CN114236039A - Automatic titrator used together with Zeta potentiometer and using method thereof - Google Patents
Automatic titrator used together with Zeta potentiometer and using method thereof Download PDFInfo
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- CN114236039A CN114236039A CN202111577923.XA CN202111577923A CN114236039A CN 114236039 A CN114236039 A CN 114236039A CN 202111577923 A CN202111577923 A CN 202111577923A CN 114236039 A CN114236039 A CN 114236039A
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- 238000000034 method Methods 0.000 title claims description 31
- 238000004448 titration Methods 0.000 claims abstract description 233
- 239000000523 sample Substances 0.000 claims abstract description 99
- 238000003756 stirring Methods 0.000 claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 239000012488 sample solution Substances 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims description 31
- 230000002572 peristaltic effect Effects 0.000 claims description 24
- 210000004907 gland Anatomy 0.000 claims description 14
- 238000001514 detection method Methods 0.000 claims description 8
- 230000007613 environmental effect Effects 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 6
- 239000000654 additive Substances 0.000 claims description 6
- 239000002585 base Substances 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 238000010561 standard procedure Methods 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 3
- 230000000977 initiatory effect Effects 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 150000007513 acids Chemical class 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 63
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- 238000010586 diagram Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
- G01N31/162—Determining the equivalent point by means of a discontinuity
- G01N31/164—Determining the equivalent point by means of a discontinuity by electrical or electrochemical means
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Abstract
The invention discloses an automatic titrator used together with a Zeta potentiometer, wherein a sample to be detected is placed in a sample mixing pool, titrating liquids are respectively placed in No. 1-3 titrating pools, titrating liquid information in the No. 1-3 titrating pools is input in software, the volume of the sample in the sample mixing pool is input, and the upper and lower limit ranges of the initial and ending solution environments of titration and the titration steps and the number of steps performed in the ranges are set. Reading the pH value of a current sample through a pH electrode, comparing the target pH value set in the first step, logically judging which titration solution from No. 1-3 is adopted for carrying out the titration in the step, adding the required amount of titration solution obtained through calculation into the sample solution in a sample mixing pool by a titration pump, uniformly mixing the solution and the titration solution through electromagnetic stirring, calculating and confirming the volume of the titration solution needing to be continuously added into the sample through a feedback signal of a pH electrode sensor, and carrying out the titration operation in a reciprocating circulating mode until the target pH value set in the first step is reached.
Description
Technical Field
The invention relates to the technical field of automatic titrators, in particular to an automatic titrator used together with a Zeta potentiometer and a using method thereof.
Background
The change of the solution environment such as pH, salinity and other factors can affect the particle size and Zeta potential of a particle suspension system, so that a great deal of titration and scanning test requirements for the solution environment exist in the process of testing the nanometer particle size and the Zeta potential, and the particle size and potential information of the system in a series of solution environments (such as pH value 1 to 12) can be obtained through a continuous titration test for a sample, so that accurate stability information of products can be obtained, and a good data base and a solution are provided for the optimization of a formula and a process.
The traditional titration operation is manually performed by an operator, the test is performed after the sample is titrated step by step, the test speed is low, the repeatability of the test result is poor, and the operation skill level of personnel is depended on. The accuracy is not high, and the detection efficiency is reduced.
Disclosure of Invention
The invention aims to provide an automatic titrator used together with a Zeta potentiometer and a using method thereof, and the specific scheme is as follows:
an automatic titrator used together with a Zeta potentiometer comprises a box body, an upper cover, a side cover, a sample mixing pool, a miniature electromagnetic stirring device, a No. 1 titration solution pool, a No. 2 titration solution pool, a No. 3 titration solution pool, a No. 1 titration pump, a No. 2 titration pump, a No. 3 titration pump, a peristaltic pump, a pH electrode, a pipeline a, an electromagnetic valve, an integrated circuit, a pipeline b, a titration solution pool support, a pH electrode clamp, a pipeline adapter, a threaded sleeve, a titration pump guide rail, a titration pump shunting block, a stepping motor gear rack group, a side cover support plate, a side cover support column, a base and a titration pump electromagnetic valve;
the pH electrode bracket is in an inverted L shape and is fixed on the base;
one end of the sample mixing pool is screwed on a pH electrode clamp through threads, a pH electrode is fixed on a pH electrode bracket through the pH electrode clamp, and the pH electrode extends into the sample mixing pool;
the other end of the sample mixing pool is suspended on the miniature electromagnetic stirring device;
the side cover supporting plate is fixed on the base, and a titration liquid pool pipeline bracket is arranged on the side surface of one end of the side cover supporting plate;
the pipeline adapter is arranged at the top end of the side cover supporting plate through a pipeline adapter support, the titration pump shunt block and the peristaltic pump are arranged at the upper end of the front side of the side cover supporting plate in parallel, the titration pump guide rail and the gear rack group of the stepping motor are arranged on the side cover supporting plate at the lower end of the titration pump shunt block in parallel, and the side cover support columns are fixed on the side cover supporting plate at two sides of the titration pump guide rail;
the titration pump electromagnetic valve is fixed on the titration pump shunting block, and the side cover is fixed on the side cover supporting plate through a side cover strut;
the integrated circuit interface bracket is arranged on the opposite side of the pH electrode bracket, the integrated circuit is arranged on the rear side of the side cover supporting plate and is fixed on the base through the integrated circuit interface bracket, the signal input end of the integrated circuit receives the instructions of the pH electrode and the control unit, and the output end outputs control signals to be connected to the miniature electromagnetic stirring device, the No. 1 titration pump, the No. 2 titration pump, the No. 3 titration pump and the peristaltic pump;
the side cover supporting plate, the integrated circuit interface bracket and the pH electrode bracket are fastened and fixed on the base by the box body;
the electromagnetic stirring device penetrates through the pH electrode support through an electromagnetic stirring support connecting rod and is connected with the box body;
the titrant pool support is fixed on the box body through a titrant pool support connecting rod;
the titrant pool support is provided with a No. 1 titrant pool, a No. 2 titrant pool and a No. 3 titrant pool;
the No. 1 titration liquid pool, the No. 2 titration liquid pool and the No. 3 titration liquid pool penetrate through the titration liquid pool pipeline bracket through the pipeline b and are correspondingly connected with the No. 1 titration pump, the No. 2 titration pump and the No. 3 titration pump;
the indicator light is fixed on the box body;
the upper cover is buckled at the upper ends of the box body and the side cover, and a pH electrode of the upper cover penetrates out of a mounting port arranged on the upper cover;
the pipeline a which passes through the pipeline adapter through the peristaltic pump and is connected with the electromagnetic valve penetrates out of the upper cover notch, and the pipeline a is used for a pipeline which is connected with the capillary electrode and used for sample circulation.
The preferred scheme of the automatic titrator used together with the Zeta potentiometer is that the miniature electromagnetic stirring device comprises an electromagnetic stirring device upper cover, an electromagnet upper gland, an electromagnet lower gland, an electromagnet, a direct current motor bracket and an electromagnetic stirring bracket;
the electromagnet is tightly pressed with the electromagnet lower gland through the electromagnet upper gland and is fixed on a direct current motor shaft;
the direct current motor is fixed on the direct current motor support and is fixed on the electromagnetic stirring support together with the electromagnetic stirring device upper cover.
The preferred scheme of the automatic titrator used together with the Zeta potentiometer is that the titration solutions are respectively placed in a No. 1 titration solution pool, a No. 2 titration solution pool and a No. 3 titration solution pool, and the titration solutions comprise but are not limited to acids, alkalis and salts.
The preferred scheme of the automatic titrator used together with the Zeta potentiometer is that the minimum titration volume of the titration solution is 1.68ul, and no upper limit requirement exists;
the pump flow of the peristaltic pump is adjustable within the range of 0-45 ml/min, and the peristaltic pump has the capacity of conveying a titrated sample into the capillary electrode and conveying a Zeta-potential-test-finished sample back to the mixing pool.
The preferred scheme of the automatic titrator used together with the Zeta potentiometer is that the stirring speed of the miniature electromagnetic stirring device is controllable within the range of 0-2000 r/min, and a sample and a titration solution are uniformly mixed by generating vortex through magnetons in an electric sample mixing pool.
A method of using an automatic titrator in conjunction with a Zeta potential meter, comprising the steps of: the method comprises the following steps: placing a sample to be detected in a sample mixing pool, setting titration test conditions at a software end, respectively placing titration solutions in a No. 1 titration pool, a No. 2 titration pool and a No. 3 titration pool, inputting information of the titration solutions in the No. 1 titration pool, the No. 2 titration pool and the No. 3 titration pool in software, including pH and salinity, inputting the volume of the sample in the sample mixing pool, and setting upper and lower limit ranges of initial and final solution environments for titration and titration steps and step numbers performed in the ranges;
step two: in the first step of titration, firstly reading the pH value of a current sample through a pH electrode, comparing the target pH value set in the first step, selecting a titration solution in a No. 1 titration cell, a No. 2 titration cell and a No. 3 titration cell to perform the titration in the first step through logic judgment, adding a required amount of the titration solution obtained through calculation into a sample solution in a sample mixing cell by a corresponding titration pump, uniformly mixing the solution and the titration solution through electromagnetic stirring, calculating and confirming the volume of the titration solution needing to be continuously added into the sample through a feedback signal of a pH electrode sensor, closing the electromagnetic stirring if the target pH value is reached, starting the electromagnetic valve, finishing the titration, and performing reciprocating circular titration operation until the target pH value set in the first step is reached if the target pH value is not reached;
step three: injecting a sample reaching a target pH value into a capillary electrode which is inserted into a Zeta potentiometer host in advance through a pipeline a connected with the capillary electrode by a peristaltic pump, closing a flow path through an electromagnetic valve, and carrying out Zeta potential detection on the sample in the Zeta potentiometer host;
step four: after the detection is finished, opening a flow path through an electromagnetic valve, conveying the sample from the capillary electrode to the sample mixing pool through a peristaltic pump, and then carrying out second-step titration and test operation;
step five: and circulating until the sample reaches the set termination solution environment.
The preferred scheme of the using method of the automatic titrator used together with the Zeta potentiometer is that the method for selecting the type of the titration solution in the pH titration process comprises the following steps:
1) reading the pH value of the current sample, and comparing the pH value with a target pH value;
2) respectively calculating the sample adding amount required by No. 1-3 titration solutions to reach the target pH value and sequencing;
3) if the lowest sample adding amount is more than 1.68ul, titrating by using the titration solution corresponding to the lowest sample adding amount;
4) if the lowest sample adding amount is less than 1.68ul, comparing whether the second lowest sample adding amount is less than 1.68 mu L, and if so, titrating by using a titration solution corresponding to the second lowest sample adding amount;
5) and 4) if not, titrating the titration solution corresponding to the highest sample adding amount.
The preferred scheme of the using method of the automatic titrator used together with the Zeta potentiometer is that a matched software system is compatible with a Zeta potentiometer software system, and before the test is started, the software system can be used for setting:
1) type of titration including, but not limited to, pH, salinity, and concentration of additives;
2) initiating and terminating a step interval within the solution environmental parameter range;
3) redundancy over a range of starting and ending solution environmental parameters;
4) defining each kind of titration liquid, including but not limited to acid, alkali, salt, additive;
5) the system has SOP capability, and is convenient for a user to make, store and call standard operation procedures.
The preferred scheme of the using method of the automatic titrator used together with the Zeta potentiometer is that a matched software system can be compatible with a Zeta potentiometer software system, and the software system can calculate according to the test type, the sensor feedback signal and the input parameter in the test process:
1) the type of the titration solution required to be added to achieve the target solution environment;
2) the volume of the titration solution required to reach the target solution environment;
3) and (3) adding the titrating solution to reach the target solution environment.
The preferred scheme of the using method of the automatic titrator used together with the Zeta potentiometer is that a matched software system of the automatic titrator is compatible with a Zeta potentiometer software system, and the software system can communicate with a control circuit of the automatic titrator and issue commands according to a calculation result obtained by a test type, a sensor feedback signal and an input parameter in the test process.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention can realize automatic titration test and sample introduction test, has the advantages of high speed, good repeatability, no dependence on operators, capability of measuring colored samples, turbid samples and samples without suitable indicators, and the like, can greatly improve the detection efficiency, and provides a powerful automatic means for scientific research and product quality inspection;
2. the invention has an intelligent testing process, can automatically measure by an instrument only by inputting parameters such as sample amount, standard solution concentration, pH initial value and the like, and has simple and rapid operation. The test data can be automatically recorded, counted and analyzed, so that the workload and operation errors of manually analyzing the original data are reduced, and the reliability and accuracy of the experimental data are improved.
3. The invention can be widely applied to precise electricians, electronics, instruments and meters and other products when being used together with a Zeta potentiometer, and is mainly applied to the research and application fields of medical treatment and health, biological pharmacy, agricultural scientific research, environmental protection and the like. The method effectively fills the blank of the technology in China and has important significance for increasing economic benefit and social benefit.
Drawings
FIG. 1 is a schematic block diagram of an automatic titrator in use with a Zeta potentiometer according to the present invention;
FIG. 2 is a left side view of the present invention;
FIG. 3 is a right side view of the present invention;
FIG. 4 is a left side view of the internal perspective structure of the present invention;
FIG. 5 is a right side view of the internal perspective structure of the present invention;
FIG. 6 is a schematic view of a micro electromagnetic stirring apparatus according to the present invention;
FIG. 7 is a flow chart of a first titration step of the present invention;
FIG. 8 is a logic flow diagram for selecting a titration solution during each titration step of the present invention.
Wherein, 1 is a sample mixing tank, 2 is a miniature electromagnetic stirring device, 3 is a No. 1 titration liquid tank, 4 is a No. 2 titration liquid tank, 5 is a No. 3 titration liquid tank, 6 is a No. 1 titration pump, 7 is a No. 2 titration pump, 8 is a No. 3 titration pump, 9 is a peristaltic pump, 10 is a pH electrode, 11 is a pipeline a, 12 is an electromagnetic valve, 13 is an integrated circuit, 14 is a pipeline b, 15 is an indicator lamp, 16 is a titration liquid tank bracket, 17 is a pH electrode bracket, 18 is a pH electrode clamp, 19 is a titration liquid tank bracket connecting rod, 20 is a titration liquid tank pipeline bracket, 21 is a pipeline adapter bracket, 22 is a pipeline adapter, 23 is a threaded sleeve, 24 is a titration pump guide rail, 25 is a titration pump shunting block, 26 is a stepping motor gear rack group, 27 is a side cover supporting plate, 28 is a side cover supporting column, 29 is a base, 30 is an electromagnetic stirring bracket connecting rod, 31 is an integrated circuit interface bracket, 32 is a titration pump electromagnetic valve, 33 is a side cover, 34 is an upper cover, 35 is an electromagnetic stirring device upper cover, 36 is an electromagnet upper gland, 37 is an electromagnet lower gland, 38 is an electromagnet, 39 is a direct current motor, 40 is a direct current motor bracket, 41 is an electromagnetic stirring bracket, and 42 is a box body.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-8, an automatic titrator used in conjunction with a Zeta potential meter comprises a box 42, an upper cover 34, a side cover 33, a sample mixing tank 1, a micro electromagnetic stirring device 2, a No. 1 titration liquid tank 3, a No. 2 titration liquid tank 4, a No. 3 titration liquid tank 5, a No. 1 titration pump 6, a No. 2 titration pump 7, a No. 3 titration pump 8, a peristaltic pump 9, a pH electrode 10, a pipeline a11, an electromagnetic valve 12, an integrated circuit 13, a pipeline b14, a titration liquid tank support 16, a pH electrode support 17, a pH electrode clamp 18, a pipeline adapter 22, a threaded sleeve 23, a titration pump guide rail 24, a titration pump splitter 25, a stepping motor gear rack group 26, a side cover support plate 27, a side cover support 28, a base 29 and a pump electromagnetic valve 32;
the pH electrode bracket 17 is in an inverted L shape and is fixed on a base 29;
one end of the sample mixing pool 1 is screwed on a pH electrode clamp 18, a pH electrode 10 is fixed on a pH electrode bracket 17 through the pH electrode clamp 18, and the pH electrode 10 extends into the sample mixing pool 1;
the other end of the sample mixing pool 1 is suspended on the miniature electromagnetic stirring device 2;
the side cover supporting plate 27 is fixed on the base 29, and the side surface of one end of the side cover supporting plate 27 is provided with a titration liquid pool pipeline bracket 20;
the pipeline adapter 22 is arranged at the top end of the side cover supporting plate 27 through the pipeline adapter support 21, the titration pump diverting block 25 and the peristaltic pump 9 are placed at the upper end of the front side of the side cover supporting plate 27 in parallel, the titration pump guide rail 24 and the gear rack group 26 of the stepping motor are placed on the side cover supporting plate 27 at the lower end of the titration pump diverting block 25 in parallel, and the side cover support columns 28 are fixed on the side cover supporting plate 27 at the two sides of the titration pump guide rail 24;
the titration pump electromagnetic valve 32 is fixed on the titration pump diverting block 25, and the side cover 33 is fixed on the side cover supporting plate 27 through the side cover supporting column 28;
the integrated circuit interface bracket 31 is arranged at the opposite side of the pH electrode bracket 17, the integrated circuit 13 is arranged at the rear side of the side cover supporting plate 27 and is fixed on the base 29 through the integrated circuit interface bracket 31, the signal input end of the integrated circuit 13 receives the instructions of the pH electrode 10 and the control unit, and the output end outputs control signals to be connected to the miniature electromagnetic stirring device 2, the No. 1 titration pump 6, the No. 2 titration pump 7, the No. 3 titration pump 8 and the peristaltic pump 9;
the side cover supporting plate 27, the integrated circuit interface bracket 31 and the pH electrode bracket 17 are fastened and fixed on the base 29 by the box body 42;
the electromagnetic stirring device 2 passes through the pH electrode support 17 through an electromagnetic stirring support connecting rod 30 and is connected with the box body 42;
the titrant pool support 16 is fixed on the box body 42 through a titrant pool support connecting rod 19;
a No. 1 titration liquid pool 3, a No. 2 titration liquid pool 4 and a No. 3 titration liquid pool 5 are arranged on the titration liquid pool support 16;
the No. 1 titration liquid pool 3, the No. 2 titration liquid pool 4 and the No. 3 titration liquid pool 5 penetrate through a titration liquid pool pipeline bracket 20 through a pipeline b to be correspondingly connected with the No. 1 titration pump 6, the No. 2 titration pump 7 and the No. 3 titration pump 8;
the indicator light 15 is fixed on the box body 42;
the upper cover 34 is buckled at the upper ends of the box body 42 and the side cover 33, and the pH electrode 10 of the upper cover 34 penetrates out of the mounting port;
the pipeline a which passes through the pipeline adapter 22 by the peristaltic pump 9 and is connected with the electromagnetic valve 12 passes through the notch of the upper cover 34 and is exposed outside, and the pipeline a is used for sample circulation and is connected with the capillary electrode.
The miniature electromagnetic stirring device 2 comprises an electromagnetic stirring device upper cover 35, an electromagnet upper gland 36, an electromagnet lower gland 37, an electromagnet 38, a direct current motor 39, a direct current motor support 40 and an electromagnetic stirring support 41;
the electromagnet 38 is pressed and fixed on the shaft of the direct current motor 39 through the electromagnet upper gland 36 and the electromagnet lower gland 37;
the direct current motor 39 is fixed on the direct current motor bracket 40, and is fixed on the electromagnetic stirring bracket 41 together with the electromagnetic stirring device upper cover 35.
The titrant is respectively placed in a No. 1 titrant pool 3, a No. 2 titrant pool 4 and a No. 3 titrant pool 5, and the titrant comprises but is not limited to acid, alkali and salt.
The minimum titration volume of the titration solution is 1.68ul without upper limit requirement;
the peristaltic pump 9 has a pump flow rate adjustable in the range of 0-45 ml/min, and has the capability of transferring the titrated sample to the capillary electrode and transferring the Zeta-potential-test-terminated sample back to the mixing cell.
The stirring speed of the miniature electromagnetic stirring device 2 is controllable within the range of 0-2000 r/min, and a sample and the titration solution are uniformly mixed by generating vortex through magnetons in the electric sample mixing pool.
