CN114128705B - Perfusate for preserving and perfusing isolated eyeball and use device thereof - Google Patents

Perfusate for preserving and perfusing isolated eyeball and use device thereof Download PDF

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CN114128705B
CN114128705B CN202111308690.3A CN202111308690A CN114128705B CN 114128705 B CN114128705 B CN 114128705B CN 202111308690 A CN202111308690 A CN 202111308690A CN 114128705 B CN114128705 B CN 114128705B
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control unit
central control
eyeball
preset
isolated
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CN114128705A (en
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刘奕志
钟刘学颖
郑永欣
郑颖丰
彭婧怡
甘宇
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Zhongshan Ophthalmic Center
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Zhongshan Ophthalmic Center
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
    • A—HUMAN NECESSITIES
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    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
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    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
    • A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
    • A61B3/13—Ophthalmic microscopes
    • A61B3/135—Slit-lamp microscopes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
    • A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
    • A61B3/14—Arrangements specially adapted for eye photography

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Abstract

The invention relates to a perfusate for preserving and perfusing an isolated eyeball and a using device thereof, wherein the using device comprises a perfusate unit, a perfusate unit and a perfusion unit, wherein the perfusate unit is arranged at the top of a preserving chamber and is used for injecting the perfusate into the preserving chamber; the constant temperature unit is arranged outside the preservation chamber and is used for preserving the heat of the preservation chamber; the storage unit is arranged in the preservation chamber and used for storing the isolated eyeball; the detection unit comprises a first detection mechanism for acquiring the size of an eyeball in vitro, wherein the perfusate comprises polymerized hemoglobin, hydroxyethyl starch, sodium lactate, KCl, ceftriaxone and the like, and the osmotic pressure of the perfusate is 300-320mmol/L. The invention is provided with a central control unit, the central control unit is used for comparing the size of the isolated eyeball obtained by a first detection mechanism with the preset size of the isolated eyeball, and the central control unit adjusts the rotation angle of a support piece by controlling the power parameters of a first power mechanism so as to enable all parameters of the isolated eyeball to accord with the preset standard.

Description

Perfusate for preserving and perfusing isolated eyeball and use device thereof
Technical Field
The invention relates to the field of isolated eyeballs, in particular to a perfusate for preserving and perfusing the isolated eyeballs and a using device thereof.
Background
In the field of artificial cornea development, human eye disease treatment and analysis, human cornea transplantation, veterinary animal ophthalmology science research and the like, the ex-vivo eyeballs of animals need to be used in large quantities, at present, the ophthalmology usually uses a low-temperature humid chamber as a short-time preservation method of the ex-vivo eyeballs, most of the existing methods adopt the traditional preservation method, but the method can cause slight turbidity, fluorescein retention and corneal edema to a certain degree, and is not suitable for the research and application at the present stage.
Therefore, how to alleviate the structural and functional disorders of the isolated eyeball after ischemia and maintain the oxygen partial pressure of the tissue, the cellular activity and the structural integrity is a problem to be solved urgently in the current research.
Disclosure of Invention
Therefore, the invention provides the perfusate for preserving and perfusing the isolated eyeball and the using device thereof, which can solve the technical problem that the isolated eyeball can not be preserved to meet the standard by regulating the perfusate and the using method of the perfusate in real time according to the state of the isolated eyeball.
In order to achieve the above object, the present invention provides a perfusate using device for preserving and perfusing an isolated eyeball, comprising:
the liquid filling unit is arranged at the top of the storage chamber and used for filling the storage chamber with the filling liquid, and comprises a liquid filling pipe, a first electromagnetic valve used for controlling the liquid filling amount and a plurality of liquid filling ports arranged on the liquid filling pipe;
the constant temperature unit is arranged outside the preservation chamber and used for preserving heat of the preservation chamber, and comprises a circulating pipe and a heating mechanism for heating circulating water;
the storage unit is arranged in the storage chamber and used for storing the isolated eyeballs and comprises a storage frame, a plurality of support sheets connected with the storage frame and a first power mechanism for controlling the rotation angles of the support sheets;
the detection unit comprises a first detection mechanism and a second detection mechanism, wherein the first detection mechanism is used for acquiring the size of the in-vitro eyeball;
the central control unit is connected with the liquid filling unit, the constant temperature unit, the storage unit and the detection unit and used for comparing the size of the isolated eyeball obtained by the first detection mechanism with the preset size of the isolated eyeball, and the central control unit adjusts the rotation angle of the support piece by controlling the power parameter of the first power mechanism;
the central control unit obtains the current edema level of the isolated eyeball according to the second detection mechanism and compares the current edema level of the isolated eyeball with a preset level, the central control unit obtains that the current edema level of the isolated eyeball is larger than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with a preset standard, the central control unit obtains that the current edema level of the isolated eyeball is smaller than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber accords with the preset standard, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with the preset standard, the central control unit obtains that the axial length change rate of the current in-vitro eyeball is compared with the preset axial length change rate according to the second detection mechanism, and the central control unit adjusts the heating temperature of the heating mechanism, the rotation angle of the support sheet and the perfusion rate of the perfusion unit so that various parameters of the isolated eyeball accord with the preset standard.
Furthermore, the central control unit presets an isolated eyeball size D, selects a rotation angle of the support sheet according to the comparison between the obtained current isolated eyeball size D and the preset isolated eyeball size, wherein,
when D is less than or equal to D1, the central control unit selects a first preset angle theta 1 as the rotation angle of the supporting sheet;
when D1 is larger than D and smaller than D2, the central control unit selects a second preset angle theta 2 as the rotation angle of the supporting sheet;
when D is larger than or equal to D2, the central control unit selects a third preset angle theta 3 as the rotation angle of the supporting sheet;
the central control unit presets an isolated eyeball size D, sets a first preset isolated eyeball size D1 and a second preset isolated eyeball size D2, presets an angle theta, sets a first preset angle theta 1 and a second preset angle theta 2, and presets a third preset angle theta 3.
Further, the central control unit presets a level B0, the central control unit compares the current in-vitro eyeball edema level B obtained by the second detection mechanism with the preset level, and judges whether the current osmotic pressure of the preservation chamber meets the preset standard or not, wherein,
when B is less than B0, the central control unit judges that the osmotic pressure of the current storage chamber meets the preset standard;
and when B is larger than or equal to B0, the central control unit judges that the osmotic pressure of the current storage chamber does not meet the preset standard.
Further, when the central control unit determines that the current osmotic pressure of the storage chamber does not meet the preset standard, the central control unit compares the acquired current in-vitro eyeball eye axis length G with the preset in-vitro eyeball eye axis length G, and the central control unit adjusts the rotation angle of the support sheet, the perfusion rate of the perfusion unit and the osmotic pressure of the perfusion liquid, wherein,
when G is less than or equal to G1, the central control unit increases the perfusion rate VG to VG1;
when G1 is more than G and less than G2, the filling rate VG of the central control unit is increased to VG2, and the heating T of the heating mechanism is increased to T1;
when G is larger than or equal to G2, the central control unit increases the rotation angle theta i of the support sheet to theta i1, the central control unit increases the osmotic pressure P of the perfusion fluid to P1, and the heating temperature T of the heating mechanism is increased to T2;
the central control unit presets an in-vitro eyeball axis length G, sets a first preset in-vitro eyeball axis length G1 and a second preset in-vitro eyeball axis length G2, wherein i =1,2,3.
Further, the central control unit obtains that the current in-vitro eyeball axis length is less than or equal to a first preset in-vitro eyeball axis length, the central control unit increases the perfusion rate VG to VG1, and VG1= VGx (1 + (G1-G)/G1) is set; the central control unit obtains that the current ex-vivo eyeball axis length is between a first preset ex-vivo eyeball axis length and a second preset ex-vivo eyeball axis length, the central control unit perfusion rate VG is increased to VG2, VG2= VG × (1 + (G-G1) × (G2-G)/(G1 × G2)) is set, the heating T of the heating mechanism is increased to T1, and T1= T × (1 + (G-G1) × (G2-G)/(G1 × G2)) is set.
Furthermore, the central control unit acquires that the length of the current in-vitro eyeball axis is greater than or equal to the length of a second preset in-vitro eyeball axis, and rotates the support piece by an angleθ i is increased to θ i1, θ i1= θ i x (1 + (G-G2)/G2) is set, the central control unit increases the perfusate osmotic pressure P to P1, P1= P x (1 + (G-G2) is set 2 G2), the heating temperature T of the heating means is increased to T2, and T2= T × (1 + (G-G2)/G2) is set.
Further, the central control unit presets a perfusion rate standard value VG0, and adjusts the liquid outlet amount of the perfusate according to the comparison between the adjusted perfusion rate and the preset perfusion rate standard value, wherein,
when VGj is not more than VG0, the central control unit reduces the perfusate effluent QC to QC1, and QC1= QC x (1- (VG 0-VGj)/VG 0) is set;
when VGj is larger than VG0, the central control unit increases the perfusate effluent QC to QC2, and sets QC2= = QC × (1- (VGj-VG 0)/VG 0);
wherein j =1,2.
Furthermore, the central control unit presets a standard value theta 0 of the rotation angle of the support sheet, compares the rotation angle of the support sheet after adjustment with the preset standard value, and adjusts the power parameters of the first power mechanism, wherein,
when the theta i1 is larger than or equal to the theta 0, the central control unit increases the power parameter F1 of the first power mechanism to F11, and sets F11= F1 x (1 + (theta i 1-theta 0) 2 /θ0)；
When θ i1 < θ 0, the central control unit reduces the first power mechanism power parameter F1 to F12, and sets F12= F1 × (1- (θ 0- θ i 1) 2 /θ0)。
Furthermore, a first temperature detector is arranged at a water inlet of a circulating pipe in the constant temperature unit, a second temperature detector is arranged at a water inlet of the circulating pipe, the central control unit adjusts the heating temperature of the heating mechanism according to the comparison between the temperature difference delta t obtained by the second temperature detector and the first temperature detector and the preset temperature difference, wherein,
when Δ t is less than or equal to t0, the central control unit increases the heating temperature of the heating mechanism to Tk1, and sets Tk1= Tk x (1 + (t 0- Δ t)/t 0);
when Δ t is less than or equal to t0, the central control unit reduces the heating temperature of the heating mechanism to Tk2 setting Tk2= Tk x (1- (. DELTA.t-t 0)/t 0);
wherein k =1,2.
