CN114097350A - Artificial accelerated aging method for pelleted seeds - Google Patents
Artificial accelerated aging method for pelleted seeds Download PDFInfo
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- CN114097350A CN114097350A CN202111407568.1A CN202111407568A CN114097350A CN 114097350 A CN114097350 A CN 114097350A CN 202111407568 A CN202111407568 A CN 202111407568A CN 114097350 A CN114097350 A CN 114097350A
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- aging
- seeds
- pelleted seeds
- soaked
- pelleted
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses an artificial accelerated aging method for pelleted seeds, which uses fine sand and water to be uniformly mixed as an aging matrix, and does not cause cracking of the pelleted seeds while uniformly supplying water; the method provided by the invention combines the advantages of chemical aging and high-temperature high-humidity aging methods, the filter paper below the pelleted seeds is soaked by 50% ethanol, the defects that the pelleted seeds are easy to mildew due to microorganism infection are effectively overcome, meanwhile, the chemical aging is carried out by using the ethanol, the high-temperature high-humidity aging time is shortened, and the pelleted seeds do not need to be soaked in an organic solvent like the prior art, so that the unnatural degradation of the pelleted seeds is caused.
Description
Technical Field
The invention belongs to the technical field of seed culture, and particularly relates to an artificial accelerated aging method for pelleted seeds.
Background
The artificial accelerated aging method is a method for detecting the quality of seeds, and the accelerated aging measurement can predict the storage resistance and the growth performance of the seeds. The artificial accelerated aging measuring method is a common method for measuring the seed activity, and has good reliability and small application limitation.
One of the existing seed aging methods is to put seeds in a high-temperature and high-humidity environment in a short time under artificial control, so that the seeds absorb water from the humid environment, the water content of the seeds is improved, and the seeds are rapidly aged by utilizing high temperature. The seed vigor is determined by comparing the germination rate. However, this method has disadvantages that the time required for the aging treatment is long, and the seeds are easily infected with microorganisms to cause mold. Other common seed aging methods are to soak seeds in methanol or ethanol for several minutes or dozens of minutes, then take out the seeds and dry the seeds, and then measure the germination rate of the seeds, but the method has the disadvantages of too short treatment time, inconvenient operation, easy influence of the concentration of organic solvents and the operation of workers on the germination rate of the seeds, and the existing researches find that the seed aging caused by a simple chemical aging method is different from the naturally aged seeds in the ultrastructure, so that the chemical aging result cannot completely reflect the seed aging result under the preservation condition.
In recent years, more and more seeds have been subjected to pelleting processes, especially for small and particulate seeds. The pelleted seed is a pelleted seed formed after being coated and processed by a seed coating agent, the shape of the pelleted seed is changed into a sphere or an ellipsoid through pelleting, and the weight of the pelleted seed is 1-60 times of that of a naked seed before pelleting. The method is suitable for the naked seeds which are not pelletized, and the pelletized seeds are aged by the method, so that the pelletized seeds are cracked and broken, the state of the pelletized seeds is changed, and the subsequent research is not facilitated; on the other hand, pelleted seeds absorb water more slowly from a humid environment and require more time for aging treatment.
Therefore, it is very critical to develop an artificial accelerated aging method of pelleted seeds that can solve the above problems.
Disclosure of Invention
The invention aims to provide an artificial accelerated aging method for pelleted seeds.
The object of the invention is achieved by the following steps:
step 1: mixing fine sand with water, spreading on the bottom of a culture dish, and covering a layer of filter paper soaked by water or 50% ethanol on the fine sand;
step 2: placing the pelleted seeds on soaked filter paper, sealing the culture dish with a sealing film, placing the culture dish in a constant temperature and humidity box, aging for 10 days, sampling and detecting every day, and keeping the filter paper soaked by water or 50% ethanol during aging.
Compared with the prior art, the invention has the following technical effects:
the method provided by the invention combines the advantages of chemical aging and high-temperature high-humidity aging methods, and the filter paper below the seeds is soaked by 50% ethanol, so that the defects that the seeds are easily infected by microorganisms to mildew and the like can be prevented; meanwhile, the chemical aging is carried out by using ethanol, so that the time of high-temperature high-humidity aging is shortened, and the seeds do not need to be soaked in an organic solvent like the prior art, thereby causing unnatural deterioration of the seeds. In addition, the fine sand is uniformly mixed with water to be used as an aging matrix, so that the cracking of the pelleted seeds can not be caused while the water is uniformly supplied.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to be limiting in any way, and any modifications or alterations based on the teachings of the present invention are intended to fall within the scope of the present invention. The invention comprises the following steps:
step 1: mixing fine sand with water, spreading on the bottom of a culture dish, and covering a layer of filter paper soaked by water or 50% ethanol on the fine sand;
step 2: placing the pelleted seeds on soaked filter paper, sealing the culture dish with a sealing film, placing the culture dish in a constant temperature and humidity box, aging for 10 days, sampling and detecting every day, and keeping the filter paper soaked by water or 50% ethanol during aging.
Preferably, the conditions of the constant temperature and humidity chamber are as follows: 45 ℃ and RH 100%.
Preferably, the filter paper is soaked with 50% ethanol.
Preferably, the upper surface of the bottom of the petri dish is horizontal.
Example 1:
step 1: mixing fine sand with water, spreading on the bottom of a culture dish, and covering a layer of filter paper soaked by water on the fine sand;
step 2: putting MS yunyan 87 pelleted seeds on soaked filter paper, wherein the seeds are not stacked and overlapped, sealing a culture dish by using a sealing film and then putting the culture dish in a constant temperature and humidity box, and the conditions of the constant temperature and humidity box are as follows: aging at 45 deg.C and RH 100% for 10 days while keeping the filter paper wet. Sampling is carried out once every day, the germination potential and the germination potential are detected after the seeds are dried back, the average germination time and the germination index are calculated, and specific results are shown in the following table 1. As a result, the seeds in the part after 10 days of aging have a mildew phenomenon, the pelleted seeds only crack a fine line, and the pelleted seeds do not crack after drying. The detection shows that the germination potential is reduced to 0 and the aging is required for 8 days, the germination rate is reduced to 0 and the aging is required for 10 days, and the germination potential, the average germination time and the germination index rule in the aging period are good.
Table 1 example 1 seed germination percentage
| Days of aging | Germination vigor (%) | Percentage of germination (%) | Average germination time | Index of germination |
| ck(0d) | 99.0 | 99.0 | 5.10 | 19.48 |
| 1d | 99.0 | 100.0 | 5.60 | 18.10 |
| 2d | 97.3 | 98.0 | 5.52 | 17.97 |
| 3d | 96.0 | 97.0 | 6.11 | 15.96 |
| 4d | 94.0 | 97.3 | 6.34 | 15.47 |
| 5d | 69.0 | 88.0 | 7.25 | 12.42 |
| 6d | 22.7 | 80.0 | 8.35 | 9.79 |
| 7d | 19.3 | 69.7 | 8.46 | 8.45 |
| 8d | 3.7 | 42.0 | 9.38 | 4.65 |
| 9d | 0 | 8.3 | 11.34 | 0.76 |
| 10d | 0 | 1.0 | -- | 0.09 |
Example 2:
step 1: mixing fine sand with water, spreading on the bottom of a culture dish, and covering a layer of filter paper soaked by 50% ethanol on the fine sand;
step 2: putting MS yunyan 87 pelleted seeds on soaked filter paper, wherein the seeds are not stacked and overlapped, sealing a culture dish by using a sealing film and then putting the culture dish in a constant temperature and humidity box, and the conditions of the constant temperature and humidity box are as follows: the temperature is 45 ℃, the RH is 100 percent, the aging time is 10-15 days, and the filter paper is soaked by 50 percent ethanol every other aging period. Sampling is carried out once a day, the germination potential and the germination potential are detected, the average germination time and the germination index are calculated, and specific results are shown in the following table 2. After aging for 10 days, no mildew is found on the seeds, the pelleted seeds only crack a fine line, and the pelleted seeds do not crack after drying. The detection shows that the germination potential is reduced to 0 and aging is required for 7 days, the germination rate is reduced to 0 and aging is required for 9 days, and the germination potential, the average germination time and the germination index rule in the aging period are good.
Table 2 example 2 seed germination percentage
| Days of aging | Germination vigor (%) | Percentage of germination (%) | Average germination time | Index of germination |
| ck(0d) | 99.0 | 99.0 | 5.10 | 19.48 |
| 1d | 97.7 | 99.3 | 5.59 | 18.07 |
| 2d | 96.7 | 97.0 | 5.86 | 16.65 |
| 3d | 89.3 | 92.7 | 6.44 | 14.54 |
| 4d | 76.0 | 82.7 | 6.76 | 12.50 |
| 5d | 48.3 | 76.3 | 7.60 | 10.19 |
| 6d | 20.7 | 56.7 | 8.14 | 7.13 |
| 7d | 6.7 | 43.0 | 9.16 | 4.81 |
| 8d | 0 | 16.0 | 10.58 | 1.56 |
| 9d | 0 | 1.7 | -- | 0.15 |
| 10d | 0 | 0 | -- | 0.09 |
Claims (4)
1. An artificial accelerated aging method for pelleted seeds is characterized by comprising the following steps:
step 1: mixing fine sand with water, spreading on the bottom of a culture dish, and covering a layer of filter paper soaked by water or 50% ethanol on the fine sand;
step 2: firstly placing the pelleted seeds on soaked filter paper, sealing a culture dish, placing the culture dish in a constant-temperature constant-humidity box, aging for 10-15 days, sampling the germination rate once every other day, and keeping the filter paper soaked by water or 50% ethanol during aging.
2. The method for artificially accelerating the aging of pelleted seeds as claimed in claim 1, wherein the conditions of the constant temperature and humidity chamber are as follows: 45 ℃ and RH 100%.
3. The method for artificially accelerated aging of pelleted seeds of claim 1, wherein the filter paper is soaked with 50% ethanol.
4. The artificially accelerated aging method of pelleted seeds of claim 1, wherein the fine sand spread on the bottom of the petri dish has an upper surface level.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111407568.1A CN114097350A (en) | 2021-11-24 | 2021-11-24 | Artificial accelerated aging method for pelleted seeds |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111407568.1A CN114097350A (en) | 2021-11-24 | 2021-11-24 | Artificial accelerated aging method for pelleted seeds |
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| CN114097350A true CN114097350A (en) | 2022-03-01 |
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| CN202111407568.1A Pending CN114097350A (en) | 2021-11-24 | 2021-11-24 | Artificial accelerated aging method for pelleted seeds |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341816A (en) * | 2008-08-18 | 2009-01-14 | 中国农业大学 | Accelerated ageing measuring method for alfalfa seed |
| US20160236848A1 (en) * | 2013-11-08 | 2016-08-18 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for improving the storability of seeds |
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2021
- 2021-11-24 CN CN202111407568.1A patent/CN114097350A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341816A (en) * | 2008-08-18 | 2009-01-14 | 中国农业大学 | Accelerated ageing measuring method for alfalfa seed |
| US20160236848A1 (en) * | 2013-11-08 | 2016-08-18 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for improving the storability of seeds |
Non-Patent Citations (2)
| Title |
|---|
| 曲永祯编著: "《种子加工技术及设备》", 31 December 2002, 中国农业出版社 * |
| 邓杰等: "老化处理对不同活力玉米种子萌发期及幼苗期生长的影响", 《种子》 * |
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Application publication date: 20220301 |