A method of using an automatic titrator in conjunction with a Zeta potential meter, comprising the steps of: the method comprises the following steps: a sample to be detected is placed in a sample mixing pool 1, titration test conditions are set at a software end, a titration solution is respectively placed in a No. 1 titration pool 3, a No. 2 titration pool 4 and a No. 3 titration pool 5, information of the titration solution in the No. 1 titration pool 3, the No. 2 titration pool 4 and the No. 3 titration pool 5, including pH and salinity, is input into software, the volume of the sample in the sample mixing pool is input, and the upper and lower limit ranges of the initial and final solution environments for titration and the titration steps and the number of steps performed in the range are set;
step two: in the first-step titration process, firstly reading the pH value of a current sample through a pH electrode 10, comparing the target pH value set in the first step, carrying out the titration by logically judging and selecting the titration solution in a No. 1 titration cell 3, a No. 2 titration cell 4 and a No. 3 titration cell 5, adding the required amount of the titration solution obtained by calculation into the sample solution in a sample mixing cell 1 through a corresponding titration pump, uniformly mixing the solution and the titration solution through electromagnetic stirring, calculating and confirming the volume of the titration solution needing to be continuously added into the sample through a feedback signal of a pH electrode sensor, closing the electromagnetic stirring if the target pH value is reached, starting an electromagnetic valve, finishing the titration, and carrying out the titration operation in a reciprocating and circulating manner until the target pH value set in the first step is reached if the target pH value is not reached;
step three: the peristaltic pump 9 injects a sample reaching a target pH value into a capillary electrode which is inserted into a Zeta potentiometer host in advance through a pipeline a connected with the capillary electrode, and the sample performs Zeta potential detection in the Zeta potentiometer host through closing a flow path by an electromagnetic valve;
step four: after the detection is finished, a flow path is opened through a solenoid valve, a peristaltic pump 9 conveys the sample from the capillary electrode back to the sample mixing pool 1, and then the second step of titration and test operation is carried out;
step five: and circulating until the sample reaches the set termination solution environment.
The method for selecting the type of the titration solution in the pH titration process comprises the following steps:
1) reading the pH value of the current sample, and comparing the pH value with a target pH value;
2) respectively calculating the sample adding amount required by No. 1-3 titration solutions to reach the target pH value and sequencing;
3) if the lowest sample adding amount is more than 1.68ul, titrating by using the titration solution corresponding to the lowest sample adding amount;
4) if the lowest sample adding amount is less than 1.68ul, comparing whether the second lowest sample adding amount is less than 1.68 mu L, and if so, titrating by using a titration solution corresponding to the second lowest sample adding amount;
5) and 4) if not, titrating the titration solution corresponding to the highest sample adding amount.
The matched software system is compatible with a Zeta potentiometer software system, and before the test is started, the software system can be used for setting:
1) type of titration including, but not limited to, pH, salinity, and concentration of additives;
2) initiating and terminating a step interval within the solution environmental parameter range;
3) redundancy over a range of starting and ending solution environmental parameters;
4) defining each kind of titration liquid, including but not limited to acid, alkali, salt, additive;
5) the system has SOP capability, and is convenient for a user to make, store and call standard operation procedures.
A matched software system can be compatible with a Zeta potentiometer software system, and the software system can calculate according to the test type, the sensor feedback signal and the input parameter in the test process:
1) the type of the titration solution required to be added to achieve the target solution environment;
2) the volume of the titration solution required to reach the target solution environment;
3) and (3) adding the titrating solution to reach the target solution environment.
A matched software system can be compatible with a Zeta potentiometer software system, and the software system can communicate with a control circuit of the automatic titrator and issue commands according to a calculation result obtained by a test type, a sensor feedback signal and an input parameter in the test process.
Claims (10)
1. An automatic titrator used together with a Zeta potentiometer is characterized in that: the device comprises a box body, an upper cover, a side cover, a sample mixing pool, a miniature electromagnetic stirring device, a No. 1 titration liquid pool, a No. 2 titration liquid pool, a No. 3 titration liquid pool, a No. 1 titration pump, a No. 2 titration pump, a No. 3 titration pump, a peristaltic pump, a pH electrode, a pipeline a, an electromagnetic valve, an integrated circuit, a pipeline b, a titration liquid pool support, a pH electrode clamp, a pipeline adapter, a threaded sleeve, a titration pump guide rail, a titration pump shunting block, a stepping motor, a gear rack group, a side cover support plate, a side cover support column, a base and a titration pump electromagnetic valve;
the pH electrode bracket is in an inverted L shape and is fixed on the base;
one end of the sample mixing pool is screwed on a pH electrode clamp through threads, a pH electrode is fixed on a pH electrode bracket through the pH electrode clamp, and the pH electrode extends into the sample mixing pool;
the other end of the sample mixing pool is suspended on the miniature electromagnetic stirring device;
the side cover supporting plate is fixed on the base, and a titration liquid pool pipeline bracket is arranged on the side surface of one end of the side cover supporting plate;
the pipeline adapter is arranged at the top end of the side cover supporting plate through a pipeline adapter support, the titration pump shunt block and the peristaltic pump are arranged at the upper end of the front side of the side cover supporting plate in parallel, the titration pump guide rail and the gear rack group of the stepping motor are arranged on the side cover supporting plate at the lower end of the titration pump shunt block in parallel, and the side cover support columns are fixed on the side cover supporting plate at two sides of the titration pump guide rail;
the titration pump electromagnetic valve is fixed on the titration pump shunting block, and the side cover is fixed on the side cover supporting plate through a side cover strut;
the integrated circuit interface bracket is arranged on the opposite side of the pH electrode bracket, the integrated circuit is arranged on the rear side of the side cover supporting plate and is fixed on the base through the integrated circuit interface bracket, the signal input end of the integrated circuit receives the instructions of the pH electrode and the control unit, and the output end outputs control signals to be connected to the miniature electromagnetic stirring device, the No. 1 titration pump, the No. 2 titration pump, the No. 3 titration pump and the peristaltic pump;
the side cover supporting plate, the integrated circuit interface bracket and the pH electrode bracket are fastened and fixed on the base by the box body;
the electromagnetic stirring device penetrates through the pH electrode support through an electromagnetic stirring support connecting rod and is connected with the box body;
the titrant pool support is fixed on the box body through a titrant pool support connecting rod;
the titrant pool support is provided with a No. 1 titrant pool, a No. 2 titrant pool and a No. 3 titrant pool;
the No. 1 titration liquid pool, the No. 2 titration liquid pool and the No. 3 titration liquid pool penetrate through the titration liquid pool pipeline bracket through the pipeline b and are correspondingly connected with the No. 1 titration pump, the No. 2 titration pump and the No. 3 titration pump;
the indicator light is fixed on the box body;
the upper cover is buckled at the upper ends of the box body and the side cover, and a pH electrode of the upper cover penetrates out of a mounting port arranged on the upper cover;
the pipeline a which passes through the pipeline adapter through the peristaltic pump and is connected with the electromagnetic valve penetrates out of the upper cover notch, and the pipeline a is used for a pipeline which is connected with the capillary electrode and used for sample circulation.
2. An automatic titrator for use with a Zeta potential meter as defined in claim 1, wherein: the miniature electromagnetic stirring device comprises an electromagnetic stirring device upper cover, an electromagnet upper gland, an electromagnet lower gland, an electromagnet, a direct current motor support and an electromagnetic stirring support;
the electromagnet is tightly pressed with the electromagnet lower gland through the electromagnet upper gland and is fixed on a direct current motor shaft;
the direct current motor is fixed on the direct current motor support and is fixed on the electromagnetic stirring support together with the electromagnetic stirring device upper cover.
3. An automatic titrator for use with a Zeta potential meter as defined in claim 1, wherein: the titration solutions are respectively placed in a No. 1 titration solution pool, a No. 2 titration solution pool and a No. 3 titration solution pool, and the titration solutions comprise but are not limited to acids, alkalis and salts.
4. An automatic titrator for use with a Zeta potential meter as defined in claim 3, wherein: the minimum titration volume of the titration solution is 1.68ul without upper limit requirement;
the pump flow of the peristaltic pump is adjustable within the range of 0-45 ml/min, and the peristaltic pump has the capacity of conveying a titrated sample into the capillary electrode and conveying a Zeta-potential-test-finished sample back to the mixing pool.
5. An automatic titrator for use with a Zeta potential meter as defined in claim 2, wherein: the stirring speed of the miniature electromagnetic stirring device is controllable within the range of 0-2000 rpm, and the sample and the titration solution are uniformly mixed by generating vortex through magnetons in the electric sample mixing pool.
