CN114081968B - Charge-reversal nano material and preparation method and application thereof - Google Patents
Charge-reversal nano material and preparation method and application thereof Download PDFInfo
- Publication number
- CN114081968B CN114081968B CN202111403762.2A CN202111403762A CN114081968B CN 114081968 B CN114081968 B CN 114081968B CN 202111403762 A CN202111403762 A CN 202111403762A CN 114081968 B CN114081968 B CN 114081968B
- Authority
- CN
- China
- Prior art keywords
- charge
- reversal
- nanomaterial
- solution
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002086 nanomaterial Substances 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 39
- 201000011510 cancer Diseases 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 13
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000003795 desorption Methods 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 6
- 238000013399 early diagnosis Methods 0.000 claims abstract description 5
- 238000004393 prognosis Methods 0.000 claims abstract description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 12
- 239000002105 nanoparticle Substances 0.000 claims description 10
- 238000001291 vacuum drying Methods 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 239000002243 precursor Substances 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical group CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 claims description 4
- 239000006087 Silane Coupling Agent Substances 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 claims description 4
- 229940040526 anhydrous sodium acetate Drugs 0.000 claims description 4
- 238000000643 oven drying Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 102000001554 Hemoglobins Human genes 0.000 claims description 3
- 108010054147 Hemoglobins Proteins 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 230000001588 bifunctional effect Effects 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 abstract description 4
- 238000012986 modification Methods 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 238000003786 synthesis reaction Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 29
- 239000000203 mixture Substances 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 3
- 239000003431 cross linking reagent Substances 0.000 description 3
- 238000007885 magnetic separation Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011528 liquid biopsy Methods 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nanotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Pathology (AREA)
- Medical Informatics (AREA)
- Cell Biology (AREA)
- Oncology (AREA)
- Immunology (AREA)
- Manufacturing & Machinery (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Composite Materials (AREA)
- Materials Engineering (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a charge inversion type nano material, a preparation method and application thereof, wherein the material takes ferroferric oxide as a core, and can realize efficient capture and adjustable desorption on cancer cells after modification. Compared with the prior art, the invention has the following advantages: (1) The synthesis condition of the charge-reversal nano material is mild, and the modification path is simple; (2) The nano material can be regulated and controlled in the capturing and desorbing processes for cancer cells, and the regulation and control mode is simple, and only the pH value of the environment is required to be changed; (3) The nano material has wide application range, and is applicable to early diagnosis, personalized treatment, prognosis and the like of tumors.
Description
Technical Field
The invention belongs to the technical field of nano materials, and relates to a functional nano material for capturing cancer cells, in particular to a charge inversion type nano material, a preparation method and application thereof.
Background
The liquid biopsy method is often adopted for early diagnosis, intraoperative and postoperative monitoring of cancer patients in clinic, and has the advantages of no wound, convenient sampling, high sensitivity and the like. Researchers want to efficiently enrich circulating tumor cells in body fluid, primarily diagnose cancers through the number of cancer cells, and simultaneously realize personalized diagnosis and treatment of patients by utilizing gene information in the circulating tumor cells. Currently, there are methods for positively capturing cancer cells, but how to achieve controlled desorption after capture is a currently urgent problem.
Disclosure of Invention
The technical problems to be solved are as follows: in order to overcome the defects of the prior art, the invention provides a charge-reversal type nano material capable of realizing efficient capture and desorption of a controllable nano material for cancer cells, and a preparation method and application thereof.
The technical scheme is as follows: a method of preparing a charge-reversed nanomaterial, the method comprising the steps of:
s1, dissolving ferroferric oxide nanoparticle precursor and 1-5mg of anhydrous sodium acetate in ethylene glycol, wherein the concentration of the precursor is 10-50mg/mL, and obtaining solution A; wherein the precursor is FeCl 3 ·6H 2 O；
S2, placing the solution A in a vacuum reaction kettle, and reacting for 6-15 hours at the temperature of 150-250 ℃ to obtain a ferroferric oxide core;
s3, mixing and stirring the ferroferric oxide core and a tetraethyl silicate solution with the concentration of 0.3-5mg/mL for 8-24 hours to obtain a solution B;
s4, mixing and stirring 0.1-5mg/mL of the solution B with 0.1-3mL of the silane coupling agent for 10-24 hours to obtain a solution C;
s5, placing the solution C in a vacuum drying oven, and vacuum drying at 30-150 ℃ for 10-48 hours to obtain nano particles;
s6, taking the nano particles prepared in the S5, excessive bifunctional reagents (such as EDC and NHS) and substances with isoelectric points between 6 and 8, reversing and mixing uniformly for 1-24 hours, and performing magnetic separation to obtain the charge-reversal nano material.
Preferably, the silane coupling agent in S4 is 3-aminopropyl triethoxysilane.
Preferably, the substance with isoelectric point between 6-8 in S5 is bovine hemoglobin or chitosan.
The charge-reversal nanomaterial obtained by any of the above methods.
The application of the charge-reversal nano material in capturing cancer cells.
Preferably, the reverse nano material positively captures cancer cells, and potential reversal of the material is regulated through isoelectric point change after capturing so as to realize desorption between the cancer cells and the reverse nano material.
Preferably, the reverse nanomaterial undergoes a potential reversal at a pH of 5 to 9.
The charge-reversal nano material is applied to the preparation of a kit for early diagnosis, personalized treatment or prognosis monitoring of tumors.
The charge-reversal nano material disclosed by the invention has the following principle that cancer cells are captured and desorbed: the charge inversion type nano material takes ferroferric oxide as a core, has magnetism, and is modified by surface coupling to form a substance with isoelectric point between 6 and 8, so that the charge inversion of the nano material is realized by regulating and controlling pH between 5 and 9. Specifically, the charge-reversal nano material of the application positively captures cancer cells on the surface in body fluid (such as PBS or DMEM in an environment with pH of 5-7), and then the surface charge of the nano material is reversed to be negative by adjusting the pH value of the environment, so that the nano material is mutually repelled with the negative on the surface of the cancer cells, and the cancer cells are caused to fall off to achieve the desorption effect.
The beneficial effects are that: (1) The synthesis condition of the charge-reversal nano material is mild, and the modification path is simple; (2) The nano material can be regulated and controlled in the capturing and desorbing processes for cancer cells, and the regulation and control mode is simple, and only the pH value of the environment is required to be changed; (3) The nano material has wide application range, and is applicable to early diagnosis, personalized treatment, prognosis and the like of tumors.
Drawings
FIG. 1 is a transmission electron microscope image of the charge-reversal nanomaterial synthesized in example 1;
FIG. 2 is a Diff-Quik plot of the charge-reversed nanomaterial synthesized in example 1 capturing cancer cells;
FIG. 3 is a Diff-Quik plot of the charge-reversed nanomaterial synthesized in example 1 desorbing cancer cells;
FIG. 4 shows the capture efficiency and dissociation rate of the charge-reversed nano-materials synthesized in example 1 on cancer cells.
Detailed Description
The following examples further illustrate the invention but are not to be construed as limiting the invention. Modifications and substitutions to the method, steps or conditions of the invention without departing from the spirit and nature of the invention are intended to be within the scope of the invention. The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated.
Example 1
A method of preparing a charge-reversed nanomaterial, the method comprising the steps of:
s1, weighing 50-2000mg FeCl 3 ·6H 2 O,1-5mg of anhydrous sodium acetate is added into 10-60mL of ethylene glycol, and the mixture is stirred and mixed uniformly;
s2, pouring the mixture into a vacuum reaction kettle, reacting for 6-15 hours at the temperature of 150-250 ℃ to obtain ferroferric oxide cores, and performing magnetic separation;
s3, taking 80-300mg of the magnetic core, adding 30-150 mu L of tetraethyl silicate, stirring for 8-24 hours by ultrasonic, and separating magnetically;
s4, taking 0.1mg-30mg of the nano particles, adding 800-1500 mu L of 3-aminopropyl triethoxysilane, ultrasonically stirring for 10-24 hours, and magnetically separating;
s5, placing the solution C in a vacuum drying oven, and vacuum drying at 30-150 ℃ for 10-48h;
s6, taking 0.1mg-5mg of the nano particles, adding excessive bifunctional reagents such as EDC and NHS, simultaneously adding bovine hemoglobin, and mixing for 1-24 hours in a reverse way.
As shown in FIG. 1, the transmission electron microscope picture of the charge-reversal type nano material synthesized in the embodiment is spherical with uniform size, and the size is 300-500nm.
The charge-reversal nanomaterial prepared in example 1 was used to prepare a 1mg/mL solution to 10 ^ 4-10 ^ The capture of cancer cells is carried out by adding 20-500 mu L of nano-material into cancer cells of the order of magnitude of 6. As shown in fig. 2, the charge-reversal nanomaterial synthesized in this embodiment can be tightly connected to cancer cells and effectively capture cancer cells.
As shown in FIG. 3, cancer cells can be captured at pH 5-6.8, and can be released when the pH of the environment is adjusted to 7-9, so that the visual field is clean, and most of materials are removed, thus obtaining pure cancer cells.
Example 2
S1, 0.1-10g chitosan and 80-200mL 2% acetic acid are stirred and mixed uniformly for 0.5-24h, 0.1-20g/mL phthalic acid (dissolved in acetone) is added, and the reaction is carried out for 5-24h. Drying in a vacuum drying oven at 25-80deg.C to obtain product A.
S2, weighing 50-2000mg FeCl 3 ·H 2 O,1-5mg of anhydrous sodium acetate is added into 10-60mL of ethylene glycol, and the mixture is stirred and mixed uniformly;
s3, pouring the mixture into a vacuum reaction kettle, reacting for 6-15 hours at the temperature of 150-250 ℃ to obtain ferroferric oxide cores, and performing magnetic separation;
s4, taking 80-300mg of the magnetic core, adding 30-150 mu L of tetraethyl silicate, stirring for 8-24 hours by ultrasonic, and separating magnetically;
s5, taking 0.1mg-30mg of the nano particles, adding 800-1500 mu L of 3-aminopropyl triethoxysilane, ultrasonically stirring for 10-20 hours, and magnetically separating;
s6, placing the solution C in a vacuum drying oven, and vacuum drying at 30-150 ℃ for 10-48h.
S7, taking 0.1mg-5mg of the nano particles, adding excessive bifunctional reagents such as EDC and NHS, simultaneously adding modified chitosan A, and mixing the mixture for 1 to 24 hours in a reverse way.
The charge-reversal nanomaterial prepared in example 2 was used to prepare a 1mg/mL solution to 10 ^ 4-10 ^ The capture of cancer cells is carried out by adding 20-500 mu L of nano-material into cancer cells of the order of magnitude of 6. Experimental effect reference example 1.
Example 3
Charge-reversal nanomaterial capture and release cancer cell efficiency experiments:
the charge-reversal nanomaterial prepared in example 1 was used to prepare a 1mg/mL solution to 10 ^ 4-10 ^ The capture of cancer cells is carried out by adding 20-500 mu L of nano-material into cancer cells of the order of magnitude of 6. Cancer cells can be captured and counted when the pH of PBS is adjusted to 5-6.8; cancer cells are released and counted when the pH of the environment is adjusted to 7-9.
The capture rate and dissociation rate of the material for cancer cells are shown in figure 4.
Claims (6)
1. A method for preparing a charge-reversal nanomaterial, the method comprising the steps of:
s1, dissolving ferroferric oxide nanoparticle precursor and 1-5mg of anhydrous sodium acetate in ethylene glycol, wherein the concentration of the precursor is 10-50mg/mL, and obtaining solution A; wherein the precursor is FeCl 3 ·6H 2 O；
S2, placing the solution A in a vacuum reaction kettle, and reacting for 6-15 hours at the temperature of 150-250 ℃ to obtain a ferroferric oxide core;
s3, mixing and stirring the ferroferric oxide core and a tetraethyl silicate solution with the concentration of 0.3-5mg/mL for 8-24 hours to obtain a solution B;
s4, mixing and stirring 0.1-5mg/mL of the solution B with 0.1-3mL of the silane coupling agent for 10-24 hours to obtain a solution C; the silane coupling agent is 3-aminopropyl triethoxysilane;
s5, placing the solution C in a vacuum drying oven, and vacuum drying at 30-150 ℃ for 10-48 hours to obtain nano particles;
s6, taking the nano particles prepared in the S5, excessive bifunctional reagent and substances with isoelectric points between 6 and 8, reversing and uniformly mixing for 1-24 hours, and magnetically separating to obtain the charge-reversal nano material; wherein the material with isoelectric point between 6-8 is bovine hemoglobin.
2. A charge-reversed nanomaterial made by the method of claim 1.
3. Use of the charge-reversed nanomaterial of claim 2 for the preparation of a cancer cell-capturing nanomaterial.
4. The use according to claim 3, wherein the reverse nanomaterial positively traps cancer cells, and the potential reversal of the material is modulated by isoelectric point change after trapping to effect desorption therebetween.
5. The use according to claim 4, wherein the reverse nanomaterial undergoes a potential reversal at a pH of 5-9.
6. The use of the charge-reversal nanomaterial of claim 2 for preparing a kit for early diagnosis, personalized treatment or prognosis monitoring of tumors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111403762.2A CN114081968B (en) | 2021-11-24 | 2021-11-24 | Charge-reversal nano material and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111403762.2A CN114081968B (en) | 2021-11-24 | 2021-11-24 | Charge-reversal nano material and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114081968A CN114081968A (en) | 2022-02-25 |
| CN114081968B true CN114081968B (en) | 2023-06-23 |
Family
ID=80303874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111403762.2A Active CN114081968B (en) | 2021-11-24 | 2021-11-24 | Charge-reversal nano material and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114081968B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105418941A (en) * | 2015-12-31 | 2016-03-23 | 北京师范大学 | Nanometer composite material, preparing method and application |
| CN109369975A (en) * | 2018-11-12 | 2019-02-22 | 南京工业大学 | A kind of preparation method of chitosan magnetic-silicon dioxide composite aerogel |
| CN111603568A (en) * | 2020-05-29 | 2020-09-01 | 南通大学 | Charge reversal type polypeptide composite nano-drug and preparation method and application thereof |
| CN113233563A (en) * | 2021-03-17 | 2021-08-10 | 重庆大学 | Preparation method and application of cationic chitosan-based magnetic flocculant initiated by low-pressure ultraviolet light |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112394167A (en) * | 2019-08-19 | 2021-02-23 | 中国科学院苏州纳米技术与纳米仿生研究所 | Fluorescent nano magnetic bead for capturing and identifying CTCs (biological chemical centers), and preparation method and application thereof |
-
2021
- 2021-11-24 CN CN202111403762.2A patent/CN114081968B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105418941A (en) * | 2015-12-31 | 2016-03-23 | 北京师范大学 | Nanometer composite material, preparing method and application |
| CN109369975A (en) * | 2018-11-12 | 2019-02-22 | 南京工业大学 | A kind of preparation method of chitosan magnetic-silicon dioxide composite aerogel |
| CN111603568A (en) * | 2020-05-29 | 2020-09-01 | 南通大学 | Charge reversal type polypeptide composite nano-drug and preparation method and application thereof |
| CN113233563A (en) * | 2021-03-17 | 2021-08-10 | 重庆大学 | Preparation method and application of cationic chitosan-based magnetic flocculant initiated by low-pressure ultraviolet light |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114081968A (en) | 2022-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5569837B2 (en) | Method for producing surface-coated inorganic particles | |
| Lian et al. | Synthesis of magnetite nanorods and porous hematite nanorods | |
| JP6788686B2 (en) | A method for producing a superparamagnetic nanocomposite and a superparamagnetic nanocomposite produced using the method. | |
| US5776360A (en) | Highly disperse magnetic metal oxide particles, processes for their preparation and their use | |
| Kim et al. | Scalable solvothermal synthesis of superparamagnetic Fe3O4 nanoclusters for bioseparation and theragnostic probes | |
| US9127168B2 (en) | Magnetic iron oxide fine particles, and magnetic particle-containing water dispersion and process for producing the same | |
| CN101417822B (en) | Method for preparing super paramagnetic mesoporous ferriferrous oxide nano particle | |
| EP2121040A1 (en) | Compositions containing metal oxide particles and their uses | |
| JPWO2004083124A1 (en) | Noble metal / magnetic metal oxide composite fine particles and production method thereof | |
| CN1283586C (en) | Method for preparing cobalt ferrite by coprecipitation | |
| CN112011513B (en) | Method for capturing high-purity circulating tumor cells based on bioorthogonal chemical method | |
| JP2013019889A (en) | Magnetic particle and manufacturing method thereof | |
| CN111072070A (en) | Preparation method of high-saturation magnetization superparamagnetic porous ferrite microspheres | |
| Wang et al. | Recent advances in development of functional magnetic adsorbents for selective separation of proteins/peptides | |
| Cano et al. | Self-assembly of a superparamagnetic raspberry-like silica/iron oxide nanocomposite using epoxy–amine coupling chemistry | |
| CN114081968B (en) | Charge-reversal nano material and preparation method and application thereof | |
| JP2008019162A (en) | Noble metal-magnetic metal oxide composite particle and method for producing the same | |
| CN107802845B (en) | Method for phase conversion of hydrophobic nanoparticles by using silk fibroin molecules | |
| CN110507829B (en) | Preparation method and application of iron-tungsten composite oxide nanocrystalline cluster | |
| CN105381475B (en) | A kind of magnetic resonance imaging contrast and the preparation method and application thereof | |
| JP2014156411A (en) | Composite magnetic particulate powder, and dispersion | |
| CN107224588A (en) | A kind of preparation method for the pharmaceutical carrier answered with magnetic pH value double-bang firecracker | |
| KR101991912B1 (en) | Mri contrast agent including t1 contrast material coated on surface of nanoparticle support | |
| JP2007084407A (en) | Metal colloidal solution and method for its preparation | |
| Trekker et al. | Synthesis of PEGylated magnetic nanoparticles with different core sizes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |