CN1142610A - Method for analyzing non-covalent effect between biological composition - Google Patents
Method for analyzing non-covalent effect between biological composition Download PDFInfo
- Publication number
- CN1142610A CN1142610A CN 95107971 CN95107971A CN1142610A CN 1142610 A CN1142610 A CN 1142610A CN 95107971 CN95107971 CN 95107971 CN 95107971 A CN95107971 A CN 95107971A CN 1142610 A CN1142610 A CN 1142610A
- Authority
- CN
- China
- Prior art keywords
- electrophoresis
- biological
- radioactive
- biotin
- mark
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
A method for analyzing noncovalent action between biologic components includes such steps as use of biotin reagent and radioactive isotop to label different components, putting labeled components in liquid for reacting on each other, adding avidin or like streptavidin covalently crosslinked to solid supporter, mixing and waiting for a certain time, washing solid substance with proper buffering liquid, collecting solid substance, preparing gel sample for electrophoresis, doing electrophoresis, radioactive self-developing of gel after electrophoresis, and comparation of self-developed results to determine which isotop labeled moleculae have special mutual action on biotin labeled ones.
Description
What the present invention relates to is a kind of method of analyzing non-covalent effect between biological composition, particularly unites use, the different biological components of mark with biotin and radioactive isotope, to study the method for biological intermolecular interaction.
In the biological study field, we often need to determine will produce which type of interaction between the biological components of some characteristic the unknown.Known research method is still undesirable aspect accuracy.
Known in the test organisms molecular engineering people usually adopt a kind of labelled with radioisotope method that certain biological components is carried out spike to detect.The technology of another test organisms molecule is the derivant mark biomolecule with biotin (biotin), the detection technique that biological components to be detected is separated.
The objective of the invention is radioisotope method in the detection of biological molecular engineering and biotin method are united use in the analyzing non-covalent effect between biological composition checkout procedure, thereby a kind of method that can fully not interact and analyze between two groups of biological components of understanding as yet its biological property exactly is provided.
The method that the present invention proposes is made of following steps:
1, with biotinylation reagent and the different biological components of radioactive isotope difference mark;
2, these the two kinds biological components that mark is good are placed liquid phase, it is interacted under certain conditions;
3, add antibiotin (avidin) or other analog streptavidin of covalent cross-linking then, mix the back incubation after the regular hour, fully wash solid formation with suitable damping fluid again in solid support;
4, collect solid formation, preparative gel electrophoresis sample, thereby make gel electrophoresis;
5, do radioactive automatic developing with the gel behind the above-mentioned electrophoresis;
6, the result to the autography of the biological sample handled through above-mentioned steps compares analysis, with the molecule of definite those labelled with radioisotope with there being specific interaction between the biotin labeled molecule.
Specifically, be that B. carries out mark with biotin chemistry reagent on component A if one of them biological components is A, another biological components, and on B component, carry out mark with radioactive isotope.In liquid phase, make the two-way interaction then.If the two can interact, then will produce a product A+B.In this product, promptly contain biotin reagent and also contain isotope reagent.So when adding covalent cross-linking in the antibiotin of solid support, above-mentioned product then will generate solid formation with the antibiotin effect because of it contains biotin, and this solid formation is carried out can producing gel behind the electrophoresis.Owing to this gel is derived by A+B, thereby wherein also contain isotope.Whether therefore can detect it at an easy rate with radioactive automatic developing exists.Can analyze A and B thus and whether produce noncovalent interaction.
By above-mentioned narration as can be seen, adopt this method can utilize the advantage of the easily separated and easy purifying of the biomolecule of good advantage of the high sensitivity of labelled with radioisotope method and specificity and biotinylation mark simultaneously, thereby the method for interaction property between a kind of component of test organisms exactly is provided.
