CN114058483A - Purification equipment for umbilical cord mesenchymal stem cells and operation method thereof - Google Patents

Purification equipment for umbilical cord mesenchymal stem cells and operation method thereof Download PDF

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Publication number
CN114058483A
CN114058483A CN202111426837.9A CN202111426837A CN114058483A CN 114058483 A CN114058483 A CN 114058483A CN 202111426837 A CN202111426837 A CN 202111426837A CN 114058483 A CN114058483 A CN 114058483A
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mesenchymal stem
umbilical cord
cord mesenchymal
stem cells
purification
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张扬
徐健
李陶
周晓宇
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Zhongkebaocheng Biomedical Technology Co ltd
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Zhongkebaocheng Biomedical Technology Co ltd
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Abstract

The invention discloses a purification device of umbilical cord mesenchymal stem cells, which comprises a box body, a motor I, a purification barrel, a motor II, a rotating gear and a planetary gear, and is characterized in that: the box setting on the support, motor one set up in the bottom half, purification bucket bottom be provided with row material pipe, motor two set up at the box top, rotary gear set up in two bottoms of transmission shaft, planetary gear place between solid fixed ring and rotary gear, be connected planetary gear and solid fixed ring, rotary gear to be provided with the dead lever on planetary gear. According to the invention, the rotating gear drives the planetary gear and the fixed rods on the planetary gear to rotate, so that the fixed rods move in the purification barrel between the chromatography layer and the molecular sieve layer, the plurality of planetary gears drive the plurality of fixed rods to stir the umbilical cord mesenchymal stem cells in the purification barrel, the umbilical cord mesenchymal stem cells are fully filtered and purified, and the purification quality of the umbilical cord mesenchymal stem cells is improved.

Description

Purification equipment for umbilical cord mesenchymal stem cells and operation method thereof
Technical Field
The invention relates to the technical field of umbilical cord mesenchymal stem cell production, in particular to purification equipment of umbilical cord mesenchymal stem cells and an operation method thereof.
Background
Umbilical cord mesenchymal stem cells are used as reagents for in vitro diagnosis, medicines for treating diseases, ligands for immunoaffinity chromatography and the like, the umbilical cord mesenchymal stem cells are used as core reagents for various immunoassays, play an important role in the sensitivity and specificity of immunoassay results, the umbilical cord mesenchymal stem cells need to be purified in the preparation process, and the existing umbilical cord mesenchymal stem cell purification device has the problems of low purification efficiency, easy cross infection in the purification process and poor purification quality.
Disclosure of Invention
The invention aims to solve the problems of low purification efficiency, easy cross infection in the purification process and poor purification quality of the conventional umbilical cord mesenchymal stem cell purification device, and provides purification equipment for umbilical cord mesenchymal stem cells and an operation method thereof, wherein the purification equipment is reasonable in structural design, high in working efficiency, difficult to infect in the umbilical cord mesenchymal stem cell purification process and high in umbilical cord mesenchymal stem cell purification quality.
The technical scheme adopted for solving the technical problem is as follows:
the utility model provides a purification equipment of umbilical cord mesenchymal stem cell, includes box, motor one, purification bucket, motor two, rotating gear and planetary gear, its characterized in that: the purifying barrel is characterized in that the box body is arranged on a support, the top of the box body is provided with a feeding pipe, the bottom of the box body is provided with a bearing I, the top of the box body is provided with a fixed box, the bottom of the fixed box is provided with a fixed ring, a bearing II and a blanking pipe, the motor I is arranged at the bottom of the box body, the motor I is provided with a transmission shaft I, the transmission shaft I vertically extends into the box body, the transmission shaft I is provided with a driving wheel, the bottom of the purifying barrel is provided with a discharging pipe, the discharging pipe vertically penetrates out of the bearing I, a valve is arranged on the discharging pipe, the motor II is arranged at the top of the box body, the motor II is provided with a transmission shaft II, the transmission shaft II vertically penetrates through the bearing II, the rotating gear is arranged at the bottom end of the transmission shaft II, the planetary gear is arranged between the fixed ring and the rotating gear, and is connected with the fixed ring and the rotating gear, and a fixed rod is arranged on the planetary gear, umbilical cord mesenchymal stem cells to be purified are added into a fixed box from a feeding pipe, after the umbilical cord mesenchymal stem cells are added, a sealing cover covers the feeding pipe, the umbilical cord mesenchymal stem cells in the fixed box flow into a purification barrel between the inner wall of the purification barrel and a chromatographic layer from a blanking pipe under the action of gravity, a first motor drives a first transmission shaft and a driving wheel on the first transmission shaft to rotate, the driving wheel drives a driven wheel on a discharging pipe to rotate, so that the purification barrel and the umbilical cord mesenchymal stem cells in the purification barrel are driven to rotate, the umbilical cord mesenchymal stem cells on one side of the chromatographic layer flow to the middle part of the purification barrel through the chromatographic layer and a molecular sieve layer, the umbilical cord mesenchymal stem cells are filtered and purified, a second motor drives a second transmission shaft and a rotary gear on the second transmission shaft to rotate, and the rotary gear drives the planetary gear and the fixed rod on the planetary gear to rotate, make the dead lever move in the purification bucket between chromatography layer and molecular sieve layer, stir the umbilical cord mesenchymal stem cell between chromatography layer and the molecular sieve layer, it flows to discharge the intraductal flow to passing the umbilical cord mesenchymal stem cell on molecular sieve layer, through the chromatography layer, the molecular sieve layer is to umbilical cord mesenchymal stem cell screening purification, the purification quality of umbilical cord mesenchymal stem cell has been improved, in order to facilitate the washing of purification equipment, can divide into two sections structures with the box, make the box top can drive the dead lever and rise to shift out or stretch into the purification bucket with the dead lever in following the purification bucket, the realization is to the combination or the partition of purification equipment, be convenient for to clearing up in the purification bucket and handle.
Furthermore, a booster pump is arranged at the top of the box body, a gas pipe is arranged on the booster pump, one end of the gas pipe extends into the fixed box, the booster pump blows bacteria-free gas flow into the fixed box through the gas pipe, the sterile gas flow enters the purification barrel between the inner wall of the purification barrel and the chromatographic layer through the blanking pipe, the pressure of the region is increased, umbilical cord mesenchymal stem cells in the region flow to the region between the chromatographic layer and the molecular sieve layer through the chromatographic layer and flow to the region formed by the molecular sieve layer through the molecular sieve layer, the purification of the umbilical cord mesenchymal stem cells is accelerated, the gas pressure of each region in the box body tends to be the same along with the increase of input gas flow, the purification rate of the umbilical cord mesenchymal stem cells tends to be gentle, after the purification of the umbilical cord mesenchymal stem cells is finished, the gas pressure in the box body is high, and the purified umbilical cord mesenchymal stem cells in the region formed by the molecular sieve layer are rapidly discharged from the discharge pipe, and the purified umbilical cord mesenchymal stem cells in other areas rapidly flow to the area consisting of the molecular sieve under the action of airflow pressure, and are the umbilicus again.
