CN114235540B - Automatic smear spray-dyeing device and spray-dyeing method - Google Patents

Automatic smear spray-dyeing device and spray-dyeing method Download PDF

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Publication number
CN114235540B
CN114235540B CN202111384825.4A CN202111384825A CN114235540B CN 114235540 B CN114235540 B CN 114235540B CN 202111384825 A CN202111384825 A CN 202111384825A CN 114235540 B CN114235540 B CN 114235540B
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spray head
cavity
spray
inlet
dyeing
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CN114235540A (en
Inventor
梅茜
董文飞
李力
常智敏
葛明锋
王国伟
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Suzhou Institute of Biomedical Engineering and Technology of CAS
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Suzhou Institute of Biomedical Engineering and Technology of CAS
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B05SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05BSPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B1/00Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means
    • B05B1/02Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops, or having an outlet of particular shape
    • B05B1/06Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops, or having an outlet of particular shape in annular, tubular or hollow conical form
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B05SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05BSPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B1/00Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means
    • B05B1/34Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to influence the nature of flow of the liquid or other fluent material, e.g. to produce swirl
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • G01N2001/317Apparatus therefor spraying liquids onto surfaces

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Fiber Materials (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)

Abstract

The invention discloses an automatic smear spray-dyeing device, which comprises: a spray head core having a spray head inlet and at least one cavity injection port in communication with the spray head inlet; the spray head cap is provided with a spray head cavity, at least two cyclone inlets, a cyclone chamber and a spray head outlet which are communicated in sequence; the spray head core is inserted in the spray head cap in a matched mode, dye liquor enters the spray head cavity through the cavity injection opening after being injected through the spray head inlet, then enters the swirl chamber through the swirl inlet along a tangential direction, swirl is formed in the swirl chamber, and finally the dye liquor is sprayed out of the spray head outlet. The automatic smear spraying and dyeing device provided by the invention has the advantages of uniform dyeing, high dyeing efficiency and the like compared with manual drop dyeing by arranging the small low-pressure cyclone atomization spray head with a plurality of tangential cyclone inlets, and meanwhile, the device provided by the invention has the advantages of simple structure, convenience in maintenance, lower working pressure and convenience in realizing the dyeing automation of smears.

