improvement and process optimization in two-step mixed fermentation for production of vitamin c |
| CN103614424A (en) * | 2013-11-21 | 2014-03-05 | 宁夏启元药业有限公司 | Method of improving fermentation unit of gulconic acid |
| CN106434830A (en) * | 2016-12-20 | 2017-02-22 | 宁夏启元药业有限公司 | Method for improving 2-keto-L-gluconic acid fermentation efficiency |
| CN107955828A (en) * | 2017-11-28 | 2018-04-24 | 宁夏启元药业有限公司 | A kind of Vitamin C Two-step Fermentation production method |
| CN110760468A (en) * | 2019-11-29 | 2020-02-07 | 宁夏启元药业有限公司 | Method for improving efficiency of vitamin C precursor 2-keto-L-gulonic acid |
| CN110938564A (en) * | 2019-12-05 | 2020-03-31 | 石药集团维生药业（石家庄）有限公司 | Method for promoting growth and metabolism of ketogenic gulonospora |
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