Further, the box inner wall on be provided with the locating plate, be provided with the connecting axle between locating plate and locating plate, be provided with the auxiliary wheel on the connecting axle between locating plate and locating plate, and with auxiliary wheel and purification bucket outer wall connection, the first action wheel that drives transmission shaft one and transmission shaft one is rotatory, the action wheel drives to arrange the follow driving wheel rotation on the material pipe, thereby it is rotatory to drive umbilical cord mesenchymal stem cell in purification bucket and the purification bucket, the umbilical cord mesenchymal stem cell that makes chromatography layer one side passes through the chromatography layer, the molecular sieve layer flows to purification bucket middle part, filter the purification to umbilical cord mesenchymal stem cell, can carry on spacingly to the purification bucket of rotation in-process through the auxiliary wheel, improve the stability of purification bucket in rotation process, and then improve the stability of purification bucket in the course of the work
Further, the row in the box be provided with on the material pipe from the driving wheel to will follow the driving wheel on driving shaft one and be connected, the first action wheel that drives transmission shaft one and transmission shaft one is rotatory, the driving wheel drives to arrange the rotation of driving wheel on the material pipe, thereby it is rotatory to drive the umbilical cord mesenchymal stem cell in purification bucket and the purification bucket, makes the umbilical cord mesenchymal stem cell of chromatography layer one side flow to purification bucket middle part through chromatography layer, molecular sieve layer, filters the purification to umbilical cord mesenchymal stem cell.
Further, a chromatography layer and a molecular sieve layer are arranged in the purification barrel, the bottom end of the blanking pipe is extended into the purification barrel between the inner wall of the purification barrel and the chromatography layer, umbilical cord mesenchymal stem cells to be purified are added into the fixed box from the feeding pipe, the feeding pipe is covered with the sealing cover after the umbilical cord mesenchymal stem cells are added, the umbilical cord mesenchymal stem cells in the fixed box flow into the purification barrel between the inner wall of the purification barrel and the chromatography layer from the blanking pipe under the action of gravity, the first motor drives the first transmission shaft and the driving wheel on the first transmission shaft to rotate, the driving wheel drives the driven wheel on the discharge pipe to rotate, thereby driving the purification barrel and the umbilical cord mesenchymal stem cells in the purification barrel to rotate, the umbilical cord mesenchymal stem cells on one side of the chromatography layer flow to the middle part of the purification barrel through the chromatography layer and the molecular sieve layer, the umbilical cord mesenchymal stem cells are filtered and purified, and the second motor drives the rotating gear on the second transmission shaft to rotate, the rotating gear drives the planetary gear and the fixing rod on the planetary gear to rotate, so that the fixing rod moves in the purification barrel between the chromatography layer and the molecular sieve layer, umbilical cord mesenchymal stem cells between the chromatography layer and the molecular sieve layer are stirred, the umbilical cord mesenchymal stem cells flow into the discharge pipe through the molecular sieve layer, and the umbilical cord mesenchymal stem cells are screened and purified through the chromatography layer and the molecular sieve layer, so that the purification quality of the umbilical cord mesenchymal stem cells is improved.
Furthermore, the number of the planetary gears is 2-6, the bottom ends of the fixed rods on the planetary gears extend into the purification barrel between the chromatography layer and the molecular sieve layer, the second motor drives the second transmission shaft and the rotary gears on the second transmission shaft to rotate, the rotary gears drive the planetary gears and the fixed rods on the planetary gears to rotate, so that the fixed rods move in the purification barrel between the chromatography layer and the molecular sieve layer, the plurality of planetary gears drive the plurality of fixed rods to stir the umbilical cord mesenchymal stem cells in the purification barrel, the umbilical cord mesenchymal stem cells are fully filtered and purified, and the purification quality of the umbilical cord mesenchymal stem cells is improved.
Further, motor one set up to syntropy or reverse rotation's structure with motor two, motor one provides power for the rotation of purification bucket, motor two provides power for planetary gear's rotation, with purification bucket and planetary gear syntropy or reverse rotation, can make the dead lever on the planetary gear present irregular motion in the purification bucket, carry out abundant stirring to the umbilical cord mesenchymal stem cell in the purification bucket and handle, carry out abundant purification to umbilical cord mesenchymal stem cell and handle, strengthen the purification quality of umbilical cord mesenchymal stem cell.
Further, the box on the inlet pipe top be provided with sealed lid, through threaded connection between sealed lid and the inlet pipe, open sealed lid, through the inlet pipe to injecting umbilical cord mesenchymal stem cell into in the fixed case, after injecting umbilical cord mesenchymal stem cell, be connected sealed lid and inlet pipe, improve the leakproofness of purification equipment, avoid receiving the pollution to umbilical cord mesenchymal stem cell among the purification process, improved umbilical cord mesenchymal stem cell's purification effect.
An operation method of a purification device suitable for umbilical cord mesenchymal stem cells is characterized in that: the operation method comprises the following steps:
firstly, the method comprises the following steps: transferring the purification equipment of the umbilical cord mesenchymal stem cells to a proper position, and checking whether the line of the purification equipment of the umbilical cord mesenchymal stem cells is normal or not, and whether the motor I, the motor II and the booster pump can work normally or not;
secondly, the method comprises the following steps: connecting the motor I, the motor II and the booster pump with an external control system, and connecting the control system with a power supply;
thirdly, the method comprises the following steps: closing a valve on the discharging pipe, opening a sealing cover on the feeding pipe, adding the umbilical cord mesenchymal stem cells to be purified into the fixing box from the feeding pipe, covering the feeding pipe with the sealing cover after the umbilical cord mesenchymal stem cells are added, and allowing the umbilical cord mesenchymal stem cells in the fixing box to flow into the purifying barrel between the inner wall of the purifying barrel and the chromatographic layer from the blanking pipe under the action of gravity;
fourthly: starting a motor I and a motor II, wherein the motor I drives a transmission shaft I and a driving wheel on the transmission shaft I to rotate, the driving wheel drives a driven wheel on a discharge pipe to rotate, so that the purification barrel and the umbilical cord mesenchymal stem cells in the purification barrel are driven to rotate, the umbilical cord mesenchymal stem cells on one side of a chromatography layer flow to the middle part of the purification barrel through the chromatography layer and a molecular sieve layer, the umbilical cord mesenchymal stem cells are filtered and purified, the motor II drives a transmission shaft II and a rotating gear on the transmission shaft II to rotate, the rotating gear drives a planetary gear and a fixed rod on the planetary gear to rotate, the fixed rod moves in the purification barrel between the chromatography layer and the molecular sieve layer, the umbilical cord mesenchymal stem cells between the chromatography layer and the molecular sieve layer are stirred, and the umbilical cord mesenchymal stem cells flow to the discharge pipe through the molecular sieve layer;
fifth, the method comprises the following steps: starting a booster pump, wherein the booster pump blows sterile air flow into the fixed box through a gas pipe to enhance the air pressure in the fixed box and the box body, so that the umbilical cord mesenchymal stem cells in the purification barrel can flow from the edge of the inner wall of the purification barrel to the middle part;
sixth: after the concentration of the umbilical cord mesenchymal stem cells is finished, continuously blowing sterile air flow into the fixed box, opening a valve on a discharge pipe, discharging the purified umbilical cord mesenchymal stem cells in the middle of the purification barrel from the discharge pipe, after the discharge of the umbilical cord mesenchymal stem cells is finished, closing a booster pump, a motor I and a motor II, cleaning impurities generated in the purification process of the umbilical cord mesenchymal stem cells, cleaning purification equipment of the umbilical cord mesenchymal stem cells, and cutting off a power supply.
Has the advantages that: the invention adds umbilical cord mesenchymal stem cells to be purified into a fixed box from a feeding pipe, the sealed cover covers the feeding pipe after the umbilical cord mesenchymal stem cells are added, the umbilical cord mesenchymal stem cells in the fixed box flow into a purifying barrel between the inner wall of the purifying barrel and a chromatographic layer from a blanking pipe under the action of gravity, a motor I drives a transmission shaft I and a driving wheel on the transmission shaft I to rotate, the driving wheel drives a driven wheel on a discharging pipe to rotate, so that the purifying barrel and the umbilical cord mesenchymal stem cells in the purifying barrel are driven to rotate, the umbilical cord mesenchymal stem cells on one side of the chromatographic layer flow to the middle part of the purifying barrel through the chromatographic layer and a molecular sieve layer, the umbilical cord mesenchymal stem cells are filtered and purified, a motor II drives a transmission shaft II and a rotating gear on the transmission shaft II to rotate, the rotating gear drives a planetary gear and a fixed rod on the planetary gear to rotate, so that the fixed rod moves in the purifying barrel between the chromatographic layer and the molecular sieve layer, agitating the umbilical cord mesenchymal stem cells between the chromatography layer and the molecular sieve layer, enabling the umbilical cord mesenchymal stem cells to flow into the discharge pipe through the molecular sieve layer, and screening and purifying the umbilical cord mesenchymal stem cells through the chromatography layer and the molecular sieve layer, so that the purification quality of the umbilical cord mesenchymal stem cells is improved.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a partial structural schematic view of the present invention, illustrating a connection structure of the case and the auxiliary wheel.
FIG. 3 is a schematic view of a part of the structure of the present invention, showing the connection structure of the purification cartridge and the chromatographic layer.
Fig. 4 is a partial structural view of the present invention, illustrating a connection structure of a rotary gear and a planetary gear.
Fig. 5 is a schematic diagram of another embodiment of the present invention.
In the figure: 1. the device comprises a box body, a first motor, a second motor, a third motor, a purifying barrel, a fourth motor, a fifth motor, a sixth motor, a fifth motor, a sixth motor, a fifth, a sixth motor, a fifth motor, a sixth motor, a fifth, a sixth motor, a fifth, a sixth motor, a fifth, a sixth motor, a fifth, a sixth, a fifth, a sixth, a fifth, a sixth, a fifth, a sixth, a fifth, a sixth, a fifth, a sixth, a fifth, a sixth.
Detailed Description
The invention will be described in more detail below with reference to the accompanying drawings.
The first embodiment is as follows:
as shown in fig. 1-4, a purification equipment of umbilical cord mesenchymal stem cells, includes box 1, motor one 2, purification bucket 3, motor two 4, rotating gear 5 and planetary gear 6, its characterized in that: the purification device is characterized in that the box body 1 is arranged on a support 7, the top of the box body 1 is provided with a feeding pipe 8, the bottom of the box body 1 is provided with a first bearing 10, the top of the inside of the box body 1 is provided with a fixed box 9, the bottom of the fixed box 9 is provided with a fixed ring 12, a second bearing 11 and a blanking pipe 13, the first motor 2 is arranged at the bottom of the box body 1, the first motor 2 is provided with a first transmission shaft 20, the first transmission shaft 20 vertically extends into the box body 1 and is provided with a driving wheel 21, the bottom of the first transmission shaft 20 is provided with a discharge pipe 22, the discharge pipe 22 vertically penetrates out of the first bearing 10 and is provided with a valve 26, the second motor 4 is arranged at the top of the box body 1, the second transmission shaft 27 is arranged on the second motor 4 and vertically penetrates through the second bearing 11, the rotary gear 5 is arranged at the bottom end of the second transmission shaft 27, the device comprises a planetary gear 6, a fixed ring 12, a rotating gear 5, a fixed rod 28, a fixed box 9, a sealing cover 17, a feeding pipe 8, a purification barrel 3, a motor I2, a transmission shaft I20, a driving wheel 21 on the transmission shaft I20, a discharging pipe 22, a driven wheel 25 on the purification barrel 3 and the purification barrel 3, wherein the planetary gear 6 is arranged between the fixed ring 12 and the rotating gear 5, the planetary gear 6 is connected with the fixed ring 12 and the rotating gear 5, the fixed rod 28 is arranged on the planetary gear 6, umbilical cord mesenchymal stem cells to be purified are fed into the fixed box 9 from the feeding pipe 8, the umbilical cord mesenchymal stem cells are fed into the purification barrel 3 between the inner wall of the purification barrel 3 and a chromatographic layer 23, the sealing cover 17 covers the feeding pipe 8, the umbilical cord mesenchymal stem cells in the fixed box 9 flow into the purification barrel 3 from a discharging pipe 13 under the action of gravity, the motor I2 drives the transmission shaft I20 and the driving wheel 21 on the transmission shaft I20 to rotate, the driving wheel 21 drives the driven wheel 25 on the discharging pipe 22 to rotate, thereby driving the umbilical cord mesenchymal stem cells in the purification barrel 3 and the purification barrel 3 to rotate, the umbilical cord mesenchymal stem cells on one side of the purification barrel 3 to flow to the middle of the chromatographic layer 23 and the molecular sieve layer 24 to the purification barrel 3, the umbilical cord mesenchymal stem cells are filtered and purified, the motor II 4 drives the transmission shaft II 27 and the rotating gear 5 on the transmission shaft II 27 to rotate, the rotating gear 5 drives the planetary gear 6 and the fixing rod 28 on the planetary gear 6 to rotate, the fixing rod 28 moves in the purification barrel 3 between the chromatographic layer 23 and the molecular sieve layer 24, the umbilical cord mesenchymal stem cells between the chromatographic layer 23 and the molecular sieve layer 24 are stirred, the umbilical cord mesenchymal stem cells flow into the discharge pipe 22 through the molecular sieve layer 24, the umbilical cord mesenchymal stem cells are screened and purified through the chromatographic layer 23 and the molecular sieve layer 24, the purification quality of the umbilical cord mesenchymal stem cells is improved, in order to facilitate the cleaning of the purification equipment, the box body 1 can be divided into two sections, the top of the box body 1 can drive the fixing rod 28 to ascend and move out of the purification barrel 3 or stretch the fixing rod 28 into the purification barrel 3, the combination or the division of the purification equipment is realized, is convenient for cleaning the purification barrel 3.
Further, a booster pump 18 is arranged at the top of the box body 1, a gas pipe 19 is arranged on the booster pump 18, one end of the gas pipe 19 extends into the fixed box 9, the booster pump 18 blows bacteria-free gas flow into the fixed box 9 through the gas pipe 19, the sterile gas flow enters the purifying barrel 3 between the inner wall of the purifying barrel 3 and the chromatographic layer 23 through the blanking pipe 13, the pressure of the region is increased, umbilical cord mesenchymal stem cells in the region flow to the region between the chromatographic layer 23 and the molecular sieve layer 24 through the chromatographic layer 23 and flow to the region formed by the molecular sieve layer 24 through the molecular sieve layer 24, the purification of the umbilical cord mesenchymal stem cells is accelerated, the gas pressure of each region in the box body 1 tends to be the same along with the increase of input gas flow, the purification rate of the umbilical cord mesenchymal stem cells tends to be flat, after the purification of the umbilical cord mesenchymal stem cells is completed, the gas pressure in the box body 1 is large, the purified umbilical cord mesenchymal stem cells in the area formed by the molecular sieve layer 24 are quickly discharged from the discharge pipe 22, and the purified umbilical cord mesenchymal stem cells in other areas quickly flow into the area formed by the molecular sieve under the action of airflow pressure to form the umbilicus again.
Furthermore, a positioning plate 14 is arranged on the inner wall of the box body 1, a connecting shaft 15 is arranged between the positioning plate 14 and the positioning plate 14, an auxiliary wheel 16 is arranged on a connecting shaft 15 between the positioning plate 14 and the positioning plate 14, the auxiliary wheel 16 is connected with the outer wall of the purification barrel 3, a motor I2 drives a transmission shaft I20 and a driving wheel 21 on the transmission shaft I20 to rotate, the driving wheel 21 drives a driven wheel 25 on a discharge pipe 22 to rotate, thereby driving the purification barrel 3 and the umbilical cord mesenchymal stem cells in the purification barrel 3 to rotate, leading the umbilical cord mesenchymal stem cells at one side of the chromatographic layer 23 to flow to the middle part of the purification barrel 3 through the chromatographic layer 23 and the molecular sieve layer 24, carry out the filtration purification to umbilical cord mesenchymal stem cell, can carry out spacingly through auxiliary wheel 16 to the purification bucket 3 of rotation in-process, improve the stability of purification bucket 3 in rotation process, and then improve the stability of purification bucket 3 in the course of the work.
Further, the row in box 1 arrange and be provided with on the material pipe 22 from the driving wheel 25 to will be connected from driving wheel 25 and the action wheel 21 on the transmission shaft 20, the action wheel 21 that motor 2 drove transmission shaft 20 and transmission shaft 20 is rotatory, action wheel 21 drives row and arranges the rotation of driving wheel 25 on the material pipe 22, thereby it is rotatory to drive umbilical cord mesenchymal stem cell in purification bucket 3 and the purification bucket 3, makes the umbilical cord mesenchymal stem cell of chromatography layer 23 one side flow to purification bucket 3 middle parts through chromatography layer 23, molecular sieve layer 24, filters the purification to umbilical cord mesenchymal stem cell.
Further, a chromatography layer 23 and a molecular sieve layer 24 are arranged in the purification barrel 3, the bottom end of the blanking pipe 13 extends into the purification barrel 3 between the inner wall of the purification barrel 3 and the chromatography layer 23, umbilical cord mesenchymal stem cells to be purified are added into the fixed box 9 from the feeding pipe 8, the sealing cover 17 covers the feeding pipe 8 after the umbilical cord mesenchymal stem cells are added, the umbilical cord mesenchymal stem cells in the fixed box 9 flow into the purification barrel 3 between the inner wall of the purification barrel 3 and the chromatography layer 23 from the blanking pipe 13 under the action of gravity, the motor I2 drives the transmission shaft I20 and the driving wheel 21 on the transmission shaft I20 to rotate, the driving wheel 21 drives the driven wheel 25 on the discharging pipe 22 to rotate, so that the purification barrel 3 and the umbilical cord mesenchymal stem cells in the purification barrel 3 are driven to rotate, the umbilical cord mesenchymal stem cells on one side of the chromatography layer 23 flow to the middle of the purification barrel 3 through the chromatography layer 23 and the molecular sieve layer 24, the umbilical cord mesenchymal stem cells are filtered and purified, the motor II 4 drives the transmission shaft II 27 and the rotating gear 5 on the transmission shaft II 27 to rotate, the rotating gear 5 drives the planetary gear 6 and the fixing rod 28 on the planetary gear 6 to rotate, the fixing rod 28 moves in the purification barrel 3 between the chromatography layer 23 and the molecular sieve layer 24, the umbilical cord mesenchymal stem cells between the chromatography layer 23 and the molecular sieve layer 24 are stirred, the umbilical cord mesenchymal stem cells flow into the discharge pipe 22 through the molecular sieve layer 24, and the umbilical cord mesenchymal stem cells are screened and purified through the chromatography layer 23 and the molecular sieve layer 24, so that the purification quality of the umbilical cord mesenchymal stem cells is improved.
