CN114113084A - Real-time accompanying film reading method based on digital pathological image and storage device - Google Patents
Real-time accompanying film reading method based on digital pathological image and storage device Download PDFInfo
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Abstract
The invention relates to the technical field of digital pathology image processing, in particular to a real-time accompanying film reading method and storage equipment based on digital pathology images. The real-time accompanying film reading method based on the digital pathological image comprises the following steps: acquiring a digital pathological image to be read; and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well. By the method, a plurality of slices can be observed at one time in the pathological diagnosis process, and the same adjustment can be carried out among the slices, so that the comparison among the slices becomes simpler and more convenient, and the pathological diagnosis time according to the slices is greatly shortened.
Description
Technical Field
The invention relates to the technical field of digital pathology image processing, in particular to a real-time accompanying film reading method and storage equipment based on digital pathology images.
Background
Pathological diagnosis is to take tumor specimen cut out by operation or autopsy, fix and stain the specimen, and then carry out histological examination under microscope to diagnose diseases, and more particularly, to take tumor tissue by clamping, cutting or cutting method before treatment on living human body, fix and stain the specimen, and then carry out pathological diagnosis under microscope. Despite the rapid development of various imaging techniques, pathological diagnosis, which is known as "gold standard", is the final diagnosis of diseases, is still the most reliable of various examination methods for tumors.
Currently, the main tools for pathological diagnosis are H & E and immunohistochemistry. A sample is usually subjected to immunohistochemical staining using a series of immunohistochemical reagents (antibody reagents), and the pathologist microscopically reads sections of the various immunohistochemical stains to obtain a final diagnosis. In the process, a pathologist firstly reads the H & E section to determine the information of the form, the histological distribution and the like of the pathological tissues, then judges the corresponding pathological tissues on the multiple immunohistochemical staining sections to obtain the information of the biomarker expression condition of the pathological tissues, gives the pathological diagnosis result of a patient sample by integrating the tissue morphology and the expression condition of the biomarker, and provides a basis for clinical treatment.
However, during microscopic observation, only one slice can be observed at a time, and therefore, it is time-consuming and labor-consuming to achieve accurate and objective comparison between an H & E stained slice and an immunohistochemically stained slice, or between multiple immunohistochemically stained slices.
Disclosure of Invention
Therefore, a real-time accompanying film reading method based on digital pathological images is needed to be provided, so as to solve the technical problem that the conventional pathological section film reading method only can be used for observing under a microscope and only can be used for observing one film at a time, so that the observation of multiple films or the comparison of multiple films is time-consuming and labor-consuming, and the specific technical scheme is as follows:
a real-time accompanying film reading method based on digital pathological images comprises the following steps:
acquiring a digital pathological image to be read;
and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well.
Further, the "matching digital pathological images corresponding to two or more slices in the same tissue so that when one of the digital pathological images is adjusted, the other digital pathological images are adjusted as well", specifically includes the steps of:
adjusting the angles of digital pathological images corresponding to different slices in the same tissue until the overlapping degrees of different digital pathological images reach a preset condition;
judging whether the digital pathological image of any one section is adjusted, if so, performing the same adjustment on the digital pathological images of other sections, wherein the adjustment includes but is not limited to: zooming out, zooming in, and changing positions.
Further, the digital pathological image to be read is generated as follows:
preparing a tissue slice;
and carrying out different staining treatments on the prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read.
Further, the "tissue slice preparation" specifically includes the steps of:
the method comprises the steps of trimming wax blocks of preset organ tissues, slicing the wax blocks, determining the thickness to be 3 mu m, floating the slices in cold water, naturally unfolding the slices, transferring the separated slices into warm water at 45 ℃ for spreading for 30 seconds, mounting the slices by using a glass slide treated by polylysine, baking the prepared tissue chips in an oven at 65 ℃ for 2 hours, taking out the tissue chips, cooling the tissue chips at room temperature, and storing the tissue chips in a refrigerator at-4 ℃.
Further, the method for performing different staining treatments on the prepared tissue section and scanning the stained section to generate a digital pathological image to be read includes the following steps:
taking a slice, dewaxing by using conventional xylene for 3 times, wherein each time is 6 minutes, hydrating in 100%, 95% and 85% gradient ethanol for 3 minutes, finally washing by using tap water, spin-drying the slice, dyeing in hematoxylin staining solution for 4 minutes, taking out, returning blue by using PSB for 25 seconds, throwing away PBS, dyeing the slice in eosin staining solution for 1 minute, washing by using PBS for 3 multiplied by 3 minutes, throwing away the PBS, sequentially placing the slice in 95% alcohol for 5 minutes, 95% alcohol II for 5 minutes, absolute ethanol for 5 minutes, xylene for 5 minutes, dehydrating and transparentizing in xylene for 5 minutes, taking out the slice from the xylene, slightly drying, sealing the slice by using neutral gum, and scanning the slice by using a tissue slice scanner to form a digital pathological image to be read;
or
Taking a slice, dewaxing 3 times by conventional xylene, 6 minutes each time, hydrating in 100%, 95%, 85% gradient ethanol, 3 minutes each time, finally washing by tap water, performing antigen retrieval, then putting the slice into a wet box, washing by PBS for 3 x 3 minutes, dropwise adding 3% H2O2, incubating for 10 minutes, washing by PBS for 3 x 3 minutes, spin-drying the slice, dropwise adding an immunohistochemical antibody reagent diluted in a proper proportion, incubating for 1 hour at room temperature (25 ℃), washing by PBS for 3 x 3 minutes, dropwise adding a secondary antibody, incubating for 15-30 minutes at room temperature, washing by PBS for 3 x 3 minutes, throwing off the PBS, and developing by using a freshly prepared AP-Red developing solution for 5-15 minutes. Hematoxylin counterstain for 25 seconds, PBS rewet for 30 seconds, and aqueous mounting tablet mounting. And scanning the tissue section by using a tissue section scanner to form a digital pathological image to be read.
In order to solve the technical problem, the storage device is further provided, and the specific technical scheme is as follows:
a storage device having stored therein a set of instructions for performing:
acquiring a digital pathological image to be read;
and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well.
Further, the set of instructions is further for performing:
the method for matching the digital pathological images corresponding to more than two slices in the same tissue to enable other digital pathological images to be adjusted the same as the digital pathological image is adjusted when one digital pathological image is adjusted specifically comprises the following steps:
adjusting the angles of digital pathological images corresponding to different slices in the same tissue until the overlapping degrees of different digital pathological images reach a preset condition;
judging whether the digital pathological image of any one section is adjusted, if so, performing the same adjustment on the digital pathological images of other sections, wherein the adjustment includes but is not limited to: zooming out, zooming in, and changing positions.
Further, the digital pathological image to be read is generated as follows:
preparing a tissue slice;
and carrying out different staining treatments on the prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read.
Further, the set of instructions is further for performing:
the tissue slice preparation method specifically comprises the following steps:
the method comprises the steps of trimming wax blocks of preset organ tissues, slicing the wax blocks, determining the thickness to be 3 mu m, floating the slices in cold water, naturally unfolding the slices, transferring the separated slices into warm water at 45 ℃ for spreading for 30 seconds, mounting the slices by using a glass slide treated by polylysine, baking the prepared tissue chips in an oven at 65 ℃ for 2 hours, taking out the tissue chips, cooling the tissue chips at room temperature, and storing the tissue chips in a refrigerator at-4 ℃.
Further, the set of instructions is further for performing:
the method comprises the following steps of performing different staining treatments on prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read, and specifically comprises the following steps:
taking a slice, dewaxing by using conventional xylene for 3 times, wherein each time is 6 minutes, hydrating in 100%, 95% and 85% gradient ethanol for 3 minutes, finally washing by using tap water, spin-drying the slice, dyeing in hematoxylin staining solution for 4 minutes, taking out, returning blue by using PSB for 25 seconds, throwing away PBS, dyeing the slice in eosin staining solution for 1 minute, washing by using PBS for 3 multiplied by 3 minutes, throwing away the PBS, sequentially placing the slice in 95% alcohol for 5 minutes, 95% alcohol II for 5 minutes, absolute ethanol for 5 minutes, xylene for 5 minutes, dehydrating and transparentizing in xylene for 5 minutes, taking out the slice from the xylene, slightly drying, sealing the slice by using neutral gum, and scanning the slice by using a tissue slice scanner to form a digital pathological image to be read;
or
Taking a slice, dewaxing 3 times by conventional xylene, 6 minutes each time, hydrating in 100%, 95%, 85% gradient ethanol, 3 minutes each time, finally washing by tap water, performing antigen retrieval, then putting the slice into a wet box, washing by PBS for 3 x 3 minutes, dropwise adding 3% H2O2, incubating for 10 minutes, washing by PBS for 3 x 3 minutes, spin-drying the slice, dropwise adding an immunohistochemical antibody reagent diluted in a proper proportion, incubating for 1 hour at room temperature (25 ℃), washing by PBS for 3 x 3 minutes, dropwise adding a secondary antibody, incubating for 15-30 minutes at room temperature, washing by PBS for 3 x 3 minutes, throwing off the PBS, and developing by using a freshly prepared AP-Red developing solution for 5-15 minutes. Hematoxylin counterstain for 25 seconds, PBS rewet for 30 seconds, and aqueous mounting tablet mounting. And scanning the tissue section by using a tissue section scanner to form a digital pathological image to be read.
The invention has the beneficial effects that: a real-time accompanying film reading method based on digital pathological images comprises the following steps: acquiring a digital pathological image to be read; and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well. By the method, a plurality of slices can be observed at one time in the pathological diagnosis process, and the same adjustment can be carried out among the slices, so that the comparison among the slices becomes simpler and more convenient, and the pathological diagnosis time according to the slices is greatly shortened.
Drawings
FIG. 1 is a flow chart of a method for real-time concomitant review of digital pathology-based images, according to an embodiment;
FIG. 2a is a schematic diagram of two different slices before matching according to an embodiment;
FIG. 2b is a diagram illustrating two different slices after matching according to an embodiment;
FIG. 3a is a schematic diagram of three different slices before matching according to an embodiment;
FIG. 3b is a diagram illustrating matching of three different slices according to an embodiment;
FIG. 4 is an enlarged schematic view of the same location of two slices according to an embodiment;
FIG. 5 is an enlarged schematic view of the same location of three slices according to an embodiment;
fig. 6 is a block diagram of a storage device according to an embodiment.
Description of reference numerals:
600. a storage device.
Detailed Description
To explain technical contents, structural features, and objects and effects of the technical solutions in detail, the following detailed description is given with reference to the accompanying drawings in conjunction with the embodiments.
Referring to fig. 1 to 5, in the present embodiment, a real-time accompanying radiographing method based on digital pathological image can be applied to a storage device, including but not limited to: personal computers, servers, general purpose computers, special purpose computers, network devices, embedded devices, programmable devices, intelligent mobile terminals, etc. The specific technical scheme is as follows:
step S101: and acquiring a digital pathological image to be read.
Wherein the digital pathology image to be read is generated as follows:
preparing a tissue slice;
and carrying out different staining treatments on the prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read.
In the present embodiment, the tissue section is exemplified by a gastric cancer tissue. The following is developed:
slicing gastric cancer tissue wax block, setting the thickness to be 3 μm, floating the slices in cold water, naturally unfolding, transferring the separated slices into 45 ℃ warm water for 30 seconds, pasting the slices with polylysine-treated glass slides, baking the prepared tissue chips in a 65 ℃ oven for 2 hours, taking out the tissue chips, cooling at room temperature, and storing in a-4 ℃ refrigerator.
In this embodiment, the sections are stained mainly for two types: h & E staining and immunohistochemical staining.
H & E staining and scanning
One section was taken and dewaxed 3 times in normal xylene, 6 minutes each time, hydrated in 100%, 95%, 85% gradient ethanol for 3 minutes each time, and finally rinsed with tap water.
Spin-drying the slices, placing the slices in hematoxylin staining solution for staining for 4 minutes, taking out the slices, returning blue with PSB for 25 seconds, throwing away PBS, placing the slices in eosin staining solution for staining for 1 minute, washing the slices with PBS for 3X 3 minutes, throwing away PBS, placing the slices in 95% alcohol for 5 minutes, 95% alcohol II for 5 minutes, absolute ethyl alcohol for 5 minutes, xylene for 5 minutes, dehydrating and transparentizing the slices in xylene for 5 minutes, taking out the slices from the xylene, slightly drying the slices, and sealing the slices with neutral gum. And scanning the tissue section by using a tissue section scanner to form a digital pathological image to be read.
Immunohistochemical staining and scanning
One section was taken and dewaxed 3 times in normal xylene, 6 minutes each time, hydrated in 100%, 95%, 85% gradient ethanol for 3 minutes each time, and finally rinsed with tap water. Antigen retrieval was performed and the sections were then placed in a wet box and washed 3 x 3 min with PBS. Incubate dropwise with 3% H2O2 for 10 min and wash with PBS for 3X 3 min.
Spin-drying the slices, dripping an immunohistochemical antibody reagent diluted in a proper proportion, incubating for 1 hour at room temperature (25 ℃), washing for 3 x 3 minutes by PBS, dripping secondary antibody, incubating for 15-30 minutes at room temperature, washing for 3 x 3 minutes by PBS, throwing off the PBS, and developing for 5-15 minutes by using a freshly prepared AP-Red developing solution. Hematoxylin counterstain for 25 seconds, PBS bluing for 30 seconds. And (5) sealing the water-based sealing agent. And scanning the tissue section by using a tissue section scanner to form a digital pathological image to be read.
After the digital pathological image to be read is acquired, step S102 is executed: and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well. The method specifically comprises the following steps:
the angle adjustment is performed on the digital pathological images corresponding to different sections in the same tissue until the overlapping degree of the different digital pathological images reaches a preset condition, in the embodiment, the overlapping degree of two digital pathological images reaches 95%, in other embodiments, the adjustment can be performed according to actual conditions, and the basic principle is that the tissues in two or more sections can be basically overlapped. Fig. 2a is a schematic diagram before matching two different slices, and fig. 2b is a schematic diagram after matching two different slices. Fig. 3a is a schematic diagram showing three different slices before matching, and fig. 3b is a schematic diagram showing three different slices after matching.
Judging whether the digital pathological image of any one section is adjusted, if so, performing the same adjustment on the digital pathological images of other sections, wherein the adjustment includes but is not limited to: zooming out, zooming in, and changing positions. As shown in FIG. 4, the two slices are enlarged at the same position, and as shown in FIG. 5, the three slices are enlarged at the same position.
A real-time accompanying film reading method based on digital pathological images comprises the following steps: acquiring a digital pathological image to be read; and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well. By the method, a plurality of slices can be observed at one time in the pathological diagnosis process, and the same adjustment can be carried out among the slices, so that the comparison among the slices becomes simpler and more convenient, and the pathological diagnosis time according to the slices is greatly shortened.
Referring to fig. 2a to 6, an embodiment of a memory device 600 is as follows:
a storage device 600 having stored therein a set of instructions for performing:
and acquiring a digital pathological image to be read.
Wherein the digital pathology image to be read is generated as follows:
preparing a tissue slice;
and carrying out different staining treatments on the prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read.
In the present embodiment, the tissue section is exemplified by a gastric cancer tissue. The following is developed:
slicing gastric cancer tissue wax block, setting the thickness to be 3 μm, floating the slices in cold water, naturally unfolding, transferring the separated slices into 45 ℃ warm water for 30 seconds, pasting the slices with polylysine-treated glass slides, baking the prepared tissue chips in a 65 ℃ oven for 2 hours, taking out the tissue chips, cooling at room temperature, and storing in a-4 ℃ refrigerator.
In this embodiment, the sections are stained mainly for two types: h & E staining and immunohistochemical staining.
H & E staining and scanning
One section was taken and dewaxed 3 times in normal xylene, 6 minutes each time, hydrated in 100%, 95%, 85% gradient ethanol for 3 minutes each time, and finally rinsed with tap water.
Spin-drying the slices, placing the slices in hematoxylin staining solution for staining for 4 minutes, taking out the slices, returning blue with PSB for 25 seconds, throwing away PBS, placing the slices in eosin staining solution for staining for 1 minute, washing the slices with PBS for 3X 3 minutes, throwing away PBS, placing the slices in 95% alcohol for 5 minutes, 95% alcohol II for 5 minutes, absolute ethyl alcohol for 5 minutes, xylene for 5 minutes, dehydrating and transparentizing the slices in xylene for 5 minutes, taking out the slices from the xylene, slightly drying the slices, and sealing the slices with neutral gum. And scanning the tissue section by using a tissue section scanner to form a digital pathological image to be read.
Immunohistochemical staining and scanning
One section was taken and dewaxed 3 times in normal xylene, 6 minutes each time, hydrated in 100%, 95%, 85% gradient ethanol for 3 minutes each time, and finally rinsed with tap water. Antigen retrieval was performed and the sections were then placed in a wet box and washed 3 x 3 min with PBS. Incubate dropwise with 3% H2O2 for 10 min and wash with PBS for 3X 3 min.
Spin-drying the slices, dripping an immunohistochemical antibody reagent diluted in a proper proportion, incubating for 1 hour at room temperature (25 ℃), washing for 3 x 3 minutes by PBS, dripping secondary antibody, incubating for 15-30 minutes at room temperature, washing for 3 x 3 minutes by PBS, throwing off the PBS, and developing for 5-15 minutes by using a freshly prepared AP-Red developing solution. Hematoxylin counterstain for 25 seconds, PBS bluing for 30 seconds. And (5) sealing the water-based sealing agent. And scanning the tissue section by using a tissue section scanner to form a digital pathological image to be read.
After the digital pathological images to be read are obtained, the digital pathological images corresponding to more than two slices in the same tissue are matched, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well. The method specifically comprises the following steps:
the angle adjustment is performed on the digital pathological images corresponding to different sections in the same tissue until the overlapping degree of the different digital pathological images reaches a preset condition, in the embodiment, the overlapping degree of two digital pathological images reaches 95%, in other embodiments, the adjustment can be performed according to actual conditions, and the basic principle is that the tissues in two or more sections can be basically overlapped. Fig. 2a is a schematic diagram before matching two different slices, and fig. 2b is a schematic diagram after matching two different slices. Fig. 3a is a schematic diagram showing three different slices before matching, and fig. 3b is a schematic diagram showing three different slices after matching.
Judging whether the digital pathological image of any one section is adjusted, if so, performing the same adjustment on the digital pathological images of other sections, wherein the adjustment includes but is not limited to: zooming out, zooming in, and changing positions. As shown in FIG. 4, the two slices are enlarged at the same position, and as shown in FIG. 5, the three slices are enlarged at the same position.
A storage device 600 having stored therein a set of instructions for performing: acquiring a digital pathological image to be read; and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well. Through above storage device for at pathological diagnosis in-process, can once observe many sections, and can do same adjustment between the section, will make the comparison between many sections become more simple and convenient, will reduce greatly and carry out pathological diagnosis's time according to the section.
It should be noted that, although the above embodiments have been described herein, the invention is not limited thereto. Therefore, based on the innovative concepts of the present invention, the technical solutions of the present invention can be directly or indirectly applied to other related technical fields by making changes and modifications to the embodiments described herein, or by using equivalent structures or equivalent processes performed in the content of the present specification and the attached drawings, which are included in the scope of the present invention.
Claims (10)
1. A real-time accompanying film reading method based on digital pathological images is characterized by comprising the following steps:
acquiring a digital pathological image to be read;
and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well.
2. The method for real-time concomitant interpretation based on digital pathological images according to claim 1, wherein the step of matching the digital pathological images corresponding to two or more slices in the same tissue so that when one digital pathological image is adjusted, the other digital pathological images are adjusted as well, further comprises the steps of:
adjusting the angles of digital pathological images corresponding to different slices in the same tissue until the overlapping degrees of different digital pathological images reach a preset condition;
judging whether the digital pathological image of any one section is adjusted, if so, performing the same adjustment on the digital pathological images of other sections, wherein the adjustment includes but is not limited to: zooming out, zooming in, and changing positions.
3. The method for real-time concomitant interpretation based on digital pathological image as claimed in claim 1, wherein the digital pathological image to be read is generated as follows:
preparing a tissue slice;
and carrying out different staining treatments on the prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read.
4. The real-time concomitant interpretation method based on digital pathological image as claimed in claim 3, wherein said "tissue section preparation" further comprises the following steps:
the method comprises the steps of trimming wax blocks of preset organ tissues, slicing the wax blocks, determining the thickness to be 3 mu m, floating the slices in cold water, naturally unfolding the slices, transferring the separated slices into warm water at 45 ℃ for spreading for 30 seconds, mounting the slices by using a glass slide treated by polylysine, baking the prepared tissue chips in an oven at 65 ℃ for 2 hours, taking out the tissue chips, cooling the tissue chips at room temperature, and storing the tissue chips in a refrigerator at-4 ℃.
5. The real-time accompanying radiograph reading method based on the digital pathological image as claimed in claim 4, wherein the method comprises the following steps:
taking a slice, dewaxing by using conventional xylene for 3 times, wherein each time is 6 minutes, hydrating in 100%, 95% and 85% gradient ethanol for 3 minutes, finally washing by using tap water, spin-drying the slice, dyeing in hematoxylin staining solution for 4 minutes, taking out, returning blue by using PSB for 25 seconds, throwing away PBS, dyeing the slice in eosin staining solution for 1 minute, washing by using PBS for 3 multiplied by 3 minutes, throwing away the PBS, sequentially placing the slice in 95% alcohol for 5 minutes, 95% alcohol II for 5 minutes, absolute ethanol for 5 minutes, xylene for 5 minutes, dehydrating and transparentizing in xylene for 5 minutes, taking out the slice from the xylene, slightly drying, sealing the slice by using neutral gum, and scanning the slice by using a tissue slice scanner to form a digital pathological image to be read;
or
Taking a slice, dewaxing for 3 times by conventional xylene, carrying out 6 minutes each time, hydrating in 100%, 95%, 85% gradient ethanol for 3 minutes each time, finally washing with tap water, carrying out antigen retrieval, then putting the slice into a wet box, washing with PBS for 3 x 3 minutes, dropwise adding 3% H2O2, incubating for 10 minutes, washing with PBS for 3 x 3 minutes, spin-drying the slice, dropwise adding an immunohistochemical antibody reagent diluted in a proper proportion, incubating for 1 hour at room temperature (25 ℃), washing with PBS for 3 x 3 minutes, dropwise adding a secondary antibody, incubating for 15-30 minutes at room temperature, washing with PBS for 3 x 3 minutes, throwing off the PBS, developing with a freshly prepared AP-Red developing solution for 5-15 minutes, counterstaining with hematoxylin 25 seconds, rewetting with PBS for 30 seconds, sealing a water tablet, and scanning the tissue slice by a tissue slice scanner to form a digital pathological image to be read.
6. A storage device having a set of instructions stored therein, the set of instructions being operable to perform:
acquiring a digital pathological image to be read;
and matching the digital pathological images corresponding to more than two slices in the same tissue, so that when one digital pathological image is adjusted, other digital pathological images are adjusted as well.
7. The storage device of claim 6, wherein the set of instructions is further configured to perform:
the method for matching the digital pathological images corresponding to more than two slices in the same tissue to enable other digital pathological images to be adjusted the same as the digital pathological image is adjusted when one digital pathological image is adjusted specifically comprises the following steps:
adjusting the angles of digital pathological images corresponding to different slices in the same tissue until the overlapping degrees of different digital pathological images reach a preset condition;
judging whether the digital pathological image of any one section is adjusted, if so, performing the same adjustment on the digital pathological images of other sections, wherein the adjustment includes but is not limited to: zooming out, zooming in, and changing positions.
8. The storage device of claim 6, wherein said digital pathology image to be read is generated as follows:
preparing a tissue slice;
and carrying out different staining treatments on the prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read.
9. The storage device of claim 8, wherein the set of instructions is further configured to perform:
the tissue slice preparation method specifically comprises the following steps:
the method comprises the steps of trimming wax blocks of preset organ tissues, slicing the wax blocks, determining the thickness to be 3 mu m, floating the slices in cold water, naturally unfolding the slices, transferring the separated slices into warm water at 45 ℃ for spreading for 30 seconds, mounting the slices by using a glass slide treated by polylysine, baking the prepared tissue chips in an oven at 65 ℃ for 2 hours, taking out the tissue chips, cooling the tissue chips at room temperature, and storing the tissue chips in a refrigerator at-4 ℃.
10. The storage device of claim 9, wherein the set of instructions is further configured to perform:
the method comprises the following steps of performing different staining treatments on prepared tissue sections, and scanning the stained sections to generate digital pathological images to be read, and specifically comprises the following steps:
taking a slice, dewaxing by using conventional xylene for 3 times, wherein each time is 6 minutes, hydrating in 100%, 95% and 85% gradient ethanol for 3 minutes, finally washing by using tap water, spin-drying the slice, dyeing in hematoxylin staining solution for 4 minutes, taking out, returning blue by using PSB for 25 seconds, throwing away PBS, dyeing the slice in eosin staining solution for 1 minute, washing by using PBS for 3 multiplied by 3 minutes, throwing away the PBS, sequentially placing the slice in 95% alcohol for 5 minutes, 95% alcohol II for 5 minutes, absolute ethanol for 5 minutes, xylene for 5 minutes, dehydrating and transparentizing in xylene for 5 minutes, taking out the slice from the xylene, slightly drying, sealing the slice by using neutral gum, and scanning the slice by using a tissue slice scanner to form a digital pathological image to be read;
or
Taking a slice, dewaxing for 3 times by conventional xylene, carrying out 6 minutes each time, hydrating in 100%, 95%, 85% gradient ethanol for 3 minutes each time, finally washing with tap water, carrying out antigen retrieval, then putting the slice into a wet box, washing with PBS for 3 x 3 minutes, dropwise adding 3% H2O2, incubating for 10 minutes, washing with PBS for 3 x 3 minutes, spin-drying the slice, dropwise adding an immunohistochemical antibody reagent diluted in a proper proportion, incubating for 1 hour at room temperature (25 ℃), washing with PBS for 3 x 3 minutes, dropwise adding a secondary antibody, incubating for 15-30 minutes at room temperature, washing with PBS for 3 x 3 minutes, throwing off the PBS, developing with a freshly prepared AP-Red developing solution for 5-15 minutes, counterstaining with hematoxylin 25 seconds, rewetting with PBS for 30 seconds, sealing a water tablet, and scanning the tissue slice by a tissue slice scanner to form a digital pathological image to be read.
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| PCT/CN2022/101124 WO2023082650A1 (en) | 2021-11-11 | 2022-06-24 | Real-time concomitant slice reading method based on digital pathological image, and storage device |
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