CN114085414A - Hydrogel-loaded polyimide bioactive material and preparation method and application thereof - Google Patents
Hydrogel-loaded polyimide bioactive material and preparation method and application thereof Download PDFInfo
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
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- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2379/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2361/00 - C08J2377/00
- C08J2379/04—Polycondensates having nitrogen-containing heterocyclic rings in the main chain; Polyhydrazides; Polyamide acids or similar polyimide precursors
- C08J2379/08—Polyimides; Polyester-imides; Polyamide-imides; Polyamide acids or similar polyimide precursors
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- C08J2429/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal, or ketal radical; Hydrolysed polymers of esters of unsaturated alcohols with saturated carboxylic acids; Derivatives of such polymer
- C08J2429/02—Homopolymers or copolymers of unsaturated alcohols
- C08J2429/04—Polyvinyl alcohol; Partially hydrolysed homopolymers or copolymers of esters of unsaturated alcohols with saturated carboxylic acids
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
Abstract
The invention discloses a hydrogel-loaded polyimide bioactive material, and a preparation method and application thereof, and belongs to the technical field of biomaterials. The preparation method comprises the steps of immersing a polyimide substrate into a strong alkaline solution for hydrothermal treatment, coating hydrogel on the surface of the treated polyimide substrate, then immersing the polyimide substrate in a growth factor solution, and sterilizing to obtain the hydrogel-loaded polyimide bioactive material. The hydrogel-loaded polyimide bioactive material provided by the invention is flexible in processing mode, and can be used for preparing base materials with different shapes to match different use environments; the polyvinyl alcohol single butyric acid double-network hydrogel has a simple and controllable gel forming mode and high curing speed, and the prepared polyimide bioactive material loaded with the hydrogel has the elastic modulus and the strength close to human bone tissues, and has good biocompatibility, osteogenesis performance and excellent antibacterial performance.
Description
Technical Field
The invention relates to the technical field of biological materials, in particular to a hydrogel-loaded polyimide bioactive material and a preparation method and application thereof.
Background
The biological material is a novel high-technology material for diagnosing, treating, repairing or replacing human tissues and organs or enhancing the functions of the human tissues and organs, relates to the health of hundreds of millions of people, saves the lives of tens of thousands of critically ill patients, obviously reduces the death rate of serious diseases such as cardiovascular diseases, cancers, wounds and the like, and plays an important role in improving the life quality and the health level of the patients and reducing the medical cost.
Currently, millions of procedures for bone tissue repair fixation, either autologous (patient-own) bone grafting and allogeneic (from others) bone grafting, are performed annually using biologically inert metallic materials. However, metal fixation devices often require a second surgery to remove, which is likely to cause bacterial infection. Autologous bone grafting can cause additional morbidity in the donor site and the risk of disease transmission by allogeneic bone grafting is unpredictable. In recent years, bone tissue engineering scaffold materials have been substantially developed which promote bone regeneration and repair at bone defect sites without the above-mentioned risks.
Hydrogels are water-swollen cross-linked polymer molecular networks, generally formed by polymerization of monomers or by hydrogen bonding or van der waals forces between molecular chains. In the past decades, interest has been paid due to its particular application in the field of biomaterials. The medical grade polyvinyl alcohol is different from chemical grade polyvinyl alcohol, is an extremely safe high molecular organic matter, has no toxicity or side effect on a human body, has good biocompatibility, is particularly widely applied to ophthalmology, wound dressing and artificial joints in medical treatment, and is also applied to the aspects of medical membranes, artificial kidney membranes and the like. Polyvinyl alcohol and monobutyric acid are added with some ions under an acidic environment to form double-network hydrogel, and the double-network hydrogel can be heated and solidified and attached to the surfaces of other materials under a neutral environment. The formed hydrogel has cell affinity and can load growth factors to be slowly released in vivo, but the mechanical property of the hydrogel is difficult to be used as a substitute of bone for in vivo application by singly using the hydrogel.
Polyimide is used as a special engineering material and has been widely applied to the fields of aviation, aerospace, microelectronics, nano-scale, liquid crystal, separation membranes, laser and the like. In the field of biological materials, polyimide has good biocompatibility, is closer to the elastic modulus and strength of human bone tissues, has more flexible processing mode, can be designed and prepared according to practical application, can be used as a substitute material for implanting into bones, but is biologically inert and hydrophobic, so that the preparation of the polyimide bioactive material with good bioactivity, excellent osteogenesis performance and excellent antibacterial performance has great significance.
Disclosure of Invention
The invention aims to provide a hydrogel-loaded polyimide bioactive material, a preparation method and application thereof, aiming at solving the defects of insufficient bioactivity of polyimide and mechanical property of hydrogel.
In order to achieve the purpose, the invention provides the following scheme:
one of the purposes of the invention is to provide a preparation method of a hydrogel-loaded polyimide bioactive material, which comprises the following steps: and (2) immersing the polyimide substrate into a strong alkali solution for hydrothermal treatment, coating the hydrogel on the surface of the treated polyimide substrate, then immersing the polyimide substrate in a growth factor solution, and sterilizing to obtain the hydrogel-loaded polyimide bioactive material.
Further, the polyimide is prepared by plastic processing methods such as casting, extrusion molding, powder hot press molding and the like, the shape of the prepared polyimide substrate can be designed according to actual application requirements, and specifically, after the surface of the polyimide is polished to be smooth, the polyimide is ultrasonically cleaned by a solvent and dried.
Further, the grinding is dry grinding, wet grinding or mechanical grinding; ultrasonic cleaning with acetone, ethanol, propanol, formamide or deionized water, preferably acetone, ethanol and deionized water; the drying is carried out for 1-24 h at 40-120 ℃ in a vacuum drying oven, and the optimal temperature time is 50-80 ℃ and 4-12 h.
Further, the strong alkali solution is sodium hydroxide or potassium hydroxide solution, and the concentration is 0.01-1 mol/L, preferably 0.02-0.5 mol/L; the temperature of the hydrothermal reaction is 100-150 ℃, the time is 1-12 hours, and the optimal time is 110-130 ℃, and 1-4 hours.
Further, ultrasonic cleaning is carried out again after the hydrothermal treatment, and the ultrasonic cleaning is carried out by adopting acetone, ethanol, propanol, formamide or deionized water, and the best is the acetone, the ethanol and the deionized water; after cleaning, heating to 40-100 ℃, preferably 50-90 ℃.
Further, the hydrogel is polyvinyl alcohol monobutyric acid double-network hydrogel, and the specific preparation method comprises the following steps: dissolving polyvinyl alcohol and monobutyric acid in deionized water, adjusting the pH to 1-2 by using an acidic solution, adding metal ions, and adjusting the pH to 7-8 by using an alkaline solution.
Further, the metal ion is any one of magnesium ion, zinc ion, iron ion and calcium ion, and magnesium ion is the most preferable; the mass ratio of the deionized water, the polyvinyl alcohol, the monobutyric acid and the metal ions is (90-95): 0.5-5): 0.05-0.5.
Further, the acid solution is any one of dilute hydrochloric acid, dilute sulfuric acid and dilute nitric acid, and the dilute hydrochloric acid is optimal; the alkaline solution is any one of sodium hydroxide, potassium hydroxide and ammonia water, and the sodium hydroxide solution is the best.
Further, the coating is to drop the hydrogel on the surface of the polyimide substrate, or to immerse the polyimide substrate in the hydrogel for 1-20 s, preferably 3-10 s.
Further, the growth factor is at least one of bone morphogenetic protein, basic fibroblast growth factor, insulin-like growth factor and vascular endothelial growth factor, preferably bone morphogenetic protein; the concentration of the growth factor solution is 0.01-10 mg/L, preferably 0.1-5 mg/L, and the time for soaking the growth factor solution in the growth factor solution is 10-48 hours, preferably 16-32 hours.
Further, the sterilization is any one of ultraviolet sterilization, dry heat sterilization and high pressure steam sterilization, and is most preferably ultraviolet sterilization.
The invention also aims to provide the hydrogel-loaded polyimide bioactive material prepared by the preparation method.
The invention also aims to provide application of the hydrogel-loaded polyimide bioactive material in the fields of cell culture proliferation carriers and bone tissue implantation materials.
Further, the application is specifically that the material can be used as a culture proliferation carrier of cells such as mouse embryo osteoblasts (3T3 cells), bone marrow mesenchymal stem cells (BMSC), human endothelial cells, nerves and the like in vitro, can be used as a human bone substitute in vivo, comprises an implantation material of bone tissues such as cartilage, cortical bone, meniscus, skull and the like in a human body, can also be used as an implantation material of corresponding bone tissues such as mice, rabbits, cats, dogs and the like, and can also be used as an in vivo carrier of proteins and medicaments.
The invention discloses the following technical effects:
the hydrogel-loaded polyimide bioactive material provided by the invention can be matched with corresponding dies through processing modes such as casting, extrusion molding or powder hot press molding, so that base materials with different shapes can be prepared to match different use environments, and the processing mode is flexible. The hydrophilicity of the polyimide after the hydrothermal treatment of the strong alkaline solution is greatly improved, the biocompatibility is improved, and the hydrogel attachment is facilitated. The prepared polyvinyl alcohol single-butyric acid double-network hydrogel has a simple and controllable gel forming mode, is high in curing speed, can smoothly complete the loading of the hydrogel on a polyimide substrate, can carry medicine to carry growth factors to enter a body for slow release, and greatly improves the biological activity and functionality of the material. The hydrogel-loaded polyimide bioactive material provided by the invention has the elastic modulus and the strength close to human bone tissues, has good biocompatibility, osteogenesis performance and antibacterial performance, and has very wide application value.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram of a process for preparing a hydrogel-supported polyimide bioactive material.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every intervening value, to the extent any stated value or intervening value in a stated range, and any other stated or intervening value in a stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
In the invention, the surface of the polyimide substrate is preferably polished smoothly by using sand paper, and the polishing is carried out by firstly using 300-800 meshes of sand paper, then finely grinding 1000-2000 meshes of sand paper and finally polishing 3000-5000 meshes of sand paper.
In the invention, the organic solvent used for ultrasonic cleaning is preferably acetone and ethanol, and the ultrasonic cleaning time of the acetone, the ethanol and the deionized water is respectively 8-12 min, 8-12 min and 10-12 min.
In the invention, the dropping amount of the hydrogel solution dropped on the surface of the polyimide substrate is 0.1-0.5 mL/cm2。
In the present invention, all raw materials are conventional commercial products, wherein the bone morphogenetic protein is under the brand name Bioss.
Example 1
The polyimide is processed into a bar with the diameter of 10mm by powder hot-press molding, and is cut into a thin sheet with the thickness of 1mm by a wire. And (3) polishing the surface of the polyimide substrate with sand paper, firstly carrying out primary polishing with 800-mesh sand paper, then carrying out fine polishing with 1000-mesh sand paper, and finally carrying out polishing treatment with 3000-mesh sand paper. And ultrasonic cleaning with acetone, ethanol and deionized water for 8min, 8min and 10min respectively. Drying at 80 deg.C for 6h in a vacuum drying oven, soaking in 0.1mol/L sodium hydroxide solution, and performing hydrothermal reaction at 120 deg.C for 2 h. And (4) carrying out ultrasonic cleaning again after the hydrothermal treatment is finished, wherein the cleaning steps are the same as above, and the cleaned water is reserved.
According to deionized water: polyvinyl alcohol: monobutyric acid: the mass ratio of the magnesium ions is 90: 5: 5: 0.05, 5g of polyvinyl alcohol and 5g of monobutyric acid were weighed, the polyvinyl alcohol and the monobutyric acid were dissolved in 90g of deionized water, the pH was adjusted to 1.5 with dilute hydrochloric acid, 0.3g of magnesium nitrate was added, and then the pH of the hydrogel solution was adjusted to 8 with a sodium hydroxide solution for later use.
The polyimide substrate is put into deionized water to be heated to 80 ℃, and 0.3mL/cm of polyimide substrate is dripped on the surface of the polyimide substrate when the polyimide substrate is hot2And curing the prepared hydrogel solution on a polyimide substrate after 10 seconds, putting the polyimide substrate loaded with the hydrogel into a solution containing 0.16mg/L of bone morphogenetic protein, soaking for 24 hours, and performing ultraviolet sterilization for 30min to obtain the polyimide bioactive material loaded with the hydrogel.
Example 2
The polyimide is processed into a rod with the diameter of 20mm by powder hot-press molding, and is cut into a thin sheet with the thickness of 1mm by a wire. And (3) polishing the surface of the polyimide substrate with sand paper, firstly carrying out primary polishing with 800-mesh sand paper, then carrying out fine polishing with 2000-mesh sand paper, and finally carrying out polishing treatment with 3000-mesh sand paper. And ultrasonic cleaning with acetone, ethanol and deionized water sequentially for 10min, 10min and 10min respectively. Drying at 80 deg.C for 6h in a vacuum drying oven, soaking in 0.2mol/L sodium hydroxide solution, and performing hydrothermal reaction at 120 deg.C for 2 h. And (4) carrying out ultrasonic cleaning again after the hydrothermal treatment is finished, wherein the cleaning steps are the same as above, and the cleaned water is reserved.
According to deionized water: polyvinyl alcohol: monobutyric acid: the mass ratio of the magnesium ions is 94: 3: 3: 0.05, 3g of polyvinyl alcohol and 3g of monobutyric acid are weighed, the polyvinyl alcohol and the monobutyric acid are dissolved in 94g of deionized water, then the pH value is adjusted to 2 by using dilute hydrochloric acid, then 0.3g of magnesium nitrate is added, and then the pH value of the hydrogel solution is adjusted to 7.5 by using a sodium hydroxide solution for later use.
Putting the polyimide substrate into deionized water for heating0.1mL/cm is dripped on the surface of the hot solution at 70 DEG C2And curing the hydrogel on a polyimide substrate after 8 seconds, then putting the polyimide substrate loaded with the hydrogel into a solution containing 0.24mg/L bone morphogenetic protein, soaking for 30 hours, and carrying out ultraviolet sterilization for 30 minutes to obtain the polyimide bioactive material loaded with the hydrogel.
Example 3
The polyimide is processed into a bar with the diameter of 15mm by powder hot-press molding, and is cut into a thin sheet with the thickness of 1mm by a wire. And (3) polishing the surface of the polyimide substrate with sand paper, firstly using 800-mesh sand paper for primary polishing, then using 1000-mesh and 2000-mesh sand paper for fine polishing, and finally using 5000-mesh sand paper for polishing treatment. And ultrasonic cleaning with acetone, ethanol and deionized water for 12min, 12min and 12min respectively. Drying at 70 deg.C for 8 hr in vacuum drying oven, soaking in 0.3mol/L sodium hydroxide solution, and performing hydrothermal reaction at 120 deg.C for 1 hr. And (4) carrying out ultrasonic cleaning again after the hydrothermal treatment is finished, wherein the cleaning steps are the same as above, and the cleaned water is reserved.
According to deionized water: polyvinyl alcohol: monobutyric acid: the mass ratio of the magnesium ions is 92: 4: 4: 0.05, weighing 4g of polyvinyl alcohol and 4g of monobutyric acid, dissolving the polyvinyl alcohol and the monobutyric acid in 92g of deionized water, adjusting the pH to 1.5 by using dilute hydrochloric acid, adding 0.3g of magnesium nitrate, and adjusting the pH of the hydrogel solution to 8 by using a sodium hydroxide solution for later use.
Putting the polyimide substrate into deionized water, heating to 75 ℃, and dropwise adding 0.5mL/cm on the surface of the polyimide substrate while the polyimide substrate is hot2And curing the hydrogel on a polyimide substrate after 7 seconds, then putting the polyimide substrate loaded with the hydrogel into a solution containing 0.30mg/L bone morphogenetic protein, soaking for 24 hours, and carrying out ultraviolet sterilization for 30 minutes to obtain the polyimide bioactive material loaded with the hydrogel.
Comparative example 1
The difference from example 1 is that the polyimide substrate was not subjected to hydrothermal treatment.
Comparative example 2
The difference from example 1 is that no hydrogel was added.
Comparative example 3
The difference from example 1 is that no growth factor was added.
The polyimide substrates of examples 1 to 3 and comparative examples 1 to 3 were tested for elastic modulus and hardness (hardness was measured by Hardness. Rockwell-E D785), and cell activity and alkaline phosphatase activity were measured, and the results are summarized in Table 1.
Putting a bioactive material into a forty-eight orifice plate, putting one prepared polyimide sheet into each orifice, then inoculating 8000 cells in each orifice, putting the cells into an incubator to be cultured for 1, 3 and 7 days, and evaluating the cell activity by adopting an MTT method by taking a blank polyimide sheet as a control.
The bioactive material is put into a twelve-hole plate, one prepared polyimide sheet is put into each hole, then 8000 cells are inoculated into each hole, the cells are bone marrow mesenchymal stem cells, the cells are put into an incubator to be cultured for 7 and 14 days, and the blank polyimide sheet is used as a control to detect the activity of alkaline phosphatase in the cells by using a BCI/NBT alkaline phosphatase staining kit.
Experiment for inhibiting bacteria
Respectively mixing 1 × 105CFU/mL e.coli and s.aureus were inoculated on different sample surfaces and incubated at 37 ℃ for 24 h. The surface adherent bacteria were then eluted and collected by sonication (10 min). After dilution with PBS solution, plates of LB solid medium were spread and incubated for 18 h. The formula for calculating the antibacterial rate is as follows: the antibacterial ratio (%) - (C-T)/Cx 100%. Wherein C is the average colony number CFU of the control group, and T is the CFU of the test group.
Respectively will contain 1 × 106CFU/mL of e.coli and s.aureus in 50 μ L of bacterial suspension was dropped on a plate of LB solid medium and spread uniformly. Then, the prepared hydrogel-supporting polyimide sheet and blank polyimide sheet were placed on a medium and cultured at 37 ℃ for 18 hours. The larger the antimicrobial area around the sample, the better the antimicrobial activity. The bacteriostatic rate and the diameter of the bacteriostatic ring are shown in table 2.
TABLE 1
TABLE 2
As can be seen from tables 1 and 2, the cell activity and the alkaline phosphatase activity of the example group are greatly improved compared with those of the control group, the control group has no bacteriostatic ring and almost no bacteriostatic effect, the inhibition rate of the example of the invention on e.coli and s.aureus can reach 100%, the diameter of the bacteriostatic ring of the example 3 is large, wherein the bacteriostatic ring on e.coli is about 12mm, and the bacteriostatic ring on s.aureus is about 20mm, which indicates that the bacteriostatic performance is excellent.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. A preparation method of a hydrogel-loaded polyimide bioactive material is characterized by comprising the following steps: and (2) immersing the polyimide substrate into a strong alkali solution for hydrothermal treatment, coating the hydrogel on the surface of the treated polyimide substrate, then immersing the polyimide substrate in a growth factor solution, and sterilizing to obtain the hydrogel-loaded polyimide bioactive material.
2. The preparation method of the hydrogel-supported polyimide bioactive material as claimed in claim 1, wherein the strong alkaline solution is sodium hydroxide or potassium hydroxide solution, the concentration is 0.01-1 mol/L, the temperature of the hydrothermal reaction is 100-150 ℃, and the time is 1-12 h.
3. The preparation method of the hydrogel-supported polyimide bioactive material as claimed in claim 1, wherein the hydrogel is a polyvinyl alcohol monobutyric acid double-network hydrogel, and the specific preparation method comprises: dissolving polyvinyl alcohol and monobutyric acid in deionized water, adjusting the pH to 1-2 by using an acidic solution, adding metal ions, and adjusting the pH to 7-8 by using an alkaline solution.
4. The method for preparing a hydrogel-supported polyimide bioactive material as claimed in claim 3, wherein the metal ion is any one of magnesium ion, zinc ion, iron ion and calcium ion, and the mass ratio of the deionized water, the polyvinyl alcohol, the monobutyric acid and the metal ion is (90-95): (0.5-5): (0.05-0.5).
5. The method for preparing the hydrogel-supported polyimide bioactive material as claimed in claim 3, wherein the acidic solution is any one of dilute hydrochloric acid, dilute sulfuric acid and dilute nitric acid, and the alkaline solution is any one of sodium hydroxide, potassium hydroxide and ammonia water.
6. The preparation method of the hydrogel-supported polyimide bioactive material as claimed in claim 1, wherein the coating is performed by dripping the hydrogel on the surface of the polyimide substrate or immersing the polyimide substrate in the hydrogel for 1-20 s.
7. The preparation method of the hydrogel-loaded polyimide bioactive material as claimed in claim 1, wherein the growth factor is at least one of bone morphogenetic protein, basic fibroblast growth factor, insulin-like growth factor and vascular endothelial growth factor, and the concentration of the growth factor solution is 0.01-10 mg/L.
8. The method for preparing a hydrogel-supported polyimide bioactive material as claimed in claim 1, wherein the sterilization is any one of uv sterilization, dry heat sterilization and autoclaving.
9. A hydrogel-supporting polyimide bioactive material prepared by the preparation method of any one of claims 1 to 8.
10. Use of the hydrogel-loaded polyimide bioactive material of claim 9 in the field of cell culture proliferation carriers and bone tissue implant materials.
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| CN115350334A (en) * | 2022-08-23 | 2022-11-18 | 广东工业大学 | Preparation method, product and application of polyimide-based composite aerogel material |
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