CN114292730A

CN114292730A - Kit for screening dilated cardiomyopathy hereditary genes

Info

Publication number: CN114292730A
Application number: CN202210022195.4A
Authority: CN
Inventors: 刘琳; 唐颢; 邢珺月
Original assignee: Fuwai Central China Cardiovascular Hospital
Current assignee: Fuwai Central China Cardiovascular Hospital
Priority date: 2022-01-10
Filing date: 2022-01-10
Publication date: 2022-04-08

Abstract

The invention discloses an expanded cardiomyopathy hereditary gene screening kit which comprises a kit antifouling outer box with an opening at one end, wherein a detachable gene reaction tube is arranged in the kit antifouling outer box, and an auxiliary reactant quantitative adding tube capable of quantitatively discharging liquid is arranged in the gene reaction tube. Establish the inside at the antifouling outer box of kit with gene reaction pipe box, can guarantee the outside cleanliness of gene reaction pipe, prevent to operate the surface adhesion dust or other miscellaneous dirt of practical process gene reaction pipe, the antifouling outer box of kit is flat cylinder structure simultaneously, the putting of the gene reaction pipe of being convenient for. Meanwhile, the auxiliary reactant quantitative adding pipe is arranged inside the gene reaction pipe, and the quantitative addition of the general solution is realized in an internal sealing state, so that the cleanness of the inside of the gene reaction pipe is guaranteed, and meanwhile, the general solution is prevented from overflowing.

Description

Kit for screening dilated cardiomyopathy hereditary genes

Technical Field

The invention relates to the field of dilated cardiomyopathy screening, in particular to a dilated cardiomyopathy hereditary gene screening kit.

Background

Dilated Cardiomyopathy (DCM) is a primary cardiomyopathy of unknown cause. The disease is characterized by enlargement of the left or right ventricle or both ventricles, with reduced ventricular contractile function, with or without congestive heart failure. Ventricular or atrial arrhythmias are common. The disease becomes progressively worse and death may occur at any stage of the disease. A recently published european cardiomyopathy registration study showed that approximately 40% of cardiomyopathy patients have a family history, in other words, that their cardiomyopathy is genetic cardiomyopathy. Therefore, researchers believe that it is of great importance to the relatives screening for cardiomyopathies in patients with cardiomyopathies. The relatives with related gene mutation can be discovered as soon as possible, so that the relatives can be better treated and complications including sudden death can be prevented, and the genetic detection can be carried out on patients with the cardiomyopathy and the relatives with the gene mutation can be subjected to the genetic screening to quickly detect whether the relatives are inherited with the cardiomyopathy or not. In the traditional gene screening, a sample of a person to be tested is placed in a gene reaction tube, then three times of volume of general solution is added through a dropper, the solution is dissolved in water at 65 ℃ for 5-10 minutes, the solution is transferred to a centrifugal column, the centrifugation is carried out for half a minute, general column washing liquid is added, and the centrifugation is carried out for half a minute. Repeat the washing step once: centrifuging for half a minute, spin-drying the residual liquid, placing the centrifugal column in a new centrifugal tube, adding the eluent, and centrifuging for half a minute to obtain the sample in the centrifugal tube. The disposable quick quantitative adding of the general solution can not be realized in the adding process by filling the general solution through the dropper, the frequent reciprocating adding is needed, the dropper easily causes the general solution to drip in the moving process, meanwhile, when the adding is carried out, the gene reaction tube needs to be held by one hand, the dropper is held by the other hand, if the adding is carried out manually, the dropper easily drips to the outside or fingers, further, the cross contamination of the operation environment is caused, meanwhile, in the adding process of the solution, the opening of the kit is always in an open state, therefore, the environmental requirement on the screening reaction is higher, and the phenomenon that the external dust enters the inside of the gene reaction tube is avoided

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides the kit for screening the hereditary genes of the dilated cardiomyopathy, which can realize the quantitative addition of the general solution in a sealed state through the arranged auxiliary reagent quantitative addition pipe, has relatively low requirement on the cleanness degree of the experimental environment, avoids the general solution from being scattered and the pollution to the operation environment in the operation process, and effectively avoids the internal pollution of the genetic reaction pipe in a sealed state.

In order to solve the technical problems, the invention provides the following technical scheme: an expanded cardiomyopathy hereditary gene screening kit comprises a kit antifouling outer box with an opening at one end, wherein a gene reaction tube is detachably arranged in the kit antifouling outer box, and an auxiliary reactant quantitative adding tube capable of quantitatively discharging liquid is arranged in the gene reaction tube;

the auxiliary reactant quantitative adding pipe comprises an auxiliary pipe cover detachably connected with the top opening end of the gene reaction pipe, and an auxiliary storage pipe which is arranged in the gene reaction pipe and provided with a liquid outlet at the bottom, a liquid storage cavity and a quantitative storage cavity are arranged in the auxiliary storage pipe, and a flow guide pipe fitting penetrating through the liquid storage cavity and the quantitative storage cavity is axially and movably arranged in the auxiliary storage pipe;

when the lower end of the flow guide pipe fitting is attached to the liquid outlet, the liquid outlet is in a sealed state, and the liquid storage cavity and the quantitative storage cavity are communicated with each other;

when the lower extreme of water conservancy diversion pipe fitting breaks away from with the liquid outlet, the liquid outlet is in the open mode, and liquid storage chamber, quantitative storage chamber are isolated each other.

In a preferred embodiment of the present invention, a partition is fixedly disposed in the auxiliary storage tube, and the quantitative storage chamber and the liquid storage chamber are separated by the partition. .

In a preferred embodiment of the present invention, the inner wall of the auxiliary tube cap is formed with an inner thread groove which is in threaded engagement with the outer wall of the open end of the gene reaction tube, and the edge of the auxiliary tube cap is formed with an embedded thread groove which is in threaded engagement with the open end of the antifouling box for a kit.

As a preferred technical scheme of the present invention, the flow guiding pipe is coaxially disposed with the auxiliary tube cap and the partition plate, and one end of the flow guiding pipe, which is far away from the liquid outlet, is elastically connected with the auxiliary tube cap.

As a preferred technical scheme, the flow guide pipe fitting comprises a solid rod arranged in a liquid storage cavity and a hollow pipe fixedly connected to the bottom of the solid rod and arranged in a quantitative storage cavity, a sealing ball matched with a liquid outlet is mounted at one end, far away from the solid rod, of the hollow pipe, a plurality of liquid inlet holes are annularly formed at one end, close to the solid rod, of the hollow pipe, the liquid inlet holes can be movably switched between the liquid storage cavity and the quantitative storage cavity, and a plurality of liquid outlet holes are annularly formed at one end, close to the sealing ball, of the hollow pipe.

In a preferred embodiment of the present invention, a pressing plate located outside the auxiliary tube cover is fixedly connected to one end of the solid rod away from the sealing ball, and a spring is disposed between the pressing plate and the auxiliary tube cover.

In a preferred embodiment of the present invention, an outer surface of the auxiliary tube cap is provided with an external threaded tube higher than the pressing plate, and a protective cap is screwed to an outer side of the external threaded tube.

In a preferred embodiment of the present invention, the auxiliary tube cover is provided with a movable hole matching with the flow guiding tube and a liquid injection hole located at one side of the movable hole, the movable hole and the liquid injection hole are both located at the inner side of the external threaded tube, and a tower-shaped plug with a tower-shaped structure is installed inside the liquid injection hole.

In a preferred embodiment of the present invention, a plurality of rubber seal rings are provided inside the liquid inlet.

In a preferred embodiment of the present invention, the anti-fouling outer box, the gene reaction tube and the auxiliary reagent quantitative adding tube are made of transparent PVC, and the open end of the gene reaction tube is connected with a cap by a screw thread.

Compared with the prior art, the invention can achieve the following beneficial effects:

1. the quantitative addition of general solution can be realized under sealing state through the supplementary reactant ration interpolation pipe that sets up, and experimental environment's clean degree requirement is low relatively, and simultaneously at the in-process of operation, it is unrestrained to avoid general solution, avoids operation environment's pollution, and simultaneously under the sealing state, the inside pollution of effectual avoidance gene reaction pipe.

2. Through the visor that sets up, through the threaded connection of visor and external screw thread pipe, the axial height of external screw thread pipe is higher than the pressure dish for press the pressure dish when not using, avoid the mistake to touch and press the pressure dish.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic illustration of an explosive structure according to the present invention;

FIG. 3 is a schematic structural view of a liquid outlet and a sealing ball of the present invention in a closed state;

FIG. 4 is an enlarged view of the structure at A in FIG. 3 according to the present invention;

FIG. 5 is an enlarged view of the structure of FIG. 3B according to the present invention;

FIG. 6 is an enlarged view of the structure of FIG. 3 at C according to the present invention;

FIG. 7 is a schematic structural view of the liquid outlet and the sealing ball of the present invention in an open state;

FIG. 8 is an enlarged view of the structure of FIG. 7 at D in accordance with the present invention;

FIG. 9 is an enlarged view of E of FIG. 7 according to the present invention;

FIG. 10 is a schematic view of the structure of the flow guiding tube and the sealing ball of the present invention;

FIG. 11 is a cross-sectional view of a flow tube and a sealing ball according to the present invention;

FIG. 12 is a schematic sectional view of an auxiliary storage tube according to the present invention;

FIG. 13 is an enlarged view of the structure of FIG. 12 at F in accordance with the present invention;

FIG. 14 is a schematic view of a tower plug configuration of the present invention;

FIG. 15 is a schematic view showing a structure of installing a gene reaction tube and a cap according to the present invention;

FIG. 16 is a schematic view of the antifouling outer box of the kit of the present invention.

Wherein:

1. the kit is an anti-fouling outer box;

2. a gene reaction tube; 21. a cover;

3. an auxiliary reactant quantitative addition pipe;

31. an auxiliary agent storage tube; 311. a liquid outlet;

32. a partition plate; 321. a liquid storage cavity; 322. a quantitative storage chamber;

33. an auxiliary agent pipe cover; 331. an internal thread groove; 332. an embedded thread groove; 333. a movable hole; 334. a liquid injection hole; 335. a tower plug; 336. a rubber seal ring; 337. an externally threaded tube;

34. a flow directing pipe fitting; 341. a solid rod; 342. a hollow tube; 3421. a liquid inlet hole; 3422. a liquid outlet hole; 343. a sealing ball; 344. pressing the disc;

35. a spring; 36. a protective cover.

Detailed Description

The present invention will be further described with reference to specific embodiments for the purpose of facilitating an understanding of technical means, characteristics of creation, objectives and functions realized by the present invention, but the following embodiments are only preferred embodiments of the present invention, and are not intended to be exhaustive. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example (b):

as shown in figures 1-16, the invention provides an expanded cardiomyopathy hereditary gene screening kit, which comprises a kit anti-fouling outer box 1 with an opening at one end, wherein a gene reaction tube 2 is detachably arranged in the kit anti-fouling outer box 1, and an auxiliary reactant quantitative adding tube 3 capable of quantitatively outputting liquid is arranged in the gene reaction tube 2. Establish the gene reaction tube 2 cover in the antifouling outer box 1's of kit inside, can guarantee the outside cleanliness of gene reaction tube 2, prevent to operate practical process gene reaction tube 2's surface adhesion dust or other miscellaneous dirt, the antifouling outer box 1 of kit is flat cylinder structure simultaneously, the putting of gene reaction tube 2 of being convenient for (as shown in figure 1). Meanwhile, the auxiliary reagent quantitative adding pipe 3 is arranged inside the gene reaction pipe 2, and the quantitative addition of the general solution is realized in an internal sealing state, so that the cleanness inside the gene reaction pipe 2 is guaranteed, and meanwhile, the general solution is prevented from overflowing (as shown in fig. 3).

The auxiliary reagent quantitative adding tube 3 comprises an auxiliary tube cap 33 detachably connected with the open end of the top of the gene reaction tube 2, and an auxiliary storage tube 31 arranged inside the gene reaction tube 2 and provided with a liquid outlet 311 at the bottom. The inner wall of the auxiliary tube cover 33 is provided with an internal thread groove 331 which is matched with the external thread on the outer wall of the open end of the gene reaction tube 2, the edge of the bottom surface of the auxiliary tube cover 33 is provided with an embedded thread groove 332, when the auxiliary tube cover 33 is screwed on the top of the gene reaction tube 2 through thread connection, the embedded thread groove 332 is synchronously in thread connection with the thread arranged at the withdrawing position of the open end of the reagent kit anti-fouling outer box 1, so that the gene reaction tube 2 is conveniently fixed inside the reagent kit anti-fouling outer box 1 (as shown in figures 3 and 12).

A liquid storage cavity 321 and a quantitative storage cavity 322 are arranged in the auxiliary storage tube 31, and a flow guide tube 34 penetrating through the liquid storage cavity 321 and the quantitative storage cavity 322 is axially and movably arranged in the auxiliary storage tube 31. A partition plate 32 is provided in the auxiliary storage tube 31, and the partition plate 32 is fixedly and hermetically connected to the inner wall of the auxiliary storage tube 31, so that the quantitative storage chamber 322 and the reservoir 321 are partitioned by the partition plate 32.

The flow guiding pipe member 34 is coaxially disposed with the auxiliary pipe cap 33 and the partition 32, and one end of the flow guiding pipe member 34 away from the liquid outlet 311 is elastically connected with the auxiliary pipe cap 33. The fluid guiding tube 34 includes a solid rod 341 disposed inside the fluid storage cavity 321, and a hollow tube 342 fixedly connected to the bottom of the solid rod 341 and disposed inside the quantitative storage cavity 322. The sealing ball 343 adapted to the liquid outlet 311 is installed at one end of the hollow tube 342 far away from the solid rod 341, a plurality of liquid inlet holes 3421 are annularly formed at one end of the hollow tube 342 near the solid rod 341, the liquid inlet holes 3421 can be moved in the liquid storage cavity 321 and the quantitative storage cavity 322, and a plurality of liquid outlet holes 3422 are annularly formed at one end of the hollow tube 342 near the sealing ball 343.

The hollow tube 342 and the solid rod 341 are integrally formed, when the liquid inlet hole 3421 is disposed at one side of the liquid storage cavity 321 (as shown in fig. 5), the liquid storage cavity 321 is communicated with the quantitative storage cavity 322, and the sealing ball 343 is sealed against the liquid outlet 311 (as shown in fig. 6), the general solution in the liquid storage cavity 321 supplements the solution in the quantitative storage cavity 322 through the liquid outlet hole 3422; when the liquid inlet 3421 is located inside the quantitative storage chamber 322 (as shown in fig. 8), the liquid storage chamber 321 and the quantitative storage chamber 322 are closed, the sealing ball 343 is separated from the liquid outlet 311 (as shown in fig. 9), and the quantitative solution in the quantitative storage chamber 322 is discharged through the liquid outlet 311.

Preferably, a pressing plate 344 located outside the auxiliary tube cover 33 is fixedly coupled to an end of the solid rod 341 remote from the sealing ball 343, and a spring 35 (shown in fig. 4) is provided between the pressing plate 344 and the auxiliary tube cover 33. Under the elastic supporting action of the spring 35, the sealing ball 343 is in a fitting and sealing state with the liquid outlet 311 in a normal state; the pressing plate 344 is provided to facilitate the pressing operation of the guiding pipe 34, i.e. the separation operation of the sealing ball 343 from the liquid outlet 311.

Preferably, the outer surface of the auxiliary tube cover 33 is provided with an external threaded tube 337 higher than the pressing plate 344, and the external threaded tube 337 is screw-coupled with the protective cover 36 such that the external threaded tube 337 is higher in axial height than the pressing plate 344 when the pressing plate 344 is not in use, thereby preventing erroneous contact with the pressing plate 344. The auxiliary pipe cover 33 is provided with a movable hole 333 which is axially matched with the flow guide pipe member 34 in a sliding way and a liquid injection hole 334 which is positioned at one side of the movable hole 333, the movable hole 333 and the liquid injection hole 334 are both positioned at the inner side of the external thread pipe 377, and a tower-shaped plug 335 with a tower-shaped structure is arranged in the liquid injection hole 334. After the tower plug 335 is removed, the common solution can be filled into the auxiliary storage tube 31 through the filling hole 334 (see fig. 4).

General solution is stored in the liquid storage cavity 321, the liquid storage cavity 321 and the quantitative storage cavity 322 are communicated with each other through the flow guide pipe fitting 34, the liquid storage cavity 321 can supplement the general solution to the inside of the quantitative storage cavity 322 (as shown in fig. 3 and 5), and the general solution in the quantitative storage cavity 322 can be added to the inside of the gene reaction tube 2 through the liquid outlet 311 at the bottom. When the sealing ball 343 at the lower end of the guiding tube 34 is engaged with the liquid outlet 311, the liquid outlet 311 is in a sealed state, that is, the quantitative storage cavity 322 is in a sealed state, and meanwhile, the quantitative storage cavity 322 is communicated with the liquid storage cavity 321 through the liquid inlet 3421 (as shown in fig. 3-6), so that the liquid in the liquid storage cavity 321 flows into the quantitative storage cavity 322 through the liquid inlet 3421, and the quantitative storage cavity 322 is filled with the liquid. When the sealing ball 343 at the lower end of the flow guide tube 34 is separated from the liquid outlet 311, the liquid outlet 311 is in an open state, and the liquid storage cavity 321 and the quantitative storage cavity 322 are sealed with each other, so that the solution with a fixed volume stored in the quantitative storage cavity 322 falls into the gene reaction tube 2, thereby realizing quantitative transportation of the general solution (as shown in fig. 7 to 9).

Preferably, the auxiliary agent pipe cover 33 is of a thickened type, and a plurality of rubber seal rings 336 are provided inside the pouring hole 334. The antifouling outer box 1, the gene reaction tube 2 and the auxiliary reactant quantitative adding tube 3 of the kit are all made of transparent PVC materials, and the opening end of the gene reaction tube 2 is in threaded connection with a cover 21. Through the laminating between rubber seal ring 336 and the water conservancy diversion pipe fitting 34, improve the leakproofness (as shown in fig. 13) between water conservancy diversion pipe fitting 34 and the stock solution chamber 321, the antifouling outer box 1 of kit, gene reaction pipe 2, the preferred transparent PVC material of supplementary reactant ration interpolation pipe 3 simultaneously, be convenient for observe inside reaction condition to when adding the back, through lid 21 with gene reaction pipe 2 sealed (as shown in fig. 15), place the reactor in and carry out operation on next step.

When the quantitative storage device is used, in a normal state, under the elastic supporting action of the spring 35, the sealing ball 343 and the liquid outlet 311 are in a sealing state, the liquid inlet hole 3421 is arranged on one side of the liquid storage cavity 321, the liquid storage cavity 321 is communicated with the quantitative storage cavity 322, so that the general solution in the liquid storage cavity 321 is filled into the quantitative storage cavity 322 through the liquid inlet hole 3421 and the liquid outlet hole 3422, then the pressure plate 344 is pressed by fingers, the liquid inlet hole 3421 is arranged in the quantitative storage cavity 322, at the moment, the liquid storage cavity 321 is closed with the quantitative storage cavity 322, the sealing ball 343 is separated from the liquid outlet 311, the quantitative general solution in the quantitative storage cavity 322 is discharged through the liquid outlet 311 and falls into the gene reaction tube 2;

after the completion of the addition, press disc 344 to loosen, this moment under the effect of spring 35 elasticity resilience, make outside jack-up of water conservancy diversion pipe fitting 34, ball sealer 343 offsets with liquid outlet 311, the realization is to the sealed of ration storage chamber 322, stock solution chamber 321 one side is arranged in to feed liquor hole 3421 simultaneously, stock solution chamber 321 communicates with ration storage chamber 322 each other, the inside general solution of stock solution chamber 321 passes through feed liquor hole 3421 and stores the inside solution that is full of chamber 322 to the ration, realize the ration of general solution and add, experimental environment's clean degree requires to be low relatively, simultaneously at the in-process of operation, avoid general solution unrestrained, avoid operating environment's pollution, simultaneously under the encapsulated situation, 2 internal contamination of effectual avoidance gene reaction pipe.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. An expansive cardiomyopathy hereditary gene screening kit is characterized in that: the kit anti-fouling outer box comprises a kit anti-fouling outer box (1) with an opening at one end, wherein a gene reaction tube (2) is detachably arranged in the kit anti-fouling outer box (1), and an auxiliary reactant quantitative adding tube (3) capable of quantitatively discharging liquid is arranged in the gene reaction tube (2);

the auxiliary reactant quantitative adding pipe (3) comprises an auxiliary pipe cover (33) detachably connected with the open end of the top of the gene reaction pipe (2) and an auxiliary storage pipe (31) which is arranged in the gene reaction pipe (2) and provided with a liquid outlet (311) at the bottom, a liquid storage cavity (321) and a quantitative storage cavity (322) are arranged in the auxiliary storage pipe (31), and a flow guide pipe fitting (34) penetrating through the liquid storage cavity (321) and the quantitative storage cavity (322) is axially and movably arranged in the auxiliary storage pipe (31);

when the lower end of the flow guide pipe fitting (34) is attached to the liquid outlet (311), the liquid outlet (311) is in a sealed state, and the liquid storage cavity (321) and the quantitative storage cavity (322) are communicated with each other;

when the lower end of the flow guide pipe fitting (34) is separated from the liquid outlet (311), the liquid outlet (311) is in an open state, and the liquid storage cavity (321) and the quantitative storage cavity (322) are isolated from each other.

2. The kit for screening dilated cardiomyopathy inherited genes, according to claim 1, wherein the kit comprises: a partition plate (32) is fixedly arranged in the auxiliary storage tube (31), and the quantitative storage cavity (322) and the liquid storage cavity (321) are separated by the partition plate (32).

3. The kit for screening dilated cardiomyopathy inherited genes, according to claim 1, wherein the kit comprises: the inner wall of the auxiliary pipe cover (33) is provided with an inner thread groove (331) which is in thread fit with the outer wall of the opening end of the gene reaction pipe (2), the edge of the auxiliary pipe cover (33) is provided with an embedded thread groove (332), and the embedded thread groove (332) is in thread connection with the opening end of the antifouling kit outer box (1).

4. The kit for screening dilated cardiomyopathy inherited genes, according to claim 1, wherein the kit comprises: the flow guide pipe fitting (34), the auxiliary pipe cover (33) and the partition plate (32) are coaxially arranged, and one end, far away from the liquid outlet (311), of the flow guide pipe fitting (34) is elastically connected with the auxiliary pipe cover (33).

5. The kit for screening dilated cardiomyopathy inherited genes, according to any one of claims 1 to 4, wherein the kit comprises: shown water conservancy diversion pipe fitting (34) are including setting up in the inside solid pole (341) of stock solution chamber (321), fixed connection in the bottom of solid pole (341) and set up in the inside hollow tube (342) of ration storage chamber (322), the hollow tube (342) are kept away from the one end of solid pole (341) and are installed ball sealer (343) with liquid outlet (311) adaptation, hollow tube (342) are close to solid pole (341) one end annular and have seted up a plurality of feed liquor hole (3421), feed liquor hole (3421) can be in the inside removal switching of stock solution chamber (321) and ration storage chamber (322), hollow tube (342) are close to ball sealer (343) one end annular and have seted up a plurality of liquid holes (3422).

6. The kit for screening dilated cardiomyopathy inherited genes, according to claim 5, wherein the kit comprises: one end of the solid rod (341) far away from the sealing ball (343) is fixedly connected with a pressing disc (344) positioned on the outer side of the auxiliary pipe cover (33), and a spring (35) is arranged between the pressing disc (344) and the auxiliary pipe cover (33).

7. The kit for screening dilated cardiomyopathy inherited genes, according to claim 6, wherein the kit comprises: the outer surface of the auxiliary pipe cover (33) is provided with an external thread pipe (337) higher than the pressing disc (344), and the outer side of the external thread pipe (337) is in threaded connection with a protective cover (36).

8. The kit for screening dilated cardiomyopathy inherited genes, according to claim 7, wherein the kit comprises: the auxiliary pipe cover (33) is provided with a movable hole (333) matched with the flow guide pipe (34) and a liquid injection hole (334) positioned on one side of the movable hole (333), the movable hole (333) and the liquid injection hole (334) are both positioned on the inner side of the external threaded pipe (377), and a tower-shaped plug (335) with a tower-shaped structure is arranged in the liquid injection hole (334).

9. The kit for screening dilated cardiomyopathy inherited genes, according to claim 8, wherein the kit comprises: and a plurality of rubber sealing rings (336) are arranged in the liquid injection hole (334).

10. The kit for screening dilated cardiomyopathy inherited genes, according to claim 1, wherein the kit comprises: the kit is characterized in that the antifouling outer box (1), the gene reaction tube (2) and the auxiliary reactant quantitative adding tube (3) are all made of transparent PVC materials, and the opening end of the gene reaction tube (2) is in threaded connection with a cover (21).