CN114113589A - Chlamydia trachomatis and mycoplasma urealyticum antigen detection kit based on magnetic particle luminescence method - Google Patents
Chlamydia trachomatis and mycoplasma urealyticum antigen detection kit based on magnetic particle luminescence method Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 239000000427 antigen Substances 0.000 title claims abstract description 25
- 102000036639 antigens Human genes 0.000 title claims abstract description 25
- 108091007433 antigens Proteins 0.000 title claims abstract description 25
- 241000606153 Chlamydia trachomatis Species 0.000 title claims abstract description 14
- 229940038705 chlamydia trachomatis Drugs 0.000 title claims abstract description 14
- 238000002796 luminescence method Methods 0.000 title claims abstract description 12
- 239000006249 magnetic particle Substances 0.000 title claims abstract description 12
- 241000204031 Mycoplasma Species 0.000 title claims abstract description 8
- 239000000243 solution Substances 0.000 claims abstract description 33
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 23
- 239000007853 buffer solution Substances 0.000 claims abstract description 19
- 239000004005 microsphere Substances 0.000 claims abstract description 19
- 239000011534 wash buffer Substances 0.000 claims abstract description 11
- 238000002474 experimental method Methods 0.000 claims abstract description 9
- 239000012224 working solution Substances 0.000 claims abstract description 9
- 230000003213 activating effect Effects 0.000 claims abstract description 6
- 239000011248 coating agent Substances 0.000 claims abstract description 6
- 238000000576 coating method Methods 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 4
- 239000011325 microbead Substances 0.000 claims description 20
- 230000004913 activation Effects 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 8
- 241000204003 Mycoplasmatales Species 0.000 claims description 6
- 239000004793 Polystyrene Substances 0.000 claims description 6
- 229920002223 polystyrene Polymers 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 5
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Substances CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 claims description 3
- CCMKPCBRNXKTKV-UHFFFAOYSA-N 1-hydroxy-5-sulfanylidenepyrrolidin-2-one Chemical compound ON1C(=O)CCC1=S CCMKPCBRNXKTKV-UHFFFAOYSA-N 0.000 claims description 3
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 claims description 3
- 108010004729 Phycoerythrin Proteins 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000002331 protein detection Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000011550 stock solution Substances 0.000 description 24
- 102000004169 proteins and genes Human genes 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 12
- 230000000087 stabilizing effect Effects 0.000 description 12
- 229920001213 Polysorbate 20 Polymers 0.000 description 10
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 10
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 10
- 239000012535 impurity Substances 0.000 description 8
- 239000008363 phosphate buffer Substances 0.000 description 8
- 238000007865 diluting Methods 0.000 description 7
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000005406 washing Methods 0.000 description 3
- 239000007836 KH2PO4 Substances 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 description 2
- 235000019800 disodium phosphate Nutrition 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000011146 sterile filtration Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56933—Mycoplasma
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56927—Chlamydia
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- Engineering & Computer Science (AREA)
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- Urology & Nephrology (AREA)
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Abstract
The invention discloses a magnetic particle luminescence method-based chlamydia trachomatis and mycoplasma urealytium antigen detection kit, and belongs to the technical field of detection reagents. The method comprises the steps of capturing microsphere mixed liquor, detecting antibodies, SA-PE, experiment buffer solution, washing buffer solution and working solution, wherein the production process comprises the steps of activating and coating prepared microspheres, preparing the detecting antibodies, preparing the SA-PE, preparing the experiment buffer solution, preparing the washing buffer solution and the working solution, so that the problem that when CT and UU antigens in a sample are detected, two detection reagents are required to be used for separately detecting the CT and UU antigens is solved, the detection time is shortened, and the detection efficiency is improved.
Description
Technical Field
The invention relates to a magnetic particle luminescence method-based chlamydia trachomatis and mycoplasma urealytium antigen detection kit, belongs to the technical field of detection reagents, and discloses a detection kit capable of simultaneously detecting two antigens, namely CT and UU, in a sample in a micropore.
Background
The CT and UU antigens are common antigens in the chlamydia trachomatis and the mycoplasma urealytium, however, with the progress of the medical level, the CT and UU antigens can be detected by the existing detection reagent, and then further treatment is carried out, however, when the CT and UU antigens in one sample are detected, two detection reagents are needed to be used for separately detecting the CT and UU antigens, so that the detection time is prolonged, and the detection efficiency is reduced.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the kit for detecting the antigens of the chlamydia trachomatis and the mycoplasma urealytium based on the magnetic particle luminescence method solves the problem that when the antigens of the CT and the UU in one sample are detected, two detection reagents are needed to be used for separately detecting the antigens of the CT and the UU.
The technical problem to be solved by the invention is realized by adopting the following technical scheme:
a detection kit for Chlamydia trachomatis and Mycoplasma urealyticum antigens based on a magnetic particle luminescence method comprises a capture microsphere mixed solution, a detection antibody, SA-PE, an experiment buffer solution, a washing buffer solution and a working solution,
(1) capturing microsphere mixed liquor: the suspension contains identifiable polystyrene microbeads, and the polystyrene microbeads are respectively coated with the following antibodies: anti-human CT antibody, anti-human UU antibody;
(2) detecting an antibody: diluting the stock solution with protein stabilizing solution 62.5 times under the condition that the concentration of the stock solution is 0.5mg/mL to ensure that the concentration is 0.8 mu g/mL;
(3) and SA-PE: diluting the stock solution with protein stabilizing solution 50 times under the condition that the concentration of the stock solution is 1mg/mL to ensure that the concentration is 2 mug/mL;
(4) working fluid: diluting the stock solution with protein stabilizing solution 6.7 times under the condition that the concentration of the stock solution is 40.6mg/mL to ensure that the concentration is 0.6 mg/mL;
(5) experiment buffer solution: adding 50g BSA, 1mL Tween-20, 3mL Proclin300 and 0.5mL RPE2520 into 1000mL of phosphate buffer, stirring for more than 30min, and performing sterile filtration with the diameter of at least 0.45 μm after the solution is clear and free of impurities;
(6) washing buffer solution: 5mL Tween-20, 3mL Proclin300 and 0.3mL RPE2520 were added to 1000mL of phosphate buffer, stirred for more than 30min, and sterile filtered to at least 0.45 μm after the solution was clear and free of impurities.
As a preferred example, the production process of the kit mainly comprises the following steps:
preparing an activation buffer solution, wherein the main components of the activation buffer solution comprise: namely 8.0g/L NaCl reagent, 2.9g/L Na2HPO4 & 12H2O reagent, 2.4g/L KCl reagent, 2.4g/L KH2PO4 reagent, 0.5g/L Tris reagent, 0.5mL/L Tween20 reagent and 0.5g/L SodiumAzide reagent.
② activation and coating of microspheres, adding 1mL of microspheres with specific numbers into a 1.5mL centrifuge tube, adding 5-50uLEDC (1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride) and 5-50uLSuLfo-NHS (N-hydroxy thiosuccinimide), adding 10-100ug of specific antibody, vortex mixing for 30 seconds, ultrasonic mixing for 30 seconds, and shaking for overnight at 2-8 ℃.
Preparing a detection antibody: when the concentration of the stock solution was 0.5mg/mL, the stock solution was diluted 62.5-fold with the protein stabilizing solution to a concentration of 0.8. mu.g/mL.
Preparing SA-PE: when the concentration of the stock solution was 1mg/mL, the stock solution was diluted 50-fold with the protein stabilizing solution to a concentration of 2. mu.g/mL.
Preparing working solution: when the concentration of the stock solution was 40.6mg/mL, the stock solution was diluted 6.7-fold with the protein stabilizing solution to a concentration of 0.6 mg/mL.
Preparing an experimental buffer solution: 50g BSA, 1mL Tween-20, 3mL Proclin300 and 0.5mL RPE2520 were added to 1000mL of phosphate buffer, stirred for more than 30min, and sterile filtered to at least 0.45 μm after the solution was clear and free of impurities.
Preparing a washing buffer solution: 5mL Tween-20, 3mL Proclin300 and 0.3mL RPE2520 were added to 1000mL of phosphate buffer, stirred for more than 30min, and sterile filtered to at least 0.45 μm after the solution was clear and free of impurities.
The invention has the beneficial effects that: the method comprises the steps of capturing microsphere mixed liquor, detecting antibodies, SA-PE, experiment buffer solution, washing buffer solution and working solution, wherein the production process comprises the steps of preparing activation buffer solution, activating and coating microspheres, preparing detection antibodies, preparing SA-PE, preparing working solution, preparing experiment buffer solution and preparing washing buffer solution, the problem that when CT and UU antigens in a sample are detected, two detection reagents are required to be used for separately detecting the CT and UU antigens is solved, the detection time is shortened, and the detection efficiency is improved.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purpose and the efficacy of the invention easily understood, the invention is further described with reference to the following embodiments.
A detection kit for Chlamydia trachomatis and Mycoplasma urealyticum antigens based on a magnetic particle luminescence method comprises a capture microsphere mixed solution, a detection antibody, SA-PE, an experiment buffer solution, a washing buffer solution and a working solution,
(1) capturing microsphere mixed liquor: the suspension contains identifiable polystyrene microbeads, and the polystyrene microbeads are respectively coated with the following antibodies: anti-human CT antibody, anti-human UU antibody;
(2) detecting an antibody: diluting the stock solution with protein stabilizing solution 62.5 times under the condition that the concentration of the stock solution is 0.5mg/mL to ensure that the concentration is 0.8 mu g/mL;
(3) and SA-PE: diluting the stock solution with protein stabilizing solution 50 times under the condition that the concentration of the stock solution is 1mg/mL to ensure that the concentration is 2 mug/mL;
(4) working fluid: diluting the stock solution with protein stabilizing solution 6.7 times under the condition that the concentration of the stock solution is 40.6mg/mL to ensure that the concentration is 0.6 mg/mL;
(5) experiment buffer solution: adding 50g BSA, 1mL Tween-20, 3mL Proclin300 and 0.5mL RPE2520 into 1000mL of phosphate buffer, stirring for more than 30min, and performing sterile filtration with the diameter of at least 0.45 μm after the solution is clear and free of impurities;
(6) washing buffer solution: 5mL Tween-20, 3mL Proclin300 and 0.3mL RPE2520 were added to 1000mL of phosphate buffer, stirred for more than 30min, and sterile filtered to at least 0.45 μm after the solution was clear and free of impurities.
The production process of the kit mainly comprises the following steps:
preparing an activation buffer solution, wherein the main components of the activation buffer solution comprise: namely 8.0g/L NaCl reagent, 2.9g/L Na2HPO4 & 12H2O reagent, 2.4g/L KCl reagent, 2.4g/L KH2PO4 reagent, 0.5g/L Tris reagent, 0.5mL/L Tween20 reagent and 0.5g/L SodiumAzide reagent;
② activation and coating of microspheres, adding 1mL of microspheres with specific numbers into a 1.5mL centrifuge tube, adding 5-50uLEDC (1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride) and 5-50uLSuLfo-NHS (N-hydroxy thiosuccinimide), adding 10-100ug of specific antibody, vortex mixing for 30 seconds, ultrasonic mixing for 30 seconds, and shaking for overnight at 2-8 ℃.
When the detection kit is produced, a table concentrator for placing 4 antibody bottles simultaneously needs to be arranged to coat microspheres with different numbers respectively, and the steps are as follows:
1) washing: performing suction filtration washing, and transferring the washed microbeads to an activation buffer solution;
2) quality control: verifying quality by using a quality control panel;
3) mixing multiple coated microbeads, washing residual microbeads on the wall of the bottle with a small amount of activating buffer solution, mixing, diluting to constant volume with the activating buffer solution, and packaging after inspection is qualified.
Preparing a detection antibody: when the concentration of the stock solution was 0.5mg/mL, the stock solution was diluted 62.5-fold with the protein stabilizing solution to a concentration of 0.8. mu.g/mL.
Preparing SA-PE: when the concentration of the stock solution was 1mg/mL, the stock solution was diluted 50-fold with the protein stabilizing solution to a concentration of 2. mu.g/mL.
Preparing working solution: when the concentration of the stock solution was 40.6mg/mL, the stock solution was diluted 6.7-fold with the protein stabilizing solution to a concentration of 0.6 mg/mL.
Preparing an experimental buffer solution: 50g BSA, 1mL Tween-20, 3mL Proclin300 and 0.5mL RPE2520 were added to 1000mL of phosphate buffer, stirred for more than 30min, and sterile filtered to at least 0.45 μm after the solution was clear and free of impurities.
Preparing a washing buffer solution: 5mL Tween-20, 3mL Proclin300 and 0.3mL RPE2520 were added to 1000mL of phosphate buffer, stirred for more than 30min, and sterile filtered to at least 0.45 μm after the solution was clear and free of impurities.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A kit for detecting chlamydia trachomatis and mycoplasma urealytium antigens based on a magnetic particle luminescence method is characterized by comprising: (1) a plurality of sealed reagent bottles which are respectively filled with capture microsphere mixed liquor, detection antibody, SA-PE, experiment buffer solution, washing buffer solution and working solution, and (2) a kit which separates and integrally packages the reagent bottles; the microbeads are polystyrene microspheres with uniform size, and the polystyrene microspheres are dyed and numbered by different fluorescent dyes, so that microbeads with different codes are obtained; the capture microsphere mixed solution contains protein detection microbeads, and the method specifically comprises the following steps:
the detection micro-bead is characterized in that anti-human CT antibodies and anti-human UU antibodies are respectively coated on the surfaces of micro-beads with different codes.
2. The kit for detecting the antigens of the chlamydia trachomatis and the mycoplasma urealytium based on the magnetic particle luminescence method according to claim 1, wherein the diameter of the microbead is 5-7 microns.
3. The kit for detecting the antigens of the chlamydia trachomatis and the mycoplasma urealytium based on the magnetic particle luminescence method as claimed in claim 1, wherein the fluorescent marker is phycoerythrin-labeled streptavidin.
4. The kit for detecting the antigens of the chlamydia trachomatis and the mycoplasma urealytium based on the magnetic particle luminescence method as claimed in claim 1, wherein the preparation method of the capture microsphere mixed solution comprises the following steps: and respectively coating different chlamydia trachomatis and mycoplasma urealytium antigens by using different coded microbeads, and mixing to prepare the detection microbeads to obtain the capture microsphere mixed solution.
5. The kit for detecting the antigens of the chlamydia trachomatis and the mycoplasma urealytium based on the magnetic particle luminescence method as claimed in claim 1, wherein the detection beads are prepared by the following method: preparing a microbead activating solution, adding any one antibody according to the proportion that each milliliter of microbead contains 10-100ug of antibody, fully mixing, coating different antibodies, and using microbeads with different codes; the microbead activating solution is prepared by adding microbeads of 1000 unit volumes into a reaction tube, and adding 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride of 5-50 unit volumes and N-hydroxy thiosuccinimide of 5-50 unit volumes for activation.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101246164A (en) * | 2008-01-29 | 2008-08-20 | 广州益善生物技术有限公司 | Alzheimer''s disease early diagnosis liquid phase chip and method for producing the same |
| CN103472229A (en) * | 2013-09-17 | 2013-12-25 | 武汉生之源生物科技有限公司 | Magnetic particle chemiluminescence immune assay detection kit for carcino-embryonic antigen and detection method of detection kit |
| CN204142733U (en) * | 2014-07-31 | 2015-02-04 | 李克生 | Chlamydia trachomatis, Ureaplasma urealyticum and mycoplasma hominis combined detection kit |
| CN105203769A (en) * | 2014-08-18 | 2015-12-30 | 董俊 | Method and kit for fast detection of human chlamydia pneumoniae antigen based on magnetic resolution and quantum dot labelling |
| CN105911291A (en) * | 2016-04-26 | 2016-08-31 | 上海中信亚特斯诊断试剂有限公司 | Urine trace protein detection kit and detection method thereof |
| CN106290884A (en) * | 2015-06-15 | 2017-01-04 | 江苏戴格诺思生物技术有限公司 | A kind of Ureaplasma urealyticum gold mark detection kit and detection method |
| CN107238711A (en) * | 2017-05-18 | 2017-10-10 | 无锡市精神卫生中心 | A kind of diagnostic kit and its detection method for detecting Alzheimer disease peripheral blood protein marker |
| CN109116031A (en) * | 2018-07-25 | 2019-01-01 | 滴准生物科技（常州）有限公司 | A kind of detection kit can detect a variety of prenatal and postnatal care pathogen IgG antibodies |
| CN109142743A (en) * | 2018-07-25 | 2019-01-04 | 滴准生物科技（常州）有限公司 | A kind of multinomial detection kit for exempting from liver antibody certainly |
| CN111157725A (en) * | 2020-01-09 | 2020-05-15 | 南京拂晓生物科技有限公司 | Human Legumain chemiluminescence detection kit and application thereof |
-
2021
- 2021-10-25 CN CN202111240279.7A patent/CN114113589A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101246164A (en) * | 2008-01-29 | 2008-08-20 | 广州益善生物技术有限公司 | Alzheimer''s disease early diagnosis liquid phase chip and method for producing the same |
| CN103472229A (en) * | 2013-09-17 | 2013-12-25 | 武汉生之源生物科技有限公司 | Magnetic particle chemiluminescence immune assay detection kit for carcino-embryonic antigen and detection method of detection kit |
| CN204142733U (en) * | 2014-07-31 | 2015-02-04 | 李克生 | Chlamydia trachomatis, Ureaplasma urealyticum and mycoplasma hominis combined detection kit |
| CN105203769A (en) * | 2014-08-18 | 2015-12-30 | 董俊 | Method and kit for fast detection of human chlamydia pneumoniae antigen based on magnetic resolution and quantum dot labelling |
| CN106290884A (en) * | 2015-06-15 | 2017-01-04 | 江苏戴格诺思生物技术有限公司 | A kind of Ureaplasma urealyticum gold mark detection kit and detection method |
| CN105911291A (en) * | 2016-04-26 | 2016-08-31 | 上海中信亚特斯诊断试剂有限公司 | Urine trace protein detection kit and detection method thereof |
| CN107238711A (en) * | 2017-05-18 | 2017-10-10 | 无锡市精神卫生中心 | A kind of diagnostic kit and its detection method for detecting Alzheimer disease peripheral blood protein marker |
| CN109116031A (en) * | 2018-07-25 | 2019-01-01 | 滴准生物科技（常州）有限公司 | A kind of detection kit can detect a variety of prenatal and postnatal care pathogen IgG antibodies |
| CN109142743A (en) * | 2018-07-25 | 2019-01-04 | 滴准生物科技（常州）有限公司 | A kind of multinomial detection kit for exempting from liver antibody certainly |
| CN111157725A (en) * | 2020-01-09 | 2020-05-15 | 南京拂晓生物科技有限公司 | Human Legumain chemiluminescence detection kit and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 杨爱景;: "解脲支原体 沙眼衣原体感染与男性不育关系的探讨", 中国艾滋病性病, no. 01, 26 February 2006 (2006-02-26), pages 47 - 48 * |
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