CN114099574B - Extraction method of coptis chinensis extract for reducing blood glucose and extract thereof - Google Patents
Extraction method of coptis chinensis extract for reducing blood glucose and extract thereof Download PDFInfo
- Publication number
- CN114099574B CN114099574B CN202010900167.9A CN202010900167A CN114099574B CN 114099574 B CN114099574 B CN 114099574B CN 202010900167 A CN202010900167 A CN 202010900167A CN 114099574 B CN114099574 B CN 114099574B
- Authority
- CN
- China
- Prior art keywords
- extraction
- extract
- coptis
- butanol
- solvent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Diabetes (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Obesity (AREA)
- Alternative & Traditional Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Endocrinology (AREA)
- Emergency Medicine (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of extraction of active ingredients of medicines, and particularly relates to an extraction method of active ingredients of coptis chinensis medicinal materials for reducing blood sugar and application of the extract. Aiming at the problem that the prior art lacks optimization research on the extraction method of the effective components in the specific coptis plants with higher alkaloid content, the technical scheme of the invention is as follows: [1] taking dry powder of the coptis deltoidea medicinal material; [2] sequentially extracting the dry powder in the step (1) by adopting a plurality of solvents with different polarities, wherein the solvents comprise n-butanol; [3] collecting the extracting solution obtained by n-butanol extraction in the step (2), and concentrating to obtain an extract, namely an extract. The invention is used for extracting the active ingredients with the hypoglycemic effect from the coptis chinensis, and the product can be further used for preparing hypoglycemic combined medicines.
Description
Technical Field
The invention belongs to the technical field of extraction of active ingredients of medicines, and particularly relates to an extraction method of active ingredients of coptis chinensis medicinal materials for reducing blood sugar and application of the extract.
Background
The plant of Coptis in Ranunculaceae is perennial herb and distributed in North temperate zone. Ancient literature, ming Yi Bie Ji: mainly treats five cold and heat accumulation, long-term diarrhea, purulent blood, quenching thirst, great convulsion, removing water, promoting bone conduction, regulating stomach and intestine, benefiting gallbladder and treating aphtha. And the new repair herbal records: ' Huanglian, shudao, is thick and bitter in taste and treats thirst as the most; the Jiang Dong is good at treating dysentery, as it is indicated for Lian Zhu. As can be seen, ancient doctors have recognized that coptis has a better "thirst" effect in long-term clinical practice. Phytochemistry and pharmacology research show that alkaloid components in coptis chinensis medicinal materials are main active substances which play a role in reducing blood sugar. In the prior art, few researches are based on the content difference of alkaloid components in coptis chinensis medicinal materials with different basic sources. And lacks research optimized for the extraction method of the effective components in the specific coptis plants with higher alkaloid content.
Wherein, the triangle leaf coptis is mainly distributed in Sichuan Emei and Hongyan area of China. Wild plants are not common in mountain forests growing at an altitude of 1600-2200 m. In the prior art, it has been reported that the coptis deltoidea contains alkaloids such as berberine, coptisine, methylcoptisine, palmate stephanine and the like, and can treat diseases such as acute conjunctivitis, acute bacillary dysentery, acute gastroenteritis, hematemesis, carbuncle furuncle and sores. However, reports on the hypoglycemic effect of coptis deltoidea are lacking at present.
Disclosure of Invention
Aims at solving the problems that the prior art lacks the content difference of alkaloid components in coptis medicinal materials with different basic sources and lacks the extraction optimization research of effective components in specific coptis plants with higher alkaloid content. The invention provides an extraction method of coptis chinensis medicinal active ingredients for reducing blood sugar and application of the extract. The aim is that: preferably, the type of rhizoma coptidis medicinal materials is optimized, and the extraction method is optimized, so that the hypoglycemic effect of the rhizoma coptidis medicinal material extract is enhanced.
An extraction method of active ingredients of coptis chinensis medicinal materials for reducing blood sugar comprises the following steps:
[1] taking dry powder of the coptis deltoidea medicinal material;
[2] sequentially extracting the dry powder in the step (1) by using petroleum ether, ethyl acetate and n-butanol solvent; the three solvents are solvents with different polarities, so that different active ingredients can be extracted by different solvents when the coptis deltoidea is extracted;
[3] collecting the extracting solution of the n-butanol part in the step (2), concentrating to obtain an extract, namely the extract.
