CN114162440A - Golgi protein GP73 quantitative determination kit and detection method thereof - Google Patents

Golgi protein GP73 quantitative determination kit and detection method thereof Download PDF

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Publication number
CN114162440A
CN114162440A CN202111481148.8A CN202111481148A CN114162440A CN 114162440 A CN114162440 A CN 114162440A CN 202111481148 A CN202111481148 A CN 202111481148A CN 114162440 A CN114162440 A CN 114162440A
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China
Prior art keywords
push rod
rod
jacking
pressing
block
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Granted
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CN202111481148.8A
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Chinese (zh)
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CN114162440B (en
Inventor
王熙婷
蒋玉香
张玲
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Zhenjiang Jintai Medical Science Examination Laboratory Co ltd
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Zhenjiang Jintai Medical Science Examination Laboratory Co ltd
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Publication of CN114162440A publication Critical patent/CN114162440A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • B65D25/10Devices to locate articles in containers
    • B65D25/101Springs, elastic lips, or other resilient elements to locate the articles by pressure
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • B65D25/10Devices to locate articles in containers
    • B65D25/107Grooves, ribs, or the like, situated on opposed walls and between which the articles are located
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/38Devices for discharging contents
    • B65D25/52Devices for discharging successive articles or portions of contents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/02Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
    • B65D81/05Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
    • B65D81/07Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents using resilient suspension means
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W90/00Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
    • Y02W90/10Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a quantitative determination kit for Golgi protein GP73 and a detection method thereof, and the quantitative determination kit comprises a box body and a storage bin connected in the box body, wherein storage barrels for storing reagent bottles are uniformly arranged in the storage bin, the storage barrels are connected with a jacking mechanism for jacking the reagent bottles, the storage bin is connected with a driving mechanism for driving a push rod to ascend, the driving mechanism comprises a push rod I and a jacking block connected to one end of the push rod I, the storage barrels are connected with a pressing mechanism for pressing the bottle body, the jacking block is separated from a jacking hole arranged on the push rod, the storage barrel is provided with a through hole, and an anti-skid pressing block penetrates through the through hole and is pressed on the bottle body; when the reagent bottle is in a jacking state, the jacking block is connected with a jacking hole arranged on the jacking rod, and the anti-skid pressing block is separated from the bottle body. The reagent bottle storage device can improve the stability of reagent bottles, is not easy to loosen, is convenient for transferring a reagent box, is convenient for taking out the reagent bottles from the storage barrel, and is flexible and convenient to operate.

Description

Golgi protein GP73 quantitative determination kit and detection method thereof
Technical Field
The invention relates to the technical field of kits, in particular to a quantitative determination kit for Golgi protein GP73 and a detection method thereof.
Background
One protein family in the Golgi apparatus, including Golgi protein-67, Golgi protein-95, Golgi protein-245, Golgi protein-160 and peripheral membrane protein gm130, all have coiled-coil structures that form the exoskeletons of the Golgi apparatus. In apoptosis, it is degraded by caspases. And the quantitative determination of the Golgi protein GP73 needs to use a kit, so that the reagent is convenient to store.
When the existing kit is used, the stored reagent bottles are poor in stability, can shake in the way of transportation, and are inconvenient to take and poor in practicability.
Disclosure of Invention
The invention aims to provide a quantitative determination kit for Golgi protein GP73 and a detection method thereof, which have the advantages of improving the stability of a reagent bottle, facilitating transportation and taking, and solve the problems of poor stability of the reagent bottle, shaking in the transportation process and inconvenience in taking.
