CN114231740B - Method for decomposing monazite by utilizing composite microorganisms - Google Patents
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- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B3/00—Extraction of metal compounds from ores or concentrates by wet processes
- C22B3/18—Extraction of metal compounds from ores or concentrates by wet processes with the aid of microorganisms or enzymes, e.g. bacteria or algae
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B59/00—Obtaining rare earth metals
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- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B60/00—Obtaining metals of atomic number 87 or higher, i.e. radioactive metals
- C22B60/02—Obtaining thorium, uranium, or other actinides
- C22B60/0291—Obtaining thorium, uranium, or other actinides obtaining thorium
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Abstract
The invention discloses a method for decomposing monazite by utilizing composite microorganisms, belonging to the technical field of microorganism hydrometallurgy. In the invention, monazite concentrate is leached and decomposed in two steps through compound microorganisms. The first step is to add a certain amount of composite phosphate-dissolving microbial bacteria liquid into a reaction kettle, add a certain amount of monazite concentrate powder, uniformly mix, start leaching under a set condition, reduce the pH value of the bacteria liquid in the reaction kettle after a period of time, and perform the second step of leaching. Finally, the solution is separated and extracted, microorganisms can be separated and recycled, and the rest soluble phosphorus can be used for producing phosphate fertilizer for promoting the growth of crops. The method uses the wastes in industrial and agricultural production as energy sources, the generated organic acid can be decomposed by microorganisms in the nature, the influence on the environment is small, the generated organic acid is weak acid, the loss of the apparatus is reduced, the environment is not polluted, the production cost is low, the environment-friendly metallurgical requirement of the current society is met, and the method has important practical significance.
Description
Technical Field
The invention belongs to the technical field of microorganism hydrometallurgy, and particularly relates to a method for decomposing monazite by utilizing composite microorganisms.
Background
Monazite is phosphate mineral of rare earth and thorium, and has chemical molecular expression of (Ce, la, th) PO 4 . The mineral content of monazite in concentrate can reach 95% -98%, wherein P 2 O 5 The content is 25-27%, and REO is 50-60%. Monazite other than that containingRare earth and phosphorus, and also contains more radioactive elements Th, U and trace amounts of radioactive decay products Ra of Th and U, and also contains a small amount of rutile (TiO 2 ) Ilmenite (FeO, tiO) 2 ) Zircon (ZrO) 2 ·SiO 2 ) Quartz (SiO) 2 ) And the like.
In recent years, with the frequent occurrence of various problems in the development and utilization of global resources, particularly rare earth resources, and in the international trade process, the development and utilization of monazite ores are increasingly receiving importance from the countries and society. Although there are various methods of decomposing monazite in the research work field, there are only two processes of concentrated sulfuric acid decomposition and caustic soda decomposition in industrial production. The concentrated sulfuric acid roasting method has the greatest advantages of strong adaptability to concentrate, and satisfactory results can be obtained even if the content of valuable elements in the concentrate is low and the particles are coarse. The defects are that: the acid gas is easy to corrode, so that great difficulty is brought to labor protection and environmental protection, and the phosphorus with the content lower than rare earth in the concentrate is difficult to recycle. The advantages and disadvantages of the caustic soda process are exactly opposite to those of the concentrated sulfuric acid process. The equipment corrosion, labor protection and environmental protection in the caustic soda process are easy to solve, and the phosphorus in the monazite can be recovered. However, it requires monazite concentrate with as little impurity content as possible, and the concentrate is ground and pre-decalcified before decomposition, so that the method has the defects of high energy consumption, low equipment utilization rate, high caustic soda price and the like.