6. Use of an automatic titrator in conjunction with a Zeta potential meter as defined in claims 1-5, characterized in that:
the method comprises the following steps: placing a sample to be detected in a sample mixing pool, setting titration test conditions at a software end, respectively placing titration solutions in a No. 1 titration pool, a No. 2 titration pool and a No. 3 titration pool, inputting information of the titration solutions in the No. 1 titration pool, the No. 2 titration pool and the No. 3 titration pool in software, including pH and salinity, inputting the volume of the sample in the sample mixing pool, and setting upper and lower limit ranges of initial and final solution environments for titration and titration steps and step numbers performed in the ranges;
step two: in the first step of titration, firstly reading the pH value of a current sample through a pH electrode, comparing the target pH value set in the first step, selecting a titration solution in a No. 1 titration cell, a No. 2 titration cell and a No. 3 titration cell to perform the titration in the first step through logic judgment, adding a required amount of the titration solution obtained through calculation into a sample solution in a sample mixing cell by a corresponding titration pump, uniformly mixing the solution and the titration solution through electromagnetic stirring, calculating and confirming the volume of the titration solution needing to be continuously added into the sample through a feedback signal of a pH electrode sensor, closing the electromagnetic stirring if the target pH value is reached, starting the electromagnetic valve, finishing the titration, and performing reciprocating circular titration operation until the target pH value set in the first step is reached if the target pH value is not reached;
step three: injecting a sample reaching a target pH value into a capillary electrode which is inserted into a Zeta potentiometer host in advance through a pipeline a connected with the capillary electrode by a peristaltic pump, closing a flow path through an electromagnetic valve, and carrying out Zeta potential detection on the sample in the Zeta potentiometer host;
step four: after the detection is finished, opening a flow path through an electromagnetic valve, conveying the sample from the capillary electrode to the sample mixing pool through a peristaltic pump, and then carrying out second-step titration and test operation;
step five: and circulating until the sample reaches the set termination solution environment.
7. Use of an auto-titrator in conjunction with a Zeta-potentiometer according to claim 6, characterized in that: the method for selecting the type of the titration solution in the pH titration process comprises the following steps:
1) reading the pH value of the current sample, and comparing the pH value with a target pH value;
2) respectively calculating the sample adding amount required by No. 1-3 titration solutions to reach the target pH value and sequencing;
3) if the lowest sample adding amount is more than 1.68ul, titrating by using the titration solution corresponding to the lowest sample adding amount;
4) if the lowest sample adding amount is less than 1.68ul, comparing whether the second lowest sample adding amount is less than 1.68 mu L, and if so, titrating by using a titration solution corresponding to the second lowest sample adding amount;
5) and 4) if not, titrating the titration solution corresponding to the highest sample adding amount.
8. Use of an auto-titrator in conjunction with a Zeta-potentiometer according to claim 6, characterized in that: the matched software system is compatible with a Zeta potentiometer software system, and before the test is started, the software system can be used for setting:
1) type of titration including, but not limited to, pH, salinity, and concentration of additives;
2) initiating and terminating a step interval within the solution environmental parameter range;
3) redundancy over a range of starting and ending solution environmental parameters;
4) defining each kind of titration liquid, including but not limited to acid, alkali, salt, additive;
5) the system has SOP capability, and is convenient for a user to make, store and call standard operation procedures.
9. Use of an auto-titrator in conjunction with a Zeta-potentiometer according to claim 8, wherein: a matched software system can be compatible with a Zeta potentiometer software system, and the software system can calculate according to the test type, the sensor feedback signal and the input parameter in the test process:
1) the type of the titration solution required to be added to achieve the target solution environment;
2) the volume of the titration solution required to reach the target solution environment;
3) and (3) adding the titrating solution to reach the target solution environment.
10. A method of using an autotitrator in conjunction with a Zeta potential meter as defined in claim 9, wherein: a matched software system can be compatible with a Zeta potentiometer software system, and the software system can communicate with a control circuit of the automatic titrator and issue commands according to a calculation result obtained by a test type, a sensor feedback signal and an input parameter in the test process.
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| CN111175348A (en) * | 2019-12-27 | 2020-05-19 | 深圳康泰生物制品股份有限公司 | Detection method of isoelectric point of aluminum hydroxide adjuvant and application of detection method in preparation of vaccine |
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| CN111175348A (en) * | 2019-12-27 | 2020-05-19 | 深圳康泰生物制品股份有限公司 | Detection method of isoelectric point of aluminum hydroxide adjuvant and application of detection method in preparation of vaccine |
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