CN114166906A - Multi-project detection all-in-one machine - Google Patents
Multi-project detection all-in-one machine Download PDFInfo
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- CN114166906A CN114166906A CN202111472381.XA CN202111472381A CN114166906A CN 114166906 A CN114166906 A CN 114166906A CN 202111472381 A CN202111472381 A CN 202111472381A CN 114166906 A CN114166906 A CN 114166906A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8822—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving blood
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Abstract
The invention discloses a multi-item detection all-in-one machine, which comprises an electrochemical detection mechanism, a high-pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used for detecting the contents of blood sugar and fructosamine, and the high-pressure liquid chromatography mechanism is used for detecting the content of glycosylated hemoglobin; the sample adding mechanism is connected with the high-pressure liquid chromatography mechanism and used for conveying a sample solution to the high-pressure liquid chromatography mechanism and dropping the sample solution into the electrochemical detection mechanism. According to the invention, the electrochemical detection mechanism and the high-pressure liquid chromatography mechanism are integrated together, so that the multi-item detection integrated machine can simultaneously detect blood sugar, fructosamine and glycosylated hemoglobin, thereby avoiding the need of performing three detection items on three different instrument platforms, improving the detection efficiency, and simultaneously avoiding the need of performing multiple blood drawing operations on patients when detecting different items.
Description
Technical Field
The invention relates to the technical field of biological index detection, in particular to a multi-item detection all-in-one machine.
Background
At present, the prevalence rate and incidence rate of diabetes rapidly rise in the world, and the means for detecting diabetes are more and have advantages and disadvantages. Generally speaking, blood sugar is the most important experimental test item in the process of diabetes diagnosis and treatment, and reflects the instantaneous level of glucose in a patient, but the blood sugar is easily influenced by a plurality of factors such as diet, medicines, emotion and the like, and sometimes cannot objectively reflect the long-term level of glucose in a human body. Fructosamine reflects the average level of blood sugar of a patient 2-3 weeks before measurement, is sensitive to blood sugar change ratio HbA1c in a short period, is a good index for evaluating the short-term glucose metabolism control condition of the patient, especially for evaluating the curative effect of the diabetic after adjustment of a treatment scheme, but the specificity of some detection methods of fructosamine is not high, and the interference of urate and hyperlipidemia exists. Glycated hemoglobin (HbA1c) is a product of hemoglobin and glucose in red blood cells, and is formed by slow, continuous and irreversible glycation, and the content of glycated hemoglobin (HbA1c) depends on blood glucose concentration and the contact time of blood glucose and hemoglobin, and is independent of factors such as blood drawing time, fasting state of a patient, insulin use, etc., and HbA1c can effectively reflect the blood glucose control of a diabetic patient in the past 2-3 months. However, glycated hemoglobin cannot determine the patient's level of glycemic control in the short term, nor can the effect of the medication be monitored in the short term.
For the above reasons, in clinical examination, the above three indexes are generally required to be jointly detected so as to assist judgment. However, at present, tests of blood sugar, fructosamine and glycosylated hemoglobin need to be performed on different test platforms, for example, blood sugar needs to be performed on a biochemical analyzer, and glycosylated hemoglobin needs to be performed on a glycosylated hemoglobin analyzer based on an HPLC technology, so that the detection efficiency is low; moreover, the blood glucose test sample properties are serum and plasma, and the glycated hemoglobin sample properties are whole blood, which requires that the patient be sampled several times.
Accordingly, the prior art is yet to be improved and developed.
Disclosure of Invention
The invention aims to solve the technical problem that in the prior art, a multi-item detection all-in-one machine is provided to solve the problems that tests of blood sugar, fructosamine and glycosylated hemoglobin need to be detected on different platforms and blood sampling of a patient needs to be carried out for multiple times during detection.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a multi-item detection all-in-one machine comprises an electrochemical detection mechanism, a high-pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used for detecting the contents of blood sugar and fructosamine, and the high-pressure liquid chromatography mechanism is used for detecting the content of glycosylated hemoglobin;
the sample adding mechanism is connected with the high-pressure liquid chromatography mechanism and used for conveying a sample solution to the high-pressure liquid chromatography mechanism and dropping the sample solution into the electrochemical detection mechanism.
