CN114264590A - Detachable portable sensor for cell detection, assembling method, measuring method and designing method - Google Patents
Detachable portable sensor for cell detection, assembling method, measuring method and designing method Download PDFInfo
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- CN114264590A CN114264590A CN202111524332.6A CN202111524332A CN114264590A CN 114264590 A CN114264590 A CN 114264590A CN 202111524332 A CN202111524332 A CN 202111524332A CN 114264590 A CN114264590 A CN 114264590A
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 34
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 22
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- 229910052737 gold Inorganic materials 0.000 claims abstract description 22
- 238000007654 immersion Methods 0.000 claims abstract description 20
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- 238000004891 communication Methods 0.000 claims description 3
- 238000002847 impedance measurement Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 238000003556 assay Methods 0.000 claims 1
- 238000013461 design Methods 0.000 abstract description 15
- XOMKZKJEJBZBJJ-UHFFFAOYSA-N 1,2-dichloro-3-phenylbenzene Chemical compound ClC1=CC=CC(C=2C=CC=CC=2)=C1Cl XOMKZKJEJBZBJJ-UHFFFAOYSA-N 0.000 description 10
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- 230000005684 electric field Effects 0.000 description 4
- 238000000691 measurement method Methods 0.000 description 3
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- 238000007689 inspection Methods 0.000 description 2
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Abstract
The application discloses a detachable portable sensor for cell detection, an assembling method, a measuring method and a designing method. The design key points are as follows: the SMA connector electrode comprises a main body structure and an SMA connector electrode; the main body structure comprises: an upper structure, a lower structure; the upper structure comprises an upper PCB and an upper cover which are stacked from top to bottom; the lower structure includes: a sealing gasket, a lower PCB and a base; a circumferential electrode is arranged in the accommodating cavity; the bottom of the accommodating cavity is provided with an opening; x holes are formed in the sealing gasket, X gold immersion electrodes are also formed on the lower PCB, and X represents any natural number; SMA joint electrodes are arranged in each group of SMA joint holes of the upper PCB and 1 group of SMA joint holes on the lower PCB. By adopting the detachable portable sensor for cell detection, the assembling method, the measuring method and the design method, the detection precision can be effectively improved.
Description
Technical Field
The application relates to the technical field of sensor electronic components, in particular to a detachable portable sensor for cell detection, an assembly method, a measurement method and a design method.
Background
The portable sensor is a device for detecting the electrical impedance of cells and is applied to the medical field.
The following documents describe the prior art of cell detection impedance sensors in detail.
Prior art document 1: xuying, yangyong, wangping, development of cellular electrical impedance sensors based on the BIOMEMS technique [ J ] international journal of biomedical engineering, 2008,31(003): 145-: the cell electrical impedance sensor has the advantages of novel design, long-time nondestructive testing and the like, and can be applied to the morphological and functional research of microorganisms such as cells and the like.
Prior document 2: zhang Xiao Cheng et al, progress of the inter-digital microelectrode impedance sensor research for cell detection [ J ] analytical laboratories, 2018,04(v.37): 109-.
Prior document 3: heing, trekking, gomperoth, etc. the research progress of micro-interdigital electrode impedance biosensors for cell detection [ J ] the academic press for food safety quality detection, 2018, can be known from the literature: the micro-interdigital electrode impedance biosensor has the advantages of high sensitivity, high response speed, low detection limit, moderate cost and convenience in carrying. However, it does not analyze the sensor structure design for impedance accuracy.
From this, it is known that the portable sensor is an important tool for studying the morphology and function of cells, and has a very important meaning in pharmacology. However, many of the conventional portable sensors have problems such as damage, large electrode contact resistance, and high generated noise, and the accuracy of impedance detection is seriously affected.
Therefore, there is a need to improve the prior art to overcome the deficiencies of the prior art.
Disclosure of Invention
The present application is directed to solve the above problems of the prior art and to provide a portable sensor for cell detection, which can greatly improve the detection accuracy.
It is another object of the present application to provide a method of assembling a detachable portable sensor for cell detection.
It is still another object of the present application to provide a cell measurement method.
It is yet another object of the present application to provide a method of designing a detachable portable sensor for cell detection.