Further, the perfusate for preserving and perfusing the isolated eyeball contains 35.20g of Poly hemoglobin (Poly-HB), 8.4g of hydroxyethyl starch, 4.1g of sodium lactate, 0.13g of KCl and CaCl per liter 2 0.08g, 0.28mg of 2-methylene bio-cable, 10.14mg of Neostatin, 1.70mg of minocycline, 0.04mg of Q-VD-Oph, 31.43mg of sodium pyruvate, 90.38mg of NXY-0590, 40mg of sodium 3-hydroxybutyrate, 0.025mg of Flunarizene, 1.70mg of glutathione, 20mg of N-acetylcysteine, 1.14mg of lamotrigine, 0.05mg of MMP-9 inhibitor and 76mg of ceftriaxone, and the osmotic pressure of the perfusate is 300-320mmol/L.
Compared with the prior art, the device has the advantages that the device is provided with the central control unit, the central control unit is used for obtaining the size of the isolated eyeball according to the first detection mechanism and comparing the size with the preset size of the isolated eyeball, the central control unit adjusts the rotation angle of the supporting sheet by controlling the power parameter of the first power mechanism, the central control unit obtains the current edema level of the isolated eyeball according to the second detection mechanism and compares the current edema level with the preset level, the central control unit obtains that the current edema level of the isolated eyeball is larger than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with the preset standard, the central control unit obtains that the current edema level of the isolated eyeball is smaller than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber accords with the preset standard, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with the preset standard, the central control unit obtains that the axial length change rate of the current isolated eyeball is compared with the preset axial length rate according to the second detection mechanism, and the central control unit adjusts the heating temperature of the heating mechanism, the rotation angle of the supporting sheet and the perfusion rate of the isolated eyeball so as to enable the parameters to accord with each preset standard.
Particularly, the control unit of the invention is internally provided with the size of the isolated eyeball and divides the preset size of the isolated eyeball into two definite standards, the central control unit acquires the size of the current isolated eyeball according to the first detection mechanism, compares the size of the current isolated eyeball with the preset size of the isolated eyeball and selects the optimal angle as the rotation angle of the support sheet so as to enable the support sheet of the storage unit to support the isolated eyeball, wherein if the size of the current isolated eyeball acquired by the central control unit is smaller than or equal to the first preset size of the isolated eyeball, the central control unit selects the first preset angle and supports the isolated eyeball with a larger rotation angle of the support sheet, if the size of the current isolated eyeball acquired by the central control unit is between the first preset size of the isolated eyeball and the second preset size of the isolated eyeball, the central control unit selects the second preset angle, supports the isolated eyeball with a middle rotation angle of the support sheet with a middle value, and if the size of the isolated eyeball acquired by the central control unit is larger than or equal to the second preset size of the isolated eyeball, the central control unit selects the third preset angle, supports the isolated eyeball with a smaller rotation angle, and avoids the isolated eyeball from moving caused by the support sheet or the isolated eyeball moving of the isolated eyeball with the smaller rotation angle.
Particularly, the invention is provided with a preset level, the central control unit compares the current edema level of the isolated eyeball obtained by the second detection mechanism with the preset level, and judges whether the osmotic pressure in the preservation chamber meets the preset standard, wherein if the current edema level of the isolated eyeball obtained by the central control unit is smaller than the preset level, the current osmotic pressure of the preservation chamber does not generate edema, and if the current edema level of the isolated eyeball obtained by the central control unit is larger than or equal to the preset level, the current edema level of the isolated eyeball is generated, and the current osmotic pressure of the preservation chamber does not meet the preset standard.
In particular, the invention sets a central control unit for determining that the osmotic pressure in the current preserving chamber does not meet a preset standard, the central control unit compares the current ocular axis length of the isolated eyeball obtained by the second detection mechanism with the preset ocular axis length of the isolated eyeball, determines the influence degree of the edema of the isolated eyeball on the isolated eyeball, and further increases the osmotic pressure in the preserving chamber according to different degrees to reduce the edema level of the isolated eyeball, wherein if the ocular axis length of the isolated eyeball is less than or equal to the ocular axis length of the first preset isolated eyeball, the influence of the current osmotic pressure on the isolated eyeball is not large, the central control unit increases the fluidity of the perfusate in the preserving chamber by properly increasing the perfusion rate, keeps the wettability of the isolated eyeball, avoids the edema of the isolated eyeball from increasing, if the ocular axis length of the isolated eyeball is between the ocular axis length of the first preset isolated eyeball and the ocular axis length of the second preset isolated eyeball, the central control unit is used for improving the temperature of the storage chamber by improving the heating temperature of the heating mechanism to improve the osmotic pressure in the storage chamber so as to reduce the edema of the isolated eyeball, if the eye axis length of the isolated eyeball is larger than or equal to the second preset eye axis length of the isolated eyeball, the current osmotic pressure has larger influence on the isolated eyeball, the central control unit is used for improving the osmotic pressure of the storage chamber by filling the high osmotic pressure perfusion fluid and improving the heating temperature of the heating mechanism so as to quickly reduce the edema of the isolated eyeball and avoid causing more serious damage to the isolated eyeball, and meanwhile, in order to keep the clamping stability of the isolated eyeball, the central control unit is used for improving the rotation angle so as to avoid the change of the eye axis, the in vitro eyeball is damaged due to the mismatching of the rotation angle of the support piece.
Particularly, the invention is provided with a perfusion rate standard value, the central control unit compares the regulated perfusion rate with a preset standard value, and regulates the liquid outlet amount of the perfusate so as to enable the circulation of the perfusate in the preservation chamber to meet the preset standard, wherein if the regulated perfusion rate is less than or equal to the preset standard value, the central control unit reduces the liquid outlet amount of the perfusate, and if the regulated perfusion rate is greater than the preset standard value, the central control unit increases the liquid outlet amount of the perfusate so as to keep the osmotic pressure of the perfusate in the preservation chamber and simultaneously avoid the damage to the isolated eyeball caused by too much or too little perfusate in the preservation chamber.
Particularly, the power mechanism is provided with a standard value of the rotation angle of the supporting sheet, and the central control unit compares the rotation angle of the supporting sheet after adjustment with a preset standard value and adjusts the power parameter of the first power mechanism for controlling the rotation angle of the supporting sheet, wherein if the rotation angle of the supporting sheet after adjustment is larger than or equal to the preset standard value, the central control unit judges that the power parameter of the first power mechanism is increased so as to increase the rotation angle of the current supporting sheet, and if the rotation angle of the supporting sheet after adjustment is smaller than the preset standard value, the central control unit judges that the power parameter of the first power mechanism is decreased so as to decrease the rotation angle of the current supporting sheet.
Particularly, the temperature difference between the water inlet and the water outlet of the circulating pipe of the constant temperature unit is compared with a preset value, the heating temperature of the heating mechanism is adjusted, so that the temperature in the preservation chamber is kept, and the problem that the circulating water temperature is not constant due to excessive heat dissipation is avoided.
In particular, the perfusion fluid provided by the invention is an isotonic solution, which contains various ions and colloid components, can maintain the balance of the osmotic pressure inside and outside blood vessels and inside and outside cells, and contains an oxygenation component Poly-HB for maintaining the oxygen partial pressure of tissues. Meanwhile, the perfusate comprises a component for protecting retinal ganglion cells, a component for inhibiting apoptosis and necrosis, a component for inhibiting a calcium channel and a component for protecting nerves, combines the special structural and functional characteristics of eyes, can reduce ischemia and reperfusion injury of an isolated eyeball to the maximum extent, and preserves the structural and functional integrity of the eyeball, is used for relieving structural and functional disorders of organs after ischemia, and maintains the oxygen partial pressure of tissues, the activity of cells and the structural integrity.
Drawings
FIG. 1 is a schematic structural diagram of a device for using a perfusate for preserving and perfusing an isolated eyeball according to an embodiment of the invention;
FIG. 2 is a top view of a storage unit according to an embodiment of the invention.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described below with reference to examples; it should be understood that the specific embodiments described herein are merely illustrative of the invention and do not delimit the invention.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and do not limit the scope of the present invention.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Please refer to fig. 1, which is a schematic structural diagram of a device for using a perfusion solution for storing and perfusing an isolated eyeball according to an embodiment of the present invention, including a perfusion unit disposed at the top of a storage chamber and used for injecting the perfusion solution into the storage chamber 8, where the perfusion unit includes a perfusion tube 7, a first solenoid valve 5 used for controlling a perfusion rate of the perfusion solution, and a plurality of perfusion ports 6 disposed on the perfusion tube, and the perfusion solution is injected into the storage chamber through the perfusion ports on the perfusion tube to moisten the current isolated eyeball; the constant temperature unit is arranged outside the storage chamber and used for preserving heat of the storage chamber, and comprises a circulating pipe 10 and a heating mechanism 11 for heating circulating water, wherein a first temperature detector 16 is arranged at a water inlet 14 of the circulating pipe and used for acquiring the temperature of a water inlet of the circulating pipe, a second temperature detector 13 is arranged at a water outlet 12 of the circulating pipe and used for acquiring the temperature of a water outlet of the circulating pipe, a second electromagnetic valve 17 is arranged on the circulating pipe and used for controlling the water injection amount into the circulating pipe, and a water delivery pump 9 is further arranged on the circulating pipe and used for providing power for the water circulation in the circulating pipe; the storage unit is arranged in the storage chamber and used for storing the in-vitro eyeballs, and comprises a storage frame 2, a plurality of support sheets 4 connected with the storage frame and a first power mechanism 1 for controlling the rotation angles of the support sheets, wherein the first power mechanism is connected with each support sheet through a plurality of connecting shafts 3; a perfusate outlet is arranged at the bottom of the storage chamber, a third electromagnetic valve 15 is arranged at the outlet and is used for controlling the liquid outlet rate of the perfusate; the detection unit comprises a first detection mechanism and a second detection mechanism, wherein the first detection mechanism is used for acquiring the size of the in-vitro eyeball, and the second detection mechanism is used for acquiring the edema level and the ocular axis length of the in-vitro eyeball; the central control unit is connected with the liquid filling unit, the constant temperature unit, the storage unit and the detection unit and used for comparing the size of the isolated eyeball obtained by the first detection mechanism with the preset size of the isolated eyeball, and the central control unit adjusts the rotation angle of the support piece by controlling the power parameter of the first power mechanism;
the central control unit obtains the current edema level of the isolated eyeball according to the second detection mechanism and compares the current edema level of the isolated eyeball with a preset level, the central control unit obtains that the current edema level of the isolated eyeball is larger than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with a preset standard, the central control unit obtains that the current edema level of the isolated eyeball is smaller than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber accords with the preset standard, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with the preset standard, the central control unit obtains that the axial length change rate of the current in-vitro eyeball is compared with the preset axial length change rate according to the second detection mechanism, and the central control unit adjusts the heating temperature of the heating mechanism, the rotation angle of the support sheet and the perfusion rate of the perfusion unit so that various parameters of the isolated eyeball accord with the preset standard.
Please refer to fig. 2, which is a top view of a storage unit according to an embodiment of the present invention, including a storage rack 2 and a plurality of support plates 4 connected to the storage rack, it can be understood by those skilled in the art that the shape, structure and arrangement of the support plates are not limited in the embodiment of the present invention as long as the support plates can support an ex-vivo eyeball, wherein the support plates are preferably made of a flexible material to avoid damaging the ex-vivo eyeball.
The central control unit presets an isolated eyeball size D, selects a rotation angle of the support sheet according to the comparison between the acquired current isolated eyeball size D and the preset isolated eyeball size, wherein,
when D is less than or equal to D1, the central control unit selects a first preset angle theta 1 as the rotation angle of the supporting sheet;
when D1 is larger than D and smaller than D2, the central control unit selects a second preset angle theta 2 as the rotation angle of the supporting sheet;
when D is larger than or equal to D2, the central control unit selects a third preset angle theta 3 as the rotation angle of the support sheet;
the central control unit presets an isolated eyeball size D, sets a first preset isolated eyeball size D1 and a second preset isolated eyeball size D2, presets an angle theta, sets a first preset angle theta 1 and a second preset angle theta 2, and presets a third preset angle theta 3.
Specifically, the control unit of the invention is internally provided with an isolated eyeball size, and divides the preset isolated eyeball size into two definite standards, the central control unit acquires the current isolated eyeball size according to the first detection mechanism, compares the current isolated eyeball size with the preset isolated eyeball size, and selects the optimal angle as the rotation angle of the support sheet so as to enable the support sheet of the storage unit to support the isolated eyeball, wherein if the current isolated eyeball size acquired by the central control unit is smaller than or equal to the first preset isolated eyeball size, the central control unit selects the first preset angle to support the isolated eyeball with a smaller size by using a larger rotation angle of the support sheet, if the current isolated eyeball size acquired by the central control unit is between the first preset isolated eyeball size and the second preset isolated eyeball size, the central control unit selects the second preset angle to support the isolated eyeball with a middle rotation angle of the support sheet with a middle value, and if the current isolated eyeball size acquired by the central control unit is larger than or equal to the second preset isolated eyeball size, the central control unit selects the third preset angle, and supports the isolated eyeball with a smaller rotation angle to avoid the isolated eyeball size from being matched with the smaller support sheet rotation angle, or avoid moving of the isolated eyeball not being damaged.
Specifically, the embodiment of the present invention provides a preferable implementation scheme, in which the front-back diameter of the isolated eyeball is used as an evaluation mode, and the rotation angle of the support piece is adjusted to fix the isolated eyeball, wherein the preferable parameter of the front-back diameter of the isolated eyeball is preset to be 20-30mm.
Wherein the central control unit presets a level B0, compares the current in-vitro eyeball edema level B acquired by the second detection mechanism with the preset level, and judges whether the current osmotic pressure of the preservation chamber meets the preset standard, wherein,
when B is less than B0, the central control unit judges that the osmotic pressure of the current storage chamber meets a preset standard;
and when B is larger than or equal to B0, the central control unit judges that the osmotic pressure of the current storage chamber does not meet the preset standard.
Specifically, the preset level is set, the central control unit compares the current in-vitro eyeball edema level obtained through the second detection mechanism with the preset level, and judges whether the osmotic pressure in the preservation chamber meets the preset standard or not, wherein if the current in-vitro eyeball edema level obtained through the central control unit is smaller than the preset level, the fact that edema does not occur in the current in-vitro eyeball is indicated, the current osmotic pressure in the preservation chamber meets the preset standard, and if the current in-vitro eyeball edema level obtained through the central control unit is larger than or equal to the preset level, the fact that edema occurs in the current in-vitro eyeball is indicated, and the current osmotic pressure in the preservation chamber does not meet the preset standard.
Specifically, the embodiment of the invention does not limit the type, the setting mode, the type and the like of the second detection mechanism as long as the second detection mechanism can acquire whether the in vitro eyeball generates edema, the second detection mechanism can be a slit lamp or an eyeball detection machine and the like, and meanwhile, the embodiment of the invention does not limit the method for acquiring the grade of the edema of the in vitro eyeball as long as the method can judge whether the in vitro eyeball generates edema, and the embodiment of the invention provides a preferable setting method, wherein the grade of the edema of the in vitro eyeball is divided into four grades, and the grade of the edema of the in vitro eyeball is set, so that the limited atomized edema of corneal epithelium can be seen, the corneal endothelium is smooth, and the iris texture is clear and visible; the second grade is cornea light grey edema, endothelium roughness and iris texture blurring; the third level is diffuse grey-white edema of the cornea, the corneal endothelium is changed in a cracking way, and the iris texture is not clear; the fourth stage is opalescent edema of the cornea, and the intraocular structure is not clear.
When the central control unit judges that the current osmotic pressure of the storage chamber does not meet the preset standard, the central control unit compares the obtained current in-vitro eyeball eye axis length G with the preset in-vitro eyeball eye axis length G, and adjusts the rotation angle of the support sheet, the perfusion rate of the perfusion unit and the osmotic pressure of perfusion liquid, wherein,
when G is less than or equal to G1, the central control unit increases the perfusion rate VG to VG1;
when G1 is more than G and less than G2, the filling rate VG of the central control unit is increased to VG2, and the heating T of the heating mechanism is increased to T1;
when G is larger than or equal to G2, the central control unit increases the rotation angle theta i of the support sheet to theta i1, the central control unit increases the osmotic pressure P of the perfusion fluid to P1, and the heating temperature T of the heating mechanism is increased to T2;
the central control unit presets an in-vitro eyeball axis length G, sets a first preset in-vitro eyeball axis length G1 and a second preset in-vitro eyeball axis length G2, wherein i =1,2,3.
Specifically, the central control unit acquires that the current in-vitro eyeball axis length is less than or equal to a first preset in-vitro eyeball axis length, the central control unit increases the perfusion rate VG to VG1, and VG1= VG × (1 + (G1-G)/G1) is set.
Wherein the central control unit acquires that the current ex vivo ocular axial length is between a first preset ex vivo ocular axial length and a second preset ex vivo ocular axial length, the central control unit perfusion rate VG is increased to VG2, VG2= VG × (1 + (G-G1) × (G2-G)/(G1 × G2)) is set, the heating T of the heating mechanism is increased to T1, and T1= T × (1 + (G-G1) × (G2-G)/(G1 × G2)) is set.
Specifically, the central control unit obtains the length of the current in-vitro eyeball axis greater than or equal to the length of a second preset in-vitro eyeball axis, the central control unit increases the rotation angle θ i of the support sheet to θ i1, sets θ i1= θ i x (1 + (G-G2)/G2), the central control unit increases the osmotic pressure P of the perfusion fluid to P1, and sets P1= P x (1 + (G-G2) 2 G2), the heating temperature T of the heating means is increased to T2, and T2= T × (1 + (G-G2)/G2) is set.
Specifically, the invention sets a central control unit for determining that the osmotic pressure in the current preserving chamber does not meet a preset standard, the central control unit compares the current ocular axis length of the isolated eyeball obtained by a second detection mechanism with the preset ocular axis length of the isolated eyeball, determines the influence degree of the edema of the isolated eyeball on the isolated eyeball, and further increases the osmotic pressure in the preserving chamber according to different degrees to reduce the edema level of the isolated eyeball, wherein if the ocular axis length of the isolated eyeball is less than or equal to the ocular axis length of the first preset isolated eyeball, the influence of the current osmotic pressure on the isolated eyeball is small, the central control unit increases the fluidity of the perfusate in the preserving chamber by properly increasing the perfusion rate, keeps the wettability of the isolated eyeball, avoids the edema condition of the isolated eyeball from increasing, if the ocular axis length of the isolated eyeball is between the ocular axis length of the first preset isolated eyeball and the ocular axis length of the second preset isolated eyeball, the central control unit is used for filling a high-osmotic-pressure perfusion solution, increasing the heating temperature of the heating mechanism to increase the osmotic pressure in the preservation chamber so as to reduce the edema of the isolated eyeball, and increasing the temperature of the preservation chamber so as to increase the osmotic pressure of the perfusion solution if the length of the eye axis of the isolated eyeball is greater than or equal to the length of the eye axis of a second preset isolated eyeball so as to quickly reduce the edema of the isolated eyeball and avoid causing more serious damage to the isolated eyeball, and simultaneously, in order to keep the clamping stability of the isolated eyeball, the central control unit is used for increasing the rotation angle and avoiding the change of the eye axis, the in vitro eyeball is damaged due to the mismatching of the rotation angle of the support piece.
The central control unit presets a perfusion rate standard value VG0, and adjusts the liquid outlet amount of the perfusate according to the comparison between the adjusted perfusion rate and the preset perfusion rate standard value, wherein,
when VGj is not more than VG0, the central control unit reduces the perfusate outflow QC to QC1, and QC1= QC × (1- (VG 0-VGj)/VG 0) is set;
when VGj is larger than VG0, the central control unit increases the perfusate outflow QC to QC2, and sets QC2= = QC × (1- (VGj-VG 0)/VG 0);
wherein j =1,2.
Specifically, the invention is provided with a perfusion rate standard value, the central control unit compares the regulated perfusion rate with a preset standard value, and regulates the liquid outlet amount of the perfusate so as to enable the circulation of the perfusate in the preservation chamber to meet the preset standard, wherein if the regulated perfusion rate is less than or equal to the preset standard value, the central control unit reduces the liquid outlet amount of the perfusate, and if the regulated perfusion rate is greater than the preset standard value, the central control unit increases the liquid outlet amount of the perfusate so as to keep the osmotic pressure of the perfusate in the preservation chamber, and simultaneously avoid the damage to the isolated eyeball caused by too much or too little perfusate in the preservation chamber.
Wherein, the central control unit presets a standard value theta 0 of the rotation angle of the support sheet, compares the adjusted rotation angle of the support sheet with the preset standard value and adjusts the power parameter of the first power mechanism, wherein,
when the theta i1 is larger than or equal to the theta 0, the central control unit increases the power parameter F1 of the first power mechanism to F11, and sets F11= F1 x (1 + (theta i 1-theta 0) 2 /θ0)；
When θ i1 < θ 0, the central control unit reduces the first power mechanism power parameter F1 to F12, and sets F12= F1 × (1- (θ 0- θ i 1) 2 /θ0)。
Specifically, the power parameter of the first power mechanism for controlling the rotation angle of the support sheet is adjusted by the central control unit according to the comparison between the adjusted rotation angle of the support sheet and a preset standard value, wherein if the adjusted rotation angle of the support sheet is larger than or equal to the preset standard value, the central control unit judges that the power parameter of the first power mechanism is increased so as to increase the current rotation angle of the support sheet, and if the adjusted rotation angle of the support sheet is smaller than the preset standard value, the central control unit judges that the power parameter of the first power mechanism is decreased so as to decrease the current rotation angle of the support sheet.
Wherein, a first temperature detector is arranged at the water inlet of the circulating pipe in the constant temperature unit, a second temperature detector is arranged at the water inlet of the circulating pipe, the central control unit adjusts the heating temperature of the heating mechanism according to the comparison between the temperature difference Deltat obtained by the second temperature detector and the first temperature detector and the preset temperature difference, wherein,
when Δ t is less than or equal to t0, the central control unit increases the heating temperature of the heating mechanism to Tk1, and sets Tk1= Tk x (1 + (t 0- Δ t)/t 0);
when Δ t is less than or equal to t0, the central control unit reduces the heating temperature of the heating mechanism to Tk2 setting Tk2= Tk x (1- (. DELTA.t-t 0)/t 0);
wherein k =1,2.
Specifically, the temperature difference between the water inlet and the water outlet of the circulating pipe of the constant temperature unit is compared with a preset value, the heating temperature of the heating mechanism is adjusted to keep the temperature in the storage chamber, and the problem that the circulating water temperature is not constant due to excessive heat dissipation is avoided.
The perfusate for preserving and perfusing isolated eyeball contains Poly hemoglobin (Poly-HB) 35.20g, hydroxyethyl starch 8.4g, sodium lactate 4.1g, kcl0.13g and CaCl per liter 2 0.08g, 0.28mg of 2-methylene biogel, 10.14mg of Neostatin, 1.70mg of minocycline, 0.04mg of Q-VD-Oph, 31.43mg of sodium pyruvate, 90.38mg of NXY-0590, 40mg of sodium 3-hydroxybutyrate, 0.025mg of Flunarinie, 1.70mg of glutathione, 20mg of N-acetylcysteine, 1.14mg of lamotrigine, 0.05mg of MMP-9 inhibitor and 76mg of ceftriaxone, and the osmotic pressure of the perfusate is 300-320mmol/L.
Specifically, the perfusate provided by the present invention is an isotonic solution containing various ions and colloidal components capable of maintaining the balance of the osmotic pressure inside and outside the blood vessel and inside and outside the cell, and an oxygenation component Poly-HB for maintaining the tissue oxygen partial pressure. Meanwhile, the perfusate comprises components for protecting retinal ganglion cells, components for inhibiting apoptosis and necrosis, components for inhibiting calcium channels and components for protecting nerves, combines the special structural and functional characteristics of eyes, can reduce ischemia and reperfusion injury of isolated eyeballs to the maximum extent, and preserves the structural and functional integrity of the eyeballs, is used for relieving structural and functional disorders of organs after ischemia, and maintains the oxygen partial pressure of tissues, the activity of cells and the structural integrity.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is apparent to those skilled in the art that the scope of the present invention is not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.

Claims (8)

1. A use device of a perfusate for preserving and perfusing an isolated eyeball, comprising:
the liquid filling unit is arranged at the top of the storage chamber and used for filling the storage chamber with a filling liquid, and comprises a liquid filling pipe, a first electromagnetic valve used for controlling the liquid filling amount and a plurality of liquid filling ports arranged on the liquid filling pipe;
the constant temperature unit is arranged outside the preservation chamber and used for preserving heat of the preservation chamber, and comprises a circulating pipe and a heating mechanism for heating circulating water;
the storage unit is arranged in the storage chamber and used for storing the in-vitro eyeballs, and comprises a storage frame, a plurality of support sheets connected with the storage frame and a first power mechanism for controlling the rotation angles of the support sheets;
the detection unit comprises a first detection mechanism and a second detection mechanism, wherein the first detection mechanism is used for acquiring the size of the in-vitro eyeball;
the central control unit is connected with the liquid filling unit, the constant temperature unit, the storage unit and the detection unit and used for comparing the size of the isolated eyeball obtained by the first detection mechanism with the preset size of the isolated eyeball, and the central control unit adjusts the rotation angle of the support piece by controlling the power parameter of the first power mechanism;
the central control unit acquires the current edema level of the isolated eyeball according to the second detection mechanism and compares the current edema level of the isolated eyeball with a preset level, the central control unit acquires that the current edema level of the isolated eyeball is larger than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with a preset standard, the central control unit acquires that the current edema level of the isolated eyeball is smaller than the preset level, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber accords with the preset standard, the central control unit judges that the osmotic pressure of the filling liquid in the preservation chamber does not accord with the preset standard, the central control unit acquires that the current axial length change rate of the isolated eyeball and compares the preset axial length change rate of the isolated eyeball according to the second detection mechanism, and the central control unit adjusts the heating temperature of the heating mechanism, the rotation angle of the support sheet and the perfusion rate of the filling liquid unit so as to enable various parameters of the isolated eyeball to accord with the preset standard;
when the central control unit judges that the current osmotic pressure of the storage chamber does not meet the preset standard, the central control unit compares the obtained current in-vitro eyeball eye axis length G with the preset in-vitro eyeball eye axis length G, and adjusts the rotation angle of the support sheet, the perfusion rate of the perfusion unit and the osmotic pressure of the perfusion liquid, wherein,
when G is less than or equal to G1, the central control unit increases the perfusion rate VG to VG1;
when G1 is more than G and less than G2, the filling rate VG of the central control unit is increased to VG2, and the heating T of the heating mechanism is increased to T1;
when G is larger than or equal to G2, the central control unit increases the rotation angle theta i of the support sheet to theta i1, the central control unit increases the osmotic pressure P of the perfusion fluid to P1, and the heating temperature T of the heating mechanism is increased to T2;
the central control unit presets an in-vitro eyeball axis length G, sets a first preset in-vitro eyeball axis length G1 and a second preset in-vitro eyeball axis length G2, wherein i =1,2,3.
2. The use device of the perfusion fluid for preserving and perfusing the isolated eyeball as claimed in claim 1, wherein the central control unit presets an isolated eyeball size D, the central control unit selects a rotation angle of the support sheet according to the comparison between the obtained current isolated eyeball size D and the preset isolated eyeball size, wherein,
when D is less than or equal to D1, the central control unit selects a first preset angle theta 1 as the rotation angle of the supporting sheet;
when D1 is larger than D and smaller than D2, the central control unit selects a second preset angle theta 2 as the rotation angle of the support sheet;
when D is larger than or equal to D2, the central control unit selects a third preset angle theta 3 as the rotation angle of the supporting sheet;
the central control unit presets an isolated eyeball size D, sets a first preset isolated eyeball size D1 and a second preset isolated eyeball size D2, presets an angle theta, sets a first preset angle theta 1 and a second preset angle theta 2, and presets a third preset angle theta 3.
3. The apparatus for using perfusion fluid for preserving and perfusing an ex-vivo eyeball as claimed in claim 2, wherein the central control unit is configured to preset a level B0, and the central control unit determines whether the osmotic pressure of the current preserving chamber meets a preset standard according to the comparison between the current edema level B of the ex-vivo eyeball obtained by the second detection mechanism and the preset level, wherein,
when B is less than B0, the central control unit judges that the osmotic pressure of the current storage chamber meets the preset standard;
and when B is larger than or equal to B0, the central control unit judges that the osmotic pressure of the current storage chamber does not meet the preset standard.
4. The use device of the perfusion fluid for preserving and perfusing the isolated eyeball according to claim 3, wherein the central control unit obtains the current isolated eyeball axis length less than or equal to a first preset isolated eyeball axis length, the central control unit increases the perfusion rate VG to VG1, and the setting VG1= VGx (1 + (G1-G)/G1); the central control unit obtains that the current ex-vivo eyeball axis length is between a first preset ex-vivo eyeball axis length and a second preset ex-vivo eyeball axis length, the central control unit perfusion rate VG is increased to VG2, VG2= VG × (1 + (G-G1) × (G2-G)/(G1 × G2)) is set, the heating T of the heating mechanism is increased to T1, and T1= T × (1 + (G-G1) × (G2-G)/(G1 × G2)) is set.
5. The use device of the perfusate for preserving and perfusing the isolated eyeball as set forth in claim 4, wherein the central control unit obtains the current length of the axis of the isolated eyeball greater than or equal to the second preset length of the axis of the isolated eyeball, the central control unit increases the rotation angle θ i of the support plate to θ i1, and sets θ i1= θ i x (1 + (G-G2)/G2), and the central control unit increases the osmotic pressure P of the perfusate to P1, and sets P1= P x (1 + (G-G2) 2 G2), the heating temperature T of the heating mechanism is increased to T2, and T2= T × (1 + (G-G2)/G2) is set.
6. The apparatus for using perfusion fluid for preserving and perfusing an ex-vivo eyeball as claimed in claim 4, wherein the central control unit is used for presetting a perfusion rate standard value VG0, and adjusting the outflow volume of the perfusion fluid according to the comparison between the regulated perfusion rate and the preset perfusion rate standard value, wherein,
when VGj is not more than VG0, the central control unit reduces the perfusate effluent QC to QC1, and QC1= QC x (1- (VG 0-VGj)/VG 0) is set;
when VGj is larger than VG0, the central control unit increases the perfusate effluent QC to QC2, and sets QC2= = QC × (1- (VGj-VG 0)/VG 0);
wherein j =1,2.
7. The use device of the perfusion solution for preserving and perfusing the isolated eyeball according to claim 4, wherein the central control unit presets a standard value θ 0 of the rotation angle of the support sheet, compares the rotation angle of the support sheet after adjustment with the preset standard value, and adjusts the power parameters of the first power mechanism, wherein,
when the theta i1 is larger than or equal to the theta 0, the central control unit increases the power parameter F1 of the first power mechanism to F11, and sets F11= F1 x (1 + (theta i 1-theta 0) 2 /θ0）；
When the theta 1 is less than the theta 0, the central control unit reduces the power parameter F1 of the first power mechanism to F12, and sets F12= F1 x (1- (theta 0-theta 1) 2 /θ0）。
8. The use device of perfusion solution for preserving and perfusing isolated eyeball according to claim 7, characterized in that the water inlet of the circulating tube of the thermostatic unit is provided with a first temperature detector, the water inlet of the circulating tube is provided with a second temperature detector, the central control unit adjusts the heating temperature of the heating mechanism according to the temperature difference Δ t between the second temperature detector and the first temperature detector compared with the preset temperature difference, wherein,
when Δ t is less than or equal to t0, the central control unit increases the heating temperature of the heating mechanism to Tk1, and sets Tk1= Tk x (1 + (t 0- Δ t)/t 0);
when Δ t is not more than t0, the central control unit reduces the heating temperature of the heating mechanism to Tk2 setting Tk2= Tk × (1- (. DELTA.t-t 0)/t 0);
wherein k =1,2.
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