Aiming at the decomposition of monazite, ma Shengfeng of Baotou rare earth institute adopts magnesium chloride to decompose monazite except an acid method and an alkali method, and the result shows that: magnesium oxide and hydrogen chloride, which are the decomposition products of basic magnesium chloride, react with monazite at 500 ℃ to produce REOCl, which is further decomposed at 550 ℃ to produce rare earth composite oxide. The roasting temperature, the roasting time and the mineral-salt ratio have no obvious influence on the decomposition rate of the monazite. The hydrogen chloride and the water have catalytic decomposition effect in the decomposition process of the monazite, and the decomposition rate of the monazite can be improved by controlling the roasting temperature in stages by utilizing the catalytic effect. The biological metallurgical technology has early application in copper, gold, zinc and lead smelting industry, but has less application in phosphorus-containing minerals, and patent CN201711241331.4 discloses a method for dephosphorizing high-phosphorus iron ore by using Aspergillus niger strain fermentation liquor.
Disclosure of Invention
The invention aims to provide a method for decomposing monazite ore by utilizing composite microorganisms, which decomposes monazite by two-step microorganism leaching, has the advantages of small equipment loss, no environmental pollution and low production cost, can realize comprehensive recycling and utilization of resources, meets the requirements of green metallurgy in the current society, and has important practical significance.
The aim of the invention is achieved by the following technical scheme:
a method for decomposing monazite by using composite microorganisms, comprising the following steps:
(1) Leaching in the first step: adding the composite phosphate-dissolving microbial bacteria solution, monazite concentrate powder and gibberellin into a reaction kettle filled with the bacteria solution nutrient solution, and adjusting the initial pH value of the solution to 5.5-7.0 for primary leaching. The compound phosphate-solubilizing microorganism comprises Aspergillus niger, penicillium oxalicum, lactobacillus and bacillus megatherium. In the step, the addition amount of the monazite concentrate powder is preferably 2:1-5:1 of liquid-solid ratio; the final concentration of gibberellin is preferably 1.0-4.0 mg/L; the condition of the first leaching step is that air is introduced at the temperature of 30-37 ℃ and the air is slowly stirred, and the leaching is carried out for 65-75 hours.
(2) Leaching in the second step: adding alpha-naphthylacetic acid into the reaction kettle to reduce the pH value of the solution to 2.5-3.5, and carrying out secondary leaching. In this step, the time for the second leaching is preferably 45 to 50 hours.
In the method, the purpose of the complex phosphate-dissolving microorganism ore leaching is that the phosphate-dissolving microorganism secretes acid metabolites (organic acids) so as to dissolve orthophosphate from insoluble minerals. The microorganisms release metabolites, such as organic acids, which dissolve the metal elements from the minerals by replacing or forming complexes and chelates with the metal ions at a leaching rate 20-100 times faster than chemical leaching. Wherein the organic acid secreted by microorganism and capable of chelating metal ion mainly comprises citric acid, gluconic acid, tartaric acid, oxalic acid, malic acid, lactic acid, succinic acid, salicylic acid, acetic acid, amino acid, etc. The microbial leaching process is mainly the complexing process of organic acid and metal element, and most of organic acid belongs to weak acid, so that the environment is protected, the service life of equipment is prolonged, and the ore does not need to be acidified in advance. The decomposition rate of minerals in the experiment is far lower than that in the case of leaching by microorganism when the organic acid is used for leaching. And the effect of leaching ore by singly utilizing one microorganism is not as good as that of leaching ore by matching a plurality of microorganisms, because the proportion and the amount of organic acid produced by one microorganism are limited, the composite microorganism leaching ore can well solve the problem. Aspergillus niger is used for metabolizing citric acid in a large amount, and gluconic acid, oxalic acid and the like in a small amount (the pH has a great influence on the metabolism of organic acid types by Aspergillus niger); the metabolites of the penicillium oxalicum are mainly oxalic acid, malic acid, citric acid and the like; bacillus megaterium metabolizes gluconic acid at most and certain amino acids; lactic acid bacteria metabolize mainly lactic acid and aerobic respiration produces a certain amount of acetic acid. The cooperation of various microorganisms is obviously more favorable for decomposing monazite and chelating rare earth elements in the monazite.
