CN114292639B - Multifunctional nano material based on aggregation-induced emission and MXenes, preparation method and application - Google Patents
Multifunctional nano material based on aggregation-induced emission and MXenes, preparation method and application Download PDFInfo
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Abstract
The invention discloses a multifunctional nanomaterial based on aggregation-induced emission and MXenes, a preparation method and application, wherein the method comprises the following steps: dispersing TBFT, rare earth up-conversion luminescent nano material and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-amino cross-linked substance (DSPE-PEG 2000-NH 2) in an organic solvent, and sequentially carrying out ultrasonic and dialysis treatment to obtain nanospheres with amino groups on the surfaces; adding the nanospheres with amino groups on the surface to a titanium carbide nanosheet (Ti 3 C 2 NSs) to obtain multifunctional nanomaterials (TUT NPs) based on aggregation-induced emission and mxnes. Rare earth up-conversion luminescent nano-material and TBFT molecules are wrapped in the inner space of the polymer through amphiphilic polymer DSPE-PEG2000-NH 2. And conventional use of polymers with Ti 3 C 2 Static electricity between NSsCovalent bonding of the polymer surface allows TUT NPs to have long-term stability and efficient tumor accumulation compared to strategies with surface modification.
Description
Technical Field
The invention belongs to the technical field of fluorescent nano materials, and particularly relates to a multifunctional nano material based on aggregation-induced emission and MXenes, a preparation method and application.
Background
MXenes are generally produced by forming a laminate from M n+1 AX n Prepared by extraction of element A in bulk phase, wherein M represents early transition metal carbide, A represents group 13 or 14 element, X represents C or N, e.g. Ti 3 C 2 Nanoplatelets (nanoplatelets are typically MXenes). Ti (Ti) 3 C 2 The nano-sheet has great application potential in the field of photo-thermal treatment (PTT) due to the various surface chemical characteristics, excellent conductivity, sufficient surface end groups and high photo-thermal conversion efficiency (PCE). Ti (Ti) 3 C 2 The nanoplatelets have a higher absorption capacity in the near infrared one region (NIR-I, 750-1000 nm), which favors deep tissue penetration. Ti (Ti) 3 C 2 The photo-thermal conversion efficiency (PCE) of up to 40% is even higher than many reported PTT Photosensitizers (PSs), such as gold nanorods, carbon-based nanomaterials and many organic nanoparticles.
However, ti is 3 C 2 The development of nanoplates in phototherapy is far from adequate. First, ti 3 C 2 The nanoplatelets are non-fluorescent under NIR-I irradiation and therefore do not have FLI navigation capabilities. In addition, mxnes is similar to graphene, and its severe quenching effect makes it inefficient when directly attached to fluorescent probes. Thus, FLI navigates at Ti 3 C 2 The nanometer sheet has important significance in phototherapy, but is still difficult to realize at present. In terms of biocompatibility, traditional surface modification strategies involve polymers with Ti 3 C 2 Electrostatic force interactions between nanoplates, which are too weak in physiological environments, may lead to structural separationAnd to degrade and produce biotoxicity.
Thus, the prior art is still further improved.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a multifunctional nanomaterial based on aggregation-induced emission and MXes, a preparation method and application thereof, and aims to solve the problems that the existing MXes material is weak in stability in physiological environment and does not emit fluorescence in a near infrared region.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a preparation method of a multifunctional nanomaterial based on aggregation-induced emission and MXenes, wherein the method comprises the following steps:
dispersing TBFT, rare earth up-conversion luminescent nano material and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-amino cross-linked substance in an organic solvent, and sequentially carrying out ultrasonic treatment and dialysis treatment to obtain nanospheres with amino groups on the surfaces;
and adding the nanospheres with the amino groups on the surfaces into phosphate buffer salt solution of titanium carbide nano sheets with carboxyl functions to react, so as to obtain the multifunctional nano material based on aggregation-induced emission and MXenes.
Optionally, the preparation method, wherein the rare earth up-conversion luminescence nanomaterial is NaYF 4 :Yb 3+ ，Er 3+ Or NaYF 4 :Yb 3+ ，Tm 3+ 。
Optionally, the preparation method, wherein the organic solvent is selected from one of tetrahydrofuran, acetonitrile and dimethyl sulfoxide.
Optionally, the preparation method comprises the steps of dispersing TBFT, rare earth up-conversion luminescent nano material and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-amino cross-linked substance in an organic solvent, and sequentially carrying out ultrasonic and dialysis treatment to obtain the nanospheres with the amino groups on the surfaces, wherein the nanospheres comprise the following specific steps:
dispersing TBFT, rare earth up-conversion luminescent nano material and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-amino cross-linked substance in an organic solvent, standing, and injecting into deionized water after standing;
carrying out ultrasonic treatment by adopting a microprobe sonar device to obtain a mixture;
transferring the mixture into a dialysis tube, and dialyzing in deionized water to remove the organic solvent, thereby obtaining the nanospheres with amino groups on the surfaces.
Optionally, the preparation method comprises the steps of adding the nanospheres with the amino groups on the surfaces into phosphate buffer salt solution containing titanium carbide nano sheets with carboxyl functions for reaction to obtain the multifunctional nanomaterial based on aggregation-induced emission and MXenes, and specifically comprises the following steps:
stirring phosphate buffer salt solution containing 1-ethyl 3- [ 3-dimethylaminopropyl ] carbodiimide hydrochloride, N-hydroxysuccinimide and titanium carbide nano-sheets with carboxyl functions for 10-30min;
adding nanospheres with amino groups on the surfaces into the phosphate buffer solution, preserving overnight in a shaking table at 37 ℃, and sequentially carrying out centrifugal separation and deionized water washing to obtain the multifunctional nanomaterial based on aggregation-induced emission and MXenes.
Optionally, the preparation method of the titanium carbide nano-sheet with carboxyl function comprises the following steps:
dispersing sodium hydroxide and 4-aminobenzoic acid in water to obtain a benzene carboxylic acid diazonium salt water solution;
maintaining the temperature of the benzene carboxylic acid diazonium salt water solution at 0-5 ℃, and sequentially adding sodium nitrite and hydrochloric acid to obtain a pale yellow solution;
and adding the pale yellow solution into the aqueous solution of the titanium carbide nanosheets, and sequentially stirring, carrying out ultrasonic treatment and centrifugal treatment to obtain the titanium carbide nanosheets with the carboxyl function.
Multifunctional nanomaterial based on aggregation-induced emission and MXenes, wherein the multifunctional nanomaterial is prepared by the preparation method.
The use of the multifunctional nanomaterial based on aggregation-induced emission and mxnes as described above, wherein the multifunctional nanomaterial based on aggregation-induced emission and mxnes is used as a nanosensitive agent in tumor imaging.
The use of the multifunctional nanomaterial based on aggregation-induced emission and mxnes as described above, wherein the multifunctional nanomaterial based on aggregation-induced emission and mxnes is used as an antitumor drug.
The beneficial effects are that: according to the multifunctional nanomaterial based on aggregation-induced emission and MXnes, an amphiphilic polymer distearoyl phosphatidylethanolamine-polyethylene glycol 2000-amino cross-linked substance (DSPE-PEG 2000-NH 2) is used for wrapping rare earth up-conversion luminescent nanomaterial (UCNPs) and TBFT molecules in an inner space of the polymer by a precipitation method, and PEG2000-NH2 is left on the outer surface to form TU NPs. Titanium carbide nanoplates functionalized by-NH 2 and carboxyl groups (Ti 3 C 2 ) Amidation of the surface-COOH, ti 3 C 2 The nanoplatelets are integrated with TU NPs to form multifunctional nanoplatform TUT NPs. Due to TBFT molecules and Ti 3 C 2 The spatial separation between nanoplatelets ensures bright fluorescence of the TBFT, while spatial confinement of TBFT molecules and UCNPs also enables NIR-I regulation of TBFT. And conventional use of polymers with Ti 3 C 2 Compared with the strategy of surface modification by electrostatic force between nano-sheets, covalent bonding of the polymer surface enables TUT NPs to have long-term stability and high-efficiency tumor accumulation. By means of Ti 3 C 2 The photo-thermal conversion capability, FLI navigation capability and ROS generation capability of TBFT, which are indispensable, the PDT/PTT nano platform guided by FLI/PAI/PTI three-mode imaging realizes AIE-PSs and Ti 3 C 2 The win-win cooperation of the nanoplates shows great potential in the efficient diagnosis and treatment of cancer.
Drawings
FIG. 1 is a flow chart of a preparation method of a multifunctional nanomaterial based on aggregation-induced emission and MXenes;
FIG. 2 is DSPE-PEG-NH 2 Schematic of wrapping UCNPs and AIE-PSs;
FIG. 3 is an absorption and emission spectrum of TBFT molecules;
FIG. 4 is a fluorescence spectrum of TBFT molecules in different ratios of THF/water;
FIG. 5 is the reactive oxygen species generating capacity of TUNPs and TUT NPs under white light/808 nm laser irradiation, respectively;
FIG. 6 is a graph of (A) TUT NPs at 808nm laser (1 w/cm 2 ) A temperature rise curve under irradiation; (B) Temperature rise curve of TUT NPs of 100 mug/mL under 808nm laser irradiation of different powers; (C) Using 808/nm laser (1 w/cm) 2 ) Repeatedly irradiating TUT NPs water solution to obtain a heating and cooling curve;
FIG. 7 shows TUT NPs at various concentrations in the dark, white or 808nm lasers (1 w/cm 2 ) Cytotoxicity generated under irradiation;
FIG. 8 is the effect of fluorescence imaging of mice at different time points;
FIG. 9 is (A) tumor inhibition rates for different treatment groups; (B) final treatment outcome for different treatment groups; (C) weight changes in mice in different treatment groups.
Detailed Description
The present invention will be described in further detail with reference to the following specific examples and drawings, but embodiments of the present invention are not limited thereto, and reference may be made to conventional techniques for process parameters that are not specifically noted.
Noun description
Photothermal therapy (PTT); photodynamic therapy (PDT); photoacoustic imaging (PAI); reactive Oxygen Species (ROS); photothermal Imaging Systems (PTIs); fluorescence imaging (FLI); photo-thermal conversion efficiency (PCE); photothermal therapeutic Photosensitizers (PSs); titanium carbide nanosheets (Ti) 3 C 2 NSs); near infrared one region (NIR-I750-1000 nm); tetrapropylammonium hydroxide (TPAOH)
TBFT 4,4' - ((5, 6-difluoroobenzo [ c ] [1,2,5] thiadiazole-4, 7-diyl) bis (thiophen-5, 2-diyl)) bis (N-phenyl-N- (4- (1, 2-triphenylvinyl) phenyl) aniline), under the name of tetraphenylethylene triphenylamine thiophene fluoro benzobisthiadiazole
As a result of the study by the inventors, the existing PSs with aggregation quenching (aggregation induced emission AIE) characteristics show prominence in fluorescence imaging navigation, which makes them Ti 3 C 2 However, most AIE-PSs are excited/emitted under ultraviolet/visible light (200-700 nm) with a shallow penetration depth, severely inhibiting their diagnostic effect, and with Ti 3 C 2 The NIR-I absorption ranges of the nanoplatelets are not matched. Since the development of AIE-PSs with a long wavelength optical window generally requires more steps of reaction and cumbersome purification process.
As shown in fig. 1, in order to solve the above technical problems, the present invention provides a method for preparing a multifunctional nanomaterial based on aggregation-induced emission and mxnes, wherein the method comprises the following steps:
s10, dispersing TBFT, rare earth up-conversion luminescent nano material and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-amino cross-linked substance in an organic solvent to obtain a mixed solution;
s20, adding the mixed solution into a phosphate buffer salt solution containing 1-ethyl 3- [ 3-dimethylaminopropyl ] carbodiimide hydrochloride and titanium carbide nano-sheets with carboxyl functions to react, so as to obtain the multifunctional nano-material based on aggregation-induced emission and MXnes.
In this embodiment, the DSPE-PEG-NH2 is an amphiphilic polymer, which self-assembles in water to form an inner hydrophobic and outer hydrophilic nanosphere, and UCNPS and AIE-PS are both hydrophobic materials and are encapsulated inside the nanosphere, as shown in fig. 2.
The rare earth up-conversion luminescent nanomaterial includes, but is not limited to: naYF 4 :Yb 3+ ，Er 3+ 、NaYF 4 :Yb 3+ ，Tm 3+ The method comprises the steps of carrying out a first treatment on the surface of the The organic solvents include, but are not limited to: tetrahydrofuran, acetonitrile and dimethyl sulfoxide.
In this example, nanoparticles (UCNPs) and AIE-PSs were up-converted by co-precipitation and encapsulated in amphiphilic polymer DSPE-PEG 2000 -NH 2 In nanospheres. As a typical MXees, ti 3 C 2 The surfaces of the nano-sheets are carboxylated in abundance by-NH 2 And amidation of-COOH, UCNPs, AIE-PSs and Ti 3 C 2 The nano-sheets are integrated into multiple piecesA functional nano platform. Covalent bonding of the polymer surface allows the nano-platform to have long-term stability and efficient tumor accumulation. The introduction of UCNPs enables NIR-I activation of AIE-PSs, significantly increasing the tissue penetration depth of phototherapy. AIE-PSs and Ti 3 C 2 The space isolation of the nanoplatelets is beneficial to inhibiting Ti 3 C 2 Quenching effect of nanoplatelets on photosensitizers. The method has excellent performance in FLI/PAI/PTI trimodal imaging guided PTT/PDT.
The multifunctional nanomaterial based on aggregation-induced emission and MXenes, and the preparation method and application thereof provided by the invention are further explained by specific examples.
Example 1
Preparation of titanium carbide nanosheets
Ti 3 AlC 2 Is obtained by mixing Ti, al and C powder in a molar ratio of 2:1:1 and ball milling for 10 h, and is pressed into round cakes under the pressure of 10 MPa. The discs were heated to 1500 ℃ for 2 hours in a tube furnace under flowing argon. The obtained Ti was then ground with a mortar and pestle 3 AlC 2 Crushing. About 10 of the g powder was then soaked in 80 ml of 40% HF aqueous solution at room temperature for 3 days. After centrifugation, the mixture was washed with deionized water and ethanol and stirred in 50 mL of TPAOH at room temperature for 3 d. Then Ti is added to 3 C 2 The nanosheet raw material was collected by centrifugation, washed 3 times with ethanol and water to remove residual TPAOH. Ultrasonic treatment for 30min to obtain small-sized Ti 3 C 2 A nano-sheet.
Example 2
Ti 3 C 2 Carboxyl function of nanoplatelets:
in diazonium salts of Ti 3 C 2 In a typical synthesis procedure with a reaction ratio of 5:1, 7 mmol of sodium hydroxide and 4-aminobenzoic acid were added to 80 mL of water to prepare a diazonium salt of a benzene carboxylic acid. Subsequently, the temperature was kept at 0-5℃and 7.6 mmol of sodium nitrite was slowly added. Finally, 6 mL of 20% HCl solution was added quickly and stirred for 45 minutes, the solution turned pale yellow in color. Then 25 mL diazonium salt solution was added to 1 mL Ti 3 C 2 Water-solubility of nanoplateletsThe solution (5 mg/mL) was stirred at 1000 rpm for 4 h. Ultrasonic treatment for 15 min, centrifuging at 9000 rpm for 30min, and washing with deionized water, acetone and ethanol sequentially.
Example 3
Preparation of TU NPs
1 mg TBFT, 6. Mu.L NaYF 4 :Yb 3+ 、Er 3+ UCNPs (5 mg/mL in THF) and 10 mg DSPE-PEG 2000 -NH 2 Added to 1 mL THF, left to stand for 30min, rapidly poured into 9 mL DI (deionized water) water, and then sonicated with a 45% output microprobe sonar for 2 minutes. The mixture was then transferred to a dialysis tube (MWCO 8000-14000 Da) and dialyzed in deionized water for 24 hours. To completely remove THF (tetrahydrofuran), fresh water was used instead of water every 4 hours. The TU NPs solution obtained finally was used after ultrafiltration concentration.
Example 4
Preparation of TUT NPs
TUT NPs are prepared from TU NPs and TCCH NSs by amide reaction. First, 10 mL PBS (phosphate buffered saline) containing EDC/NHS (1 mM/5 mM) and TCCH (10 g/mL) was stirred for 15 min, and then 1 mL TU NPs aqueous solution ([ TBFT ] = 0.5 mM) was added to the above solution. The solution was kept overnight in a shaker at 37 ℃. The heart was separated at 10000 rpm for 10 minutes and washed with DI water 3 times to obtain the product.
Application example 1
The compound TBFT prepared in example 1 was tested for its uv/vis absorption spectrum in THF and fluorescence spectrum in water, whose uv/vis absorption spectrum and fluorescence spectrum are shown in fig. 3; the aggregation-induced emission properties of TBFT (10. Mu.M) were evaluated in various ratios of THF/water mixtures and the AIE fluorescence spectra are shown in FIG. 4.
Application example 2: assessment of the Photoactive oxygen generating Capacity
The reactive oxygen species generating capacity of TBFT was evaluated using DCHF-DA as an indicator. 0.5 The mL of DCFH-DA (1X 10-3M) ethanol solution was added to 2 mL of NaOH (1X 10) -2 M) activating the solution to obtain DCFH, adding 10 mL PBS (pH 7.4) to adjust the pH value of the solution, and placing the solution in a dark place. DCFH (5. Mu.M) in PBS (pH 7.4) solution was usedThe TBFT NPs and TUT NPs (0.2 μm) were evaluated for their ability to generate active oxygen, irradiated with white light/808 nm laser light, and the fluorescence intensities at 525 nm at different time points under excitation of 488 nm were recorded using a fluorescence spectrophotometer, giving their fluorescence enhancement factors. As shown in FIG. 5, the irradiation of 808nm laser significantly enhanced the ROS production capability of TUT NPs.
Application example 3: photothermal capability assessment
TUT NPs and Ti at different concentrations 3 C 2 The aqueous nanoplatelet solution was continuously irradiated for 5 min under 808 and nm laser irradiation at different power densities, and the temperature was measured every 30 seconds until the temperature was nearly stable, as shown in fig. 6A and 6B. Using 808/nm laser (1 w/cm) 2 ) The TUT NPs aqueous solution was repeatedly irradiated for 50 minutes, real-time thermal imaging data was acquired every 30 seconds using FLIR E6 camera, and quantified by FLIR Examiner software, as shown in fig. 6C.
Application example 4: phototherapy antiproliferation experiment of diagnosis and treatment agent on breast cancer cell 4T1 cell
Test cells: breast cancer cells 4T1 cells; test drug: compound TBFT; light source: 808nm laser.
After digestion of cells in the logarithmic growth phase with pancreatin, the complete medium is resuspended into a cell suspension, which is then brought to 5X 10 3 Density of individual/wells was seeded in 96-well plates at 37 ℃,5% CO 2 Culturing in incubator, adding TUT NPs with different concentrations after 24, h to obtain final concentrations of 1,2,5, 10, 15, 20, 50 μg/mL, culturing 4 h, and illuminating (power 1W/cm) 2 808nm laser for 10 min) under the same experimental conditions, the study of dark toxicity was also performed in the experimental group without illumination. After incubation 4 h, wash 3 times with PBS solution, followed by incubation 2 h with fresh 10% CCK-8 FBS free medium in dark conditions, and then measurement of absorbance (OD) at 450 nm with a microplate reader, the corresponding cell viability was calculated by the following formula: cell viability (%) = (OD sample-OD background)/(OD control-OD background) ×100%. The experimental results are shown in FIG. 7, and the cell viability of the control group which is not irradiated by laser is more than 95% when the drug concentration is 50 mu M, which proves that the compoundThe dark toxicity of the product is smaller, and the biocompatibility is better. The cell survival rate of TUT NPs of the experimental group is only 7% under the irradiation of laser, which proves that the TUT NPs have obvious phototherapy antiproliferation effect on breast cancer cells 4T1 cells
Application example 5: fluorescence imaging experiment of diagnosis and treatment agent in 4T1 tumor mice
The transplanted tumor 4T1 mice were anesthetized with 2% isoflurane 2L/min oxygen flow, and TUT NPs (200 μL, 1 mM) were then injected intratumorally. In vivo near infrared one-region fluorescence imaging was obtained at predetermined time intervals (3, 6, 12, 24, 36, 48, 72, and 96 hours) after injection using an IVIS spectroscopic imaging system (PerkinElmer) and a commercial series II 900/1700 imaging system, as shown in fig. 8.
Application example 6: therapeutic agent for in vivo phototherapy antiproliferation experiments of 4T1 tumor mice
The transplanted tumor 4T1 tumor mice were randomly divided into 6 groups (5 mice per group, including saline group, TUT NPs group, TU NPs group, TUT nps+ laser group, and TUT nps+ laser group) when tumor volume reached 100 mm 3 After that, 200. Mu.L of the corresponding drug was injected by tail vein injection. 36 Following h intratumoral injection, each group of mice was continuously tumor-injected with 808nm laser (1W/cm 2 ) The treatment is carried out by irradiation for 10 min. Tumor size and body weight were recorded for each mouse every three days after various treatments. Tumor volume was measured with vernier calipers as per the general formula v= (tumor length x tumor width) 2 ) And (2) calculating. Relative volume V/V 0 (V 0 Initial volume of tumor prior to treatment) reflects the relative tumor growth ratio. The experimental results are shown in fig. 9, and the tumor growth inhibition rate of the TUT nps+ laser group is maximum and reaches more than 90%.
The foregoing has described the basic principles, main features and performance advantages of the present invention. It is to be understood that the nature and application of the present invention is not limited to the examples described above, and that modifications and variations thereof will be apparent to those skilled in the art in light of the foregoing disclosure and it is intended to cover all such modifications and variations as fall within the purview of the appended claims.
Claims (8)
1. The preparation method of the multifunctional nanomaterial based on aggregation-induced emission and MXenes is characterized by comprising the following steps of:
dispersing TBFT, rare earth up-conversion luminescent nano material and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-amino cross-linked substance in an organic solvent, standing, injecting into deionized water after standing, and sequentially carrying out ultrasonic and dialysis treatment to obtain nanospheres with amino groups on the surfaces;
adding the nanospheres with the amino groups on the surfaces into phosphate buffer salt solution of titanium carbide nano sheets with carboxyl functions, and obtaining the multifunctional nano material based on aggregation-induced emission and MXenes through amidation reaction of the amino groups and the carboxyl groups;
the organic solvent is selected from one of tetrahydrofuran, acetonitrile and dimethyl sulfoxide;
the TBFT is 4,4' - ((5, 6-difluorobenzo [ c ] [1,2,5] thiadiazole-4, 7-diyl) bis (thiophene-5, 2-diyl) bis (N-phenyl-N- (4- (1, 2-triphenylvinyl) phenyl) aniline).
2. The method according to claim 1, wherein the rare earth up-conversion luminescent nanomaterial is NaYF 4 :Yb 3+ ，Er 3+ Or NaYF 4 :Yb 3+ ，Tm 3+ 。
3. The preparation method of claim 1, wherein the steps of dispersing the TBFT, the rare earth up-conversion luminescent nanomaterial and the distearoyl phosphatidylethanolamine-polyethylene glycol 2000-amino cross-linked substance in an organic solvent, and sequentially performing ultrasonic and dialysis treatment to obtain the nanospheres with the amino groups on the surfaces, specifically comprising:
dispersing TBFT, rare earth up-conversion luminescent nano material and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-amino cross-linked substance in an organic solvent, standing, and injecting into deionized water after standing;
carrying out ultrasonic treatment by adopting a microprobe sonar device to obtain a mixture;
transferring the mixture into a dialysis tube, and dialyzing in deionized water to remove the organic solvent, thereby obtaining the nanospheres with amino groups on the surfaces.
4. The preparation method according to claim 1, wherein the step of adding the nanospheres with amino groups on the surface into a phosphate buffer salt solution containing titanium carbide nanoplatelets with carboxyl functions for reaction to obtain the multifunctional nanomaterial based on aggregation-induced emission and mxnes specifically comprises the following steps:
stirring a phosphate buffer solution containing 1-ethyl 3- [ 3-dimethylaminopropyl ] carbodiimide hydrochloride, N-hydroxysuccinimide and titanium carbide nano-sheets with carboxyl functions for 10-30min;
adding nanospheres with amino groups on the surfaces into the phosphate buffer solution, preserving overnight in a shaking table at 37 ℃, and sequentially carrying out centrifugal separation and deionized water washing to obtain the multifunctional nanomaterial based on aggregation-induced emission and MXenes.
5. The method according to claim 1, wherein the method for producing titanium carbide nanoplatelets having a carboxyl function comprises:
dispersing sodium hydroxide and 4-aminobenzoic acid in water to obtain a benzene carboxylic acid diazonium salt water solution;
maintaining the temperature of the benzene carboxylic acid diazonium salt water solution at 0-5 ℃, and sequentially adding sodium nitrite and hydrochloric acid to obtain a pale yellow solution;
and adding the pale yellow solution into the aqueous solution of the titanium carbide nanosheets, and sequentially stirring, carrying out ultrasonic treatment and centrifugal treatment to obtain the titanium carbide nanosheets with the carboxyl function.
6. A multifunctional nanomaterial based on aggregation-induced emission and mxnes, characterized in that it is prepared by the preparation method according to any one of claims 1 to 5.
7. The use of the aggregation-induced emission and mxnes-based multifunctional nanomaterial according to claim 6, for the preparation of a tumor imaging agent as a nanosensing agent.
8. The use of the aggregation-induced emission and mxnes-based multifunctional nanomaterial according to claim 6, as an antitumor drug.
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