Claims (1)
1, a kind of method of analyzing non-covalent effect between biological composition is characterized in that this method comprises the steps:
1., with biotinylation reagent and the different biological components of radioactive isotope difference mark;
2., these the two kinds biological components that mark is good are placed liquid phase, it is interacted under certain conditions;
3., then add antibiotin (avidin) or other analog streptavidin of covalent cross-linking, mix the back incubation after the regular hour, fully wash solid formation with suitable damping fluid again in solid support;
4., collect solid formation, preparative gel electrophoresis sample, thereby make gel electrophoresis;
5., do radioactive automatic developing with the gel behind the above-mentioned electrophoresis;
6., the result of the autography of the biological sample handled through above-mentioned steps is compared analysis, with the molecule of definite those labelled with radioisotope with there being specific interaction between the biotin labeled molecule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95107971 CN1142610A (en) | 1995-08-08 | 1995-08-08 | Method for analyzing non-covalent effect between biological composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95107971 CN1142610A (en) | 1995-08-08 | 1995-08-08 | Method for analyzing non-covalent effect between biological composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1142610A true CN1142610A (en) | 1997-02-12 |
Family
ID=5076556
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95107971 Pending CN1142610A (en) | 1995-08-08 | 1995-08-08 | Method for analyzing non-covalent effect between biological composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1142610A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1052510A4 (en) * | 1998-11-06 | 2002-09-25 | Iatron Lab | Novel complexes containing crosslinked avidin, analytical method with the use of crosslinked avidin and analytical reagents and kits |
-
1995
- 1995-08-08 CN CN 95107971 patent/CN1142610A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1052510A4 (en) * | 1998-11-06 | 2002-09-25 | Iatron Lab | Novel complexes containing crosslinked avidin, analytical method with the use of crosslinked avidin and analytical reagents and kits |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Clark et al. | Enzyme-linked immunosorbent assay (ELISA): Theoretical and practical aspects | |
| FI93781B (en) | Biospecific multiparametric method of determination | |
| CN100420947C (en) | Method for quantitative determination of specific analyte with single trapping agent and reagent kit therefor | |
| Dill et al. | Detection of salmonella in poultry using a silicon chip-based biosensor | |
| DE69521253D1 (en) | QUICK DETECTION OF ANALYTES WITH RECEPTORS IMMOBILIZED ON SOLUBLE SUBMICRON PARTICLES | |
| JPS5951353A (en) | Unhomogeneous system immunity analysis method and reagent for analyzing hapten or antigen in liquid | |
| Badyal et al. | A simple method for the quantitative analysis of resin bound thiol groups | |
| DE60023539D1 (en) | IMMUNOLOGICAL DETECTION OF RNA: DNA HYBRID ON MICROARRAYS | |
| CN101144815A (en) | Preparation method of liquid phase protein chip | |
| Märtlbauer et al. | Immunochemical detection of antibiotics and sulfonamides | |
| CA1140045A (en) | Carcinoembryonic antigen determination | |
| ATE169738T1 (en) | METHOD FOR DETECTING A SPECIFIC COMPONENT IN A SAMPLE AND REAGENT THEREOF. | |
| KR870009031A (en) | Solution Phase Single Hybrid Assay for Polynucleotide Sequence Detection | |
| CN110426515A (en) | A kind of time-resolved fluoroimmunoassay chromatographic technique detects kit and its application of dirty underwater trace drugs | |
| ATE397215T1 (en) | DISRUPTION OF IMMUNOASSAYS BY SUBSTANCES DERIVED FROM THE FRAMEWORK REGIONS OF ANTIBODIES | |
| CN1142610A (en) | Method for analyzing non-covalent effect between biological composition | |
| CA2642575A1 (en) | Quantitation of cellular dna and cell numbers using element labeling | |
| Meyer | Automated stand-alone flow injection immunoanalysis system for the determination of cephalexin in milk | |
| ES462365A1 (en) | Method and apparatus for the detection of a specific binding protein | |
| US20070148784A1 (en) | Novel methods for determining the negative control value for multi-analyte assays | |
| DK528286D0 (en) | PROCEDURE FOR DETERMINING AN IMMUNOLOGICAL BONDABLE ANALYST | |
| USRE32696E (en) | Enzymatic immunological method for determination of antigens and antibodies | |
| EP0649530A4 (en) | CHEMILUMINESCENT ASSAY FOR dsDNA ANTIBODIES. | |
| Englebienne et al. | Water-soluble conductive polymer homogeneous immunoassay (SOPHIA) A novel immunoassay capable of automation | |
| JPH01221665A (en) | Emulation solid phase immunoassay method for detecting single epitope analysis matter and kit therefor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |