CN114062546A - Method for simultaneously screening 8 illegal coloring agents in muscle-relaxing and blood-circulation-promoting tablets - Google Patents
Method for simultaneously screening 8 illegal coloring agents in muscle-relaxing and blood-circulation-promoting tablets Download PDFInfo
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- CN114062546A CN114062546A CN202111350123.4A CN202111350123A CN114062546A CN 114062546 A CN114062546 A CN 114062546A CN 202111350123 A CN202111350123 A CN 202111350123A CN 114062546 A CN114062546 A CN 114062546A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000003086 colorant Substances 0.000 title claims abstract description 18
- 238000012216 screening Methods 0.000 title claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 38
- 239000000243 solution Substances 0.000 claims abstract description 24
- PEAGNRWWSMMRPZ-UHFFFAOYSA-L woodstain scarlet Chemical compound [Na+].[Na+].OC1=CC=C2C=C(S([O-])(=O)=O)C=C(S([O-])(=O)=O)C2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 PEAGNRWWSMMRPZ-UHFFFAOYSA-L 0.000 claims abstract description 13
- 230000017531 blood circulation Effects 0.000 claims abstract description 9
- 210000003205 muscle Anatomy 0.000 claims abstract description 9
- 230000002040 relaxant effect Effects 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 238000004043 dyeing Methods 0.000 claims abstract description 7
- 239000012085 test solution Substances 0.000 claims abstract description 7
- 230000004936 stimulating effect Effects 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 9
- 235000012730 carminic acid Nutrition 0.000 claims description 9
- 239000012488 sample solution Substances 0.000 claims description 9
- 235000005979 Citrus limon Nutrition 0.000 claims description 7
- 235000012733 azorubine Nutrition 0.000 claims description 7
- TVWOWDDBXAFQDG-DQRAZIAOSA-N azorubine Chemical compound C1=CC=C2C(\N=N/C3=C(C4=CC=CC=C4C(=C3)S(O)(=O)=O)O)=CC=C(S(O)(=O)=O)C2=C1 TVWOWDDBXAFQDG-DQRAZIAOSA-N 0.000 claims description 7
- IINNWAYUJNWZRM-UHFFFAOYSA-L erythrosin B Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 IINNWAYUJNWZRM-UHFFFAOYSA-L 0.000 claims description 7
- 235000012732 erythrosine Nutrition 0.000 claims description 7
- 239000004174 erythrosine Substances 0.000 claims description 7
- 229940011411 erythrosine Drugs 0.000 claims description 7
- CQPFMGBJSMSXLP-UHFFFAOYSA-M acid orange 7 Chemical compound [Na+].OC1=CC=C2C=CC=CC2=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 CQPFMGBJSMSXLP-UHFFFAOYSA-M 0.000 claims description 6
- 235000012741 allura red AC Nutrition 0.000 claims description 4
- 239000004191 allura red AC Substances 0.000 claims description 4
- CEZCCHQBSQPRMU-UHFFFAOYSA-L chembl174821 Chemical compound [Na+].[Na+].COC1=CC(S([O-])(=O)=O)=C(C)C=C1N=NC1=C(O)C=CC2=CC(S([O-])(=O)=O)=CC=C12 CEZCCHQBSQPRMU-UHFFFAOYSA-L 0.000 claims description 4
- 238000000589 high-performance liquid chromatography-mass spectrometry Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 3
- 239000005695 Ammonium acetate Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 238000009210 therapy by ultrasound Methods 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 244000248349 Citrus limon Species 0.000 claims 2
- 229940043376 ammonium acetate Drugs 0.000 claims 1
- 235000019257 ammonium acetate Nutrition 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000010186 staining Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 7
- 229940079593 drug Drugs 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 244000020518 Carthamus tinctorius Species 0.000 description 5
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 5
- 244000131522 Citrus pyriformis Species 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 230000001737 promoting effect Effects 0.000 description 3
- 210000002435 tendon Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- STZCRXQWRGQSJD-UHFFFAOYSA-M sodium;4-[[4-(dimethylamino)phenyl]diazenyl]benzenesulfonate Chemical compound [Na+].C1=CC(N(C)C)=CC=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 STZCRXQWRGQSJD-UHFFFAOYSA-M 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- YYYARFHFWYKNLF-UHFFFAOYSA-N 4-[(2,4-dimethylphenyl)diazenyl]-3-hydroxynaphthalene-2,7-disulfonic acid Chemical compound CC1=CC(C)=CC=C1N=NC1=C(O)C(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=C12 YYYARFHFWYKNLF-UHFFFAOYSA-N 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 208000025940 Back injury Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 235000014066 European mistletoe Nutrition 0.000 description 1
- 241000574434 Jasminum laurifolium Species 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 241000195954 Lycopodium clavatum Species 0.000 description 1
- 241000729196 Lycopus lucidus Species 0.000 description 1
- 208000000112 Myalgia Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000152640 Rhipsalis cassutha Species 0.000 description 1
- 235000012300 Rhipsalis cassutha Nutrition 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 241000913745 Spatholobus Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000004176 azorubin Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- YSVBPNGJESBVRM-UHFFFAOYSA-L disodium;4-[(1-oxido-4-sulfonaphthalen-2-yl)diazenyl]naphthalene-1-sulfonate Chemical compound [Na+].[Na+].C1=CC=C2C(N=NC3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)O)=CC=C(S([O-])(=O)=O)C2=C1 YSVBPNGJESBVRM-UHFFFAOYSA-L 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000001040 synthetic pigment Substances 0.000 description 1
- 235000012756 tartrazine Nutrition 0.000 description 1
- 239000004149 tartrazine Substances 0.000 description 1
- UJMBCXLDXJUMFB-GLCFPVLVSA-K tartrazine Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)C1=NN(C=2C=CC(=CC=2)S([O-])(=O)=O)C(=O)C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 UJMBCXLDXJUMFB-GLCFPVLVSA-K 0.000 description 1
- 229960000943 tartrazine Drugs 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
Abstract
The invention discloses a method for simultaneously screening 8 illegal coloring agents in a tablet for relaxing muscles and stimulating blood circulation, which belongs to the technical field of coloring agent screening and comprises the following steps: (1) solution preparation: preparing a contrast reagent solution; preparing a test solution; a negative solution; (2) chromatographic conditions; (3) and (4) measuring. The application adopts an HPLC-DAD method to detect 8 coloring agents in the tendon-relaxing and blood-circulation-promoting tablets, the established method inspects 46 batches of tendon-relaxing and blood-circulation-promoting tablet samples, wherein 4 batches of acid red 73 are detected, which indicates that the use condition of chemical coloring agents in Chinese patent medicines is still severe, and the attention to illegal dyeing conditions in Chinese medicinal materials and Chinese medicinal decoction pieces and the supervision strength should be increased so as to ensure the safety and effectiveness of the medicines.
Description
Technical Field
The invention belongs to the technical field of coloring agent screening, and particularly relates to a method for simultaneously screening 8 illegal coloring agents in a tablet for relaxing muscles and tendons and promoting blood circulation.
Background
The muscle-relaxing and blood-circulation-promoting tablet is a commonly used Chinese patent medicine variety and is collected in the thirteenth volume of Chinese medicinal prescription preparations in the drug Standard of the Ministry of health, the current execution standard has the national drug administration Standard [1], and the prescription consists of safflower, vinegar-processed rhizoma cyperi, scalded rhizoma cibotii, cortex periplocae, Chinese starjasmine stem, common clubmoss herb, hiraute shiny bugleweed herb, mistletoe, suberect spatholobus stem and calcined natural copper; has the effects of relaxing muscles and tendons, activating collateral flow, promoting blood circulation, and removing blood stasis; can be used for treating arthralgia and myalgia, spasm of limbs, soreness of waist and back, and traumatic injury. Safflower in the prescription is widely applied in recent years, because of limited sources and rising price, the adulteration and counterfeiting of safflower are serious, and illegal personnel often dye and disguise the safflower or dye inferior products as superior products. The addition of the coloring agent seriously affects the curative effect and is harmful to human health. For example, acid red 73, also known as acid scarlet GR, is a yellow-light red powder, a food-prohibited pigment, and carcinogenic; carmine is a water-soluble synthetic pigment, is very easy to damage liver, and can cause cancer after being accumulated in human body for a long time.
The identification method of the false-doping dyeing of safflower has more literature reports, but the dyeing in Chinese patent medicine has few reports.
Disclosure of Invention
The invention aims to provide a method for simultaneously screening 8 illegal coloring agents in a tablet for relaxing muscles and stimulating blood circulation, aiming at the existing problems.
The invention is realized by the following technical scheme:
a method for simultaneously screening 8 illegal coloring agents in a tablet for relaxing muscles and stimulating blood circulation comprises the following steps:
(1) solution preparation:
preparation of control reagent solution:
taking a proper amount of sunset yellow contrast reagent, lemon yellow contrast reagent, acid red 73 contrast reagent, carmine contrast reagent, azorubine contrast reagent, erythrosine contrast reagent, golden orange II contrast reagent and allure red contrast reagent, and adding 70% ethanol to prepare contrast reagent solution;
preparing a test solution:
taking 20 tablets of the tendon-relaxing and blood-activating tablet, removing the coating of the coated tablet, grinding, precisely weighing 2g, placing in a conical flask with a plug, adding 20mL of 70% ethanol, carrying out ultrasonic treatment for 20min, filtering, recovering the solvent from the filtrate, placing in a 5mL measuring flask, adding 70% ethanol to the scale, and shaking uniformly to obtain a sample solution;
negative solution:
the sample solution without the dyeing reagent is negative sample solution;
(2) chromatographic conditions are as follows:
a chromatographic column: agilent ZORBAX SB-C18 column (4.6 mm. times.250 mm, 5 μm);
mobile phase: acetonitrile (A) -0.05 mol. L-1Ammonium acetate solution (B) is eluted according to a gradient (0-23 min, 10% → 48.5% A; 23-28 min, 10% A);
detection wavelength: 431nm (0-3.3 min for measuring lemon yellow), 515nm (3.3-5.5 min for measuring carmine, 9.0-15.0 min for measuring allura red and azorubine, 16.1-17.5 min for measuring acid red 73), 486nm (5.5-9.0 min for measuring sunset yellow, 17.5-28.0 min for measuring golden orange II), 533nm (15.0-16.1 min for measuring erythrosine);
flow rate: 0.8 mL/min-1(ii) a Column temperature: 30 ℃;
(3) and (3) determination:
injecting 10 μ L of test solution, 2 μ L of contrast reagent solution and 10 μ L of negative solution into a liquid chromatograph for determination;
measured by HPLC-DAD method and confirmed by HPLC-MS/MS method.
Further, the power of the ultrasound in the step (1) is 300W, and the frequency is 40 kHz.
Compared with the prior art, the invention has the following advantages:
1. the application adopts an HPLC-DAD method to switch the wavelength in a gradient manner and simultaneously determine 8 coloring agents in the muscle-relaxing and blood-circulation-promoting tablets, thereby providing scientific basis for safety investigation of the muscle-relaxing and blood-circulation-promoting tablets.
2. The application adopts an HPLC-DAD method to detect 8 coloring agents in the tendon-relaxing and blood-circulation-promoting tablets, the established method inspects 46 batches of tendon-relaxing and blood-circulation-promoting tablet samples, wherein 4 batches of acid red 73 are detected, which indicates that the use condition of chemical coloring agents in Chinese patent medicines is still severe, and the attention to illegal dyeing conditions in Chinese medicinal materials and Chinese medicinal decoction pieces and the supervision strength should be increased so as to ensure the safety and effectiveness of the medicines.
3. The application has the greatest advantages that 8 illegal coloring agents in the muscle-relaxing and blood-circulation-promoting tablets are screened simultaneously by an HPLC-DAD method; the method adopts an HPLC-DAD method for determination, and adopts an HPLC-MS/MS method to confirm the screening of illegal coloring agents in the Chinese medicinal tablet for relaxing muscles and tendons and promoting blood circulation.
Drawings
FIG. 1 is a liquid chromatogram mixed control reagent (A), a tendon-relaxing and blood-circulation-promoting tablet positive sample (B) and a tendon-relaxing and blood-circulation-promoting tablet negative sample (C);
FIG. 2 is a spectrum (A) of acid red 73 and a spectrum (B) of a positive sample of Shujinhuoxue tablet;
fig. 3 is a mass spectrum of acid red 73(a), tendon-relaxing and blood-activating tablet positive sample (B) and tendon-relaxing and blood-activating tablet negative sample (C).
Detailed Description
A method for simultaneously screening 8 illegal coloring agents in a tablet for relaxing muscles and stimulating blood circulation comprises the following steps:
(1) solution preparation:
preparation of control reagent solution:
taking a proper amount of sunset yellow contrast reagent, lemon yellow contrast reagent, acid red 73 contrast reagent, carmine contrast reagent, azorubine contrast reagent, erythrosine contrast reagent, golden orange II contrast reagent and allure red contrast reagent, and adding 70% ethanol to prepare contrast reagent solution;
preparing a test solution:
taking 20 tablets of the tendon-relaxing and blood-activating tablet, removing the coating of the coated tablet, grinding, precisely weighing 2g, placing in a conical flask with a plug, adding 20mL of 70% ethanol, carrying out ultrasonic treatment for 20min, filtering, recovering the solvent from the filtrate, placing in a 5mL measuring flask, adding 70% ethanol to the scale, and shaking uniformly to obtain a sample solution;
negative solution:
the sample solution without the dyeing reagent is negative sample solution;
(2) chromatographic conditions are as follows:
a chromatographic column: agilent ZORBAX SB-C18 column (4.6 mm. times.250 mm, 5 μm);
mobile phase: acetonitrile (A) -0.05 mol. L-1Ammonium acetate solution (B) is eluted according to a gradient (0-23 min, 10% → 48.5% A; 23-28 min, 10% A);
detection wavelength: 431nm (0-3.3 min for measuring lemon yellow), 515nm (3.3-5.5 min for measuring carmine, 9.0-15.0 min for measuring allura red and azorubine, 16.1-17.5 min for measuring acid red 73), 486nm (5.5-9.0 min for measuring sunset yellow, 17.5-28.0 min for measuring golden orange II), 533nm (15.0-16.1 min for measuring erythrosine);
flow rate: 0.8 mL/min-1(ii) a Column temperature: 30 ℃;
(3) and (3) determination:
injecting 10 μ L of test solution, 2 μ L of contrast reagent solution and 10 μ L of negative solution into a liquid chromatograph for determination;
measured by HPLC-DAD method and confirmed by HPLC-MS/MS method.
The power of the ultrasound in the step (1) is 300W, and the frequency is 40 kHz.
In FIG. 1, 1 is lemon yellow (Tartrazine)2 is Carmine (Carmine)3 is Sunset yellow (Sunset yellow) 4 is allura red (Allure)5 is azorubine (Azorubin)6 is erythrosine (Pyrrosine) 7 is Acid red 73(Acid red 73)8 is Gold orange II (Gold orange II).
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the present invention is not limited to the illustrated embodiments, and all the modifications and equivalents of the embodiments may be made without departing from the spirit of the present invention.
Claims (2)
1. A method for simultaneously screening 8 illegal coloring agents in a tablet for relaxing muscles and stimulating blood circulation is characterized by comprising the following steps:
(1) solution preparation:
preparation of control reagent solution:
taking a proper amount of sunset yellow contrast reagent, lemon yellow contrast reagent, acid red 73 contrast reagent, carmine contrast reagent, azorubine contrast reagent, erythrosine contrast reagent, golden orange II contrast reagent and allure red contrast reagent, and adding 70% ethanol to prepare contrast reagent solution;
preparing a test solution:
taking 20 tablets of the tendon-relaxing and blood-activating tablet, removing the coating of the coated tablet, grinding, precisely weighing 2g, placing in a conical flask with a plug, adding 20mL of 70% ethanol, carrying out ultrasonic treatment for 20min, filtering, recovering the solvent from the filtrate, placing in a 5mL measuring flask, adding 70% ethanol to the scale, and shaking uniformly to obtain a sample solution;
negative solution:
the sample solution without the dyeing reagent is negative sample solution;
(2) chromatographic conditions are as follows:
a chromatographic column: agilent ZORBAX SB-C18 column (4.6 mm. times.250 mm, 5 μm);
mobile phase: acetonitrile (A) -0.05 mol. L-1An ammonium acetate solution (B) is eluted according to a gradient (0-23 min, 10% → 48.5% A; 23-28 min, 10% A);
detection wavelength: 431nm (0-3.3 min for measuring lemon yellow), 515nm (3.3-5.5 min for measuring carmine, 9.0-15.0 min for measuring allura red and azorubine, 16.1-17.5 min for measuring acid red 73), 486nm (5.5-9.0 min for measuring sunset yellow, 17.5-28.0 min for measuring golden orange II), 533nm (15.0-16.1 min for measuring erythrosine);
flow rate: 0.8 mL/min-1(ii) a Column temperature: 30 ℃;
(3) and (3) determination:
injecting 10 μ L of test solution, 2 μ L of contrast reagent solution and 10 μ L of negative solution into a liquid chromatograph for determination;
measured by HPLC-DAD method and confirmed by HPLC-MS/MS method.
2. The method for simultaneously screening 8 illegal staining agents in the tablet for relaxing muscles and stimulating blood circulation according to claim 1, wherein the power of the ultrasound in step (1) is 300W, and the frequency is 40 kHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111350123.4A CN114062546A (en) | 2021-11-15 | 2021-11-15 | Method for simultaneously screening 8 illegal coloring agents in muscle-relaxing and blood-circulation-promoting tablets |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111350123.4A CN114062546A (en) | 2021-11-15 | 2021-11-15 | Method for simultaneously screening 8 illegal coloring agents in muscle-relaxing and blood-circulation-promoting tablets |
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| CN114062546A true CN114062546A (en) | 2022-02-18 |
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| CN202111350123.4A Pending CN114062546A (en) | 2021-11-15 | 2021-11-15 | Method for simultaneously screening 8 illegal coloring agents in muscle-relaxing and blood-circulation-promoting tablets |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007006171A1 (en) * | 2005-07-07 | 2007-01-18 | Guangzhou Perfect Set Medicine Limited | A traditional chinese medicine composition, the preparation and quality controlling method thereof |
| CN102552616A (en) * | 2010-12-29 | 2012-07-11 | 江西济民可信集团有限公司 | Detection method for tablets capable of relaxing muscles and stimulating blood circulation |
| CN113358785A (en) * | 2021-06-04 | 2021-09-07 | 武汉药品医疗器械检验所 | Method for detecting illegal staining pigments of 18 traditional Chinese medicines based on HPLC-DAD combined UPLC-MS/MS method |
-
2021
- 2021-11-15 CN CN202111350123.4A patent/CN114062546A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007006171A1 (en) * | 2005-07-07 | 2007-01-18 | Guangzhou Perfect Set Medicine Limited | A traditional chinese medicine composition, the preparation and quality controlling method thereof |
| CN102552616A (en) * | 2010-12-29 | 2012-07-11 | 江西济民可信集团有限公司 | Detection method for tablets capable of relaxing muscles and stimulating blood circulation |
| CN113358785A (en) * | 2021-06-04 | 2021-09-07 | 武汉药品医疗器械检验所 | Method for detecting illegal staining pigments of 18 traditional Chinese medicines based on HPLC-DAD combined UPLC-MS/MS method |
Non-Patent Citations (2)
| Title |
|---|
| 李启艳;权洪远;姜国萍;谢强胜;朱日然;: "中药中非法添加色素的薄层色谱快速鉴别法研究", 药学研究, vol. 36, no. 01, pages 21 - 24 * |
| 许广英;王绚;戴希希;陈敏;: "舒筋活血丸的质量标准研究", 中成药, vol. 30, no. 11, pages 1638 - 1642 * |
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