CN114107215A - Method for promoting mesenchymal stem cell cartilage differentiation by regulating and controlling inflammation through carbon dot mediated siTnf alpha - Google Patents
Method for promoting mesenchymal stem cell cartilage differentiation by regulating and controlling inflammation through carbon dot mediated siTnf alpha Download PDFInfo
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Abstract
The invention discloses a carbon dot mediated siTnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation comprises the following steps: adding linear polyethyleneimine into the fluorescent carbon dot solution and uniformly mixing; adding the centrifuged supernatant into a desalting column, and collecting eluent to obtain a fluorescent carbon dot-linear polyethyleneimine composite material solution; adding si into the fluorescent carbon dot-linearized polyethyleneimine composite material solutionTnfαReacting for 20-40min to obtain the fluorescent carbon dot-linear polyethyleneimine-siTnfαA composite material; the third generation bone marrow mesenchymal stem cells are divided into 1 × 104‑105The density of the hole is inoculated on a cell culture plate, and the cell culture plate is added withSerum, double anti-a-MEM medium; the fluorescent carbon dots-the linearized polyethyleneimine-siTnfαThe composite material is added into bone marrow mesenchymal stem cells, and a complete culture medium is replaced after transfection. The invention has good effect in repairing articular cartilage defect.
Description
Technical Field
The invention relates to the technical field of biology, in particular to carbon dot mediated siTnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation.
Background
Articular cartilage is a dense connective tissue without vascularity, nervous and lymphatic distribution, contains a small amount of chondrocytes, has a limited supply of nutrients, and has a very limited ability to regenerate once damaged by trauma or degenerative arthritis. Currently, most repair methods produce fibrocartilage only, which temporarily relieves joint pain, but has insufficient long-term function and may even increase the risk of further joint damage. In addition, autologous chondrocyte transplantation successfully promotes cartilage repair, but the cell supply is insufficient, causing new damage to the donor site during in vitro harvesting and expansion and the chondrocytes are easily dedifferentiated. Therefore, the current tissue engineering regeneration repair technology including gene therapy has gradually become one of the key regeneration repair technologies for cartilage defect treatment. The cartilage tissue regeneration engineering technology comprises a scaffold, a cell source and a growth factor, wherein the cell source is one of the most important components in the cartilage tissue engineering, and how to obtain seed cells with sufficient biological activity is a problem at present.
The bone marrow mesenchymal stem cells have self-renewal and maintain the multi-directional differentiation capability of the cells, and can be differentiated into a plurality of cell types such as chondrocytes, osteoblasts, adipocytes and muscle cells. Therefore, the bone marrow mesenchymal stem cells are used as seed cells to treat the cartilage injury, which is a promising method. But is often accompanied by inflammatory reactions during cartilage repair, thereby preventing cartilage regeneration. In this process, tumor necrosis factor α (TNF α) often plays an important role as a pro-inflammatory cytokine. Although the bone marrow mesenchymal stem cells themselves can produce immune regulatory factors to regulate the progress of inflammation, the immune regulatory capacity of the bone marrow mesenchymal stem cells is very limited. Therefore, the use of bone marrow mesenchymal stem cells as tissue engineering seed cells for the treatment of cartilage defects still presents a great challenge.
Disclosure of Invention
The invention provides a carbon dot mediated si for solving the problems in the prior artTnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation.
The invention adopts the following technical scheme.
Carbon dot mediated siTnfαMethod for regulating inflammation and promoting mesenchymal stem cell cartilage differentiationThe method comprises the following steps:
a. mixing the fluorescent carbon dots with a PBS solution, wherein the concentration is 150-250 ug/mL; adding linear polyethyleneimine into the fluorescent carbon dot solution, and stirring the mixed solution at 30-40 ℃ for 0.5-1.5 h;
b. 6000-;
c. adding si into the fluorescent carbon dot-linear polyethyleneimine composite material solution obtained in the step bTnfαMixing, reacting for 20-40min to obtain fluorescent carbon dot-linear polyethyleneimine-siTnfαA composite material;
d. the third generation bone marrow mesenchymal stem cells are divided into 1 × 104-105The density of each well was plated on a cell culture plate at 37 ℃ with 5% CO2Culturing overnight under the condition, removing the culture medium, and adding a serum-free and double-resistant alpha-MEM culture medium;
e. c, the fluorescent carbon dots-linearized polyethyleneimine-si obtained in the step cTnfαThe composite material is added to a cell culture plate containing bone marrow mesenchymal stem cells, and a complete culture medium is replaced after transfection.
Further, in the step a, the addition amount of the linear polyethyleneimine is 1/5-2/5 of the volume of the fluorescent carbon dot solution.
Further, in the step b, the fluorescent carbon dot-linearized polyethyleneimine composite material solution is filtered by using a 0.22 μm membrane.
Further, in step c, siTnfαThe final concentration of (a) is 100 ng/ml.
Further, in step c, siTnfαSelecting Si modified by sulfydrylTnfα。
Further, in step d, the method for isolating and culturing the mesenchymal stem cells comprises the following steps: repeatedly flushing the marrow cavity with stem cell culture medium containing heparin anticoagulant under aseptic condition, collecting marrow suspension, diluting with D-Hanks solution, adding Ficoll separating medium into centrifuge tube, slowly adding diluted marrow suspension onto Ficoll separating medium, centrifuging at 1500-2000 rpm/min for 10-20 min, collecting enriched fractionBoundary layer cells of the nucleated cells were washed with D-Hanks solution to adjust the cell concentration to 1X 104-105Perml inoculated in a culture flask at 37 ℃ with 5% CO2And carrying out standing culture under the saturated humidity condition.
Further, in step d, the passage method of the mesenchymal stem cells of the bone marrow is as follows: the cells grow to 80-90% of the bottom of the bottle for passage, the cells are washed by sterile PBS and then added with 0.25% trypsin solution, digestion is stopped after 1-2min, suspension cells are blown, suspension is collected, centrifugation is carried out at 1200-1500rpm/min for 5-8min, and supernatant is discarded; cells were suspended in culture flasks using complete medium and passaged once every 2-3 days.
The present invention obtains the following advantageous effects.
The invention uses the fluorescent carbon dot nano material as a carrier, and transfects the mesenchymal stem cells with TNF alpha siRNA to inhibit inflammatory reaction in the cartilage repair process and promote the differentiation of the mesenchymal stem cells into cartilage cells so as to promote the regeneration of cartilage and repair cartilage defect, thereby having wide application prospect clinically.
Detailed Description
Si of the inventionTnfαPurchased from Biotechnology engineering (Shanghai) Ltd.
The present invention will be further described with reference to examples.
Example 1
Carbon dot mediated siTnfαA method for regulating and controlling inflammation to promote mesenchymal stem cell cartilage differentiation comprises the following steps:
a. mixing the fluorescent carbon dots with a PBS solution, wherein the concentration is 150 ug/mL; adding linear polyethyleneimine into the fluorescent carbon dot solution, wherein the addition amount of the linear polyethyleneimine is 1/5 of the volume of the fluorescent carbon dot solution, and stirring the mixed solution at 40 ℃ for 0.5 h;
b. centrifuging at 6000rpm/min for 5min, adding the centrifuged supernatant into a desalting column, eluting by adopting MES solution and filtering by adopting a 0.22 mu m membrane to obtain a fluorescent carbon dot-linearized polyethyleneimine composite material solution;
c. b, adding the linear polyethylene to the fluorescent carbon dots obtained in the step bAdding sulfydryl modified si into imine composite material solutionTnfαMixing, siTnfαThe final concentration of the fluorescent carbon dots is 100ng/ml, the reaction is carried out for 20min, and the fluorescent carbon dots-linear polyethyleneimine-si are obtainedTnfαA composite material;
d. the third generation bone marrow mesenchymal stem cells are divided into 1 × 104The density of each well was plated on a cell culture plate at 37 ℃ with 5% CO2Culturing overnight under the condition, removing the culture medium, and adding a serum-free and double-resistant alpha-MEM culture medium;
the method for separating and culturing the mesenchymal stem cells comprises the following steps: repeatedly flushing marrow cavity with stem cell culture medium containing heparin anticoagulant under aseptic condition, collecting marrow suspension, diluting with D-Hanks solution, adding Ficoll separating medium into centrifuge tube, slowly adding diluted marrow suspension onto Ficoll separating medium, centrifuging at 2000 rpm/min for 10 min, collecting boundary layer cells rich in nucleated cells, cleaning with D-Hanks solution, and adjusting cell concentration to 1 × 104Perml inoculated in a culture flask at 37 ℃ with 5% CO2Carrying out standing culture under the saturated humidity condition;
the passage method of the bone marrow mesenchymal stem cells comprises the following steps: the cells grow to 90% of the bottom of the bottle for passage, the cells are washed by sterile PBS, then 0.25% trypsin solution is added, digestion is stopped after 1min, the suspended cells are blown, the suspension is collected, centrifugation is carried out at 1500rpm/min for 5min, and the supernatant is discarded; suspending the cells in a culture flask by using a complete culture medium, and carrying out passage once every 2 days;
e. c, the fluorescent carbon dots-linearized polyethyleneimine-si obtained in the step cTnfαThe composite material is added to a cell culture plate containing bone marrow mesenchymal stem cells, and a complete culture medium is replaced after transfection.