The technical scheme of the application is as follows:
a detachable portable sensor for cell detection comprises a main body structure, an SMA connector electrode;
wherein the body structure comprises: an upper structure, a lower structure;
the upper structure comprises an upper PCB and an upper cover which are stacked from top to bottom; the upper PCB is provided with X groups of SMA joint holes and through holes, the upper cover is provided with an accommodating cavity, and the through holes arranged on the upper PCB correspond to the accommodating cavity arranged on the upper cover;
the lower structure includes: a sealing gasket, a lower PCB and a base; a concave space (step shape) is arranged in the middle of the base, the lower PCB and the sealing gasket are both arranged in the concave space of the base, and the sealing gasket is arranged at the upper part of the lower PCB; the lower PCB is provided with 1 group of SMA joint holes;
a circumferential electrode is arranged in the accommodating cavity;
the bottom of the accommodating cavity is provided with an opening;
x holes are formed in the sealing gasket, X gold immersion electrodes are also formed on the lower PCB, and X represents any natural number; the hole area of the sealing gasket is smaller than that of the gold immersion electrode; the sealing gasket can be replaced according to the requirement, the aperture of different sealing gaskets is different, and the area of the actual access contact area of the gold immersion electrode on the PCB is controlled by replacing the sealing gasket with different aperture, so that the electric field intensity is controlled to improve the sensitivity of cell detection;
SMA joint electrodes are arranged in each group of SMA joint holes of the upper PCB and 1 group of SMA joint holes on the lower PCB.
Furthermore, X pieces of gold immersion electrodes are in circuit communication.
Further, lower PCB board is T type shape, includes: the body and the cantilever section, the cantilever section is protruded out of the upper cover; and an SMA joint hole is arranged on the cantilever section.
Furthermore, the quantity that holds the chamber is a plurality of, all is provided with circumference electrode in every holds the chamber, go up the group number in SMA joint hole that sets up on the PCB board, the quantity of SMA joint electrode all with hold the quantity in chamber the same, every circumference electrode all with SMA connection electrode one-to-one intercommunication.
Furthermore, the number of each group of SMA joint holes is four, and the four joint electrode mounting holes are distributed in an array manner.
Further, the body is being provided with four heavy gold electrodes, the quantity of cylinder inspection hole is four, and four cylinder inspection holes adopt array distribution, the quantity of circumference electrode is four, and four circumference electrodes adopt array distribution.
Further, corresponding bolt holes are formed in the upper PCB, the upper cover and the base, and the upper structure and the lower structure are fixed through bolt-nut assemblies.
Further, the number of the bolt holes is six.
A method of assembling a detachable portable sensor for cell detection, comprising the steps of:
firstly, a lower PCB and a sealing gasket are sequentially arranged in a concave space of a base;
secondly, the upper PCB, the upper cover and the base are connected through a bolt-nut assembly;
finally, the SMA joint electrodes are installed on the upper PCB (as shown in fig. 1, the upper PCB is installed with four SMA joint electrodes) and the lower PCB (as shown in fig. 1, the upper PCB is installed with 1 SMA joint electrode).
A method for assembling a detachable portable sensor for cell detection, before placing a cylindrical detection hole in an upper cover, determining the hole size of a sealing gasket:
in the formula: d represents: the diameter of the sealing gasket is large; l represents: the distance between the long PCB and the lower PCB; a iscRepresents: cell diameter; ω represents the sensitivity, and its magnitude is set by itself, for example, 50% to 100%.
A cell measurement method adopts the detachable portable sensor for cell detection to carry out measurement; the cell model is put in a cylindrical detection hole in an upper cover after penetrating through an upper PCB, so that the 'SMA connector-immersion gold electrode 13-background liquid in a containing cavity-circumferential electrode 9-SMA connector of the upper PCB' mounted on a lower PCB 'is communicated', and measurement is carried out:
connecting the SMA joint electrode of the lower PCB with one end of the measuring equipment, and connecting the SMA joint electrode installed on the upper PCB with the other end of the measuring equipment; the impedance measurement of the object to be measured (i.e., the cell) is accomplished through the electrical signal path. (the sensor is suitable for impedance measurement by a two-electrode method).
The beneficial effect of this application lies in:
firstly, the structural design of the application is as follows: "Signal detection" is a basic design: the upper PCB is provided with a plurality of SMA joint electrodes, the containing cavity is provided with circumferential electrodes, then the gold immersion electrodes are connected with each other, and the gold immersion electrodes are communicated with the SMA joint electrodes of the lower PCB, so that a circuit signal path is formed (the containing cavity can be communicated by adding cell background liquid); meanwhile, the gold immersion electrodes of the lower PCB are communicated with each other, and only 1 SMA joint electrode needs to be arranged on the lower PCB.
Secondly, the structural design of this application, the hole design of seal gasket is one of the key point. For the hole design of the sealing gasket, the recognition is that: the size of the access detection area of the gold immersion electrode can be controlled by changing the sealing gasket, so that the electric field intensity is controlled to improve the sensitivity of cell detection. Sensitivity and chip cost are mutually limiting: on the one hand, for the user, the higher the sensitivity, the better the required pore size, which is why the microfluidic chip is used for cell detection; on the other hand, too small a hole will result in too high a chip manufacturing cost and a reduction in the reusability of the chip. The present application addresses this problem, using modified "microelectrodes" to achieve high sensitivity.
Thirdly, the detection area of the electrode which is actually connected is modified (the gasket is made of PDMMS and belongs to insulating materials; holes are punched on the gasket through the hole puncher with different diameters during preparation, so that holes with different diameters are reserved to ensure that the current is communicated with the circumferential electrode), and the focusing of an electric field is realized to improve the detection sensitivity.
The resistivity of the cell detection system is calculated by the following formula:
where rhomix，ρc，ρmRespectively representing the resistivity of a measuring system, a cell and a background liquid;
the volume fraction is expressed.
The sensitivity of the sensor can be obtained from the equations (3), (4), (5):
in the formula: rmixRepresents: measuring the mixed impedance of the system (a in equation 3)cR can be determined when it is 0). Δ R represents: the difference in impedance between the measured object and the non-measured object in the system is measured (the measured object is a cell). D represents: the diameter of the sensor actually inserted into the measurement increases the sensitivity of cell detection as D decreases. l represents: the distance between the upper and lower PCB boards. a iscRepresents: cell diameter. ω characterizes the magnitude of the sensitivity. From the above analysis, it can be seen that: this application can reach the area of revising the actual access and measuring and improve the purpose that detects the zero degree through the hole of revising seal gasket.
Thirdly, the flow rate of the mixed gas is increased.
Drawings
The present application will be described in further detail with reference to the following examples, which are not intended to limit the scope of the present application.
Fig. 1 is a perspective view of a portable sensor according to embodiment 1.
Fig. 2 is a sectional view of a perspective view of the portable sensor of embodiment 1.
Fig. 3 is a perspective view of the PCB board of the portable sensor of embodiment 1.
Fig. 4 is a perspective view of the upper cover of the portable sensor according to embodiment 1.
Fig. 5 is a perspective view of a portable sensor variable shim according to embodiment 1.
Fig. 6 is a perspective view of a lower PCB of the portable sensor of embodiment 1.
Fig. 7 is a three-dimensional schematic view of a portable sensor base according to embodiment 1.
The reference numerals in fig. 1-7 are illustrated as follows: SMA joint electrode 1, upper PCB 2, upper cover 3, sealing gasket 4, lower PCB 5, base 6, bolt-nut assembly 7, cylinder detection hole 8, circumferential electrode 9, joint electrode mounting hole 10, bolt hole 11, hole 12, gold immersion electrode 13, cell model 14, first mounting space 15, second mounting space 16.
Detailed Description
The first embodiment is as follows: detachable portable sensor for cell detection。
The structural design of the sensor of the first embodiment is shown in table 1.
TABLE 1 structural design of the sensor
The fitting design of the above components is as follows:
1) positional relationship between the upper PCB 2 and the upper cover 3: the upper PCB 2 is above the upper cover 3.
2) The position relation of the perforation and the containing cavity is as follows: the through hole arranged on the upper PCB board corresponds to the accommodating cavity arranged on the upper cover.
3) The position relation of the lower PCB 5, the sealing gasket 4 and the base 6 is as follows: a first mounting space 15 of the sealing gasket 4 and a second mounting space 16 of the lower PCB 5 are arranged in the middle of the base 6; the first installation space 15 communicates with the second installation space 16; the lower PCB 5 is disposed in the second mounting space 16, a gasket is disposed in the first mounting space 15, and the gasket is disposed at an upper portion of the lower PCB.
4) The mounting of the whole structure is fixed: corresponding bolt holes 11 are arranged on the upper PCB 2, the upper cover 3 and the base 6, and the upper structure and the lower structure are fixed through bolt-nut assemblies 7.
5)4 circumferential electrodes 9 are designed for the 4 cavities, and 4 SMA joint electrodes are needed at the moment. All the gold immersion electrodes 13(4 gold immersion electrodes 13 are communicated) share one SMA electrode joint. During actual measurement, one end of a measurement terminal of the measurement equipment is required to be connected with the common end, and one SMA connector of the upper PCB is arbitrarily selected at the other end, wherein a chamber corresponding to the selected SMA connector of the upper PCB is a chamber determined by measurement.
6) The sensor can be applied to the detection of single cells and cell suspensions.
The detachable portable sensor for cell detection of the present application adopts the following steps when assembled:
firstly, a lower PCB 5 and a sealing gasket 4 are sequentially arranged in a concave space of a base;
secondly, the upper PCB 2, the upper cover 3 and the base 6 are connected through a bolt-nut assembly 7;
finally, the SMA connector electrodes 1 are mounted on the upper PCB (four are mounted on the upper PCB as shown in fig. 1) and the lower PCB (1 is mounted on the upper PCB as shown in fig. 1) for detection.
Example two: design of sealing gasket。
In the first embodiment, the gasket seal is disclosed, but the function and selection strategy are not illustrated. The second embodiment aims to solve the above problems.
Structural design:
4 holes are arranged on the sealing gasket, and 4 gold immersion electrodes 13 with fixed areas are arranged on the lower PCB 5;
the positions of the 4 holes of the sealing gasket, the 4 gold-sinking electrodes 13 with fixed areas arranged on the lower PCB 5 and the accommodating cavity arranged on the upper cover 3 correspond to each other; the bottom of the containing cavity is provided with a small hole (the hole-the gold-depositing electrode can be increased or decreased according to the requirement, and the number and the position of the gold-depositing electrode 13 must correspond to the number and the position of the containing cavity).
The principle is as follows:
1) four gold-plated electrodes 13 are provided on the body of the lower PCB 5, and gold-plating treatment is performed on the electrodes, mainly considering the influence of reducing an electric double layer (the electric double layer refers to an ionic layer formed on the surface of the electrode by interfacial polarization between the electrode and a background liquid, and the ionic layer is similar to a parallel plate capacitor) due to low activity and excellent conductivity of gold.
2) The hole area of the sealing gasket is smaller than the area of the gold immersion electrode, so that the size of the area of the gold immersion electrode 13 used in actual measurement can be controlled, and cells with different volumes can be measured.
3) The hole is used for ensuring that the circumferential electrode 9 is communicated with the lower PCB 5 (the sealing gasket can be replaced according to needs, different sealing gasket apertures are different), and the area size of the actual contact area of the gold-deposited electrode on the PCB 5 is controlled by replacing the sealing gaskets with different apertures, so that the electric field intensity is controlled to improve the sensitivity of cell detection.
In the formula:
ρmrespectively representing the resistivity of the background liquid of the measuring system;
r represents: measuring the impedance of the system in the absence of cells;
Δ R represents: the difference in impedance between the measured object and the non-measured object in the system is measured (the measured object is a cell).
D represents: the diameter (namely the size of the hole of the sealing gasket) of the sensor actually connected in measurement can increase the sensitivity of cell detection when D is reduced;
l represents: the distance between the long PCB and the lower PCB.
acRepresents: cell diameter.
ω characterizes the magnitude of the sensitivity.
The detachable portable sensor for cell detection of the application adopts the following steps when in use:
1) selecting a proper gasket;
2) assembling the sensor;
3) after the sensor is installed, the cell model 14 is put in the cylindrical detection hole 8 in the upper cover 3 through the upper PCB 2 (after the sensor assembly and the cell placement are completed, one end of four kelvin clamps on the impedance analyzer is connected with the bottom electrode, namely the SMA interface 10, and the other end is connected with any SMA connector on the upper PCB).
In the first and second embodiments, it should be further explained that:
the number of each group of joint electrode mounting holes 10 is four, and the four joint electrode mounting holes 10 are distributed in an array manner.
The number of the bolt holes is six.
The number of the cylindrical detection holes 8 is four, and the four cylindrical detection holes 8 are distributed in an array manner.
The number of the circumferential electrodes 9 is four, and the four circumferential electrodes 9 are distributed in an array manner.
The above-mentioned embodiments are merely preferred embodiments of the present application, which are not intended to limit the present application in any way, and it will be understood by those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the present application.
Claims (10)
1. A detachable portable sensor for cell detection is characterized by comprising a main body structure and SMA joint electrodes;
wherein the body structure comprises: an upper structure, a lower structure; the upper structure comprises an upper PCB and an upper cover which are stacked from top to bottom; the upper PCB is provided with X groups of SMA joint holes and through holes, the upper cover is provided with an accommodating cavity, and the through holes arranged on the upper PCB correspond to the accommodating cavity arranged on the upper cover; the lower structure includes: a sealing gasket, a lower PCB and a base; a concave space is arranged at the middle position of the base, the lower PCB and the sealing gasket are both arranged in the concave space of the base, and the sealing gasket is arranged at the upper part of the lower PCB; the lower PCB is provided with 1 group of SMA joint holes;
a circumferential electrode is arranged in the accommodating cavity;
the bottom of the accommodating cavity is provided with an opening;
x holes are formed in the sealing gasket, X gold immersion electrodes are also formed on the lower PCB, and X represents any natural number;
SMA joint electrodes are arranged in each group of SMA joint holes of the upper PCB and 1 group of SMA joint holes on the lower PCB.
2. The detachable portable sensor for cell detection according to claim 1, wherein X gold immersion electrodes are in circuit communication; the hole area of the sealing gasket is smaller than that of the gold-sinking electrode.
3. The detachable portable sensor for cell detection according to claim 2, wherein the lower PCB is T-shaped, comprising: the body and the cantilever section, the cantilever section is protruded out of the upper cover; and an SMA joint hole is arranged on the cantilever section.
4. The detachable portable sensor for cell detection according to claim 2, wherein the number of the accommodating cavities is plural, each accommodating cavity is provided with a circumferential electrode, the number of the groups of the SMA connector holes and the number of the SMA connector electrodes provided on the upper PCB are the same as the number of the accommodating cavities, and each circumferential electrode is in one-to-one communication with the SMA connecting electrodes.
5. The detachable portable sensor for cell detection according to claim 2, wherein each set of SMA connector holes is four, and four connector electrode mounting holes are distributed in an array.
6. The detachable portable sensor for cell detection according to any one of claims 1 to 5, wherein corresponding bolt holes are provided on the upper PCB, the upper cover and the base, and the upper structure and the lower structure are fixed by a bolt-nut assembly.
7. The detachable portable sensor for cell detection according to any one of claims 1 to 5, wherein the body is provided with four gold immersion electrodes, the number of the cylindrical detection holes is four, the four cylindrical detection holes are distributed in an array, the number of the circumferential electrodes is four, and the four circumferential electrodes are distributed in an array.
8. A method of assembling a detachable portable sensor for cell assay according to claim 6, comprising the steps of:
firstly, a lower PCB and a sealing gasket are sequentially arranged in a concave space of a base;
secondly, the upper PCB, the upper cover and the base are connected through a bolt-nut assembly;
and finally, mounting SMA joint electrodes on the upper PCB and the lower PCB.
9. A cell measuring method using the detachable portable sensor for cell detection according to any one of claims 1 to 7;
the method is characterized in that:
the cell model is arranged in a cylindrical detection hole in an upper cover after penetrating through an upper PCB, so that the 'SMA joint-immersion gold electrode-background liquid in a containing cavity-circumferential electrode-SMA joint of the upper PCB' mounted on a lower PCB 'are communicated', and measurement is carried out:
connecting the SMA joint electrode of the lower PCB with one end of the measuring equipment, and connecting the SMA joint electrode installed on the upper PCB with the other end of the measuring equipment; and completing the impedance measurement of the measured object through the electric signal path.
10. A method of designing a detachable portable sensor for cell detection according to claim 1, wherein the size of the hole of the gasket is estimated by the following formula:
in the formula:
d represents: the diameter of the sealing gasket is large;
l represents: the distance between the long PCB and the lower PCB;
acrepresents: cell diameter;
ω represents the magnitude of the sensitivity, which is self-setting.
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USRE37977E1 (en) * | 1997-12-25 | 2003-02-04 | Matsushita Electric Industrial Co., Ltd. | Cell potential measuring electrode and measuring apparatus using the same |
| CN1681937A (en) * | 2002-07-20 | 2005-10-12 | 美国艾森生物科学公司 | Apparatuses and method based impedance for analyzing cells and granules |
| US20060057771A1 (en) * | 2004-03-10 | 2006-03-16 | Kovacs Gregory T | Low cost fabrication of microelectrode arrays for cell-based biosensors and drug discovery methods |
| US20080227664A1 (en) * | 2007-03-16 | 2008-09-18 | Canon Kabushiki Kaisha | Cell array structural body and cell array |
| CN101614729A (en) * | 2008-06-27 | 2009-12-30 | 博奥生物有限公司 | The microelectrode array device and the isolated plant that are used for cell manipulation and electrophysiologicalsignal signal detection |
| CN102304475A (en) * | 2011-09-08 | 2012-01-04 | 岭南大学校产学协力团 | Cell electrofusion chip device based on surface microelectrode array and deformation membrane structure |
| CN107475072A (en) * | 2017-08-24 | 2017-12-15 | 浙江理工大学 | Cell dynamic characteristic monitoring system and method based on three-dimensional grapheme Interfacial electrode |
| CN109580731A (en) * | 2019-01-15 | 2019-04-05 | 安徽师范大学 | The preparation method and the application in detection Polychlorinated biphenyls of DNA micro-capsule and gold electrode-DNA dendritic macromole sensor |
| US20190162688A1 (en) * | 2017-10-20 | 2019-05-30 | University Of Central Florida Research Foundation, Inc. | Interdigitated electrodes for in vitro analysis of cells |
| CN110082414A (en) * | 2019-05-30 | 2019-08-02 | 山西大学 | Aptamer-ferronickel cyanogen nanoparticle-RGO electrode preparation and application |
| US20210095235A1 (en) * | 2019-09-30 | 2021-04-01 | John Collins | Fluidic array systems and testing for cells, organoids, and organ cultures |
-
2021
- 2021-12-14 CN CN202111524332.6A patent/CN114264590B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USRE37977E1 (en) * | 1997-12-25 | 2003-02-04 | Matsushita Electric Industrial Co., Ltd. | Cell potential measuring electrode and measuring apparatus using the same |
| CN1681937A (en) * | 2002-07-20 | 2005-10-12 | 美国艾森生物科学公司 | Apparatuses and method based impedance for analyzing cells and granules |
| US20060057771A1 (en) * | 2004-03-10 | 2006-03-16 | Kovacs Gregory T | Low cost fabrication of microelectrode arrays for cell-based biosensors and drug discovery methods |
| US20080227664A1 (en) * | 2007-03-16 | 2008-09-18 | Canon Kabushiki Kaisha | Cell array structural body and cell array |
| CN101614729A (en) * | 2008-06-27 | 2009-12-30 | 博奥生物有限公司 | The microelectrode array device and the isolated plant that are used for cell manipulation and electrophysiologicalsignal signal detection |
| CN102304475A (en) * | 2011-09-08 | 2012-01-04 | 岭南大学校产学协力团 | Cell electrofusion chip device based on surface microelectrode array and deformation membrane structure |
| CN107475072A (en) * | 2017-08-24 | 2017-12-15 | 浙江理工大学 | Cell dynamic characteristic monitoring system and method based on three-dimensional grapheme Interfacial electrode |
| US20190162688A1 (en) * | 2017-10-20 | 2019-05-30 | University Of Central Florida Research Foundation, Inc. | Interdigitated electrodes for in vitro analysis of cells |
| CN109580731A (en) * | 2019-01-15 | 2019-04-05 | 安徽师范大学 | The preparation method and the application in detection Polychlorinated biphenyls of DNA micro-capsule and gold electrode-DNA dendritic macromole sensor |
| CN110082414A (en) * | 2019-05-30 | 2019-08-02 | 山西大学 | Aptamer-ferronickel cyanogen nanoparticle-RGO electrode preparation and application |
| US20210095235A1 (en) * | 2019-09-30 | 2021-04-01 | John Collins | Fluidic array systems and testing for cells, organoids, and organ cultures |
Non-Patent Citations (1)
| Title |
|---|
| 徐莹 等: "基于MEMS技术的微电极阵列细胞传感器", 自然科学进展, vol. 17, no. 09, pages 1265 - 1272 * |
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