CN114203324A - Method for removing metal ions from biological agent and preparation method of biological agent - Google Patents
Method for removing metal ions from biological agent and preparation method of biological agent Download PDFInfo
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- CN114203324A CN114203324A CN202111341958.3A CN202111341958A CN114203324A CN 114203324 A CN114203324 A CN 114203324A CN 202111341958 A CN202111341958 A CN 202111341958A CN 114203324 A CN114203324 A CN 114203324A
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 229910021645 metal ion Inorganic materials 0.000 title claims abstract description 28
- 239000003124 biologic agent Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title abstract description 5
- 241000192091 Deinococcus radiodurans Species 0.000 claims abstract description 60
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 11
- 230000004071 biological effect Effects 0.000 claims abstract description 4
- -1 aluminum ions Chemical class 0.000 claims description 36
- 229910052782 aluminium Inorganic materials 0.000 claims description 20
- 229910001456 vanadium ion Inorganic materials 0.000 claims description 18
- 229910001449 indium ion Inorganic materials 0.000 claims description 17
- 229910052733 gallium Inorganic materials 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 3
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 239000013067 intermediate product Substances 0.000 claims description 2
- 230000005855 radiation Effects 0.000 abstract description 4
- 239000002351 wastewater Substances 0.000 description 68
- 241000588724 Escherichia coli Species 0.000 description 24
- 230000000694 effects Effects 0.000 description 12
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 238000004090 dissolution Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 229920006395 saturated elastomer Polymers 0.000 description 4
- 239000002354 radioactive wastewater Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- CKHJYUSOUQDYEN-UHFFFAOYSA-N gallium(3+) Chemical compound [Ga+3] CKHJYUSOUQDYEN-UHFFFAOYSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- 229910000616 Ferromanganese Inorganic materials 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 231100000569 acute exposure Toxicity 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000007494 tgy medium Substances 0.000 description 1
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- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21F—PROTECTION AGAINST X-RADIATION, GAMMA RADIATION, CORPUSCULAR RADIATION OR PARTICLE BOMBARDMENT; TREATING RADIOACTIVELY CONTAMINATED MATERIAL; DECONTAMINATION ARRANGEMENTS THEREFOR
- G21F9/00—Treating radioactively contaminated material; Decontamination arrangements therefor
- G21F9/04—Treating liquids
- G21F9/06—Processing
- G21F9/18—Processing by biological processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21F—PROTECTION AGAINST X-RADIATION, GAMMA RADIATION, CORPUSCULAR RADIATION OR PARTICLE BOMBARDMENT; TREATING RADIOACTIVELY CONTAMINATED MATERIAL; DECONTAMINATION ARRANGEMENTS THEREFOR
- G21F9/00—Treating radioactively contaminated material; Decontamination arrangements therefor
- G21F9/04—Treating liquids
- G21F9/20—Disposal of liquid waste
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/006—Radioactive compounds
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The disclosure belongs to the technical field of nuclear power, and particularly relates to a method for removing metal ions by using a biological agent and a preparation method of the biological agent. The method of the present disclosure comprises: continuously adding dried powder of deinococcus radiodurans with biological activity into liquid to be treated containing metal ions until the concentration of deinococcus radiodurans in the liquid to be treated reaches a preset concentration, wherein the preset concentration is between 0.8 and 1.6 grams per liter; and incubating the liquid to be treated containing deinococcus radiodurans with preset concentration so as to reduce the concentration of metal ions in the liquid to be treated. The method for removing the metal ions by the biological agent disclosed by the invention can be used for efficiently removing the metal ions in the liquid to be treated in a higher-dose radiation environment.
Description
Technical Field
The invention belongs to the technical field of nuclear power, and particularly relates to a method for removing metal ions by using a biological agent and a preparation method of the biological agent.
Background
Generally, the radioactive wastewater of a nuclear power plant contains a large amount of metal ions, and once the metal ions in the radioactive wastewater flow into the external environment, the metal ions seriously threaten the health and life of organisms in the external environment and seriously damage the natural ecosystem of the external environment. Therefore, what is needed is an efficient method for removing metal ions from radioactive wastewater.
Disclosure of Invention
In order to overcome the problems in the related art, a method for removing metal ions from a biological agent and a preparation method of the biological agent are provided.
According to an aspect of an embodiment of the present disclosure, there is provided a method of removing metal ions from a biological agent, the method including:
continuously adding dried powder of deinococcus radiodurans with biological activity into liquid to be treated containing metal ions until the concentration of deinococcus radiodurans in the liquid to be treated reaches a preset concentration, wherein the preset concentration is between 0.8 and 1.6 grams per liter;
and incubating the liquid to be treated containing deinococcus radiodurans with preset concentration so as to reduce the concentration of metal ions in the liquid to be treated.
In one possible implementation, the method further includes:
before the dry bacterial powder is added into the liquid to be treated, the hydrogen ion concentration index of the liquid to be treated is between 2.5 and 5.5.
In one possible implementation, the incubation treatment of the liquid to be treated containing a predetermined concentration of deinococcus radiodurans includes:
arranging a liquid to be treated containing deinococcus radiodurans with preset concentration on a constant-temperature shaking table;
and enabling the constant-temperature shaking table to continuously rotate at a preset rotating speed for a preset time.
In a possible implementation manner, the temperature of the constant temperature shaking table is between 16 and 30 ℃.
In one possible implementation, the preset rotation speed is between 100 and 200 revolutions per minute.
In one possible implementation, the preset time period is between 2-5 hours.
In one possible implementation manner, the metal ions contained in the liquid to be treated include any one or more of aluminum ions, gallium ions, vanadium ions, or indium ions.
According to another aspect of the embodiments of the present disclosure, there is provided a method of preparing a biological agent, the method including:
inoculating deinococcus radiodurans to a culture medium, culturing on a constant-temperature shaking table, collecting the culture medium by using a centrifugal machine under the condition that the cell density of deinococcus radiodurans in the culture medium is between 1.0 and 1.5, and freeze-drying an intermediate product obtained by centrifugal treatment in a freeze-drying machine to obtain the dry bacterium powder.
The beneficial effect of this disclosure lies in: the method for removing the metal ions by the biological agent disclosed by the invention can be used for efficiently removing the metal ions in the liquid to be treated in a higher-dose radiation environment.
Drawings
FIG. 1 is a flow chart illustrating a method of biological agent removal of metal ions according to an exemplary embodiment.
FIG. 2 is a graph showing the effect of treating vanadium ions in wastewater using deinococcus radiodurans in an application example.
FIG. 3 is a schematic diagram showing the treatment effect of treating gallium ions in wastewater by using deinococcus radiodurans in an application example.
FIG. 4 is a graph showing the effect of treating aluminum ions in wastewater using deinococcus radiodurans in an example of application.
FIG. 5 is a graph showing the effect of treating indium ions in wastewater using deinococcus radiodurans in an example of application.
Detailed Description
The invention is described in further detail below with reference to the figures and the embodiments.
FIG. 1 is a flow chart illustrating a method of biological agent removal of metal ions according to an exemplary embodiment. As shown in fig. 1, the method includes:
In the present disclosure, Deinococcus Radiodurans (DR) is a non-pathogenic bacterium with superior radiation resistance. Their superior radiation resistance benefits mainly from: (1) (ii) multiple copies of a genomic sequence; (2) a compact chromosomal organisation; (3) high intracellular concentration of ferromanganese ion ratio; (4) carotenoids synthesized in vivo in large quantities; (5) 3 catalase and 4 superoxide dismutase codes; sixthly, a powerful DNA damage repairing system. This makes deinococcus radiodurans resistant to acute exposure of 15kGy joules per kilogram.
In one possible implementation, the method further includes: before the dry bacterial powder is added into the liquid to be treated, the hydrogen ion concentration index of the liquid to be treated is between 2.5 and 5.5.
In one possible implementation, the incubation treatment of the liquid to be treated containing a predetermined concentration of deinococcus radiodurans includes:
arranging a liquid to be treated containing deinococcus radiodurans with preset concentration on a constant-temperature shaking table;
and enabling the constant-temperature shaking table to continuously rotate at a preset rotating speed for a preset time.
Wherein the temperature of the constant temperature shaking table is between 16 and 30 ℃, the preset rotating speed is between 100 and 200 revolutions per minute, and the preset time duration is between 2 and 5 hours.
In one possible implementation manner, the metal ions contained in the liquid to be treated include any one or more of aluminum ions, gallium ions, vanadium ions, or indium ions.
In an application example, the following description is given by taking the example of removing vanadium ions in a liquid:
to demonstrate the ability of deinococcus radiodurans to act as a biological remover of metal vanadium ions, the following experiments were conducted in the laboratory:
coli K12(e.coli, Escherichia coli) was used as an experimental control. Since Escherichia coli K12 is a non-toxic microorganism, it is often used as a model organism in daily studies.
Taking four to-be-treated wastewater samples with the same vanadium ion concentration (the vanadium ion concentration is 191.757 mu g/L), and adding deinococcus radiodurans into the first wastewater sample to ensure that the concentration of the deinococcus radiodurans in the first wastewater sample is 0.8 g/L; adding deinococcus radiodurans into the second wastewater sample to enable the concentration of the deinococcus radiodurans in the second wastewater sample to be 1.6 g/L; adding escherichia coli into the third wastewater sample to enable the concentration of the escherichia coli in the third wastewater sample to be 0.4 g/L; adding escherichia coli into the fourth wastewater sample to enable the concentration of the escherichia coli in the fourth wastewater sample to be 0.8 g/L; FIG. 2 is a graph showing the effect of treating vanadium ions in wastewater using deinococcus radiodurans in an application example. As shown in fig. 2, the vanadium ions in each wastewater sample finally reach a dynamic equilibrium with time, and even the concentration of the metal vanadium ions is higher than that in the previous period, probably due to the re-dissolution after the microbial treatment is saturated. After 2.5 hours of treatment, the concentration of vanadium ions in the first wastewater sample was 42.411 μ g/L, the concentration of vanadium ions in the second wastewater sample was 12.51 μ g/L, the concentration of vanadium ions in the third wastewater sample was 187.642 μ g/L, and the concentration of vanadium ions in the fourth wastewater sample was 194.853 μ g/L. Therefore, the efficiency of removing the vanadium ions in the wastewater by using the deinococcus radiodurans can be up to 93.5%, and the effect of removing the vanadium ions by using the deinococcus radiodurans is far higher than that of removing the vanadium ions by using escherichia coli.
In an application example, the following description is given by taking the removal of gallium ions in a liquid as an example:
taking four to-be-treated wastewater samples with the same gallium ion concentration (the gallium ion concentration is 187.073 mu g/L), and adding deinococcus radiodurans into the first wastewater sample to ensure that the concentration of the deinococcus radiodurans in the first wastewater sample is 0.8 g/L; adding deinococcus radiodurans into the second wastewater sample to enable the concentration of the deinococcus radiodurans in the second wastewater sample to be 1.6 g/L; adding escherichia coli into the third wastewater sample to enable the concentration of the escherichia coli in the third wastewater sample to be 0.4 g/L; adding escherichia coli into the fourth wastewater sample to enable the concentration of the escherichia coli in the fourth wastewater sample to be 0.8 g/L; FIG. 3 is a schematic diagram showing the treatment effect of treating gallium ions in wastewater by using deinococcus radiodurans in an application example. As shown in fig. 3, the gallium ions in each wastewater sample finally reach a dynamic equilibrium with time, and even the concentration of the gallium ions is higher than that in the previous period, which may be caused by the re-dissolution after the microbial treatment is saturated. After 2.5 hours of treatment, the concentration of gallium ions in the first wastewater sample was 14.412 μ g/L, the concentration of gallium ions in the second wastewater sample was 9.154 μ g/L, the concentration of gallium ions in the third wastewater sample was 165.842 μ g/L, and the concentration of gallium ions in the fourth wastewater sample was 143.51 μ g/L. Therefore, the efficiency of removing the gallium ions in the wastewater by using deinococcus radiodurans can be as high as 95.11%, and the effect of removing the gallium ions by using deinococcus radiodurans is far higher than that of removing the gallium ions by using escherichia coli.
In an application example, the following description is given by taking the removal of aluminum ions in a liquid as an example:
taking four to-be-treated wastewater samples with the same aluminum ion concentration (the aluminum ion concentration is 212.816 mu g/L), and adding deinococcus radiodurans into the first wastewater sample to ensure that the concentration of deinococcus radiodurans in the first wastewater sample is 0.8 g/L; adding deinococcus radiodurans into the second wastewater sample to enable the concentration of the deinococcus radiodurans in the second wastewater sample to be 1.6 g/L; adding escherichia coli into the third wastewater sample to enable the concentration of the escherichia coli in the third wastewater sample to be 0.4 g/L; adding escherichia coli into the fourth wastewater sample to enable the concentration of the escherichia coli in the fourth wastewater sample to be 0.8 g/L; FIG. 4 is a graph showing the effect of treating aluminum ions in wastewater using deinococcus radiodurans in an example of application. As shown in fig. 4, with time, the aluminum ions in each wastewater sample finally reach a dynamic equilibrium, and even the concentration of the metal aluminum ions is higher than that in the previous time period, or even the concentration of the metal aluminum ions is higher than that in the previous time period, which may be caused by re-dissolution after the microbial treatment is saturated; on the other hand, the Escherichia coli powder is internally provided with aluminum ions due to the existence of the aluminum ions in the LB culture medium for culturing the Escherichia coli. After 2.5 hours of treatment, the concentration of aluminum ions in the first wastewater sample was 13.918 μ g/L, the concentration of aluminum ions in the second wastewater sample was 6.49 μ g/L, the concentration of aluminum ions in the third wastewater sample was 248.995 μ g/L, and the concentration of aluminum ions in the fourth wastewater sample was 221.162 μ g/L. Therefore, the efficiency of removing the aluminum ions in the wastewater by using deinococcus radiodurans can be as high as 96.95%, and the effect of removing the aluminum ions by using deinococcus radiodurans is far higher than that of removing the aluminum ions by using escherichia coli.
In an application example, the following description is given by taking the example of removing indium ions in a liquid:
taking four wastewater samples to be treated with the same indium ion concentration (the indium ion concentration is 189.07 mu g/L), and adding deinococcus radiodurans into the first wastewater sample to ensure that the concentration of deinococcus radiodurans in the first wastewater sample is 0.8 g/L; adding deinococcus radiodurans into the second wastewater sample to enable the concentration of the deinococcus radiodurans in the second wastewater sample to be 1.6 g/L; adding escherichia coli into the third wastewater sample to enable the concentration of the escherichia coli in the third wastewater sample to be 0.4 g/L; adding escherichia coli into the fourth wastewater sample to enable the concentration of the escherichia coli in the fourth wastewater sample to be 0.8 g/L; FIG. 4 is a graph showing the effect of treating indium ions in wastewater using deinococcus radiodurans in an example of application. As shown in fig. 4, the indium ions in each wastewater sample finally reach a dynamic equilibrium with time, and even the concentration of the metal indium ions is higher than that in the previous period, which may be caused by the re-dissolution after the microbial treatment is saturated. After 2.5 hours of treatment, the concentration of indium ions in the first wastewater sample was 1.138. mu.g/L, the concentration of indium ions in the second wastewater sample was 0.723. mu.g/L, the concentration of indium ions in the third wastewater sample was 45.293. mu.g/L, and the concentration of indium ions in the fourth wastewater sample was 19.607. mu.g/L. Therefore, the efficiency of removing the indium ions in the wastewater by using deinococcus radiodurans can be up to 99.62%, and the effect of removing the indium ions by using deinococcus radiodurans is far higher than that of removing the indium ions by using escherichia coli.
In one possible implementation, there is provided a method of preparing a biological agent, the method comprising:
inoculating deinococcus radiodurans into TGY medium, culturing on a constant temperature (e.g. 30 deg.C) shaking table, wherein the deinococcus radiodurans has a cell density of 1.0-1.5 (e.g. OD)6001.50), the medium was collected by a centrifuge, and the intermediate obtained by the centrifugation was freeze-dried in a freeze-dryer to obtain the dry fungal powder described above.
Having described embodiments of the present disclosure, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terms used herein were chosen in order to best explain the principles of the embodiments, the practical application, or technical improvements to the techniques in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (8)
1. A method for removing metal ions from a biological agent, the method comprising:
continuously adding dried powder of deinococcus radiodurans with biological activity into liquid to be treated containing metal ions until the concentration of deinococcus radiodurans in the liquid to be treated reaches a preset concentration, wherein the preset concentration is between 0.8 and 1.6 grams per liter;
and incubating the liquid to be treated containing deinococcus radiodurans with preset concentration so as to reduce the concentration of metal ions in the liquid to be treated.
2. The method of claim 1, further comprising:
before the dry bacterial powder is added into the liquid to be treated, the hydrogen ion concentration index of the liquid to be treated is between 2.5 and 5.5.
3. The method according to claim 1, wherein the incubation treatment of the liquid to be treated containing a predetermined concentration of deinococcus radiodurans comprises:
arranging a liquid to be treated containing deinococcus radiodurans with preset concentration on a constant-temperature shaking table;
and enabling the constant-temperature shaking table to continuously rotate at a preset rotating speed for a preset time.
4. The method according to claim 3, wherein the temperature of the constant temperature rocking bed is between 16 and 30 degrees Celsius.
5. The method as claimed in claim 3, wherein the predetermined rotation speed is between 100 and 200 revolutions per minute.
6. The method of claim 3, wherein the predetermined period of time is between 2-5 hours.
7. The method according to claim 1, wherein the liquid to be treated contains metal ions including any one or more of aluminum ions, gallium ions, vanadium ions, or indium ions.
8. A method of preparing a biological agent, the method comprising:
inoculating deinococcus radiodurans to a culture medium, culturing on a constant-temperature shaking table, collecting the culture medium by using a centrifugal machine under the condition that the cell density of deinococcus radiodurans in the culture medium is between 1.0 and 1.5, and freeze-drying an intermediate product obtained by centrifugal treatment in a freeze-drying machine to obtain the dry bacterium powder.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103112955A (en) * | 2013-03-11 | 2013-05-22 | 浙江省农业科学院 | Method for degrading and decolorizing malachite green wastewater by utilizing deinococcus radiodurans R1 |
| CN105498718A (en) * | 2016-02-01 | 2016-04-20 | 南华大学 | Biological surfactant functional modification method for deinococcus radiodurans (DR) and application thereof |
| KR101806296B1 (en) * | 2016-11-11 | 2017-12-07 | 서울시립대학교 산학협력단 | Deinococcus radiodurans Having Improved Ability of Removing Radioactive Iodine and Method for Removing Radioactive Iodine Using the Same |
| US20210012916A1 (en) * | 2019-07-09 | 2021-01-14 | Coenbio Co., Ltd. | Composition for converting radioactive substance into non-radioactive substance and a method of preparing the composition |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103112955A (en) * | 2013-03-11 | 2013-05-22 | 浙江省农业科学院 | Method for degrading and decolorizing malachite green wastewater by utilizing deinococcus radiodurans R1 |
| CN105498718A (en) * | 2016-02-01 | 2016-04-20 | 南华大学 | Biological surfactant functional modification method for deinococcus radiodurans (DR) and application thereof |
| KR101806296B1 (en) * | 2016-11-11 | 2017-12-07 | 서울시립대학교 산학협력단 | Deinococcus radiodurans Having Improved Ability of Removing Radioactive Iodine and Method for Removing Radioactive Iodine Using the Same |
| US20210012916A1 (en) * | 2019-07-09 | 2021-01-14 | Coenbio Co., Ltd. | Composition for converting radioactive substance into non-radioactive substance and a method of preparing the composition |
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