Preferably, the Coptis deltoidea is a dried rhizome of Coptis deltoidea of Coptis genus of Ranunculaceae family; preferably, the content of the granadine in the coptis deltoidea is higher than that of other coptis plants.
Preferably, the preparation method of the dry powder in the step [1] comprises the following steps: taking rhizome of coptis deltoidea, cleaning, drying in air, drying to constant weight, and grinding into 100 mesh powder.
Preferably, the petroleum ether in step [2] has a boiling range of 60 to 90.
Preferably, the extraction in step [2] is performed using a rapid solvent extractor.
Further preferably, step [2] specifically comprises the steps of:
2-1, sequentially pumping a plurality of solvents with different polarities into a rapid solvent extractor to prepare extraction sites with different polarities, wherein the solvents comprise n-butanol;
2-2, arranging a gasket and glass fiber filter paper at the bottom of an extraction tank of a rapid solvent extractor in sequence, then placing a sample into the extraction tank, and then filling quartz sand;
and 2-3, starting a solvent extractor for extraction.
It is further preferable that the mass of the dry powder of the Coptis deltoidea medicinal material added to the extraction tank in the step [2-2] is 0.5-2.0g, the specification of the extraction tank is 40ml, and the amount of quartz sand added to the extraction tank is 40-60g.
It is further preferred that in step [2-3], the extraction is carried out at a temperature of 90℃and a pressure of 100bar.
Further preferably, in the step [2-3], n-butanol is circulated four times as an extraction solvent, wherein the 1 st circulation process is heating up for 1-2min, maintaining for 0min and releasing for 5min, and the 2 nd circulation process to 4 th circulation process is heating up for 1-2min, maintaining for 2-4min and releasing for 5min; the extraction solvent except n-butanol is circulated three times, and each circulation process is heating for 1-2min, maintaining for 2-4min and releasing for 5min.
Further preferably, in the step [2-3], the solvent flushing time after the completion of the extraction of each extraction solvent is 2min, and the gas flushing time is 3min.
The invention also provides an extract obtained by the extraction method.
The invention also provides application of the extract of the coptis chinensis medicinal active ingredient for reducing blood sugar in preparing a blood sugar-reducing pharmaceutical composition.
After the technical scheme of the invention is adopted, the extract obtained by extracting the coptis deltoidea with n-butanol is selected. According to the result of the alpha-glucosidase inhibition experiment, compared with other types of coptis chinensis medicinal materials or extracts obtained by extracting coptis chinensis with other solvents, the extract has more excellent alpha-glucosidase inhibition activity, and thus has better value as a hypoglycemic medicament or for preparing hypoglycemic combined medicaments. According to the results of ultrafiltration rapid screening experiments and molecular docking simulation, the extract of the n-butanol part of the coptis deltoidea contains magnolol, granadine, coptisine, berberine and other alkaloids as potential alpha-glucosidase inhibitors. Especially, the content of the granadine in the coptis deltoidea is obviously higher than that of other three coptis chinensis medicinal materials, which further enhances the alpha-glucosidase inhibition effect of the n-butanol part extract of the coptis deltoidea. Furthermore, the invention also provides an optimization scheme of various technological parameters during extraction of the active ingredients in the coptis chinensis with the trigonal leaves, so that the extraction rate can be further improved, and the content of the active ingredients in the extract can be improved.
It should be apparent that, in light of the foregoing, various modifications, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
The above-described aspects of the present invention will be described in further detail below with reference to specific embodiments in the form of examples. It should not be understood that the scope of the above subject matter of the present invention is limited to the following examples only. All techniques implemented based on the above description of the invention are within the scope of the invention.
Drawings
FIG. 1 shows IC inhibiting alpha-glucosidase activity of petroleum ether and n-butanol fraction extracts of Coptis chinensis Franch in the example of the present invention 50 A value;
FIG. 2 shows the inhibition curve of the petroleum ether and n-butanol fraction extracts of each coptis chinensis medicinal material in the example of the present invention for inhibiting the activity of alpha-glucosidase;
FIG. 3 is an ultrafiltration chromatogram of an embodiment of the present invention.
Detailed Description
The technical scheme of the application is further described through specific examples.
Example 1 extraction of extracts from different Coptis species and solvents of different polarity
In this example, four kinds of active ingredients in Coptis plants, coptis chinensis (Coptis chinensis), coptis deltoides (C. Deltaidea), coptis omeiensis (C. Omeiensis) and Coptis yunnanensis (C. Teta), were extracted, and the inhibitory effect of their extracts on the activity of alpha-glucosidase was analyzed.
The chemicals used in this example were as follows:
phosphate buffered saline and alpha-glucosidase (EC: 3.2.1.20) were both purchased from Sigma Aldrich. p-nitrophenyl-beta-D-glucopyranoside (PNPG) and acarbose are available from Tokyo chemical industries, inc. Sodium carbonate is from Shanghai Taitan technologies Co. Chemical standards of coptisine, jateorhizine, magnoloine, epiberberine, granada, palmatine and african tetrandrine are purchased from the adult decumber biotechnology company, and berberine is purchased from the national food and drug administration of China. Chromatographic grade acetonitrile and formic acid were purchased from sammer femto technologies. Analytical grade organic reagents used for the preparation of the extracts were all from Chengdu Corp. Deionized water is produced from the milbo pure water system.
The specific process of extracting the active ingredient in this embodiment is as follows:
(1) Separating the rhizome of 4 kinds of rhizoma Coptidis, washing with clear water, drying in air, oven drying at 60deg.C to constant weight, and grinding into 100 mesh powder with pulverizer. Finally, these powders were collected in plastic bags of the corresponding sample names and kept dry.
(2) Weighing 0.5-2.0g of dry powder of rhizoma Coptidis, and extracting with E-916 rapid extractor. Pumping petroleum ether, ethyl acetate and n-butanol in turn to prepare three extraction sites with different polarities. Firstly, a piece of glass fiber filter paper and a piece of metal gasket are placed at the bottom of a 40mL extraction tank, and after a sample is placed, 40-60g of quartz sand is filled in. During the extraction, the temperature and pressure were set continuously at 90℃and 100bar. The extraction parameters are detailed in Table 1.
Table 1E-916 quick extractor parameter settings
(3) After extraction, the corresponding fractions were collected and the solvent was spin dried using a rotary evaporator.
Four coptis medicinal materials are respectively extracted according to the mode, and extracts of petroleum ether, ethyl acetate and n-butanol parts of the four coptis medicinal materials are obtained. Petroleum ether and ethyl acetate extracts were dissolved in 10% dimethylsulfoxide, respectively, and formulated into a solution having a concentration of 0.2g/mL, and n-butanol extract was dissolved in 50% methanol, and formulated into a solution having a concentration of 0.2 g/mL. Finally, these solutions were passed through a filter membrane with a pore size of 0.45 μm and used as sample solutions for subsequent testing.
Example 2 hypoglycemic Activity experiments of extracts of different Coptis species and solvents of different polarity
An experiment of inhibiting α -glucosidase was performed with respect to the hypoglycemic effect of each sample obtained in example 1. The specific process of the experiment is as follows:
(1) The phosphate buffer sheets were taken and placed one by one in 200mL of water to prepare a phosphate buffer solution having a pH of 7.4 and a concentration of 10 mmol/L. Adding 20 mu L of sample solution, 20 mu L of alpha-glycosidase solution with the concentration of 0.3U/mL and 100 mu L of phosphate buffer solution into a 96-well plate, placing the mixture into a 37 ℃ incubator for incubation for 15 minutes, and taking out the mixture;
(2) Adding 20 mu L of PNPG solution with the concentration of 10mmol/L, incubating for 15 minutes again, and taking out;
(3) The reaction was terminated by adding 80. Mu.L of a sodium carbonate solution at a concentration of 0.2mol/L, and the absorbance was measured at 405nm in a SpectraMax iD3 microplate reader.
The calculation formula of the inhibition ratio (%) is as follows: inhibition (%) = [1- (a) SG -A BAG )/(A CG -A BLG )]X 100% (wherein A SG Absorbance for addition of sample solution and alpha-glucosidase; a is that BAG Absorbance of a sample solution to which no α -glucosidase was added; a is that CG Absorbance for alpha-glucosidase without addition of sample solution; a is that BLG Absorbance without addition of neither sample solution nor alpha-glucosidase. The missing solution was replaced with phosphate buffer). All assays were repeated three times. Origin 9 was used.Software 1 calculation of half inhibitory concentration (IC 50 ) And a suppression curve is plotted.
semi-Inhibitory Concentration (IC) of each sample 50 ) As shown in fig. 1, the inhibition curve is shown in fig. 2. The ethyl acetate moiety is not shown because it has no inhibitory activity. By comparing the IC of the other two parts 50 IC of four coptis extracts at petroleum ether part was found 50 The value is about 2-7 times of the n-butanol fraction. Therefore, it was confirmed that the n-butanol fraction had the strongest inhibitory effect on α -glucosidase and was used as a high-activity extraction site.
Acarbose is an effective alpha-glucosidase inhibitor, IC, in the prior art 50 The value was 4.24.+ -. 0.04mg/mL (see FIG. 2). Although acarbose showed better inhibitory activity than each n-butanol extract of coptis chinensis, the maximum inhibition rate was less than 90%, while each of four n-butanol extracts of coptis chinensis was higher than 95%. It can be seen that it is interesting to find potential α -glucosidase inhibitors from the n-butanol fraction of coptis deltoidea. The extract of n-butanol fraction of Coptis deltoidea is also valuable for use in combination with other active substances to make hypoglycemic pharmaceutical compositions.
Further observing n-butanol extract with best hypoglycemic activity of four kinds of rhizoma Coptidis, finding out the highest inhibitory activity of n-butanol extract of Coptis deltoidea on alpha-glucosidase from the inhibition rate curve of four kinds of rhizoma Coptidis, and then, rhizoma Coptidis, coptis yunnanensis and Coptis anthropi, their IC 50 The values were 24.95.+ -. 2.14, 28.24.+ -. 0.12, 28.64.+ -. 0.21 and 29.11.+ -. 0.02mg/mL, respectively.
Thus, the extracts of the n-butanol parts of the coptis chinensis, which are prepared in example 1, have the highest inhibitory activity among the extracts of the petroleum ether, ethyl acetate and n-butanol parts of the four coptis chinensis medicinal materials.
Example 3 identification of sugar-lowering active ingredient and its content in Coptis deltoidea
To further identify the active ingredients inhibiting α -glucosidase in the extract of example 1, an ultrafiltration rapid screening experiment was performed below. The specific process of the experiment is as follows:
(1) Preparing ultrafiltration sample liquid: diluting the sample solution prepared from the n-butanol part extract of the coptis deltoidea to 6mg/mL, and mixing the sample solution for standby.
(2) 100. Mu.L of 1U/mL of alpha-glucosidase was mixed with an equal volume of the mixed sample solution as an experimental group. After incubation at 37℃for 30min, transfer to a 30kDa ultrafiltration tube and centrifuge at 10000g for 10min. 200. Mu.L of phosphate buffer (pH=7.4) was then added and centrifuged at 10000g for 10min to wash away unbound compounds, and the procedure was repeated twice. To obtain ligand-enzyme complex.
(3) Then 200. Mu.L of methanol-water (50:50, v/v, pH=3.30) was added to the ligand-enzyme complex, and after standing for 10min, the mixture was centrifuged at 10000g for 10min and repeated 2 times. Finally, the eluent is collected for UPLC-Q-TOF-MS/MS analysis. The enzyme was used as a blank for the reaction with the sample solution instead of the active enzyme. The preparation process of the denaturing enzyme comprises the following steps: and (3) putting 1U/mL of alpha-glucosidase into a dry thermostat at 60 ℃ for 3min to obtain the inactivated enzyme.
The above experiment was repeated three times.
The ultrafiltration chromatogram obtained is shown in FIG. 3, wherein the solid line (experimental group) represents the compounds that bind specifically to the enzyme, and the dotted line is the blank group for monitoring the compounds that bind non-specifically to the enzyme. The potential activity inhibitor is identified by taking the peak area of the experimental group higher than that of the blank control group as a screening standard.
From the figure, 8 peaks show differences and are considered as potential inhibitors, and all the compounds belong to alkaloid compounds, namely magnolol, granadine, jatrorrhizine, epiberberine, african stephanine, coptisine, palmatine and berberine. Previous studies have screened jatrorrhizine, epiberberine, coptisine, palmatine and berberine from coptis chinensis as alpha-glucosidase inhibitors by off-line ultrafiltration. In this study, magnaline, granadine and africane were first screened from coptis deltoidea as potential inhibitors. Wherein, the content of the granadine in the coptis deltoidea is obviously higher than that of other three coptis medicinal materials. That is, in the extract obtained by the method of the present invention, the component species having the hypoglycemic activity in the coptis chinensis deltoid is more and the content of part of the components (granadine) is higher, which further proves that the extract obtained by the extraction method of the present invention has higher hypoglycemic activity.
It can be deduced from the above experiments that the extract of n-butanol part of coptis deltoidea provided in this embodiment is rich in alkaloid compounds and high in content, and thus can be used as a potential α -glucosidase inhibitor. Especially, the content of the granadine in the coptis deltoidea is obviously higher than that of other three coptis medicinal materials, which further enhances the better alpha-glucosidase inhibition effect of the extract of the n-butanol part of the coptis deltoidea.
Claims (6)
1. An extraction method of coptis chinensis extract for reducing blood sugar is characterized by comprising the following steps:
[1] taking dry powder of the coptis deltoidea medicinal material;
[2] sequentially extracting the dry powder in the step (1) by using petroleum ether, ethyl acetate and n-butanol solvent, wherein the extraction process is performed by using a rapid solvent extractor;
[3] collecting the extracting solution of the n-butanol extraction part in the step (2), concentrating to obtain an extract, namely an extract;
the step [2] specifically comprises the following steps: 2-1, sequentially pumping a plurality of solvents with different polarities into a rapid solvent extractor to prepare extraction sites with different polarities, wherein the solvents comprise n-butanol; 2-2, arranging a gasket and glass fiber filter paper at the bottom of an extraction tank of a rapid solvent extractor in sequence, then placing a sample into the extraction tank, and then filling quartz sand; 2-3, starting a solvent extraction instrument to extract; wherein, in the step [2-3], n-butanol is used as an extraction solvent for four times, the 1 st circulation process is heating for 1-2min, maintaining for 0min and releasing for 5min, and the 2 nd circulation process is heating for 1-2min, maintaining for 2-4min and releasing for 5min; the extraction solvent except n-butanol is circulated for three times, wherein each circulation process is heating for 1-2min, maintaining for 2-4min and releasing for 5min; the solvent scouring time is 2min after the extraction of each extraction solvent is completed, and the gas scouring time is 3min;
the content of the granadine in the coptis deltoidea is higher than that of other coptis plants;
the extract has an inhibition rate of alpha-glucosidase activity higher than 95%, and an IC50 value of 24.95 + -
2.14mg/mL。
2. The method for extracting coptis chinensis extract for reducing blood glucose according to claim 1, wherein the dry powder in step [1] is prepared by the following steps: taking rhizome of coptis deltoidea, cleaning, drying in air, putting into an oven at 60 ℃ to be dried to constant weight, and grinding into 100-mesh powder.
3. An extraction method of coptis chinensis extract for reducing blood glucose according to any one of claims 1 to 2, characterized in that: the mass of the dry powder of the coptis deltoidea medicinal material added into the extraction tank in the step [2-2] is 0.5-2.0g, the specification of the extraction tank is 40ml, and the amount of quartz sand added into the extraction tank is 40-60g.
4. An extraction method of coptis chinensis extract for reducing blood glucose according to any one of claims 1 to 2, characterized in that: in the step [2-3], the temperature in the extraction process is 90 ℃ and the pressure is 100bar.
5. An extract prepared by the extraction method according to any one of claims 1 to 4.
6. Use of the extract according to claim 5 for the preparation of a hypoglycemic agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010900167.9A CN114099574B (en) | 2020-08-31 | 2020-08-31 | Extraction method of coptis chinensis extract for reducing blood glucose and extract thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010900167.9A CN114099574B (en) | 2020-08-31 | 2020-08-31 | Extraction method of coptis chinensis extract for reducing blood glucose and extract thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114099574A CN114099574A (en) | 2022-03-01 |
| CN114099574B true CN114099574B (en) | 2023-04-21 |
Family
ID=80360260
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010900167.9A Active CN114099574B (en) | 2020-08-31 | 2020-08-31 | Extraction method of coptis chinensis extract for reducing blood glucose and extract thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114099574B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115671203B (en) * | 2022-11-24 | 2023-10-27 | 广州中医药大学科技产业园有限公司 | A Chinese medicinal compound extract for lowering blood sugar and blood lipid, and its extraction method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102285982A (en) * | 2011-08-30 | 2011-12-21 | 聊城大学 | Method for separating and purifying monomer compounds from Chinese medicinal herb golden thread |
-
2020
- 2020-08-31 CN CN202010900167.9A patent/CN114099574B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102285982A (en) * | 2011-08-30 | 2011-12-21 | 聊城大学 | Method for separating and purifying monomer compounds from Chinese medicinal herb golden thread |
Non-Patent Citations (2)
| Title |
|---|
| 黄连干预糖尿病并发抑郁症的有效部位筛选;王凌等;《中国临床药理学与治疗学》;20160331;第21卷(第03期);276-278 * |
| 黄连生物碱的提取纯化及降脂作用;侯宏等;《长春中医药大学学报》;20110210;第27卷(第01期);7-8 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114099574A (en) | 2022-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101433565B (en) | Total alkaloid extract of seeds of harmel genus, and preparation thereof | |
| CN105111255A (en) | Extraction and separation method for echinacoside and verbascoside in cistanche | |
| CN102579612A (en) | Method for extracting total alkaloid of aconitum soongaricum | |
| WO2017016176A1 (en) | Cacumen biotae orientalis polyphenol for decreasing uric acid and preparation method and use thereof | |
| CN103776926A (en) | Establishment of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of rabdosia lophanthide medicinal materials and fingerprint spectrum of of rabdosia lophanthide medicinal materials | |
| CN114099574B (en) | Extraction method of coptis chinensis extract for reducing blood glucose and extract thereof | |
| JP3386796B2 (en) | Quality determination method for plants of the genus Salicaceae and / or extracts thereof | |
| CN101735030A (en) | Method for preparing 6-shogaol | |
| CN109374789A (en) | The construction method and detection method of Cortex Phellodendri medicinal material HPLC characteristic spectrum | |
| CN101337060B (en) | Quality control method of Gongyankang grannule | |
| CN105943621A (en) | Preparation method and quality control method of mulberry leaf extract | |
| CN101632722B (en) | Wild buckwheat rhizome polyphenol extract and preparation method thereof | |
| CN106324177B (en) | The discrimination method of ginger in a kind of Chinese medicine compound prescription | |
| CN108152431A (en) | A kind of construction method and quality determining method of rhodiola root broken wall medicine materical crude slice HPLC finger-prints | |
| CN111184763A (en) | Swertia yunnanensis extract and application thereof | |
| CN107050010A (en) | Application of the Cichoric acid in the medicine of anti respiratory syncytial virus is prepared | |
| CN104569190A (en) | Method for rapidly determining four tannin substances in sapium sebiferum leaves | |
| CN114224951A (en) | Industrial preparation method of total alkaloids in coptis and evodia composition | |
| CN113466385A (en) | Selaginella pulvinata total flavone extraction preparation and HPLC fingerprint detection method | |
| CN103055191A (en) | Preparation method and quality detection method of traditional Chinese medicine for treating hematuresis caused by nephritis | |
| CN113209155A (en) | Total alkaloid extract of lysimachia christinae hance and application thereof | |
| CN109575010B (en) | Method for extracting methyl berberine from coptis chinensis extraction mother liquor, high-purity methyl berberine prepared by method and application of high-purity methyl berberine | |
| CN114432415A (en) | Preparation process and quality control method of Inula and Haematitum decoction formula granules | |
| CN102706999B (en) | The analyzing detecting method of corydalis tuber water soluble non-alkaloid compounds | |
| CN114558059B (en) | Radix Sophorae Flavescentis-Coptidis rhizoma extract with antitumor activity, and quality detection method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |