CN114224868A - Double-layer exosome with core-shell structure and preparation method thereof - Google Patents
Double-layer exosome with core-shell structure and preparation method thereof Download PDFInfo
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 107
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- 239000011258 core-shell material Substances 0.000 title claims description 16
- 238000000034 method Methods 0.000 claims abstract 9
- 229920001661 Chitosan Polymers 0.000 claims description 55
- 239000002105 nanoparticle Substances 0.000 claims description 35
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 19
- 210000002540 macrophage Anatomy 0.000 claims description 18
- 210000004027 cell Anatomy 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 16
- 230000000968 intestinal effect Effects 0.000 claims description 14
- 239000004094 surface-active agent Substances 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 230000007910 cell fusion Effects 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 8
- 239000007762 w/o emulsion Substances 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 6
- IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 claims description 4
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 claims description 4
- 229920004890 Triton X-100 Polymers 0.000 claims description 4
- 239000013504 Triton X-100 Substances 0.000 claims description 4
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 4
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002202 Polyethylene glycol Substances 0.000 claims description 2
- 235000019864 coconut oil Nutrition 0.000 claims description 2
- 239000003240 coconut oil Substances 0.000 claims description 2
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 2
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 claims description 2
- 239000000194 fatty acid Substances 0.000 claims description 2
- 229930195729 fatty acid Natural products 0.000 claims description 2
- 150000004665 fatty acids Chemical class 0.000 claims description 2
- 150000002191 fatty alcohols Chemical class 0.000 claims description 2
- 238000000703 high-speed centrifugation Methods 0.000 claims description 2
- 238000000464 low-speed centrifugation Methods 0.000 claims description 2
- ZPIRTVJRHUMMOI-UHFFFAOYSA-N octoxybenzene Chemical compound CCCCCCCCOC1=CC=CC=C1 ZPIRTVJRHUMMOI-UHFFFAOYSA-N 0.000 claims description 2
- ONJQDTZCDSESIW-UHFFFAOYSA-N polidocanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO ONJQDTZCDSESIW-UHFFFAOYSA-N 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 239000001587 sorbitan monostearate Substances 0.000 claims description 2
- 235000011076 sorbitan monostearate Nutrition 0.000 claims description 2
- 229940035048 sorbitan monostearate Drugs 0.000 claims description 2
- 206010028980 Neoplasm Diseases 0.000 description 6
- AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 3
- 239000012528 membrane Substances 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 239000000232 Lipid Bilayer Substances 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 229940009456 adriamycin Drugs 0.000 description 1
- 238000012382 advanced drug delivery Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/5176—Compounds of unknown constitution, e.g. material from plants or animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5161—Polysaccharides, e.g. alginate, chitosan, cellulose derivatives; Cyclodextrin
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
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- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
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Abstract
The invention provides an exosome providing multiple sources and a preparation method thereof, in particular to a double-layer exosome and a preparation method thereof. The method has the advantages that the exosomes with multiple sources can be simultaneously realized, and the functions of the exosomes are increased. The preparation method has the advantages of simple preparation process, easy control of reaction, good stability and industrialization.
Description
Technical Field
The invention relates to the field of biology, relates to an exosome with multiple sources and a preparation method thereof, and particularly relates to a double-layer exosome with a core-shell structure and a preparation method thereof.
Background
Exosomes are tiny membrane vesicles secreted by most cells in the body, with lipid bilayer membranes, approximately 30-150 nm in diameter. Exosomes are widely existed and distributed in various body fluids, carry and transmit important signal molecules, form a brand-new cell-cell information transmission system, influence the physiological state of cells and are closely related to the occurrence and the progress of various diseases. Therefore, exosomes are often used as a tool for genetic information transfer. In addition, exosomes have become the next generation of endogenous nanomaterials for advanced drug delivery and therapy. For example, there are researchers who developed exosome-biomimetic nanoparticles that can efficiently deliver drugs to kill tumors. The research develops a biocompatible tumor cell-exocytosis exosome sheath PSiNPs (silicon nano material) as a drug carrier for targeted cancer chemotherapy. When tumor cells are incubated with PSiNP loaded with adriamycin (DOX), the tumor cells can secrete exosomes loaded with DOX @ PSiNPs, and the exosomes are used for targeted drug delivery of tumor stem cells and have good capacity of resisting tumors and killing the tumor stem cells. However, the exosomes studied at present are all single-source, and how to prepare exosomes from multiple sources for specific tissues or cells still has great challenges.
Disclosure of Invention
The invention aims to provide a double-layer exosome with a core-shell structure.
Yet another object of the present invention is to: provides a preparation method of the double-layer exosome with the core-shell structure.
The purpose of the invention is realized by the following scheme: a double-layer exosome with a nucleocapsid structure is characterized in that the exosome has a nucleocapsid structure derived from 2 cells.
The invention provides a preparation method of a double-layer exosome with a core-shell structure, which is characterized by comprising the following steps:
(1) preparation of chitosan/exosome nano-particle
1-5 g of chitosan is dissolved in 100 ml of 1% acetic acid, and the mixture is stirred for at least 1 hour until the chitosan is completely dissolved. Adding 1-10 g of exosome solution with the concentration of 200 micrograms/ml into the solution, adding 60 ml of surfactant aqueous solution with the mass ratio of 5%, stirring by using a high-speed stirrer to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain the chitosan/exosome nanoparticle.
(2) Preparation of double-layer exosomes
And co-incubating the chitosan/exosome nanoparticles and intestinal macrophages at a ratio of nanoparticles to macrophages of 1:10-1:50, culturing for 12-72 hours, and removing supernatant when the cell fusion degree reaches about 60% -95%. And centrifuging the supernatant at low temperature at low speed to remove dead cells, and centrifuging at low temperature at high speed again to obtain the macrophage exosome coated with the chitosan/exosome nanoparticles, wherein the exosome is a double-layer exosome with a core-shell structure.
The surfactant is one or a mixture of more of Triton X-100 (polyethylene glycol octyl phenyl ether), Tu-80 (polyoxyethylene sorbitan monooleate), Span-60 (sorbitan monostearate), 6501 (coconut oil fatty acid diethanolamide), AEO-9 (fatty alcohol polyoxyethylene ether) and Brij-35 (lauryl alcohol polyoxyethylene ether).
In the step 1, the stirring condition is that the rotating speed is 1500-30000 rpm, and the stirring time is 3-5 minutes.
In the step 2, the low-speed centrifugation condition at low temperature is 2-6 ℃, the centrifugation speed is 1000-2200 g, and the centrifugation time is 10-80 minutes.
In the step 2, the high-speed centrifugation condition at low temperature is 2-6 ℃, the centrifugation speed is 5000-.
The invention provides an exosome with multiple sources and a preparation method thereof, in particular to a double-layer exosome and a preparation method thereof.
The invention has the advantages that:
(1) can simultaneously realize exosomes with multiple sources, and increases the functions of exosomes.
(2) The preparation method has the advantages of simple preparation process, easy control of reaction, good stability and industrialization.
Detailed Description
The technical solution of the present invention is further described below by specific examples. The following examples are further illustrative of the present invention and do not limit the scope of the present invention.
Example 1
A double-layer exosome with a nucleocapsid structure, which has a nucleocapsid structure derived from 2 cells, is prepared by the following steps:
(1) preparation of chitosan/exosome nano-particle
Preparing 1 g of chitosan solution dissolved in 100 ml of 1% acetic acid, and stirring for 2 hours until the chitosan is completely dissolved; adding 5 g of exosome solution with the concentration of 200 micrograms/ml into chitosan solution, adding 60 ml of surfactant Tu-80 aqueous solution with the mass ratio of 5%, stirring by using a high-speed stirrer at the rotating speed of 10000 rpm for 3 minutes to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain chitosan/exosome nanoparticles;
(2) preparation of double-layer exosomes
Co-incubating the chitosan/exosome nanoparticles and intestinal macrophages according to the proportion of 1:10, culturing for 24 hours until the cell fusion degree reaches 60%, and taking supernatant; and centrifuging the supernatant at the low temperature of 4 ℃ at the low speed of 2000 g for 60 minutes to remove dead cells, and centrifuging at the low temperature of 4 ℃ at the high speed of 6000 g for 80 minutes to obtain the intestinal macrophage double-layer exosome coated with the chitosan/exosome nanoparticles.
Example 2
A double-layer exosome with a nucleocapsid structure, which has a nucleocapsid structure derived from 2 cells, is prepared by the following steps:
(1) preparation of chitosan/exosome nano-particle
Preparing a solution of 3 g of chitosan dissolved in 100 ml of 1% acetic acid, and stirring for 2 hours until the chitosan is completely dissolved; adding 5 g of exosome solution with the concentration of 200 micrograms/ml into chitosan solution, adding 60 ml of surfactant Brij-35 aqueous solution with the mass ratio of 5%, stirring by using a high-speed stirrer at the rotating speed of 15000 rpm for 3 minutes to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain chitosan/exosome nanoparticles;
(2) preparation of double-layer exosomes
Co-incubating the chitosan/exosome nanoparticles and intestinal macrophages according to the proportion of 1:20, culturing for 48 hours until the cell fusion degree reaches 90%, and taking supernatant; and centrifuging the supernatant at a low speed of 1500 g at a low temperature of 4 ℃ for 80 minutes to remove dead cells, and centrifuging at a low temperature of 4 ℃ at a high speed of 10000 g for 60 minutes to obtain the intestinal macrophage double-layer exosome coated with the chitosan/exosome nanoparticles.
Example 3
A double-layer exosome with a nucleocapsid structure, which has a nucleocapsid structure derived from 2 cells, is prepared by the following steps:
(1) preparation of chitosan/exosome nano-particle
Preparing a solution of 5 g of chitosan dissolved in 100 ml of 1% acetic acid, and stirring for 4 hours until the chitosan is completely dissolved; adding 10 g of exosome solution with the concentration of 200 micrograms/ml into chitosan solution, adding 60 ml of surfactant Triton X-100 aqueous solution with the mass ratio of 5%, stirring by using a high-speed stirrer at the rotating speed of 30000 rpm for 5 minutes to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain chitosan/exosome nanoparticles;
(2) preparation of double-layer exosomes
Co-incubating the chitosan/exosome nanoparticles and intestinal macrophages according to the proportion of 1:50, culturing for 72 hours until the cell fusion degree reaches 90%, and taking supernatant; centrifuging the supernatant at the low temperature of 4 ℃ at the speed of 1500 g for 80 minutes at a low speed to remove dead cells, and centrifuging at the low temperature of 4 ℃ at the speed of 12000 g for 60 minutes at a high speed to obtain the intestinal macrophage double-layer exosome coated with the chitosan/exosome nanoparticles.
Claims (9)
1. A double-layer exosome with a nucleocapsid structure is characterized in that the exosome has a nucleocapsid structure derived from 2 cells.
2. A method for preparing a double-layer exosome of core-shell structure according to claim 1, comprising the following steps:
(1) preparation of chitosan/exosome nano-particle
Preparing 1-5 g of chitosan, dissolving the chitosan in 100 ml of 1% acetic acid solution, stirring for at least 1 hour, adding 1-10 g of exosome solution with the concentration of 200 micrograms/ml into the solution when the chitosan is completely dissolved, then adding 60 ml of aqueous solution containing 5% of surfactant by mass, stirring by using a high-speed stirrer to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain chitosan/exosome nanoparticles;
(2) preparation of double-layer exosomes
Co-incubating the chitosan/exosome nanoparticles and intestinal macrophages at a ratio of nanoparticles to macrophages of 1:10-1:50, culturing for 12-72 hours until the cell fusion degree reaches 60% -95%, and removing supernatant; and centrifuging the supernatant at low temperature at low speed to remove dead cells, and centrifuging at low temperature at high speed again to obtain the macrophage exosome coated with the chitosan/exosome nanoparticles, wherein the exosome is a double-layer exosome with a core-shell structure.
3. The method for preparing a bilayer exosome with a core-shell structure according to claim 2, wherein the surfactant is one or a mixture of several of Triton X-100 (polyethylene glycol octyl phenyl ether), Tu-80 (polyoxyethylene sorbitan monooleate), Span-60 (sorbitan monostearate), 6501 (coconut oil fatty acid diethanolamide), AEO-9 (fatty alcohol polyoxyethylene ether) and Brij-35 (lauryl alcohol polyoxyethylene ether).
4. The method for preparing a double-layer exosome with a core-shell structure according to claim 2, wherein in the step (1), the stirring speed is 1500-30000 rpm, and the stirring time is 3-5 minutes.
5. The method for preparing a double-layer exosome with a core-shell structure according to claim 2, wherein in the step 2, the low-speed centrifugation condition at low temperature is 2-6 ℃, the centrifugation speed is 1000-2200 g, and the centrifugation time is 10-80 minutes.
6. The method for preparing a double-layer exosome with a core-shell structure according to claim 2, wherein in the step 2, the high-speed centrifugation condition at low temperature is 2-6 ℃, the centrifugation speed is 5000-.
7. The method for preparing a bilayer exosome of core-shell structure according to any one of claims 2 to 6, which is characterized by comprising the following steps:
(1) preparation of chitosan/exosome nano-particle
Preparing 1 g of chitosan solution dissolved in 100 ml of 1% acetic acid, and stirring for 2 hours until the chitosan is completely dissolved; adding 5 g of exosome solution with the concentration of 200 micrograms/ml into chitosan solution, adding 60 ml of surfactant Tu-80 aqueous solution with the mass ratio of 5%, stirring by using a high-speed stirrer at the rotating speed of 10000 rpm for 3 minutes to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain chitosan/exosome nanoparticles;
(2) preparation of double-layer exosomes
Co-incubating the chitosan/exosome nanoparticles and intestinal macrophages according to the proportion of 1:10, culturing for 24 hours until the cell fusion degree reaches 60%, and taking supernatant; and centrifuging the supernatant at the low temperature of 4 ℃ at the low speed of 2000 g for 60 minutes to remove dead cells, and centrifuging at the low temperature of 4 ℃ at the high speed of 6000 g for 80 minutes to obtain the intestinal macrophage double-layer exosome coated with the chitosan/exosome nanoparticles.
8. The method for preparing a bilayer exosome of core-shell structure according to any one of claims 2 to 6, which is characterized by comprising the following steps:
(1) preparation of chitosan/exosome nano-particle
Preparing a solution of 3 g of chitosan dissolved in 100 ml of 1% acetic acid, and stirring for 2 hours until the chitosan is completely dissolved; adding 5 g of exosome solution with the concentration of 200 micrograms/ml into chitosan solution, adding 60 ml of surfactant Brij-35 aqueous solution with the mass ratio of 5%, stirring by using a high-speed stirrer at the rotating speed of 15000 rpm for 3 minutes to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain chitosan/exosome nanoparticles;
(2) preparation of double-layer exosomes
Co-incubating the chitosan/exosome nanoparticles and intestinal macrophages according to the proportion of 1:20, culturing for 48 hours until the cell fusion degree reaches 90%, and taking supernatant; and centrifuging the supernatant at a low speed of 1500 g at a low temperature of 4 ℃ for 80 minutes to remove dead cells, and centrifuging at a low temperature of 4 ℃ at a high speed of 10000 g for 60 minutes to obtain the intestinal macrophage double-layer exosome coated with the chitosan/exosome nanoparticles.
9. The method for preparing a bilayer exosome of core-shell structure according to any one of claims 2 to 6, which is characterized by comprising the following steps:
(1) preparation of chitosan/exosome nano-particle
Preparing a solution of 5 g of chitosan dissolved in 100 ml of 1% acetic acid, and stirring for 4 hours until the chitosan is completely dissolved; adding 10 g of exosome solution with the concentration of 200 micrograms/ml into chitosan solution, adding 60 ml of surfactant Triton X-100 aqueous solution with the mass ratio of 5%, stirring by using a high-speed stirrer at the rotating speed of 30000 rpm for 5 minutes to form water-in-oil (w/o) emulsion of exosome, and freeze-drying to obtain chitosan/exosome nanoparticles;
(2) preparation of double-layer exosomes
Co-incubating the chitosan/exosome nanoparticles and intestinal macrophages according to the proportion of 1:50, culturing for 72 hours until the cell fusion degree reaches 90%, and taking supernatant; centrifuging the supernatant at the low temperature of 4 ℃ at the speed of 1500 g for 80 minutes at a low speed to remove dead cells, and centrifuging at the low temperature of 4 ℃ at the speed of 12000 g for 60 minutes at a high speed to obtain the intestinal macrophage double-layer exosome coated with the chitosan/exosome nanoparticles.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115364212A (en) * | 2022-08-13 | 2022-11-22 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of tumor cell exosome loaded gold nanoparticles, product and application thereof |
Citations (3)
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|---|---|---|---|---|
| CN107582567A (en) * | 2017-09-06 | 2018-01-16 | 李征宇 | A kind of excretion body targeted sustained release microsphere biological support and its production and use |
| US20210130782A1 (en) * | 2019-10-28 | 2021-05-06 | Augusta University Research Institute, Inc. | Engineered Exosomes to Detect and Deplete Pro-Tumorigenic Macrophages |
| CN112933113A (en) * | 2021-02-24 | 2021-06-11 | 江南大学附属医院 | Immune-enhanced exosome hydrogel compound and preparation method and application thereof |
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- 2021-12-17 CN CN202111552591.XA patent/CN114224868A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582567A (en) * | 2017-09-06 | 2018-01-16 | 李征宇 | A kind of excretion body targeted sustained release microsphere biological support and its production and use |
| US20210130782A1 (en) * | 2019-10-28 | 2021-05-06 | Augusta University Research Institute, Inc. | Engineered Exosomes to Detect and Deplete Pro-Tumorigenic Macrophages |
| CN112933113A (en) * | 2021-02-24 | 2021-06-11 | 江南大学附属医院 | Immune-enhanced exosome hydrogel compound and preparation method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115364212A (en) * | 2022-08-13 | 2022-11-22 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of tumor cell exosome loaded gold nanoparticles, product and application thereof |
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