Further, planetary gear 6, its quantity is 4 to stretch into purification bucket 3 between chromatography layer 23 and molecular sieve layer 24 with dead lever 28 bottom on the planetary gear 6, motor two 4 drive transmission shaft two 27 and the rotatory gear 5 on the transmission shaft two 27 is rotatory, rotatory gear 5 drives planetary gear 6 and the dead lever 28 on the planetary gear 6 rotatory, make dead lever 28 move in purification bucket 3 between chromatography layer 23 and molecular sieve layer 24, a plurality of planetary gear 6 drive many dead levers 28 stir umbilical cord mesenchymal stem cell in purification bucket 3, carry out abundant filtration purification to umbilical cord mesenchymal stem cell, improve umbilical cord mesenchymal stem cell's purification quality.
Further, motor 2 and motor two 4 set up to syntropy or reverse rotation's structure, motor 2 provides power for the rotation of purification bucket 3, motor two 4 provides power for planetary gear 6's rotation, with purification bucket 3 and planetary gear 6 syntropy or reverse rotation, can make dead lever 28 on the planetary gear 6 present irregular motion in purification bucket 3, carry out abundant stirring to the umbilical cord mesenchymal stem cell in the purification bucket 3 and handle, carry out abundant purification to umbilical cord mesenchymal stem cell, the purification quality of reinforcing umbilical cord mesenchymal stem cell.
Further, 8 tops of inlet pipe on box 1 be provided with sealed lid 17, through threaded connection between sealed lid 17 and the inlet pipe 8, open sealed lid 17, inject umbilical cord mesenchymal stem cell into through inlet pipe 8 in to fixed case 9, after injecting into umbilical cord mesenchymal stem cell, be connected sealed lid 17 and inlet pipe 8, improve the leakproofness of purification equipment, avoid receiving the pollution to the umbilical cord mesenchymal stem cell among the purification process, improved the purification effect of umbilical cord mesenchymal stem cell.
An operation method of a purification device suitable for umbilical cord mesenchymal stem cells is characterized in that: the operation method comprises the following steps:
firstly, the method comprises the following steps: transferring the purification equipment of the umbilical cord mesenchymal stem cells to a proper position, and checking whether the line of the purification equipment of the umbilical cord mesenchymal stem cells is normal or not, and whether the motor I2, the motor II 4 and the booster pump 18 can work normally or not;
secondly, the method comprises the following steps: connecting a first motor 2, a second motor 4 and a booster pump 18 with an external control system, and connecting the control system with a power supply;
thirdly, the method comprises the following steps: closing a valve 26 on a discharging pipe 22, opening a sealing cover 17 on a feeding pipe 8, adding umbilical cord mesenchymal stem cells to be purified into a fixed box 9 from the feeding pipe 8, covering the feeding pipe 8 with the sealing cover 17 after the umbilical cord mesenchymal stem cells are added, and allowing the umbilical cord mesenchymal stem cells in the fixed box 9 to flow into the purifying barrel 3 between the inner wall of the purifying barrel 3 and the chromatographic layer 23 from a blanking pipe 13 under the action of gravity;
fourthly: starting the first motor 2 and the second motor 4, wherein the first motor 2 drives the first transmission shaft 20 and the driving wheel 21 on the first transmission shaft 20 to rotate, the driving wheel 21 drives the driven wheel 25 on the discharge pipe 22 to rotate, thereby driving the purification barrel 3 and the umbilical cord mesenchymal stem cells in the purification barrel 3 to rotate, leading the umbilical cord mesenchymal stem cells at one side of the chromatographic layer 23 to flow to the middle part of the purification barrel 3 through the chromatographic layer 23 and the molecular sieve layer 24, filtering and purifying the umbilical cord mesenchymal stem cells, driving a second transmission shaft 27 and a rotating gear 5 on the second transmission shaft 27 to rotate by a second motor 4, driving a planetary gear 6 and a fixing rod 28 on the planetary gear 6 to rotate by the rotating gear 5, enabling the fixing rod 28 to move in the purifying barrel 3 between the chromatographic layer 23 and the molecular sieve layer 24, stirring the umbilical cord mesenchymal stem cells between the chromatographic layer 23 and the molecular sieve layer 24, and enabling the umbilical cord mesenchymal stem cells to flow into the discharge pipe 22 through the molecular sieve layer 24;
fifth, the method comprises the following steps: starting the booster pump 18, wherein the booster pump 18 blows sterile air flow into the fixed box 9 through the air conveying pipe 19 to enhance the air pressure in the fixed box 9 and the box body 1, so that the umbilical cord mesenchymal stem cells in the purification barrel 3 can flow from the edge of the inner wall of the purification barrel 3 to the middle part;
sixth: after the concentration of the umbilical cord mesenchymal stem cells is finished, continuously blowing sterile air flow into the fixed box 9, opening a valve 26 on a discharge pipe 22, discharging the purified umbilical cord mesenchymal stem cells in the middle of the purification barrel 3 from the discharge pipe 22, after the discharge of the umbilical cord mesenchymal stem cells is finished, closing a booster pump 18, a motor I2 and a motor II 4, cleaning impurities generated in the purification process of the umbilical cord mesenchymal stem cells, cleaning purification equipment of the umbilical cord mesenchymal stem cells, and cutting off a power supply.
Example two:
as shown in fig. 5, a purification equipment of umbilical cord mesenchymal stem cells, includes box 1, motor 2, purification bucket 3, motor two 4, rotating gear 5 and planetary gear 6, its characterized in that: the purification device is characterized in that the box body 1 is arranged on a support 7, the top of the box body 1 is provided with a feeding pipe 8, the bottom of the box body 1 is provided with a first bearing 10, the top of the inside of the box body 1 is provided with a fixed box 9, the bottom of the fixed box 9 is provided with a fixed ring 12, a second bearing 11 and a blanking pipe 13, the first motor 2 is arranged at the bottom of the box body 1, the first motor 2 is provided with a first transmission shaft 20, the first transmission shaft 20 vertically extends into the box body 1 and is provided with a driving wheel 21, the bottom of the first transmission shaft 20 is provided with a discharge pipe 22, the discharge pipe 22 vertically penetrates out of the first bearing 10 and is provided with a valve 26, the second motor 4 is arranged at the top of the box body 1, the second transmission shaft 27 is arranged on the second motor 4 and vertically penetrates through the second bearing 11, the rotary gear 5 is arranged at the bottom end of the second transmission shaft 27, the device comprises a planetary gear 6, a fixed ring 12, a rotating gear 5, a fixed rod 28, a fixed box 9, a sealing cover 17, a feeding pipe 8, a purification barrel 3, a motor I2, a transmission shaft I20, a driving wheel 21 on the transmission shaft I20, a discharging pipe 22, a driven wheel 25 on the purification barrel 3 and the purification barrel 3, wherein the planetary gear 6 is arranged between the fixed ring 12 and the rotating gear 5, the planetary gear 6 is connected with the fixed ring 12 and the rotating gear 5, the fixed rod 28 is arranged on the planetary gear 6, umbilical cord mesenchymal stem cells to be purified are fed into the fixed box 9 from the feeding pipe 8, the umbilical cord mesenchymal stem cells are fed into the purification barrel 3 between the inner wall of the purification barrel 3 and a chromatographic layer 23, the sealing cover 17 covers the feeding pipe 8, the umbilical cord mesenchymal stem cells in the fixed box 9 flow into the purification barrel 3 from a discharging pipe 13 under the action of gravity, the motor I2 drives the transmission shaft I20 and the driving wheel 21 on the transmission shaft I20 to rotate, the driving wheel 21 drives the driven wheel 25 on the discharging pipe 22 to rotate, thereby driving the umbilical cord mesenchymal stem cells in the purification barrel 3 and the purification barrel 3 to rotate, the umbilical cord mesenchymal stem cells on one side of the purification barrel 3 to flow to the middle of the chromatographic layer 23 and the molecular sieve layer 24 to the purification barrel 3, the umbilical cord mesenchymal stem cells are filtered and purified, the motor II 4 drives the transmission shaft II 27 and the rotating gear 5 on the transmission shaft II 27 to rotate, the rotating gear 5 drives the planetary gear 6 and the fixing rod 28 on the planetary gear 6 to rotate, the fixing rod 28 moves in the purification barrel 3 between the chromatographic layer 23 and the molecular sieve layer 24, the umbilical cord mesenchymal stem cells between the chromatographic layer 23 and the molecular sieve layer 24 are stirred, the umbilical cord mesenchymal stem cells flow into the discharge pipe 22 through the molecular sieve layer 24, the umbilical cord mesenchymal stem cells are screened and purified through the chromatographic layer 23 and the molecular sieve layer 24, the purification quality of the umbilical cord mesenchymal stem cells is improved, in order to facilitate the cleaning of the purification equipment, the box body 1 can be divided into two sections, the top of the box body 1 can drive the fixing rod 28 to ascend and move out of the purification barrel 3 or stretch the fixing rod 28 into the purification barrel 3, the combination or the division of the purification equipment is realized, is convenient for cleaning the purification barrel 3.
Further, a booster pump 18 is arranged at the top of the box body 1, a gas pipe 19 is arranged on the booster pump 18, one end of the gas pipe 19 extends into the fixed box 9, the booster pump 18 blows bacteria-free gas flow into the fixed box 9 through the gas pipe 19, the sterile gas flow enters the purifying barrel 3 between the inner wall of the purifying barrel 3 and the chromatographic layer 23 through the blanking pipe 13, the pressure of the region is increased, umbilical cord mesenchymal stem cells in the region flow to the region between the chromatographic layer 23 and the molecular sieve layer 24 through the chromatographic layer 23 and flow to the region formed by the molecular sieve layer 24 through the molecular sieve layer 24, the purification of the umbilical cord mesenchymal stem cells is accelerated, the gas pressure of each region in the box body 1 tends to be the same along with the increase of input gas flow, the purification rate of the umbilical cord mesenchymal stem cells tends to be flat, after the purification of the umbilical cord mesenchymal stem cells is completed, the gas pressure in the box body 1 is large, the purified umbilical cord mesenchymal stem cells in the area formed by the molecular sieve layer 24 are quickly discharged from the discharge pipe 22, and the purified umbilical cord mesenchymal stem cells in other areas quickly flow into the area formed by the molecular sieve under the action of airflow pressure to form the umbilicus again.
Furthermore, a positioning plate 14 is arranged on the inner wall of the box body 1, a connecting shaft 15 is arranged between the positioning plate 14 and the positioning plate 14, an auxiliary wheel 16 is arranged on a connecting shaft 15 between the positioning plate 14 and the positioning plate 14, the auxiliary wheel 16 is connected with the outer wall of the purification barrel 3, a motor I2 drives a transmission shaft I20 and a driving wheel 21 on the transmission shaft I20 to rotate, the driving wheel 21 drives a driven wheel 25 on a discharge pipe 22 to rotate, thereby driving the purification barrel 3 and the umbilical cord mesenchymal stem cells in the purification barrel 3 to rotate, leading the umbilical cord mesenchymal stem cells at one side of the chromatographic layer 23 to flow to the middle part of the purification barrel 3 through the chromatographic layer 23 and the molecular sieve layer 24, carry out the filtration purification to umbilical cord mesenchymal stem cell, can carry out spacingly through auxiliary wheel 16 to the purification bucket 3 of rotation in-process, improve the stability of purification bucket 3 in rotation process, and then improve the stability of purification bucket 3 in the course of the work.
Further, the row in box 1 arrange and be provided with on the material pipe 22 from the driving wheel 25 to will be connected from driving wheel 25 and the action wheel 21 on the transmission shaft 20, the action wheel 21 that motor 2 drove transmission shaft 20 and transmission shaft 20 is rotatory, action wheel 21 drives row and arranges the rotation of driving wheel 25 on the material pipe 22, thereby it is rotatory to drive umbilical cord mesenchymal stem cell in purification bucket 3 and the purification bucket 3, makes the umbilical cord mesenchymal stem cell of chromatography layer 23 one side flow to purification bucket 3 middle parts through chromatography layer 23, molecular sieve layer 24, filters the purification to umbilical cord mesenchymal stem cell.
Further, a chromatography layer 23 and a molecular sieve layer 24 are arranged in the purification barrel 3, the bottom end of the blanking pipe 13 extends into the purification barrel 3 between the inner wall of the purification barrel 3 and the chromatography layer 23, umbilical cord mesenchymal stem cells to be purified are added into the fixed box 9 from the feeding pipe 8, the sealing cover 17 covers the feeding pipe 8 after the umbilical cord mesenchymal stem cells are added, the umbilical cord mesenchymal stem cells in the fixed box 9 flow into the purification barrel 3 between the inner wall of the purification barrel 3 and the chromatography layer 23 from the blanking pipe 13 under the action of gravity, the motor I2 drives the transmission shaft I20 and the driving wheel 21 on the transmission shaft I20 to rotate, the driving wheel 21 drives the driven wheel 25 on the discharging pipe 22 to rotate, so that the purification barrel 3 and the umbilical cord mesenchymal stem cells in the purification barrel 3 are driven to rotate, the umbilical cord mesenchymal stem cells on one side of the chromatography layer 23 flow to the middle of the purification barrel 3 through the chromatography layer 23 and the molecular sieve layer 24, the umbilical cord mesenchymal stem cells are filtered and purified, the motor II 4 drives the transmission shaft II 27 and the rotating gear 5 on the transmission shaft II 27 to rotate, the rotating gear 5 drives the planetary gear 6 and the fixing rod 28 on the planetary gear 6 to rotate, the fixing rod 28 moves in the purification barrel 3 between the chromatography layer 23 and the molecular sieve layer 24, the umbilical cord mesenchymal stem cells between the chromatography layer 23 and the molecular sieve layer 24 are stirred, the umbilical cord mesenchymal stem cells flow into the discharge pipe 22 through the molecular sieve layer 24, and the umbilical cord mesenchymal stem cells are screened and purified through the chromatography layer 23 and the molecular sieve layer 24, so that the purification quality of the umbilical cord mesenchymal stem cells is improved.
Further, planetary gear 6, its quantity is 3 to stretch into purification bucket 3 between chromatography layer 23 and molecular sieve layer 24 with dead lever 28 bottom on the planetary gear 6, motor two 4 drives the second 27 of transmission shaft and the rotatory gear 5 on the second 27 of transmission shaft is rotatory, rotatory gear 5 drives planetary gear 6 and the dead lever 28 on the planetary gear 6 rotatory, make dead lever 28 move in purification bucket 3 between chromatography layer 23 and molecular sieve layer 24, a plurality of planetary gear 6 drive many dead levers 28 stir umbilical cord mesenchymal stem cell in purification bucket 3, carry out abundant filtration purification to umbilical cord mesenchymal stem cell, improve umbilical cord mesenchymal stem cell's purification quality.
Further, motor 2 and motor two 4 set up to syntropy or reverse rotation's structure, motor 2 provides power for the rotation of purification bucket 3, motor two 4 provides power for planetary gear 6's rotation, with purification bucket 3 and planetary gear 6 syntropy or reverse rotation, can make dead lever 28 on the planetary gear 6 present irregular motion in purification bucket 3, carry out abundant stirring to the umbilical cord mesenchymal stem cell in the purification bucket 3 and handle, carry out abundant purification to umbilical cord mesenchymal stem cell, the purification quality of reinforcing umbilical cord mesenchymal stem cell.
Further, 8 tops of inlet pipe on box 1 be provided with sealed lid 17, through threaded connection between sealed lid 17 and the inlet pipe 8, open sealed lid 17, inject umbilical cord mesenchymal stem cell into through inlet pipe 8 in to fixed case 9, after injecting into umbilical cord mesenchymal stem cell, be connected sealed lid 17 and inlet pipe 8, improve the leakproofness of purification equipment, avoid receiving the pollution to the umbilical cord mesenchymal stem cell among the purification process, improved the purification effect of umbilical cord mesenchymal stem cell.
Further, the rotating gear 5 on be provided with connecting rod 29 to be provided with puddler 30 on connecting rod 29, motor two 4 drives the second 27 of transmission shaft and the rotating gear 5 on the second 27 of transmission shaft rotatory, and rotating gear 5 drives the puddler 30 on connecting rod 29 and the connecting rod 29 rotatory, makes puddler 30 stir the umbilical cord mesenchymal stem cell after the purification in the molecular sieve layer 24 constitution space, makes the umbilical cord mesenchymal stem cell after the purification evenly distributed, improves the purification quality of umbilical cord mesenchymal stem cell.
An operation method of a purification device suitable for umbilical cord mesenchymal stem cells is characterized in that: the operation method comprises the following steps:
firstly, the method comprises the following steps: transferring the purification equipment of the umbilical cord mesenchymal stem cells to a proper position, and checking whether the line of the purification equipment of the umbilical cord mesenchymal stem cells is normal or not, and whether the motor I2, the motor II 4 and the booster pump 18 can work normally or not;
secondly, the method comprises the following steps: connecting a first motor 2, a second motor 4 and a booster pump 18 with an external control system, and connecting the control system with a power supply;
thirdly, the method comprises the following steps: closing a valve 26 on a discharging pipe 22, opening a sealing cover 17 on a feeding pipe 8, adding umbilical cord mesenchymal stem cells to be purified into a fixed box 9 from the feeding pipe 8, covering the feeding pipe 8 with the sealing cover 17 after the umbilical cord mesenchymal stem cells are added, and allowing the umbilical cord mesenchymal stem cells in the fixed box 9 to flow into the purifying barrel 3 between the inner wall of the purifying barrel 3 and the chromatographic layer 23 from a blanking pipe 13 under the action of gravity;
fourthly: starting the first motor 2 and the second motor 4, wherein the first motor 2 drives the first transmission shaft 20 and the driving wheel 21 on the first transmission shaft 20 to rotate, the driving wheel 21 drives the driven wheel 25 on the discharge pipe 22 to rotate, thereby driving the purification barrel 3 and the umbilical cord mesenchymal stem cells in the purification barrel 3 to rotate, leading the umbilical cord mesenchymal stem cells at one side of the chromatographic layer 23 to flow to the middle part of the purification barrel 3 through the chromatographic layer 23 and the molecular sieve layer 24, filtering and purifying the umbilical cord mesenchymal stem cells, driving a second transmission shaft 27 and a rotating gear 5 on the second transmission shaft 27 to rotate by a second motor 4, driving a planetary gear 6 and a fixing rod 28 on the planetary gear 6 to rotate by the rotating gear 5, enabling the fixing rod 28 to move in the purifying barrel 3 between the chromatographic layer 23 and the molecular sieve layer 24, stirring the umbilical cord mesenchymal stem cells between the chromatographic layer 23 and the molecular sieve layer 24, and enabling the umbilical cord mesenchymal stem cells to flow into the discharge pipe 22 through the molecular sieve layer 24;
fifth, the method comprises the following steps: starting the booster pump 18, wherein the booster pump 18 blows sterile air flow into the fixed box 9 through the air conveying pipe 19 to enhance the air pressure in the fixed box 9 and the box body 1, so that the umbilical cord mesenchymal stem cells in the purification barrel 3 can flow from the edge of the inner wall of the purification barrel 3 to the middle part;
sixth: after the concentration of the umbilical cord mesenchymal stem cells is finished, continuously blowing sterile air flow into the fixed box 9, opening a valve 26 on a discharge pipe 22, discharging the purified umbilical cord mesenchymal stem cells in the middle of the purification barrel 3 from the discharge pipe 22, after the discharge of the umbilical cord mesenchymal stem cells is finished, closing a booster pump 18, a motor I2 and a motor II 4, cleaning impurities generated in the purification process of the umbilical cord mesenchymal stem cells, cleaning purification equipment of the umbilical cord mesenchymal stem cells, and cutting off a power supply.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
The parts not involved in the present invention are the same as or can be implemented using the prior art.

Claims (9)

1. The utility model provides a purification equipment of umbilical cord mesenchymal stem cell, includes box, motor one, purification bucket, motor two, rotating gear and planetary gear, its characterized in that: the purifying barrel is characterized in that the box body is arranged on a support, the top of the box body is provided with a feeding pipe, the bottom of the box body is provided with a bearing I, the top of the box body is provided with a fixed box, the bottom of the fixed box is provided with a fixed ring, a bearing II and a blanking pipe, the motor I is arranged at the bottom of the box body, the motor I is provided with a transmission shaft I, the transmission shaft I vertically extends into the box body, the transmission shaft I is provided with a driving wheel, the bottom of the purifying barrel is provided with a discharging pipe, the discharging pipe vertically penetrates out of the bearing I, a valve is arranged on the discharging pipe, the motor II is arranged at the top of the box body, the motor II is provided with a transmission shaft II, the transmission shaft II vertically penetrates through the bearing II, the rotating gear is arranged at the bottom end of the transmission shaft II, the planetary gear is arranged between the fixed ring and the rotating gear, and is connected with the fixed ring and the rotating gear, and a fixed rod is arranged on the planetary gear.
2. The apparatus for purifying umbilical cord mesenchymal stem cells according to claim 1, wherein: the top of the box body is provided with a booster pump, a gas pipe is arranged on the booster pump, and one end of the gas pipe extends into the fixed box.
3. The apparatus for purifying umbilical cord mesenchymal stem cells according to claim 1, wherein: the inner wall of the box body is provided with a positioning plate, a connecting shaft is arranged between the positioning plate and the positioning plate, and an auxiliary wheel is arranged on the connecting shaft between the positioning plate and is connected with the outer wall of the purification barrel.
4. The apparatus for purifying umbilical cord mesenchymal stem cells according to claim 1, wherein: and a driven wheel is arranged on the discharging pipe in the box body and is connected with a driving wheel on the first transmission shaft.
5. The apparatus for purifying umbilical cord mesenchymal stem cells according to claim 1, wherein: the purification bucket in be provided with chromatography layer, molecular sieve layer to stretch into the purification bucket between purification bucket inner wall and the chromatography layer with blanking pipe bottom.
6. The apparatus for purifying umbilical cord mesenchymal stem cells according to claim 1, wherein: the number of the planetary gears is 2-6, and the bottom ends of fixed rods on the planetary gears extend into a purification barrel between the chromatography layer and the molecular sieve layer.
7. The apparatus for purifying umbilical cord mesenchymal stem cells according to claim 1, wherein: the first motor and the second motor are arranged to rotate in the same direction or in opposite directions.
8. The apparatus for purifying umbilical cord mesenchymal stem cells according to claim 1, wherein: the top end of the feeding pipe on the box body is provided with a sealing cover.
9. An operating method of an apparatus for purifying umbilical cord mesenchymal stem cells suitable for use according to any one of claims 1 to 8, wherein: the operation method comprises the following steps:
firstly, the method comprises the following steps: transferring the purification equipment of the umbilical cord mesenchymal stem cells to a proper position, and checking whether the line of the purification equipment of the umbilical cord mesenchymal stem cells is normal or not, and whether the motor I, the motor II and the booster pump can work normally or not;
secondly, the method comprises the following steps: connecting the motor I, the motor II and the booster pump with an external control system, and connecting the control system with a power supply;
thirdly, the method comprises the following steps: closing a valve on the discharging pipe, opening a sealing cover on the feeding pipe, adding the umbilical cord mesenchymal stem cells to be purified into the fixing box from the feeding pipe, covering the feeding pipe with the sealing cover after the umbilical cord mesenchymal stem cells are added, and allowing the umbilical cord mesenchymal stem cells in the fixing box to flow into the purifying barrel between the inner wall of the purifying barrel and the chromatographic layer from the blanking pipe under the action of gravity;
fourthly: starting a motor I and a motor II, wherein the motor I drives a transmission shaft I and a driving wheel on the transmission shaft I to rotate, the driving wheel drives a driven wheel on a discharge pipe to rotate, so that the purification barrel and the umbilical cord mesenchymal stem cells in the purification barrel are driven to rotate, the umbilical cord mesenchymal stem cells on one side of a chromatography layer flow to the middle part of the purification barrel through the chromatography layer and a molecular sieve layer, the umbilical cord mesenchymal stem cells are filtered and purified, the motor II drives a transmission shaft II and a rotating gear on the transmission shaft II to rotate, the rotating gear drives a planetary gear and a fixed rod on the planetary gear to rotate, the fixed rod moves in the purification barrel between the chromatography layer and the molecular sieve layer, the umbilical cord mesenchymal stem cells between the chromatography layer and the molecular sieve layer are stirred, and the umbilical cord mesenchymal stem cells flow to the discharge pipe through the molecular sieve layer;
fifth, the method comprises the following steps: starting a booster pump, wherein the booster pump blows sterile air flow into the fixed box through a gas pipe to enhance the air pressure in the fixed box and the box body, so that the umbilical cord mesenchymal stem cells in the purification barrel can flow from the edge of the inner wall of the purification barrel to the middle part;
sixth: after the concentration of the umbilical cord mesenchymal stem cells is finished, continuously blowing sterile air flow into the fixed box, opening a valve on a discharge pipe, discharging the purified umbilical cord mesenchymal stem cells in the middle of the purification barrel from the discharge pipe, after the discharge of the umbilical cord mesenchymal stem cells is finished, closing a booster pump, a motor I and a motor II, cleaning impurities generated in the purification process of the umbilical cord mesenchymal stem cells, cleaning purification equipment of the umbilical cord mesenchymal stem cells, and cutting off a power supply.
CN202111426837.9A 2021-11-27 2021-11-27 Purification equipment for umbilical cord mesenchymal stem cells and operation method thereof Pending CN114058483A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111426837.9A CN114058483A (en) 2021-11-27 2021-11-27 Purification equipment for umbilical cord mesenchymal stem cells and operation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111426837.9A CN114058483A (en) 2021-11-27 2021-11-27 Purification equipment for umbilical cord mesenchymal stem cells and operation method thereof

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Publication Number Publication Date
CN114058483A true CN114058483A (en) 2022-02-18

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111426837.9A Pending CN114058483A (en) 2021-11-27 2021-11-27 Purification equipment for umbilical cord mesenchymal stem cells and operation method thereof

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Country Link
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