CN114107063A - Aspergillus fumigatus strain and application thereof - Google Patents

Aspergillus fumigatus strain and application thereof Download PDF

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CN114107063A
CN114107063A CN202110988282.0A CN202110988282A CN114107063A CN 114107063 A CN114107063 A CN 114107063A CN 202110988282 A CN202110988282 A CN 202110988282A CN 114107063 A CN114107063 A CN 114107063A
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phosphorus
aspergillus fumigatus
coal gangue
potassium
calcium
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谢承卫
吉俐
李小军
盛定红
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China Tobacco Corp Guizhou Provincial Co
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Abstract

The invention discloses an aspergillus fumigatus strain and application thereof. The Aspergillus fumigatus strain is preserved in China Center for Type Culture Collection (CCTCC) at 25.06.2021, with the preservation number of M2021784, and is classified and named as Aspergillus fumigatus GZT-Asp 01. The aspergillus fumigatus GZT-Asp01 can dissociate phosphorus, potassium, calcium, nitrogen and other components in coal gangue and convert the phosphorus, potassium, calcium, nitrogen and other components into nutrient components which can be absorbed by plants, such as available phosphorus, quick-acting potassium, exchangeable calcium, hydrolyzed nitrogen and the like, particularly can dissociate insoluble phosphorus and/or insoluble phosphorus in the coal gangue effectively, and the phosphorus solubilizing effect of the aspergillus fumigatus GZT-Asp01 on the coal gangue is superior to that of the traditional phosphorus solubilizing strains such as bacillus megaterium.

Description

Aspergillus fumigatus strain and application thereof
Technical Field
The invention relates to the technical field of microorganisms, in particular to an aspergillus fumigatus strain and application thereof.
Background
China is a large coal producing country, and coal occupies an important position in the energy structure of China. A large amount of coal gangue is produced in the coal mining and washing processes, the yield of the coal gangue accounts for 20% -35% of the yield of the coal, and the coal gangue is not effectively utilized for a long time and becomes the largest industrial solid waste. The coal gangue produced in each coal producing area is basically stored in an open-air accumulation mode, and the large accumulation of the open-air coal gangue causes a plurality of hazards: the coal gangue dump occupies a large amount of land resources; toxic gas is released by spontaneous combustion of weathered coal gangue, dust is generated, and heavy metal pollution is caused; the rainwater erodes the coal gangue, so that the components of the coal gangue are changed, toxic substances are generated and flow into the soil, and the water quality is polluted. In a word, the accumulation of a large amount of coal gangue causes resource waste and serious environmental pollution, so that the development of a new coal gangue resource utilization method has important significance.
The component analysis of the coal gangue shows that the coal gangue contains a plurality of nutrient components required by plant growth, such as: phosphorus, potassium, calcium, nitrogen, organic matter, etc., but most of the components contained in the coal gangue, such as phosphorus and potassium, exist in the form of insoluble minerals and are not easily utilized effectively. If the components can be effectively utilized, the pressure of the coal gangue on the environment can be relieved to a great extent.
Disclosure of Invention
The invention aims to provide an aspergillus fumigatus strain and application thereof. The aspergillus fumigatus GZT-Asp01 can dissociate phosphorus, potassium, calcium, nitrogen and other components in coal gangue and convert the phosphorus, potassium, calcium, nitrogen and other components into nutrient components which can be absorbed by plants, such as available phosphorus, quick-acting potassium, exchangeable calcium, hydrolyzed nitrogen and the like, and particularly can effectively dissociate insoluble phosphorus and/or insoluble phosphorus components in the coal gangue. The technological process for dissociating the coal gangue by adopting the strain is simple, the dissociation effect is obvious, the cost is low, and the method is green and environment-friendly.
The technical scheme of the invention is as follows: an Aspergillus fumigatus strain is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2021784 at 25.06.2021, and is classified and named as Aspergillus fumigatus GZT-Asp 01.
An application of Aspergillus fumigatus strain in dissociating P, K, Ca and N in coal gangue is disclosed.
A product for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue, wherein the active ingredient of the product comprises the Aspergillus fumigatus strain.
A product for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue, wherein the active ingredient of the product is the aspergillus fumigatus strain.
A preparation method of a product for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue adopts the aspergillus fumigatus strain as an active ingredient for preparing the product.
A preparation method of a product for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue adopts the aspergillus fumigatus strain as one of active ingredients for preparing the product.
A microbial inoculum for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue comprises the aspergillus fumigatus strain as an active ingredient.
A microbial inoculum for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue, wherein the active ingredient of the microbial inoculum is the aspergillus fumigatus strain.
A method for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue, the aspergillus fumigatus strain is obtained in the dissociation process.
A method for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue uses any product or microbial inoculum obtained in the dissociation process.
The Aspergillus fumigatus strains are deposited, and the preservation information is as follows:
the deposit unit code: CCTCC (China center for cell communication)
Address: university of Wuhan, China
Whether survival is carried out: is that
The preservation date is as follows: 25/06/2021
The preservation number is: CCTCC NO: M2021784
And (3) classification and naming: aspergillus fumigatus GZT-Asp01
Aspergillus fumigatiaffinis strain GZT-Asp01
The invention has the advantages of
1. The aspergillus fumigatus strain GZT-Asp01(Aspergillus fumigatus strain) is a strain which can effectively convert insoluble phosphorus, potassium, calcium, nitrogen and the like in the coal gangue into effective phosphorus, quick-acting potassium, exchangeable calcium, hydrolyzed nitrogen and the like which are used as nutrient components absorbed by plants, particularly can effectively dissociate insoluble phosphorus and/or insoluble phosphorus in the coal gangue, and has a phosphorus solubilizing effect on the coal gangue superior to that of the traditional phosphorus solubilizing strain such as Bacillus megaterium (Bacillus megaterium).
2. The process for dissociating the coal gangue by adopting the aspergillus fumigatus strain GZT-Asp01 is simple, low in cost and environment-friendly.
3. The method for dissociating the coal gangue by using the aspergillus fumigatus strain GZT-Asp01 is an effective method for recycling the coal gangue, can relieve the pressure of the coal gangue on the environment, can prepare the microbial fertilizer by using the microbial fungi to dissociate the coal gangue, can convert insoluble and/or insoluble components in the coal gangue into soluble components, can dissociate and dissolve the solidified phosphorus resource in the soil into phosphorus which can be absorbed and utilized by plants after being applied to the soil, can improve the phosphorus absorption coefficient of the soil, and can improve the capability of the soil for absorbing and fixing the phosphorus. Compared with the traditional chemical method for processing the coal gangue, the method has the advantage of environmental friendliness.
4. The Aspergillus fumigatus strain GZT-Asp01 can not only dissociate coal gangue, but also effectively dissociate other phosphorus-containing minerals.
Drawings
FIG. 1 is a growth curve of the GZT-Asp01 strain according to the invention;
FIG. 2 is a diagram showing the appearance of colonies of the GZT-Asp01 strain according to the present invention;
FIG. 3 is a microscopic morphology of GZT-Asp01 cells according to the present invention;
FIG. 4 shows a phylogenetic tree (Neighbor-Joining phylogenetic tree constructed based on the alignment result of 18S rRNA gene sequences and EF024313.1 as an outgrowth) established by the GZT-Asp01 strain based on the 18S rRNA gene sequences.
Detailed Description
The present invention is further illustrated by the following examples, which are not to be construed as limiting the invention.
The embodiment of the invention comprises the following steps:
example 1: isolation and screening of functional strains
1) Collection of samples
(1) The sample is collected from coal gangue dump in a coal producing area of Guizhou and soil nearby. And (3) selecting plant root soil with good growth vigor, shoveling off the soil on the ground surface, digging 10-20 cm along the plant roots, and collecting the soil in a sterile self-sealing bag by adopting a root shaking method.
(2) The ice box is sealed and taken back to the laboratory, and after plant residues are removed, the ice box is crushed and sieved by a 20-mesh sieve.
(3) The samples were stored in a refrigerator at 4 ℃ for cold storage.
2) Screening of functional strains
(1) 1g of the sample was accurately weighed into a sterilized 250mL Erlenmeyer flask, and 99mL of sterile water was added.
(2) Oscillating for 30min in a constant temperature shaking table at about 28 ℃ at 170 r/min.
(3) Oscillating for 30min, and gradually diluting the supernatant to 1.0 × 10-3，1.0×10-4，1.0×10-5， 1.0×10-6，1.0×10-7。
(4) 100uL of each concentration is coated on 3 phosphate solubilizing bacteria screening solid culture mediums.
(5) And (3) placing the flat plate in a constant temperature incubator at 30 ℃ for culturing for 3-7 days.
(6) Inoculating single colony points with different appearance characteristics on phosphate solubilizing bacteria screening solid culture.
(7) And after the strain grows out, observing the purification condition of the strain through colony appearance morphology observation and fungus microscopic morphology observation, and repeatedly performing purification culture for many times until a single strain is obtained if the purification requirement is not met.
(8) The purified strain was stained with safranin.
(9) Culturing the primarily screened purified strain, dissociating the coal gangue, and determining the phosphorus dissolving effect of the strain by measuring the change condition of the effective phosphorus content before and after dissociation of the coal gangue.
Example 2: drawing of growth curves of functional strains
1) Preparing a fungal spore suspension: picking fungus hypha to be detected on a PDA slant culture medium by using an inoculating needle, activating for 36-72 h at 30 ℃, taking out after a large amount of fungus spores grow out, washing the slant with 5mL of sterile distilled water on a super clean workbench to prepare fungus spore suspension, diluting to 100mL, and shaking uniformly for later use.
2) Inoculation and culture: pouring 100mL LB liquid culture medium into 250mL conical flask, preparing 30 bottles in total, sterilizing at 121 deg.C for 30min in autoclave, inoculating 5% fungal spore suspension into sterilized LB liquid culture medium, and culturing at 170 r.min-1Shaking culture at 30 deg.C. The strain number and the incubation time were marked on the Erlenmeyer flask.
3) And (3) determination: taking out 3 bottles every 24h, drying the blank filter paper to constant weight at 105 ℃, recording the weight, filtering the bacterial liquid, drying the bacterial liquid again to constant weight, recording the weight, wherein the weight difference of two times is the dry weight of the hyphae, and the unit of the result is expressed by mg.
The growth curve of the GZT-Asp01 fungus, measured by the dry weight method, is shown in FIG. 1: the logarithmic growth phase of the GZT-Asp01 fungi is 4-6 days, and the strain reaches the stationary phase after 6 days.
Example 3: identification of functional strains
The fungus GZT-Asp01 is assigned to China center for type culture Collection for identification.
1) Appearance morphology of colony
The primary hyphae of the GZT-Asp01 fungus are white and hairy, the middle of the colony protrudes and spreads radially, after 5 days, the middle of the colony begins to appear dark green spores, the hyphae can grow over the whole flat plate for about 9 days, and the culture medium matrix can be seen to turn yellow brown from the back of the flat plate, as shown in figure 2.
2) Microscopic morphology of fungi
The fungus GZT-Asp01 was stained by safranin staining, and microscopic morphology of the fungus was observed under an optical microscope after staining. The optical microscope can observe that the GZT-Asp01 fungi hypha is transparent, the top of the conidiophore expands to form a top sac which is hemispherical, spherical conidia are generated on the surface of the top sac, and the conidia are transparent and spherical, as shown in figure 3.
3) ITS rRNA gene sequence:
GGGGCTCGAGTGAGACTCTGGGTCCACCTCCCACCCGTGTCTATC GTACCTTGTTGCTTCGGCGGGCCCGCCGTTTCGACGGCCGCCGGGGAG GCCTCGCGCCCCCGGGCCCGCGCCCGCCGAAGACCCCAACATGAACT CTGTTCTGAAAGTATGCAGTCTGAGTTGATTATCATAATCAGTTAAAAC TTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGA AATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTT TGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGA GCGTCATTGCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCCGTCCC CGGTTTCCCCCGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTC CGGTCCTCGAGCGTATGGGGCTTTGTCACCCGCTCTGTAGGCCCGGCC GGCGCCAGCCGACACCCAACTTTATTTCTAAGGTTGACCTCGGATCAG GTAGGGATACCCGCTGAACTTAAGCATATCATAAAGGCCGGAGGA
4)18S rRNA gene sequence:
TATCTGATGTCTAGTATAAGCAATTTATACGGTGAAACTGCGAATG GCTCATTAAATCAGTTATCGTTTATTTGATAGTACCTTACTACATGGATAC CTGTGGTAATTCTAGAGCTAATACATGCTAAAAACCTCGACTTCGGAAG GGGTGTATTTATTAGATAAAAAACCAATGCCCTTCGGGGCTCCTTGGTG AATCATAATAACTTAACGAATCGCATGGCCTTGCGCCGGCGATGGTTCA TTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCAT GGTGGCAACGGGTAACGGGGAATTAGGGTTCGATTCCGGAGAGGGAG CCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAAT TACCCAATCCCGACACGGGGAGGTAGTGACAATAAATACTGATACGGG GCTCTTTTGGGTCTCGTAATTGGAATGAGTACAATCTAAATCCCTTAAC GAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTC CAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGT TGAACCTTGGGTCTGGCTGGCCGGTCCGCCTCACCGCGAGTACTGGTC CGGCTGGACCTTTCCTTCTGGGGAACCTCATGGCCTTCACTGGCTGTG GGGGGAACCAGGACTTTTACTGTGAAAAAATTAGAGTGTTCAAAGCA GGCCTTTGCTCGAATACATTAGCATGGAATAATAGAATAGGACGTGCGG TTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTC GGGGGCGTCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTG AAGACTAACTACTGCGAAAGCATTCGCCAAGGATGTTTTCATTAATCAG GGAACGAAAGTTAGTGGATCGAAGACGATCAGATACCGTCGTAGTCTT AACCATAAACTATGCCGACTAGGGATCGGGCGGTGTTTCTATGATGACC CGCTCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTCTGGGGGGAGT ATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACA AGGCGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACC AGGTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATC TTTTGGATGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCT GCTTAATTGCGATAACGAACGAGACCTCGGCCCTTAAATAGCCCGGTC CGCATTTGCGGGCCGCTGGCTTCTTAGGGGGACTATCGGCTCAAGCCG ATGGAAGTGCGCGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTG GGCCGCACGCGCGCTACACTGACAGGGCCAGCGAGTACATCACCTTG GCCGAGAGGTCTGGGTAATCTTGTTAAACCCTGTCGTGCTGGGGATAG AGCATTGCAATTATTGCTCTTCAACGAGGAATGCCTAGTAGGCACGAGT CATCAGCTCGTGCCGATTACGTCCCTGCCCTTTGTACACACCGCCCGTC GCTACTACCGATTGAATGGCTCGGTGAGGCCTTCGGACTGGCTCAGGG GAGTTGGCAACGACTCCCCAGAGCCGGAAAGTTGGTCAAACCCGGTC ATTAGAGGAAGTAAAGTGTTG
5)28S rRNA gene sequence:
CCTCGGGCATTGCCTCGTACGGCGAGTGAAGCGGCAAGAGCTCA AATTTGAAAGCTGGCCCCTTCGGGGTCCGCGTTGTAATTTGCAGAGGA TGCTTCGGGTGCAGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCATA GAGGGTGAGAATCCCGTCTGGGACGGGGTGTCTGCGTCCGTGTGAAG CTCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGG TAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAA GTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACA GCACGTGAAATTGTTGAAAGGGAAGCGTTTGCGACCAGACTCGCTCG CGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGTGGGCGGGCC AGCGTCGGTTTGGGCGGCCGGTCAAAGGCCCTCGGAATGTATCACCTC TCGGGGTTGTCTTATAGCCGAGGGTGCAATGCGGCCTGCCCGGACCGA GGAACGCGCTTCGGCTCGGACGCTGGCGTAATGGTCGTAAATGACCCG TCTTAAACAACCCGAACCAA
6) physiological and biochemical characteristics of the strain:
TABLE 1 carbon source Oxidation of the strains
Detecting items Results Detecting items Results
A1 Water (W) - B7 D-galactose -
A2 Tween 80 + B8 D-galacturonic acid +
A3 N-acetyl-galactosamine + B9 Gentiobiose +
A4 N-acetyl-glucosamine + B10 D-gluconic acid +
A5 N-acetyl-mannosamine + B11 D-glucosamine +
A6 Ribitol - B12 alpha-D-glucose +
A7 Amygdalin + C1 1-phosphoric acid-glucose +
A8 D-arabinose - C2 Glucuronamide -
A9 L-arabinose + C3 D-glucuronic acid +
A10 D-arabinitol + C4 Glycerol +
A11 Arbutin + C5 Glycogen +
A12 D-Cellobiose + C6 M-inositol +
B1 Alpha-cyclodextrin + C7 2-keto-D-gluconic acid +
B2 Beta-cyclodextrin + C8 alpha-D-lactose +
B3 Glucan + C9 Lactulose +
B4 I-Red-silk algae alcohol + C10 Maltitol +
B5 D-fructose + C11 Maltose +
B6 L-fucose + C12 Maltotriose +
Note: in table 1, +: positive reaction; -: and (4) carrying out negative reaction.
TABLE 2 carbon Source utilization by the strains
Detecting items Results Detecting items Results
D1 D-mannitol + F7 P-o-hydroxyphenylacetic acid -
D2 D-mannose + F8 Alpha-ketoglutaric acid -
D3 D-melezitose + F9 D-lactic acid methyl ester +
D4 D-melibiose + F10 L-lactic acid +
D5 alpha-methyl-D-galactosides - F11 D-malic acid -
D6 beta-methyl-D-galactosides + F12 L-malic acid +
D7 alpha-methyl-D-glucoside + G1 D-glucaric acid -
D8 beta-methyl-D-glucoside + G2 Sebacic acid +
D9 Isomaltulose + G3 Succinamic acid +
D10 D-psicose + G4 Succinic acid +
D11 D-raffinose + G5 Succinic acid monomethyl ester -
D12 L-rhamnose + G6 N-acetyl-L-glutamic acid -
E1 D-ribose + G7 Propionic acid amine +
E2 Salicin + G8 L-alanine +
E3 Crassulaceae aldehydePolysaccharides + G9 L-alanylglycine -
E4 D-sorbitol + G10 L-asparagine +
E5 L-sorbose - G11 L-aspartic acid -
E6 Stachyose + G12 L-glutamic acid +
E7 Sucrose + H1 L-glycyl glutamic acid +
E8 D-tagatose + H2 L-ornithine +
E9 D-trehalose + H3 L-phenylalanine -
E10 Turanose + H4 L-proline +
E11 Xylitol, its preparation method and use + H5 L-pyroglutamic acid +
E12 D-xylose + H6 L-serine +
F1 Gamma-butylamine acid + H7 L-threonine +
F2 Bromocutanedioic acid + H8 2-ethanolamine +
F3 Fumaric acid + H9 Putrescine -
F4 Beta-hydroxyisobutyric acid + H10 Adenosine (I) -
F5 Gamma-hydroxyisobutyric acid - H11 Uridine (uridine) -
F6 L-rhamnose + H12 Adenine nucleotide -
Note: in table 2, +: positive reaction; -: and (4) carrying out negative reaction.
Example 4: determination of dissociation capability of strain to coal gangue
1) The coal gangue comprises the following main components: c10.52%, SiO2 38.71％，Al2O3 16.30％， Fe2O316.11％，TiO2 4.30％，CaO 4.25％，MgO 2.03％，K2O 1.51％，Na2O 0.88％， P2O51.78%, S1.34%, H1.10%, N0.86%, ash 82.21%.
2) The four main factors affecting coal gangue dissociation are: inoculation amount, gangue particle size, dissociation time and system pH value. Firstly, a single-factor experiment of dissociating coal gangue by GZT-Asp01 is carried out, the optimal single-factor condition of each bacterium is searched, and then the optimal condition of dissociating the coal gangue by GZT-Asp01 is searched by a design orthogonal experiment.
3) The dissociation experiment was carried out with the fungus GZT-Asp01 for the coal gangue ingredient 1).
4) The dissociation experiment procedure was as follows: taking the coal gangue with the specified mesh number, putting the coal gangue in a temperature-controlled stirrer, and controlling the concentration of GZT-Asp01 bacterial liquid to be: 1.0X 1010-1.5×1010The ratio of the amount of the two strains to the coal gangue is 1:1 (volume: mass), the bacteria liquid is sprayed under continuous stirring, the mixture is stirred evenly, the mixture is turned over once every 4 to 6 hours, the temperature is controlled at 30 ℃, and the coal gangue is dissociated for several days.
The culture method of the GZT-Asp01 bacterial liquid comprises the following steps:
firstly, under the aseptic condition, the aspergillus fumigatus GZT-Asp01 strain is placed at room temperature for thawing.
Secondly, after thawing, shaking up under aseptic condition, sucking GZT-Asp01 bacterial liquid into PDA solid culture medium, coating and spreading evenly, and culturing in a constant temperature incubator at 30 ℃ for 5-7 d.
Thirdly, the bacterial strain obtained by the last step is selected by an inoculating loop under the aseptic condition, and is inoculated in an LB solid culture medium by a line drawing method, and is cultured for 5-7 days in a constant temperature incubator at 30 ℃.
Fourthly, after the aspergillus fumigatus GZT-Asp01 grows well, eluting the fungus growing well in the culture medium by using sterile water under the aseptic condition to obtain a bacterial liquid, wherein the concentration range of the bacterial liquid is controlled as follows: 1.0X 1010-1.5×1010cfu/mL。
5) The content of each index of the dissociated coal gangue is measured, and the result is shown in table 3. As can be seen from Table 3, the overall effect of dissociating coal gangue by GZT-Asp01 is better than that of Bacillus megaterium, and the main indexes of available phosphorus, fast potassium and the like are better than that of the Bacillus megaterium.
TABLE 3-orthogonal design Experimental results
Figure BDA0003231545270000101
Figure BDA0003231545270000111
The above description is only for the purpose of illustrating the present invention, and the scope of the present invention is not limited thereto, and any person skilled in the art can substitute or change the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Figure RE-GDA0003455583430000131
Figure RE-GDA0003455583430000141
Figure RE-GDA0003455583430000151
Sequence listing
<110> Guizhou province company, China tobacco general company
<120> A fumagilloensis and uses thereof
<160> 3
<170> SIPOSequenceListing 1.0
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<211> 570
<212> RNA
<213> Aspergillus fumigatus strain
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ggggcucgag ugagacucug gguccaccuc ccacccgugu cuaucguacc uuguugcuuc 60
ggcgggcccg ccguuucgac ggccgccggg gaggccucgc gcccccgggc ccgcgcccgc 120
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caccgcgucc gguccucgag cguauggggc uuugucaccc gcucuguagg cccggccggc 480
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aacggguaac ggggaauuag gguucgauuc cggagaggga gccugagaaa cggcuaccac 360
auccaaggaa ggcagcaggc gcgcaaauua cccaaucccg acacggggag guagugacaa 420
uaaauacuga uacggggcuc uuuugggucu cguaauugga augaguacaa ucuaaauccc 480
uuaacgagga acaauuggag ggcaagucug gugccagcag ccgcgguaau uccagcucca 540
auagcguaua uuaaaguugu ugcaguuaaa aagcucguag uugaaccuug ggucuggcug 600
gccgguccgc cucaccgcga guacuggucc ggcuggaccu uuccuucugg ggaaccucau 660
ggccuucacu ggcugugggg ggaaccagga cuuuuacugu gaaaaaauua gaguguucaa 720
agcaggccuu ugcucgaaua cauuagcaug gaauaauaga auaggacgug cgguucuauu 780
uuguugguuu cuaggaccgc cguaaugauu aauagggaua gucgggggcg ucaguauuca 840
gcugucagag gugaaauucu uggauuugcu gaagacuaac uacugcgaaa gcauucgcca 900
aggauguuuu cauuaaucag ggaacgaaag uuaguggauc gaagacgauc agauaccguc 960
guagucuuaa ccauaaacua ugccgacuag ggaucgggcg guguuucuau gaugacccgc 1020
ucggcaccuu acgagaaauc aaaguuuuug gguucugggg ggaguauggu cgcaaggcug 1080
aaacuuaaag aaauugacgg aagggcacca caaggcgugg agccugcggc uuaauuugac 1140
ucaacacggg gaaacucacc agguccagac aaaauaagga uugacagauu gagagcucuu 1200
ucuugaucuu uuggauggug gugcauggcc guucuuaguu gguggaguga uuugucugcu 1260
uaauugcgau aacgaacgag accucggccc uuaaauagcc cgguccgcau uugcgggccg 1320
cuggcuucuu agggggacua ucggcucaag ccgauggaag ugcgcggcaa uaacaggucu 1380
gugaugcccu uagauguucu gggccgcacg cgcgcuacac ugacagggcc agcgaguaca 1440
ucaccuuggc cgagaggucu ggguaaucuu guuaaacccu gucgugcugg ggauagagca 1500
uugcaauuau ugcucuucaa cgaggaaugc cuaguaggca cgagucauca gcucgugccg 1560
auuacguccc ugcccuuugu acacaccgcc cgucgcuacu accgauugaa uggcucggug 1620
aggccuucgg acuggcucag gggaguuggc aacgacuccc cagagccgga aaguugguca 1680
aacccgguca uuagaggaag uaaaguguug 1710
<210> 3
<211> 589
<212> RNA
<213> Aspergillus fumigatus strain
<400> 3
ccucgggcau ugccucguac ggcgagugaa gcggcaagag cucaaauuug aaagcuggcc 60
ccuucggggu ccgcguugua auuugcagag gaugcuucgg gugcagcccc cgucuaagug 120
cccuggaacg ggccgucaua gagggugaga aucccgucug ggacggggug ucugcguccg 180
ugugaagcuc cuucgaagag ucgaguuguu ugggaaugca gcucuaaaug ggugguaaau 240
uucaucuaaa gcuaaauacu ggccggagac cgauagcgca caaguagagu gaucgaaaga 300
ugaaaagcac uuugaaaaga gaguuaaaca gcacgugaaa uuguugaaag ggaagcguuu 360
gcgaccagac ucgcucgcgg gguucagccg gcauucgugc cgguguacuu ccccgugggc 420
gggccagcgu cgguuugggc ggccggucaa aggcccucgg aauguaucac cucucggggu 480
ugucuuauag ccgagggugc aaugcggccu gcccggaccg aggaacgcgc uucggcucgg 540
acgcuggcgu aauggucgua aaugacccgu cuuaaacaac ccgaaccaa 589

Claims (10)

1. An aspergillus fumigatus strain, characterized by: is preserved in China Center for Type Culture Collection (CCTCC) at 25.06.2021 with the preservation number of M2021784 and is classified and named as Aspergillus fumigatus GZT-Asp 01.
2. Use of an aspergillus fumigatus strain as claimed in claim 1 for the dissociation of phosphorus, potassium, calcium and nitrogen in coal gangue.
3. A product for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: the active ingredient of the article of manufacture comprises an aspergillus fumigatus strain of claim 1.
4. A product for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: the active ingredient of the preparation is an aspergillus fumigatus strain as claimed in claim 1.
5. A preparation method of products for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: use of an Aspergillus fumigatus strain as claimed in claim 1 as an active ingredient in the preparation of said product.
6. A preparation method of products for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: use of an Aspergillus fumigatus strain as claimed in claim 1 as one of the active ingredients in the preparation of said product.
7. A microbial inoculum for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: the active ingredient of the microbial inoculum comprises an aspergillus fumigatus strain as claimed in claim 1.
8. A microbial inoculum for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: the active ingredient of the microbial inoculum is the aspergillus fumigatus strain of claim 1.
9. A method for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: an aspergillus fumigatus strain according to claim 1 is used in the dissociation process.
10. A method for dissociating phosphorus, potassium, calcium and nitrogen in coal gangue is characterized in that: use of a preparation or inoculum obtained according to any one of claims 3 to 4, 7 to 8 in a dissociation process.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR960031592A (en) * 1995-02-24 1996-09-17 심우영 Aspergillus pumigatus mutant strains and production enzymes and methods for producing chitosan- oligosaccharides using the same
CN102533566A (en) * 2011-12-06 2012-07-04 郑州大学 (Aspergillus fumigates)Ty-1 and application of (Aspergillus fumigates)Ty-1
CN104480024A (en) * 2014-12-24 2015-04-01 浙江工业大学 Aspergillus fumigatus having butyl acetatedegradation capacity and applications thereof
CN112094758A (en) * 2020-09-28 2020-12-18 广东省科学院生物工程研究所 Aspergillus fumigatus strain and application thereof in degradation of polyvinyl alcohol
CN113174337A (en) * 2021-05-27 2021-07-27 常州大学 Thermophilic cellulose-degrading aspergillus fumigatus and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR960031592A (en) * 1995-02-24 1996-09-17 심우영 Aspergillus pumigatus mutant strains and production enzymes and methods for producing chitosan- oligosaccharides using the same
CN102533566A (en) * 2011-12-06 2012-07-04 郑州大学 (Aspergillus fumigates)Ty-1 and application of (Aspergillus fumigates)Ty-1
CN104480024A (en) * 2014-12-24 2015-04-01 浙江工业大学 Aspergillus fumigatus having butyl acetatedegradation capacity and applications thereof
CN112094758A (en) * 2020-09-28 2020-12-18 广东省科学院生物工程研究所 Aspergillus fumigatus strain and application thereof in degradation of polyvinyl alcohol
CN113174337A (en) * 2021-05-27 2021-07-27 常州大学 Thermophilic cellulose-degrading aspergillus fumigatus and application thereof

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