The nutrient components of the bacterial liquid nutrient solution require: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 and water are uniformly stirred according to the mass ratio of 1:8-15, the sucrose is 55-60 g/L, the glucose is 20-25 g/L, naCl is 0.6-0.9 g/L, KCl 0.8.8-1.2 g/L, mgSO 4 ·7H 2 O 1.2～1.5g/L、NH 4 SO 4 1.7～2.0g/L、FeSO 4 ·7H 2 The nutrient substances in the bacterial liquid nutrient solution composed of the components are more beneficial to the metabolism acid discharge of compound microorganisms, and the decomposition efficiency of phosphorus-containing minerals can be improved by more than 10 percent.
The concentration of the compound phosphate-dissolving microorganisms is 10 6 ～10 8 Inoculating each/mL, wherein each bacterial liquid inoculum size is respectively 5-9% of Aspergillus niger inoculum size, 2-7% of Penicillium oxalicum inoculum size, 1-5% of lactobacillus inoculum size and 2-6% of Bacillus megaterium inoculum size, and the ratio of the inoculum sizes is that the microorganism growth and metabolism are good, the concentration of metabolic organic acid is proper, the ratio is proper, and the leaching effect is improvedThe height is higher than 15 percent.
The granularity of the monazite concentrate powder is-300 to-400 meshes. Bacterial leaching is a process that proceeds at the interface of the ore, the rate of which is closely related to the specific surface area of the solids. The leaching rate of bacteria is influenced by different ore particle sizes, the specific surface area of the bacteria is increased along with the reduction of the ore particle size, the acting area of the bacteria on the ore is increased, and the higher the decomposition rate of the phosphorus-containing minerals is, the higher the speed is.
In the method, the purpose of the initial pH value of the solution is 5.5-7.0, so that the bacillus megatherium accumulates gluconic acid in the pH value range, the aspergillus niger not only can metabolize citric acid under a higher pH value environment, but also can increase the accumulation of gluconic acid, the pH value of 5.5-7.0 is also suitable for the fermentation of lactobacillus and penicillium oxalicum, and four microorganisms can grow and reproduce in large quantities under the pH value, so that preparation is provided for the next second leaching step.
In the method, alpha-naphthylacetic acid is added to reduce the pH value of the solution to 2.5-3.5, so that the Aspergillus niger metabolite is a large amount of citric acid under the acidity condition, the oxalic acid and malic acid which are the products of penicillium oxalicum have extremely strong mineral decomposition capability, and the low pH value is more favorable for the decomposition of monazite, but the too low pH value can inhibit the growth of strains and even deactivate the strains, and the monazite decomposition rate with the pH value of 2.5-3.5 is improved by 6 percent through experiments. Meanwhile, the addition of the alpha-naphthylacetic acid can promote the aspergillus niger to secrete citric acid and oxalic acid.
Further, the method for decomposing the monazite by utilizing the composite microorganism comprises the following steps:
(1) Leaching in the first step: adding the composite phosphate-dissolving microbial bacteria solution into a reaction kettle filled with bacteria solution nutrient solution according to the following inoculation amount: 5 to 9 percent of Aspergillus niger bacterial liquid, 2 to 7 percent of Penicillium oxalicum bacterial liquid, 1 to 5 percent of lactic acid bacterial liquid, 2 to 6 percent of bacillus megaterium bacterial liquid, and 10 percent of each bacterial liquid 6 ～10 8 Inoculating at a concentration of individual/mL; adding monazite concentrate powder of-300 to-400 meshes according to a liquid-solid ratio of 2:1-5:1, adding gibberellin to enable the concentration of gibberellin in the solution to reach 1.0-4.0 mg/L, adjusting the initial pH value of the solution to be 5.5-7.0, introducing air at 30-37 ℃, slowly stirring, and leaching for 65-75 hours.
(2) Leaching in the second step: adding alpha-naphthylacetic acid into the reaction kettle to reduce the pH value of the solution to 2.5-3.5, and leaching for 45-50 h.
