CN114288357B - Dendrobium officinale product and preparation method thereof - Google Patents
Dendrobium officinale product and preparation method thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention relates to the technical field of natural resource development and utilization, in particular to a dendrobium candidum product and a preparation method thereof, comprising the following preparation steps of dendrobium candidum extract: cutting fresh dendrobium candidum leaves into small pieces to obtain dendrobium candidum small pieces; performing microwave treatment on the dendrobium nobile to obtain a dendrobium nobile after microwaves; adding the dendrobium nobile slices subjected to microwave treatment and cellulase into water to obtain an enzymolysis system; after enzymolysis treatment, adding water into an enzymolysis system, and then performing hot-dip extraction to obtain dendrobium extract; concentrating the dendrobe extract to obtain dendrobe extract. The technical problem that dendrobe leaves among the prior art are difficult to by make full use of has been solved to this scheme. Can realize the full utilization of the dendrobium leaves, form new products related to the dendrobium leaves, improve the comprehensive utilization rate of dendrobium resources and have wide application prospect.
Description
Technical Field
The invention relates to the technical field of natural resource development and utilization, in particular to a dendrobium candidum product and a preparation method thereof.
Background
Dendrobe is one of the largest genus of orchid, and is widely distributed in tropical and subtropical areas such as asia, europe and oceangoin, and has a total of one thousand to five hundred worldwide. About 76 kinds of Chinese medicines are distributed in southwest, eastern China and south China. Herba Dendrobii has effects of nourishing yin, clearing heat, benefiting stomach, promoting fluid production, moistening lung, relieving cough, etc., and is commonly used for treating various diseases such as heat disease, body fluid injury, dry mouth, polydipsia, deficiency heat after illness, etc. Modern pharmacological researches show that the main functional component of dendrobium is water-soluble polysaccharide which can obviously enhance immune response, has the effects of preventing and resisting cancer, resisting aging, resisting oxidation and resisting radiation, and can be widely applied to medicines, functional foods and cosmetics.
The development and utilization of dendrobe become hot spots for research and application development in the disciplinary field, novel pharmaceutical preparations with different functions and functional foods are commercialized. The dendrobium has various varieties, the dendrobium polysaccharide structure research of different varieties, the dendrobium polysaccharide chemical synthesis research, the dendrobium polysaccharide pharmacological, physiological function and mechanism research, the dendrobium germplasm resource screening and the like are trends of the dendrobium for research, development and utilization in future. The dendrobium leaves have lower content of functional polysaccharide and higher development and utilization cost, so that a large amount of dendrobium leaves are abandoned, and the basic and application researches of the dendrobium leaves are far behind the researches of dendrobium stems. How to fully utilize dendrobium leaves, research the active ingredients of the dendrobium leaves, develop related new products and improve the comprehensive utilization rate of dendrobium resources is a problem to be solved urgently in the field.
Disclosure of Invention
The invention aims to provide a dendrobium candidum product to solve the technical problem that dendrobium candidum leaves in the prior art are difficult to fully utilize.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a preparation method of dendrobium candidum products comprises the following preparation steps of dendrobium candidum extract: cutting fresh dendrobium candidum leaves into small pieces to obtain dendrobium candidum small pieces; performing microwave treatment on the dendrobium nobile to obtain a dendrobium nobile after microwaves; adding the dendrobium nobile slices subjected to microwave treatment and cellulase into water to obtain an enzymolysis system; after enzymolysis treatment, adding water into an enzymolysis system, and then performing hot-dip extraction to obtain dendrobium extract; concentrating the dendrobe extract to obtain dendrobe extract.
The principle and the advantages of the scheme are as follows: according to the technical scheme, the dendrobium leaves are used as raw materials for extracting polysaccharide, the dendrobium leaves which are difficult to fully utilize are prepared into usable products, and the problem that the comprehensive utilization rate of dendrobium resources is not ideal is solved. In the technical scheme, the dendrobium leaves are treated by microwaves, cellulose substances of the dendrobium leaves are degraded by an enzymolysis method, and the dendrobium polysaccharide extraction rate is ideal through hot dipping extraction. The treatment method combining the microwave treatment and the enzymolysis treatment plays a very important role in improving the extraction rate of dendrobium candidum polysaccharide and improving the process stability. The use of microwave energy to increase extraction is a state of the art. In the microwave field, the difference in the ability to absorb microwaves causes certain areas of the matrix material to be selectively heated, thereby causing the extracted material to separate from the matrix. The microwave extraction has the characteristics of simple equipment, wide application range, high extraction efficiency, good repeatability, time saving, reagent saving, small pollution and the like, and is very suitable for industrial production. In the technical scheme, the blade before enzymolysis treatment is firstly treated for a short time by microwaves, so that the target components in the blade are easy to be extracted by subsequent hot dipping. In addition, the enzymolysis treatment mode can fully destroy the cell walls of the leaves, so that the target components are easier to leach out in hot-dip extraction. However, the inventors found that the stability of the process using only the enzymolysis treatment is not ideal, and the effect of the enzymolysis treatment is affected by various factors such as the contact condition of the enzyme with the material, the enzyme activity condition, and the like. The inventors analyzed that the easier contact between enzyme and substrate after microwaving the leaves, each enzymatic treatment could assist the performance of the hot dip extraction more stably and efficiently.
In the technical scheme, the dendrobium leaves are taken as raw materials of the product, the dendrobium leaves soluble polysaccharide is extracted through combination of various auxiliary means, the obtained dendrobium extract can be used for developing foods, medicines or cosmetics, and theoretical basis and application support are provided for development of related products. Through the technical scheme, waste resources can be fully utilized, the added value of the dendrobium is improved, and meanwhile, the income of dendrobium planting farmers can be increased.
Further, the parameters of the microwave treatment are as follows: the power is 800-1000W and the time is 50-60s.
By adopting the technical scheme, under the microwave condition, the extraction rate of the dendrobium polysaccharide can be improved, and the ideal effect of improving the process stability can be obtained. Wherein 800-1000W is the power commonly used for microwave treatment, and the ideal effect can be obtained by using common equipment (such as a common microwave oven). Through tests, if the treatment time is too long, the extraction rate of dendrobium polysaccharide is not greatly improved, and the process stability is reduced.
Further, in the enzymolysis system, the mass fraction of the cellulase is 2-3%, and the water is used in the following amount: 20-50ml of water is correspondingly used for each g of dendrobium nobile tablets.
By adopting the technical scheme, the cellulose components in the leaves can be fully decomposed by adopting the dosage of the cellulase, and the subsequent hot-dip extraction process is assisted.
Further, parameters of enzymolysis treatment are as follows: the temperature is 30-35 ℃, the time is 2-3h, and the pH value is 6-7.
By adopting the technical scheme, under the conditions of the temperature, the time and the pH value, the enzymolysis enzyme activity is higher, and the effect is more ideal.
Further, the water is added into the enzymolysis system in an amount of 20-50ml of water for each g of dendrobium nobile; the parameters of the hot dip extraction are: the temperature is 80-90 ℃ and the time is 2-3h.
By adopting the technical scheme, the soluble polysaccharide in the dendrobium leaves can be fully dissolved out by adopting the parameter conditions of hot-dip extraction.
Further, the preparation method also comprises the following steps of: cutting fresh fructus Lycii, hot-soaking with water as solvent to obtain fructus Lycii extractive solution, and concentrating the fructus Lycii extractive solution to obtain fructus Lycii extract; the dosage ratio of fresh medlar to water is 1g:50-60ml; the parameters of the hot dip extraction are: the temperature is 80-90 ℃ and the time is 2-3h.
By adopting the technical scheme, the medlar soluble polysaccharide can be extracted by adopting the method, and the polysaccharide has the effects of regulating immunity, resisting aging and resisting cancer.
Further, the preparation method also comprises the steps of: pulverizing rhizoma Dioscoreae decoction pieces, adding water, boiling for 15-30min, filtering to obtain rhizoma Dioscoreae slurry, wherein the dosage ratio of rhizoma Dioscoreae decoction pieces to water is 1g:50-60ml; adding water-soluble starch, gelatin and arabinose into the yam pulp to obtain mixed pulp; in the mixed slurry, the mass percentages of the water-soluble starch, the gelatin and the arabinose are respectively 2-3%, 0.5-0.8% and 0.1-0.2%.
By adopting the technical scheme, the mixed slurry is used as an auxiliary material for preparing the dendrobium candidum product, the granulation process of the dendrobium candidum extract can be realized under the action of the yam extract, the water-soluble starch, the gelatin and the arabinose, dry particles are formed, and a user can dissolve and drink the dendrobium candidum extract by himself when drinking is needed.
Further, the method also comprises a granulating step: adding the mixed slurry into the mixed extract formed by the dendrobium extract and the medlar extract, and homogenizing to form a soft material; the mass ratio of the dendrobium extract to the medlar extract to the mixed slurry is 8-10:1-2:3-4; the soft material is granulated, dried and granulated to obtain the dendrobium candidum product.
By adopting the technical scheme, the dendrobium extract (the main component is the dendrobium soluble polysaccharide) and the medlar extract (the main component is the medlar soluble polysaccharide) are combined, so that the advantages of the dendrobium polysaccharide and the medlar polysaccharide can be fully exerted, and the nutritional components of the product are more abundant. In addition, the mixed slurry ensures that the dendrobium extract and the medlar extract can be processed into granules, and is convenient for packaging, transportation and carrying.
The scheme also provides the dendrobium candidum product obtained by the preparation method of the dendrobium candidum product.
By adopting the technical scheme, the dendrobium candidum product of the scheme is rich in nutrition and fully utilizes the dendrobium candidum leaf resources.
Further, under the condition of 50 ℃, the dissolution time of 10g of dendrobium candidum products in 200ml of water is 88-101s.
By adopting the technical scheme, the dendrobium candidum product has good solubility, can realize quick dissolution and is convenient to take.
Detailed Description
The following is a further detailed description of the embodiments:
example 1
S1, preparing dendrobe extract:
fresh dendrobium candidum leaves are taken, cut into small pieces with the thickness of 1cm multiplied by 1cm, and the small pieces are spread in a microwave oven, and the thickness of the formed small piece layers is 0.5cm (the optional range is 0.5cm to 1 cm). And (3) carrying out microwave treatment on the small pieces for 50s (optional range 50-60 s) under the condition of microwave power 1000W (optional range 800-1000W) to obtain the dendrobium nobile small pieces after microwave treatment. Placing the dendrobium nobile subjected to microwave treatment in pure water, and adding cellulase (CAS: 9012-54-8) to obtain an enzymolysis system. The ratio of the dendrobium nobile (calculated by the mass of the original dendrobium nobile) to the pure water is 1g:30ml (optional range 1g:20-30 ml), the mass fraction of cellulase in the enzymolysis system is 2% (optional range 2-3%), and the enzymolysis is carried out for 2h (optional range 2-3 h) under the conditions of 30 ℃ (optional range 30-35 ℃) and pH of 6 (optional range 6-7). Adding pure water into the enzymolysis system, wherein the ratio of the dendrobium nobile (calculated by the mass of the initial dendrobium nobile) to the supplementary adding amount of the pure water is 1g:50ml (optional range 1g:20-50 ml), hot dip extracting at 80deg.C (optional range 80-90deg.C) for 3 hr (optional range 2-3 hr), and filtering to obtain filtrate. And performing hot dip extraction on the filter residues for one time, wherein each g of dendrobium nobile corresponds to 80ml (the optional range is 40-80 ml) of pure water according to the mass of the dendrobium nobile, filtering to obtain filtrate, and combining the filtrate to obtain dendrobium nobile extract. And (3) carrying out reduced pressure distillation on the dendrobium extract to obtain dendrobium extract, wherein the relative density of the dendrobium extract is 1.08.
S2, preparing wolfberry extract:
taking fresh wolfberry fruits, cutting the fresh wolfberry fruits, and extracting the fresh wolfberry fruits by using pure water under the following parameter conditions: feed liquid ratio 1g:50ml (optional range 1g:50-60 ml), 80 ℃ (optional range 80-90 ℃), 3h (optional range 2-3 h). The hot dip extraction is performed twice and the filtrates are combined (called wolfberry extract). Distilling fructus Lycii extractive solution under reduced pressure to obtain fructus Lycii extract with relative density of 1.10.
S3, preparing mixed slurry:
taking yam decoction pieces, crushing the yam decoction pieces, passing through 80 meshes to obtain yam powder, adding pure water into the yam powder for extraction, wherein the ratio of the material to the liquid is 1g:60ml (optional range 1g:50-60 ml), boiling for 30min (optional range 15-30 min), and filtering to obtain rhizoma Dioscoreae slurry. Adding water-soluble starch, gelatin and arabinose into rhizoma Dioscoreae slurry, and homogenizing to obtain mixed slurry. The mass fraction of the water-soluble starch, gelatin and arabinose in the mixed slurry is 3% (optional range 2-3%), 0.8% (optional range 0.5-0.8%) and 0.2% (optional range 0.1-0.2%).
S4 granulating
Mixing herba Dendrobii extract and fructus Lycii extract at a certain proportion, adding the mixed slurry into the above extract, stirring while adding, and mixing to obtain soft material. Wherein, the mass ratio of the dendrobium extract, the medlar extract and the mixed slurry is 8:2:3 (the optional range is 8-10:1-2:3-4). Granulating the soft material by using a swing type granulator with a 40-mesh screen, drying, and finishing to obtain the dendrobium candidum product.
Example 2
S1, preparing dendrobe extract:
fresh dendrobium candidum leaves are taken, cut into small pieces with the thickness of 1cm multiplied by 1cm, and the small pieces are spread in a microwave oven, so that the thickness of the formed small piece layers is 1cm. And carrying out microwave treatment on the small pieces for 60s under the condition of microwave power of 800W to obtain the dendrobium nobile small pieces after microwave treatment. Placing the dendrobium nobile subjected to microwave treatment in pure water, and adding cellulase (CAS: 9012-54-8) to obtain an enzymolysis system. The dosage ratio of the dendrobium nobile tablets to the pure water is 1g:20ml, wherein the mass fraction of the cellulase in the enzymolysis system is 3%, and the enzymolysis is carried out for 1h at 35 ℃ and pH of 7. Adding pure water into the enzymolysis system, wherein the proportion of the supplementary addition amount of the dendrobium nobile tablets and the pure water is 1g:20ml, hot-dip extracting at 90deg.C for 2 hr, and filtering to obtain filtrate. And performing hot dip extraction on the filter residues for one time, wherein the mass of each gram of dendrobium nobile corresponds to 80ml of pure water, filtering to obtain filtrate, and combining the filtrate to obtain dendrobium nobile extract. And (3) carrying out reduced pressure distillation on the dendrobium extract to obtain dendrobium extract, wherein the relative density of the dendrobium extract is 1.10.
S2, preparing wolfberry extract:
taking fresh wolfberry fruits, cutting the fresh wolfberry fruits, and extracting the fresh wolfberry fruits by using pure water under the following parameter conditions: feed liquid ratio 1g:60ml,90℃for 2h. The hot dip extraction is performed twice and the filtrates are combined (called wolfberry extract). Distilling fructus Lycii extractive solution under reduced pressure to obtain fructus Lycii extract with relative density of 1.08.
S3, preparing mixed slurry:
taking yam decoction pieces, crushing the yam decoction pieces, passing through 120 meshes to obtain yam powder, adding pure water into the yam powder for extraction, wherein the ratio of the material to the liquid is 1g:50ml, boiling for 15min, filtering to obtain liquid phase, and obtaining rhizoma Dioscoreae slurry. Adding water-soluble starch, gelatin and arabinose into rhizoma Dioscoreae slurry, and homogenizing to obtain mixed slurry. The mass fraction of the water-soluble starch, gelatin and arabinose in the mixed slurry was 2%, 0.5% and 0.1%.
S4 granulating
Mixing herba Dendrobii extract and fructus Lycii extract at a certain proportion, adding the mixed slurry into the above extract, stirring while adding, and mixing to obtain soft material. Wherein the mass ratio of the dendrobium extract to the medlar extract to the mixed slurry is 10:1:4. Granulating the soft material by using a swing type granulator with a 40-mesh screen, drying, and finishing to obtain the dendrobium candidum product.
Comparative example 1
This comparative example is basically the same as example 1, except that S1 is adjusted, specifically:
s1, preparing dendrobe extract:
fresh dendrobium leaves are taken, cut into 1cm multiplied by 1cm pieces, the dendrobium pieces are placed in pure water, and cellulase (CAS: 9012-54-8) is added to obtain an enzymolysis system. The dosage ratio of the dendrobium nobile tablets to the pure water is 1g:30ml, wherein the mass fraction of the cellulase in the enzymolysis system is 2%, and the enzymolysis is carried out for 2 hours at 30 ℃ and pH of 6. Adding pure water into the enzymolysis system, wherein the proportion of the supplementary addition amount of the dendrobium nobile tablets and the pure water is 1g:50ml, hot-dip extracting at 80deg.C for 3 hr, and filtering to obtain filtrate. And performing hot dip extraction on the filter residues for one time, wherein the mass of the dendrobium nobile is 80ml of pure water for each g of dendrobium nobile, filtering to obtain filtrate, and merging the filtrates. And (3) carrying out reduced pressure distillation on the combined filtrate to obtain dendrobium extract, wherein the relative density of the dendrobium extract is 1.10.
Comparative example 2
This comparative example is basically the same as example 1, except that S1 is adjusted, specifically:
s1, preparing dendrobe extract:
fresh dendrobium leaves are taken, cut into small pieces with the thickness of 1cm multiplied by 1cm, and the small pieces are spread in a microwave oven, so that the thickness of the formed small piece layers is 0.5cm. And carrying out microwave treatment on the small pieces for 50s under the condition of microwave power of 1000W to obtain the dendrobium nobile small pieces after microwave treatment. Placing the dendrobium nobile subjected to microwave treatment in pure water, wherein the dosage ratio of the dendrobium nobile (calculated by the mass of the initial dendrobium nobile) to the pure water is 1g:80ml, hot-dip extracting at 80deg.C for 3 hr, and filtering to obtain filtrate. And performing hot dip extraction on the filter residues for one time, wherein the mass of the dendrobium nobile is 80ml of pure water for each g of dendrobium nobile, filtering to obtain filtrate, and merging the filtrates. And carrying out reduced pressure distillation on the combined filtrate to obtain dendrobium extract, wherein the relative density of the dendrobium extract is 1.09.
Comparative example 3
This comparative example is basically the same as example 1, except that S2 and S4 are adjusted, specifically:
s2, preparing wolfberry extract; s4, when the soft material is prepared, the medlar extract is not added, and the mass ratio of the dendrobium extract to the mixed slurry is 8:3.
Comparative example 4
The comparative example is basically the same as example 1 except that in S1, the microwave treatment time is selected to be 80S.
Experimental example 1: extraction efficiency research of dendrobe polysaccharide
The experimental example adopts a phenol sulfuric acid method to measure the polysaccharide content in dendrobium leaves and dendrobium extractum. The specific test steps are as follows: first, a standard curve is drawn using a glucose standard. Accurately weighing 150mg of dry constant weight glucose, placing into a 500mL volumetric flask, adding distilled water for dissolution, and fixing the volume; accurately sucking 0.00, 0.20, 0.30, 0.40, 0.50, 0.60 and 0.70mL of the prepared glucose solution, and placing the glucose solution in a test tube with a plug; adding distilled water to make the volume 2mL, adding 1mL of 5% phenol solution, shaking uniformly, rapidly adding 5mL of concentrated sulfuric acid, shaking uniformly, and standing for 5min; the mixture was placed in a water bath at 90℃for 15min, cooled at room temperature, absorbance was measured at 490nm wavelength, and a standard curve was drawn. Drying fresh dendrobium candidum leaves in an oven at 80 ℃ for one day, crushing the dried leaves, and sieving the crushed leaves with a 80-mesh sieve. Accurately weighing 0.2g of leaf powder, reflux-extracting with 30mL of petroleum ether for 20min, and volatilizing petroleum ether. Then, the mixture was extracted with 100mL of ethanol having a volume fraction of 85% under reflux for 1 hour. The residue was then extracted with 100ml of water under reflux for 1h, and the filtrate (fresh leaf sample to be tested) was collected. And then, carrying out phenol sulfuric acid method measurement on a sample to be measured of the fresh blade, measuring absorbance at a wavelength of 490nm, calculating by a standard curve, and converting the absorbance into polysaccharide content in the blade. The fresh dendrobium leaves used in the embodiment, the comparative example and the experimental example are the same batch, and the polysaccharide content (average value is obtained by repeating three times in mass percent) in the fresh dendrobium leaves of the batch is tested. The method comprises the steps of detecting polysaccharide content of extract, namely only dissolving dendrobe extract in pure water, dispersing and dissolving 1g of extract by using 10ml of pure water, then measuring absorbance at 490nm wavelength by using a phenol sulfuric acid method, calculating and converting the absorbance into polysaccharide content in dendrobe extract by using a standard curve, and calculating the extraction rate of polysaccharide substances, wherein the calculation formula is as follows: (content of polysaccharide in dendrobe extract x mass of dendrobe extract obtained from fresh leaves)/(mass of fresh leaves x average value of polysaccharide content in fresh dendrobe leaves) ×100%.
Experimental results referring to table 1, the dendrobium extracts prepared in example 1, example 2, comparative example 1, comparative example 2 and comparative example 4 were selected for detection in this experimental example. Referring to the preparation method of the dendrobium extract of the embodiment or the comparative example, six preparation steps (parallel experiments 1-3) are respectively repeated, six dendrobium extract samples are obtained in each corresponding embodiment or comparative example, the content of polysaccharide in the dendrobium extract is measured in each dendrobium extract sample, and the extraction rate of polysaccharide substances is obtained through conversion.
Table 1: extraction yield study of different extraction schemes (cv=sd/mean×100%)
As can be seen from the data in table 1, in examples 1 and 2, the polysaccharide substances in the dendrobe leaves can be extracted more fully by performing the microwave treatment and then the enzymatic extraction treatment on the dendrobe leaves. The extraction rate of the polysaccharide of the dendrobium extract obtained by repeating the extraction process for six times is stable in value and small in deviation, which shows that the process stability of the embodiment 1 and the embodiment 2 is good, the method is suitable for process expansion, and the method is expected to be applied to industrial mass production. Comparative example 1 was not subjected to microwave treatment, and was subjected to enzymolysis and extraction directly, the extraction rate was lowered to some extent, and the deviation value of six parallel tests was very large, which means that the step of microwave treatment was not adopted, and the process stability was negatively affected. Comparative example 2 does not use an enzymatic hydrolysis means, resulting in a very significant decrease in extraction yield. Comparative example 4 increased the microwave treatment time without much difference in extraction yield from example 1 and example 2, but had poor process stability, and was not suitable for practical production applications.
Experimental example 2: dissolution property research of dendrobium candidum products
The experimental example is carried out by referring to the solubility detection method of the granule of the rule 0104 in the Chinese pharmacopoeia 2015 (four parts). Taking 10g of dendrobium candidum product, then putting into 200ml of water at 50 ℃, stirring and recording the time for completely dissolving particles (dendrobium candidum product). The results of the experiments are shown in Table 2, and each sample experiment was repeated 3 times.
Table 2: dissolution time measurement results
The "×" in table 2 shows that the t-test was performed separately from example 1 and example 2, with p less than 0.01, and significant. Experimental results show that the dendrobium candidum product prepared by the scheme has good solubility, and when the dendrobium candidum product prepared solution is drunk, solid particles can be dissolved in water rapidly, so that the dendrobium candidum product is convenient to prepare. Comparative example 1 did not subject dendrobium candidum to microwave treatment, resulting in slightly deteriorated solubility of the obtained product. In addition to the extraction method, the addition of the wolfberry extract also has a certain positive effect on the solubility of the dendrobium candidum product, and comparative example 3 does not add the wolfberry extract into the dendrobium candidum product, so that the solubility of the finally obtained dendrobium candidum product is poor. The inventor adds a small amount of wolfberry extract in consideration of enriching nutrient components when developing the product, and surprisingly discovers that the wolfberry extract has the effect of improving the dissolution performance of the whole dendrobium candidum product, namely the wolfberry extract of the scheme has certain dissolution assisting performance.
The foregoing is merely exemplary of the present invention, and specific technical solutions and/or features that are well known in the art have not been described in detail herein. It should be noted that, for those skilled in the art, several variations and modifications can be made without departing from the technical solution of the present invention, and these should also be regarded as the protection scope of the present invention, which does not affect the effect of the implementation of the present invention and the practical applicability of the patent. The protection scope of the present application shall be subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (3)
1. The preparation method of the dendrobium candidum product is characterized by comprising the following preparation steps of dendrobium candidum extract: cutting fresh dendrobium candidum leaves into small pieces to obtain dendrobium candidum small pieces; performing microwave treatment on the dendrobium nobile to obtain a dendrobium nobile after microwaves; adding the dendrobium nobile slices subjected to microwave treatment and cellulase into water to obtain an enzymolysis system; after enzymolysis treatment, adding water into an enzymolysis system, and then performing hot-dip extraction to obtain dendrobium extract; concentrating the dendrobium extract to obtain dendrobium extract;
the parameters of the microwave treatment are as follows: the power is 800-1000W, and the time is 50-60s;
in the enzymolysis system, the mass fraction of the cellulase is 2-3%, and the water consumption is as follows: 20-50ml of water is correspondingly used for each g of dendrobium nobile tablets;
the parameters of enzymolysis treatment are as follows: the temperature is 30-35 ℃, the time is 2-3h, and the pH value is 6-7;
the water is added into the enzymolysis system in an amount of 20-50ml of water for each g of dendrobium nobile; the parameters of the hot dip extraction are: the temperature is 80-90 ℃ and the time is 2-3 hours;
the preparation method also comprises the preparation steps of the wolfberry extract: cutting fresh fructus Lycii, hot-soaking with water as solvent to obtain fructus Lycii extractive solution, and concentrating the fructus Lycii extractive solution to obtain fructus Lycii extract; the dosage ratio of fresh medlar to water is 1g:50-60ml; the parameters of the hot dip extraction are: the temperature is 80-90 ℃ and the time is 2-3 hours;
the preparation method also comprises the preparation steps of the mixed slurry: pulverizing rhizoma Dioscoreae decoction pieces, adding water, boiling for 15-30min, filtering to obtain rhizoma Dioscoreae slurry, wherein the dosage ratio of rhizoma Dioscoreae decoction pieces to water is 1g:50-60ml; adding water-soluble starch, gelatin and arabinose into the yam pulp to obtain mixed pulp; in the mixed slurry, the mass percentages of the water-soluble starch, the gelatin and the arabinose are respectively 2-3%, 0.5-0.8% and 0.1-0.2%;
the method also comprises the step of granulating: adding the mixed slurry into the mixed extract formed by the dendrobium extract and the medlar extract, and homogenizing to form a soft material; the mass ratio of the dendrobium extract to the medlar extract to the mixed slurry is 8-10:1-2:3-4; the soft material is granulated, dried and granulated to obtain the dendrobium candidum product.
2. The dendrobium candidum product obtained by the preparation method of the dendrobium candidum product according to claim 1.
3. The dendrobium candidum product obtained by the preparation method of the dendrobium candidum product according to claim 2, wherein the dissolution time of 10g of the dendrobium candidum product in 200ml of water is 88-101s at 50 ℃.
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