CN114214253B - Method for improving yield of antibacterial lipopeptide fengycin of bacillus amyloliquefaciens - Google Patents
Method for improving yield of antibacterial lipopeptide fengycin of bacillus amyloliquefaciens Download PDFInfo
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Abstract
The invention discloses a method for improving the yield of antibacterial lipopeptide fengycin of bacillus amyloliquefaciens, which comprises the steps of inoculating bacillus amyloliquefaciens into a fermentation culture medium containing montmorillonite powder for fermentation culture, and improving the yield of the antibacterial lipopeptide fengycin. According to the invention, bacillus amyloliquefaciens is used as a strain, and montmorillonite powder with a certain concentration is added into a fermentation system to improve medium transmission of the fermentation system, so that the Fengycin yield can be remarkably improved. According to the invention, the powdery crude extract of the Fengycin is obtained through fermentation, concentration, extraction and freeze-drying, and the results show that the yield of the Fengycin can be obviously improved by montmorillonite powder with proper concentration through a weighing method and HPLC detection, and the maximum yield can reach 5.89g/L, and the yield of the Fengycin is improved by 2.65 times compared with the yield of a control group without montmorillonite powder. The invention can obviously improve the yield of fengycin and reduce the production cost.
Description
Technical Field
The invention belongs to the technical field of industrial microorganisms, and particularly relates to a method for improving yield of antibacterial lipopeptid fengycin of bacillus amyloliquefaciens.
Background
Along with popularization of the concept of green health and environmental protection sustainable development and enhancement of pathogen resistance caused by antibiotic abuse, antibiotic substitutes are becoming research hotspots for people. Fengycin is lipopeptide synthesized by bacillus amyloliquefaciens through a non-ribosome way and capable of strongly inhibiting filamentous fungi, is considered to be developed into a novel preservative and an antibacterial drug due to the advantages of good stability, high safety, difficulty in drug resistance generation and the like, is considered as one of future antibiotic substitutes, and is expected to make up for the current ubiquitous problem of antibiotic drug resistance. Recent researches also show that the cyclic amino acid and chain lipid structures of the Fengycin are similar to the chemical structures of self-induced peptides (AIPs) generated in pathogenic bacteria staphylococcus aureus quorum sensing systems, the Fengycin can be combined with AIPs on the cell surfaces of the pathogenic bacteria staphylococcus aureus quorum sensing systems through competitive inhibition, the population stability of the staphylococcus aureus is disturbed, the colonisation of the staphylococcus aureus in intestinal tracts is inhibited, and the research also shows that the Fengycin can inhibit the proliferation and the diffusion of tumor cells. In conclusion, the Fengycin has broad-spectrum antibacterial activity, and has wide application prospects in aspects of food preservation, agricultural application production, clinical medical treatment and the like. The maximum resistance that today prevents the application of Fengycin is due to its lower yield, but the low yield prevents its further development and use, so that increasing the Fengycin yield is a problem to be solved. Most researchers have reported to increase their yields by optimizing the medium composition or genetic engineering molecular manipulation, while there are few reports on the biocatalyst method to increase their yields.
The montmorillonite powder is the montmorillonite powder micro powder containing the dioctahedral, has a layered structure and non-uniform charge distribution, is developed into a probiotic coating preparation due to strong adsorptivity and fixing performance, can promote intestinal field planting of probiotics, has strong covering protection capability on alimentary canal mucous membrane, and has the functions of repairing and improving the defending function of mucous membrane barrier on attack factors. However, no related report exists on the interaction of montmorillonite powder in the metabolism process of microorganisms.
Disclosure of Invention
The invention aims to: aiming at the problems existing in the prior art, the invention provides a novel method for improving the yield of the antibacterial lipopeptide Fengycin of bacillus amyloliquefaciens. Compared with the conventional culture medium optimization method and the method for improving the lipopeptid Fengycin by genetically engineering strains, the method has the advantages of low cost, strong operability, high stability and the like, and has the potential of expanding the application of the Fengycin market.
The technical scheme is as follows: in order to achieve the above purpose, according to the method for improving the yield of the antibacterial lipopeptide fengycin of the bacillus amyloliquefaciens, the bacillus amyloliquefaciens is inoculated into a fermentation culture medium containing montmorillonite powder for fermentation culture, and the yield of the antibacterial lipopeptide fengycin is improved.
Preferably, the bacillus amyloliquefaciens is bacillus amyloliquefaciens CGMCC No.18719 or CGMCC No.18720.
Wherein the method comprises the following steps:
(1) Activating and culturing bacillus amyloliquefaciens to obtain a seed culture solution;
(2) Inoculating the seed culture solution obtained in the step (1) into a fermentation culture medium according to the inoculum size of 1% -5% by volume, then adding montmorillonite powder, and culturing at 30-37 ℃;
(3) Centrifuging the fermentation broth obtained in the step (2), and discarding a precipitate mixture of the degerming body and the montmorillonite powder to obtain a cell-free supernatant;
(4) Regulating the pH of the cell-free supernatant obtained in the step (3) to be subjected to acid precipitation overnight;
(5) Centrifuging the precipitate obtained in the step (4), collecting the precipitate, adding water to adjust to be neutral, freeze-drying, extracting with methanol, and performing rotary evaporation on the methanol extraction mixture to obtain a Fengycin crude extract freeze-dried powder;
(6) Dissolving and filtering the lipopeptide crude extract dry powder obtained in the step (5), and identifying by utilizing HPLC.
Wherein, the liquid activation culture medium for the activation culture in the step (1) comprises the following components: every 1L comprises 5-10g of beef extract, 5-20g of peptone, 5-10g of yeast extract, 5-10g of NaCl and 5-10g of glucose; bran 1-5g and pH 6.5-7.0.
Wherein, the activating culture condition in the step (1) is 30-37 ℃, 150-220 rmp, culturing for 8-16 h, and standby when the detection OD600 = 0.5-1, thus obtaining the seed liquid.
Wherein the fermentation medium of step (2) comprises per 1L: 1-5g of beef extract, 5-20g of sodium glutamate, 0.5-1g of KCl, 1-3g of NaCl and KH 2 PO 4 1-2g，MgSO 4 0.5-1g，MnSO 4 1-5mg，CuSO 4 0.05-0.16mg，FeSO 4 ·7H 2 O 0.05-0.19mg，ZnSO 4 0.05-0.5g, 10-50g of fructose and 10-30g of glucose.
Wherein, the montmorillonite powder in the step (2) is exposed to ultraviolet lamp irradiation and then added into the fermentation medium.
Wherein the addition amount of the montmorillonite powder in the step (2) is 0.01-1g per 100mL of fermentation medium.
Wherein the fermentation condition in the step (2) is that the fermentation is carried out at 29-37 ℃ for 50-74h.
According to the invention, by adding a large-surface-area solid matrix in the fermentation process of bacillus amyloliquefaciens, the effect on the yield of the bacillus amyloliquefaciens is found to be obvious, and the addition of montmorillonite powder possibly increases the concentration of thalli or adsorbs some signal substances in the fermentation process of strains, and the antibacterial effect is verified by showing an acid precipitation picture, detecting and comparing the effect of the addition of montmorillonite powder on the yield of the bacillus amyloliquefaciens through high performance liquid chromatography and then through a Fengycin antibacterial test.
The beneficial effects are that: compared with the prior art, the invention has the following advantages:
according to the invention, montmorillonite powder is added into a bacillus amyloliquefaciens fermentation system, so that the yield of a secondary metabolite Fengycin can be remarkably improved.
The invention has low operation cost, high repeatability and wide application range.
Drawings
FIG. 1 is a graph of the present invention showing a comparison of precipitation after acid precipitation (attempted comparison of acid precipitation after addition of 0.1g of montmorillonite powder, montmorillonite powder group (left), montmorillonite powder group (right)) without addition of montmorillonite powder group;
FIG. 2 is a HPLC chart of a Fengycin standard;
FIG. 3 is an HPLC diagram of a liquid fermentation product of Bacillus amyloliquefaciens (Fengycin HPLC detection of montmorillonite powder group added fermentation broth);
FIG. 4 is an HPLC chart of a liquid fermentation product of Bacillus amyloliquefaciens (Fengycin HPLC detection of the non-montmorillonite powder added fermentation group);
fig. 5, 6, 7, 8 and 9 show the primary mass spectra (mass to charge ratios 1450.2, 1464.3, 1478.4, 1492.1 and 1506.5) of the Fengycin crude extract.
Detailed Description
The invention is further described below with reference to the drawings and examples.
Materials, reagents, and the like used in the examples of the present invention are commercially available unless otherwise specified. The experimental methods for which specific conditions are not specified in the examples are generally conducted under conventional conditions or under conditions recommended by the manufacturer.
Wherein, the bacillus amyloliquefaciens CGMCC No.18719 and CGMCC No.18720 are provided by Huaiyin institute of technology, and the applicant has disclosed preservation in the prior patent CN 110951667A.
Montmorillonite powder was purchased from the biosciences of Huaian fibrate.
The components of the solid activation medium are: beef extract 5g, peptone 10g, yeast extract 5g, naCl5g, glucose 10g, bran 2g, agar powder 1.5g, distilled water to 1L, pH=7.0, sterilizing at 115 ℃ for 30min; the liquid activation medium does not contain agar powder.
Example 1
And streaking the preserved bacillus amyloliquefaciens CGMCC NO.18719 on a solid activation culture medium, picking out single bacterial colony and inoculating the single bacterial colony into a liquid activation culture medium. The activating culture condition is 35 ℃,180rmp, and culturing for 12 hours, and when the OD600 = 1.0 is detected, the seed liquid is prepared for standby.
Example 2
Fermentation of Bacillus amyloliquefaciens
(1) Weighing montmorillonite powder and ultraviolet sterilizing
Accurately weighing 0.05g, 0.1g, 0.5g and 1g of montmorillonite powder, and exposing the montmorillonite powder to an ultraviolet lamp for 60min;
(2) Preparation of fermentation medium and high-temperature sterilization
Accurately weighing 1g of beef extract and 14g,KCl 0.5g,NaCl 2.5g,KH g of sodium glutamate 2 PO 4 1g，MgSO 4 0.5g，MnSO 4 5mg，CuSO 4 0.16mg，FeSO 4 ·7H 2 0.15mg,ZnSO4 0.12g, 40g of fructose, 25g of glucose, distilled water to a volume of 1L, pH=7.0, and sterilizing at 115 ℃ for 30min;
in example 1, the seed solution was inoculated into a fermentation medium (100 mL) at an inoculum size of 5% by volume, 0.1g of montmorillonite powder was weighed accurately and added to the fermentation medium, 3 parallel culture flasks without montmorillonite powder were set for each group, and a blank was used as a control. After the completion of the addition, the flask was incubated at 33℃and 180rmp for 72 hours.
Example 3
Separation and content detection of Fengycin
(1) Preparation of cell-free supernatant
Separately filling the fermentation broth of example 2 into 500ml centrifuge cups, centrifuging at 4℃with a cryocentrifuge, and centrifuging at 5,000Xg for 20min; discarding the precipitate mixture of the degerming body and the montmorillonite powder to obtain cell-free supernatant;
(2) Preparation of Fengycin crude extract
The cell-free supernatant obtained in (1) was adjusted to pH 2.0 with 6mol/L HCl and left to stand in a refrigerator overnight. Centrifuging the system after acid precipitation again at 4deg.C for 20min at 5,000Xg, discarding supernatant, adding distilled water, stirring at low temperature to adjust pH to 7.0, lyophilizing to obtain powder, extracting with methanol, and making the methanol extract mixture into lipopeptide crude extract dry powder at 45deg.C in rotary vacuum evaporator, and storing in refrigerator;
the results of acid precipitation after adding 0.1g of montmorillonite powder are shown in fig. 1, and the research results show that the substances subjected to acid precipitation overnight are mainly lipopeptides substances, and the addition of montmorillonite powder significantly increases the content of lipopeptides substances in the fermentation broth (fig. 1, left).
(3) HPLC detection of Fengycin
The crude lipopeptide extract (0.1 g of montmorillonite powder was added) obtained in step (2) was dissolved in methanol to give a final concentration of 0.5mg/ml, and the resulting solution was filtered through a 0.22 μm organic filter membrane and then identified by HPLC. The chromatographic column is as follows: agilent c18 column, mobile phase a: acetonitrile (0.1% trifluoroacetic acid), mobile phase B: deionized water (containing 0.1% trifluoroacetic acid), flow rate: UV detection at 205nm at 0.6mL/min, elution procedure is shown in Table 1, and each sample was run in parallel 3 times.
Table 1HPLC gradient elution procedure
Through HPLC detection, the peak shape of the fermentation substance is consistent with the peak shape of a Fengycin standard substance (figure 2, zhou Zhen. Research on the gene expression regulation of Bacillus amyloliquefaciens Fengycin synthetase [ D ]: huai-yin institute of technology; 2020), wherein 1-6 are characteristic peaks of Fengycin, compared with figure 4 (a fermentation system without montmorillonite powder), the peak area change of figure 3 is obvious, the peak area change is consistent with the weighing result of acid precipitation and dry powder, and the maximum yield is up to 5.89g/L, which is improved by 2.65 times compared with the Fengycin without montmorillonite powder.
(4) Fengycin mass spectrometry verification
The molecular weight of the lipopeptides homologs in the crude extract was determined by time-of-flight mass spectrometry. Fengycin has many homologous compounds and the structures of the same molecular weight of the anemocins may be different. The mass spectrum ion peaks of each stage of the anemonin are further separated through ESI/CID, and the obtained mass spectrum ion peaks have certain regularity. The antimicrobial lipopeptides were identified as Fengycin and homologs by the Fengycin feature m/z results (mass to charge ratios 1450.2, 1464.3, 1478.4, 1492.1, 1506.5) (FIGS. 5-9).
Meanwhile, 0.05g, 0.5g and 1g of montmorillonite powder are respectively subjected to fermentation experiments according to the same method, so that the Fengycin yield can be effectively improved, but the effect is not obvious like that of 0.1g of montmorillonite powder, which indicates that the montmorillonite powder cannot be excessively or excessively added.
Example 4
In example 1, the seed solution was inoculated into a fermentation medium (100 mL) at an inoculum size of 1% by volume, 0.1g of montmorillonite powder was weighed accurately and added to the fermentation medium, 3 parallel culture flasks without montmorillonite powder were set for each group, and a blank was used as a control. After the completion of the addition, the flask was incubated at 29℃and 180rmp for 74 hours.
Example 5
In example 1, the seed solution was inoculated into a fermentation medium (100 mL) at an inoculum size of 3% by volume, 0.1g of montmorillonite powder was weighed accurately and added to the fermentation medium, 3 parallel culture flasks without montmorillonite powder were set for each group, and a blank was used as a control. After the completion of the addition, the flask was incubated at 37℃and 180rmp for 50 hours.
Claims (8)
1. A method for improving the yield of antibacterial lipopeptide fengycin of bacillus amyloliquefaciens is characterized in that bacillus amyloliquefaciens is inoculated into a fermentation medium containing montmorillonite powder for fermentation culture, and the yield of the antibacterial lipopeptide fengycin is improved; the bacillus amyloliquefaciens is bacillus amyloliquefaciens CGMCC No.18719; the addition amount of the montmorillonite powder is 0.1 and g per 100mL of fermentation medium.
2. The method for improving yield of antibacterial lipopeptide fengycin of bacillus amyloliquefaciens according to claim 1, wherein the method comprises the following steps:
(1) Activating and culturing bacillus amyloliquefaciens to obtain a seed culture solution;
(2) Inoculating the seed culture solution obtained in the step (1) into a fermentation culture medium according to the inoculum size of 1% -5% by volume, then adding montmorillonite powder, and culturing at 30-37 ℃;
(3) Centrifuging the fermentation broth obtained in the step (2), and discarding a precipitate mixture of the degerming body and the montmorillonite powder to obtain a cell-free supernatant;
(4) Regulating the pH of the cell-free supernatant obtained in the step (3) to be subjected to acid precipitation overnight;
(5) Centrifuging the precipitate obtained in the step (4), collecting the precipitate, adding water to adjust to be neutral, freeze-drying, extracting with methanol, and performing rotary evaporation on the methanol extraction mixture to obtain the Fengycin crude extract dry powder.
3. The method for improving the yield of the antibacterial lipopeptid fengycin of the bacillus amyloliquefaciens according to claim 2, wherein the liquid component of the activation culture in the step (1) comprises 5-10g of beef extract, 5-20g of peptone, 5-10g of yeast extract, 5-10g of NaCl and 5-10g of glucose per 1L; bran 1-5g and pH 6.5-7.0.
4. The method for improving the yield of the antibacterial lipopeptid fengycin of the bacillus amyloliquefaciens according to claim 2, wherein the activating culture condition in the step (1) is 30-37 ℃, 150-220 rmp, and culturing for 8-16 h, and the seed liquid is prepared after the detection of OD < 600 > = 0.5-1 for later use.
5. The method for improving yield of antibacterial lipopeptide fengycin of bacillus amyloliquefaciens according to claim 2, wherein the method comprises the following steps ofWherein the fermentation medium of step (2) comprises per 1L: 1-5g of beef extract, 5-20g of sodium glutamate, 0.5-1g of KCl, 1-3g of NaCl and KH 2 PO 4 1-2g，MgSO 4 0.5-1g，MnSO 4 1-5mg，CuSO 4 0.05-0.16 mg，FeSO 4 ·7H 2 O 0.05-0.19mg，ZnSO 4 0.05-0.5g, 10-50g g g of fructose and 10-30g of glucose.
6. The method for improving yield of antibacterial lipopeptid fengycin of bacillus amyloliquefaciens according to claim 2, wherein the montmorillonite powder in the step (2) is exposed to ultraviolet lamp irradiation and then added into a fermentation medium.
7. The method for improving yield of antibacterial lipopeptid fengycin of bacillus amyloliquefaciens according to claim 2, wherein the fermentation condition in the step (2) is that the fermentation is carried out at 29-37 ℃ for 50-74h.
8. An application of montmorillonite powder in improving the yield of antibacterial lipopeptid fengycin of bacillus amyloliquefaciens, wherein the bacillus amyloliquefaciens is bacillus amyloliquefaciens CGMCC No.18719; the addition amount of the montmorillonite powder is 0.1 and g per 100mL of fermentation medium.
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