The integrated multi-item detection machine comprises a sample adding mechanism, a sample adding mechanism and a sample collecting mechanism, wherein the sample adding mechanism comprises an electromagnetic valve group, an injector, a sample adding needle, a hemolysis pool and a solution tank, a hemolytic agent or a cleaning agent is arranged in the solution tank, and the injector, the sample adding needle, the solution tank and the hemolysis pool are all connected with the electromagnetic valve group through pipelines; and the injector is communicated with the sample adding needle, or the injector is communicated with the solution tank, or the injector is communicated with the hemolysis pool by switching the electromagnetic valve group.
The multi-item detection all-in-one machine is characterized in that the electromagnetic valve group comprises a first electromagnetic valve, a second electromagnetic valve and a third electromagnetic valve, a first pipeline is connected between the first electromagnetic valve and the second solution tank, a second pipeline is connected between the second electromagnetic valve and the sample adding needle, and a third pipeline is connected between the third electromagnetic valve and the hemolyzing pool;
the injector is connected with a fourth pipeline, and the first electromagnetic valve, the second electromagnetic valve and the third electromagnetic valve are all connected with one end, far away from the injector, of the fourth pipeline.
The multi-item detection all-in-one machine is characterized in that the electromagnetic valve group further comprises a fourth electromagnetic valve, a cleaning piece is arranged on the sample adding needle, and a fifth pipeline is connected between the fourth electromagnetic valve and the cleaning piece;
and the injector is used for filling cleaning liquid into the cleaning piece through the fifth pipeline so as to clean the blood on the sampling needle.
The multi-item detection all-in-one machine is characterized in that a waste liquid pool is connected to the bottom of the blood dissolving pool.
The all-in-one multi-item detection machine is characterized in that the high-pressure liquid chromatography mechanism comprises a high-pressure pump, a sample ring and a glycosylated hemoglobin detection device which are sequentially connected;
the sample ring is connected with the second pipeline, and the injector sucks the sample solution into the sample ring by switching to be communicated with the second pipeline.
The all-in-one machine for detecting multiple items is characterized in that the glycosylated hemoglobin detecting device comprises a chromatographic column and a detecting module, and the sample ring, the chromatographic column and the detecting module are sequentially connected.
The integrated multi-item detection machine comprises a high-pressure liquid chromatography mechanism, a sample valve, a first passage, a second passage, a third passage, a fourth passage, a fifth passage and a sixth passage, wherein the sample valve is sequentially provided with the first valve, the second valve, the third valve, the fourth valve, the fifth valve and the sixth valve;
the second pipeline comprises a first pipeline section and a second pipeline section, one end of the first pipeline section is connected with the second electromagnetic valve, the other end of the first pipeline section is connected with the first valve, one end of the second pipeline section is connected with the second valve, and the other end of the second pipeline section is connected with the sample adding needle;
a first high-pressure pipeline is connected between the high-pressure pump and the fifth valve, a first loop is connected between the sixth valve and the sample ring, a second loop is connected between the sample ring and the third valve, and a second high-pressure pipeline is connected between the fourth valve and the glycosylated hemoglobin detection device.
The integrated multi-item detection machine is characterized in that the electrochemical detection mechanism comprises a sample flow cell and an electrolytic cell, the sample flow cell is positioned at the bottom of the electrolytic cell and is communicated with the electrolytic cell through an oxygen semi-permeable membrane, and glucose oxidase or ketoamine enzyme is added into the sample flow cell; the distance between the cathode in the electrolytic cell and the oxygen semipermeable membrane is smaller than the distance between the anode in the electrolytic cell and the oxygen semipermeable membrane.
The multi-project detection all-in-one machine is characterized in that the cathode is made of platinum, the anode is made of Ag or AgCl, and a polarization voltage of 0.6-0.8V is applied between the cathode and the anode.
Has the advantages that: according to the invention, the electrochemical detection mechanism and the high-pressure liquid chromatography mechanism are integrated together, so that the multi-item detection integrated machine can simultaneously detect blood sugar, fructosamine and glycosylated hemoglobin, thereby avoiding the need of performing three detection items on three different instrument platforms, improving the detection efficiency, and simultaneously avoiding the need of performing multiple blood sampling operations on patients when detecting different items.
Drawings
FIG. 1 is a schematic structural diagram of the integrated multi-item detection machine (not including the electrochemical detection mechanism) provided by the present invention;
FIG. 2 is a schematic structural diagram of the electrochemical detection mechanism provided in the present invention;
the labels in the figures are: 1. an injector; 2. a sample adding needle; 3. a blood dissolving pool; 4. an electromagnetic valve group; 41. a first solenoid valve; 42. a second solenoid valve; 43. a third electromagnetic valve; 44. a fourth solenoid valve; 5. a solution tank; 6. a first pipeline; 7. a second pipeline; 71. a first tube section; 72. a second tube section; 8. a third pipeline; 9. a fourth pipeline; 10. a fifth pipeline; 11. a waste liquid tank; 12. a high pressure pump; 13. a sample loop; 14. a glycated hemoglobin detection device; 141. a chromatography column; 142. a detection module; 15. a sample valve; 16. a first valve; 17. a second valve; 18. a third valve; 19. a fourth valve; 20. a fifth valve; 21. a sixth valve; 22. a first path; 23. a second path; 24. a third path; 25. a fourth path; 26. a fifth path; 27. a first high-pressure line; 28. a first loop circuit; 29. a second loop; 30. a second high pressure line; 31. a sample flow cell; 32. an electrolytic cell; 33. an oxygen semipermeable membrane; 34. a cathode; 35. an anode; 36. and (5) cleaning the part.
Detailed Description
The invention provides a multi-item detection all-in-one machine, and in order to make the purpose, technical scheme and effect of the invention clearer and clearer, the invention is further described in detail below by referring to the attached drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It should also be noted that the same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is only for convenience of describing the present invention and simplifying the description, but it is not intended to indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limiting the present patent, and the specific meaning of the terms may be understood by those skilled in the art according to specific circumstances.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
The invention will be further explained by the description of the embodiments with reference to the drawings.
The embodiment provides a multi-project detection all-in-one machine, which comprises an electrochemical detection mechanism, a high-pressure liquid chromatography mechanism and a sample adding mechanism, wherein the sample adding mechanism is connected with the high-pressure liquid chromatography mechanism, the electrochemical detection mechanism is used for detecting the contents of blood sugar and fructosamine, and the high-pressure liquid chromatography mechanism is used for detecting the content of glycosylated hemoglobin; the sample adding mechanism can obtain a blood sample and can obtain a sample solution after dissolving a hemolytic agent and the blood sample; after the sample solution is obtained, the sample adding mechanism is further used for conveying the sample solution to the high-pressure liquid chromatography mechanism so as to detect the content of the glycosylated hemoglobin in the sample solution through the high-pressure liquid chromatography mechanism, and the sample adding mechanism can also drop the sample solution into the electrochemical detection mechanism positioned on one side so as to detect the content of blood sugar and fructosamine in the sample solution through the electrochemical detection mechanism.
According to the invention, the electrochemical detection mechanism and the high-pressure liquid chromatography mechanism are integrated together, so that the multi-item detection integrated machine can simultaneously detect blood sugar, fructosamine and glycosylated hemoglobin, thereby avoiding the need of performing three detection items on three different instrument platforms, improving the detection efficiency, and simultaneously avoiding the need of performing multiple blood drawing operations on patients when detecting different items.
Specifically, as shown in fig. 1, the sample adding mechanism includes an electromagnetic valve group 4, an injector 1, a sample adding needle 2, a hemolysis cell 3, and a solution tank 5, wherein the solution tank 5 is used for containing a hemolytic agent or a cleaning agent, and the injector 1, the sample adding needle 2, the solution tank 5, and the hemolysis cell 3 are all connected to the electromagnetic valve group 4 through a pipeline; by switching the solenoid valve group 4, the syringe 1 can be communicated with the sampling needle 2, or the syringe 1 can be communicated with the solution tank 5, or the syringe 1 can be communicated with the hemolyzing cell 3, that is, the syringe can be communicated with one of the sampling needle 2, the hemolyzing cell 3, and the solution tank 5 by switching the solenoid valve group. When the sample solution needs to be generated, the solenoid valve set 4 is firstly switched to the injector 1 to be communicated with the sample needle, the injector 1 sucks the blood sample into the sample adding needle 2, then the injector 1 adds the blood sample in the sample adding needle 2 into the hemolyzing pool 3, and then the electromagnetic valve group 4 is switched, so that the syringe 1 communicates with the solution tank 5, the syringe 1 sucks up the hemolytic agent in the solution tank 5, then the electromagnetic valve set 4 is switched to the injector 1 to be communicated with the hemolyzing pool 3, the injector 1 discharges the sucked hemolytic agent into the hemolyzing pool 3 to be mixed with the blood sample in the hemolyzing pool 3, thereby dissolving out hemoglobin in the red blood cells in the blood sample and finally forming the sample solution. Further, when sample adding detection is required, the electromagnetic valve group 4 is switched to the injector 1 to be communicated with the sample adding needle 2, and the sample solution is sucked into the high pressure liquid chromatography mechanism through the injector 1, or the sample solution is sucked and dropped into the electrochemical detection mechanism.
Further, the electromagnetic valve group 4 comprises a first electromagnetic valve 41, a second electromagnetic valve 42 and a third electromagnetic valve 43, and a first pipeline 6 is connected between the first electromagnetic valve 41 and the second solution tank 5; a second pipeline 7 is connected between the second electromagnetic valve 42 and the sample adding needle 2, and a third pipeline 8 is connected between the third electromagnetic valve 43 and the hemolyzing pool 3; the injector 1 is connected with a fourth pipeline 9, and the first electromagnetic valve 41, the second electromagnetic valve 42 and the third electromagnetic valve 43 are all connected with one end of the fourth pipeline 9 far away from the injector 1. When the first solenoid valve 41 is switched to the fourth pipeline 9 to communicate with the first pipeline 6, the syringe 1 can suck up the hemolytic agent from the solution tank 5; when the second solenoid valve 42 is switched to the fourth pipeline 9 to communicate with the second pipeline 7, the injector 1 can suck the blood sample into the sample adding needle 2, or push the blood sample from the sample adding needle 2 into the hemolyzing chamber 3; when the third solenoid valve 43 is switched to connect the fourth line 9 and the third line 8, the syringe 1 can add the hemolytic agent sucked into the syringe 1 into the hemolyzing pool 3, and can continuously suck the liquid in the hemolyzing pool 3 into the third line 8 and timely discharge the liquid from the third line 8 into the hemolyzing pool 3 after the hemolytic agent is added into the blood sample in the hemolyzing pool 3, so that the hemoglobin in the hemolyzing pool 3 can be more completely dissolved by continuous sucking and discharging actions.
The electromagnetic valve group 4 further comprises a fourth electromagnetic valve 44, a cleaning piece 36 is arranged on the sample adding needle 2, and a fifth pipeline 10 is connected between the fourth electromagnetic valve 44 and the cleaning piece 36; since some blood is inevitably left on the outer wall of the end of the sample application needle 2 after the sample application needle 2 sucks the blood sample, in order to refresh the sample application needle 2, the syringe 1 fills the cleaning material 36 with the cleaning solution through the fifth pipeline 10 to clean the blood on the sample application needle 2, that is, the first electromagnetic valve 41 is switched to the first pipeline 6 to communicate with the fourth pipeline 9, the syringe 1 sucks the cleaning solution from the solution tank 5, then the fourth electromagnetic valve 44 is switched to the fifth pipeline 10 to communicate with the fourth pipeline 9, and the syringe 1 pushes the cleaning solution into the cleaning material 36 to clean the blood on the sample application needle 2.
The bottom of the hemolysis pool 3 is connected with a waste liquid pool 11, so that after the detection of various biological indexes is finished, the residual liquid in the hemolysis pool 3 is discharged into the waste liquid pool 11.
The high-pressure liquid chromatography mechanism comprises a high-pressure pump 12, a sample ring 13 and a glycosylated hemoglobin detection device 14 which are sequentially connected; the sample ring 13 is connected with the second pipeline 7, and the syringe 1 sucks the sample solution into the sample ring 13 by switching to be communicated with the second pipeline 7; specifically, when the content of glycated hemoglobin needs to be measured, the second solenoid valve 42 is switched to the fourth line 9 to communicate with the second line 7, the syringe 1 sucks the sample solution from the dissolution tank into the sample loop 13, and then the high-pressure pump 12 pushes the sample solution in the sample loop 13 into the glycated hemoglobin measuring apparatus 14 for measurement.
The glycated hemoglobin measurement device 14 includes a chromatography column 141 and a measurement module 142, and the sample loop 13, the chromatography column 141, and the measurement module 142 are connected in this order; after the sample solution is pushed into the chromatographic column 141 by the eluent pumped by the high-pressure pump 12, the sample solution is chromatographed by the chromatographic column 141, and the chromatographed liquid enters the detection module 142 for detection, so as to obtain the content of the glycated hemoglobin. Further, the detection module 142 may be connected to the waste liquid tank 11 to discharge the liquid after the detection of the glycated hemoglobin into the waste liquid tank 11.
The high-pressure liquid chromatography mechanism further comprises a sample valve 15, wherein a first valve 16, a second valve 17, a third valve 18, a fourth valve 19, a fifth valve 20 and a sixth valve 21 are sequentially arranged on the sample valve 15, a first passage 22 is connected between the first valve 16 and the second valve 17, a second passage 23 is connected between the second valve 17 and the third valve 18, a third passage 24 is connected between the third valve 18 and the fourth valve 19, a fourth passage 25 is connected between the fifth valve 20 and the sixth valve 21, and a fifth passage 26 is connected between the sixth valve 21 and the first valve 16; the second pipeline 7 comprises a first pipeline section 71 and a second pipeline section 72, one end of the first pipeline section 71 is connected with the second electromagnetic valve 42, the other end of the first pipeline section is connected with the first valve 16, one end of the second pipeline section 72 is connected with the second valve 17, and the other end of the second pipeline section 72 is connected with the sampling needle 2; a first high-pressure line 27 is connected between the high-pressure pump 12 and the fifth valve 20, a first loop 28 is connected between the sixth valve 21 and the sample ring 13, a second loop 29 is connected between the sample ring 13 and the third valve 18, and a second high-pressure line 30 is connected between the fourth valve 19 and the glycated hemoglobin measurement device 14.
By switching the first valve 16, the second valve 17, the third valve 18, the fourth valve 19, the fifth valve 20, and the sixth valve 21, the communication of the sample loop 13 with the second line 7, the communication of the sample loop 13 with the high-pressure pump 12, or the communication of the first passage 22 with the second line 7 can be achieved. Specifically, when a blood sample needs to be obtained by using the sample addition needle 2 or filled into the cuvette 3, the second battery valve is switched to communicate with the fourth pipeline 9 and the second pipeline 7, and the first valve 16 and the second valve 17 need to be switched, so that the first pipe section 71, the first passage 22, and the second pipe section 72 are sequentially communicated, so as to suck the blood into the sample addition needle 2 through the syringe 1 or discharge the blood in the sample addition needle 2 into the cuvette 3; when the sample solution in the cuvette 3 is to be sucked into the sample loop 13 by using the sampling needle 2, the first valve 16, the second valve 17, the third valve 18 and the sixth valve 21 are switched to be communicated with the first pipe section 71, the fifth passage 26, the first loop 28, the second loop 29, the second passage 23 and the second pipe section 72 while the second battery valve to the fourth pipe 9 is switched to be communicated with the second pipe 7, so that the sample solution is sucked into the sample loop 13 from the solution cuvette by the syringe 1 and the sampling needle 2; further, when the sample solution in the sample loop 13 needs to be transferred to the glycated hemoglobin measurement apparatus 14, the third valve 18, the fourth valve 19, the fifth valve 20, and the sixth valve 21 are switched to be connected to the first high-pressure line 27, the fourth line 25, the first loop 28, the second loop 29, the third line 24, and the second high-pressure line 30 in sequence, so that the high-pressure pump 12 pumps the eluent into the sample loop 13 and pushes the sample solution in the sample loop 13 into the glycated hemoglobin measurement apparatus 14.
Since the pressure in the first high-pressure pipeline 27 and the second high-pressure pipeline 30 is usually high, and the second pipeline 7 is a vacuum pipeline, the sample valve 15 is arranged to switch pipelines, and the effect of isolating the pressure is achieved.
As shown in fig. 2, the electrochemical detection mechanism comprises a sample flow cell 31 and an electrolytic cell 32, wherein the sample flow cell 31 is positioned at the bottom of the electrolytic cell 32, the sample flow cell 31 is communicated with the electrolytic cell 32 through an oxygen semi-permeable membrane 33, and glucose oxidase or ketoamine enzyme is added into the sample flow cell 31; the distance between the cathode 34 and the oxygen semipermeable membrane 33 in the electrolytic cell 32 is smaller than the distance between the anode 35 and the oxygen semipermeable membrane 33 in the electrolytic cell 32, that is, the cathode 34 is close to the oxygen semipermeable membrane 33, and the anode 35 is far away from the oxygen semipermeable membrane 33. In one embodiment, the cathode 34 is platinum, the anode 35 is Ag or AgCl, and a polarization voltage of 0.6V to 0.8V is applied between the cathode 34 and the anode 35. In the platinum-Ag/AgCl electrolytic cell 32, the platinum cathode 34 has a small area, which is several tens to several hundredths of the Ag/AgCl anode 35, a stable polarization voltage of 0.6-0.8V is applied between the platinum-Ag/AgCl electrodes, and oxygen in the sample flow cell 31 passes through the oxygen semi-permeable membrane 33 to the surface of the cathode 34 to be reduced, so that an oxidation-reduction reaction occurs, wherein the reduction reaction formula is as follows: o2+4e +2H2O- - -4OH ", generates an electrolytic current such that the surface of the cathode 34 has a much lower oxygen concentration than the surface of the anode 35, and concentration diffusion occurs, and when the diffusion concentration is relatively stable, a stable electrolytic current, also called a limiting diffusion current, is generated, the magnitude of which depends on the amount of oxygen permeating to the surface of the cathode 34, and the amount of oxygen permeating to the surface of the cathode 34 depends on the amount of oxygen in the sample flow cell 31.
For example, when the content of glucose or fructosamine in a sample solution needs to be detected, the second battery valve is switched to connect the fourth pipeline 9 and the second pipeline 7, and the first valve 16 and the second valve 17 are switched to connect the first pipe section 71, the first passage 22 and the second pipe section 72 in sequence, then the sample solution is sucked into the sample adding needle 2 through the syringe 1, then the sample adding needle 2 is moved to above the flow cell, and the sample solution is dripped into the sample flow cell 31, and when the glucose oxidase is added into the sample flow cell 31, the glucose consumes oxygen under the catalysis of the glucose oxidase, wherein the reaction formula of glucose under the catalysis of GOD (glucose oxidase) is as follows:
when blood glucose consumes oxygen catalyzed by glucose oxidase, oxygen in the sample flow cell 31 will be reduced, and oxygen concentration diffusion will occur at this time, resulting in oxygen in the electrolytic cell 32 diffusing into the sample flow cell 31 through the oxygen semipermeable membrane 33, thereby further resulting in a reduction in oxygen content in the electrolytic cell 32, especially a reduction in oxygen required for the reduction reaction to occur at the cathode 34, and thus the electrolytic current also becomes weak, i.e., the electrolytic current changes; the change of the oxygen content in the electrolytic solution can be measured by measuring the current between the cathode 34 and the anode 35 before and after the change, and the blood sugar content can be further calculated according to the catalysis principle of the blood sugar by the glucose oxidase catalyst after the change of the oxygen content is obtained.
The fructosamine content is detected by adding the ketoamine enzyme as a catalyst into the sample flow cell 31 to consume the oxygen in the sample flow cell 31, so that the change of the oxygen content can be obtained according to the change of the electrolysis current, and further the fructosamine content can be obtained.
The multi-item detection all-in-one machine further comprises a control system, and the control system is used for controlling the switching of each valve in the sample ring 13, the switching of the electromagnetic valve group 4, the movement of the sample adding needle 2 and the operation of the injector 1, the electrochemical reaction mechanism and the high-pressure liquid chromatography mechanism.
In summary, the invention discloses a multi-item detection all-in-one machine, which comprises an electrochemical detection mechanism, a high-pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used for detecting the contents of blood sugar and fructosamine, and the high-pressure liquid chromatography mechanism is used for detecting the content of glycosylated hemoglobin; the sample adding mechanism is connected with the high-pressure liquid chromatography mechanism and used for conveying a sample solution to the high-pressure liquid chromatography mechanism and dropping the sample solution into the electrochemical detection mechanism. According to the invention, the electrochemical detection mechanism and the high-pressure liquid chromatography mechanism are integrated together, so that the multi-item detection integrated machine can simultaneously detect blood sugar, fructosamine and glycosylated hemoglobin, thereby avoiding the need of performing three detection items on three different instrument platforms, improving the detection efficiency, and simultaneously avoiding the need of performing multiple blood drawing operations on patients when detecting different items.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. The all-in-one machine for multi-item detection is characterized by comprising an electrochemical detection mechanism, a high-pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used for detecting the contents of blood sugar and fructosamine, and the high-pressure liquid chromatography mechanism is used for detecting the content of glycosylated hemoglobin;
the sample adding mechanism is connected with the high-pressure liquid chromatography mechanism and used for conveying a sample solution to the high-pressure liquid chromatography mechanism and dropping the sample solution into the electrochemical detection mechanism.
2. The integrated multi-item detection machine according to claim 1, wherein the sample adding mechanism comprises an electromagnetic valve set, an injector, a sample adding needle, a hemolysis cell and a solution tank, wherein a hemolytic agent or a cleaning agent is arranged in the solution tank, and the injector, the sample adding needle, the solution tank and the hemolysis cell are all connected with the electromagnetic valve set through pipelines; and the injector is communicated with the sample adding needle, or the injector is communicated with the solution tank, or the injector is communicated with the hemolysis pool by switching the electromagnetic valve group.
3. The multi-item detection integrated machine according to claim 2, wherein the electromagnetic valve group comprises a first electromagnetic valve, a second electromagnetic valve and a third electromagnetic valve, a first pipeline is connected between the first electromagnetic valve and the second solution tank, a second pipeline is connected between the second electromagnetic valve and the sample injection needle, and a third pipeline is connected between the third electromagnetic valve and the hemolyzing cell;
the injector is connected with a fourth pipeline, and the first electromagnetic valve, the second electromagnetic valve and the third electromagnetic valve are all connected with one end, far away from the injector, of the fourth pipeline.
4. The multi-item detection all-in-one machine according to claim 3, wherein the electromagnetic valve group further comprises a fourth electromagnetic valve, a cleaning piece is arranged on the sample adding needle, and a fifth pipeline is connected between the fourth electromagnetic valve and the cleaning piece;
and the injector is used for filling cleaning liquid into the cleaning piece through the fifth pipeline so as to clean the blood on the sampling needle.
5. The multi-item detection all-in-one machine according to claim 3, wherein a waste liquid pool is connected to the bottom of the blood dissolving pool.
6. The integrated multi-item detection machine according to claim 3, wherein the high pressure liquid chromatography mechanism comprises a high pressure pump, a sample ring and a glycosylated hemoglobin detection device which are connected in sequence;
the sample ring is connected with the second pipeline, and the injector sucks the sample solution into the sample ring by switching to be communicated with the second pipeline.
7. The integrated multi-item detection machine according to claim 6, wherein the glycosylated hemoglobin detection device comprises a chromatographic column and a detection module, and the sample ring, the chromatographic column and the detection module are connected in sequence.
8. The integrated multi-item detection machine according to claim 6, wherein the high pressure liquid chromatography mechanism further comprises a sample valve, a first valve, a second valve, a third valve, a fourth valve, a fifth valve and a sixth valve are sequentially arranged on the sample valve, a first passage is connected between the first valve and the second valve, a second passage is connected between the second valve and the third valve, a third passage is connected between the third valve and the fourth valve, a fourth passage is connected between the fifth valve and the sixth valve, and a fifth passage is connected between the sixth valve and the first valve;
the second pipeline comprises a first pipeline section and a second pipeline section, one end of the first pipeline section is connected with the second electromagnetic valve, the other end of the first pipeline section is connected with the first valve, one end of the second pipeline section is connected with the second valve, and the other end of the second pipeline section is connected with the sample adding needle;
a first high-pressure pipeline is connected between the high-pressure pump and the fifth valve, a first loop is connected between the sixth valve and the sample ring, a second loop is connected between the sample ring and the third valve, and a second high-pressure pipeline is connected between the fourth valve and the glycosylated hemoglobin detection device.
9. The integrated multi-item detection machine according to claim 1, wherein the electrochemical detection mechanism comprises a sample flow cell and an electrolytic cell, the sample flow cell is positioned at the bottom of the electrolytic cell and is communicated with the electrolytic cell through an oxygen semi-permeable membrane, and glucose oxidase or ketoamine enzyme is added into the sample flow cell; the distance between the cathode in the electrolytic cell and the oxygen semipermeable membrane is smaller than the distance between the anode in the electrolytic cell and the oxygen semipermeable membrane.
10. The all-in-one multi-item detection machine according to claim 9, wherein the cathode is platinum, the anode is Ag or AgCl, and a polarization voltage of 0.6V-0.8V is applied between the cathode and the anode.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114200070A (en) * | 2021-12-14 | 2022-03-18 | 嘉兴市唯真生物科技有限公司 | Liquid chromatography device |
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