CN114208811A - A seminal fluid refrigerating plant for hu sheep breeding - Google Patents
A seminal fluid refrigerating plant for hu sheep breeding Download PDFInfo
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- CN114208811A CN114208811A CN202111553687.8A CN202111553687A CN114208811A CN 114208811 A CN114208811 A CN 114208811A CN 202111553687 A CN202111553687 A CN 202111553687A CN 114208811 A CN114208811 A CN 114208811A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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Abstract
The invention discloses a semen freezing device for breeding Hu sheep, which comprises a shell, wherein a base is arranged at the bottom of the inner side of the shell, a rotating shaft is arranged on the base, a freezing box is arranged on the upper side of the rotating shaft, a semen tube is placed in the freezing box, a rotating plate is arranged on the base, a guide rod is arranged in the shell, the lower end of the guide rod is in contact with one end of the rotating plate, a moving plate is arranged on one side of the shell, an ejector rod is arranged on the moving plate and is in contact with the other end of the rotating plate, and the upper end of the ejector rod can be in contact with the bottom of the freezing box. In the process of taking semen, the energy exchange between the inner side of the freezing box and the outside is greatly reduced, so that the inner side of the freezing box is in a low-temperature state, and favorable conditions are provided for semen storage.
Description
Technical Field
The invention belongs to the technical field of semen freezing equipment, and particularly relates to a semen freezing device for breeding Hu sheep.
Background
With the development of biotechnology, artificial insemination and embryo transfer techniques are indispensable important links in breeding Hu sheep. In order to improve the population scale and genetic quality of livestock animals or the meat quality of edible livestock animals, artificial insemination and breeding are needed for the livestock animals, but the semen of the male animals prepared in advance needs to be frozen in the transferring process, otherwise, the semen quality (such as sperm motility) of the male animals is seriously damaged, and the purpose of artificial insemination and breeding cannot be achieved.
At present, for giving the animal artificial insemination breeding for the animal husbandry that is located the remote pastoral area for the remote pastoral area, often transport male animal semen to the remote pastoral area with the help of various large-scale motor vehicles, in the transfer process, need carry out cryopreservation to semen with the help of semen refrigerating plant, reach the pastoral area after, need take out semen and carry out the work of breeding on next step, in order to keep the activity of semen at the in-process of breeding, always need to take out a liquid pipe of semen always, continue to take out next liquid pipe after finishing using, consequently need frequently to open the freezer and take the liquid pipe of semen, based on the structure of current freezer, frequent opening the freezer has increased the energy exchange between freezer and the external world, cause freezer energy loss very fast, be unfavorable for the preservation of semen.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a semen freezing device for breeding Hu sheep, which greatly reduces the energy exchange between the inner side of a freezing box and the outside in the process of taking semen, so that the upper time of the inner side of the freezing box is in a low-temperature state, and favorable conditions are provided for semen storage.
The invention provides the following technical scheme:
the utility model provides a seminal fluid refrigerating plant for hu sheep breeding, includes the casing, casing inside bottom is provided with the base, be provided with the pivot on the base, the pivot upside is provided with the freezer, the seminal fluid pipe has been placed in the freezer, be provided with the rotor plate on the base, be provided with the guide arm in the casing, the lower extreme of guide arm and the one end contact of rotor plate, one side of casing is provided with the movable plate, be provided with the ejector pin on the movable plate, the other end contact of ejector pin and rotor plate, the upper end of ejector pin can contact with the bottom of freezer.
Preferably, the bottom of freezer is provided with the ejecting hole, be provided with the support in the freezer, the support is provided with the fixed orifices, the fixed orifices is used for placing the semen pipe, the fixed orifices sets up with ejecting hole is coaxial.
Preferably, the limiting block is arranged on the inner wall of the shell, a through hole is formed in the limiting block, the guide rod is located in the through hole, and the guide rod can move in the through hole.
Preferably, the rotating plate is connected with the upright post through a rotating shaft, the upright post is arranged on the upper side of the base, a first sliding groove and a second sliding groove are formed in the rotating plate, a through hole is formed in the middle of the rotating plate, and the rotating shaft penetrates through the through hole.
Preferably, the surface of the freezing box is provided with a worm gear ring, the shell is provided with a worm, and the worm is meshed with the worm gear ring.
Preferably, a fixed block is arranged on the inner wall of the shell, the worm penetrates through the fixed block, and the worm can rotate in the fixed block.
Preferably, the upper surface of the shell is provided with an end cover, the end cover is provided with a groove, a plug body is arranged in the groove, the bottom of the groove is provided with an outlet, and the end cover is provided with a pressing block.
Preferably, one side of the rotating plate is provided with a spring, one end of the spring is connected with the rotating plate, and the other end of the spring is connected with the bottom of the shell.
Preferably, the movable plate includes square board and threaded rod, square board is connected with the threaded rod, be provided with the regulating part on the casing, the regulating part can rotate, the regulating part is connected with the threaded rod, be provided with the second stopper on the inner wall of casing, square board runs through the second stopper.
Preferably, the surface of pivot is provided with the locating hole, be provided with the constant head tank in the base, be provided with the locating lever in the constant head tank, be provided with the baffle on the locating lever, be provided with the second spring between the inner wall of baffle and constant head tank, the one end and the baffle of second spring are connected, and the other end is connected with the inner wall of constant head tank.
Preferably, an elastic bag is arranged on the upper side of the ejection hole, and a top plate is arranged at the top of the elastic bag.
Preferably, in order to increase the stress of the rotary freezer and facilitate the movement of the positioning rod to compress the second spring when the rotary freezer is rotated, the positioning hole is in a local spherical structure, the end part of the positioning rod is in a spherical structure, and the spherical radius of the positioning rod is R1The spherical radius of the positioning hole is R2The depth of the positioning hole is h, and the diameter of the rotating shaft is R3,R1、R2、R3H satisfies the following relationship:
2ΠR3h/NR2＝α·R1；
wherein N is the number of the positioning holes, alpha is a correlation coefficient, and the value range is 15-31.
Preferably, the use method of the semen freezing device for breeding the Hu sheep comprises the following steps:
s1, pressing the pressing block, wherein the pressing block acts on the guide rod, the guide rod further acts on the rotating plate, and the ejector rod rises to eject the semen tube;
s2, resetting the device, releasing the pressing block, resetting the guide rod under the action of the spring, and resetting the ejector rod under the action of gravity;
s3, rotating the freezing box, when the next semen pipe needs to be taken out, rotating the worm, enabling the worm to act on the freezing box, rotating the freezing box to enable the semen pipe to be opposite to the outlet, and then repeating the step S1;
and S4, after the taking is finished, covering a plug to reduce the heat exchange between the inner side of the device and the outside.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the sperm freezing device for Hu sheep breeding, the guide rod is arranged, the pressing block acts on the guide rod, the guide rod further acts on the rotating plate, the ejector rod rises to eject the sperm tube, the sperm tube taking process is more convenient and faster, an operator can be prevented from directly stretching into the freezing box to take the sperm tube, and the situation that the operator is frostbitten is avoided.
(2) According to the semen freezing device for Hu sheep breeding, the locating holes are formed, so that the freezing box can be accurately located when rotated, the semen pipe is accurately aligned to the outlet, the ejection of the semen pipe by the ejector rod is facilitated, and the use process of the device is more flexible.
(3) According to the semen freezing device for Hu sheep breeding, the worm gear ring and the worm are arranged and have a self-locking function, so that the freezing box can be kept in a static state in the process of taking the semen pipe, and the stability of the device is improved.
(4) The invention relates to a semen freezing device for Hu sheep breeding, which is characterized in that the spherical radius R of a positioning rod is limited and pressed1Spherical radius R of positioning hole2And the depth h of the positioning hole increases the stress of the rotary freezer, so that the device is more flexible in use and easy to position the freezer.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic view of the overall structure of the present invention.
Figure 2 is a schematic view of the freezer of the present invention.
Fig. 3 is a schematic view of the connection between the base and the rotating shaft of the present invention.
Fig. 4 is a schematic view of the moving plate of the present invention.
Fig. 5 is a schematic view of the rotating plate of the present invention.
Fig. 6 is a schematic view of the end cap of the present invention.
Figure 7 is a schematic view of the worm of the present invention in connection with a freezer.
FIG. 8 is a schematic view of a second stopper of the present invention.
Fig. 9 is a schematic view of an array of ejection holes and an array of holder fixing holes of the present invention.
In the figure: 1. a housing; 2. a limiting block; 3. a guide bar; 4. a rotating plate; 5. an end cap; 6. a plug body; 7. a freezer; 8. a fixed block; 9. a worm; 10. a second limiting block; 11. an adjustment member; 12. moving the plate; 13. a top rod; 14. a rotating plate; 15. a base; 16. a rotating shaft; 17. a spring; 18. a support; 19. positioning holes; 20. positioning a rod; 21. a baffle plate; 22. a second spring; 23. positioning a groove; 24. a first chute; 25. a through hole; 26. a second chute; 27. a worm gear ring; 28. an elastic bag; 29. a top plate; 30. an outlet; 31. a square plate; 32. a threaded rod; 33. and ejecting the hole.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described in detail and completely with reference to the accompanying drawings. It is to be understood that the described embodiments are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
The first embodiment is as follows:
as shown in fig. 1-8, a semen freezing device for breeding hu sheep comprises a housing 1, wherein a base 15 is arranged at the bottom inside the housing 1, a rotating shaft 16 is arranged on the base 15, the rotating shaft 16 can rotate on the base 15, a freezing chamber 7 is arranged on the upper side of the rotating shaft 16, a semen pipe is arranged in the freezing chamber 7, hu sheep semen is stored in the semen pipe, liquid nitrogen is contained in the freezing chamber 7 and used for freezing semen, a rotating plate 14 is arranged on the base 15, a guide rod 3 is arranged in the housing 1, the lower end of the guide rod 3 is in contact with one end of the rotating plate 14, a moving plate 12 is arranged on one side of the housing 1, a push rod 13 is arranged on the moving plate 12, the push rod 13 is in contact with the other end of the rotating plate 14, the upper end of the push rod 13 can be in contact with the bottom of the freezing chamber 7, the ejector rod 13 is used for ejecting the semen tube, so that the semen tube can be taken conveniently.
The bottom of freezer chest 7 is provided with ejection hole 33, ejection hole 33 is the circular array setting, be provided with support 18 in the freezer chest 7, support 18 is provided with the fixed orifices, the fixed orifices is used for placing the seminal fluid pipe, the fixed orifices sets up with ejection hole 33 is coaxial, the fixed orifices corresponds the setting with ejection hole, and every seminal fluid pipe is located ejection hole 33 directly over promptly. The upper surface of the shell 1 is provided with an end cover 5, the end cover 5 is provided with a groove, a plug body 6 is arranged in the groove, the bottom of the groove is provided with an outlet 30, the outlet 30 is located right above the guide rod 3, the end cover 5 is provided with a pressing block 4, and the pressing block 4 is in contact with the upper end of the guide rod 3.
Spacing piece 2 is gone up to the inner wall of casing 1, be provided with the through-hole on the spacing piece 2, guide arm 3 is located the through-hole, guide arm 3 can remove in the through-hole. The rotating plate 14 is connected with an upright post through a rotating shaft, the upright post is arranged on the upper side of the base 15, a first sliding groove 24 and a second sliding groove 26 are arranged on the rotating plate 14, a through hole 25 is arranged in the middle of the rotating plate 14, and the rotating shaft 16 penetrates through the through hole 25. One side of the rotating plate 14 is provided with a spring 17, one end of the spring 17 is connected with the rotating plate 14, and the other end is connected with the bottom of the shell 1.
The surface of the freezing box 7 is provided with a worm gear ring 27, the shell 1 is provided with a worm 9, and the worm 9 is meshed with the worm gear ring 27. The inner wall of the shell 1 is provided with a fixing block 8, the worm 9 penetrates through the fixing block 8, and the worm 9 can rotate in the fixing block 8. Through rotating worm 9, can drive freezing case 7 and rotate, the station of transform essence pipe is convenient for take out the essence pipe in proper order.
The movable plate 12 comprises a square plate 31 and a threaded rod 32, the square plate 31 is connected with the threaded rod 32, an adjusting part 11 is arranged on the shell 1, the adjusting part 11 can rotate, the adjusting part 11 is connected with the threaded rod 32, a second limiting block 10 is arranged on the inner wall of the shell 1, the square plate 31 penetrates through the second limiting block 10, the adjusting part 11 rotates, the position of the movable plate 12 can be changed, the position of the ejector rod 13 is further changed, and the ejector rod 13 is located at the lower side of different ejector holes 33.
The surface of the rotating shaft 16 is provided with a positioning hole 19, a positioning groove 23 is arranged in the base 15, a positioning rod 20 is arranged in the positioning groove 23, a baffle 21 is arranged on the positioning rod 20, a second spring 22 is arranged between the baffle 21 and the inner wall of the positioning groove 23, one end of the second spring 22 is connected with the baffle 21, and the other end of the second spring is connected with the inner wall of the positioning groove 23. Adjacent included angle between the positioning holes 19 is equal to the included angle between the adjacent ejecting holes 33, the arrangement of the positioning holes 19 is convenient for positioning the freezing box, so that the ejecting holes 33 are positioned right above the ejector rods 13, and the semen tube is convenient to take out. The elastic bag 28 is provided on the upper side of the ejection hole 33, the top plate 29 is provided on the top of the elastic bag 28, and when the plunger 13 moves into the ejection hole 33, the plunger 13 is positioned inside the elastic bag 28, and the elastic bag 28 prevents the liquid nitrogen from leaking.
Example two:
the difference from the first embodiment is that, in order to increase the force applied to the rotary freezer 7 and facilitate the movement of the positioning rod 20 to compress the second spring 22 when the rotary freezer 7 is rotated, the positioning hole 19 is a partial sphere structure, the end of the positioning rod 20 is a spherical structure, and the spherical radius of the positioning rod 20 is R1The spherical radius of the positioning hole 9 is R2The depth of the positioning hole is h, and the diameter of the rotating shaft is R3,R1、R2、R3H satisfies the following relationship: 2 n R3h/NR2＝α·R1(ii) a Wherein N is the number of the positioning holes, alpha is a correlation coefficient, and the value range is 15-31. When the above relation is satisfied, the freezing chamber 7 is rotated, and after the positioning rod 20 is brought into contact with the positioning hole 19, the freezing chamber is rotatedThe shaft 16 is in a fixed state, and at this time, the force for rotating the worm is increased, and the feeling is relatively obvious, and the rotation of the freezer compartment 7 is stopped, and the lift pin 13 faces the lift pin hole 33. When alpha is less than 15, the terminal surface of locating lever 20 can not contact with locating hole 19 well, and fixed effect is relatively poor, and the stress state change of worm is less, and operating personnel's atress is relatively poor, and when alpha is greater than 31, the locating lever is great to the fixed power of counter shaft, when rotating the worm once more, needs great power to drive the freezer and rotates, uses to experience the sensation relatively poor.
EXAMPLE III
With reference to the second embodiment, when the α is 10, 15, 25, 30, 40, the device is tested to experience the change of the worm force at the moment when the rotating shaft is locked, when the α is 15, the positioning rod 20 is in contact with the positioning hole 19 with a slight noise, the operator can feel the slight change of the acting force when the worm rotates, when the α is 20, the positioning rod 20 is in contact with the positioning hole 19 with an obvious noise, the operator can feel the obvious change of the acting force when the worm rotates, when the α is 30, the positioning rod 20 is in contact with the positioning hole 19 with an obvious noise, the operator can feel the larger change of the acting force when the worm rotates, when the α is 40, the positioning rod 20 is in contact with the positioning hole 19 with an obvious noise, the operator can feel the larger change of the acting force when the worm rotates, when the worm rotates again, the freezer can rotate with a larger force, a large force is required to move the positioning rod 20 to compress the second spring 22.
Example four
The use method of the semen freezing device for Hu sheep breeding comprises the following steps:
s1, pressing the pressing block, wherein the pressing block acts on the guide rod, the guide rod further acts on the rotating plate, and the ejector rod rises to eject the semen tube;
s2, resetting the device, releasing the pressing block, resetting the guide rod under the action of the spring, and resetting the ejector rod under the action of gravity;
s3, rotating the freezing box, when the next semen pipe needs to be taken out, rotating the worm, enabling the worm to act on the freezing box, rotating the freezing box to enable the semen pipe to be opposite to the outlet, and then repeating the step S1;
and S4, after the taking is finished, covering a plug to reduce the heat exchange between the inner side of the device and the outside.
The device obtained by the technical scheme is a semen freezing device for breeding Hu sheep, in the using process, a semen pipe is placed into a freezing box, liquid nitrogen refrigerating liquid is added into the freezing box to freeze the semen pipe, when the semen pipe needs to be taken out, a plug body 6 is taken out, a rotating plate 4 is pressed, a mandril 13 ejects the semen pipe out to take out the corresponding semen pipe, when the semen pipe needs to be continuously taken out, a worm 9 is rotated to enable the freezing box to rotate, when a positioning rod 20 acts on the positioning hole 19, the force of the rotating worm 9 is instantly increased, at the moment, the rotating worm 9 is stopped, the rotating plate 4 is pressed to take out the semen pipe, the operation is circularly carried out, when the freezing box is rotated, the positioning rod 20 is in a spherical structure due to the fact that the positioning hole 19 is in the spherical structure, the positioning rod 20 can be separated from the positioning hole 19 by rotating the freezing box, a baffle 21 is further enabled to compress a second spring 22, after the semen pipe that is in the support outer lane takes and finishes, rotation regulation piece 11, change the position of movable plate 12, further change the position of ejector pin 13, make ejector pin 13 be in the downside of inner circle ejection hole 33, take out the semen pipe of inner circle one by one, direct contact in hand and the freezer has been avoided when taking out the semen pipe, the condition of frostbite hand has been avoided appearing, simultaneously when taking the semen pipe, the energy exchange between freezer and the external world has been reduced, energy loss has been avoided, the life of refrigerating fluid has been prolonged.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and it will be apparent to those skilled in the art that various modifications and variations can be made in the present invention; any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The utility model provides a seminal fluid refrigerating plant for hu sheep breeding, a serial communication port, includes casing (1), casing (1) inboard bottom is provided with base (15), be provided with pivot (16) on base (15), pivot (16) upside is provided with freezer (7), the seminal fluid pipe has been placed in freezer (7), be provided with rotor plate (14) on base (15), be provided with guide arm (3) in casing (1), the lower extreme of guide arm (3) and the one end contact of rotor plate (14), one side of casing (1) is provided with movable plate (12), be provided with ejector pin (13) on movable plate (12), ejector pin (13) and the other end contact of rotor plate (14), the upper end of ejector pin (13) can contact with the bottom of freezer (7).
2. The semen freezing device for Hu sheep breeding is characterized in that the bottom of the freezing box (7) is provided with an ejection hole (33), a bracket (18) is arranged in the freezing box (7), the bracket (18) is provided with a fixing hole, the fixing hole is used for placing a semen pipe, and the fixing hole is coaxial with the ejection hole (33).
3. The semen freezing device for Hu sheep breeding is characterized in that a limiting block (2) is arranged on the inner wall of the shell (1), a through hole is formed in the limiting block (2), the guide rod (3) is located in the through hole, and the guide rod (3) can move in the through hole.
4. The semen freezing device for Hu sheep breeding is characterized in that the rotating plate (14) is connected with an upright post through a rotating shaft, the upright post is arranged on the upper side of the base (15), a first sliding groove (24) and a second sliding groove (26) are arranged on the rotating plate (14), a through hole (25) is arranged in the middle position of the rotating plate (14), and the rotating shaft (16) penetrates through the through hole (25).
5. The semen freezing device for Hu sheep breeding is characterized in that the surface of the freezing box (7) is provided with a worm gear ring (27), the shell (1) is provided with a worm (9), and the worm (9) is meshed with the worm gear ring (27).
6. A semen freezing device for Hu sheep breeding according to claim 5, characterized in that the inner wall of the casing (1) is provided with a fixing block (8), the worm (9) penetrates the fixing block (8), and the worm (9) can rotate in the fixing block (8).
7. The semen freezing device for Hu sheep breeding is characterized in that an end cover (5) is arranged on the upper surface of the shell (1), a groove is arranged on the end cover (5), an outlet (30) is arranged at the bottom of the groove, and a pressing block (4) is arranged on the end cover (5).
8. The semen freezing device for Hu sheep breeding is characterized in that one side of the rotating plate (14) is provided with a spring (17), one end of the spring (17) is connected with the rotating plate (14), and the other end is connected with the bottom of the shell (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111553687.8A CN114208811A (en) | 2021-12-17 | 2021-12-17 | A seminal fluid refrigerating plant for hu sheep breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111553687.8A CN114208811A (en) | 2021-12-17 | 2021-12-17 | A seminal fluid refrigerating plant for hu sheep breeding |
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| Publication Number | Publication Date |
|---|---|
| CN114208811A true CN114208811A (en) | 2022-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111553687.8A Pending CN114208811A (en) | 2021-12-17 | 2021-12-17 | A seminal fluid refrigerating plant for hu sheep breeding |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180064383A1 (en) * | 2016-09-06 | 2018-03-08 | Shih-Hua HUANG | Multi-functional specimen collector |
| CN109874781A (en) * | 2019-03-12 | 2019-06-14 | 张敏 | A kind of reproductive medicine sperm storage device |
| CN210235720U (en) * | 2019-06-17 | 2020-04-03 | 中国人民解放军第四军医大学 | Procreation medical science is with low temperature preservation device |
-
2021
- 2021-12-17 CN CN202111553687.8A patent/CN114208811A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180064383A1 (en) * | 2016-09-06 | 2018-03-08 | Shih-Hua HUANG | Multi-functional specimen collector |
| CN109874781A (en) * | 2019-03-12 | 2019-06-14 | 张敏 | A kind of reproductive medicine sperm storage device |
| CN210235720U (en) * | 2019-06-17 | 2020-04-03 | 中国人民解放军第四军医大学 | Procreation medical science is with low temperature preservation device |
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