CN114129610B - Method for processing and detoxifying fructus psoraleae based on Lei-Gong method combined with salt moxibustion method - Google Patents
Method for processing and detoxifying fructus psoraleae based on Lei-Gong method combined with salt moxibustion method Download PDFInfo
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Abstract
The invention discloses a processing attenuation method for fructus psoraleae based on a Raynaud method combined with a salt moxibustion method, and belongs to the technical field of traditional Chinese medicine preparation methods. The method comprises the step of preparing the salt fructus psoraleae by a salt moxibustion method of 'Chinese pharmacopoeia' of 2020 edition, and further comprises the following steps: taking fructus Psoraleae, adding 80% medicinal ethanol 5 times the weight of fructus Psoraleae, soaking for 24 hr, pouring out medicinal ethanol, and cleaning with distilled water; then adding 5 times of distilled water, and soaking for 12h; pouring out distilled water, cleaning, steaming in a steamer for 4 hr, taking out, and air drying to obtain the product with reduced toxicity. The invention has the following advantages: the content of toxic components of the fructus psoraleae can be reduced, the content of effective components is kept, and the safety and the effectiveness of the fructus psoraleae in medication are guaranteed; the method has strong practicability, and can promote clinical application and industrial development of fructus Psoraleae and its compound preparation.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine preparation methods, and particularly relates to a method for processing and detoxifying fructus psoraleae based on a Raynaud method combined with a salt moxibustion method.
Background
The fructus psoraleae is dry mature fruit of annual upright herbal fructus psoraleae (Psoralea corilifolia L.) in the leguminosae, is warm in nature, pungent and bitter in taste, enters kidney and spleen channels, has the effects of warming kidney and supporting yang, absorbing qi and relieving asthma, warming spleen and stopping diarrhea, is clinically used for treating kidney-yang deficiency, cold pain of waist and knees, diarrhea before dawn and the like, and is externally used for treating leucoderma and alopecia areata.
However, in recent years, cases that the psoralea corylifolia and the compound preparation thereof are used for a long time or in a large dose to cause serious liver damage are reported in succession, and the psoralea corylifolia and the compound preparation thereof have attracted wide attention of researchers and drug administration departments at home and abroad, which limits the clinical application of the psoralea corylifolia and the compound preparation thereof to a certain extent.
The fructus psoraleae processed product collected in the first part of 2020 edition of Chinese pharmacopoeia is fructus psoraleae processed with salt, and the fructus psoraleae processed with salt not only can guide the medicine downward and supplement liver and kidney, but also can achieve the purpose of reducing toxicity by removing dryness in the medicine. However, the study finds that the fructus psoraleae still has certain hepatotoxicity, and the safety of clinical application of the fructus psoraleae is influenced.
Disclosure of Invention
The Lei Gong method is recorded in Lei Gong processing theory, and is recorded in the ' Lei Gong processing theory ' which says ' when the property and the nature of the dry toxin are caused, the dry toxin is leached with wine for one night and then percolated out, and is leached with eastern running water for three days and three nights and then steamed, and the dry toxin is caused in the day and the day, ancient people have realized that the method can reduce the toxin, and modern researches also prove the effectiveness of the method in reducing the toxin. Because both of these processing methods have the effect of reducing the toxicity, attempts have been made to combine the two methods and to explore the effect of reducing the toxicity of psoralea corylifolia processed after the combination. The salt moxibustion method is recorded according to the pharmacopeia operating method and the Lei's method recorded in Lei's treatise on processing, but the specific contents of the processing technology are recorded little, and basically no objective quantitative index exists. Because the processing conditions of the Leigong method are difficult to control and the quality standard of the processed product is easy to be nonstandard, the research on the processing process of the Leigong method is necessary.
The invention aims to disclose a method for processing and detoxifying fructus psoraleae based on a Lei's method combined with a salt moxibustion method.
The purpose of the invention is realized by the following technical scheme:
a method for processing fructus Psoraleae with reduced toxicity based on Lei's method combined with salt moxibustion comprises preparing fructus Psoraleae with salt moxibustion according to the ' Chinese pharmacopoeia ' 2020 edition, wherein the method further comprises the following steps: taking fructus Psoraleae, adding 80% medicinal ethanol 5 times the weight of fructus Psoraleae, soaking for 24 hr, pouring out medicinal ethanol, and cleaning with distilled water; then adding 5 times of distilled water, and soaking for 12h; pouring out distilled water, cleaning, steaming in a steamer for 4 hr, taking out, and air drying to obtain the final product with reduced toxicity.
The processing attenuation method in the technical scheme comprises the steps of taking 100g of fructus psoraleae medicine, 20mL of saline water containing 2g of salt, moistening for 4h, pouring out the saline water, frying in a frying pan, frying with slow fire until swelling and bursting apart, wherein the surface of the fructus psoraleae medicine is black or black brown, has faint scent and slightly salty taste, and cooling to obtain the fructus psoraleae.
The invention has the following beneficial effects:
1. the processing and toxicity reducing method can reduce the content of toxic components of the fructus psoraleae, simultaneously reserve the content of effective components, and ensure the safety and the effectiveness of the medication of the fructus psoraleae. The method has strong practicability, and can promote clinical application and industrial development of fructus Psoraleae and its compound preparation.
2. The invention passes earlier stage preliminary tests [ single factor test: six factors (alcohol concentration, alcohol-drug ratio, alcohol soaking time, water-drug ratio, water soaking time and steaming time) and a three-factor three-level orthogonal test optimize the obtained processing process, determine the processing process of the Lei's method, and solve the problems that the processing process conditions of the traditional Lei's method are difficult to control and the processed product quality standard is easy to cause.
Description of the drawings:
1. FIG. 1 is an HPLC chart of the negative control solution (A), the mixed control solution (B) and the test solution (C); in figure 1, psoralen; 2. isopsoralen; 3. neopsoralea isoflavone; 4. psoralen A; 5. psoralen B; 6. bakuchiol.
2. FIG. 2 shows the pathological morphology of mouse liver tissue (HE, 200X); arrows indicate central venous distension congestion, inflammatory cell infiltration.
The specific implementation mode is as follows:
in order to facilitate understanding of the technical scheme of the invention, the method for processing and detoxifying fructus psoraleae based on the reich method and the salt moxibustion method is further described in the following specific examples and experimental examples.
The first embodiment is as follows:a method for processing fructus Psoraleae based on Lei's method combined with salt moxibustion comprises:
preparing salt fructus psoraleae according to a salt broiling method of Chinese pharmacopoeia: taking 100g of fructus psoraleae medicinal material, moistening 20mL of saline water containing 2g of salt for 4h, pouring out the saline water, frying in a frying pan, frying with slow fire until swelling and bursting apart, wherein the surface is black or black brown, the smell is fragrant, the taste is slightly salty, and cooling to obtain fructus psoraleae;
taking a proper amount of fructus psoraleae, adding 80% medicinal ethanol which is 5 times of the weight of the fructus psoraleae, soaking for 24 hours, pouring out the medicinal ethanol, and cleaning with distilled water; then adding 5 times of distilled water, and soaking for 12h; pouring out distilled water, cleaning, steaming in a steamer for 4 hr, taking out, and air drying to obtain the final product.
The first test example:processing fructus Psoraleae and evaluating hepatotoxicity based on Lei's method combined with salt moxibustion:
1 experimental materials:
1.1 Instrument:
model LC-20AD high performance liquid chromatography (Shimadzu, japan); BT25S electronic balance (one hundred thousand analytical balance, sydows scientific instruments ltd); KQ5200-B ultrasonic cleaning apparatus (ultrasonic Instrument Co., ltd., kunshan city); TG16-W high speed centrifuge (Changshan appearance centrifuge, inc.); an Epoch microplate reader (berms instruments ltd, usa); an ASP300S type full-automatic closed dehydrator (Leica); model RM2135 microtomes (Leica); BX51 type microscope (OLYMPUS); embedding machines of BMJ-1 type (Tianjin aeronautical electromechanics, inc.); S1-M82 powdering machine (Jiuyang GmbH); a steamer (Zhejiang Supor, ltd.); an induction cooker of C21-RT2149, U.S. (Life electric appliances manufacturing Co., ltd., of Guangdong U.S.); pharmacopeia No. three sieve (shanghai chubai laboratory equipment limited).
1.2 drugs and reagents:
fructus Psoraleae (produced in Henan of origin, purchased from Beijing people-Wei Chinese medicinal decoction pieces Co., ltd., lot number 20110306) was identified as dry mature fruit of Psoralea corylifolia L. of Leguminosae by Yuanhilong, the national center of China's liberation military air force specialty medicine, national center of Yuanhilang, as Yamamai, yuanhong;
psoralen, isopsoralen, neobavachin, psoralen B, and bakuchiol (the batches of the biological technology of Beijing Baiopa Bowei are 20062812, 20061812, 20062205, 20060805,20121110 and 20060912, and the purities are all more than or equal to 98%); ALT and AST kits (Nanjing institute of bioengineering, ltd., lots 20201206 and 20201112, respectively); common salt (medium salt industry, ltd); 4% paraformaldehyde solution (Beijing Soilebao Tech. Co., ltd., batch No. 20200508); 95% medicinal ethanol (Nanjing Runzhi petrochemical Co., ltd.); acetonitrile and methanol (chromatographically pure, fisher corporation); formic acid (chromatographically pure, shanghai Michelin Biotechnology Ltd.); the rest reagents are analytically pure.
1.3 animals:
kunming mouse, male, SPF grade, weight (20 + -2) g, purchased from Beijing Keyu animal cultivation center, and production license number SCXK (Jing) 2018-0010.
2, method and result:
2.1 establishing a quantitative analysis method for index components in fructus psoraleae:
2.1.1 chromatographic conditions:
Diamonsil-C18 column (4.6 mm. Times.250mm, 5 μm); mobile phase acetonitrile (A) -0.1% formic acid water (B), gradient elution, 0-12min, 48% -53% A; 12-30min, 53-70% A; 30-40min, 70-85% A; 40-45min, 85% A; 45-50min, 85% -48% A. The column temperature is 30 ℃; the detection wavelength is 240nm; the flow rate is 1.0mL/min; the amount of the sample was 10. Mu.L.
2.1.2 preparation of control stock:
accurately weighing 15.00mg of psoralen, 5.80mg of isopsoralen, 9.80mg of neopsoralen, 8.00mg of psoralen and 9.00mg of psoralen B in a 10mL volumetric flask, and performing methanol constant volume to prepare 1500 mu g/mL psoralen, 580 mu g/mL isopsoralen, 980 mu g/mL neopsoralen, 800 mu g/mL psoralen and 900 mu g/mL psoralen B reference stock solutions. Accurately weighing 21.80mg of bakuchiol in a 25mL volumetric flask, and fixing the volume with methanol to prepare 872 mu g/mL of reference substance stock solution.
2.1.3 preparation of stock solutions for mixed controls:
precisely measuring 1mL of psoralen, 2mL of isopsoralen, 0.5mL of new psoralen isoflavone, 0.5mL of psoralen A, 0.5mL of psoralen B and 5mL of bakuchiol as reference stock solution in a same 10mL volumetric flask, and carrying out constant volume with methanol to obtain a mixed reference stock solution containing 150 mug/mL of psoralen, 116 mug/mL of isopsoralen, 49 mug/mL of new psoralen isoflavone, 40 mug/mL of psoralen A, 45 mug/mL of psoralen B and 436 mug/mL of bakuchiol.
2.1.4 preparation of test solutions:
pulverizing fructus Psoraleae, sieving with a third sieve, precisely weighing 0.1g of powder, placing in a 25mL volumetric flask, adding 20mL of methanol, ultrasonically extracting for 30min, cooling, adding methanol to scale, shaking, centrifuging at 15000r/min for 10min, collecting supernatant, and filtering with 0.45 μm microporous membrane to obtain sample injection solution.
2.1.5 negative control solutions:
methanol was used as a negative control solution.
2.1.6 specificity:
precisely sucking 10 μ L of each of the negative control solution, the mixed control solution and the sample solution, injecting sample under 2.1.1 chromatography conditions, and recording chromatogram, as shown in figure 1 (figure 1 is HPLC chart of the negative control solution (A), the mixed control solution (B) and the sample solution (C)). As can be seen from figure 1, psoralen, isopsoralen, neopsoralen isoflavone, psoralen A, psoralen B and bakuchiol chromatographic peaks can realize baseline separation, the separation degree is greater than 1.5, the theoretical plate number is not less than 3000 according to psoralen peaks, and the negative control solution has no interference to measurement.
2.1.7 Linear relationship:
precisely measuring 5mL of the mixed reference stock solution in a 10mL volumetric flask, diluting with methanol to a constant volume, diluting step by the same method to prepare a series of mixed reference solutions with concentration gradients, and analyzing 10 μ L of the mixed reference solutions respectively according to chromatographic conditions of 2.1.1. The peak area (Y) was plotted as ordinate and the control concentration (X) as abscissa to prepare a standard curve, which is shown in Table 1. The results show that the linear relationship of the 6 index components is good within a certain concentration range.
Table 1 regression equation, correlation coefficient and linear range of index components
2.1.8 precision:
taking a proper amount of the mixed reference solution with the intermediate concentration under the item 2.1.7, continuously sampling and measuring for 6 times under the chromatographic condition under the item 2.1.1, and recording the peak area. The results show that the RSD of the peak areas of psoralen, isopsoralen, neobavachin, psoralen B and bakuchiol are respectively 0.44%, 0.60%, 0.51%, 1.31%, 1.37% and 0.49% (n = 6), which indicates that the precision of the instrument is good.
2.1.9 stability:
taking 0.1g of fructus Psoraleae powder, preparing sample solution according to the method of item 2.1.4, standing at room temperature for 0, 2, 4, 8, 12, and 24h, measuring by sample injection under chromatographic conditions of item 2.1.1, and recording peak area. The results show that the RSD of the peak areas of psoralen, isopsoralen, neobavachin, psoralen B and bakuchiol are respectively 0.52%, 0.42%, 1.10%, 1.05%, 1.63% and 0.87% (n = 6), which indicates that the test solution is basically stable when placed at room temperature for 24 h.
2.1.10 reproducibility:
taking 0.1g of fructus psoraleae powder, 6 parts in total, preparing a sample solution according to the method under item 2.1.4, carrying out sample injection measurement under the chromatographic condition of item 2.1.1, recording peak areas and calculating the content of each index component according to a standard curve method. The results show that the RSDs of the contents of psoralen, isopsoralen, neobavachin, psoralen B and bakuchiol are 0.81%, 1.02%, 1.70%, 1.60% and 0.69% (n = 6), respectively, which indicates that the method has good repeatability.
2.1.11 sample recovery:
taking 0.1g fructus Psoraleae powder, totally 9 parts, adding reference substances according to 80%, 100%, 120% of index component content, respectively, preparing sample solution according to the method under item 2.1.4, introducing sample, measuring, and calculating sample recovery rate. The results show that the average sample adding and recovery rates of psoralen, isopsoralen, neobavachin, psoralen B and bakuchiol are 103.46%, 105.09%, 92.91%, 96.55%, 100.02% and 99.72% respectively, and the RSD is 0.62%, 1.65%, 1.90%, 1.69%, 1.18% and 1.44% respectively, which indicates that the method has good accuracy.
2.2 the processing technology research of fructus psoraleae:
2.2.1 processing fructus psoraleae with salt:
according to the salt grilling method of the four-part general rule (0213) of the 2020 edition of Chinese pharmacopoeia, 100g of fructus psoraleae medicine is taken, 20mL (containing 2g of salt) of salt water is soaked for 4h, the salt water is poured out, the fructus psoraleae medicine is fried in a medicine frying pan, the fructus psoraleae medicine is fried with slow fire until the fructus psoraleae medicine expands and cracks, the surface is black or black brown, the smell is fragrant, the taste is slightly salty, and the fructus psoraleae medicine is prepared for standby after cooling.
2.2.2 processing fructus Psoraleae with Sal by Ranunculi Ternati method:
taking a proper amount of the common psoralea fruit in the same batch, and processing the common psoralea fruit by a Lei-Gong method. Selecting alcohol concentration (A), alcohol soaking time (B) and steaming time (C) as investigation factors based on pre-experiment, setting 3 levels for each factor, taking the comprehensive weighted score of content reduction rate of bakuchiol, neopsoralea isoflavone, psoralen A, psoralen B, psoralen and isopsoralen as index, and adopting L9 (3) 4 ) The orthogonal test optimizes the processing technology, and the factors and levels are shown in table 2.
TABLE 2 Quadrature test factors and levels
According to L9 (3) 4 ) Preparing fructus Psoraleae processed product by orthogonal table, measuring the content of index component in each processed product by established HPLC method, calculating content decrease rate (decrease rate = fructus Psoraleae corresponding component content-processed product corresponding component content/fructus Psoraleae corresponding component content), and evaluating index component change by comprehensive weighted scoring method. According to the content and toxicity of each component in the psoralea corylifolia, the weight coefficients of psoralen, isopsoralen, neopsoralen isoflavone, psoralen B and bakuchiol are respectively 10%, 20%, 12%, 18% and 30%, and the comprehensive weight score Y = Y1 + 10% + Y2 + 10% + Y3 + 20% + Y4 + 12% + Y5 + 18% + Y6 + 30% (Y1, Y2, Y3, Y4, Y5 and Y6 are the content reduction rates of psoralen, isopsoralen, neopsoralen isoflavone, psoralen A, psoralen B and bakuchiol). The higher the comprehensive weight score Y is, the more the toxic components of the fructus psoraleae are reduced, the better the processed product is and the better the processing technology is. The results of the experiment are shown in Table 3, and the results of the analysis of variance are shown in Table 4.
TABLE 3 orthogonal test design and results
TABLE 4 analysis of variance
As can be seen from the visual analysis in Table 3, the primary and secondary sequence affecting the processing process is A>B>C, table 4 analysis of variance shows that the alcohol concentration and the alcohol leaching time have significant influence on the processing process, and the optimal process is determined as A by combining with the production cost factor 2 B 3 C 1 Namely: the alcohol concentration is 80%, the alcohol soaking time is 24h, and the steaming time is 4h. Therefore, the best processing technology of the fructus psoraleae comprises the following steps: preparing fructus Psoraleae by Chinese pharmacopoeia salt moxibustion method, soaking in 5 times of 80% medicinal ethanol for 24 hr, pouring out medicinal ethanol, washing with distilled water, adding 5 times of distilled water, soaking for 12 hr, pouring out distilled water, washing, steaming in a steamer for 4 hr, taking out, and air drying to obtain fructus Psoraleae processed product.
2.2.3 process verification:
in order to further verify the feasibility and stability of the processing technology, 3 parts of processed products are prepared in parallel. The sample solution is prepared according to the method of item 2.1.4, the content of the index components in the processed product is measured by sample injection under the chromatographic condition of item 2.1.1, and the measurement result is shown in Table 5.
Table 5 verification results
As can be seen from Table 5, compared with Buguzhi, the content of Buguzhi and Isopsoralen in the processed product increases by 15.45% and 5.61%, the content of New Buguzhi isoflavone, buguzhi A, buguzhi B and Buguzhi phenol decreases by 72.23%, 84.38%, 74.75% and 61.87%, and the content of index components in the processed product obtained by parallel experiments has no difference basically, indicating that the processing technology has good reproducibility.
2.3 evaluation of hepatotoxicity:
2.3.1 preparation of test drugs:
taking 40g of the fructus psoraleae processed product prepared in the 2.2.2 items, adding 8 times of water, soaking for 0.5h, extracting for 2 times, each time for 1.5h, combining 2 times of extracting solutions, and concentrating to 100mL to obtain the water extracting solution of the fructus psoraleae processed product. The fructus psoraleae replaces the processed product of fructus psoraleae, and the bone fat supplementing water extract is prepared by the same method.
2.3.2 grouping and dosing:
mice were randomly divided into 7 groups of 7 mice each based on body mass. The control group ig is given distilled water with the same volume, the low (1.5 g/kg), medium (3 g/kg) and high (6 g/kg) dosage groups of the fructus psoraleae processed product, and the low (1.5 g/kg), medium (3 g/kg) and high (6 g/kg) dosage groups of the fructus psoraleae are given once a day for 7 days of continuous ig administration.
2.3.3 serum Biochemical assay:
after the administration of the mouse ig is finished, fasting is carried out without water over night, eyeballs are picked and blood is taken, centrifugation is carried out for 10min at 3500r/min at room temperature, and serum is separated. ALT and AST were determined according to the kit instructions.
2.3.4 liver coefficients and histopathological examination:
the liver of the mouse is picked up by laparotomy, weighed and weighed, and the liver coefficient is calculated. Cutting one mouse liver, fixing in 4% paraformaldehyde solution with fixing solution 5-10 times of tissue volume, fixing for 48 hr, slicing with paraffin, conventional HE staining, and observing tissue pathological changes under light microscope.
TABLE 6 liver coefficients, ALT and AST assay results: (
n＝7)
Compared with the control group, the compound of the formula, * P<0.05, ** P<0.01, *** P<0.001。
the results are shown in Table 6, wherein the ALT levels in the high and middle dose groups were significantly increased compared to the control group ( *** P<0.001， ** P<0.01 In the high, medium and low dose groups of psoralea fruit, AST is significantly increased ( *** P<0.001 Liver coefficient is obviously increased in high and medium dose groups of psoralea fruit: ( *** P<0.001， ** P<0.01 And the fructus psoraleae processed product group has no obvious difference.
The pathological examination result is shown in fig. 2 (fig. 2 is pathological morphology of liver tissue (HE, 200 ×)) and compared with the control group, the psoralea corylifolia high and medium dose groups have obvious liver inflammatory cell infiltration and hyperemia; no obvious abnormal change of liver was observed in the processed product group. The result shows that the liver toxicity of the fructus psoraleae processed product is obviously reduced.
And 3, conclusion:
the content of toxic index components can be obviously reduced by processing the fructus psoraleae based on a Raynaud method and a salt broiling method, and the purpose of processing and reducing toxicity can be achieved by the method as verified by a mouse administration fructus psoraleae hepatotoxicity experiment.
In the experiment, the salt moxibustion method and the Raynaud method are combined for processing and attenuating the malaytea scurfpea fruit for the first time, and HPLC for simultaneously measuring the content of 6 index components in the malaytea scurfpea fruit is established. The result shows that the processing method can obviously reduce the content of toxic components. The result of the hepatotoxicity of mice shows that the fructus psoraleae obtained by the processing method has the same dose and time as the administration of the raw fructus psoraleae, the raw fructus psoraleae shows obvious liver injury, and the processed fructus psoraleae does not have liver injury, which indicates that the processing method can effectively reduce the hepatotoxicity of the raw fructus psoraleae.
The content of psoralen and isopsoralen in the fructus psoraleae processed product is improved compared with that of raw psoralea, and the psoralen and isopsoralen are converted in the processing process, so that the content of psoralen and isopsoralen is kept in an effective dosage range, and other toxic index components are greatly reduced, and the safety and effectiveness of the application of fructus psoraleae are guaranteed. The contents of psoralea corylifolial isoflavone, psoralen and psoralen B in the processed product are all obviously reduced, probably because the psoralea corylifolial isoflavone, psoralen and psoralen B belong to flavanone compounds and are unstable at high temperature, and the ring opening of A ring is degraded, and in the processing process, high-concentration medicinal ethanol is added for long-time soaking, and the content of the medicinal ethanol is reduced after the medicinal ethanol is dissolved in the ethanol. The bakuchiol is an isoprenylphenol terpene compound, is a main component of the volatile oil of the bakuchiol, is easy to oxidize, and is heated to volatilize in the processing process, thereby being the main reason of the content reduction.
The crude psoralea fruit is processed by a salt moxibustion method and then processed by a Raynaud method to finally obtain a psoralea fruit processed product, the experimental test shows that the content of target components psoralen, isopsoralen, neobavachin, psoralen B and bakuchiol in the salt psoralea fruit of the intermediate processed product is respectively 7.824, 10.030, 3.078, 1.502, 2.772 and 47.208mg/g, compared with the crude psoralea fruit, the content of toxic components psoralen and isopsoralen are respectively increased by 117.03 percent and 127.70 percent, which shows that the salt moxibustion method can enhance the effects of warming kidney, supporting yang, tonifying kidney and receiving qi; the contents of toxic components including neopsoralea isoflavone, psoralen B and bakuchiol are respectively reduced by 29.53%, 52.14%, 38.17% and 29.18%, which indicates that the salt moxibustion method has the function of processing attenuation. However, the research proves that the toxicity of the salt fructus psoraleae still exists, so the experiment re-concocts the salt fructus psoraleae by a Lei public method, and the result shows that compared with the raw product, the content of psoralen and isopsoralen in the final concocted product is slightly increased, and the content of new psoralen isoflavone, psoralen A, psoralen B and bakuchiol is greatly reduced, which indicates that the concocting method can ensure the effectiveness and the safety of the fructus psoraleae.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention; meanwhile, any equivalent changes, modifications and evolutions of the above embodiments according to the essential technology of the present invention are still within the scope of the technical solution of the present invention.
Claims (1)
1. A method for processing and detoxifying fructus psoraleae based on a Lei's method combined with a salt moxibustion method is characterized by comprising the following steps:
(1) Taking 100g of fructus psoraleae medicinal material, moistening 20mL of saline water containing 2g of salt for 4h, pouring out the saline water, frying in a medicine frying pot, frying with slow fire until swelling and bursting apart, wherein the surface is black or black brown, the smell is fragrant, the taste is slightly salty, and cooling to obtain fructus psoraleae;
(2) Taking fructus Psoraleae, adding 80% medicinal ethanol 5 times the weight of fructus Psoraleae, soaking for 24 hr, pouring out medicinal ethanol, and cleaning with distilled water; adding 5 times of distilled water, soaking for 12h, pouring out the distilled water and cleaning; steaming in a steamer for 4 hr, taking out, and air drying to obtain the final product.
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