CN114181871A - Brevibacillus laterosporus strain and application thereof - Google Patents
Brevibacillus laterosporus strain and application thereof Download PDFInfo
- Publication number
- CN114181871A CN114181871A CN202111680045.4A CN202111680045A CN114181871A CN 114181871 A CN114181871 A CN 114181871A CN 202111680045 A CN202111680045 A CN 202111680045A CN 114181871 A CN114181871 A CN 114181871A
- Authority
- CN
- China
- Prior art keywords
- brevibacillus laterosporus
- strain
- soil
- cfu
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Tropical Medicine & Parasitology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a Brevibacillus laterosporus strain and application thereof, and relates to a microorganism application technology, wherein the preservation number of the strain is CGMCC No.22964, and the fermentation product of the strain is remarkable in resisting botrytis cinerea and degrading carbamate pesticide pollution.
Description
Technical Field
The invention relates to a microorganism application technology, in particular to a strain containing specific Brevibacillus laterosporus and application thereof.
Background
Brevibacillus laterosporus (Brevibacillus laterosporus) is an aerobic bacterium distributed in cold, warm and hot zones and exists on the surface of plants, in the bodies of insects, in soil, fresh water or seawater. Various metabolites, such as sporotrichum amine, peptide antibiotics, polyketides, chitinase and the like at different levels can be generated in the growth process of the brevibacillus laterosporus, and some excellent strains are prepared into bacteriostat, immunomodulator, soil conditioner and the like according to the characteristics and are widely applied to the fields of medicine, agriculture and breeding industry.
The quality and the efficacy of the brevibacillus laterosporus preparation product mainly depend on the characteristics of the strains, and the screening of good strains is a precondition for providing high-quality products. The strain may be degenerated, mutated and the like in the process of passage; therefore, there is a need to continuously develop and screen excellent strains to provide candidate materials for brevibacillus laterosporus preparation products.
Disclosure of Invention
In order to enrich available products in the field and provide a high-quality agricultural biocontrol microbial inoculum, the invention develops and screens a multifunctional brevibacillus laterosporus strain, which comprises the following specific steps:
a Brevibacillus laterosporus strain CB202103-8 with the preservation number of CGMCC No.22964.
A fermentation broth comprising the above Brevibacillus laterosporus.
The application of the brevibacillus laterosporus in resisting tomato gray mold is characterized by comprising any one of the following measures
(1) Spraying the fermentation liquor to tomato plants at any stage of the tomato growth and development period;
(2) spraying the fermentation liquor on the picked tomatoes;
(3) spraying the fermentation liquor on the soil of the tomato cultivation land;
the effective concentration of the Brevibacillus laterosporus in the fermentation liquor is 1 multiplied by 105～1×107CFU/mL; or 5X 105～ 5×106CFU/mL.
The application of the brevibacillus laterosporus in preparing a soil conditioner.
The application of the brevibacillus laterosporus in treating the organic pesticide pollution of soil is characterized in that fermentation liquor of the brevibacillus laterosporus is sprayed to farmlands to be treated.
The method comprises the steps of screening pathogenic bacteria of tomato gray mold and testing carbamate degradation of 150 strains separated from a farmland environment, selecting a strain of Brevibacillus laterosporus, and testing to determine that the Brevibacillus laterosporus can resist the pathogenic bacteria of tomato gray mold and greatly degrade carbamate pesticides contained in soil. The microbial inoculum is beneficial to preventing and treating the botrytis cinerea, improving the yield and the tomato quality and reducing the loss; therefore, the isolated strain is sent to the China general microbiological culture Collection center for preservation, and the following technical scheme is claimed according to the request:
preservation information:
the preservation unit: china general microbiological culture Collection center
And (4) storage address: xilu No.1 Hospital No.3, 100101, of Chaozhou, Chaoyang, region
The preservation date is as follows: 7/27/2021
The strain name is: CB202103-8
The preservation number is as follows: CGMCC No.22964.
And (3) classification and naming: brevibacillus laterosporus of Brevibacillus laterosporus
Detailed Description
The present invention will be described below with reference to specific examples.
1. Isolation of the Strain
Sampling time: 3 months in 2021
Sampling site: zhengzhou Henan
Sampling 150 parts of farmland soil, 5-10g of each part, putting each part of sample into a triangular flask, adding 200ml of sterilized water, stirring uniformly, shaking for 10 minutes to prepare a suspension, taking 1ml of the suspension, adding 9ml of double distilled water, and then performing gradient dilution by using the double distilled water; coating 200ul of diluent on an LB plate culture medium, culturing for 20-50 hours at 25-35 ℃, picking the dominant single colony with consistent shape, transferring to the LB plate culture medium, streaking and purifying, taking part for analysis, detection and screening, and storing the rest in a refrigerator at-70 ℃.
2. Strain screening and identification
2.1 screening antagonistic bacteria against tomato gray mold pathogenic bacteria
Botrytis cinerea (Botrytis cinerea) slant strain, CGMCC No.3.4583, purchased from China general microbiological culture Collection center.
Culture medium: PDA culture medium: 200g of potato, 20g of glucose, 15-20g of agar, 1000 mL of tap water and natural pH; PDB liquid medium: agar is not added, and other components are the same as PDA culture medium.
Inoculating the pathogenic bacteria on PDA culture medium, and culturing at 22 deg.C in inverted manner.
Adopting a flat plate opposing method. Placing activated pathogenic bacteria cake (diameter 6mm) in the center of PDA plate with puncher, and culturing at 22 deg.C for 24 hr; picking purified strain to be tested by using sterilized toothpick, inoculating the strain to 4 angular points at 3cm from the center of the plate, and culturing at 30 ℃ for 3-5 days. Setting 3 times of treatment, using a plate only inoculated with pathogenic bacteria as a reference, observing the growth state of the pathogenic bacteria, selecting strains which have inhibition effect on the growth of the pathogenic bacteria, are parallel to the edges of inhibited pathogenic bacteria and have lasting antagonism, measuring the colony diameter of the pathogenic bacteria, and calculating the inhibition rate of the strains:
the inhibition rate (%) was [ (control colony diameter-treatment colony diameter)/(control colony diameter-cake diameter) ] x 100%.
20 candidate strains are selected in the experiment, the inhibition rate is 65.05-80.31%, and the inhibition rate of CB202103-8 claimed by the invention reaches 78.85%.
2.2 morphological characterization of antagonistic bacteria
And performing morphological identification on the selected candidate antagonistic strain.
The CB202103-8 claimed by the invention has smooth colony edges, opaque gray white color and no folds on the surface on an LB culture medium. Observation under an electron microscope: the bacterial body is rod-shaped, periphytic flagellum, and the colony size is about (0.6-1) μm x (2.0-4.0) μm, and spore outgrowth is observed.
Variable gram staining results: delay period G-Log phase G+Stationary phase G-。
Identifying with Phoenix 100 type full-automatic microorganism analyzer, and detecting selected strain CB202103-8 by referring to the strain physiological and biochemical detection method of Bergey bacteria identification handbook:
the strain CB202103-8 can normally grow at the temperature of 10-40 ℃ and the pH value of 7-9, the optimal growth temperature is 20-30 ℃, and the pH value is 7.2-8.0. The primary identification is determined to be Brevibacillus laterosporus.
2.316S rDNA sequence analysis and identification
16S rDNA sequence amplification specific primers were synthesized for the commitment as follows:
the forward Primer A5'-AGAGTTTGATCCTGGCTCAG-3' (Seq ID No.1),
reverse Primer B: 5'-TACGGATACCTTGTTACGACTT-3' (Seq ID No. 2).
Using TaKaRaTMExtracting the genome DNA of the candidate strain by using the bacterial genome DNA extraction kit, and performing PCR amplification by using the extracted genome DNA as a template.
PCR reaction (50. mu.L): TaKaRa LA-Taq 0.5 μ L, DNA template 2 μ L, 10 XPCR Buffer 5 μ L, dNTP mix 8 μ L, upstream and downstream primers 2 μ L each, double distilled water 30.5 μ L.
PCR procedure: pre-denaturation at 94 ℃ for 5min, at 94 ℃ for 30s, at 54 ℃ for 1min, at 72 ℃ for 2min, and 30 cycles; extension at 72 ℃ for 7 min.
And (4) sequencing the PCR product after purification, and performing Blast comparison analysis on the obtained sequencing result through an NCBI database.
The results show that: the size of the 16S rDNA fragment of the selected strain CB202103-8 is 1526bp, and the similarity is 99 percent when the fragment is compared with the known Brevibacillus laterosporus.
The evolutionary relationship is analyzed by MEGA5.0 software, and the result shows that CB202103-8 has the closest genetic relationship with Brevibacillus laterosporus and is determined to be Brevibacillus laterosporus.
The separated strain is sent to the China general microbiological culture Collection center for preservation, and the strain name is as follows: CB202103-8 with the deposition number: CGMCC No.22964.
3. Strain detection
3.1 Brevibacillus laterosporus CB202103-8 detection of tomato Gray mold prevention
The preserved strain CB202103-8 is activated on an LB culture medium for 24 hours, and a single colony is picked and inoculated in 20mL of an LB liquid culture medium to be a strain liquid. Inoculating the mixture to 100ml of culture medium according to the proportion of 1 percent, culturing at 37 ℃, and carrying out constant temperature shaking culture at 200r/minAfter 24 hours, a CB202103-8 culture solution was obtained. Diluted to 1x10 with sterile water7CFU/mL。
Perforating the edge of Botrytis cinerea growing on PAD culture medium for 5 days by using a perforator, inoculating a bacterium block into 50mL of sterile water, performing shaking culture at 28 ℃ and 180rpm for 10 minutes, and diluting to 1x106CFU/mL。
Sterilizing the surface of the tomato fruit: the swab was wiped with 75% alcohol for 30s and washed 3 times with sterile water. Punching with a sterilizing gun head to form 6 holes on the surface of each fruit; each treated 10 fruits. The concentrations of the culture solutions of the treatment groups CB202103-8 were 1X10, respectively7、 5×106、1×106、5×105And 1X105CFU/mL, 20. mu.L of each culture medium was added to each well, and after 2 hours, 20. mu.L of 1X10 was added6The CFU/mL botrytis cinerea suspension is inoculated with botrytis cinerea only as a positive control CK1, and sterile water as a negative control CK 2. Measuring the prevention and treatment effect of antagonistic bacteria CB202103-8 on tomato gray mold (Botrytis cinerea), keeping moisture in the dark for 48 h, culturing at 28 deg.C, observing the morbidity, and calculating the morbidity and disease index.
Disease severity grading criteria were as follows: grade 0 is disease-free; 1 grade 1 disease hole; 2 grade 2 disease holes; 3 grade 3 disease holes; 4 grade 4 disease holes; 5 grades, 5-6 disease holes.
Disease index (%) { Σ [ (number of diseased fruits at each stage × relative stage value) ]/total number of investigated fruits × 5} × 100%
The control effect (%) is { [ positive control disease index-treatment disease index ]/positive control disease index } × 100%
The results of the investigation are shown in table 1 below:
TABLE 1 prevention of tomato gray mold (Botrytis cinerea) by CB202103-8
As can be seen from Table 1, the tomatoes added with the high-concentration antagonistic bacteria CB202103-8 have no disease on day 3, and the disease reaches 100%, 88.2% on day 5 and 80.5% on day 7; when the concentration of the antagonistic bacteria is reduced to 5 multiplied by 106At the time of CFU/mL,the control effect and the high concentration have no significant difference on the 3 rd day and the 5 th day, but the control effect is obviously reduced on the 7 th day, which shows that the control effect and the persistence of the low concentration are reduced; when the concentration of the antagonistic bacteria A60 is lower than 5X 105At CFU/mL, although some reduction, still has significant control effect. As the biocontrol microbial inoculum, the concentration is recommended to be 1 multiplied by 105～1×107CFU/mL, preferably, 5X 105～ 5×106CFU/mL.
3.2 testing the adsorption and degradation functions of the soil carbamate pesticides
In the prior art, Brevibacillus laterosporus probably has degradation effect on soil organic pollutants. The carbamate compounds are widely used as insecticides, acaricides, herbicides [ benazolin, N- (3, 4-dichlorophenyl) methyl carbamate) ] and bactericides in pesticides, so that the carbamate pesticides are accumulated in large-area farmlands to cause pesticide residues in vegetables and fruits, and the carbamate compounds are key detection varieties of the pesticide residues in vegetables and fruits.
Therefore, the invention performs carbamate pesticide degradation tests on 20 candidate antagonistic strains (containing CB202103-8) selected from 2.1, and hopes to select dominant strains with good tomato gray mold resistance and organic pollutant degradation function.
Reagent:
mefenamate (CAS #:1918-18-9), 0.1Mg/ML, Beijing Bailingwei science and technology Limited
Experiment design:
experimental group 1. in a 250mL triangular flask with a stopper, 100g of a soil sample was added, and 5X 10 of an antagonistic strain LB medium culture solution was added65mL of CFU/mL, and then 100mL of benazolin (the concentration is 100ug/Kg) diluted by sterile water; then placing the mixture in a water bath constant temperature oscillator at 25 ℃ for culturing, sampling at different time points, centrifuging at the rotating speed of 4,000 g for 20 minutes, and taking supernatant to determine the concentration of the benazolin;
experimental group 2. in a 250mL triangular flask with a stopper, 5X 10 of antagonistic strain LB medium culture solution was added6CFU/mL 5mL, then adding sterile water diluted benazolin 100mL (concentration of 100 ug/L); then placing the mixture in a water bath constant temperature oscillator at 28 ℃ for culturingSampling at different time points, centrifuging at the rotating speed of 4,000 g for 20 minutes, and taking supernatant to determine the concentration of the benazolin;
control group: adding 100g of soil sample into a 250mL triangular flask with a plug, and adding 100mL of benazolin (the concentration is 100ug/Kg) diluted by sterile water; then placing the mixture in a water bath constant temperature oscillator at 25 ℃ for culturing, sampling at different time points, centrifuging at the rotating speed of 4,000 g for 20 minutes, and taking supernatant to determine the concentration of the benazolin;
the concentration of the benazolin is completely determined according to the standard operation flow of GB5085.6-2007 appendix H. The measurement results are shown in Table 2.
TABLE 2 dynamic variation of concentration of imazapyr in CB202103-8 treatment
The measurement results showed that the experimental groups 1, 2 and the control group reached equilibrium after 24 hours; in 12 hours, the concentration of the benazolin in the experimental group 1 is reduced by 44% compared with the initial concentration, and when the concentration reaches the balance, the concentration is reduced by more than 65%; the results of the experimental group 2 without soil were close to those of the experimental group 1. The decrease of the concentration of the benazolin is illustrated, and the benazolin is mainly degraded by biological utilization of added antagonistic bacteria (CB202103-8) instead of soil adsorption; the concentration of the benazolin in the control group also decreased with time due to its natural volatilization and degradation; in general, the strain CB202103-8 shows obvious effects on degrading carbamate pesticides represented by imazalil and is an excellent candidate strain serving as a soil pollution improver.
Claims (6)
1. A Brevibacillus laterosporus strain CB202103-8 with the preservation number of CGMCC No.22964.
2. A fermentation broth comprising Brevibacillus laterosporus of claim 1.
3. Use of Brevibacillus laterosporus as claimed in claim 1 for the resistance of tomato gray mold, comprising any of the following measures
(1) Spraying the fermentation broth of claim 2 onto tomato plants at any stage of their growth and development;
(2) spraying the fermentation broth of claim 2 onto the harvested tomatoes;
(3) spraying the fermentation broth of claim 2 on the soil of a tomato cultivation plot;
the effective concentration of the Brevibacillus laterosporus in the fermentation liquor is 1 multiplied by 105～1×107CFU/mL; or 5X 105～5×106CFU/mL.
4. Use of Brevibacillus laterosporus according to claim 1 for the preparation of a soil conditioner.
5. The use of Brevibacillus laterosporus in the treatment of organic pesticide pollution in soil as claimed in claim 1, wherein the fermentation liquid of Brevibacillus laterosporus is sprayed to the farmland to be treated.
6. The use according to claim 6, wherein the organic pesticide is a carbamate compound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111680045.4A CN114181871B (en) | 2021-12-31 | 2021-12-31 | Brevibacillus laterosporus strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111680045.4A CN114181871B (en) | 2021-12-31 | 2021-12-31 | Brevibacillus laterosporus strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114181871A true CN114181871A (en) | 2022-03-15 |
| CN114181871B CN114181871B (en) | 2023-08-15 |
Family
ID=80606618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111680045.4A Active CN114181871B (en) | 2021-12-31 | 2021-12-31 | Brevibacillus laterosporus strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114181871B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480046A (en) * | 2014-12-18 | 2015-04-01 | 江苏省农业科学院 | Brevibacillus laterosporus strain and application thereof |
| WO2015167809A1 (en) * | 2014-04-28 | 2015-11-05 | Syngenta Participations Ag | Method for controlling nematode pests |
| CN107151641A (en) * | 2017-07-04 | 2017-09-12 | 沈阳农业大学 | One plant suppression Rhizoctonia solani Kuhn Brevibacillus laterosporus and its application |
| CN111269865A (en) * | 2020-04-01 | 2020-06-12 | 北京工商大学 | Brevibacillus laterosporus strain S62-9 and application thereof |
| CN111394272A (en) * | 2019-12-30 | 2020-07-10 | 武汉合缘绿色生物股份有限公司 | Brevibacillus laterosporus and application thereof |
| CN111748496A (en) * | 2020-07-03 | 2020-10-09 | 天津坤禾生物科技集团股份有限公司 | Application of Brevibacillus laterosporus MES818 in tomato cultivation |
-
2021
- 2021-12-31 CN CN202111680045.4A patent/CN114181871B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015167809A1 (en) * | 2014-04-28 | 2015-11-05 | Syngenta Participations Ag | Method for controlling nematode pests |
| CN104480046A (en) * | 2014-12-18 | 2015-04-01 | 江苏省农业科学院 | Brevibacillus laterosporus strain and application thereof |
| CN107151641A (en) * | 2017-07-04 | 2017-09-12 | 沈阳农业大学 | One plant suppression Rhizoctonia solani Kuhn Brevibacillus laterosporus and its application |
