CN114225986A - Liquid transportation system for micro-fluidic chip - Google Patents
Liquid transportation system for micro-fluidic chip Download PDFInfo
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- CN114225986A CN114225986A CN202210103852.8A CN202210103852A CN114225986A CN 114225986 A CN114225986 A CN 114225986A CN 202210103852 A CN202210103852 A CN 202210103852A CN 114225986 A CN114225986 A CN 114225986A
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- 239000007788 liquid Substances 0.000 title claims abstract description 79
- 230000005540 biological transmission Effects 0.000 claims abstract description 14
- 238000002347 injection Methods 0.000 claims abstract description 5
- 239000007924 injection Substances 0.000 claims abstract description 5
- 239000012530 fluid Substances 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 4
- 230000002209 hydrophobic effect Effects 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 9
- 239000000126 substance Substances 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 239000012491 analyte Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
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- 230000001351 cycling effect Effects 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000004094 preconcentration Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Structures Of Non-Positive Displacement Pumps (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a liquid transportation system of a microfluidic chip, which comprises: a microfluidic body, the liquid injection port to be measured; a microfluidic pipeline is arranged in the microfluidic main body; the micro-fluidic chip is provided with at least one micro pump which is correspondingly coupled in the micro-fluidic pipeline to control the flow rate of the liquid; the sensor comprises a sensor chip, a transmission medium with one end connected with the sensor chip and a card reader with the other end connected with the transmission medium; a power source coupled to the micro-pump configured to power a microfluidic chip liquid transport system. According to the invention, the liquid transportation impeller set and the driving impeller set are arranged in the microfluidic chip, the external magnetic field is used for driving the magnet, so that the flow velocity of liquid in the chip can be accurately controlled, the liquid to be tested circularly flows through the sensor under the cooperation of a specific sensor so as to meet the requirements of chemical or biological reactions, the rapid test effect can be achieved, and the low-concentration quantitative detection can be carried out.
Description
Technical Field
The invention relates to the technical field of microfluid, in particular to a microfluidic chip liquid transportation system.
Background
Microfluidic devices utilize the physical and chemical properties of liquids and gases on a microscale. Microfluidic devices offer several advantages over systems of conventional size. Microfluidics allows the use of relatively small volumes of samples, chemicals and reagents for output, very important screening, diagnostic and research applications, and is therefore widely used in the chemical and biological paradigm.
Direct control of flow is generally a larger system, with the advantage that the system can control flow and velocity accurately, and can handle larger volumes of liquid. The defects that the device is only suitable for laboratories, the consumption of consumables is large, the operation is complicated, trained technicians are usually required to operate, and the device is not suitable for outdoor field application.
In another alternative, the card reading system provides power to mount the liquid driving unit on the microfluidic chip, which has the advantages of simple operation, concentrated consumables on the chip, and capability of cooperating with the sensor, and the application range can include but is not limited to environmental pollution detection, biomarkers, and the like.
The prior art includes various methods such as pneumatic, electrostatic, electrophoretic, MEMS, diaphragm pump, etc., but they all have a common disadvantage in that these systems are not necessarily satisfactory for handling when the analyte concentration is too low and requires a certain reaction time. For example, some diseases have biomarker concentrations of only tens of molecules or less within 100uL, requiring pre-concentration procedures or long cycling tests to increase the detection limit, in which case the test may need to be returned to the laboratory for processing.
Therefore, a system that can handle a relatively large capacity (up to milli-upgrade) and can implement the related functions is urgent.
Disclosure of Invention
In view of the above, the present invention provides a microfluidic chip liquid transportation system, which is configured to control a liquid flow rate.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention discloses a liquid transportation system of a microfluidic chip, which comprises:
the microfluid injection port is arranged on the microfluid main body; a microfluidic pipeline is arranged in the microfluidic main body;
at least one micropump correspondingly coupled in the microfluidic pipeline to control the flow rate of the liquid;
the sensor comprises a sensor chip of an acquisition point and a transmission medium, wherein one end of the transmission medium is connected with the acquisition point of the sensor chip;
a power source coupled to the micro-pump configured to power a microfluidic chip liquid transport system.
Further, the micro pump includes: a micro pump shaft; at least one driving impeller set configured to couple to the micro pump shaft to drive the micro pump shaft to rotate.
Further, the micro pump further includes: at least one liquid transport impeller assembly configured to be coupled to the micropump shaft for rotation therewith.
Further, the driving impeller set includes: one end of the micro impeller blade is fastened on the micro pump shaft, and the other end of the micro impeller blade is provided with a magnet to drive the micro pump shaft to rotate.
Furthermore, the micro impeller blade is made of hydrophobic or hydrophilic materials.
Furthermore, at least one groove is arranged on the microfluidic pipeline in the microfluidic main body and used for fixing the micropump.
Furthermore, a magnet is arranged on the driving impeller set.
Further, the liquid transport impeller assembly comprises:
and one end of the micro impeller blade is fastened on the micro pump shaft, so that the micro pump shaft rotates along with the rotation of the micro impeller blade.
Furthermore, the microflow pipeline is divided into at least two sections of flow channels with different cross-sectional areas.
Further, the diameter of the micro impeller blade is 1-10 mm.
Further, the other end of the transmission medium is connected to a card reader and used for reading the information to be detected collected and processed by the sensor
The invention can accurately control the flow rate of liquid in the chip by arranging the liquid transportation impeller set and the driving impeller set in the microfluidic chip and using the external magnetic field to drive the magnet, and the liquid to be detected circularly flows through the sensor under the coordination of the specific sensor so as to meet the requirements of chemical or biological reaction and make quantitative detection of low concentration. The whole system can change some tests needing to be purified for a plurality of times in a laboratory into portable outdoor use.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a first schematic structural diagram of a microfluidic chip liquid transportation system according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a part of a microfluidic chip liquid transportation system provided by an embodiment of the present invention, the part having a driving impeller set and a liquid transportation impeller set;
fig. 3 is a schematic structural diagram of a microfluidic chip liquid transportation system provided by an embodiment of the present invention, in which only a driving impeller set and a liquid transportation impeller set are partially integrated together;
fig. 4 is a schematic view of a driving impeller set of a microfluidic chip liquid transportation system according to an embodiment of the present invention.
The system comprises a micro-fluid main body 1, a micro-pump 2, a sensor 3, a power source 4, a liquid injection port to be detected 101, a micro-fluid pipeline 102, a groove 1021, a micro-pump shaft 201, a driving impeller set 202, a liquid transportation impeller set 203, a magnet 2021, a micro-impeller blade 2022, a sensor chip 301, a transmission medium 302 and a card reader 303.
Detailed Description
In view of the above, the core of the present invention is to provide a microfluidic chip liquid transportation system, so as to control the liquid flow rate by providing the microfluidic chip liquid transportation system.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, fig. 2, fig. 3 and fig. 4, an embodiment of the present invention discloses a microfluidic chip liquid transportation system, including:
the device comprises a microfluid main body 1, wherein a microfluid liquid injection port 101 to be detected is arranged on the microfluid main body; a microfluidic pipeline 102 is arranged in the microfluidic main body 1;
at least one micro pump 2, wherein the micro pump 2 is correspondingly coupled in the micro flow pipeline 102 to control the flow rate of the liquid;
the micro pump 2 is fixed on the micro fluid body 1 in a packaging mode in the invention. It should be understood that the micro-pump 2 may be secured by other means than encapsulation.
The sensor 3, the sensor 3 comprises a sensor chip mounted on a sensor chip 301 and a transmission medium 302 with one end connected to the sensor chip 301 and a card reader 303 with the other end of the transmission medium 302 connected to the outside of the microfluid. The sensor chip 301 is provided with at least one collection point for collecting liquid to be measured flowing through the collection point, so as to transmit data to the sensor chip 301, and after being processed by the sensor chip 301, the sensor chip is connected with the card reader 303 through a transmission medium to read related data.
It should be understood that in one embodiment of the present invention, the sensor chip 301 and the transmission medium 302 of the sensor 3 are contacts and leads in a general sense. It can be any type of sensor that detects any particular material, and can also include a radio frequency module for measurement and data transmission. The main function of the microfluidic chip liquid transportation system is that a small amount of liquid can be used to achieve the effect of rapid cycle testing. When a liquid flows across the sensor surface, i.e., across the sensor chip 301, the analyte reacts with the sensor of the sensor chip 301. Whether biological or chemical detection, the analyte is pushed onto the sensor surface and a signal is generated and then measured by connecting to a card reader 303 through a transmission medium 302.
A power source 4, the power source 4 being coupled to the micro-pump 2 and configured to power the microfluidic chip liquid transport system; the power source 4 of the invention is a magnet or a coil arranged in the card reader to provide a power source for driving the micropump.
The power source 4 for the flow of liquid in the microfluidic device can be broadly divided into two major categories, active and passive. Passive methods generally refer to microfluidics without any external actuators, fields, or power sources. The motive force employs osmosis, capillary action, surface tension, pressure, gravity driven flow, hydrostatic flow and vacuum to effect fluid flow. Active methods require external power to drive the fluid to operate, and are also classified into 2 categories, which are energy only and direct flow control. The embodiment of the invention adopts an active method power source. I.e. by electromagnetic forces.
In a preferred embodiment, the micro-pump 2 comprises: a micropump shaft 201; at least one driving impeller set 202, wherein the driving impeller set 202 is configured to be coupled to the micropump shaft 201 to drive the micropump shaft 201 to rotate.
In a preferred embodiment, the micro-pump 2 further comprises: at least one liquid transport impeller set 203, the liquid transport impeller set 203 configured to couple to the micro pump shaft 201 so as to rotate with the micro pump shaft 201.
It is expected that the micro pump 2 functions relatively simply, i.e. as a water pump to drive the liquid in the micro fluid. It can be understood that the micro pump 2 pushes the liquid back into the micro fluid after passing through the sensor, i.e. the function of circulating the micro fluid. The micro pump 2 is used for circularly conveying liquid in the micro fluid. The technical scheme of the invention is that the two driving impeller sets 202 are driven by external magnetic force to complete liquid conveying from the driving impeller sets 202.
As shown in fig. 3, it should be understood that when the micro-pump 2 is provided with only one layer of micro-impeller blades 2022, then the micro-impeller blades 2022 are the driving impeller assembly 202, and have a fluid transporting function in addition to the function of driving the impeller to rotate.
As shown in fig. 2, it should be understood that when the micro-pump 2 is provided with multiple layers of micro-impeller blades 2022, then the multiple layers of micro-impeller blades 2022 may be divided into a driving impeller set 202 and a liquid transport impeller set 203.
In a preferred embodiment, the micro-impeller blades 2022 of the driving impeller assembly 202 are provided with magnets 2021.
In a preferred embodiment, the drive impeller assembly 202 includes: one end of the micro-impeller 2022 is fastened to the micro-pump shaft 201, and the other end of the micro-impeller 2022 is provided with a magnet 2021 to drive the micro-pump shaft 201 to rotate.
In a preferred embodiment, the micro-impeller assembly 202 is equipped with three or more micro-impeller blades 2022, and the card reader can use magnetic field transformation to monitor impeller drive and speed.
In a preferred embodiment, the micro-impeller 202 is made of hydrophobic or hydrophilic material and does not react with the liquid to be measured.
In a preferred embodiment, a groove 1021 is provided in the microfluidic channel 102 in the microfluidic body for holding the micropump 2.
In a preferred embodiment, the microfluidic channel 102 is divided into at least two flow paths of different cross-sectional areas.
In a preferred embodiment, the diameter of the micro-impeller 203 is 1-10 mm.
In a preferred embodiment, the cross-sectional area of the micro-flow pipeline 102 at the front section of the micro-pump 2 of the micro-flow pipeline 102 along the flowing direction of the liquid is larger than that of the micro-flow pipeline 102 at the rear section of the micro-pump 2, so as to realize that the liquid to be tested flows rapidly under the action of the micro-pump 2, and realize that the more obvious liquid to be tested is ensured that the target detection object can be captured by the sensor to the maximum extent after all the liquid is operated for many times by combining the pipelines from large to small, thereby achieving the effect of rapid cycle test.
In a preferred embodiment, the liquid transport impeller assembly 203 comprises: a micro-impeller 2022 having one end fastened to the micro-pump shaft 201, the micro-impeller 2022 being rotated by the rotation of the micro-pump shaft 201
In some embodiments of the present invention, the microfluidic body is a container, and the liquid to be measured is sent to the sensor for detection. This has the advantage that the entire test can be performed using a very small amount of liquid. The micro pump 2 of the invention is used for circularly conveying liquid in the micro fluid. Since there are some analytes with very low concentrations, such as some mRNAs or proteins, and only a few to tens of molecules in a 100. mu.l liquid, detection methods are not generally available, and PCR is used to replicate the molecules and amplify the signal. But this requires a long time in the laboratory to complete the test. The technical scheme of the invention can achieve the effect of rapid cycle test and does not need to be carried out in a laboratory.
According to the invention, the liquid transportation impeller set 203 and the driving impeller set 202 are arranged in the micro-fluidic chip, the external magnetic field driving magnet 2021 can be used for accurately controlling the flow rate of liquid in the chip, and under the cooperation of a specific sensor, liquid to be detected circularly flows through the sensor to meet the requirements of chemical or biological reactions, so that low-concentration quantitative detection can be carried out. The whole system can change some tests needing to be purified for a plurality of times in a laboratory into portable outdoor use.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A microfluidic chip liquid transport system, comprising:
the device comprises a microfluid main body (1), wherein a microfluid injection port (101) to be detected is arranged on the microfluid main body (1); a micro-flow pipeline (102) is arranged in the micro-fluid main body (1);
at least one micro pump (2), wherein the micro pump (2) is correspondingly coupled in the micro flow pipeline (102) to control the liquid flow rate;
the sensor (3), the said sensor (3) includes the sensor chip (301), one end connects to the transmission medium (302) of the sensor chip (301);
a power source (4), the power source (4) coupled to the micro-pump (2) configured to power a microfluidic chip liquid transport system.
2. Microfluidic chip liquid transport system according to claim 1, characterized in that the micropump (2) comprises:
a micropump shaft (201);
at least one driving impeller set (202), wherein the driving impeller set (202) is configured to be coupled to the micro pump shaft (201) to drive the micro pump shaft (201) to rotate.
3. The microfluidic chip liquid transport system of claim 2, wherein the micropump (2) further comprises:
at least one liquid transport impeller assembly (203), the liquid transport impeller assembly (203) configured to be coupled to the micropump shaft (201) so as to rotate with the rotation of the micropump shaft (201).
4. The microfluidic chip liquid transport system of claim 2, wherein the drive impeller set (202) comprises:
one end of the micro-impeller blade (2022) is fastened on the micro-pump shaft (201), and the other end of the micro-impeller blade (2022) is provided with a magnet (2021) to drive the micro-pump shaft (201) to rotate.
5. The microfluidic chip liquid transport system of claim 4, wherein the micro impeller blades (2022) are made of hydrophobic or hydrophilic material.
6. Microfluidic chip liquid transport system according to claim 1, wherein the microfluidic channel (102) in the microfluidic body is provided with at least one groove (1021) for holding the micropump (2).
7. The microfluidic chip liquid transport system of claim 1, wherein the microfluidic channel (102) is divided into at least two flow paths having different cross-sectional areas.
8. The microfluidic chip liquid transport system of claim 1, wherein the micro pump (2) is correspondingly packaged in the microfluidic pipeline (102) to control a liquid flow rate.
9. The microfluidic chip liquid transport system of claim 3, wherein the liquid transport impeller assembly (203) comprises:
a micro-impeller blade (2022) having one end fastened to the micro-pump shaft (201) such that the micro-pump shaft (201) rotates with the rotation of the micro-impeller blade (2022).
10. The microfluidic chip liquid transportation system according to claim 1, wherein the other end of the transmission medium (302) is connected to a card reader (303) for reading the information to be measured collected and processed by the sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210103852.8A CN114225986A (en) | 2022-01-28 | 2022-01-28 | Liquid transportation system for micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210103852.8A CN114225986A (en) | 2022-01-28 | 2022-01-28 | Liquid transportation system for micro-fluidic chip |
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| Publication Number | Publication Date |
|---|---|
| CN114225986A true CN114225986A (en) | 2022-03-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210103852.8A Pending CN114225986A (en) | 2022-01-28 | 2022-01-28 | Liquid transportation system for micro-fluidic chip |
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| Country | Link |
|---|---|
| CN (1) | CN114225986A (en) |
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- 2022-01-28 CN CN202210103852.8A patent/CN114225986A/en active Pending
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