CN114073315A - Probiotic preparation - Google Patents

Probiotic preparation Download PDF

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Publication number
CN114073315A
CN114073315A CN202010793628.7A CN202010793628A CN114073315A CN 114073315 A CN114073315 A CN 114073315A CN 202010793628 A CN202010793628 A CN 202010793628A CN 114073315 A CN114073315 A CN 114073315A
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parts
vitamin
lactobacillus
bifidobacterium
gamma
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Inventor
邹文林戈
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Hubei Houpin Biotechnology Co ltd
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Hubei Houpin Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/158—Milk preparations; Milk powder or milk powder preparations containing additives containing vitamins or antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/175—Amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A61K31/51—Thiamines, e.g. vitamin B1
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7135—Compounds containing heavy metals
    • A61K31/714—Cobalamins, e.g. cyanocobalamin, i.e. vitamin B12
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/733—Fructosans, e.g. inulin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/745—Bifidobacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/20—Hypnotics; Sedatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/123—Bulgaricus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/125—Casei
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/173—Reuteri
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/175—Rhamnosus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/513—Adolescentes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/515—Animalis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/517—Bifidum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/529—Infantis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

Abstract

The invention relates to a probiotic preparation which comprises the following raw materials in parts by weight: 1-10 parts of composite probiotics, 20-80 parts of inulin, 20-30 parts of full cream milk powder, 3-17 parts of gamma-aminobutyric acid, 10.01-5 parts of vitamin B, 120.01-5 parts of vitamin B and 0.1-5 parts of edible essence. Compared with the prior art, the invention can relieve emotion and help sleep more effectively.

Description

Probiotic preparation
Technical Field
The invention relates to the technical field of probiotics, and particularly relates to a probiotic preparation.
Background
Modern people have fast pace of life and high working pressure. It is commonly in sub-health situations such as anxiety, insomnia and poor sleep quality. The lack of sleep can cause many health problems, seriously affect the quality of life, and easily cause physical discomfort, such as: asthenia, muscular soreness, drowsiness, asthenia, heart deficiency, short breath, conduct disorder, and aging; in the heart will: causing depression, slow response, low mood and hypomnesis. The lack of sleep for a long time brings serious harm to health and heavy burden to individuals and families, so that the study of health products with mood relieving and sleep aiding effects is of great significance.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the above-mentioned problems in the prior art. To this end, it is an object of the present invention to propose a more effective probiotic preparation that soothes mood and aids sleep.
The technical scheme for solving the technical problems is as follows: a probiotic preparation comprises the following raw materials in parts by weight: 1-10 parts of composite probiotics, 20-80 parts of inulin, 20-30 parts of full cream milk powder, 3-17 parts of gamma-aminobutyric acid, 10.01-5 parts of vitamin B, 120.01-5 parts of vitamin B and 0.1-5 parts of edible essence.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the probiotic preparation comprises the following raw materials in parts by weight: 1 part of composite probiotics, 80 parts of inulin, 20 parts of full cream milk powder, 3 parts of gamma-aminobutyric acid, 10.01 parts of vitamin B, 120.01 parts of vitamin B and 0.1 part of edible essence.
Further, the probiotic preparation comprises the following raw materials in parts by weight: 5 parts of composite probiotics, 50 parts of inulin, 25 parts of full cream milk powder, 10 parts of gamma-aminobutyric acid, 12.8 parts of vitamin B, 122.8 parts of vitamin B and 2.5 parts of edible essence.
Further, the probiotic preparation comprises the following raw materials in parts by weight: 10 parts of composite probiotics, 20 parts of inulin, 30 parts of full cream milk powder, 17 parts of gamma-aminobutyric acid, 15 parts of vitamin B, 125 parts of vitamin B and 5 parts of edible essence.
Further, the composite probiotics are lactobacillus or/and bifidobacterium.
Further, the lactobacillus is one or more of lactobacillus plantarum, lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri and lactobacillus casei.
Further, the bifidobacterium is one or more of animal bifidobacterium, bifidobacterium longum, bifidobacterium bifidum, bifidobacterium infantis and bifidobacterium adolescentis.
The invention has the beneficial effects that:
compounding probiotics: regulating gastrointestinal discomfort, enhancing immunity, and improving ecological balance of intestinal flora;
inulin: the dietary fiber can promote intestinal peristalsis, prevent constipation, control blood fat, reduce blood sugar value and reduce blood sugar concentration;
whole milk powder: can increase energy, supplement protein, and enhance immunity;
gamma-aminobutyric acid: can promote brain activation, strengthen brain, promote intelligence, resist epilepsy, help sleep, care skin, delay brain aging function, supplement inhibitory neurotransmitter of human body, and has good effect of lowering blood pressure;
by adding gamma-aminobutyric acid into the probiotic preparation and matching with vitamin B1, vitamin B1 generated by metabolism of probiotics in intestinal tracts and serotonin substances, the emotion can be relieved more effectively and sleep is helped.
Detailed Description
The principles and features of this invention are described below in conjunction with examples which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
The preparation method of the composite probiotics comprises the following steps:
preparing strains: carrying out liquid culture and expanding culture on the primary original strain, and subpackaging to obtain a working strain;
production of probiotics: the fermentation tank with high sealing performance and high precision is used for preparation;
identification of probiotics: respectively extracting specific deoxyribonucleic acid of each probiotic by using a standard purification method, amplifying by using polymerase chain reaction to obtain a 16S rRNA gene fragment of each probiotic, sending the amplified fragment to a commercial sequencing mechanism for sequencing, determining whether a detected series is the specific probiotic or not by using gene analysis software and taking the 16S rRNA gene of each probiotic as a standard, wherein the universal primers for amplifying by using the polymerase chain reaction are as follows:
Lac1:AACAGGATTAGATACCCTGGTAGTC；
Lac2:GCGGTGTGTACAAGGCCCGGGAACG；
and (3) viable count detection: diluting the fermented bacteria liquid to three different dilution concentrations of 10, 100 and 1000 probiotics per 1 ml, taking out 1 ml of the diluted bacteria liquid respectively, paving the diluted bacteria liquid into a sterilized blank culture dish, pouring 10-20 ml of MRS culture medium for anaerobic culture for 24 hours, counting the number of bacterial colonies in the culture dish, selecting the culture dish with proper concentration according to the three dilution degrees, and obtaining the bacterial concentration in the original bacteria liquid by the count times and the dilution times of about 100 bacterial colonies in each culture dish;
and (3) freeze drying: centrifuging the fermented bacteria liquid to obtain bacteria mud; and (3) placing the bacterial sludge in a freeze dryer for freeze drying for 18-32h under negative pressure at low temperature to prepare probiotic freeze-dried powder, namely the composite probiotics.
Example 1:
a probiotic preparation comprises the following raw materials in parts by weight: 1 part of composite probiotics, 80 parts of inulin, 20 parts of full cream milk powder, 3 parts of gamma-aminobutyric acid, 10.01 parts of vitamin B, 120.01 parts of vitamin B and 0.1 part of edible essence.
In the above embodiment, the composite probiotics are lactobacillus or/and bifidobacterium.
In the above embodiments, the lactobacillus is one or more of lactobacillus plantarum, lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, and lactobacillus casei.
In the above embodiments, the bifidobacterium is one or more of bifidobacterium animalis, bifidobacterium longum, bifidobacterium bifidum, bifidobacterium infantis and bifidobacterium adolescentis.
According to the embodiment, the probiotic preparation is added with the gamma-aminobutyric acid, and the gamma-aminobutyric acid is matched with the vitamin B1, the vitamin B1 generated by metabolism of probiotics in intestinal tracts and serotonin substances, so that the emotion can be relieved more effectively, and the sleep is helped.
Example 2:
a probiotic preparation comprises the following raw materials in parts by weight: 5 parts of composite probiotics, 50 parts of inulin, 25 parts of full cream milk powder, 10 parts of gamma-aminobutyric acid, 12.8 parts of vitamin B, 122.8 parts of vitamin B and 2.5 parts of edible essence.
In the above embodiment, the composite probiotics are lactobacillus or/and bifidobacterium.
In the above embodiments, the lactobacillus is one or more of lactobacillus plantarum, lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, and lactobacillus casei.
In the above embodiments, the bifidobacterium is one or more of bifidobacterium animalis, bifidobacterium longum, bifidobacterium bifidum, bifidobacterium infantis and bifidobacterium adolescentis.
According to the embodiment, the probiotic preparation is added with the gamma-aminobutyric acid, and the gamma-aminobutyric acid is matched with the vitamin B1, the vitamin B1 generated by metabolism of probiotics in intestinal tracts and serotonin substances, so that the emotion can be relieved more effectively, and the sleep is helped.
Example 3:
a probiotic preparation comprises the following raw materials in parts by weight: 10 parts of composite probiotics, 20 parts of inulin, 30 parts of full cream milk powder, 17 parts of gamma-aminobutyric acid, 15 parts of vitamin B, 125 parts of vitamin B and 5 parts of edible essence.
In the above embodiment, the composite probiotics are lactobacillus or/and bifidobacterium.
In the above embodiments, the lactobacillus is one or more of lactobacillus plantarum, lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, and lactobacillus casei.
In the above embodiments, the bifidobacterium is one or more of bifidobacterium animalis, bifidobacterium longum, bifidobacterium bifidum, bifidobacterium infantis and bifidobacterium adolescentis.
According to the embodiment, the probiotic preparation is added with the gamma-aminobutyric acid, and the gamma-aminobutyric acid is matched with the vitamin B1, the vitamin B1 generated by metabolism of probiotics in intestinal tracts and serotonin substances, so that the emotion can be relieved more effectively, and the sleep is helped.
Firstly, performing a taste test on the probiotic preparation prepared according to the three embodiments, distributing the probiotic preparation to different people of 20-60 years old for tasting, and performing subjective evaluation on 50 people in total; the following data were obtained:
Figure BDA0002624655330000051
Figure BDA0002624655330000061
Figure BDA0002624655330000071
the mouthfeel experiment can show that: the subjects generally believe that the composition tastes slightly sweet and acceptable, has a slight milk flavor, and generally can accept the taste.
Secondly, performing a taste test on the probiotic preparation prepared according to the three embodiments; subject: the main symptoms of a population without organic lesions and with mental sub-health are as follows: restlessness, irritability, fatigue, difficulty in falling asleep. Total 50, male and female ratio 1: 1. according to different age groups, the two groups are divided into an experimental group and a control group on average; double blind tests were performed: the experimental group takes 6g of the composition powder with warm water every day for two weeks; the control group took 6g of placebo, i.e. 6g of a mixture of maltodextrin and glucose, daily for two weeks; the participating testers do not know the nature of the taken sample in advance; after the trial, the experimental results were recorded to obtain the following data:
experimental group (25 persons)
Figure BDA0002624655330000072
Figure BDA0002624655330000081
Control group (25 persons)
Figure BDA0002624655330000082
Figure BDA0002624655330000091
Figure BDA0002624655330000101
The statistical results of the above experiments can be used to obtain: after 19 of 25 people in the experimental group generally react to take the medicine, the emotion is obviously improved, and the pressure feeling is reduced; 15 people react easily to fall asleep, the sleep quality is improved, and the people are full of energy after being awake; 3 people had slightly improved but not obvious emotional response; 3 people have no change in the reaction mood. In the control group, 4 of 25 people had slight improvement in the emotional response, and 21 people had no change in the emotional response.
The experimental results show that the composition can effectively condition emotion, relieve mental stress, help fall asleep and improve sleep quality.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (7)

1. A probiotic formulation characterized by: the composition comprises the following raw materials in parts by weight: 1-10 parts of composite probiotics, 20-80 parts of inulin, 20-30 parts of full cream milk powder, 3-17 parts of gamma-aminobutyric acid, 10.01-5 parts of vitamin B, 120.01-5 parts of vitamin B and 0.1-5 parts of edible essence.
2. A probiotic formulation according to claim 1, characterized in that: the composition comprises the following raw materials in parts by weight: 1 part of composite probiotics, 80 parts of inulin, 20 parts of full cream milk powder, 3 parts of gamma-aminobutyric acid, 10.01 parts of vitamin B, 120.01 parts of vitamin B and 0.1 part of edible essence.
3. A probiotic formulation according to claim 1, characterized in that: the composition comprises the following raw materials in parts by weight: 5 parts of composite probiotics, 50 parts of inulin, 25 parts of full cream milk powder, 10 parts of gamma-aminobutyric acid, 12.8 parts of vitamin B, 122.8 parts of vitamin B and 2.5 parts of edible essence.
4. A probiotic formulation according to claim 1, characterized in that: the composition comprises the following raw materials in parts by weight: 10 parts of composite probiotics, 20 parts of inulin, 30 parts of full cream milk powder, 17 parts of gamma-aminobutyric acid, 15 parts of vitamin B, 125 parts of vitamin B and 5 parts of edible essence.
5. A probiotic formulation according to any one of claims 1 to 4, characterized in that: the composite probiotics are lactobacillus or/and bifidobacterium.
6. A probiotic formulation according to claim 5, characterized in that: the lactobacillus is one or more of lactobacillus plantarum, lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri and lactobacillus casei.
7. A probiotic formulation according to claim 5, characterized in that: the Bifidobacterium is one or more of animal Bifidobacterium, Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium infantis, and Bifidobacterium adolescentis.
CN202010793628.7A 2020-08-10 2020-08-10 Probiotic preparation Pending CN114073315A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115226773A (en) * 2022-06-29 2022-10-25 湖北明慧健康科技有限责任公司 Composite probiotic sleep-aiding solid beverage and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115226773A (en) * 2022-06-29 2022-10-25 湖北明慧健康科技有限责任公司 Composite probiotic sleep-aiding solid beverage and preparation method thereof

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