Description

Automatic smear spray-dyeing device and spray-dyeing method
Technical Field
The invention relates to the technical field of clinical examination, in particular to an automatic smear spray-dyeing device and a spray-dyeing method.
Background
Morphological examination of microorganisms in medical tests is an important means for judging clinical pathogens, and many samples such as blood, urine, sputum and the like need to be smeared before microscopic examination, and currently, a manual drip staining mode is usually adopted. For example, microscopic examination of blood smears is a gold standard for morphological analysis of blood cells, and plays an important role in clinical examination, and the quality of the smears also affects pathological diagnosis results. Clinical small-batch blood smear dyeing is usually performed by manually dripping dye liquor by a checking doctor, and the dyeing method has the defects of high dye liquor consumption, uneven dyeing depth and dye deposition caused by uneven mixing of the dye liquor, and finally manufactured blood smears are difficult to realize standardization. Therefore, it is important to improve the staining pattern of the smear.
Disclosure of Invention
The invention aims to solve the technical problem of providing an automatic smear spray-dyeing device aiming at the defects in the prior art.
In order to solve the technical problems, the invention adopts the following technical scheme: an automatic smear spray dyeing apparatus comprising:
a spray head core having a spray head inlet and at least one cavity injection port in communication with the spray head inlet;
the spray head cap is provided with a spray head cavity, at least two cyclone inlets, a cyclone chamber and a spray head outlet which are communicated in sequence;
the cyclone chamber comprises a cylindrical cavity section and an inverted cone-shaped cavity section which are communicated with each other, and an output port of the cyclone inlet is tangentially communicated with the cylindrical cavity section;
the spray head core is inserted in the spray head cap in a matched mode, dye liquor enters the spray head cavity through the cavity injection opening after being injected through the spray head inlet, then enters the swirl chamber through the swirl inlet along a tangential direction, swirl is formed in the swirl chamber, and finally the dye liquor is sprayed out of the spray head outlet.
Preferably, the cavity injection port communicates with the spray head inlet through a delivery channel.
Preferably, the nozzle core comprises a core body and an injection head connected to the tail end of the core body, the conveying channel is formed in the core body, and the nozzle inlet is formed at the front end of the core body;
the side wall of the injection head is provided with at least one slot communicated with the conveying channel, and the slot and the communication port of the conveying channel form the cavity injection port.
Preferably, a column hole is formed in the spray head cap from the bottom of the spray head cavity downwards, and the column cavity section is communicated with the bottom of the column hole.
Preferably, at least 2 rectangular grooves which penetrate through the column holes and are communicated with the cylindrical cavity section are further formed in the bottom of the nozzle cavity downwards, and the ports, communicated with the cylindrical cavity section, of the rectangular grooves form the rotational flow inlet.
Preferably, an opening communicated with the nozzle outlet is formed in the bottom of the nozzle cap, and the diameter of the opening is larger than that of the nozzle outlet.
Preferably, the periphery of the core body is also provided with an annular upper positioning flange, and the upper end of the nozzle cap is provided with an annular lower positioning flange.
Preferably, after the nozzle core is inserted into the nozzle cap, the core body is inserted into the nozzle cavity in a matched manner, the upper positioning flange is pressed against the lower positioning flange in a matched manner, and the injection head is inserted into the column hole in a matched manner; at this time, the conveying channel is communicated with the spray head cavity through the slot, and the spray head cavity is communicated with the spray head outlet through the rectangular slot, the cylindrical cavity section and the inverted cone cavity section in sequence.
Preferably, the rectangular slot comprises 4, forming 4 swirl inlets arranged at uniform intervals, and the slot comprises 2, forming 2 cavity injection ports.
Preferably, the diameter of the nozzle inlet is 3mm, the length and the width of the rectangular groove are respectively 0.5mm and 0.3mm, the inner diameter of the upper port of the inverted cone-shaped cavity section is 2mm, and the inner diameter of the lower port is 0.4mm;
the pressure of the dye liquor injected into the inlet of the spray head is more than 0 and not more than 0.3MPa.
Preferably, the step of staining the smear with the device comprises:
1) Pre-measuring the injection pressure of the dye liquor to be P 0 The spray angle of the dye liquor sprayed from the outlet of the spray head is the cone angle of a cone formed by diffusion of the dye liquor after being sprayed from the outlet of the spray head;
2) Determination of the minimum circle O that completely covers the area to be stained on the smear min According to the diameter L ofThe following formula calculates the center of the nozzle outlet to the minimum circle O min Perpendicular distance h between the centers of circles:
