CN114196594A

CN114196594A - Sphingobacterium canadensis SC202103 strain for degrading alpha-solanine and application thereof

Info

Publication number: CN114196594A
Application number: CN202111666246.9A
Authority: CN
Inventors: 陈斌; 王文倩; 肖关丽; 杜广祖; 叶举辉; 杨宝云; 薛锐; 张栩; 孙淦琳; 付玉飞
Original assignee: Yunnan Agricultural University
Current assignee: Yunnan Agricultural University
Priority date: 2021-12-31
Filing date: 2021-12-31
Publication date: 2022-03-18
Anticipated expiration: 2041-12-31
Also published as: CN114196594B

Abstract

The invention discloses a sphingosine canadensis Sphingobacterium canadensis strain for degrading alpha-solanine and application thereof, the SC202103 strain for degrading alpha-solanine is SC202103, and the preservation name is sphingosine canadensis Sphingobacterium canadensis SC 202103; is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation address is No. 3 of Xilu No.1 of Beijing, Chaoyang district; the preservation date is as follows: 31/5/2021; the preservation number is: CGMCC No. NO22640. The strain is a bacterium which can be used for degrading alpha-solanine, has the characteristics of strong degradation capability on the alpha-solanine and environmental safety, and can be used for biologically degrading the alpha-solanine and ensuring food safety.

Description

Sphingobacterium canadensis SC202103 strain for degrading alpha-solanine and application thereof

Technical Field

The invention relates to the technical field of microorganisms, in particular to a sphingosine canadensis SC202103 strain for degrading alpha-solanine and a preparation method and application thereof.

Background

Solanine (Solanine), also known as Solanine, pototoxin, etc., is a class of glycoside alkaloids (SGAs), secondary metabolites that are mainly distributed in solanaceae plants, such as roots, stems, and leafy fruits of potatoes, tomatoes, and eggplants, wherein the content is abundant in potatoes (Friedman and McDonald, 1997; zhao snow et al, 2007). The alpha-solanine in the potato has insecticidal activity (Tingey, 1984; Jonasson and Olsson, 1994; Zheng et al, 2020), has high toxicity to human and livestock, can cause symptoms such as nausea, vomiting, diarrhea, loss of consciousness, paralysis, shock and the like when being eaten by mistake, and can cause death if the rescue is not carried out in time, and also has strong teratogenic and reproductive toxicity (Yusain, 2009; Tansjun, 2018).

The main means for degrading alpha-solanine are acid hydrolysis or active enzyme hydrolysis (Friedman et al, 1993; Weltring et al, 1997), such as the alpha-solanine degrading activity of some enzymes extracted from some potatoes (Nikolic and Stankovic, 2005; Dahlin et al, 2017), which can degrade by the gradual removal of the carbohydrate fraction. However, the removal of solanine by acid hydrolysis or extraction of enzymes from plants is cumbersome, time consuming, labor intensive, costly and presents a potential risk of environmental pollution. Therefore, the need for novel and efficient solanine degrading bacteria is an important way for solanine clearing in the field of potato food. Some researchers have begun to isolate solanine-degrading bacteria from some special environments. For example, Jensen et al (2009) finds that microorganisms in underground water can hydrolyze alpha-solanine to remove sugar molecules one by one, can generate beta-solanine, gamma-solanine and solanidine, and the toxicity of the beta-solanine, the gamma-solanine and the solanidine is also reduced in sequence, but the classification status of the microorganisms is not clear; hennessy et al (2018, 2020) isolated bacteria of Arthrobacter and Serratia from the soil surrounding green-skinned potatoes have highly effective degradation of the glycoside alkaloid alpha-solanine in potatoes. However, there are few reports on the domestic excavation of solanine degrading bacteria resources.

Sphingobacterium canadensis, belongs to the Bacteroides, family sphingobacteriaceae, genus Sphingobacterium. Mehnaz et al (2007) reported that this bacterium could hydrolyze esculin, gelatin and urease, and have an assimilation effect on arabinose, melibiose and glycerol. Mehnaz et al (2010) and Ali et al (2015) reported that this bacterium was able to synthesize indoleacetic acid IAA, promoting the growth of maize and chickpea (Cicer arietinum L). However, at present, few reports on other functions of the strain are reported at home and abroad. The Sphingobacterium canadensis SC202103 separated from the tuber moth of the potato in the research has a high-efficiency degradation effect on the alpha-solanine, and the degradation efficiency is as high as 99.79%.

The tuber moth Phthyrima operculell (Zeller) belongs to insects in the genus of Pseudoplutella of the superfamily Conidiobolus of Diploptera, and is a solanaceous crop pest such as potatoes, tobaccos, tomatoes, peppers and the like which are distributed globally. The intestinal tract of the tuber moth contains abundant intestinal bacteria which play an important role in host adaptation and metabolism of the tuber moth. Research reports that symbiotic bacteria in insect intestinal tracts have the effect of degrading host plant alkaloid, and symbiotic serratia in potato tuber moth intestinal tracts have the effect of degrading potato glucoside alkaloid alpha-solanine. The alpha-solanine is degraded by using the Sphingobacterium canadensis SC202103 strain separated from intestinal tracts of tuber moth larvae of potatoes, so that the method provides an alternative strain for safely and effectively removing the solanine in foods developed by taking the potatoes as raw materials, and has important practical application value.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a sphingosine canadensis SC202103 strain for degrading alpha-solanine and application thereof.

In order to solve the problems in the prior art, the invention provides the following technical scheme: the invention relates to a sphingosine canadensis SC202103 strain for degrading alpha-solanine, which is sphingosine canadensis with a preservation name of sphingosine canadensis Sphingobacterium canadensis; is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation address is No. 3 of Xilu No.1 of Beijing, Chaoyang district; the preservation date is as follows: 31/5/2021; the preservation number is: CGMCC No. 22640.

Further, the gene sequence of the 16S rDNA of the alpha-solanine degrading Sphingobacterium canadensis SC202103 strain is the nucleotide sequence shown in SEQ ID No. 1. The strain is identified as Sphingobacterium canadensis through sequencing.

The bacterial colony of the strain S1 is round, neat in edge, orange yellow, smooth and moist in surface, slightly convex in center and flat in periphery after being subjected to LB plate streak purification culture. The cells are red by gram staining, gram-negative bacteria and short rod-shaped.

The invention relates to a Sphingobacterium canadensis SC202103 strain microbial inoculum prepared from the Sphingobacterium canadensis SC202103 strain.

Further, the active ingredient is at least one of the following (a), (b) and (c):

(a) a primary extract of a fermentation broth of said sphingosine canadensis strain SC 202103;

(b) ultrasonically lysing the supernatant of the cells of the obtained sphingosine canadensis SC202103 strain;

(c) ultrasonic lysis of the cells of the resulting Sphingobacterium canadensis strain SC 202103.

The preparation method of the sphingosine canadensis SC202103 strain microbial inoculum comprises the following steps:

(1) separating from the collected midgut of the potato tuber moth larvae to obtain a wild Sphingobacterium canadensis SC202103 strain, culturing on an LB culture medium, and transferring the strain to a newly prepared LB culture medium for purification;

(2) then purified and cultured to obtain purified sphingosine canadensis SC 202103;

(3) the alpha-solanine degradation capability is measured by adopting the extracellular enzyme of the high-efficiency degradation strain, and the degradation rate of the extracellular enzyme of the strain to the alpha-solanine reaches 99.79 percent.

The invention relates to an application of a Sphingobacterium canadensis SC202103 strain in an alpha-solanine degradation preparation.

Has the advantages that: the sphingosine canadensis SC202103 provided by the invention is a degrading bacterium which can be used for degrading toxic alpha-solanine of solanaceae plants, has the characteristics of strong degrading capability and environmental safety, and can be used for biologically degrading the alpha-solanine.

Compared with the prior art, the invention has the following advantages: (1) the isolated sphingosine canadensis strain SC202103 is a bacterial strain with a strong degrading effect on sphingosine canadensis SC 202103.

(2) The Sphingobacterium canadensis SC202103 strain is simple to culture, grows quickly and is easy to culture and propagate in a large scale indoors.

(3) The sphingosine canadensis SC202103 provided by the invention is a pest intestinal bacterium which can be used for green degradation of alpha-solanine, can improve the adaptability of tuber moths to solanaceae plants, can efficiently degrade the alpha-solanine, has the characteristics of strong degradation effect and no environmental pollution, and can be widely used for biodegradation of the alpha-solanine.

Drawings

FIG. 1 is a cell morphology of Sphingobacterium canadensis SC202103 of the present invention.

FIG. 2 is a graph showing the growth of Sphingobacterium canadensis SC202103 according to the present invention.

Detailed Description

The following embodiments are described in detail with reference to the accompanying drawings, so that how to implement the technical features of the present invention to solve the technical problems and achieve the technical effects can be fully understood and implemented.

Example 1

The invention relates to a sphingosine canadensis SC202103 strain for degrading alpha-solanine, which is sphingosine canadensis with a preservation name of Glutamicibacter halophytocala; is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation address is No. 3 of Xilu No.1 of Beijing, Chaoyang district; the preservation date is as follows: 31/5/2021; the preservation number is: CGMCC No. 22640.

The gene sequence of the 16S rDNA of the sphingosine canadensis SC202103 strain for degrading alpha-solanine is the nucleotide sequence shown in SEQ ID No. 1.

The bacterial strain is purified and cultured by an LB plate streak plate, and the bacterial colony of the bacterial strain S1 is round, neat in edge, orange yellow, smooth and moist in surface, slightly convex in center and flat in periphery. The cells are red by gram staining, gram-negative bacteria and short rod-shaped.

The invention relates to a Sphingobacterium canadensis GH202103 strain microbial inoculum prepared from a Sphingobacterium canadensis SC202103 strain.

The active component is at least one of the following components (a), (b) and (c):

The preparation method of the sphingosine canadensis SC202103 microbial inoculum comprises the following steps:

(1) separating wild Sphingobacterium canadensis SC202103 strain from midgut of field potato tuber moth larva, culturing on LB culture medium, transferring the strain to newly prepared LB culture medium for purification;

The invention relates to an application of a Sphingobacterium canadensis SC202103 microbial inoculum in the degradation of alpha-solanine.

Test example 1

Isolation and characterization of Sphingobacterium canadensis SC202103

The present inventors isolated sphingosine canadensis SC202103 strain from the midgut of potato tuber moth larvae at 3 months and 25 days 2021. The inventor finds that the strain has a strong degradation effect on alpha-solanine.

The following is a test example of sphingosine canadensis SC202103 and its application in degradation of alpha-solanine provided by the present invention.

1.1 materials

1.1.1 test strains

The strain was isolated from the midgut of Spodoptera frugiperda larvae at 3 months and 25 days of 2021, Kunming, Yunnan university of Yunnan agriculture, Kunming, Yunnan province.

1.1.2 isolation and culture of the Strain

Stripping 50 healthy tuber moth 3-instar larvae, repeating for 3 times, totally 150 larvae, and starving in a sterile culture dish for 8 h. Dissecting in a clean bench, cleaning the bench surface of the clean bench, sterilizing all dissecting instruments with 75% alcohol, and irradiating with ultraviolet lamp for more than 30 min. Freezing the larvae of potato tuber moth at-20 deg.C for 2-3min before dissection, washing with sterile water for 2 times (1 min each time) after paralysis, sterilizing with 75% alcohol for 90s, and washing with sterile water for 2 times (1 min each time). Taking out and placing in a sterile clean culture dish, taking out the intestinal tract from the tail of the polypide by using a sterile forceps, dissolving the content in a centrifuge tube filled with 1mL of sterile PBS buffer solution, and fully homogenizing. The purified strain was stored in a 4 ℃ freezer using SDAY slant medium.

1.1.3 colony morphology Observation

And selecting the single bacterial colony of the high-efficiency degradation bacterial strain obtained by screening, streaking the single bacterial colony on an LB culture medium, culturing for 72h at 25 ℃, and observing and recording the characteristics of the single bacterial colony, such as shape, color, swelling degree, edge shape, dryness, wetness and the like according to the identification methods of a conventional bacteria system identification manual and a Bergey bacteria identification manual. And staining the strain by a gram staining method, observing the cell morphology and the cell color, wherein purple represents positive bacteria, and red represents negative bacteria.

And (3) molecular identification: bacterial DNA (Von Guangda et al, 2013; Zhenghai et al, 2017) was extracted by a freeze-thaw method, specifically as follows: the strains are respectively inoculated in LB liquid culture medium, then placed on a shaking table at 25 ℃ and 180r/min, and shake-cultured for 48 h. 2mL of bacterial liquid of each strain is respectively taken and put in a centrifuge tube, centrifuged for 2min at 12000r/min, and the supernatant is discarded. Adding 500 μ L sterile water into the precipitate, and vortex shaking for 1min to completely suspend the thallus. Then freezing with liquid nitrogen for 10min, carrying out boiling water bath for 5min, centrifuging at 12000r/min for 2min, and taking the supernatant as PCR template DNA for later use. 16S rRNA gene was amplified using bacterial universal primers 27f and 1492 r. The reaction system is 25 μ L: 1. mu.L of each primer, 1. mu.L of template DNA,

taq PCR master mix 12.5. mu.L, dd H2O 9.5.5. mu.L. The reaction condition is that the thermal denaturation is carried out for 5min at 94 ℃; denaturation at 94 ℃ for 1min, annealing at 53 ℃ for 1min, extension at 72 ℃ for 2min, 32 cycles, and extension at 72 ℃ for 10 min. Taking 3 mu L of PCR product to carry out gel electrophoresis detection. The identified PCR products were sent to the company Biotechnology engineering (Kunming) Ltd for sequencing. And (3) splicing the sequencing result by using contigxxpress software, comparing the spliced 16S rDNA sequence with data in an NCBI database through an NCBI Blast program, searching a typical strain with the highest similarity and downloading a sequence (as follows). And (3) constructing a phylogenetic tree by using MEGA7 software and adopting an international universal adjacency method (Neighbor-Joining) and a Kimura two-parameter correction model. Bootstrap verification is carried out for 1000 times of repeated sampling, and the stability of the topological structure of the phylogenetic tree is analyzed and evaluated.

16S sequence number of a sphingosine canadensis strain:

GCTATACATGCAAGTCGGACGGGATCCGTTGGTAGCTTGCTATCAATGGTGAGAGTGGCGCACGGGTGCGTAACGCGTGAGCAACCTACCTCTATCAGGGGGATAGCCTCTCGAAAGAGAGATTAAGACCGCATAATATAATCTACCGGCATCGGGAGATTATTAAATATTTATAGGATAGAGATGGGCTCGCGTGACATTAGCTAGTTGGTAGGGTAACGGCTTACCAAGGCGACGATGTCTAGGGGCTCTGAGAGGAGAATCCCCCACACTGGTACTGAGACACGGACCAGACTCCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGCAGGATGACTGCCCTATGGGTTGTAAACTGCTTTTGTCCAGGAATAAACCTAAATACGTGTATTTAGCTGAATGTACTGGAAGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTATTAAGTCAGGGGTGAAATACGGTGGCTCAACCATCGCAGTGCCTTTGATACTGATGGGCTTGAATCCATTTGAAGTGGGCGGAATAAGACAAGTAGCGGTGAAATGCATAGATATGTCTTAGAACTCCGATTGCGAAGGCAGCTCACTAAGCTGGTATTGACGCTGATGCACGAAAGCGTGGGGATCGAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGATAACTCGATGTTGGCGATAGACAGCCAGCGTCTTAGCGAAAGCGTTAAGTTATCCACCTGGGGAGTACGCCCGCAAGGGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGAGGAGCATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCCGGGCTTGAAAGTTAGTGAAGGATGCAGAGACGCATCCGTCCTTCGGGACACGAAACTAGGTGCTGCATGGCTGTCGTCAGCTCGTGCCGTGAGGTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATGTTTAGTTGCCAGCATTTAAGGTGGGGACTCTAAACAGACTGCCTGTGCAAACAGTGAGGAAGGTGGGGACGACGTCAAGTCATCATGGCCCTTACGTCCGGGGCTACACACGTGCTACAATGGATGGTACAGCGGGCAGCTACATAGCAATATGGTGCTAATCTCTAAAAGCCATTCACAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTTGGATTCGCTAGTAATCGCGTATCAGCAATGACGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGAAAGTTGGGGGTACCTAAAGCATGTGACCGCAAGGAGCGTGTAGGTAAAGCCCCT。

1.2 results

The bacterial colony of the strain S1 is round, neat in edge, orange yellow, smooth and moist in surface, slightly convex in center and flat in periphery after being subjected to LB plate streak purification culture. The cells were stained red by gram staining, gram-negative bacteria, and the cells were short rod-shaped (FIG. 1).

0-2h is a retardation phase, 2-10h is a logarithmic growth phase, wherein the growth is fastest in 6-8 h; the growth period is 10-28h, and the growth rate slowly decreases after 28h reaches the maximum growth rate; 34-48h are decay periods (FIG. 2 is the growth curve of Sphingobacterium canadensis SC202103 of the present invention).

Test example 2

Determination of the degradation of alpha-solanine by Sphingobacterium canadensis SC202103

1 Material

The sphingosine canadensis SC202103 strain was used. Selecting 5mL of Sphingobacterium canadensis monoclonal colony, inoculating into LB liquid culture medium, and shake culturing at 25 deg.C and 180rpm to make OD_600nm＝1.0。

Induction: using a 100 mL-volume Erlenmeyer flask, 1mL of the bacterial culture was added to 9mL of LB medium (final OD)_600nm0.1), supplemented with acidic phosphate buffer (control) or 5 μ g/mL α -solanine, and shake-cultured at 180rpm and 25 ℃ for 20 h. Centrifuging the bacterial liquid at 25 deg.C at 4000 Xg for 20min to obtain supernatant as the crude extracellular enzyme extract of Sphingobacterium canadensis. Repeat 3 times.

Extracellular enzyme degradation system: mu.L of crude extracellular extract was prepared by incubating 100. mu. LpH 5.5.5 in 50mM sodium acetate buffer, 400. mu. LpH 5.0100mM sodium acetate buffer (0.2mM alpha-solanine) as substrate, and 450. mu.L of crude extracellular extract for 35h at 25 ℃. The enzyme reaction was stopped by heating at 90 ℃ for 10min, followed by centrifugation at 17000 Xg for 10min and transfer of the supernatant. The intermediate metabolites produced during degradation were analyzed by LC-MS.

2. Results

The alpha-solanine degradation capability is measured by adopting the extracellular enzyme of the high-efficiency degradation strain, and the result shows that the degradation rate of the Sphingobacterium canadensis SC202103 strain reaches 99.79%. The degradation of α -solanine by the strain sphingosine canadensis SC202103 is shown in table 1:

TABLE 1 degradation of alpha-solanine by primary strains

According to the determination of the degradation capability of the extracellular enzyme of the Sphingobacterium canadensis SC202103 strain on the alpha-solanine, the degradation rate of the extracellular enzyme of the Sphingobacterium canadensis SC202103 strain on the alpha-solanine is up to 99.79 percent. Thus, the strain sphingosine canadensis SC202103 has strong degradation ability to alpha-solanine.

In conclusion, the sphingosine canadensis SC202103 strain is an insect intestinal symbiotic bacterium capable of degrading alpha-solanine, has the characteristics of strong degradation effect and no environmental pollution, and can be widely used for biodegradation of alpha-solanine.

While the foregoing description shows and describes several preferred embodiments of this invention, it is to be understood, as noted above, that this invention is not limited to the forms disclosed herein, but is not intended to be exhaustive or to exclude other embodiments and may be used in various other combinations, modifications, and variations within the scope of the inventive concept, as may be realized by the teachings set forth above or as may be learned by the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.

Sequence listing

<110> Yunnan university of agriculture

<120> sphingosine canadensis SC202103 strain for degrading alpha-solanine and application thereof

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<213> Artificial sequence (16S rDNA Gene sequence of Sphingobacterium canadensis SC202103 Strain)

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gctatacatg caagtcggac gggatccgtt ggtagcttgc tatcaatggt gagagtggcg 60

cacgggtgcg taacgcgtga gcaacctacc tctatcaggg ggatagcctc tcgaaagaga 120

gattaagacc gcataatata atctaccggc atcgggagat tattaaatat ttataggata 180

gagatgggct cgcgtgacat tagctagttg gtagggtaac ggcttaccaa ggcgacgatg 240

tctaggggct ctgagaggag aatcccccac actggtactg agacacggac cagactccta 300

cgggaggcag cagtaaggaa tattggtcaa tgggcggaag cctgaaccag ccatgccgcg 360

tgcaggatga ctgccctatg ggttgtaaac tgcttttgtc caggaataaa cctaaatacg 420

tgtatttagc tgaatgtact ggaagaataa ggatcggcta actccgtgcc agcagccgcg 480

gtaatacgga ggatccgagc gttatccgga tttattgggt ttaaagggtg cgtaggcggc 540

ctattaagtc aggggtgaaa tacggtggct caaccatcgc agtgcctttg atactgatgg 600

gcttgaatcc atttgaagtg ggcggaataa gacaagtagc ggtgaaatgc atagatatgt 660

cttagaactc cgattgcgaa ggcagctcac taagctggta ttgacgctga tgcacgaaag 720

cgtggggatc gaacaggatt agataccctg gtagtccacg ccctaaacga tgataactcg 780

atgttggcga tagacagcca gcgtcttagc gaaagcgtta agttatccac ctggggagta 840

cgcccgcaag ggtgaaactc aaaggaattg acgggggccc gcacaagcgg aggagcatgt 900

ggtttaattc gatgatacgc gaggaacctt acccgggctt gaaagttagt gaaggatgca 960

gagacgcatc cgtccttcgg gacacgaaac taggtgctgc atggctgtcg tcagctcgtg 1020

ccgtgaggtg ttgggttaag tcccgcaacg agcgcaaccc ctatgtttag ttgccagcat 1080

ttaaggtggg gactctaaac agactgcctg tgcaaacagt gaggaaggtg gggacgacgt 1140

caagtcatca tggcccttac gtccggggct acacacgtgc tacaatggat ggtacagcgg 1200

gcagctacat agcaatatgg tgctaatctc taaaagccat tcacagttcg gattggggtc 1260

tgcaactcga ccccatgaag ttggattcgc tagtaatcgc gtatcagcaa tgacgcggtg 1320

aatacgttcc cgggccttgt acacaccgcc cgtcaagcca tgaaagttgg gggtacctaa 1380

agcatgtgac cgcaaggagc gtgtaggtaa agcccct 1417

Claims

1. A sphingosine canadensis Sphingobacterium sphingomonas canadensis strain SC202103 for degrading alpha-solanine, characterized in that: the strain is sphingosine bacteria Sphingobacterium canadensis with a preservation name of sphingosine bacteria Sphingobacterium canadensis; is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation address is No. 3 of Xilu No.1 of Beijing, Chaoyang district; the preservation date is as follows: 31/5/2021; the preservation number is: CGMCC No. NO22640.

2. The α -solanine degrading sphingosine canadensis SC202103 strain according to claim 1 characterized in that: the 16S rDNA gene sequence of the Sphingobacterium canadensis SC202103 strain is the nucleotide sequence shown in SEQ ID No. 1.

3. The α -solanine degrading sphingosine canadensis SC202103 strain according to claim 1 characterized in that: the bacterial strain S1 is purple after gram staining, is a gram-positive bacterium, has a rod-shaped cell morphology, and is found to be round, neat in edge, orange-yellow, smooth and moist in surface, slightly convex in center and flat in periphery after being subjected to LB plate streak purification culture. The cells are red by gram staining, gram-negative bacteria and short rod-shaped.

4. The sphingosine canadensis SC202103 strain preparation for degrading α -solanine according to claim 1, sphingosine canadensis SC202103 strain preparation.

5. The bacterial agent of the sphingosine canadensis SC202103 strain according to claim 3, wherein the active ingredient is at least one of the following (a), (b), (c):

(a) a fermentation broth first extract of the sphingosine canadian SC202103 strain of claim 1;

(b) ultrasonically lysed supernatant of cells of the sphingosine canadensis SC202103 strain obtained according to claim 1;

(c) ultrasonic lysis of cells of the Sphingobacterium canadensis SC202103 strain obtained according to claim 1.

6. The process for producing the bacterial agent of sphingosine canadensis SC202103 strain according to any of claims 1 to 5, characterized by comprising the steps of:

(1) separating Sphingobacterium canadensis SC202103 from the midgut of the potato tuber moth larva, and culturing by adopting LB culture medium;

(2) then purifying on an LB culture medium to obtain purified sphingosine canadensis SC 202103;

(3) the alpha-solanine degradation capacity is measured by adopting high-efficiency degradation strain bacterial liquid, and the degradation rate of the bacterial liquid to the alpha-solanine reaches 99.79 percent.

7. Use of the sphingosine canadensis SC202103 strain according to claim 1 in a preparation for the degradation of alpha-solanine.