CN1142611A - 唾液酸检测试剂 - Google Patents
唾液酸检测试剂 Download PDFInfo
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- CN1142611A CN1142611A CN 95110900 CN95110900A CN1142611A CN 1142611 A CN1142611 A CN 1142611A CN 95110900 CN95110900 CN 95110900 CN 95110900 A CN95110900 A CN 95110900A CN 1142611 A CN1142611 A CN 1142611A
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Abstract
一种唾液酸检测试剂，包括标准液、反应液和多元萃取液。反应液、以地衣酚、盐酸、硫酸铜、氯化铬或氯化铁、蒸馏水按一定比例配制而成；多元萃取液，以丁醇、乙酸丁酯、戊醇或异戊醇、表面活性剂按一定比例配制而成。用于测定体液中唾液酸含量，具有灵敏度高，选择性、准确度和精密度好，操作简单快速等优点，可为多种恶性肿瘤的早期诊断、疗效观察及病情追踪提供科学实用、简便可靠的临床检测手段。
Description
本发明涉及一种临床检测试剂，特别是一种唾液酸检测试剂。
现有的唾液酸检测试剂，因其选择性差，尤其是共存的糖类及维生素C等组分的严重干扰，所以测定的准确度和精密度不高，难以达到临床检测的要求。
本发明的目的是为了克服上述不足之处，而提供一种灵敏度高，共存组分的干扰小，检测的准确度和精密度好的唾液酸检测试剂。
本发明的技术解决方案是这样的：唾液酸检测试剂包括唾液酸标准液，其特征是它还分别配制有反应液和多元萃取液。反应液，按质量/体积比，以地衣酚0.1％-1％、盐酸5％-25％、硫酸铜0.02％-0.05％、氯化铬或氯化铁0.01％-0.05％、蒸馏水74％-94％混合，溶解稀降而制取。多元萃取液，按容积比，以正丁醇5％-15％、乙酸丁酯75％-89％、正戊醇或异戊醇5％-10％、表面活性剂0.1％-0.5％混合均匀而制取。
实施例：
1、配制反应液：以制备100ml为例，取地衣酚0.5克、浓盐酸30ml、硫酸铜0.05克、氯化铬0.03克，用蒸馏水溶解稀释至100ml即成。
2、配制多元萃取液：以制备100ml为例，取正丁醇8ml、正戊醇15ml、乙酸丁酯76.5ml、乳化剂op0.5ml混合均匀成透明无色溶液即成。
将反应液、多元萃取液和唾液酸标准液分别按使用比例装瓶密封，包装成一盒，置于2℃-8℃环境下冷藏待用。
本发明由于采用以上配方，制取的试剂用于测定体液中唾液酸含量，灵敏度高，共存组分的干扰小，检测结果的准确度和精密度都好，且操作简单、快速，完全达到临床检测的要求，为肺癌、肝癌、胃癌等多种肿瘤的早期诊断或辅助诊断，疗效观察及病情追踪提供了科学实用、灵敏准确、简便可靠的检测手段。
Claims (1)
1、一种唾液酸检测试剂，包括唾液酸标准液，其特征是它还分别配制有反应液和多元萃取液，反应液，按质量/体积比，以地衣酚0.1％-1％、盐酸5％-25％、硫酸铜0.02％-0.05％、氯化铬或氯化铁0.01％-0.05％、蒸馏水74％-94％混合，溶解稀释而制取；多元萃取液，按容积比，以正丁醇5％-15％、乙酸丁酯75％-89％、正戊醇或异戊醇5％-10％、表面活性剂0.1％-0.5％混合均匀而制取。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95110900 CN1142611A (zh) | 1995-08-04 | 1995-08-04 | 唾液酸检测试剂 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95110900 CN1142611A (zh) | 1995-08-04 | 1995-08-04 | 唾液酸检测试剂 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1142611A true CN1142611A (zh) | 1997-02-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95110900 Pending CN1142611A (zh) | 1995-08-04 | 1995-08-04 | 唾液酸检测试剂 |
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| CN (1) | CN1142611A (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327226C (zh) * | 2002-09-09 | 2007-07-18 | 株式会社Gc | 唾液预处理试剂盒及唾液预处理方法 |
| CN102072953A (zh) * | 2010-12-30 | 2011-05-25 | 北京九强生物技术有限公司 | 一种稳定的酶法检测唾液酸的方法及试剂盒 |
| CN111443078A (zh) * | 2020-04-16 | 2020-07-24 | 斯瑞尔环境科技股份有限公司 | 一种同时检测氯化亚铁中痕量As、Pb、Cd、Zn、Cr元素含量的方法 |
-
1995
- 1995-08-04 CN CN 95110900 patent/CN1142611A/zh active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327226C (zh) * | 2002-09-09 | 2007-07-18 | 株式会社Gc | 唾液预处理试剂盒及唾液预处理方法 |
| CN102072953A (zh) * | 2010-12-30 | 2011-05-25 | 北京九强生物技术有限公司 | 一种稳定的酶法检测唾液酸的方法及试剂盒 |
| CN102072953B (zh) * | 2010-12-30 | 2016-06-01 | 北京九强生物技术股份有限公司 | 一种稳定的酶法检测唾液酸的方法及试剂盒 |
| CN111443078A (zh) * | 2020-04-16 | 2020-07-24 | 斯瑞尔环境科技股份有限公司 | 一种同时检测氯化亚铁中痕量As、Pb、Cd、Zn、Cr元素含量的方法 |
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