CN114231421A - Gibberella fujikuroi and fermentation method for producing GA3 - Google Patents
Gibberella fujikuroi and fermentation method for producing GA3 Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 42
- 230000004151 fermentation Effects 0.000 title claims abstract description 39
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 29
- 241000221778 Fusarium fujikuroi Species 0.000 title abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 19
- 241000223218 Fusarium Species 0.000 claims abstract description 17
- 238000004321 preservation Methods 0.000 claims abstract description 12
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 9
- 244000005700 microbiome Species 0.000 claims abstract description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 40
- 239000001963 growth medium Substances 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 20
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 20
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 20
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 20
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 20
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- 239000002609 medium Substances 0.000 claims description 16
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 10
- 244000105624 Arachis hypogaea Species 0.000 claims description 10
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 10
- 235000018262 Arachis monticola Nutrition 0.000 claims description 10
- 229920002261 Corn starch Polymers 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 108010073771 Soybean Proteins Proteins 0.000 claims description 10
- 229920002472 Starch Polymers 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 10
- 239000008120 corn starch Substances 0.000 claims description 10
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 10
- 239000011790 ferrous sulphate Substances 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 10
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 10
- 235000020232 peanut Nutrition 0.000 claims description 10
- 235000019710 soybean protein Nutrition 0.000 claims description 10
- 239000008107 starch Substances 0.000 claims description 10
- 235000019698 starch Nutrition 0.000 claims description 10
- 229960004793 sucrose Drugs 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 5
- 229930191978 Gibberellin Natural products 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000003448 gibberellin Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000005980 Gibberellic acid Substances 0.000 abstract description 5
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 3
- 239000005720 sucrose Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P27/00—Preparation of compounds containing a gibbane ring system, e.g. gibberellin
Abstract
The invention discloses a gibberella barnacantha and GA production3The fermentation method of (1), the Gibberella fujikuroi strain is preserved in common microorganisms of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC No. 23265. The gibberellic acid strain of the invention is used for producing GA3High yield, impurity GA1The amount is small.
Description
Technical Field
The invention relates to a gibberella barnacantha and GA3And a GA3Production method of (2) and a production method of GA3The method of fermentation of (1).
Background
Gibberellic acid is a secondary metabolite obtained by fermentation and metabolism of a kind of gibberella, and 116 kinds of gibberellic acid are isolated, identified and named, among which GA is the most widely used in agriculture3Plays a great role in agricultural production in China.
GA3Has very high plant regulating activity and obvious regulating effect on the growth and development of various crops. In the north, GA3The gibberellic acid solution with a certain concentration is sprayed in the flowering phase, so that the fruit setting rate of fruit trees can be effectively improved, the fruit growth and development are promoted, and the fruit yield is improved by about 20-30%. In the south, gibberellic acid is mainly applied to hybrid rice seed production, and male and female parents of rice are regulated to bloom at the same time, so that the pollination rate of the female parents is greatly improved, the yield of hybrid rice seeds is further improved, the production cost of the seeds is reduced, and the burden of farmers is reduced.
Industrial production of GA by microbial fermentation3However, high levels of GA produced directly by fermentation suitable for industrial production3The strain and the technical method of the product are not reported.
Disclosure of Invention
In view of the above problems, the present invention provides a gibberellin, the gibberellin GA3High yield, impurity GA1The amount is small.
The gibberella barnachos is deposited in common microbe of China Committee for culture Collection of microorganisms with the preservation number of CGMCC No. 23265.
In one or more specific embodiments of the present application, the gibberella bardawil is GA-producing3The bacterium of (1).
Based on the gibberella barnacne, the application also provides a GA3。
GA3The GA3Is prepared from the composition containing the gibberella barnacii.
Based on the gibberella barnacne, the application also provides a GA3The production method of (1).
GA3The production method of (1), wherein the raw material for the production method comprises the above-mentioned Gibberella fujikuroi strain.
Based on the gibberella barnachos, the application also provides a method for producing GA3The method of fermentation of (1).
Production GA3The fermentation method of (2), comprising the steps of:
culturing the red-eared mildew with the preservation number of CGMCC No.23265 with a first culture medium to obtain a seed solution;
secondly, inoculating the seed liquid to a second culture medium;
③ fermenting to obtain the GA3The fermentation broth of (1).
In one or more specific embodiments of the present application, the first medium is composed in amounts of: 1-5% of corn starch, 0.1-5% of glucose, 0.1-5% of soybean cake powder, 0.01-0.5% of monopotassium phosphate, 0.01-0.5% of magnesium sulfate, 0.01-0.5% of ammonium sulfate and the balance of water; the second culture medium comprises the following components in percentage by weight: 1-10% of starch, 0.1-5% of cane sugar, 0.1-5% of peanut cake powder, 0.1-5% of soybean protein, 0.01-0.5% of monopotassium phosphate, 0.01-0.5% of magnesium sulfate, 0.001-0.005% of ferrous sulfate and the balance of water; wherein,% is mass percent.
In one or more specific embodiments of the present application, the first medium is composed in amounts of: 2-3% of corn starch, 1-2% of glucose, 1-2% of soybean cake powder, 0.1-0.3% of monopotassium phosphate, 0.05-0.1% of magnesium sulfate, 0.05-0.1% of ammonium sulfate and the balance of water; the second culture medium comprises the following components in percentage by weight: 5-7% of starch, 1-2% of cane sugar, 1-2% of peanut cake powder, 1-2% of soybean protein, 0.1-0.2% of monopotassium phosphate, 0.1-0.2% of magnesium sulfate, 0.001-0.005% of ferrous sulfate and the balance of water; wherein,% is mass percent.
In one or more specific embodiments of the present application, in said (r), the gibberella alvanica with the accession number of CGMCC No. 23265: the volume ratio of the first culture medium is 0.03-0.08: 100.
in one or more specific embodiments of the present application, the inoculation amount of the seed solution inoculated to the second culture medium is 8-10%, wherein% is mass percent.
Production of GA based on the above3The invention also provides a GA3The production method of (1).
GA3Characterized in that the production process comprises the above-mentioned production of GA3Fermentation method and production of GA3The purification method of (1).
In one or more specific embodiments of the present application, the production of GA3The purification method of (3) is a concentration method.
The invention principle and the beneficial effects are as follows:
application of Gibberella fujikuroi in fermentation production of GA-containing bacteria3The fermentation broth of (1), shaking flask fermentation GA3The yield is 1500-1700 ppm, and the impurity GA1The yield is 80-120 ppm.
Biological preservation Instructions
The deposited bacteria are classified and named as Gibberella fujikuroi, deposited in China general microbiological culture Collection center (address: Beijing, West Lu No. 1 of the morning area, Xihe No. 1 of the national academy of sciences, Japan, the postal code: 1000101) at 26 days 09.26.2021, and the deposited number is CGMCC No.23265, used for fermentation production of GA3。
Drawings
FIG. 1 is a macroscopic morphological diagram of the present application, Gibberella fujikuroi;
FIG. 2 is a microscopic morphology of the present application, Gibberella fujikuroi.
Detailed Description
The invention will be further explained with reference to the drawings.
Example 1
The method comprises the steps of separating an original strain of gibberella from corn by a Tetrachuan python Fusheng science and technology Limited liability company, digging a single colony of the gibberella to form a first culture medium with the size of 1cm multiplied by 1cm for shake flask culture, culturing at 30 DEG and 200rpm for 1 day, then dibbling a potato juice flat plate paved with a microporous filter membrane, culturing at 30 DEG for 2 days until white hyphae grow on the filter membrane, scraping the hyphae with sterile water to prepare hypha suspension, wherein the hypha suspension prepared by the method does not contain culture medium components, and does not influence the subsequent mutagenesis operation.
Irradiating the hypha suspension with a 15w ultraviolet lamp at a distance of 30cm for 30 seconds, and adding an equal amount of 8% lithium chloride solution to react for 3 hours to obtain a mutant gibberella suspension.
And (3) performing mixed culture on the hypha suspension after mutagenesis by using a second culture medium, performing culture at 30 degrees and 200rpm for 7 days, spraying fermentation liquor on stem leaves of the Chinese cabbage in the growth period, continuing to culture for 10 days, picking off the stem leaves which obviously grow excessively, paving the stem leaves on a potato juice culture medium, performing culture at 30 degrees for about 5 days, and picking out the gibberella zeae growing on a flat plate to perform separation and purification to obtain the gibberella barnachosii with the preservation number of CGMCC No. 23265.
The Fusarium canescens of CGMCC No.23265 does not produce spores, hypha is in large-scale net structure under microscope, has branching and separation, and is white on potato juice culture medium, grows vigorously, is velvet in texture, and is orange on the reverse side of colony, as shown in figure 1-2.
In this example, the first medium comprises: 2% of corn starch, 1% of glucose, 1% of soybean cake powder, 0.1% of monopotassium phosphate, 0.05% of magnesium sulfate, 0.05% of ammonium sulfate and the balance of water.
In this example, the second medium comprises: 5% of starch, 1% of sucrose, 1% of peanut cake powder, 1% of soybean protein, 0.1% of monopotassium phosphate, 0.1% of magnesium sulfate, 0.001% of ferrous sulfate and the balance of water.
Example 2
70ul of the gibberiella barnacii with the preservation number of CGMCC No.23265 is inoculated into 200ml of a first culture medium and is cultured for 3 days in a shake flask to obtain a seed solution, and the culture condition is 30 degrees and 200 rpm.
② the seed liquid is inoculated to the second culture medium with 8 percent of inoculation amount (8 percent of inoculation amount is that the seed liquid amount is 8 percent of the second culture medium amount,% is volume percent).
And thirdly, shake flask fermentation culture for 7d to obtain fermentation liquor, and fermentation is carried out under the conditions of 30 degrees and 200 rpm.
And fourthly, detecting the fermentation liquor, wherein the detection result is shown in the following table 1.
In this example, the first medium comprises: 2% of corn starch, 1% of glucose, 1% of soybean cake powder, 0.1% of monopotassium phosphate, 0.05% of magnesium sulfate, 0.05% of ammonium sulfate and the balance of water.
In this example, the second medium comprises: 5% of starch, 1% of sucrose, 1% of peanut cake powder, 1% of soybean protein, 0.1% of monopotassium phosphate, 0.1% of magnesium sulfate, 0.001% of ferrous sulfate and the balance of water.
Example 3
70ul of the gibberiella barnacii with the preservation number of CGMCC No.23265 is inoculated into 200ml of a first culture medium and is cultured for 3 days in a shake flask to obtain a seed solution, and the culture condition is 30 degrees and 200 rpm.
② the seed liquid is inoculated to the second culture medium with 8 percent of inoculation amount.
And thirdly, shake flask fermentation culture for 7d to obtain fermentation liquor, and fermentation is carried out under the conditions of 30 degrees and 200 rpm.
And fourthly, detecting the fermentation liquor, wherein the detection result is shown in the following table 1.
In this example, the first medium comprises: 2.53 percent of corn starch, 1.5 percent of glucose, 1.5 percent of soybean cake powder, 0.2 percent of monopotassium phosphate, 0.08 percent of magnesium sulfate, 0.08 percent of ammonium sulfate and the balance of water.
In this example, the second medium comprises: 6% of starch, 1.5% of sucrose, 1.5% of peanut cake powder, 1.5% of soybean protein, 0.15% of monopotassium phosphate, 0.15% of magnesium sulfate, 0.003% of ferrous sulfate and the balance of water.
Example 4
70ul of the gibberiella barnacii with the preservation number of CGMCC No.23265 is inoculated into 200ml of a first culture medium and is cultured for 3 days in a shake flask to obtain a seed solution, and the culture condition is 30 degrees and 200 rpm.
② the seed liquid is inoculated to the second culture medium with 8 percent of inoculation amount.
And thirdly, shake flask fermentation culture for 7d to obtain fermentation liquor, and fermentation is carried out under the conditions of 30 degrees and 200 rpm.
And fourthly, detecting the fermentation liquor, wherein the detection result is shown in the following table 1.
In this example, the first medium comprises: 3% of corn starch, 2% of glucose, 2% of soybean cake powder, 0.3% of monopotassium phosphate, 0.1% of magnesium sulfate, 0.1% of ammonium sulfate and the balance of water.
In this example, the second medium comprises: 7% of starch, 2% of sucrose, 2% of peanut cake powder, 2% of soybean protein, 0.2% of monopotassium phosphate, 0.2% of magnesium sulfate, 0.005% of ferrous sulfate and the balance of water.
Experiment 5
70ul of the gibberiella barnacii with the preservation number of CGMCC No.23265 is inoculated into 200ml of a first culture medium and is cultured for 3 days in a shake flask to obtain a seed solution, and the culture condition is 30 degrees and 200 rpm.
② inoculating the seed liquid into a second culture medium with 9 percent of inoculation amount.
And thirdly, shake flask fermentation culture for 7d to obtain fermentation liquor, and fermentation is carried out under the conditions of 30 degrees and 200 rpm.
And fourthly, detecting the fermentation liquor, wherein the detection result is shown in the following table 1.
In this example, the first medium comprises: 3% of corn starch, 2% of glucose, 2% of soybean cake powder, 0.3% of monopotassium phosphate, 0.1% of magnesium sulfate, 0.1% of ammonium sulfate and the balance of water.
In this example, the second medium comprises: 7% of starch, 2% of sucrose, 2% of peanut cake powder, 2% of soybean protein, 0.2% of monopotassium phosphate, 0.2% of magnesium sulfate, 0.005% of ferrous sulfate and the balance of water.
Experiment 6
70ul of the gibberiella barnacii with the preservation number of CGMCC No.23265 is inoculated into 200ml of a first culture medium and is cultured for 3 days in a shake flask to obtain a seed solution, and the culture condition is 30 degrees and 200 rpm.
② the seed liquid is inoculated to the second culture medium with 10 percent of inoculation amount.
And thirdly, shake flask fermentation culture for 7d to obtain fermentation liquor, and fermentation is carried out under the conditions of 30 degrees and 200 rpm.
And fourthly, detecting the fermentation liquor, wherein the detection result is shown in the following table 1.
In this example, the first medium comprises: 3% of corn starch, 2% of glucose, 2% of soybean cake powder, 0.3% of monopotassium phosphate, 0.1% of magnesium sulfate, 0.1% of ammonium sulfate and the balance of water.
In this example, the second medium comprises: 7% of starch, 2% of sucrose, 2% of peanut cake powder, 2% of soybean protein, 0.2% of monopotassium phosphate, 0.2% of magnesium sulfate, 0.005% of ferrous sulfate and the balance of water.
TABLE 1
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The gibberella barnachos is deposited in common microbe of China Committee for culture Collection of microorganisms with the preservation number of CGMCC No. 23265.
2. The gibberellin gambieri of claim 1, wherein the gibberellin gambieri is GA production3The bacterium of (1).
3. GA3Characterized in that the GA3Is prepared from the composition of red-eyed plant as in any claim 1-2.
4. GA3Characterized in that the source of the production processThe feed comprises the gibberella barnacii strain of any one of claims 1-2.
5. Production GA3The fermentation method of (2), comprising the steps of:
culturing the red-eared mildew with the preservation number of CGMCC No.23265 with a first culture medium to obtain a seed solution;
secondly, inoculating the seed liquid to a second culture medium;
③ fermenting to obtain the GA3The fermentation broth of (1).
6. Production of GA according to claim 53The fermentation method of (2), characterized in that the first medium comprises: 1-5% of corn starch, 0.1-5% of glucose, 0.1-5% of soybean cake powder, 0.01-0.5% of monopotassium phosphate, 0.01-0.5% of magnesium sulfate, 0.01-0.5% of ammonium sulfate and the balance of water; the second culture medium comprises the following components in percentage by weight: 1-10% of starch, 0.1-5% of cane sugar, 0.1-5% of peanut cake powder, 0.1-5% of soybean protein, 0.01-0.5% of monopotassium phosphate, 0.01-0.5% of magnesium sulfate, 0.001-0.005% of ferrous sulfate and the balance of water; wherein,% is mass percent.
7. Production of GA according to claim 63The fermentation method of (2), characterized in that the first medium comprises: 2-3% of corn starch, 1-2% of glucose, 1-2% of soybean cake powder, 0.1-0.3% of monopotassium phosphate, 0.05-0.1% of magnesium sulfate, 0.05-0.1% of ammonium sulfate and the balance of water; the second culture medium comprises the following components in percentage by weight: 5-7% of starch, 1-2% of cane sugar, 1-2% of peanut cake powder, 1-2% of soybean protein, 0.1-0.2% of monopotassium phosphate, 0.1-0.2% of magnesium sulfate, 0.001-0.005% of ferrous sulfate and the balance of water; wherein,% is mass percent.
8. Production of GA according to any of claims 5-73The fermentation method of (1), wherein the number of deposits is CGMCC No. 23265: first culture mediumThe volume ratio is 0.03-0.08: 100, and/or
In the second step, the inoculation amount of the seed liquid inoculated to the second culture medium is 8-10 percent; wherein,% is volume percentage.
9. GA3The production process of (1), which comprises producing GA as described in any one of claims 5 to 83Fermentation method and production of GA3The purification method of (1).
10. The GA of claim 93Characterized in that the production of GA3The purification method of (3) is a concentration method.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002055725A2 (en) * | 2000-11-14 | 2002-07-18 | Phibro-Tech, Inc., Doing Business As Agtrol International | Novel nucleic acids, methods and transformed cells for the modulation of gibberellin production |
| ES2221786A1 (en) * | 2002-12-20 | 2005-01-01 | Universidad De Sevilla | Gibberella Fujikuroi wild strains fermentation comprises production of Gibberellins GA1 and GA3 by inoculation and solvent extraction |
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