Example 2
Carbon dot mediated siTnfαA method for regulating and controlling inflammation to promote mesenchymal stem cell cartilage differentiation comprises the following steps:
a. mixing the fluorescent carbon dots with a PBS solution, wherein the concentration is 250 ug/mL; adding linear polyethyleneimine into the fluorescent carbon dot solution, wherein the addition amount of the linear polyethyleneimine is 2/5 of the volume of the fluorescent carbon dot solution, and stirring the mixed solution at 30 ℃ for 1.5 h;
b, centrifuging at 8000rpm/min for 3min, adding the centrifuged supernatant into a desalting column, eluting by adopting MES solution and filtering by adopting a 0.22-micron membrane to obtain a fluorescent carbon dot-linearized polyethyleneimine composite material solution;
c. adding sulfydryl modified si into the fluorescent carbon dot-linear polyethyleneimine composite material solution obtained in the step bTnfαMixing, siTnfαThe final concentration of the fluorescent carbon dots is 100ng/ml, the reaction is carried out for 40min, and the fluorescent carbon dots-linear polyethyleneimine-si are obtainedTnfαA composite material;
d. the third generation bone marrow mesenchymal stem cells are divided into 1 × 105The density of each well was plated on a cell culture plate at 37 ℃ with 5% CO2Culturing overnight under the condition, removing the culture medium, and adding a serum-free and double-resistant alpha-MEM culture medium;
the method for separating and culturing the mesenchymal stem cells comprises the following steps: repeatedly flushing marrow cavity with stem cell culture medium containing heparin anticoagulant under aseptic condition, collecting marrow suspension, diluting with D-Hanks solution, adding Ficoll separating medium into centrifuge tube, slowly adding diluted marrow suspension onto Ficoll separating medium, centrifuging at 1500rpm/min for 20min, collecting boundary layer cells rich in nucleated cells, cleaning with D-Hanks solution, and adjusting cell concentration to 1 × 105Perml inoculated in a culture flask at 37 ℃ with 5% CO2Carrying out standing culture under the saturated humidity condition;
the passage method of the bone marrow mesenchymal stem cells comprises the following steps: the cells grow to 80% of the bottom of the bottle for passage, the cells are washed by sterile PBS, then 0.25% trypsin solution is added, digestion is stopped after 2min, the suspended cells are blown, the suspension is collected, centrifugation is carried out at 1200rpm/min for 8min, and the supernatant is discarded; suspending the cells in a culture flask by using a complete culture medium, and carrying out passage once in 3 days;
e. c, the fluorescent carbon dots-linearized polyethyleneimine-si obtained in the step cTnfαThe composite material is added to a cell culture plate containing bone marrow mesenchymal stem cells, and a complete culture medium is replaced after transfection.
Example 3
Carbon dot mediated siTnfαA method for regulating and controlling inflammation to promote mesenchymal stem cell cartilage differentiation comprises the following steps:
a. mixing the fluorescent carbon dots with a PBS solution, wherein the concentration is 200 ug/mL; adding linear polyethyleneimine into the fluorescent carbon dot solution, wherein the addition amount of the linear polyethyleneimine is 3/10 of the volume of the fluorescent carbon dot solution, and stirring the mixed solution at 35 ℃ for 1 h;
b. centrifuging at 7000rpm/min for 4min, adding the centrifuged supernatant into a desalting column, eluting by adopting MES solution, and filtering by adopting a 0.22-micrometer membrane to obtain a fluorescent carbon dot-linearized polyethyleneimine composite material solution;
c. adding sulfydryl modified si into the fluorescent carbon dot-linear polyethyleneimine composite material solution obtained in the step bTnfαMixing, siTnfαThe final concentration of the fluorescent carbon dots is 100ng/ml, the reaction is carried out for 30min, and the fluorescent carbon dots-linear polyethyleneimine-si are obtainedTnfαA composite material;
d. the third generation bone marrow mesenchymal stem cells are divided into 1 × 105The density of each well was plated on a cell culture plate at 37 ℃ with 5% CO2Culturing overnight under the condition, removing the culture medium, and adding a serum-free and double-resistant alpha-MEM culture medium;
the method for separating and culturing the mesenchymal stem cells comprises the following steps: repeatedly flushing marrow cavity with stem cell culture medium containing heparin anticoagulant under aseptic condition, collecting marrow suspension, diluting with D-Hanks solution, adding Ficoll separating medium into centrifuge tube, slowly adding diluted marrow suspension onto Ficoll separating medium, centrifuging at 1800rpm/min for 15 min, collecting boundary layer cells rich in nucleated cells, cleaning with D-Hanks solution, and adjusting cell concentration to 1 × 105Perml inoculated in a culture flask at 37 ℃ with 5% CO2Carrying out standing culture under the saturated humidity condition;
the passage method of the bone marrow mesenchymal stem cells comprises the following steps: the cells grow to 85% of the bottom of the bottle for passage, the sterile PBS is used for washing the cells, then 0.25% trypsin liquid is added, digestion is stopped after 1.5min, the suspended cells are blown, the suspension is collected, centrifugation is carried out for 7min at 1300rpm/min, and the supernatant is discarded; suspending the cells in a culture flask by using a complete culture medium, and carrying out passage once in 3 days;
e. c, the fluorescent carbon dots-linearized polyethyleneimine-si obtained in the step cTnfαThe composite material is added to a cell culture plate containing bone marrow mesenchymal stem cells and is further transfectedThe complete medium was changed.
Articular cartilage defect repair score detection
The samples of examples 1 to 3 and untreated mesenchymal stem cells (control group) were injected into Cartilage-deficient rats at 100ul, and articular Cartilage defect Repair was scored according to the International Cartilage Repair Society (ICRS) scoring system, and the data was processed and analyzed.
The experimental results show that the difference between examples 1-3 and the control group was statistically significant at 4 and 8 weeks post-surgery (P < 0.05). Therefore, the invention has good effect on repairing articular cartilage defect.
Claims (7)
1. Carbon dot mediated siTnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation is characterized in that: the method comprises the following steps:
a. mixing the fluorescent carbon dots with a PBS solution, wherein the concentration is 150-250 ug/mL; adding linear polyethyleneimine into the fluorescent carbon dot solution, and stirring the mixed solution at 30-40 ℃ for 0.5-1.5 h;
b. 6000-;
c. adding si into the fluorescent carbon dot-linear polyethyleneimine composite material solution obtained in the step bTnfαMixing, reacting for 20-40min to obtain fluorescent carbon dot-linear polyethyleneimine-siTnfαA composite material;
d. the third generation bone marrow mesenchymal stem cells are divided into 1 × 104-105The density of each well was plated on a cell culture plate at 37 ℃ with 5% CO2Culturing overnight under the condition, removing the culture medium, and adding a serum-free and double-resistant alpha-MEM culture medium;
e. c, the fluorescent carbon dots-linearized polyethyleneimine-si obtained in the step cTnfαThe composite material is added to a cell culture plate containing bone marrow mesenchymal stem cells, and a complete culture medium is replaced after transfection.
2. The carbon dot-mediated si according to claim 1TnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation is characterized in that: in the step a, the addition amount of the linearized polyethyleneimine is 1/5-2/5 of the volume of the fluorescent carbon dot solution.
3. The carbon dot-mediated si according to claim 1TnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation is characterized in that: in the step b, the fluorescent carbon dot-linearized polyethyleneimine composite material solution is filtered by adopting a 0.22 mu m membrane.
4. The carbon dot-mediated si according to claim 1TnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation is characterized in that: in step c, siTnfαThe final concentration of (a) is 100 ng/ml.
5. The carbon dot-mediated si according to claim 1TnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation is characterized in that: in step c, siTnfαSelecting Si modified by sulfydrylTnfα。
6. The carbon dot-mediated si according to claim 1TnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation is characterized in that: in the step d, the method for separating and culturing the mesenchymal stem cells comprises the following steps: repeatedly flushing a marrow cavity with a stem cell culture medium containing heparin anticoagulant under aseptic conditions, collecting marrow suspension, diluting with D-Hanks liquid, adding Ficoll separating medium into a centrifuge tube, slowly adding the diluted marrow suspension onto the Ficoll separating medium, centrifuging at 1500-2000 rpm/min for 10-20 min, collecting boundary layer cells rich in nucleated cells, cleaning with D-Hanks liquid, and adjusting the cell concentration to 1 × 104-105Perml inoculated in a culture flask at 37 ℃ with 5% CO2And carrying out standing culture under the saturated humidity condition.
7. The carbon dot-mediated si according to claim 1TnfαA method for regulating and controlling inflammation and promoting mesenchymal stem cell cartilage differentiation is characterized in that: in the step d, the passage method of the bone marrow mesenchymal stem cells is as follows: the cells grow to 80-90% of the bottom of the bottle for passage, the cells are washed by sterile PBS and then added with 0.25% trypsin solution, digestion is stopped after 1-2min, suspension cells are blown, suspension is collected, centrifugation is carried out at 1200-1500rpm/min for 5-8min, and supernatant is discarded; cells were suspended in culture flasks using complete medium and passaged once every 2-3 days.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062964A (en) * | 2015-07-17 | 2015-11-18 | 山东大学 | Inducing liquid for improving osteogenic differentiation efficiency in stem cells and application of inducing liquid |
| CN109207143A (en) * | 2018-11-16 | 2019-01-15 | 浙江大学医学院附属邵逸夫医院 | A kind of fluorescent carbon quantum dot of functional modification and its preparation method and application |
| US20190032050A1 (en) * | 2015-03-09 | 2019-01-31 | University Of Kentucky Research Foundation | Rna nanoparticle for treatment of gastric cancer |
-
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- 2021-11-25 CN CN202111411898.8A patent/CN114107215A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190032050A1 (en) * | 2015-03-09 | 2019-01-31 | University Of Kentucky Research Foundation | Rna nanoparticle for treatment of gastric cancer |
| CN105062964A (en) * | 2015-07-17 | 2015-11-18 | 山东大学 | Inducing liquid for improving osteogenic differentiation efficiency in stem cells and application of inducing liquid |
| CN109207143A (en) * | 2018-11-16 | 2019-01-15 | 浙江大学医学院附属邵逸夫医院 | A kind of fluorescent carbon quantum dot of functional modification and its preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| JIANWEI LIU 等: ""Bioconjugated Carbon Dots for Delivery of siTnfα to Enhance Chondrogenesis of Mesenchymal Stem Cells by Suppression of Inflammation"", 《TEM CELLS TRANSLATIONAL MEDICINE》, vol. 8, pages 4 - 6 * |
| QING WANG 等: ""Fluorescent carbon dots as an efficient siRNA nanocarrier for its interference therapy in gastric cancer cells"", 《OURNAL OF NANOBIOTECHNOLOGY》, vol. 12, no. 58, pages 3 * |
| 董悦;郑嵘;布文奂;刘杰;余彦衡;高旭广;孙宏晨;吴立鹏;: "碳点在成骨细胞系内跨膜转运机制的研究", 北京口腔医学, vol. 25, no. 2, pages 71 - 75 * |
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