3) To make the outlet of the spray head at the minimum circle O min H above the center of the circle of (C) and the pressure is P 0 And (5) injecting a dye solution into the inlet of the spray head, and dyeing the smear.
The beneficial effects of the invention are as follows: the automatic smear spraying and dyeing device provided by the invention has the advantages of uniform dyeing, high dyeing efficiency and the like compared with manual drop dyeing by arranging the small low-pressure cyclone atomization spray head with a plurality of tangential cyclone inlets, and meanwhile, the device provided by the invention has the advantages of simple structure, convenience in maintenance, lower working pressure and convenience in realizing the dyeing automation of smears.
Drawings
FIG. 1 is a schematic diagram of the automatic smear spray dyeing apparatus of the present invention;
FIG. 2 is a schematic diagram of a spray head core according to the present invention;
FIG. 3 is a schematic view of the structure of the shower cap of the present invention;
FIG. 4 is a schematic view showing the internal structure of the automatic smear spraying and dyeing apparatus of the present invention;
FIG. 5 is a schematic cross-sectional view of the automatic smear spray dyeing apparatus of the present invention;
FIG. 6 is a cross-sectional view of the spray head cap of the present invention in a cross-sectional direction;
FIG. 7 is a schematic illustration of a dye liquor coverage area in an embodiment of the invention;
FIG. 8 is a schematic view of the atomization angle at different injection pressures in an embodiment of the present invention;
fig. 9 is a microscopic image of a stained blood smear in an embodiment of the invention.
Reference numerals illustrate:
1-a spray head core; 10-a core; 11-an injection head; 12-slotting; 13-upper positioning flange;
2-a spray head cap; 20-column holes; 21-rectangular grooves; 22-a lower locating flange; 23-perforating;
3-a nozzle inlet; 4-a conveying channel; 5-a cavity injection port; 6-a nozzle cavity; 7-a swirl inlet;
8-a swirl chamber; 80-a cylindrical cavity section; 81-an inverted cone cavity section;
9-nozzle outlet.
Detailed Description
The present invention is described in further detail below with reference to examples to enable those skilled in the art to practice the same by referring to the description.
It will be understood that terms, such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
As shown in fig. 1 to 6, an automatic smear spray-dyeing apparatus of the present embodiment includes:
a head core 1 having a head inlet 3 and at least one cavity injection port 5 communicating with the head inlet 3;
and a head cap 2 having a head cavity 6, at least two swirl inlets 7, a swirl chamber 8 and a head outlet 9 which are sequentially communicated;
wherein the swirl chamber 8 comprises a cylindrical cavity section 80 and an inverted cone-shaped cavity section 81 which are communicated with each other, and an output port of the swirl inlet 7 is tangentially communicated with the cylindrical cavity section 80;
the spray head core 1 is matched and inserted in the spray head cap 2, dye liquor enters the spray head cavity 6 through the cavity injection opening 5 after being injected by the spray head inlet 3, then enters the swirl chamber 8 through the swirl inlet 7 along the tangential direction, forms swirl in the swirl chamber 8, and finally is sprayed out from the spray head outlet 9.
In this embodiment, the cavity injection port 5 communicates with the shower head inlet 3 through the delivery channel 4. The nozzle core 1 comprises a core body 10 and an injection head 11 connected to the tail end of the core body 10, wherein a conveying channel 4 is formed in the core body 10, and a nozzle inlet 3 is formed at the front end of the core body 10; the side wall of the injection head 11 is provided with at least one slot 12 communicated with the conveying channel 4, and the communication port between the slot 12 and the conveying channel 4 forms a cavity injection port 5. In the preferred embodiment, the slot 12 comprises 2, with 2 cavity injection ports 5 formed.
In this embodiment, the bottom of the nozzle cavity 6 in the nozzle cap 2 is provided with a column hole 20 downwards, and the column cavity section 80 is communicated with the bottom of the column hole 20. The bottom of the nozzle cavity 6 is also provided with at least 2 rectangular grooves 21 which pass through the column holes 20 and are communicated with the cylindrical cavity section 80, and the ports of the rectangular grooves 21 communicated with the cylindrical cavity section 80 form a rotational flow inlet 7. In the preferred embodiment, the rectangular slot 21 comprises 4, forming 4 swirl inlets 7 arranged at even intervals.
In this embodiment, the bottom of the nozzle cap 2 is provided with an opening 23 communicating with the nozzle outlet 9, and the diameter of the opening 23 is larger than the diameter of the nozzle outlet 9.
In this embodiment, the outer periphery of the core 10 is further provided with an annular upper positioning flange 13, and the upper end of the head cap 2 is provided with an annular lower positioning flange 22. After the spray head core 1 is inserted into the spray head cap 2, the core body 10 is matched and inserted into the spray head cavity 6, the upper positioning flange 13 is matched and pressed on the lower positioning flange 22, and the injection head 11 is matched and inserted into the column hole 20; at this time, the delivery channel 4 communicates with the nozzle cavity 6 through the slot 12, and the nozzle cavity 6 communicates with the nozzle outlet 9 through the rectangular slot 21, the cylindrical cavity section 80 and the inverted cone cavity section 81 in this order.
In a preferred embodiment, the diameter of the nozzle inlet 3 is 3mm, the length and width of the rectangular slot 21 are 0.5mm and 0.3mm respectively, the inner diameter of the upper port of the inverted conical cavity section 81 is 2mm, and the inner diameter of the lower port is 0.4mm; the pressure of the dye liquor injection nozzle inlet 3 is 0.2-0.3MPa.
The working process comprises the following steps:
the dye liquor is injected from the nozzle inlet 3 under the pressure effect, then enters the nozzle cavity 6 through the 2 cavity injection openings 5, then enters the cyclone chamber 8 along the tangential direction through the 4 cyclone inlets 7 to form cyclone, and moves at high speed along the inner wall of the conical cyclone chamber 8 under the centrifugal force effect, because the reverse conical structure is adopted below the cyclone chamber 8, when the dye liquor flows to the tail end of the cyclone chamber 8, the dye liquor can obtain larger axial speed and radial speed, so the dye liquor can be sprayed out in the form of a conical liquid film at the nozzle outlet 9, and the high-speed liquid film moves relatively with air, tears and breaks into fine fog drops.
The spray angle of the dye solution sprayed from the spray outlet 9 is related to the space region of the spray field and the distribution of the fog drops, and the dye solution is diffused into an approximately conical spray form after being sprayed from the spray outlet 9, and the height of the spray outlet 9 from the smear can be calculated according to the size of the region to be dyed of the smear by measuring the spray angle, so that spray dyeing according to the required region size can be ensured. Wherein, the atomizing angle is the cone angle of a cone formed by diffusion after the dye liquor is sprayed out from the nozzle outlet 9, and the cone angle is as follows: the angle between two generatrices of the axial section of the cone (the section of the axis passing through the cone); the atomizing field is the bottom surface of the cone, i.e., the spray-dyed area.
In a preferred embodiment, the step of staining the smear with the device comprises:
1) Pre-measuring the injection pressure of the dye liquor to be P 0 The atomizing angle of the dye liquor sprayed from the nozzle outlet 9 is the cone angle of a cone formed by diffusion of the dye liquor after sprayed from the nozzle outlet 9;
2) Referring to FIG. 7, a minimum circle O is determined that completely covers the area to be stained on the smear min Is calculated from the center of the shower nozzle outlet 9 to the minimum circle O according to the following formula min Perpendicular distance h between the centers of circles:
3) To make the nozzle outlet 9 at the minimum circle O min H above the center of the circle of (C) and the pressure is P 0 And (3) injecting a dye solution into the nozzle inlet 3 to dye the smear.
For example, in one embodiment, images of the atomization angle under the conditions of the dye liquor injection pressure of 0.1, 0.12, 0.14, 0.16, 0.18, 0.2, 0.22 and 0.24MPa are acquired, corresponding atomization angles are measured, in order to reduce measurement errors, the size of the atomization angles is accurately estimated, five images are respectively taken for measurement at each test pressure, and an average value is taken; angular measurements were made by ImageJ software and with reference to fig. 8, it can be seen that the spray angle of the spray head increased from 56.72 ° to 77.17 ° as the injection pressure increased gradually from 0.1MPa to 0.24 MPa. When the injection pressure reaches 0.2MPa, the atomization angle is finally stabilized between 76 DEG and 78 deg. From the above data, it was found that the minimum distance parameter between the smear and the showerhead was between 34 and 51mm within the selected pressure range of 0.1-0.24 MPa.
In a further embodiment, the device is applied to blood smear staining and compared to artificial drip staining effects. Specifically, two smears were subjected to Rui-Jim Sa spray staining (using the apparatus of the present invention) and conventional manual drip staining, respectively. The parameters of spray-dyeing are that a smear is placed under a spray nozzle for 35mm, the injection pressure is 0.2MPa, and blood cells after dyeing, washing and drying are observed under a microscope as shown in fig. 9 (a), so that the nuclei are purplish red, the nuclear chromatin structure is clear, and a better dyeing effect is achieved. Compared with the manual drop dyeing of fig. 9 (b), the spray dyeing of fig. 9 (a) has higher spray dyeing efficiency, more uniform dyeing and lighter background color, and is beneficial to subsequent operations of identification, classification, counting and the like.
It will be appreciated that the device of the present invention is also applicable to smear staining of microorganisms and other pathogens.
Although embodiments of the present invention have been disclosed above, it is not limited to the use of the description and embodiments, it is well suited to various fields of use for the invention, and further modifications may be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the particular details without departing from the general concepts defined in the claims and the equivalents thereof.

Claims (4)

1. An automatic smear spray-dyeing device, comprising:
a spray head core having a spray head inlet and at least one spray head cavity injection port in communication with the spray head inlet;
the spray head cap is provided with a spray head cavity, at least two cyclone inlets, a cyclone chamber and a spray head outlet which are communicated in sequence;
the cyclone chamber comprises a cylindrical cavity section and an inverted cone-shaped cavity section which are communicated with each other, and an output port of the cyclone inlet is tangentially communicated with the cylindrical cavity section;
the spray head core is inserted in the spray head cap in a matching way, dye liquor enters the spray head cavity through the spray head cavity injection opening after being injected from the spray head inlet, then enters the swirl chamber through the swirl inlet along a tangential direction, forms swirl in the swirl chamber, and finally is sprayed out from the spray head outlet;
the nozzle cavity injection port is communicated with the nozzle inlet through a conveying channel;
the spray head core comprises a core body and an injection head connected to the tail end of the core body, the conveying channel is formed in the core body, and the spray head inlet is formed in the front end of the core body;
the side wall of the injection head is provided with at least one slot communicated with the conveying channel, and the slot and a communication port of the conveying channel form the injection port of the nozzle cavity;
a column hole is formed in the spray head cap downwards from the bottom of the spray head cavity, and the column cavity section is communicated with the bottom of the column hole;
at least 2 rectangular grooves which penetrate through the column holes and are communicated with the cylindrical cavity section are formed in the bottom of the spray head cavity downwards, and the rectangular grooves and ports communicated with the cylindrical cavity section form the rotational flow inlet;
the periphery of the core body is also provided with an annular upper positioning flange, and the upper end of the nozzle cap is provided with an annular lower positioning flange;
after the spray head core is inserted into the spray head cap, the core body is inserted into the spray head cavity in a matched mode, the upper positioning flange is pressed on the lower positioning flange in a matched mode, and the injection head is inserted into the column hole in a matched mode; at this time, the conveying channel is communicated with the spray head cavity through the slot, and the spray head cavity is communicated with the spray head outlet through the rectangular slot, the cylindrical cavity section and the inverted cone cavity section in sequence.
2. The automatic smear spray-dyeing apparatus according to claim 1, wherein the rectangular grooves comprise 4, forming 4 swirl inlets uniformly spaced, and the grooves comprise 2, forming 2 nozzle cavity inlets.
3. The automatic smear spraying and dyeing device according to claim 2, wherein the diameter of the inlet of the spray nozzle is 3mm, the length and width of the rectangular groove are 0.5mm and 0.3mm respectively, the inner diameter of the upper port of the inverted cone cavity section is 2mm, and the inner diameter of the lower port is 0.4mm;
the pressure of the dye liquor injected into the inlet of the spray head is more than 0 and not more than 0.3MPa.
4. A smear automatic spray dyeing apparatus according to any one of claims 1 to 3, characterized in that the step of dyeing the smear by the apparatus comprises:
1) Pre-measuring the injection pressure of the dye liquor to be P 0 The spray angle of the dye liquor sprayed from the outlet of the spray head is the cone angle of a cone formed by diffusion of the dye liquor after being sprayed from the outlet of the spray head;
2) Determination of the minimum circle O that completely covers the area to be stained on the smear min Is calculated from the center of the nozzle outlet to the minimum circle O according to the following formula min Perpendicular distance h between the centers of circles:
3) To make the outlet of the spray head at the minimum circle O min H above the center of the circle of (C) and the pressure is P 0 And (5) injecting a dye solution into the inlet of the spray head, and dyeing the smear.
CN202111384825.4A 2021-11-22 2021-11-22 Automatic smear spray-dyeing device and spray-dyeing method Active CN114235540B (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104368462A (en) * 2014-11-21 2015-02-25 浙江爱力浦科技股份有限公司 Spiral flow type methanol injection atomizer
CN105855078A (en) * 2016-06-15 2016-08-17 北京航空航天大学 Centrifugal nozzle with multihole cyclone wall and spray method
CN207076573U (en) * 2016-11-30 2018-03-09 福建天广消防有限公司 A kind of pressure type foam spray nozzle
CN110449283A (en) * 2019-09-12 2019-11-15 河北工业大学 A kind of novel atomized nozzle based on bubble cutting
CN212090635U (en) * 2020-02-28 2020-12-08 萃联(中国)消防设备制造有限公司 Vortex spraying atomizing nozzle
CN112254125A (en) * 2020-10-14 2021-01-22 华中科技大学 Spiral-flow type pressure atomizing nozzle

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120168538A1 (en) * 2009-10-16 2012-07-05 Tiefu Han Spin Annular Slit Spray Nozzle and Spray Apparatus Thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104368462A (en) * 2014-11-21 2015-02-25 浙江爱力浦科技股份有限公司 Spiral flow type methanol injection atomizer
CN105855078A (en) * 2016-06-15 2016-08-17 北京航空航天大学 Centrifugal nozzle with multihole cyclone wall and spray method
CN207076573U (en) * 2016-11-30 2018-03-09 福建天广消防有限公司 A kind of pressure type foam spray nozzle
CN110449283A (en) * 2019-09-12 2019-11-15 河北工业大学 A kind of novel atomized nozzle based on bubble cutting
CN212090635U (en) * 2020-02-28 2020-12-08 萃联(中国)消防设备制造有限公司 Vortex spraying atomizing nozzle
CN112254125A (en) * 2020-10-14 2021-01-22 华中科技大学 Spiral-flow type pressure atomizing nozzle

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