In the method, the purpose of slow stirring is that firstly, the thalli are contacted with nutrient substances to sufficiently accelerate the thalli growth; secondly, the metabolite is better transferred and plays a role in the system to accelerate the reaction speed; thirdly, the system is uniform, and bottom precipitation is prevented; the air is introduced to increase dissolved oxygen, three other microorganisms except the lactobacillus are aerobic bacteria, enough oxygen is needed to provide survival and metabolism of the bacteria, and although the oxygen can inhibit the lactobacillus from metabolizing the lactic acid, experiments show that the lactobacillus still can metabolize the lactic acid and the acetic acid in an aerobic environment, and only the lactic acid can be converted into the acetic acid with the time being prolonged, but the leaching effect on the microorganisms is small. Slow agitation is because if the agitation is too rapid, it can affect the propagation of microorganisms.
In the method, gibberellin is added to enable the gibberellin concentration of the solution to reach 1.0-4.0 mg/L, so that the gibberellin can promote Aspergillus niger to secrete gluconic acid and oxalic acid, and the effect is good when the gibberellin concentration is measured to be 1.0-4.0 mg/L through experiments.
The monazite is leached twice by the method, centrifugally filtered, rare earth in the solution is separated and extracted by an ammonium bicarbonate precipitation method, and the rest soluble phosphorus can be used for producing phosphate fertilizer for promoting the growth of crops, and the leaching composite microorganism is recovered and used for next leaching.
The invention has the advantages that: the existing monazite decomposition method is accompanied with the problems of pollution, complex process, high energy consumption and the like, heterotrophic microorganisms can utilize wastes in industrial and agricultural production as energy sources, and the generated organic acid can be decomposed by microorganisms in the nature, so that the method has small influence on the environment, is more suitable for the current situations of current resource shortage and continuous increase of environmental protection pressure of enterprises, and receives the current clean green metallurgical surge. And the cost of decomposing the monazite by microorganisms is low, and the generated acid is weak acid and has little damage to equipment. During separation and utilization, not only can the required rare earth elements be obtained, but also a large amount of phosphorus elements in the monazite can be separated to prepare phosphate fertilizer for plant absorption and utilization, and mineral leaching microorganisms can be recycled. The invention can lead the decomposition rate of monazite minerals to reach more than 97 percent.
Detailed Description
The following examples serve to further illustrate the invention but are not to be construed as limiting the invention. The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated.
Example 1:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 is uniformly stirred with water according to the mass ratio of 1:10, and the mixture comprises 56g/L of sucrose, 22g/L, naCl of glucose 0.6g/L, KCl 1.1.1 g/L, mgSO 4 ·7H 2 O 1.3g/L、NH 4 SO 4 1.9g/L、FeSO 4 ·7H 2 O 0.8g/L。
(2) Adding a composite phosphate-dissolving microbial liquid into a reaction kettle filled with a bacterial liquid nutrient solution, wherein the inoculation amount of each bacterial liquid is as follows: 6% of Aspergillus niger bacterial liquid, 4% of penicillium oxalicum liquid, 2% of lactobacillus (streptococcus thermophilus) bacterial liquid, and 5% of bacillus megaterium bacterial liquid. Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-300 to-400 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 5:1, regulating the initial pH value of the solution to 6.0, slowly stirring through air at 30 ℃, adding gibberellin to ensure that the concentration reaches 1.0mg/L, and leaching for 72 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 2.5, and continuously leaching the ore for 48 hours.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 97.4 percent.