In order to achieve the purpose, the invention provides the following technical scheme: a Golgi protein GP73 quantitative determination kit comprises a kit body and a storage bin connected in the kit body, wherein storage barrels for storing reagent bottles are uniformly installed in the storage bin, the storage barrels are connected with a jacking mechanism for jacking the reagent bottles, the jacking mechanism comprises a mandril, the storage bin is connected with a driving mechanism for driving the mandril to ascend, the driving mechanism comprises a first push rod and a jacking block connected to one end of the first push rod, the storage barrels are connected with a pressing mechanism for pressing the bottle body, the pressing mechanism comprises a second push rod, an anti-skidding pressing block and a pressing rod, one end of the second push rod is connected to the first push rod, and the other end of the second push rod is connected to the pressing rod;
when the reagent bottle is in a locked state, the jacking block is separated from a jacking hole arranged on the jacking rod, the storage barrel is provided with a through hole, and the anti-skid pressing block penetrates through the through hole and is pressed on the bottle body;
when the reagent bottle is in a jacking state, the jacking block is connected with a jacking hole arranged on the jacking rod, and the anti-skid pressing block is separated from the bottle body.
Preferably, actuating mechanism still includes movable block, spring two, fixing base, movable block sliding connection is in the top of storage storehouse, spring two is used for driving the movable block to removing to the direction that is close to the fixing base, the both ends of spring two are fixed continuous with movable block, fixing base are all fixed respectively, the other end of push rod one is connected in the movable block.
Preferably, the jacking mechanism further comprises a third spring and a spring seat, the third spring is used for driving the ejector rod to move towards the spring seat, the spring seat is fixed on the storage barrel, and two ends of the third spring are respectively and fixedly connected with the ejector rod and the spring seat.
Preferably, the storage barrel is connected with a guide plate for guiding the ejector rod, and the guide plate is connected with the ejector rod in a sliding manner.
Preferably, the pressing mechanism further comprises a supporting rod and a first spring, one end of the supporting rod is fixed to the storage barrel, the other end of the supporting rod is hinged to the pressing rod, the first spring is used for driving the anti-skidding pressing block to move towards the direction of the through hole, and the anti-skidding pressing block is connected to one end of the pressing rod.
Preferably, the other end of the second push rod is hinged to a sliding block which is slidably connected to the pressing rod, and two ends of the first spring are fixedly connected with the storage barrel and the other end of the pressing rod respectively.
Preferably, the movable block is connected with a protrusion for pushing the movable block to move in a reciprocating manner.
Preferably, the top of the storage cylinder is provided with an opening matched with the bottle cap.
Preferably, the box cover further comprises a handle connected to the top of the box cover, and the box cover is hinged to the box body.
A detection method of a Golgi protein GP quantitative determination kit comprises the following steps:
the method comprises the following steps: and opening the box cover, inserting the reagent bottle into the storage barrel, and positioning the bottle cover in the opening.
Step two: the second spring pulls the movable block to be close to the direction of fixing base, and then pulls push rod one, the push rod two to the direction of keeping away from the storage cylinder remove, and the kicking block breaks away from with the ejector pin mutually, and the other end of pressing the depression bar is pushed to the first spring, presses the non-slip briquetting of depression bar drive to compress tightly in the body.
Step three: promote two movable blocks and be close to each other, make push rod one, the push rod two to the direction that is close to the storage cylinder remove, the kicking block is connected in the jack-up hole, along with two movable block intervals reduce, the kicking block is with the high improvement of ejector pin jack-up, the bottle lid by the jack-up, meanwhile, press the anti-skidding briquetting of pole drive to keeping away from the body direction and remove, can hand the bottle lid and take out the reagent bottle from the storage cylinder.
Compared with the prior art, the invention has the following beneficial effects: according to the reagent bottle storage device, the jacking mechanism, the driving mechanism and the pressing mechanism are arranged, the jacking mechanism comprises the ejector rod, the spring III and the spring seat, the driving mechanism comprises the push rod I, the ejector block, the movable block, the spring II and the fixed seat, the pressing mechanism comprises the push rod II, the anti-skidding pressing block, the pressing rod, the supporting rod and the spring I, when a reagent bottle is positioned in the storage barrel, the spring II is used for driving the movable block to move towards the direction close to the fixed seat, the push rod I and the push rod are further pulled to move towards the direction far away from the storage barrel, the anti-skidding pressing block at one end of the pressing rod is pressed on the bottle body, and meanwhile, the use of the spring I is matched, so that the stability of the reagent bottle is improved, the reagent bottle is not easy to loosen, the reagent bottle storage device is safe and reliable to use, and the reagent box is convenient to transport; when the reagent bottle needs to be taken out, the two movable blocks are pushed to be close to each other, the ejector blocks are connected to the jacking holes of the ejector rods and jack up the ejector rods, the bottle caps at the opening parts are jacked up by the ejector rods, meanwhile, the anti-skidding pressing blocks are separated from the bottle body, the reagent bottle is convenient to take out of the storage barrel, operation is flexible and convenient, and practicability is high.
The storage barrel is connected with a guide plate used for guiding the ejector rod, and the guide plate is connected with the ejector rod in a sliding mode, so that the ejector rod is lifted and stabilized, the skew deviation is prevented, and the stability of the reagent bottle when being jacked up is improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of a compact configuration of a reagent bottle according to the present invention;
FIG. 3 is an enlarged view taken at A in FIG. 2;
FIG. 4 is an enlarged view of FIG. 2 at B;
FIG. 5 is an enlarged view at C of FIG. 2;
FIG. 6 is a schematic view of the cartridge of the present invention;
fig. 7 is a schematic structural diagram of a conventional reagent bottle.
In the figure: 1. a box body; 2. a box cover; 3. a storage bin; 4. a storage drum; 5. a first push rod; 6. a top block; 7. a bottle cap; 8. a guide plate; 9. a top rod; 10. a movable block; 11. an anti-skid briquette; 12. a pressing lever; 13. a second push rod; 14. a strut; 15. a first spring; 16. a bottle body; 17. a second spring; 18. a fixed seat; 19. a protrusion; 20. a through hole; 21. a slider; 22. jacking up the hole; 23. a third spring; 24. a spring seat; 25. an opening; 26. a handle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 7, a conventional reagent bottle includes a bottle body 16 and a bottle cap 7 connected to a top of the bottle body 16.
Referring to fig. 1 to 6, the present invention provides a quantitative determination kit for golgi protein GP73, which includes a box body 1 and a storage bin 3 connected to the box body 1, wherein the storage bin 3 is made of heat insulation material and has heat insulation effect on a reagent bottle. Storage barrel 4 for storing reagent bottles is uniformly installed in the storage bin 3, the storage barrel 4 is connected with a jacking mechanism for jacking the reagent bottles, the jacking mechanism is arranged relative to the storage barrel 4, and the jacking mechanism comprises a jacking rod 9. The storage bin 3 is connected with a driving mechanism used for driving the ejector rod 9 to ascend, the driving mechanism comprises a first push rod 5 and an ejector block 6 connected to one end of the first push rod 5, the cross section of the ejector block 6 is of a triangular structure, and when the ejector rod 9 is jacked, the inclined surface of the ejector block 6 is connected with the top end surface of the jacking hole 22. The storage barrel 4 is connected with a pressing mechanism used for pressing the bottle body 16, the pressing mechanism comprises a second push rod 13, an anti-skidding pressing block 11 and a pressing rod 12, one end of the second push rod 13 is connected to the first push rod 5, the other end of the second push rod 13 is connected to the pressing rod 12, and the second push rod 13 and the first push rod 5 are both L-shaped structures. When the reagent bottle is in a locked state, the jacking block 6 is separated from the jacking hole 22 arranged on the jacking rod 9, the storage barrel 4 is provided with a through hole 20, and the anti-skid pressing block 11 penetrates through the through hole 20 and is pressed on the bottle body 16. The second spring 17 is used for driving the movable block 10 to move towards the direction close to the fixed seat 18, and then the first pulling push rod 5 and the second pushing rod 13 move towards the direction far away from the storage barrel 4, so that the anti-skidding pressing block 11 pressing one end of the pressing rod 12 is pressed on the bottle body 16, and the first spring 15 is matched for use, so that the stability of the reagent bottle is improved, the reagent bottle is not easy to loosen, the reagent bottle is safe and reliable to use, and the reagent bottle is convenient to transport.
