CN114292794A - Pediococcus pentosaceus SD22 and screening method and application thereof - Google Patents
Pediococcus pentosaceus SD22 and screening method and application thereof Download PDFInfo
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Abstract
The invention provides pediococcus pentosaceus SD22 and a screening method and application thereof, and relates to the technical field of microorganisms. The pediococcus pentosaceus SD22 provided by the invention has a preservation number of CGMCC No.19327, is screened from farmhouse pickles in big summer mountains in Sichuan, has good safety, has good inhibition effect on food-borne pathogenic bacteria including salmonella and escherichia coli, has good application potential in the aspect of preventing and treating the food-borne pathogenic bacteria, can be used for preparing products for preventing and treating the food-borne pathogenic bacteria, and is a probiotic strain worthy of development.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to pediococcus pentosaceus SD22, a screening method and application thereof.
Background
Salmonella is a common food-borne pathogenic bacterium and widely exists in meat products, egg products, carriers such as soil, water and the like and the environment. After animals and people are infected by salmonella, the animals and the people can suffer from salmonellosis, which not only brings huge economic loss to the breeding industry, but also threatens human health. Coli is also one of the main causes of food-borne diseases, and in recent years, frequent food pathogenic bacteria events at home and abroad have become a major problem threatening the health of people. Therefore, the practical and effective method is adopted to kill or inhibit the food-borne pathogenic bacteria salmonella and escherichia coli to ensure the safety of food is imminent.
Pediococcus pentosaceus (Pediococcus pentosaceus) is one of the strains that can be used in common food. At present, the probiotic function of pediococcus pentosaceus and its application in fermented foods have been highly appreciated by researchers in the field of food science. A large number of researches show that pediococcus pentosaceus has certain functions in the aspects of inhibiting food-borne diseases, regulating intestinal immunity, reducing cholesterol, resisting tumors and the like, so that the pediococcus pentosaceus has great value in the field of food industry when being applied to control the food-borne diseases caused by salmonella and escherichia coli.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The first purpose of the invention is to provide pediococcus pentosaceus SD22, which is preserved in China general microbiological culture Collection center in 1 month and 10 months in 2020 with the preservation numbers as follows: CGMCC No. 19327.
The second object of the present invention is to provide a method for screening Pediococcus pentosaceus SD22, which is simple and convenient.
The third purpose of the invention is to provide a microbial inoculum.
It is a fourth object of the present invention to provide a fermentation product.
The fifth purpose of the invention is to provide the application of the pediococcus pentosaceus SD22, the microbial inoculum or the fermentation product in the preparation of products for preventing and treating food-borne diseases.
The sixth purpose of the invention is to provide a product for preventing and treating oral pathogenic bacteria.
In a first aspect, the invention provides pediococcus pentosaceus SD22, wherein the pediococcus pentosaceus SD22 is deposited in the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms with the following deposition numbers: CGMCC No. 19327.
In a second aspect, the present invention provides a screening method for pediococcus pentosaceus SD22, comprising the following steps:
separating and purifying the strain to obtain a single colony, and then identifying the single colony to obtain pediococcus pentosaceus SD 22;
the strain is sampled from agricultural pickled vegetables in big summer mountains in Sichuan.
As a further technical scheme, the separation and purification method comprises a coating flat plate method and a plate scribing method.
As a further technical scheme, the identification method comprises a physiological and biochemical test, a 16S r RNA gene sequence analysis method and a pheS gene sequence analysis method.
In a third aspect, the invention provides a bacterial agent, comprising pediococcus pentosaceus SD 22.
As a further technical scheme, the preparation formulation of the microbial inoculum comprises liquid microbial inoculum, powder and granular microbial inoculum.
In a fourth aspect, the invention provides a fermentation product obtained by fermenting pediococcus pentosaceus SD22 or a microbial inoculum.
In a fifth aspect, the invention provides application of pediococcus pentosaceus SD22, a microbial inoculum or a fermentation product in preparation of products for preventing and treating food-borne pathogenic bacteria.
As a further technical scheme, the food-borne pathogenic bacteria comprise salmonella and escherichia coli.
In a sixth aspect, the invention provides a product for preventing and treating food-borne pathogenic bacteria, which comprises pediococcus pentosaceus SD22, a microbial inoculum or a fermentation product.
Compared with the prior art, the invention has the following beneficial effects:
the pediococcus pentosaceus SD22 provided by the invention is derived from agricultural pickled vegetables in great mountains in Sichuan, has good safety, has a good inhibition effect on food-borne pathogenic bacteria including salmonella and escherichia coli, has good application potential in the aspect of preventing and treating the food-borne pathogenic bacteria, has a wide prospect, can be used for preparing products for preventing and treating the food-borne pathogenic bacteria, and is a probiotic strain worthy of development.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 shows the calcium-solubilizing circle of the selected strain SD 22;
FIG. 2 is the colony morphology of strain SD 22;
FIG. 3 is a gram stain of strain SD 22;
FIG. 4 is a phylogenetic tree of strain SD22 based on the 16S r RNA sequence;
FIG. 5 is a growth curve of Pediococcus pentosaceus SD 22;
FIG. 6 is a graph showing the inhibition effect of Pediococcus pentosaceus SD22 on the inhibition of Salmonella by Oxfordii cup;
FIG. 7 is a graph showing the Oxford cup bacteriostatic effect of Pediococcus pentosaceus SD22 on Escherichia coli inhibition.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to embodiments and examples, but those skilled in the art will understand that the following embodiments and examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. Those who do not specify the conditions are performed according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In a first aspect, the present invention provides a pediococcus pentosaceus SD22, which is classified and named as: pediococcus pentosaceus, latin literature name: pediococcus pentosaceus, deposited in China general microbiological culture Collection center, with the deposition address: west road No.1 hospital No. 3, north jing, chaoyang district, preservation date: year 2020, month 1, day 10, accession number: CGMCC No. 19327.
The pediococcus pentosaceus SD22 is derived from farmyard pickles in big summer hills in Sichuan, and is obtained by colony morphology tests, and the pediococcus pentosaceus SD22 colony is milky white, neat in edge, convex, smooth in surface and uniform in texture. The shape of the thallus is spherical, the gram staining result is positive, and the thallus has no flagellum, no movement and no spores.
The research of the inventor finds that the pediococcus pentosaceus SD22 provided by the invention has a good inhibition effect on food-borne pathogenic bacteria including salmonella and escherichia coli, has good application potential in the aspect of prevention and treatment of the food-borne pathogenic bacteria, and is a probiotic strain worthy of development.
In a second aspect, the present invention provides a screening method for pediococcus pentosaceus SD22, comprising the following steps:
and separating and purifying the bacterial strain sampled from the agricultural pickled vegetables in the great summer mountains in Sichuan to obtain a single bacterial colony, and then identifying the single bacterial colony to obtain pediococcus pentosaceus SD 22.
The screening method of pediococcus pentosaceus SD22 provided by the invention is simple and convenient, and can quickly obtain a plurality of strains including pediococcus pentosaceus SD 22.
In some preferred embodiments, the isolation and purification methods include, but are not limited to, spread plate and plate-streaking, or a strain isolation and purification method well known to those skilled in the art.
In some preferred embodiments, the methods of identification include physiological and biochemical assays, 16 sra gene sequence analysis, and pheS gene sequence analysis. The strain is preliminarily identified by physiological and biochemical tests. The species of the strain was obtained by 16S r RNA gene sequence analysis. The pheS gene sequence analysis method is adopted for identifying microbial strains, and the method can have a good distinguishing effect among certain strains, subspecies and strains.
In a third aspect, the invention provides a microbial inoculum, which comprises pediococcus pentosaceus SD22, and can also comprise other bacterial strains or auxiliary materials and the like, and the microbial inoculum has all the beneficial effects of the pediococcus pentosaceus SD 22.
In some preferred embodiments, the formulation of the microbial inoculum includes liquid microbial inoculum, powder microbial inoculum and granular microbial inoculum.
In a fourth aspect, the invention provides a fermentation product obtained by fermenting pediococcus pentosaceus SD22 or a microbial inoculum.
The research of the inventor finds that the fermentation product of pediococcus pentosaceus SD22, including food-borne pathogenic bacteria such as salmonella and escherichia coli, has a good inhibition effect and can be used for preparing antibacterial products.
In a fifth aspect, the invention provides application of pediococcus pentosaceus SD22, a microbial inoculum or a fermentation product in preparation of products for preventing and treating food-borne pathogenic bacteria.
The research of the inventor finds that the pediococcus pentosaceus SD22, the microbial inoculum or the fermentation product provided by the invention have obvious bacteriostatic effect on food-borne pathogenic bacteria including salmonella and escherichia coli, and therefore, the pediococcus pentosaceus SD22, the microbial inoculum or the fermentation product can be used for preparing products for preventing and treating the food-borne pathogenic bacteria.
In a sixth aspect, the invention provides a product for preventing and treating food-borne pathogenic bacteria, which comprises pediococcus pentosaceus SD22, a microbial inoculum or a fermentation product.
The research of the inventor finds that the pediococcus pentosaceus SD22, the microbial inoculum or the fermentation product provided by the invention have an obvious bacteriostatic effect on food-borne pathogenic bacteria including salmonella and escherichia coli, so that the medicine including the pediococcus pentosaceus SD22, the microbial inoculum or the fermentation product also has the effect of inhibiting the food-borne pathogenic bacteria, and can be used for preventing and treating the food-borne pathogenic bacteria.
The invention is further illustrated by the following specific examples and comparative examples, but it should be understood that these examples are for purposes of illustration only and are not to be construed as limiting the invention in any way.
Unless otherwise specified, the experimental procedures referred to in the following examples are those conventional in the art, and the reagents or equipment referred to are commercially available from a normal source.
The species and medium used in the test are shown in Table 1.
TABLE 1 test strains and culture media
Note a: ATCC, American Type Culture Collection.
Culture media referred to in the examples of the present invention: MRS liquid medium: 20.0g of glucose, 10.0g of tryptone, 10.0g of beef extract, 5.0g of yeast extract powder, 801.0 m L g of Tween, 2.0g of dipotassium phosphate, 2.0g of ammonium citrate, 5.0g of anhydrous sodium acetate, 0.5g of magnesium sulfate, 0.25g of manganese sulfate monohydrate, 1L of deionized water and pH 6.5 (1.5% agar is added to form a solid culture medium).
BPY medium: 5.0g of beef extract, 5.0g of sodium chloride, 10.0g of peptone, 5.0g of yeast extract powder, 5.0g of glucose, 1L of deionized water and pH 7.3 (1.5% agar is added as a solid culture medium).
LB culture medium: 10.0g of tryptone, 10.0g of sodium chloride, 5.0g of yeast extract powder, 1L of deionized water and 7.3 of pH (1.5% agar is added as a solid culture medium).
Example 1 isolation of Pediococcus pentosaceus SD22 Strain
From home-made pickle samples aseptically collected from great Liangshan in Sichuan, 5g of different samples were put into an aseptic homogeneous bag by a coating flat plate method, marked, and completely flapped and mixed after respectively adding 45mL of 0.85% physiological saline. Then, 100. mu.L of the sample was aspirated to perform 10-fold serial gradient dilutions, each with an aspiration dilution factor of 10-3、10-4、10-5、10-6Sample (5) 100. mu.L coated with 2.5% CaCO3On MRS plate (E), the cells were cultured in an inverted state at 37 ℃ for 24 hours. Colonies with good growth and large calco-lysis circles (as shown in FIG. 1) were picked, and separated and purified repeatedly by plate streaking (as shown in FIG. 2) until a single colony was obtained, and the isolated strain was named SD22, and was deposited in glycerol at-80 ℃.
Example 2 identification of Pediococcus pentosaceus SD22 Strain
(1) Physiological and biochemical test
Gram staining (shown in figure 3) and catalase test are carried out on the screened and purified strain SD22, physiological and biochemical indexes of the strain are measured, and the primary judgment on the strain is carried out according to the test result in the eighth edition of Bergey's Manual of systematic bacteriology. The test shows that the gram stain of the selected strain SD22 is purple and positive. The shape of the cells is spherical, catalase and oxidase are negative, no spore is formed, and the results of physicochemical experiments are shown in Table 2.
Table 2 shows the morphology and physicochemical results of Pediococcus pentosaceus SD22 cells
Note: + is positive, i.e. the substrate can be used for fermentation; negative is indicated.
(2)16S r RNA identification
According to the instruction of the bacterial gene DNA extraction kit, extracting the gene DNA of an unknown strain, and carrying out PCR amplification on the 16S r RNA gene by using the gene DNA as a template. The amplification primers used were universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTT GTTACGACTT-3'). Wherein the PCR reaction system comprises: DNA 1. mu.L, 27F 1. mu.L, 1492R 1. mu.L, Premix Ex Taq 12.5. mu.L, ddH2O9.5. mu.L. And (3) PCR reaction conditions: pre-denaturation at 95 ℃ for 5 min; denaturation at 95 ℃ for 30s, annealing at 55 ℃ for 30s, extension at 72 ℃ for 30s, and 34 cycles; finally, extension is carried out for 5min at 72 ℃. The PCR amplification product was then sent to DNA sequencing (Shanghai Bioengineering Co., Ltd.). The sequencing sequence result is that approximate sequences are searched in NCBI database by Blast software, the measured sequences are compared with 16S r RNA gene sequences of related species obtained from the gene library, and a phylogenetic tree is constructed by applying Mega7.0 software, and the result is shown in figure 4.
27F：5'-AGAGTTTGATCCTGGCTCAG-3'(SEQ ID NO.1)。
1492R：5'-TACGGCTACCTTGTTACGACTT-3'(SEQ ID NO.2)。
The results of sequencing the 16S r RNA gene are as follows:
GCTATACATGCAAGTCGAACGAACTTCCGTTAATTGATTATGACGT ACTTGTACTGATTGAGATTTTAACACGAAGTGAGTGGCGAACGGGTG AGTAACACGTGGGTAACCTGCCCAGAAGTAGGGGATAACACCTGGAA ACAGATGCTAATACCGTATAACAGAGAAAACCGCATGGTTTTCTTTTA AAAGATGGCTCTGCTATCACTTCTGGATGGACCCGCGGCGTATTAGCT AGTTGGTGAGGTAAAGGCTCACCAAGGCAGTGATACGTAGCCGACCT GAGAGGGTAATCGGCCACATTGGGACTGAGACACGGCCCAGACTCCT ACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT GGAGCAACGCCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAGCTCT GTTGTTAAAGAAGAACGTGGGTAAGAGTAACTGTTTACCCAGTGACG GTATTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGT AATACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAG CGCAGGCGGTCTTTTaAGTCTAATGTGAAAGCCTTCGGCTCAACCGAA GAAGTGCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGACAGTG GAACTCCATGTGTAGCGGTGAAAaTGCGTAGATATATGGAAGAACACC AGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAA AGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAA ACGATGATTACTAAGTGTTGGAGGGTTTCCGCCCTTCAGTGCTGCAGC TAACGCATTAAGTAATCCGCCTGGGGAGTACGACCGCAAGGTTGAAA CTCAAAAGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTT TAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATCTTCTGA CAGTCTAAGAGATTAGAGGTTCCCTTCGGGGACAGAATGACAGGTGG TGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCG CAACGAGCGCAACCCTTATTACTAGTTGCCAGCATTAAGTTGGGCACT CTAGTGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGT CAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGG ATGGTACAACGAGTCGCGAGACCGCGAGGTTAAGCTAATCTCTTAAA AACCATTCTCAGTTCGGACTGTAGGCTGCAACTCGCCTACACAAAGTC GGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCC GGGCCTTTGTACACACCGCCCGTCAC(SEQ ID NO.3)。
(3) pheS gene sequence identification
The pheS gene sequence can be used for identifying the lactic acid bacteria, and the pheS gene sequence is used as a primer for amplification to identify the strain.
And (3) extracting the gene DNA of the unknown strain according to the instruction of the bacterial gene DNA extraction kit, and performing PCR amplification of the pheS gene by using the gene DNA as a template. The amplification primers used were the lactic acid bacteria universal primers F (5'-CAYCCNGCHCGYGAYATGC-3') and R (5 '-CCWARVCCRAARGCAAARCC-3'). Wherein the PCR reaction system comprises: DNA 1. mu.L, 27F 1. mu.L, 1492R 1. mu.L, Premix Ex Taq 12.5. mu.L, ddH2O 9.5.5. mu.L. And (3) PCR reaction conditions: pre-denaturation at 95 ℃ for 5 min; denaturation at 95 ℃ for 30s, annealing at 55 ℃ for 30s, extension at 72 ℃ for 30s, and 35 cycles; finally, extension is carried out for 5min at 72 ℃. The PCR amplification product was then sent to DNA sequencing (Shanghai Bioengineering Co., Ltd.). The sequencing sequence result was searched for an approximate sequence in the NCBI database using Blast software, and the measured sequence was compared with the pheS gene sequence of the related species obtained from the gene bank.
The sequences of the primers used for amplification were as follows:
F：5'-CAYCCNGCHCGYGAYATGC-3'(SEQ ID NO.4)。
R：5'-CCWARVCCRAARGCAAARCC-3'(SEQ ID NO.5)。
the pheS gene sequence determination results are as follows:
GGGGGGTTTCGTGTCAACCAGCCATTTTCAAACATTAGGATGAAC CATTCCAGCCCCTAATACTTCTATCCAACCGGTTGACTTACATACTGAA CAGCCTTTTCCCATACAATTAAAGCAAGTAACGTCTGCTTCAACAGAA GGTTCTGTAAATGGAAAATAACTTGGACGCAAGCGTACTTCAAAACG ATCACCAAAAAGTTCCTTAGCCAAAGTTACTAAAGTACCTTTCAAATC ACCCATCGTAATGTTCTTATCAATCACTAATCCTTCAATTTGATGGAATT GGTGTGAATGAGTAGCATCATCAGTATCGCGACGGTAAACTACCCCTG GTGAGATCATTTTTAATGGTCCCTTTGAAAAGTCATGTTTTTCCATTGT TCGAGCTTGAACTGGAGAAGTTTGAGAGCGCATCAAAACTTCGTCCG TGATGTAAAATGTATCTTGCATATCACGAGCAGGATGA(SEQ ID NO.6)。
according to the comprehensive analysis of the cell morphology, physiological and biochemical characteristics, 16S r RNA gene sequence, pheS gene sequence and other data of the strain, the strain is identified as Pediococcus pentosaceus (Pediococcus pentosaceus) SD22 by referring to Boger' S Manual of systematic bacteriology eighth edition.
Example 3 determination of the growth Curve of Pediococcus pentosaceus SD22
(1) Preparation of fermentation broth
Inoculating Pediococcus pentosaceus SD22 preserved at-80 deg.C to MRS liquid culture medium, culturing at 37 deg.C for 24 hr, and continuously transferring for 2 times to complete activation of strain. Then inoculating the activated pediococcus pentosaceus SD22 in 200mL of MRS liquid culture medium in a volume of 2%, uniformly oscillating, culturing at 37 ℃ for 24h, and centrifuging at 4 ℃ and 10000 r/min for 15min to obtain fermentation liquor.
(2) Determination of growth curves
Inoculating activated 1 generation Pediococcus pentosaceus SD22 into 200mL MRS liquid culture medium at 2% volume, shaking uniformly,culturing at 37 deg.C with shaking table at 180r/min, sampling every 1h, and determining OD600nmThe growth curve of Pediococcus pentosaceus SD22 was plotted, as shown in FIG. 5. From FIG. 5, Pediococcus pentosaceus SD22 entered stationary phase starting from around 20 h.
Example 4 bacteriostatic study of Pediococcus pentosaceus SD22 on Streptococcus mutans
Adopting Oxford cup method, respectively taking 100 μ L viable count as 107Placing CFU/mL salmonella and escherichia coli in a plate, pouring a proper amount of heated and melted BPY solid culture medium and LB solid culture medium, uniformly shaking, after cooling and solidifying, sequentially placing oxford cups in the plate at proper intervals, then adding 100 mu L of pediococcus pentosaceus SD22 fermentation liquor into the holes of the oxford cups, taking MRS liquid culture medium as negative control, taking nisin as positive control, and repeating for 3 times. After incubation for 24h at 37 ℃, photographs were taken and the zone diameter was measured with a vernier caliper by the cross method, and the results are shown in fig. 6 and 7.
The test result shows that the diameter of the inhibition zone of the pediococcus pentosaceus SD22 on the salmonella is (16.25 +/-0.98) mm, which is slightly higher than that of a positive control Nisin (15.48 +/-0.26) mm; the diameter of the inhibition zone for the escherichia coli is (16.41 +/-0.12) mm, which is higher than that of a positive control Nisin (12.24 +/-0.32) mm. The result shows that the pediococcus pentosaceus SD22 has a good inhibition effect on both salmonella and escherichia coli.
In conclusion, the pediococcus pentosaceus SD22 provided by the invention is separated and screened from sauerkraut made by peasants in great summer mountains in Sichuan, has high safety, has an obvious inhibiting effect on salmonella and escherichia coli, is beneficial to reducing the harm of food-borne diseases caused by the salmonella and the escherichia coli to human health, and has high industrial application value.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
SEQUENCE LISTING
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Claims (10)
1. The pediococcus pentosaceus SD22 is characterized in that the pediococcus pentosaceus SD22 is preserved in China general microbiological culture Collection center (CGMCC), and the preservation numbers are as follows: CGMCC No. 19327.
2. The screening method for Pediococcus pentosaceus SD22 according to claim 1, comprising the steps of:
separating and purifying the strain to obtain a single colony, and then identifying the single colony to obtain pediococcus pentosaceus SD 22;
the strain is sampled from agricultural pickled vegetables in big summer mountains in Sichuan.
3. The screening method for Pediococcus pentosaceus SD22 according to claim 2, wherein the separation and purification method comprises a spread plate method and a plate drawing method.
4. The screening method for Pediococcus pentosaceus SD22 according to claim 2, wherein the identification method comprises physiological and biochemical tests, 16Sr RNA gene sequence analysis, and pheS gene sequence analysis.
5. A microbial preparation comprising the pediococcus pentosaceus SD22 according to claim 1.
6. The microbial inoculum according to claim 5, wherein the formulation of the microbial inoculum comprises liquid microbial inoculum, powder and granular microbial inoculum.
7. A fermentation product obtained by fermentation with the microorganism of Pediococcus pentosaceus SD22 of claim 1 or 5 or 6.
8. Use of pediococcus pentosaceus SD22 of claim 1, the microbial inoculum of claim 5 or 6 or the fermentation product of claim 7 for the preparation of a product for controlling food-borne pathogenic bacteria.
9. The use of claim 8, wherein the food-borne pathogenic bacteria comprise salmonella and escherichia coli.
10. A product for controlling food-borne pathogenic bacteria, which comprises Pediococcus pentosaceus SD22 of claim 1, the microbial inoculum of claim 5 or 6, or the fermentation product of claim 7.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005218324A (en) * | 2004-02-04 | 2005-08-18 | Eromlife Co Ltd | Pediococcus pentosaceus erom101, its mutant or variant, immunopotentiating composition, anticancer composition, antimicrobial composition, composition for controlling intestinal function, composition for live bacterium agent, composition for feed, composition for food addition and fermented product containing them, method for culturing them and method for controlling microbial growth |
| CN111793586A (en) * | 2020-08-13 | 2020-10-20 | 石河子大学 | Breast milk source pediococcus pentosaceus and application thereof |
| CN113151064A (en) * | 2021-03-25 | 2021-07-23 | 中国科学院合肥物质科学研究院 | Pediococcus pentosaceus and application thereof |
| CN113388551A (en) * | 2021-07-16 | 2021-09-14 | 新希望六和股份有限公司 | Pediococcus pentosaceus NHB-PpA9601 and application thereof |
| CN113528381A (en) * | 2021-06-30 | 2021-10-22 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | An antagonistic escherichia coli O157: h7 pediococcus pentosaceus and application thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005218324A (en) * | 2004-02-04 | 2005-08-18 | Eromlife Co Ltd | Pediococcus pentosaceus erom101, its mutant or variant, immunopotentiating composition, anticancer composition, antimicrobial composition, composition for controlling intestinal function, composition for live bacterium agent, composition for feed, composition for food addition and fermented product containing them, method for culturing them and method for controlling microbial growth |
| CN111793586A (en) * | 2020-08-13 | 2020-10-20 | 石河子大学 | Breast milk source pediococcus pentosaceus and application thereof |
| CN113151064A (en) * | 2021-03-25 | 2021-07-23 | 中国科学院合肥物质科学研究院 | Pediococcus pentosaceus and application thereof |
| CN113528381A (en) * | 2021-06-30 | 2021-10-22 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | An antagonistic escherichia coli O157: h7 pediococcus pentosaceus and application thereof |
| CN113388551A (en) * | 2021-07-16 | 2021-09-14 | 新希望六和股份有限公司 | Pediococcus pentosaceus NHB-PpA9601 and application thereof |
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