CN114099048B

CN114099048B - A tail vein injection device for male mouse

Info

Publication number: CN114099048B
Application number: CN202111277429.1A
Authority: CN
Inventors: 李帆; 朱艳艳; 胡浩; 胡慧娟; 孙晓明
Original assignee: Xian Jiaotong University
Current assignee: Xian Jiaotong University
Priority date: 2021-10-29
Filing date: 2021-10-29
Publication date: 2022-10-25
Anticipated expiration: 2041-10-29
Also published as: CN114099048A

Abstract

The invention discloses a tail vein injection device for a male mouse, which comprises a table base, wherein a mouse body fixing pipe is horizontally arranged on the table base; one end of the mouse body fixing tube is opened; the gate is inserted into the opening end of the mouse body fixing pipe, and a mouse tail through hole is formed in the gate; the mouse tail clamping groove is a cylinder with openings at two ends, the upper end of the mouse tail clamping groove is arranged right opposite to the mouse tail through hole of the gate, and the testicle protection mechanism is arranged in the mouse tail clamping groove; the testicle protection mechanism comprises a first cushion table, the first cushion table is arranged at one end of the mouse tail clamping groove in a sliding mode and is close to the gate; the second pad table is fixed in the middle of the mouse tail clamping groove and is connected with the first pad table through a spring; a testis clamping pocket is arranged at the end part of the first cushion table, and a flexible protection cushion is arranged in the testis clamping pocket; the invention uses the testis clamping pocket and the flexible protection pad to protect the testis of the mouse; the spring is retracted, stress struggle caused by pressing the testis when the mouse tail is stretched is avoided, and the operation success rate of tail vein injection is effectively improved.

Description

A tail vein injection device for male mouse

Technical Field

The invention belongs to the technical field of medical experimental instruments, and particularly relates to a tail vein injection device for a male mouse.

Background

The experimental animal mouse is more widely applied in various fields while the modern medicine is developed at a high speed; in particular, since the physiological changes, the body metabolism, the biochemical indexes, the pathological process under the disease state and the like of the male mice are not influenced by the estrogen changes, the male mice are more suitable for a plurality of experiments compared with the female mice; the tail vein injection operation of the mice is one of the most basic administration modes aiming at the mice, and in numerous medical research projects and experimental teaching contents, the intervention of drugs through tail vein injection is generally required.

The physiological structure of the male mouse is different from that of the female mouse, and the testis at the tail root of the male mouse is protruded, so that the male mouse is easily extruded in the fixing process and is easily damaged; the existing tail vein fixing device is found in use: compared with female mice, the male mice are more stressed, and struggle more severely when stretching the tail, which affects the success rate of operation, and is closely related to the testis of the male mice being pressed.

The current mouse tail intravenous injection device is not strictly distinguished and considers the applicability of male mice and female mice, and further innovation and design are not carried out on the tail parts of the male mice; meanwhile, aiming at the tail of the mouse, no special tail supporting device is arranged; during injection, the tail of the mouse is in a suspended state and is unstable, so that inconvenience is brought to an operator; secondly, for fixing the bodies of mice with different body types, a movable baffle plate is adopted to compress the front space and the rear space, and the upper space and the lower space are compressed through an upper pressing plate and a lower pressing plate, so that the stress of the mice is more easily caused, the body can be twisted, and the tail vein injection is influenced by the compression type fixing mode; in addition, after the injection operation is finished, the existing device mostly adopts a forced pulling mode to take out the mouse, so that the body of the mouse is damaged; finally, the vein blood vessel developing device at the tail part is single or lack, and the device additionally provided with the light source cannot eliminate dazzling and light reflection caused by the light source, so that the visual interference of an operator is great, the blood vessel is difficult to identify, and the operation success rate is low.

Disclosure of Invention

Aiming at the technical problems in the prior art, the invention provides a tail vein injection device for a male mouse, and aims to solve the technical problem that the conventional tail vein injection device for the mouse is easy to press testes of the male mouse, so that the success rate of injection operation is reduced.

In order to achieve the purpose, the invention adopts the technical scheme that:

the invention provides a tail vein injection device for a male mouse, which comprises a table base, a mouse body fixing pipe, a gate, a mouse tail clamping groove and a testicle protection mechanism, wherein the mouse body fixing pipe is horizontally arranged on the table base; one end of the mouse body fixing tube is opened; the gate is inserted into the opening end of the mouse body fixing pipe, and a mouse tail through hole is formed in the gate;

the mouse tail clamping groove is arranged at the opening end of the mouse body fixing pipe and is fixed on the side edge of the platform base; the rat tail clamping groove is a cylinder with two open ends, the upper end of the rat tail clamping groove is opposite to the rat tail through hole of the gate and extends into the rat body fixing tube; the testicle protection mechanism is arranged in the rat tail clamping groove;

the testicle protection mechanism comprises a first cushion table, a second cushion table, a spring and a flexible protection cushion; the first pad table is arranged at one end of the mouse tail clamping groove in a sliding mode and is close to the gate; the second pad table is fixed in the middle of the mouse tail clamping groove and is connected with the first pad table through a spring;

a first mouse tail through hole is formed in the center of the first pad table, a second mouse tail through hole is formed in the center of the second pad table, and the first mouse tail through hole and the second mouse tail through hole are coaxially arranged; a testis clamping pocket is arranged at the end part of the first cushion platform, and is arranged at one side far away from the second cushion platform; a flexible protective pad is arranged in the testicle clamping pocket.

Furthermore, the testicle protection mechanism also comprises an ischium cushion block; the ischial cushion blocks are symmetrically arranged at preset positions on two sides of the first cushion platform; one end of the ischium cushion block is fixed with the side wall of the first cushion table, and the other end of the ischium cushion block extends outwards to the outer side of the rat tail clamping groove along the radial direction of the ischium cushion block.

Further, the mouse body fixing pipe comprises a plurality of fixing pipe bodies; the sizes of the fixed pipe bodies are different, and the fixed pipe bodies are sequentially overlapped from small to large according to the sizes.

Further, the fixed pipe body comprises a first fixed pipe unit and a second fixed pipe unit; the first fixed pipe unit is a hollow cylindrical structure with two open ends, and the second fixed pipe unit is a conical structure with one open end and one closed end; one end of the first fixed pipe unit is connected with the gate, and the other end of the first fixed pipe unit is detachably fixed with the open end of the second fixed pipe unit; the closed end of the second fixing pipe unit is provided with a breathing hole.

Further, the blood vessel developing device is also included; the blood vessel developing mechanism is arranged on the table base and is arranged below the mouse tail clamping groove.

Further, the blood vessel developing mechanism comprises a developing light source and a light source adjusting knob; the developing light source is arranged on the table base and is arranged below the mouse tail clamping groove; the developing light source is connected with the light source adjusting knob; the developing light source is a red light source.

Further, the device also comprises a visual amplifying mechanism; the visual amplification mechanism is arranged on the table base and is close to the opening end of the mouse body fixing tube; the visual amplification mechanism comprises a fixed arm, an adjusting arm and a visual amplifier; one end of the fixed arm is fixed with the table base, and the other end of the fixed arm is hinged with one end of the adjusting arm; the other end of the adjusting arm is connected with the visual amplifier.

Further, the visual amplifier comprises a lens cone, a first amplifying lens, a second amplifying lens and a linear polarization lens; the lens cone is hinged at the end part of the adjusting arm; the first magnifying lens is arranged at one end of the lens cone, the second magnifying lens is arranged at the other end of the lens cone, and the linear polarization lens is arranged at the front end of the second magnifying lens.

Further, a fixed pipe clamping groove is formed in the table base; the mouse body fixing pipe is horizontally arranged in the fixing pipe clamping groove, and the mouse body fixing pipe can be detachably fixed in the fixing pipe clamping groove.

Furthermore, the spring adopts a micro-elastic spring, and the flexible protection pad is a protruding sponge protection pad.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a tail vein injection device for a male mouse, wherein a testicle protection mechanism is arranged in a mouse tail clamping groove to support the tail of the mouse, so that unstable injection operation caused by hanging the tail is avoided; the end part of a first cushion table of the testicle protection mechanism is provided with a testicle clamping pocket, and the testicle clamping pocket and a flexible protection cushion in the testicle clamping pocket are utilized to effectively protect the testicle of the mouse and avoid being pressed; simultaneously, adopt the spring to link to each other between first pad platform and the second pad platform, tensile rat tail process utilizes the spring to contract back, realizes struggling to tensile buffering, avoids causing the stress that the oppression and produce to the testicle when tensile rat tail to struggle, has effectively improved tail vein injection's operation success rate.

Furthermore, through the bilateral symmetry at first pad platform set up ischium cushion, ischium cushion plays limiting displacement, surpasss to predetermine safe range the effect to the tensile effect of rat tail after, utilizes ischium cushion to form the support to the ischium of mouse, avoids first pad platform to retract completely to the rat tail draw-in groove after, causes the testis atress oppression.

