CN114199768A - Line scanning confocal reflective imaging device - Google Patents
Line scanning confocal reflective imaging device Download PDFInfo
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- CN114199768A CN114199768A CN202111510535.XA CN202111510535A CN114199768A CN 114199768 A CN114199768 A CN 114199768A CN 202111510535 A CN202111510535 A CN 202111510535A CN 114199768 A CN114199768 A CN 114199768A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 58
- 230000003287 optical effect Effects 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 7
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- 238000010586 diagram Methods 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010226 confocal imaging Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
- G02B26/10—Scanning systems
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Abstract
The invention discloses a line scanning confocal reflection type imaging device, which comprises: the device comprises an eyepiece, a scanning lens, a scanning galvanometer, a beam splitter, a cylindrical mirror, a slit, a linear array camera, a laser and an imaging device; after laser emitted by the laser passes through the cylindrical lens, a point light source is converted into a line light source, then the line light source transmits the beam splitter to the scanning galvanometer, and then the laser passes through the scanning lens and the ocular lens in sequence and reaches the surface of a sample; and light reflected by the sample is reflected by the beam splitter after passing through the ocular lens, the scanning lens and the scanning galvanometer in sequence, is collected by the linear array camera after passing through the slit, and is imaged in the imaging device. After the point light source is converted into the line light source through the cylindrical mirror, the linear array camera is used for collecting sample reflected light under the scanning action of the high-speed galvanometer, so that the rapid reflection imaging can be realized; the invention provides a darkroom imaging environment by arranging the shading mechanism capable of being electrically controlled, can improve the imaging effect and is convenient to operate.
Description
Technical Field
The invention relates to the field of line scanning confocal imaging, in particular to a line scanning confocal reflective imaging device.
Background
The confocal imaging technology filters stray light of a non-focusing layer by using a small hole or a slit, only detects light of a focusing layer of an objective lens, and has the characteristic of high resolution. However, the existing solutions often adopt a point-scanning imaging mode, which requires scanning the sample point by point, and the imaging speed is greatly limited.
Therefore, there is a need to provide a more reliable solution.
Disclosure of Invention
The present invention is directed to a line scanning confocal reflective imaging apparatus, which overcomes the above-mentioned shortcomings in the prior art.
In order to solve the technical problems, the invention adopts the technical scheme that: a line-scanning confocal reflective imaging apparatus, comprising: the device comprises an eyepiece, a scanning lens, a scanning galvanometer, a beam splitter, a cylindrical mirror, a slit, a linear array camera, a laser and an imaging device;
after laser emitted by the laser passes through the cylindrical lens, a point light source is converted into a line light source, then the line light source transmits the beam splitter to the scanning galvanometer, and then the laser passes through the scanning lens and the ocular lens in sequence and reaches the surface of a sample;
and light reflected by the sample is reflected by the beam splitter after passing through the ocular lens, the scanning lens and the scanning galvanometer in sequence, is collected by the linear array camera after passing through the slit, and is imaged in the imaging device.
Preferably, the line scanning confocal reflective imaging device further comprises an optical platform, and the eyepiece, the scanning lens, the scanning galvanometer, the beam splitter, the cylindrical mirror, the slit, the line camera and the laser are all arranged on the optical platform through an installation frame.
Preferably, a sample holder for placing a sample is further arranged on the optical platform.
Preferably, the imaging device is a computer.
Preferably, the line scanning confocal reflective imaging device further comprises a shading mechanism arranged on the optical platform.
Preferably, the sample holder, the eyepiece, the scanning lens, the scanning galvanometer, the spectroscope, the cylindrical mirror, the slit, the line camera and the laser are all arranged in a containing cavity formed between the shading mechanism and the optical platform, and the containing cavity is used for providing a darkroom imaging environment.
Preferably, the shading mechanism comprises a box body which is arranged on the optical platform and is open at the upper part and the lower part, a cover plate which is rotatably arranged on the box body and a driving component which is used for driving the cover plate to rotate;
the box body and the optical platform surround to form the accommodating cavity, and the cover plate is used for sealing the upper opening of the accommodating cavity.
Preferably, the driving assembly comprises a U-shaped driving rod connected to the outer surface of the cover plate, a rotating shaft rotatably arranged on the back plate of the box body and connected with the U-shaped driving rod, and a motor for driving the rotating shaft to rotate;
the first end of the U-shaped driving rod is fixedly connected with the outer surface of the cover plate, and the second end of the U-shaped driving rod is fixedly connected with the rotating shaft.
Preferably, a support is connected to the back plate of the box body, two bearings are arranged on the support, and the rotating shaft is rotatably arranged on the two bearings.
Preferably, the bracket is further connected with a limiting mounting plate, the limiting mounting plate is in threaded connection with a limiting screw, the limiting screw is located below the second end of the U-shaped driving rod, and the second end of the U-shaped driving rod is jacked from bottom to top to limit the rotating position of the cover plate.
The invention has the beneficial effects that:
according to the line scanning confocal reflection type imaging device, after a point light source is converted into a line light source through the cylindrical mirror, the linear array camera is used for collecting sample reflection light through the scanning effect of the high-speed galvanometer, and rapid reflection imaging can be achieved;
the invention provides a darkroom imaging environment by arranging the shading mechanism capable of being electrically controlled, can improve the imaging effect and is convenient to operate.
Drawings
Fig. 1 is a schematic structural diagram of a line-scanning confocal reflective imaging apparatus according to embodiment 1 of the present invention;
fig. 2 is a schematic structural diagram of a line-scanning confocal reflective imaging apparatus according to embodiment 1 of the present invention with part of components removed;
fig. 3 is an optical path diagram of a line-scanning confocal reflective imaging apparatus according to embodiment 1 of the present invention;
fig. 4 is a schematic structural diagram of a slit and a line camera in embodiment 1 of the present invention;
fig. 5 is a schematic structural diagram of a line-scanning confocal reflective imaging apparatus according to embodiment 2 of the present invention;
fig. 6 is a schematic structural view of a front part of a light shield mechanism in embodiment 2 of the present invention;
fig. 7 is a schematic structural view of the back of the light shielding mechanism in embodiment 2 of the present invention;
fig. 8 is another view of the back of the shade mechanism in embodiment 2 of the present invention.
Description of reference numerals:
10-sample; 11-ocular lens; 12-a scanning lens; 13-scanning galvanometer; 14-a light splitting sheet; 15-cylindrical mirror; 16-a laser; 17-a slit; 18-a line camera; 19-an imaging device;
2-an optical bench; 20-a mounting frame; 21-sample holder;
3-a shading mechanism; 30, a box body; 31-cover plate; 32-a drive assembly; 33-a back plate; 34-a bracket; 35-a bearing; 36-limiting mounting plate; 37-limit screw; 38-a flexible member; 39-sealing ring; 320-U-shaped driving rod; 321-a rotating shaft; 322-motor; 3201 — first end; 3202 — second end;
4-containing cavity.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
As shown in fig. 1-4, a line-scanning confocal reflective imaging apparatus 19 of the present embodiment includes: an eyepiece 11, a scanning lens 12, a scanning galvanometer 13, a beam splitter 14, a cylindrical mirror 15, a slit 17, a line camera 18, a laser 16 and an imaging device 19;
after laser emitted by a laser 16 passes through a cylindrical mirror 15, a point light source is converted into a line light source, then the line light source transmits through a beam splitter 14 to a scanning galvanometer 13, and then the line light source passes through a scanning lens 12 and an eyepiece 11 in sequence and reaches the surface of a sample 10;
the light reflected by the sample 10 passes through the ocular lens 11, the scanning lens 12 and the scanning galvanometer 13 in sequence, is reflected by the beam splitter 14, passes through the slit 17, is collected by the linear array camera 18, and is finally imaged in the imaging device 19.
In this embodiment, the scanning galvanometer 13 is a high-speed scanning galvanometer 13.
In this embodiment, the line-scanning confocal reflective imaging apparatus 19 further includes an optical platform 2, and the eyepiece 11, the scanning lens 12, the scanning galvanometer 13, the beam splitter 14, the cylindrical mirror 15, the slit 17, the line camera 18, and the laser 16 are all disposed on the optical platform 2 through the mounting frame 20. Further, a sample holder 21 for holding the sample 10 is disposed on the optical platform 2.
In this embodiment, the imaging device 19 is a computer.
In one embodiment, the line-scanning confocal reflective imaging apparatus 19 operates according to the following principle:
turning on a laser 16, adjusting power to a safe range, controlling a scanning galvanometer 13 and a linear array camera 18 through a computer, enabling the laser 16 to emit laser, converting a point light source into a linear light source through a cylindrical mirror 15, transmitting a beam splitter 14 to the scanning galvanometer 13, and sequentially passing through a scanning lens 12 and an ocular 11 to reach the surface of a sample 10;
light reflected by the sample 10 passes through the ocular lens 11, the scanning lens 12 and the scanning galvanometer 13 in sequence, is reflected by the beam splitter 14, is filtered by the slit 17 to remove stray light and then is collected by the linear array camera 18, and signals collected by the linear array camera 18 are transmitted to the imaging device 19 for reflection imaging.
After the point light source is converted into the line light source through the cylindrical mirror 15, the linear array camera 18 is used for collecting sample reflected light under the scanning action of the high-speed galvanometer, and finally, quick reflection imaging can be realized.
Example 2
Referring to fig. 5-8, the line-scanning confocal reflective imaging apparatus 19 of the present invention can have better imaging effect in a dark room, and as a further improvement on embodiment 1, the line-scanning confocal reflective imaging apparatus 19 of the present embodiment further includes a light shielding mechanism 3 disposed on the optical platform 2. The shading mechanism 3 is an electric control mechanism, can improve the darkroom environment required by realizing good imaging, and is convenient to control.
Specifically, in the present embodiment, the sample holder 21, the eyepiece 11, the scanning lens 12, the scanning galvanometer 13, the beam splitter 14, the cylindrical mirror 15, the slit 17, the line camera 18, and the laser 16 are all disposed in the accommodating cavity 4 formed between the light shielding mechanism 3 and the optical platform 2, and the accommodating cavity 4 is used for providing a darkroom imaging environment. The shading mechanism 3 comprises a box body 30 which is arranged on the optical platform 2 and is open at the upper part and the lower part, a cover plate 31 which is rotatably arranged on the box body 30 and a driving component 32 which is used for driving the cover plate 31 to rotate; the box body 30 and the optical platform 2 surround to form a containing cavity 4, and the cover plate 31 is used for sealing the upper opening of the containing cavity 4.
The driving assembly 32 includes a U-shaped driving rod 320 connected to the outer surface of the cover plate 31, a rotating shaft 321 rotatably disposed on the back plate 33 of the box body 30 and connected to the U-shaped driving rod 320, and a motor 322 for driving the rotating shaft 321 to rotate; the first end 3201 of the U-shaped driving rod 320 is fixedly connected to the outer surface of the cover plate 31, and the second end 3202 of the U-shaped driving rod 320 is fixedly connected to the rotating shaft 321.
Wherein, the back plate 33 of the box body 30 is connected with a bracket 34, two bearings 35 are arranged on the bracket 34, and the rotating shaft 321 is rotatably arranged on the two bearings 35.
In the preferred embodiment, the bracket 34 is further connected to a limiting mounting plate 36, the limiting mounting plate 36 is threadedly connected to a limiting screw 37, the limiting screw 37 is located below the second end 3202 of the U-shaped driving rod 320, and the rotating position of the cover plate 31 is limited by pressing the second end 3202 of the U-shaped driving rod 320 from bottom to top.
In the preferred embodiment, the upper portion of the box body 30 is further provided with a sealing ring 39, which is beneficial to improve the sealing effect between the cover plate 31 and the box body 30. A flexible member 38, such as a flexible cloth or a flexible sheet, is further connected between the cover plate 31 and the box body 30, and when the cover plate 31 is completely opened (as shown in fig. 6, the cover plate 31 is substantially vertical), the flexible member 38 is straightened and unfolded to support and limit the completely opened posture of the cover plate 31.
In one embodiment, the operation principle of the shade mechanism 3 is:
after the sample 10 is placed on the sample holder 21 and ready, the motor 322 works to drive the rotating shaft 321 to rotate, and the rotating shaft 321 drives the U-shaped driving rod 320 to rotate, so as to drive the cover plate 31 to rotate and be buckled on the box body 30, thereby realizing sealing and imaging in a dark room; when the cover plate 31 is opened after imaging is completed, the motor 322 rotates reversely, the cover plate 31 is driven to open by the U-shaped driving rod 320, when the cover plate 31 is completely opened, the flexible part 38 is straightened, and the second end 3202 of the U-shaped driving rod 320 is pressed against the limiting screw 37 to limit the cover plate 31.
While embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of application of the invention, and further modifications may readily be effected by those skilled in the art, so that the invention is not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.
Claims (10)
1. A line-scan confocal reflective imaging apparatus, comprising: the device comprises an eyepiece, a scanning lens, a scanning galvanometer, a beam splitter, a cylindrical mirror, a slit, a linear array camera, a laser and an imaging device;
after laser emitted by the laser passes through the cylindrical lens, a point light source is converted into a line light source, then the line light source transmits the beam splitter to the scanning galvanometer, and then the laser passes through the scanning lens and the ocular lens in sequence and reaches the surface of a sample;
and light reflected by the sample is reflected by the beam splitter after passing through the ocular lens, the scanning lens and the scanning galvanometer in sequence, is collected by the linear array camera after passing through the slit, and is imaged in the imaging device.
2. The line-scanning confocal reflective imaging apparatus according to claim 1, further comprising an optical platform, wherein the eyepiece, the scanning lens, the scanning galvanometer, the beam splitter, the cylindrical mirror, the slit, the line camera, and the laser are all disposed on the optical platform through a mounting frame.
3. The line-scanning confocal reflective imaging apparatus according to claim 2, wherein a sample holder for placing a sample is further disposed on the optical platform.
4. The line-scanning confocal reflective imaging device of claim 1, wherein the imaging device is a computer.
5. The line-scanning confocal reflective imaging apparatus according to claim 2, further comprising a light-blocking mechanism disposed on the optical platform.
6. The line-scanning confocal reflective imaging apparatus according to claim 5, wherein the sample holder, the eyepiece, the scanning lens, the scanning galvanometer, the beam splitter, the cylindrical mirror, the slit, the line camera, and the laser are all disposed in a receiving cavity formed between the light shielding mechanism and the optical platform, and the receiving cavity is used for providing a darkroom imaging environment.
7. The line-scanning confocal reflective imaging apparatus according to claim 6, wherein the light shielding mechanism comprises a box disposed on the optical platform and having an upper opening and a lower opening, a cover rotatably disposed on the box, and a driving assembly for driving the cover to rotate;
the box body and the optical platform surround to form the accommodating cavity, and the cover plate is used for sealing the upper opening of the accommodating cavity.
8. The line-scanning confocal reflective imaging apparatus according to claim 7, wherein the driving assembly comprises a U-shaped driving rod connected to an outer surface of the cover plate, a rotating shaft rotatably disposed on the back plate of the box body and connected to the U-shaped driving rod, and a motor for driving the rotating shaft to rotate;
the first end of the U-shaped driving rod is fixedly connected with the outer surface of the cover plate, and the second end of the U-shaped driving rod is fixedly connected with the rotating shaft.
9. The line-scanning confocal reflective imaging apparatus according to claim 8, wherein a support is connected to the back plate of the box, two bearings are disposed on the support, and the rotating shaft is rotatably disposed on the two bearings.
10. The line-scanning confocal reflective imaging apparatus according to claim 9, wherein a limit mounting plate is further connected to the bracket, a limit screw is threadedly connected to the limit mounting plate, the limit screw is located below the second end of the U-shaped driving rod, and the limit of the rotational position of the cover plate is achieved by pressing the second end of the U-shaped driving rod from bottom to top.
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CN202111510535.XA CN114199768A (en) | 2021-12-10 | 2021-12-10 | Line scanning confocal reflective imaging device |
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CN202111510535.XA CN114199768A (en) | 2021-12-10 | 2021-12-10 | Line scanning confocal reflective imaging device |
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CN213338166U (en) * | 2020-11-05 | 2021-06-01 | 武汉中弘精密机械有限公司 | Optical platform shading device |
CN217385177U (en) * | 2021-12-10 | 2022-09-06 | 济南国科医工科技发展有限公司 | Line scanning confocal reflective imaging device |
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2021
- 2021-12-10 CN CN202111510535.XA patent/CN114199768A/en active Pending
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US6545264B1 (en) * | 1998-10-30 | 2003-04-08 | Affymetrix, Inc. | Systems and methods for high performance scanning |
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KR20060033830A (en) * | 2004-10-16 | 2006-04-20 | 학교법인연세대학교 | Confocal laser--line scanning microscope with acousto-optic deflector and line scan camera |
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