When the reagent bottle is in a jacking state, the jacking block 6 is connected with a jacking hole 22 arranged on the jacking rod 9, and the anti-skid pressing block 11 is separated from the bottle body 16. Promote two movable blocks 10 and be close to each other, make kicking block 6 connect in the jack-up hole 22 of ejector pin 9 to with ejector pin 9 jack-up, ejector pin 9 jacks up the bottle lid 7 of opening 25 department, meanwhile, antiskid briquetting 11 breaks away from mutually with body 16, is convenient for take out the reagent bottle from storage cylinder 4, and the flexible operation is convenient.
The driving mechanism further comprises a movable block 10, a second spring 17 and a fixed seat 18, wherein the movable block 10 is connected to the top of the storage bin 3 in a sliding mode, and the movable block 10 can move stably in a reciprocating mode. The second spring 17 is used for driving the movable block 10 to move towards the direction close to the fixed seat 18, two ends of the second spring 17 are fixedly connected with the movable block 10 and the fixed seat 18 respectively, the other end of the first push rod 5 is connected to the movable block 10, and the first push rod 5 is generally vertically connected with the movable block 10.
The jacking mechanism further comprises a third spring 23 and a spring seat 24, the third spring 23 is used for driving the ejector rod 9 to move towards the spring seat 24, the spring seat 24 is fixed on the storage barrel 4, and two ends of the third spring 23 are fixedly connected with the ejector rod 9 and the spring seat 24 respectively. When the two movable blocks 10 are far away from each other, the third spring 23 enables the ejector rod 9 and the ejector block 6 to be smoothly separated, and the ejector rod 9 is prevented from being blocked.
The storage barrel 4 is connected with a guide plate 8 used for guiding the ejector rod 9, the guide plate 8 is connected with the ejector rod 9 in a sliding mode, the ejector rod 9 is made to ascend and descend stably, skew deviation is prevented, and stability of the reagent bottle when the reagent bottle is jacked up is improved.
The pressing mechanism further comprises a supporting rod 14 and a first spring 15, one end of the supporting rod 14 is fixed to the storage barrel 4, the other end of the supporting rod is hinged to the pressing rod 12, the first spring 15 is used for driving the anti-skidding pressing block 11 to move towards the direction of the through hole 20, and the anti-skidding pressing block 11 is connected to one end of the pressing rod 12.
The other end of the second push rod 13 is hinged to a sliding block 21 which is slidably connected to the pressing rod 12, and two ends of the first spring 15 are fixedly connected with the other ends of the storage barrel 4 and the pressing rod 12 respectively. When the two movable blocks 10 approach each other, the sliding block 21 moves to the direction of approaching the anti-skid pressing block 11; when the two movable blocks 10 move away from each other, the sliding block 21 moves toward the first spring 15.
The movable block 10 is connected with a protrusion 19 for pushing the movable block 10 to reciprocate, and the protrusion 19 is provided to facilitate the operation of the movable block 10.
The top of the storage barrel 4 is provided with an opening 25 matched with the bottle cap 7.
Still include lid 2 and connect in the handle 26 at lid 2 top, lid 2 is articulated with box body 1, can lift handle 26 and be convenient for carry the transportation of kit.
A detection method of a Golgi protein GP73 quantitative determination kit comprises the following steps:
the method comprises the following steps: the lid 2 is opened and the reagent bottle is inserted into the cartridge 4 with the cap 7 in the opening 25.
Step two: the second spring 17 pulls the movable block 10 to approach the fixed seat 18, and then pulls the first push rod 5 and the second push rod 13 to move in the direction away from the storage barrel 4, the ejector block 6 is separated from the ejector rod 9, the first spring 15 pushes the other end of the pressing rod 12, and the pressing rod 12 drives the anti-skidding pressing block 11 to be pressed on the bottle body 16.
Step three: promote two movable blocks 10 and be close to each other, make push rod one 5, push rod two 13 to the direction removal of being close to storage cylinder 4, kicking block 6 is connected in jack-up hole 22, and along with two movable block 10 intervals reduce, the high improvement of kicking block 6 with ejector pin 9 jack-up, bottle lid 7 is by the jack-up, meanwhile, press 12 drive antiskid briquetting 11 to keeping away from the 16 direction removal of body, can hand bottle lid 7 and take out the reagent bottle from storage cylinder 4.
The working principle is as follows: when reagent bottles need to be stored, the box cover 2 is opened, and the reagent bottles are inserted into the corresponding storage cylinders 4, so that the bottle covers 7 are positioned in the openings 25. Two 17 springs are used for driving the movable block 10 to the direction removal that is close to fixing base 18, and movable block 10 sliding connection is in the top of storage storehouse 3, the both ends of push rod 5 respectively with kicking block 6, the movable block 10 links to each other, the one end of push rod two 13 is connected in push rod 5 simultaneously, the other end is articulated in the sliding block 21 of pressing down the depression bar 12 with sliding connection, spring two 17 pulling push rod 5, push rod two 13 move to the direction of keeping away from storage barrel 4, make the anti-skidding briquetting 11 of pressing down bar 12 one end compress tightly in body 16, and spring one 15 is used for driving anti-skidding briquetting 11 and removes to the direction of through-hole 20, and then improve the stability of reagent bottle, difficult not hard up, safe and reliable in utilization, be convenient for the transportation to the kit. When needing to take out the reagent bottle, promote two movable blocks 10 and be close to each other, push rod one 5, two 13 of push rod are to the direction removal of being close to storage cylinder 4, kicking block 6 is connected in jack-up hole 22, along with two movable blocks 10 intervals reduce, the kicking block 6 is with the high improvement of ejector pin 9 jack-up, bottle lid 7 is by the jack-up to press 12 separation of drive antiskid briquetting and body 16 of the pressure bar, be convenient for take out the reagent bottle from storage cylinder 4, the flexible operation is convenient, therefore, the clothes hanger is strong in practicability.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. A quantitative determination kit for Golgi protein GP73, which is characterized in that: the reagent bottle storage box comprises a box body (1) and a storage bin (3) connected in the box body (1), wherein storage barrels (4) used for storing reagent bottles are uniformly installed in the storage bin (3), the storage barrels (4) are connected with a jacking mechanism used for jacking the reagent bottles, the jacking mechanism comprises a push rod (9), the storage bin (3) is connected with a driving mechanism used for driving the push rod (9) to ascend, the driving mechanism comprises a push rod I (5) and a jacking block (6) connected to one end of the push rod I (5), the storage barrels (4) are connected with a pressing mechanism used for pressing the bottle body (16), the pressing mechanism comprises a push rod II (13), an anti-skidding pressing block (11) and a pressing rod (12), one end of the push rod II (13) is connected to the push rod I (5), and the other end of the push rod II (13) is connected to the pressing rod (12);
when the reagent bottle is in a locked state, the jacking block (6) is separated from a jacking hole (22) arranged on the jacking rod (9), the storage barrel (4) is provided with a through hole (20), and the anti-skid pressing block (11) penetrates through the through hole (20) and is pressed on the bottle body (16);
when the reagent bottle is in a jacking state, the jacking block (6) is connected with a jacking hole (22) arranged on the jacking rod (9), and the anti-skid pressing block (11) is separated from the bottle body (16).
2. The quantitative determination kit for Golgi protein GP73 according to claim 1, wherein the driving mechanism further comprises a movable block (10), a second spring (17) and a fixed seat (18), the movable block (10) is slidably connected to the top of the storage bin (3), the second spring (17) is used for driving the movable block (10) to move towards the direction close to the fixed seat (18), two ends of the second spring (17) are respectively fixedly connected to the movable block (10) and the fixed seat (18), and the other end of the first push rod (5) is connected to the movable block (10).
3. The quantitative determination kit for Golgi protein GP73 according to claim 1, wherein the jacking mechanism further comprises a third spring (23) and a spring seat (24), the third spring (23) is used for driving the ejector rod (9) to move towards the spring seat (24), the spring seat (24) is fixed on the storage barrel (4), and two ends of the third spring (23) are respectively and fixedly connected with the ejector rod (9) and the spring seat (24).
4. The kit for the quantitative determination of the Golgi protein GP73 according to claim 3, wherein the storage barrel (4) is connected with a guide plate (8) for guiding the push rod (9), and the guide plate (8) is slidably connected with the push rod (9).
5. The quantitative determination kit for Golgi protein GP73 and the detection method thereof according to claim 1, wherein the pressing mechanism further comprises a supporting rod (14) and a first spring (15), one end of the supporting rod (14) is fixed on the storage barrel (4), the other end of the supporting rod is hinged to the pressing rod (12), the first spring (15) is used for driving the anti-skid pressing block (11) to move towards the direction of the through hole (20), and the anti-skid pressing block (11) is connected to one end of the pressing rod (12).
6. The quantitative determination kit for Golgi protein GP73 according to claim 5, wherein the other end of the second push rod (13) is hinged to a slide block (21) which is slidably connected to the pressing rod (12), and both ends of the first spring (15) are fixedly connected to the other ends of the storage barrel (4) and the pressing rod (12), respectively.
7. The quantitative determination kit for Golgi protein GP73 according to claim 2, wherein the movable block (10) is connected with a protrusion (19) for pushing the movable block (10) to reciprocate.
8. The quantitative determination kit for Golgi protein GP73 according to claim 1, wherein the top of the storage barrel (4) is provided with an opening (25) matched with the bottle cap (7).
9. The quantitative determination kit for Golgi protein GP73 according to claim 1, further comprising a box cover (2) and a handle (26) connected to the top of the box cover (2), wherein the box cover (2) is hinged with the box body (1).
10. A detection method of a Golgi protein GP73 quantitative determination kit is characterized in that: comprises the following steps:
the method comprises the following steps: the box cover (2) is opened, the reagent bottle is inserted into the storage barrel (4), and the bottle cover (7) is positioned in the opening (25);
step two: the second spring (17) pulls the movable block (10) to approach the fixed seat (18) and further pulls the first push rod (5) and the second push rod (13) to move away from the storage barrel (4), the ejector block (6) is separated from the ejector rod (9), the first spring (15) pushes the other end of the pressing rod (12), and the pressing rod (12) drives the anti-skidding pressing block (11) to be pressed on the bottle body (16);
step three: promote two movable blocks (10) and be close to each other, make push rod (5), push rod two (13) remove to the direction of being close to storage cylinder (4), kicking block (6) are connected in jack-up hole (22), along with two movable block (10) intervals reduce, the high improvement of ejector pin (9) jack-up is with kicking block (6), bottle lid (7) are by the jack-up, meanwhile, press the removal of depression bar (12) drive antiskid briquetting (11) to keeping away from body (16) direction, can hand bottle lid (7) and take out the reagent bottle from storage cylinder (4).
CN202111481148.8A 2021-12-06 2021-12-06 Golgi protein GP73 quantitative determination kit and detection method thereof Active CN114162440B (en)

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CN211443243U (en) * 2019-11-18 2020-09-08 济宁医学院附属医院 Place stable hasp formula kit
CN214297006U (en) * 2020-12-26 2021-09-28 中科再生医学科技(广东)有限公司 Stem cell is with storing cabinet convenient to remove transportation
CN214730960U (en) * 2021-04-07 2021-11-16 安徽银坦生物科技有限公司 Medical kit for safe storage

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CN211443243U (en) * 2019-11-18 2020-09-08 济宁医学院附属医院 Place stable hasp formula kit
CN214297006U (en) * 2020-12-26 2021-09-28 中科再生医学科技(广东)有限公司 Stem cell is with storing cabinet convenient to remove transportation
CN214730960U (en) * 2021-04-07 2021-11-16 安徽银坦生物科技有限公司 Medical kit for safe storage

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