| CN111394272A (en) * | 2019-12-30 | 2020-07-10 | 武汉合缘绿色生物股份有限公司 | Brevibacillus laterosporus and application thereof |
| CN111269865A (en) * | 2020-04-01 | 2020-06-12 | 北京工商大学 | Brevibacillus laterosporus strain S62-9 and application thereof |
| CN111748496A (en) * | 2020-07-03 | 2020-10-09 | 天津坤禾生物科技集团股份有限公司 | Application of Brevibacillus laterosporus MES818 in tomato cultivation |
Non-Patent Citations (2)
| Title |
|---|
| JATOI GH 等: "A Novel Protein Elicitor PeBL2, from Brevibacillus laterosporus A60, Induces Systemic Resistance against Botrytis cinerea in Tobacco Plant", 《PLANT PATHOL J》, vol. 35, no. 3, pages 208 - 218 * |
| 苗素平: "不同浓度侧孢短芽孢杆菌对土壤与作物生长的影响", 《济宁学院学报》, vol. 37, no. 6, pages 38 - 42 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114181871B (en) | 2023-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Beneduzi et al. | Diversity and plant growth promoting evaluation abilities of bacteria isolated from sugarcane cultivated in the South of Brazil | |
| Shimizu et al. | A promising strain of endophytic Streptomyces sp. for biological control of cucumber anthracnose | |
| Agaras et al. | Quantification of the potential biocontrol and direct plant growth promotion abilities based on multiple biological traits distinguish different groups of Pseudomonas spp. isolates | |
| Ilyas et al. | Azospirillum strains isolated from roots and rhizosphere soil of wheat (Triticum aestivum L.) grown under different soil moisture conditions | |
| CN111154688B (en) | Biocontrol bacillus beleisi SF259 and application thereof | |
| Navarro-Ródenas et al. | Beneficial native bacteria improve survival and mycorrhization of desert truffle mycorrhizal plants in nursery conditions | |
| CN107338202B (en) | Bacillus amyloliquefaciens with broad-spectrum pathogenic bacterium inhibiting function and application thereof | |
| CA2822884A1 (en) | Fertilizer composition and method | |
| CN111040976A (en) | Bacillus amyloliquefaciens and application thereof | |
| CN107794239B (en) | Bacillus toyoyo strain, preparation method and application of microbial inoculum | |
| Assigbetse et al. | Soil bacterial diversity responses to root colonization by an ectomycorrhizal fungus are not root-growth-dependent | |
| CN107699526B (en) | Actinomycete strain for preventing and treating gray mold and application thereof | |
| Jafari et al. | Exploration, antifungal and antiaflatoxigenic activity of halophilic bacteria communities from saline soils of Howze-Soltan playa in Iran | |
| CN109182216B (en) | Marine streptomyces SCFJ-05 with inhibition effect on succulent plant stem rot | |
| Oskay | Comparison of Streptomyces diversity between agricultural and non-agricultural soils by using various culture media | |
| CN114181871B (en) | Brevibacillus laterosporus strain and application thereof | |
| CN110904005B (en) | Fomesafen degrading bacterium and application thereof | |
| CN113373091A (en) | Biocontrol strain bacillus thuringiensis FJ2B-25 for preventing and treating rice sheath blight | |
| Hassanien et al. | Multifaceted potentialities of some rhizobacteria associated with sorghum plants on their growth and development | |
| Nehra et al. | Potential plant growth promoting activity of Pseudomonas fluorescens sp. isolated from cotton (Gossypium hirsutum) crop | |
| CN115627247B (en) | Preparation method and application of compound microbial fertilizer | |
| CN116042458B (en) | Streptomyces nucleolus SM3-7 and application thereof in prevention and treatment of corn stem basal rot | |
| Lebrazi et al. | Phenotypic, molecular, and symbiotic characterization of the rhizobial symbionts isolated from Acacia saligna grown in different regions in Morocco: a multivariate approach | |
| Al-Healy et al. | Effects of streptomyces rochi Biosurfactants on Pathogenic Staphylococcus aureus and Pseudomonas aeruginosa | |
| CN113832074B (en) | Actinomycetes, and biocontrol microbial inoculum and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ma Lintao Inventor after: Wang Li Inventor after: Wang Fuyong Inventor after: Zhang Heng Inventor after: Tian Xiao Inventor before: Ma Lintao Inventor before: Wang Li |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |