CN114158368A - 一种野葛嫩枝扦插的方法 - Google Patents
一种野葛嫩枝扦插的方法 Download PDFInfo
- Publication number
- CN114158368A CN114158368A CN202111362280.7A CN202111362280A CN114158368A CN 114158368 A CN114158368 A CN 114158368A CN 202111362280 A CN202111362280 A CN 202111362280A CN 114158368 A CN114158368 A CN 114158368A
- Authority
- CN
- China
- Prior art keywords
- cuttage
- cutting
- pueraria lobata
- soaking
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005520 cutting process Methods 0.000 title claims abstract description 43
- 235000010575 Pueraria lobata Nutrition 0.000 title claims abstract description 38
- 241000219781 Pueraria montana var. lobata Species 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000002791 soaking Methods 0.000 claims abstract description 30
- 239000011159 matrix material Substances 0.000 claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 10
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 10
- 239000006013 carbendazim Substances 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 10
- 239000012286 potassium permanganate Substances 0.000 claims description 8
- 239000004576 sand Substances 0.000 claims description 8
- 229910052902 vermiculite Inorganic materials 0.000 claims description 8
- 239000010455 vermiculite Substances 0.000 claims description 8
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 6
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 6
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 6
- 229930182555 Penicillin Natural products 0.000 claims description 6
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 6
- 229930003268 Vitamin C Natural products 0.000 claims description 6
- 229910052796 boron Inorganic materials 0.000 claims description 6
- 229940049954 penicillin Drugs 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 235000019154 vitamin C Nutrition 0.000 claims description 6
- 239000011718 vitamin C Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000002262 irrigation Effects 0.000 claims description 4
- 238000003973 irrigation Methods 0.000 claims description 4
- 230000000877 morphologic effect Effects 0.000 claims description 4
- 239000002985 plastic film Substances 0.000 claims description 4
- 229920006255 plastic film Polymers 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 2
- 239000002023 wood Substances 0.000 claims description 2
- 238000011084 recovery Methods 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 206010028980 Neoplasm Diseases 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000219780 Pueraria Species 0.000 description 3
- 230000009194 climbing Effects 0.000 description 3
- 230000000249 desinfective effect Effects 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 241000220485 Fabaceae Species 0.000 description 1
- 241001107889 Vinearia Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Chemical class 0.000 description 1
- SFMJNHNUOVADRW-UHFFFAOYSA-N n-[5-[9-[4-(methanesulfonamido)phenyl]-2-oxobenzo[h][1,6]naphthyridin-1-yl]-2-methylphenyl]prop-2-enamide Chemical compound C1=C(NC(=O)C=C)C(C)=CC=C1N1C(=O)C=CC2=C1C1=CC(C=3C=CC(NS(C)(=O)=O)=CC=3)=CC=C1N=C2 SFMJNHNUOVADRW-UHFFFAOYSA-N 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 244000209700 shan ge teng Species 0.000 description 1
- 239000003516 soil conditioner Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
本发明公开了一种野葛嫩枝扦插的方法，属于矿山生态环境保护与恢复治理苗木类用材繁育技术领域；所述方法包括如下步骤：选材、浸泡、基质处理、扦插及扦插管理。本发明扦插种苗于两周后开始生根，生根速度快，根系数量众多，并着生根瘤，成活率较高，在较短时间内达到增产的目的。
Description
技术领域
本发明属于矿山生态环境保护与恢复治理苗木类用材繁育技术领域；，具体涉及一种野葛嫩枝扦插的方法。
背景技术
野葛属于豆科葛属的木质落叶藤本植物，在矿山生态环境保护与恢复治理中起着重要作用。其生长量大，攀援高度可达30米，匍匐地面甚至可达百米；其蔓叶重叠，郁闭度可达到100%；其根系发达而强大，深固在土壤中，可有效减轻雨水对地表的冲刷，防止水土流失。其耐旱、耐瘠薄性强；这些特性使其成为矿山生态修复优选的先锋植物之一，
野葛属于多年生豆科类藤本植物，葛根是葛属植物野葛的根茎，是《中华人民共和国药典》规定的正品药材，野葛根中的葛根总黄酮具有降血压、血糖、血脂、抗氧化、清除自由基、增强免疫力，抗癌、抗肿瘤等作用。以往葛根的商品供应主要依靠采集野生植株，过度采挖已导致野生资源日趋减少。而野葛作为一种适用于“白茬山”治理的优良攀援植物品种，矿山生态环境保护与恢复治理领域对其种苗的需求越来种苗越大，为避免采挖野生种苗对环境的破坏，批量快速地获得野葛种苗，服务于矿山生态环境保护与恢复治理领域，寻找一种高效便捷的野葛育苗技术方法就显得十分必要。
发明内容
为了解决上述背景技术中的问题，本发明提供了一种野葛嫩枝扦插的方法，扦插后两周即可生根，生根粗壮均一，根系数量众多，提高了后期葛根的产量和品种。
为实现上述目的，本发明提供如下技术方案：
一种野葛嫩枝扦插的方法，包括如下步骤：
S1、选材：选取2年生以上的野葛母株，取其当年生新梢直径5 mm半木质化枝条作为作为扦插条，插条长度控制在25-30 cm，并保留2个茎节以上，1片小叶，基部节下部分保留2-3 cm长，并剪成45-60度角斜面；
S2、浸泡：将剪取的插条在浸泡液A里面浸泡0.5-1h，取出，洗净沥干，然后将插条的形态学下端在浸泡液B中浸泡1 h，取出；
S3、基质处理：在自动喷灌棚内将混合基质整理成20-40cm厚的扦插床，用0.05-0.2%的高锰酸钾均匀喷灌基质，罩上塑料膜，密闭12-48h；
S4、扦插：扦插前，用清水喷淋基质后，使用木棍在基质上插孔，再将处理好的插条的1/3插入已准备好扦插床内。
S5、扦插管理：扦插完成后，保持扦插床湿度75-85％，温度28℃以下，每隔1周用800-1000倍多菌灵和0.05-0.2%高锰酸钾进行交替灭菌，每隔两周除1次杂草。
进一步地，所述浸泡液A为900-1200倍多菌灵溶液，优选地，所述浸泡液A为1000倍多菌灵溶液。
进一步地，所述浸泡液B由以下原料制备而成：萘乙酸 80-120 mg/L、青霉素80-120 mg/L、硼0.2-0.5 mg/L、维生素C15-30 mg/L、余量为水。
优选地，所述浸泡液B以下原料制备而成：萘乙酸100 mg/L、青霉素100 mg/L、硼0.3 mg/L、维生素C20 mg/L、余量为水。
进一步地，所述基质由河沙、蛭石、草炭土组成，各组分体积比为：V河沙:V蛭石：V草炭土=1:1:1。
本发明具有以下有益效果：
1、本发明提供的野葛嫩枝扦插的方法具有操作简单，处理时间短，在市场应用上更具优势。
2、本发明提供的野葛嫩枝扦插的方法在扦插前先将基质浇透水，用木棍在基质上插孔，减少插条在插入基质时对生根部位进行的机械损伤，提高和保证了扦插种苗的生根率和成活率。
3、本发明提供的野葛嫩枝扦插的方法在选材时选择当年生新梢直径5 mm的半木质化枝条，生长健壮，组织充实，腋芽饱满，更能快速收获成熟大苗。
4、浸泡液A对扦插种苗具有消毒杀菌的能力，浸泡液B不仅可以为切口进行消毒杀菌，还能促进插条快速生根，进一步提高和保证了扦插种苗的生根率和成活率。
5、本发明提供的野葛嫩枝扦插的方法，扦插种苗生根速度快，生根粗壮均一，根系数量众多，并着生根瘤，在根瘤内，根瘤菌从豆科植物根的皮层细胞中吸取碳水化合物、矿质盐类及水分，以进行生长和繁殖，同时他们又把游离的氮通过固氮作用固定下来，转变为植物所能利用的含氮化合物，供植物所需，这样，根瘤菌与根就形成了互相依赖的共生关系，提高了后期葛根的产量和质量。
附图说明
图1为实施例1扦插14 d后开始生根局部图片。
图2为实施例1扦插60 d后的图片。
图3为实施例1扦插60 d后的局部根瘤图片。
图4为实施例1扦插60 d后进行移栽，移栽45 d后的图片。
具体实施方式
以下通过具体实施例进一步说明本发明，但本领域技术人员应当知晓本发明的具体实施例并不以任何方式限制本发明，且在本发明基础上所作出的任何等同替换均落入本发明的保护范围。
实施例1：
一种野葛嫩枝扦插的方法，包括以下步骤：
S1、选材：选取2年生以上的野葛母株，取其当年生新梢直径5 mm半木质化枝条作为作为扦插条，插条长度控制在25 cm，并保留2个茎节以上，1片小叶，基部节下部分保留3cm长，剪成45度角斜面；
S2、浸泡：将剪取的插条在浸泡液A里面浸泡0.5 h，取出，洗净沥干，然后将插条的形态学下端在浸泡液B中浸泡1h，取出；其中，浸泡液A由为1000倍多菌灵溶液，浸泡液B由以下原料制备而成：萘乙酸 100 mg/L，青霉素100 mg/L，硼0.3 mg/L，维生素C20 mg/L。
S3、基质处理：在自动喷灌棚内将混合基质整理成30cm厚的扦插床，用0.1%的高锰酸钾均匀喷灌基质，罩上塑料膜，密闭24h后，对基质进行彻底消毒；所述基质由河沙、蛭石、草炭土组成，各组分体积比为：V河沙：V蛭石：V草炭土=1:1:1。
S4、扦插：扦插前，用清水喷淋基质后，使用木棍在基质上插孔，再处理好的插条的1/3插入已准备好苗床内。
S5、扦插管理：扦插完成后，保持扦插床湿度75-85％，温度28℃以下，每隔1周用800-1000倍多菌灵和0.05-0.2%高锰酸钾进行交替灭菌，每隔两周除1次杂草。
实施例2：
一种野葛嫩枝扦插的方法，包括以下步骤：
S1、选材：选取2年生以上的野葛母株，取其当年生新梢直径5 mm半木质化枝条作为作为扦插条，插条长度控制在30 cm，并保留2个茎节以上，1片小叶，基部节下部分保留2cm长，剪成60度角斜面；
S2、浸泡：将剪取的插条在浸泡液A里面浸泡1 h，取出，洗净沥干，然后将插条的形态学下端在浸泡液B中浸泡1.5 h，取出；其中，浸泡液A由为1200倍多菌灵溶液，浸泡液B由以下原料制备而成：萘乙酸120 mg/L、青霉素120 mg/L、硼0.4 mg/L、维生素C30mg/L。
S3、基质处理：在自动喷灌棚内将混合基质整理成30cm厚的扦插床，用0.15%的高锰酸钾均匀喷灌基质，罩上塑料膜，密闭36h；对基质进行彻底消毒；所述基质由河沙、蛭石、草炭土组成，各组分体积比为：V河沙：V蛭石：V草炭土=1:1:1。
S4、扦插：扦插前，用清水喷淋基质后，使用木棍在基质上插孔，再处理好的插条的1/3插入已准备好苗床内。
S5、扦插管理：扦插完成后，保持扦插床湿度75-85％，温度28℃以下，每隔1周用800-1000倍多菌灵和0.05-0.2%高锰酸钾进行交替灭菌，每隔两周除1次杂草。
验证试验：
试验组1 ：
浸泡液A换成纯净水，其余步骤与实施例1完全相同。
试验组2 ：
浸泡液B换成纯净水，其余步骤与实施例1完全相同。
试验组3 ：
浸泡液A和浸泡液B同时换成纯净水，其余步骤与实施例1完全相同：
试验组4：
扦插前，不进行处理，直接将处理好的野葛种苗扦插，其余步骤与实施例1完全相同。
表1野葛种苗培育情况
| 实验组 | 生根速度（d） | 根系数量(株) | 根系长度（mm） | 根系形态 | 生根率 |
| 实施例1 | 14 | 79 | 178.75 | 粗壮、发达 | 81.0% |
| 实施例2 | 20 | 48 | 138.61 | 粗壮、发达 | 76.0% |
| 试验组1 | 17 | 65 | 156.93 | 粗壮、发达 | 65.0% |
| 试验组2 | 45 | 32 | 47.46 | 嫩弱、细小 | 48.0% |
| 试验组3 | 47 | 21 | 42.34 | 嫩弱、细小 | 42.0% |
| 试验组4 | 50 | 18 | 45.21 | 嫩弱、细小 | 45.0% |
Claims (6)
1.一种野葛嫩枝扦插的方法，其特征在于，包括如下步骤：
S1、选材：
选取2年生以上的野葛母株，取其当年生新梢直径5mm半木质化枝条作为扦插条，插条长度控制在25-30 cm，并保留2个以上茎节，1片小叶，基部节下部分保留2-3cm长，并剪成45-60度角斜面；
S2、浸泡：
将剪取的插条在浸泡液A里面浸泡0.5-1h，取出，洗净沥干，然后将插条的形态学下端在浸泡液B中浸泡1h，取出；
S3、基质处理：
在自动喷灌棚内将混合基质整理成20-40cm厚的扦插床，用0.05-0.2%的高锰酸钾均匀喷灌基质，罩上塑料膜，密闭12-48h；
S4、扦插：
扦插前，用清水喷淋基质后，使用木棍在基质上插孔，再将处理好的插条的1/3插入已准备好的扦插床内；
S5、扦插后管理：
扦插完成后，保持扦插床湿度75-85％，温度28℃以下，每隔1周用800-1000倍多菌灵和0.05-0.2%高锰酸钾进行交替灭菌，每隔两周除1次杂草。
2.根据权利要求1所述的野葛嫩枝扦插的方法，其特征在于，所述浸泡液A为900-1200倍多菌灵溶液。
3.根据权利要求2所述的野葛嫩枝扦插的方法，其特征在于，所述浸泡液A为1000倍多菌灵溶液。
4.根据权利要求1所述的野葛嫩枝扦插的方法，其特征在于，所述浸泡液B由以下原料制备而成：萘乙酸80-120mg/L、青霉素80-120mg/L、硼0.2-0.5mg/L、维生素C15-30mg/L，余量为水。
5.根据权利要求4所述的野葛嫩枝扦插的方法，其特征在于，所述浸泡液B由以下原料制备而成：萘乙酸100mg/L、青霉素100mg/L、硼0.3mg/L、维生素C20 mg/L，余量为水。
6.根据权利要求1所述的野葛嫩枝扦插的方法，其特征在于，所述基质由河沙、蛭石、草炭土组成，各组分体积比为：V河沙:V蛭石：V草炭土=1:1:1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111362280.7A CN114158368A (zh) | 2021-11-17 | 2021-11-17 | 一种野葛嫩枝扦插的方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111362280.7A CN114158368A (zh) | 2021-11-17 | 2021-11-17 | 一种野葛嫩枝扦插的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114158368A true CN114158368A (zh) | 2022-03-11 |
Family
ID=80479385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111362280.7A Pending CN114158368A (zh) | 2021-11-17 | 2021-11-17 | 一种野葛嫩枝扦插的方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114158368A (zh) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007089568A (ja) * | 2005-08-30 | 2007-04-12 | Mineaki Nishida | プエラリア・ミリフィカの栽培方法 |
| CN106034722A (zh) * | 2016-07-12 | 2016-10-26 | 青岛农业大学 | 一种金色叶植物扦插繁殖的方法 |
| CN107114086A (zh) * | 2017-03-31 | 2017-09-01 | 安徽胜华农业发展有限公司 | 一种葛根规范化扦插育苗方法 |
| CN108668655A (zh) * | 2018-03-30 | 2018-10-19 | 湖北省农业科学院中药材研究所 | 一种野葛种苗高效培育方法 |
| WO2019006466A1 (en) * | 2017-06-30 | 2019-01-03 | Booshoot Llc | COMPOSITIONS AND METHODS FOR BIOCULTURE OF LARGE SCALE IN VITRO PLANTS |
| CN110149928A (zh) * | 2019-06-28 | 2019-08-23 | 广西壮族自治区农业科学院 | 一种高效的野葛扦插繁育方法 |
-
2021
- 2021-11-17 CN CN202111362280.7A patent/CN114158368A/zh active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007089568A (ja) * | 2005-08-30 | 2007-04-12 | Mineaki Nishida | プエラリア・ミリフィカの栽培方法 |
| CN106034722A (zh) * | 2016-07-12 | 2016-10-26 | 青岛农业大学 | 一种金色叶植物扦插繁殖的方法 |
| CN107114086A (zh) * | 2017-03-31 | 2017-09-01 | 安徽胜华农业发展有限公司 | 一种葛根规范化扦插育苗方法 |
| WO2019006466A1 (en) * | 2017-06-30 | 2019-01-03 | Booshoot Llc | COMPOSITIONS AND METHODS FOR BIOCULTURE OF LARGE SCALE IN VITRO PLANTS |
| CN108668655A (zh) * | 2018-03-30 | 2018-10-19 | 湖北省农业科学院中药材研究所 | 一种野葛种苗高效培育方法 |
| CN110149928A (zh) * | 2019-06-28 | 2019-08-23 | 广西壮族自治区农业科学院 | 一种高效的野葛扦插繁育方法 |
Non-Patent Citations (1)
| Title |
|---|
| 熊力夫等: "《野葛研究与栽培利用》", 31 May 2007, 湖南科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103975778B (zh) | 一种嫁接橘红种苗方法 | |
| CN103621306A (zh) | 一种通过摘除插穗顶芽的方式提高楸树嫩芽扦插生根率的方法 | |
| CN107223523B (zh) | 一种基于生态位互补的中药材厚朴套种滇黄精的方法 | |
| CN103843546A (zh) | 曼地亚红豆杉扦插快繁栽培方法 | |
| CN105493906A (zh) | 嫁接橘红种苗方法 | |
| CN108811813B (zh) | 一种沙棘嫩芽扦插快繁育苗方法 | |
| Sharma et al. | Direct rooting and hardening of tea microshoots in the field | |
| CN110946064A (zh) | 苔藓伴生铁皮石斛石灰岩面高成活率移栽方法 | |
| CN112753472A (zh) | 一种茶树的扦插育苗方法 | |
| CN104429480A (zh) | 中山杉406硬枝扦插简易繁殖方法 | |
| CN103598021A (zh) | 一种适宜北方生长的香樟树嫁接培育驯化方法 | |
| CN110463455B (zh) | 一种鲁中地区无花果硬枝扦插繁殖育苗的方法 | |
| CN104663191A (zh) | 一种繁育裸花紫珠的方法 | |
| CN106034662A (zh) | 一种白眉海棠的扦插育苗方法 | |
| CN113133374B (zh) | 一种延缓江南牡丹退化的种植方法 | |
| CN114158368A (zh) | 一种野葛嫩枝扦插的方法 | |
| CN108811821B (zh) | 一种促进园林植物绿枝扦插生根的方法 | |
| CN113207570A (zh) | 一种在石漠化地区坡地种植剑麻的方法 | |
| CN105815104A (zh) | 一种提高油用牡丹移栽发枝率的四步法 | |
| CN105028109A (zh) | 一种雪梨扦插育苗方法 | |
| CN112166993A (zh) | 一种太阳能光伏板下玉簪的栽培方法 | |
| CN110558071A (zh) | 一种油橄榄嫩枝扦插育苗的方法 | |
| CN115486363B (zh) | 一种小花老鼠簕叶片水培的育苗方法 | |
| CN104521583B (zh) | 一种蓖麻嫁接方法 | |
| CN112825724B (zh) | 一种杉木幼林套种甜茶的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220311 |