Comparative example 1:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 is uniformly stirred with water according to the mass ratio of 1:10, and the mixture comprises 66g/L of sucrose, 30g/L, naCl of glucose, 1.2g/L, KCl, 0.5g/L, mgSO 4 ·7H 2 O 0.8g/L、NH 4 SO 4 1.5g/L、FeSO 4 ·7H 2 O 1.0g/L。
(2) Is filled with bacterial liquidAdding composite phosphate-dissolving microbial bacteria liquid into a reaction kettle of the nutrient solution, wherein the inoculation amount of each bacteria liquid is as follows: 6% of Aspergillus niger bacterial liquid, 4% of penicillium oxalicum liquid, 2% of lactobacillus (streptococcus thermophilus) bacterial liquid, and 5% of bacillus megaterium bacterial liquid. Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-300 to-400 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 5:1, regulating the initial pH value of the solution to 6.0, slowly stirring through air at 30 ℃, adding gibberellin to ensure that the concentration reaches 1.0mg/L, and leaching for 72 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 2.5, and continuously leaching the ore for 48 hours.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 86.1 percent.
Example 2:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 is uniformly stirred with water according to the mass ratio of 1:10, and the mixture comprises 55g/L of sucrose, 25g/L, naCl of glucose and 0.8g/L, KCl 1.1.1 g/L, mgSO of glucose 4 ·7H 2 O 1.4g/L、NH 4 SO 4 1.9g/L、FeSO 4 ·7H 2 O 0.5g/L。
(2) Adding a composite phosphate-dissolving microbial liquid into a reaction kettle filled with a bacterial liquid nutrient solution, wherein the inoculation amount of each bacterial liquid is as follows: the inoculum size of the Aspergillus niger liquid is 7%, the inoculum size of the Penicillium oxalicum liquid is 5%, the inoculum size of the lactobacillus (streptococcus thermophilus) liquid is 4%, and the inoculum size of the Bacillus megaterium liquid is 3%. Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-300 to-400 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 5:1, regulating the initial pH value of the solution to 6.5, slowly stirring through air at 35 ℃, adding gibberellin to enable the concentration to reach 2.0mg/L, and leaching for 72 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 3.0, and continuously leaching the ore for 48 hours.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 98.1 percent.
Comparative example 2:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 is uniformly stirred with water according to the mass ratio of 1:10, and the mixture comprises 55g/L of sucrose, 25g/L, naCl of glucose and 0.8g/L, KCl 1.1.1 g/L, mgSO of glucose 4 ·7H 2 O 1.4g/L、NH 4 SO 4 1.9g/L、FeSO 4 ·7H 2 O 0.5g/L。
(2) Adding a composite phosphate-dissolving microbial liquid into a reaction kettle filled with a bacterial liquid nutrient solution, wherein the inoculation amount of each bacterial liquid is as follows: the inoculum size of the aspergillus niger liquid is 4 percent, the inoculum size of the penicillium oxalicum liquid is 1 percent, the inoculum size of the lactobacillus (streptococcus thermophilus) liquid is 6 percent, and the inoculum size of the bacillus megaterium liquid is 7 percent. Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-300 to-400 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 5:1, regulating the initial pH value of the solution to 6.5, slowly stirring through air at 35 ℃, adding gibberellin to enable the concentration to reach 2.0mg/L, and leaching for 72 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 3.0, and continuously leaching the ore for 48 hours.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 82.7 percent.
Example 3:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 is uniformly stirred with water according to the mass ratio of 1:10, and the mixture comprises 60g/L of sucrose, 24g/L, naCl of glucose 0.7g/L, KCl 1.0.0 g/L, mgSO 4 ·7H 2 O 1.5g/L、NH 4 SO 4 2.0g/L、FeSO 4 ·7H 2 O 0.7g/L。
(2) Adding a composite phosphate-dissolving microbial liquid into a reaction kettle filled with a bacterial liquid nutrient solution, wherein the inoculation amount of each bacterial liquid is as follows: the inoculum size of the Aspergillus niger liquid is 9%, the inoculum size of the Penicillium oxalicum liquid is 3%, the inoculum size of the lactobacillus (streptococcus thermophilus) liquid is 3%, and the inoculum size of the Bacillus megaterium liquid is 5%.Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-300 to-400 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 5:1, regulating the initial pH value of the solution to 6.8, slowly stirring through air at 37 ℃, adding gibberellin to enable the concentration to reach 4.0mg/L, and leaching for 72 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 3.5, and continuously leaching the ore for 48 hours.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 98.5 percent.
Comparative example 3:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 is uniformly stirred with water according to the mass ratio of 1:10, and the mixture comprises 60g/L of sucrose, 24g/L, naCl of glucose 0.7g/L, KCl 1.0.0 g/L, mgSO 4 ·7H 2 O 1.5g/L、NH 4 SO 4 2.0g/L、FeSO 4 ·7H 2 O 0.7g/L。
(2) Adding a composite phosphate-dissolving microbial liquid into a reaction kettle filled with a bacterial liquid nutrient solution, wherein the inoculation amount of each bacterial liquid is as follows: the inoculum size of the Aspergillus niger liquid is 9%, the inoculum size of the Penicillium oxalicum liquid is 3%, the inoculum size of the lactobacillus (streptococcus thermophilus) liquid is 3%, and the inoculum size of the Bacillus megaterium liquid is 5%. Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-100 to-200 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 6:1, regulating the initial pH value of the solution to 5.0, slowly stirring through air at 39 ℃, adding gibberellin to enable the concentration to reach 4.5mg/L, and leaching for 80 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 3.5, and continuously leaching the ore for 48 hours.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 94.1 percent.
Example 4:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mass ratio of the rice straw powder to the corn steep liquor dry powder isThe mixture with the mass ratio of 1:1 and water are evenly stirred according to the mass ratio of 1:10, and the sucrose is 58g/L, the glucose is 24g/L, naCl 0.9g/L, KCl 0.8.8 g/L, mgSO 4 ·7H 2 O 1.4g/L、NH 4 SO 4 1.6g/L、FeSO 4 ·7H 2 O 0.6g/L。
(2) Adding a composite phosphate-dissolving microbial liquid into a reaction kettle filled with a bacterial liquid nutrient solution, wherein the inoculation amount of each bacterial liquid is as follows: the inoculum size of the Aspergillus niger liquid is 9%, the inoculum size of the Penicillium oxalicum liquid is 3%, the inoculum size of the lactobacillus (streptococcus thermophilus) liquid is 3%, and the inoculum size of the Bacillus megaterium liquid is 5%. Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-300 to-400 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 5:1, regulating the initial pH value of the solution to 5.5, slowly stirring through air at 34 ℃, adding gibberellin to ensure that the concentration reaches 3.0mg/L, and leaching for 72 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 2.5, and continuously leaching the ore for 48 hours.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 98.8 percent.
Comparative example 4:
(1) The nutrient solution of the bacterial liquid is prepared according to the following formula: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 is uniformly stirred with water according to the mass ratio of 1:10, and the mixture comprises 58g/L of sucrose, 24g/L, naCl of glucose 0.9g/L, KCl 0.8.8 g/L, mgSO 4 ·7H 2 O 1.4g/L、NH 4 SO 4 1.6g/L、FeSO 4 ·7H 2 O 0.6g/L。
(2) Adding a composite phosphate-dissolving microbial liquid into a reaction kettle filled with a bacterial liquid nutrient solution, wherein the inoculation amount of each bacterial liquid is as follows: the inoculum size of the Aspergillus niger liquid is 9%, the inoculum size of the Penicillium oxalicum liquid is 3%, the inoculum size of the lactobacillus (streptococcus thermophilus) liquid is 3%, and the inoculum size of the Bacillus megaterium liquid is 5%. Wherein the concentration of each microbial liquid is 10 6 ～10 8 And each mL.
(3) Adding-300 to-400 mesh monazite concentrate particles into a reaction kettle according to a liquid-solid ratio of 5:1, regulating the initial pH value of the solution to 5.5, slowly stirring through air at 34 ℃, adding gibberellin to ensure that the concentration reaches 3.0mg/L, and leaching for 72 hours.
(4) Adding alpha-naphthylacetic acid into the reaction kettle to enable the pH value of the solution to be 2.5, and continuously leaching the ore for 55h.
According to the method, 50g of the monazite concentrate particles with the meshes of-300 to-400 are taken for decomposition, and the final monazite decomposition rate reaches 92.4 percent.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (6)
1. A method for decomposing monazite by utilizing composite microorganisms, which is characterized in that: the method comprises the following steps:
(1) Leaching in the first step: adding a composite phosphate-dissolving microbial liquid, monazite concentrate powder and gibberellin into a reaction kettle filled with a bacterial liquid nutrient solution, and adjusting the initial pH value of the solution to 5.5-7.0 for primary leaching; the conditions for the first leaching step are: introducing air at 30-37 ℃ and slowly stirring, leaching for 65-75 hours;
the compound phosphate-solubilizing microorganism comprises aspergillus niger, penicillium oxalicum, lactobacillus and bacillus megatherium;
the bacterial liquid nutrient solution comprises the following components: the mixture of the rice straw powder and the corn steep liquor dry powder with the mass ratio of 1:1 and water are uniformly stirred according to the mass ratio of 1:8-15, the sucrose is 55-60 g/L, the glucose is 20-25 g/L, naCl is 0.6-0.9 g/L, KCl 0.8.8-1.2 g/L, mgSO 4 ·7H 2 O 1.2～1.5g/L、NH 4 SO 4 1.7～2.0g/L、FeSO 4 ·7H 2 O 0.5～0.8g/L；
The concentration of the compound phosphate-dissolving microorganisms is 10 6 ～10 8 Inoculating each/mL, wherein the inoculum size of each bacterial liquid is 5-9% of Aspergillus niger inoculum size, 2-7% of Penicillium oxalicum inoculum size, 1-5% of lactobacillus inoculum size and 2-6% of bacillus megatherium inoculum size;
(2) Leaching in the second step: adding alpha-naphthylacetic acid into the reaction kettle to reduce the pH value of the solution to 2.5-3.5, and carrying out secondary leaching.
2. The method for decomposing a monazite by using composite microorganisms according to claim 1, wherein: in the step (1), the granularity of the monazite concentrate powder is-300 to-400 meshes.
3. The method for decomposing a monazite by using composite microorganisms according to claim 1, wherein: in the step (1), the addition amount of the monazite concentrate powder is 2:1-5:1.
4. The method for decomposing a monazite by using composite microorganisms according to claim 1, wherein: in the step (1), the final concentration of gibberellin is 1.0-4.0 mg/L.
5. The method for decomposing a monazite by using composite microorganisms according to claim 1, wherein: the second leaching step of the step (2) is carried out for 45-50 h.
6. The method for decomposing a monazite by using a composite microorganism according to any one of claims 1 to 5, wherein: the method comprises the following steps:
(1) Leaching in the first step: adding the composite phosphate-dissolving microbial bacteria solution into a reaction kettle filled with bacteria solution nutrient solution according to the following inoculation amount: 5 to 9 percent of Aspergillus niger bacterial liquid, 2 to 7 percent of Penicillium oxalicum bacterial liquid, 1 to 5 percent of lactic acid bacterial liquid, 2 to 6 percent of bacillus megaterium bacterial liquid, and 10 percent of each bacterial liquid 6 ～10 8 Inoculating at a concentration of individual/mL; adding monazite concentrate powder of-300 to-400 meshes according to a liquid-solid ratio of 2:1-5:1, adding gibberellin to enable the concentration of gibberellin in the solution to reach 1.0-4.0 mg/L, adjusting the initial pH value of the solution to be 5.5-7.0, introducing air at 30-37 ℃, slowly stirring, and leaching for 65-75 hours;
(2) Leaching in the second step: adding alpha-naphthylacetic acid into the reaction kettle to reduce the pH value of the solution to 2.5-3.5, and leaching for 45-50 h.
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