Furthermore, the fixed tube bodies with different sizes are sequentially arranged in a nested manner, and the matched fixed tube bodies are selected for fixing according to the body sizes of mice, so that the comfort of the mouse body in the tail vein injection operation process is ensured, and the mouse body of the mouse is prevented from being pressed; meanwhile, the mouse body fixing pipe can be replaced quickly, the operation is convenient, and the application range of the device is widened.

Furthermore, the blood vessel developing mechanism is arranged below the rat tail clamping groove, and the characteristics of good red light wave wavelength and penetrability of a red light source are utilized, so that the rat tail blood vessel can be displayed conveniently; meanwhile, due to the characteristic of high energy density of red light waves, the heating effect can be achieved, so that the tail blood vessels are in a diastolic state to the maximum extent; through setting up light source adjust knob, be convenient for adjust the intensity of development light source, satisfy different user demands.

Furthermore, by arranging a visual amplification mechanism, the visual field can be amplified by using an amplification lens, and an operator is assisted in identifying the blood vessels at the tail of the mouse; meanwhile, the linear polarization lens can eliminate dazzling and reflection caused by a developing light source, so that the image is layered, and the visual field is clearer.

Furthermore, the mouse body fixing tube adopts a hollow cylindrical structure and a conical structure which are detachably spliced, so that the mouse body can be naturally stretched in a smooth cylindrical space; meanwhile, after the injection is finished, the hollow cylindrical structure and the conical structure are separated, and the mouse is taken out, so that the mouse is prevented from being taken out by strong pulling.

According to the tail vein injection device for the male mice, disclosed by the invention, aiming at the physiological structure characteristics of the male mice, the testis of the male mice is protected in multiple levels through the testis protection mechanism arranged in the mouse tail clamping groove, so that the testis is prevented from being pressed; the fixing tube bodies with different sizes are quickly replaced in a telescoping mode, so that non-compression fixing of mice with different body sizes is met; each fixing tube body is formed by splicing a separable cylindrical structure and a conical structure, so that a mouse is taken out in a pulling-preventing mode; the red light source is used as a developing light source, so that the appearance of blood vessels can be increased; meanwhile, the blood vessels can be dilated by heating, the development is clearer, the light source intensity can be adjusted by a light source adjusting knob, and the lamp is multipurpose; the adjusting arm and the vision amplifying mechanism connected with the adjusting arm can enable an operator to observe the tail vein and the needle insertion part of the mouse more easily, and the linear polarizer can eliminate dazzling and light reflection caused by a light source, so that visual field images are layered and are compared more clearly; although the device for injecting the tail vein of the male mouse is designed aiming at the male mouse, the device does not influence the fixation and the use of the female mouse at all, can also be used for injecting the tail vein of the female mouse, and has the advantages of low manufacturing cost, strong universality and convenient popularization.

Drawings

FIG. 1 is a schematic view of the overall structure of a tail vein injection device according to an embodiment;

FIG. 2 is a schematic view of the combined structure of the rat body fixing tubes of different types in the embodiment;

FIG. 3 is a schematic view of a connection structure of a mouse body fixing tube and a fixing tube clamping groove in the embodiment;

FIG. 4 is a schematic view of a connection structure of the gate and the tail slot in the embodiment;

fig. 5 is a top view of a testicle protection mechanism of an embodiment;

fig. 6 is a cross-sectional view of a testicle protection mechanism of an embodiment;

FIG. 7 is a schematic view of a visual amplifying mechanism in the embodiment;

FIG. 8 is a schematic perspective view of the mouse body fixing tube in the embodiment;

fig. 9 is a schematic diagram of an assembling process of the mouse body fixing tube in the embodiment.

The device comprises 1 base, 2 fixed tube clamping grooves, 3 mouse body fixed tubes, 4 gates, 5 mouse tail clamping grooves, 6 testicle protection mechanisms, 7 developing light sources, 8 light source adjusting knobs, 9 fixed walls, 10 adjusting arms and 11 visual amplifiers, wherein the fixed tube clamping grooves are arranged on the bases; a 111 lens barrel, a 112 first magnifying lens, a 113 second magnifying lens, and a 114 linear polarization lens; 31 fixing the pipe body; 311 a first fixed tube unit, 312 a second fixed tube unit, 313 a breathing hole; 61, a first cushion table, 62, a second cushion table, 63 springs, 64 ischial cushion blocks and 65 flexible protection cushions; 611 testis to clamp the bag.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects of the present invention more apparent, the following embodiments further describe the present invention in detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The invention provides a tail vein injection device for a male mouse, which comprises a table base 1, a fixed tube clamping groove 2, a mouse body fixed tube 3, a gate 4, a mouse tail clamping groove 5, a testicle protection mechanism 6, a blood vessel developing mechanism and a vision amplifying mechanism, wherein the table base is provided with a table base; a fixed pipe clamping groove 2 is formed in the table base 1, and the fixed pipe clamping groove 2 is an arc-shaped groove; the mouse body fixing tube 3 is horizontally arranged in the fixing tube clamping groove 2, and the mouse body fixing tube 3 is detachably fixed in the fixing tube clamping groove 2; one end of the mouse body fixing tube 3 is opened, the other end is closed, the closed end is provided with a breathing hole 313, and the mouse body fixing tube 3 is used for fixing the mouse body of the mouse; the gate 34 is inserted into the opening end of the mouse body fixing tube 3, and a mouse tail through hole is formed in the gate 4 and is used for allowing the mouse tail of a male mouse to penetrate through and enter the mouse tail clamping groove 5; the mouse tail clamping groove 5 is arranged at the opening end of the mouse body fixing tube 3 and is fixed on the side edge of the platform base 1; the mouse tail clamping groove 5 is a cylinder with two open ends; the upper end of the mouse tail clamping groove 5 is arranged right opposite to the mouse tail through hole of the gate 4 and extends into the mouse body fixing tube 3; the testicle protection mechanism 6 is arranged in the rat tail clamping groove 5.

The testicle protection mechanism 6 comprises a first cushion table 61, a second cushion table 62, a spring 63, a ischial cushion block 64 and a flexible protection cushion 65, wherein the first cushion table 61 is slidably arranged at one end of the rat tail clamping groove 5 and is close to the gate 4; the second pad table 62 is fixed in the middle of the mouse tail clamping groove 5, and the spring 63 is arranged between the first pad table 61 and the second pad table 62; one end of the spring 63 is connected with the first pad table 61, and the other end is connected with the second pad table 62; a first rat tail through hole is formed in the center of the first pad table 61, and a second rat tail through hole is formed in the center of the second pad table 62; the first rat tail through hole and the second rat tail through hole are coaxially arranged and are used for rat tails of male mice to penetrate through; the first rat tail through hole and the second rat tail through hole have the same aperture and are both larger than the rat tail diameter of a mouse; preferably, the spring 63 is a micro-spring.

The ischial cushion blocks 64 are symmetrically arranged at preset positions on two sides of the first cushion table 61; one end of the ischial cushion block 64 is fixed with the side wall of the first cushion table 61, and the other end extends outwards along the radial direction of the ischial cushion block 64 to the outer side of the rat tail clamping groove 5; a testis clamping pocket 611 is arranged at the end part of the first cushion table 61, the testis clamping pocket 611 is arranged at one side far away from the second cushion table 62, and a flexible protection cushion 65 is arranged in the testis clamping pocket 611; preferably, the flexible protection pad 65 is a protruding sponge protection pad.

The mouse body fixing pipe 3 comprises a plurality of fixing pipe bodies 31, the shape and the structure of the fixing pipe bodies 31 are similar, but the sizes of the fixing pipe bodies are different, and the fixing pipe bodies are sequentially overlapped from small to large according to the sizes; the fixed pipe body 31 includes a first fixed pipe unit 311 and a second fixed pipe unit 312; the first fixed pipe unit 311 is a hollow cylindrical structure with two open ends, and the second fixed pipe unit 312 is a conical structure with one open end and one closed end; one end of the first fixed pipe unit 311 is connected to the shutter 4, and the other end is detachably fixed to the open end of the second fixed pipe unit 312; the closed end of the second fixed pipe unit 312 is provided with a breathing hole 313.

The blood vessel developing mechanism is arranged on the table base 1 and is arranged below the mouse tail clamping groove 5; the blood vessel developing mechanism comprises a developing light source 7 and a light source adjusting knob 8; the developing light source 7 is arranged on the table base 1 and is arranged below the mouse tail clamping groove 5; the developing light source 7 is connected with a light source adjusting knob 8; the developing light source 7 is a red light source; preferably, the developing light source 7 is a 8V-50W red spotlight.

The visual amplification mechanism is arranged on the table base 1 and is close to the opening end of the mouse body fixing tube 3; the vision amplifying mechanism comprises a fixed arm 9, an adjusting arm 10 and a vision amplifier 11; one end of a fixed arm 9 is fixed with the table base 1, and the other end of the fixed arm 9 is hinged with one end of an adjusting arm 10; the other end of the adjusting arm 10 is connected with a visual amplifier 11; the visual amplifier 11 includes a lens barrel 111, a first magnifying lens 112, a second magnifying lens 113, and a linearly polarizing lens 114; the lens barrel 111 is hinged at the end of the adjusting arm 10; a first magnification lens 112 is disposed at one end of the lens barrel 111, a second magnification lens 113 is disposed at the other end of the lens barrel 111, and a linearly polarizing lens 114 is disposed at the front end of the second magnification lens 113.

The working principle and the using method are as follows:

aiming at the physiological structure characteristics of a male mouse, the testicle protection mechanism is arranged in the mouse tail clamping groove and has triple protection measures; firstly, the testicle clamping pocket is arranged at the end part of the first cushion table, so that after a mouse is fixed, a testicle is positioned in the inwards concave testicle clamping pocket, and the flexible protection cushion is arranged in the testicle clamping pocket, so that the testicle can be effectively protected from being pressed; secondly, a spring is arranged between the first pad table and the second pad table, and when the mouse tail is stretched, the stretching force of the mouse tail is buffered by utilizing the retraction of the spring so as to protect the testes; third, set up ischium cushion through the bilateral symmetry at first pad platform, when exceeding predetermined safe range to the tensile force of rat tail, first cushion retracts back completely to the rat tail draw-in groove in, and at this moment, ischium cushion can tightly push up with the ischium of mouse, prevents the further atress of testicle.

According to the invention, the testicle protection mechanism is arranged in the rat tail clamping groove, so that the tail of the mouse can be effectively supported, and the problem of unstable success rate of tail vein injection operation caused by hanging of the rat tail is avoided; the mouse body fixing pipe is arranged by matching a cylindrical structure with a conical structure, so that compression type fixation of the mouse body is avoided, and the mouse body can be naturally unfolded in a smooth cylindrical space.

The fixing pipe bodies with different sizes are sequentially arranged in a telescopic mode, so that the fixing pipe bodies can be quickly replaced according to use requirements, mice with different body sizes can be fixed, and the mice are prevented from being pressed; the fixed pipe body adopts two fixed pipe units to dismantle and joins in marriage and set up, and the injection is accomplished the back, takes out the mouse smoothly with two fixed pipe unit splits, avoids dragging the brute force of mouse.

Aiming at the problem of a tail vein blood vessel developing device, a developing light source is arranged below a tail clamping groove, and a vision amplifying mechanism is arranged above a table base; the developing light source is a red spotlight with 8V-50W, and the characteristics of longer red light wavelength and good penetrability are utilized; in particular in biological tissues, it is easier to visualize blood-filled vessels; meanwhile, the red wave light wave energy density is high, and the heating function can be achieved; the tail blood vessel can be in a diastolic state to the maximum extent by heating with a 50W red spotlight, and the intensity of the developing light source can be adjusted by a light source adjusting knob according to needs; the magnifying lens in the vision magnifying mechanism can magnify the visual field, assists an operator to identify blood vessels, and the linear polarizer can eliminate dazzling and reflection caused by a light source below, so that the image is layered, and the visual field is clear.

Examples

As shown in fig. 1-9, the present embodiment provides a tail vein injection device for male mice, which includes a table base 1, a fixing tube slot 2, a mouse body fixing tube 3, a gate 4, a rat tail slot 5, a testicle protection mechanism 6, a blood vessel developing mechanism, and a visual amplification mechanism.

The table base 1 is symmetrically provided with fixed pipe clamping grooves 2, and the fixed pipe clamping grooves 2 are arc-shaped grooves; the arc-shaped groove is formed in the surface of the table base 1, and a mouse body fixing tube 3 is arranged in each fixing tube clamping groove 2; the mouse body fixing tube 3 is horizontally arranged in the fixing tube clamping groove 2, and the mouse body fixing tube 3 is detachably fixed in the fixing tube clamping groove 2; one end of the mouse body fixing tube 3 is opened, the other end is closed, a breathing hole 313 is formed in the closed end, and the mouse body fixing tube 3 is used for fixing a mouse body of a mouse.

The open end of each mouse body fixing pipe 3 is provided with a gate 4, and the gate 4 is inserted into the open end of the mouse body fixing pipe 3; a rat tail through hole is formed in the gate 4 and is used for allowing a rat tail of a male mouse to penetrate through and enter the rat tail clamping groove 5; the mouse tail clamping groove 5 is formed in the opening end of the mouse body fixing tube 3, and the mouse tail clamping groove 5 is vertically fixed to the side edge of the table base 1; the rat tail clamping groove 5 is a cylinder with two open ends, the upper end of the rat tail clamping groove 5 is arranged opposite to the rat tail through hole of the gate 4 and extends into the rat body fixing tube 3; the testicle protection mechanism 6 is arranged in the rat tail clamping groove 5.

The testicle protection mechanism 6 comprises a first cushion table 61, a second cushion table 62, a spring 63, a ischial cushion block 64 and a flexible protection cushion 65, wherein the first cushion table 61 is slidably arranged at one end of the rat tail clamping groove 5 and is close to the gate 4; the second pad table 62 is fixed in the middle of the mouse tail clamping groove 5, and the spring 63 is arranged between the first pad table 61 and the second pad table 62; the spring 63 is a micro-elastic spring, one end of the spring 63 is connected with the first cushion table 61, and the other end of the spring 63 is connected with the second cushion table 62; a first mouse tail through hole is formed in the center of the first pad table 61, and a second mouse tail through hole is formed in the center of the second pad table 62; the first rat tail through hole and the second rat tail through hole are coaxially arranged and are used for rat tails of male mice to penetrate through; the first rat tail through hole and the second rat tail through hole have the same aperture and are both larger than the rat tail diameter of a mouse.

The ischial cushion blocks 64 are symmetrically arranged at preset positions on two sides of the first cushion table 61; one end of the ischial cushion block 64 is fixed with the side wall of the first cushion table 61, and the other end extends outwards along the radial direction of the ischial cushion block 64 to the outer side of the rat tail clamping groove 5; a testis clamping pocket 611 is arranged at the end part of the first cushion table 61, the testis clamping pocket 611 is arranged at one side far away from the second cushion table 62, and a flexible protection cushion 65 is arranged in the testis clamping pocket 611; wherein the flexible protection pad 65 is a raised sponge protection pad; in the embodiment, the exposed mouse tail is supported through the mouse tail clamping groove and the embedded testicle protection mechanism; the testis clamping pocket and the sponge protection pad protruding from the testis clamping pocket are used for coating the testis of the male mouse, so that the testis is prevented from being extruded and damaged; meanwhile, the first cushion table and the second cushion table are drawn by the micro-elastic springs, and the testis is prevented from being subjected to overlarge extrusion force by utilizing the retraction buffer effect of the micro-elastic springs.

The mouse body fixing pipe 3 comprises three fixing pipe bodies 31, the three fixing pipe bodies 31 are similar in appearance structure, different in size and sequentially overlapped from small to large in size; in the embodiment, the first fixing tube body is 3.6cm in diameter and 10cm in length, and is suitable for mice with the weight of more than 30 g; the diameter of the second fixed tube body is 3.2cm, the length of the second fixed tube body is 8cm, and the second fixed tube body is suitable for mice with the weight ranging from 20g to 30 g; the diameter of the third fixing tube body is 2.8cm, the length of the third fixing tube body is 6cm, and the third fixing tube body is suitable for a lower mouse with the weight of below 20 g; the fixed pipe body with three kinds of specifications overlaps the overlapping combination in proper order and is in the same place, and quick replacement according to the use needs had both satisfied the mouse of different sizes of bodily form and has fixed, also can not cause the oppression to the mouse to realize the mouse of the fixed different sizes of mode of non-oppression formula.

The fixed pipe body 31 includes a first fixed pipe unit 311 and a second fixed pipe unit 312; the first fixed pipe unit 311 is a hollow cylindrical structure with two open ends, and the second fixed pipe unit 312 is a conical structure with one open end and one closed end; one end of the first fixed pipe unit 311 is connected to the shutter 4, and the other end is detachably fixed to an open end of the second fixed pipe unit 312; the closed end of the second fixing tube unit 312 is provided with a breathing hole 313; after the injection is completed, the first fixed pipe unit 311 is separated from the second fixed pipe unit 312, so that the mouse can be taken out of the fixed pipe body 31, and the mouse can be prevented from being taken out by strong pulling.

The blood vessel developing mechanism is arranged on the table base 1 and is arranged below the mouse tail clamping groove 5; the blood vessel developing mechanism comprises two developing light sources 7 and a light source adjusting knob 8; the two developing light sources 7 are arranged on the table base 1 and are respectively arranged below the two tail clamping grooves 5; the developing light source 7 is connected with a light source adjusting knob 8 through a lead; the developing light source 7 is a red light source; the developing light source 7 adopts a red spotlight of 8V-50W; the mouse tail vein can be displayed more clearly by the developing light source, an operator can conveniently identify the blood vessel, and the blood vessel can be dilated to the maximum extent by heating the developing light source; the light source adjusting knob can adjust the intensity of the light source according to the requirements of an operator.

The visual amplification mechanism is arranged on the table base 1 and is close to the opening end of the mouse body fixing tube 3; the vision amplifying mechanism comprises a fixed arm 9, two adjusting arms 10 and two vision amplifiers 11; one end of a fixed arm 9 is fixed with the table base 1, and two adjusting arms 10 are respectively arranged at two sides of the fixed arm 9; the other end of the fixed arm 9 is hinged with one end of the adjusting arm 10; the other end of the adjusting arm 10 is connected with a visual amplifier 11; the adjusting arm 10 includes a first adjusting rod, a second adjusting rod and a third adjusting rod, one end of the first adjusting rod is hinged to the fixing arm 9, the other end of the first adjusting rod is sequentially hinged to the second adjusting rod and the third adjusting rod, and the third adjusting rod is hinged to the visual amplifier 11.

The visual amplifier 11 includes a lens barrel 111, a first magnifying lens 112, a second magnifying lens 113, and a linearly polarizing lens 114; the lens barrel 111 is hinged at the end of the adjusting arm 10; a first magnifying lens 112 is disposed at one end of the lens barrel 111, a second magnifying lens 113 is disposed at the other end of the lens barrel 111, and a linearly polarizing lens 114 is disposed at the front end of the second magnifying lens 113; two magnifying lenses in the vision magnifying mechanism can help an operator to better position an injection site, hold the injection direction and the puncture strength, and the linear polarizing lens can eliminate dazzling and reflection caused by a light source, so that visual field images are layered and are more clear in contrast.

In order to solve the problem of testis compression of a male mouse, the tail vein injection device for the male mouse is characterized in that a mouse tail clamping groove is formed in the opening end of a mouse body fixing tube, and a testis protection mechanism is arranged in the mouse tail clamping groove; after a male mouse is fixed, the testicle is positioned in the inward-concave testicle clamping pocket, and the protruding sponge protection pad in the testicle clamping pocket can protect the testicle from being extruded; when an operator stretches a rat tail, a first cushion table in the testicle protection mechanism can retract through a micro-elastic spring to buffer the tension force and protect the testicles; if the tension of the stretched rat tail exceeds a safety range, the first pad table is completely retracted into the rat tail clamping groove, at the moment, the ischial cushion blocks can prop against the ischial bones of the mouse, an operator is prompted to stop stretching the tail of the mouse, and the testes are prevented from being further stressed; in the invention, in order to ensure that the device can be suitable for mice with different sizes, increase the universality of the device and ensure that the experimental mouse is naturally and smoothly fixed, the mouse body fixing pipe adopts the way that fixing pipe bodies with different sizes are sequentially nested according to the size; specifically, a fixed tube body with a small specification can be embedded into a fixed tube body with a larger size, a fixed tube body with a smaller specification can be embedded into a fixed tube body with a small specification, and an operator can quickly replace the fixed tube body as required to fix the mouse body of the mouse; after the injection operation is finished, an operator only needs to take out the fixed tube body and separate the two fixed tube units in the fixed tube body, so that the mouse can be smoothly taken out, and the way of taking out the mouse by strong pulling is avoided; in order to increase the identification and locking of the tail vein, a developing light source is arranged below the tail clamping groove, the developing light source can increase the development of the blood vessel and can also heat the tail part to enable the tail vein to relax to the maximum degree, and the tail vein dilator has two functions; the light source intensity of the developing light source is adjusted by the light source adjusting knob; in order to make an operator more easily observe the tail vein of the mouse, the adjusting arm is connected with a vision amplifying mechanism which can locally amplify the injection part; in order to eliminate dazzling and reflection caused by a light source below, a linear polarization lens is arranged below the vision amplifying mechanism, and the optimal visual field can be found by rotating the polarization lens to filter useless light, so that the image is layered and the contrast is clearer.

The above-described embodiment is only one of the embodiments that can implement the technical solution of the present invention, and the scope of the present invention to be claimed is not limited to the embodiment, but includes any changes, substitutions and other embodiments that can be easily conceived by those skilled in the art within the technical scope of the present invention disclosed.

Claims

1. A tail intravenous injection fixing device for a male mouse is characterized by comprising a table base (1), a mouse body fixing tube (3), a gate (4), a mouse tail clamping groove (5) and a testis protection mechanism (6), wherein the mouse body fixing tube (3) is horizontally arranged on the table base (1); one end of the mouse body fixing tube (3) is opened; the gate (4) is inserted into the opening end of the mouse body fixing pipe (3), and a mouse tail through hole is formed in the gate (4);

the mouse tail clamping groove (5) is arranged at the opening end of the mouse body fixing pipe (3) and is fixed on the side edge of the platform base (1); the mouse tail clamping groove (5) is a cylinder with openings at two ends, the upper end of the mouse tail clamping groove (5) is opposite to the mouse tail through hole of the gate (4) and extends into the mouse body fixing tube (3); the testicle protection mechanism (6) is arranged in the rat tail clamping groove (5);

the testicle protection mechanism (6) comprises a first cushion table (61), a second cushion table (62), a spring (63) and a flexible protection cushion (65); the first pad table (61) is arranged at one end of the tail clamping groove (5) in a sliding mode and is close to the gate (4); the second pad table (62) is fixed in the middle of the mouse tail clamping groove (5), and the second pad table (62) is connected with the first pad table (61) through a spring (63);

a first rat tail through hole is formed in the center of the first pad table (61), a second rat tail through hole is formed in the center of the second pad table (62), and the first rat tail through hole and the second rat tail through hole are coaxially arranged; a testis clamping pocket (611) is arranged at the end part of the first cushion table (61), and the testis clamping pocket (611) is arranged at one side far away from the second cushion table (62); a flexible protective pad (65) is arranged in the testicle clamping pocket (611).

2. A tail vein injection fixation device for male mice according to claim 1, characterized in that the testicle protection means (6) further comprises an ischial pad (64); the ischial cushion blocks (64) are symmetrically arranged at preset positions on two sides of the first cushion table (61); one end of the ischial cushion block (64) is fixed with the side wall of the first cushion table (61), and the other end of the ischial cushion block (64) extends outwards along the radial direction of the ischial cushion block to the outer side of the rat tail clamping groove (5).

3. A tail vein injection fixation device for male mice according to claim 1, characterized in that the mouse body fixation tube (3) comprises several fixation tube bodies (31); the fixed pipe bodies (31) are different in size and are sequentially overlapped from small to large according to the size.

4. A tail vein injection fixing device for male mice according to claim 3, characterized in that the fixing tube body (31) comprises a first fixing tube unit (311) and a second fixing tube unit (312); the first fixed pipe unit (311) is a hollow cylindrical structure with two open ends, and the second fixed pipe unit (312) is a conical structure with one open end and one closed end; one end of the first fixed pipe unit (311) is connected with the gate (4), and the other end of the first fixed pipe unit is detachably fixed with the open end of the second fixed pipe unit (312); the closed end of the second fixed pipe unit (312) is provided with a breathing hole (313).

5. The caudal vein injection fixation device for male mice according to claim 1, further comprising a vascular visualization mechanism; the blood vessel developing mechanism is arranged on the table base (1) and is arranged below the mouse tail clamping groove (5).

6. A caudal vein injection fixation device for male mice according to claim 5, wherein the blood vessel visualization mechanism comprises a visualization light source (7) and a light source adjustment knob (8); the developing light source (7) is arranged on the table base (1) and is arranged below the mouse tail clamping groove (5); the developing light source (7) is connected with the light source adjusting knob (8); the developing light source (7) is a red light source.

7. The caudal intravenous fixation device for a male mouse of claim 1, further comprising a visual magnification mechanism; the vision amplification mechanism is arranged on the table base (1) and is close to the opening end of the mouse body fixing tube (3); the visual amplification mechanism comprises a fixed arm (9), an adjusting arm (10) and a visual amplifier (11); one end of a fixed arm (9) is fixed with the table base (1), and the other end of the fixed arm (9) is hinged with one end of an adjusting arm (10); the other end of the adjusting arm (10) is connected with a visual amplifier (11).

8. The caudal vein injection fixation device for male mice according to claim 7, wherein the visual magnifier (11) comprises a lens barrel (111), a first magnifying lens (112), a second magnifying lens (113), and a linearly polarizing lens (114); the lens barrel (111) is hinged at the end part of the adjusting arm (10); a first magnifying lens (112) is disposed at one end of a lens barrel (111), a second magnifying lens (113) is disposed at the other end of the lens barrel (111), and a linearly polarizing lens (114) is disposed at the front end of the second magnifying lens (113).

9. The tail vein injection fixing device for the male mice as claimed in claim 1, wherein the table base (1) is provided with a fixing tube clamping groove (2); the mouse body fixing pipe (3) is horizontally arranged in the fixing pipe clamping groove (2), and the mouse body fixing pipe (3) is detachably fixed in the fixing pipe clamping groove (2).

10. A caudal intravenous fixation device for male mice as claimed in claim 1, wherein the spring (63) is a micro-spring and the flexible protection pad (65) is a protruding sponge protection pad.